screenseqscommand.cpp

   1 /*
   2  *  screenseqscommand.cpp
   3  *  Mothur
   4  *
   5  *  Created by Pat Schloss on 6/3/09.
   6  *  Copyright 2009 Patrick D. Schloss. All rights reserved.
   7  *
   8  */
   9
  10 #include "screenseqscommand.h"
  11 #include "sequence.hpp"
  12
  13 //***************************************************************************************************************
  14
  15 ScreenSeqsCommand::ScreenSeqsCommand(string option){
  16         try {
  17                 abort = false;
  18
  19                 //allow user to run help
  20                 if(option == "help") { help(); abort = true; }
  21
  22                 else {
  23                         //valid paramters for this command
  24                         string AlignArray[] =  {"fasta", "start", "end", "maxambig", "maxhomop", "minlength", "maxlength",
  25                                                                         "name", "group", "alignreport","outputdir","inputdir"};
  26                         vector<string> myArray (AlignArray, AlignArray+(sizeof(AlignArray)/sizeof(string)));
  27
  28                         OptionParser parser(option);
  29                         map<string,string> parameters = parser.getParameters();
  30
  31                         ValidParameters validParameter;
  32                         map<string,string>::iterator it;
  33
  34                         //check to make sure all parameters are valid for command
  35                         for (it = parameters.begin(); it != parameters.end(); it++) {
  36                                 if (validParameter.isValidParameter(it->first, myArray, it->second) != true) {  abort = true;  }
  37                         }
  38
  39                         //if the user changes the input directory command factory will send this info to us in the output parameter
  40                         string inputDir = validParameter.validFile(parameters, "inputdir", false);
  41                         if (inputDir == "not found"){   inputDir = "";          }
  42                         else {
  43                                 string path;
  44                                 it = parameters.find("fasta");
  45                                 //user has given a template file
  46                                 if(it != parameters.end()){
  47                                         path = hasPath(it->second);
  48                                         //if the user has not given a path then, add inputdir. else leave path alone.
  49                                         if (path == "") {       parameters["fasta"] = inputDir + it->second;            }
  50                                 }
  51
  52                                 it = parameters.find("group");
  53                                 //user has given a template file
  54                                 if(it != parameters.end()){
  55                                         path = hasPath(it->second);
  56                                         //if the user has not given a path then, add inputdir. else leave path alone.
  57                                         if (path == "") {       parameters["group"] = inputDir + it->second;            }
  58                                 }
  59
  60                                 it = parameters.find("name");
  61                                 //user has given a template file
  62                                 if(it != parameters.end()){
  63                                         path = hasPath(it->second);
  64                                         //if the user has not given a path then, add inputdir. else leave path alone.
  65                                         if (path == "") {       parameters["name"] = inputDir + it->second;             }
  66                                 }
  67
  68                                 it = parameters.find("alignreport");
  69                                 //user has given a template file
  70                                 if(it != parameters.end()){
  71                                         path = hasPath(it->second);
  72                                         //if the user has not given a path then, add inputdir. else leave path alone.
  73                                         if (path == "") {       parameters["alignreport"] = inputDir + it->second;              }
  74                                 }
  75                         }
  76
  77                         //check for required parameters
  78                         fastafile = validParameter.validFile(parameters, "fasta", true);
  79                         if (fastafile == "not found") { mothurOut("fasta is a required parameter for the screen.seqs command."); mothurOutEndLine(); abort = true; }
  80                         else if (fastafile == "not open") { abort = true; }
  81
  82                         groupfile = validParameter.validFile(parameters, "group", true);
  83                         if (groupfile == "not open") { abort = true; }
  84                         else if (groupfile == "not found") { groupfile = ""; }
  85
  86                         namefile = validParameter.validFile(parameters, "name", true);
  87                         if (namefile == "not open") { abort = true; }
  88                         else if (namefile == "not found") { namefile = ""; }
  89
  90                         alignreport = validParameter.validFile(parameters, "alignreport", true);
  91                         if (alignreport == "not open") { abort = true; }
  92                         else if (alignreport == "not found") { alignreport = ""; }
  93
  94                         //if the user changes the output directory command factory will send this info to us in the output parameter
  95                         outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){
  96                                 outputDir = "";
  97                                 outputDir += hasPath(fastafile); //if user entered a file with a path then preserve it
  98                         }
  99
 100                         //check for optional parameter and set defaults
 101                         // ...at some point should added some additional type checking...
 102                         string temp;
 103                         temp = validParameter.validFile(parameters, "start", false);            if (temp == "not found") { temp = "-1"; }
 104                         convert(temp, startPos);
 105
 106                         temp = validParameter.validFile(parameters, "end", false);                      if (temp == "not found") { temp = "-1"; }
 107                         convert(temp, endPos);
 108
 109                         temp = validParameter.validFile(parameters, "maxambig", false);         if (temp == "not found") { temp = "-1"; }
 110                         convert(temp, maxAmbig);
 111
 112                         temp = validParameter.validFile(parameters, "maxhomop", false);         if (temp == "not found") { temp = "-1"; }
 113                         convert(temp, maxHomoP);
 114
 115                         temp = validParameter.validFile(parameters, "minlength", false);        if (temp == "not found") { temp = "-1"; }
 116                         convert(temp, minLength);
 117
 118                         temp = validParameter.validFile(parameters, "maxlength", false);        if (temp == "not found") { temp = "-1"; }
 119                         convert(temp, maxLength);
 120                 }
 121
 122         }
 123         catch(exception& e) {
 124                 errorOut(e, "ScreenSeqsCommand", "ScreenSeqsCommand");
 125                 exit(1);
 126         }
 127 }
 128 //**********************************************************************************************************************
 129
 130 void ScreenSeqsCommand::help(){
 131         try {
 132                 mothurOut("The screen.seqs command reads a fastafile and creates .....\n");
 133                 mothurOut("The screen.seqs command parameters are fasta, start, end, maxambig, maxhomop, minlength, maxlength, name, and group.\n");
 134                 mothurOut("The fasta parameter is required.\n");
 135                 mothurOut("The start parameter .... The default is -1.\n");
 136                 mothurOut("The end parameter .... The default is -1.\n");
 137                 mothurOut("The maxambig parameter .... The default is -1.\n");
 138                 mothurOut("The maxhomop parameter .... The default is -1.\n");
 139                 mothurOut("The minlength parameter .... The default is -1.\n");
 140                 mothurOut("The maxlength parameter .... The default is -1.\n");
 141                 mothurOut("The name parameter allows you to provide a namesfile, and the group parameter allows you to provide a groupfile.\n");
 142                 mothurOut("The screen.seqs command should be in the following format: \n");
 143                 mothurOut("screen.seqs(fasta=yourFastaFile, name=youNameFile, group=yourGroupFIle, start=yourStart, end=yourEnd, maxambig=yourMaxambig,  \n");
 144                 mothurOut("maxhomop=yourMaxhomop, minlength=youMinlength, maxlength=yourMaxlength)  \n");
 145                 mothurOut("Example screen.seqs(fasta=abrecovery.fasta, name=abrecovery.names, group=abrecovery.groups, start=..., end=..., maxambig=..., maxhomop=..., minlength=..., maxlength=...).\n");
 146                 mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFasta).\n\n");
 147
 148         }
 149         catch(exception& e) {
 150                 errorOut(e, "ScreenSeqsCommand", "help");
 151                 exit(1);
 152         }
 153 }
 154
 155 //***************************************************************************************************************
 156
 157 ScreenSeqsCommand::~ScreenSeqsCommand(){        /*      do nothing      */      }
 158
 159 //***************************************************************************************************************
 160
 161 int ScreenSeqsCommand::execute(){
 162         try{
 163
 164                 if (abort == true) { return 0; }
 165
 166                 ifstream inFASTA;
 167                 openInputFile(fastafile, inFASTA);
 168
 169                 set<string> badSeqNames;
 170
 171                 string goodSeqFile = outputDir + getRootName(getSimpleName(fastafile)) + "good" + getExtension(fastafile);
 172                 string badSeqFile =  outputDir + getRootName(getSimpleName(fastafile)) + "bad" + getExtension(fastafile);
 173
 174                 ofstream goodSeqOut;    openOutputFile(goodSeqFile, goodSeqOut);
 175                 ofstream badSeqOut;             openOutputFile(badSeqFile, badSeqOut);
 176
 177                 while(!inFASTA.eof()){
 178                         Sequence currSeq(inFASTA);
 179                         if (currSeq.getName() != "") {
 180                                 bool goodSeq = 1;               //      innocent until proven guilty
 181                                 if(goodSeq == 1 && startPos != -1 && startPos < currSeq.getStartPos())                  {       goodSeq = 0;    }
 182                                 if(goodSeq == 1 && endPos != -1 && endPos > currSeq.getEndPos())                                {       goodSeq = 0;    }
 183                                 if(goodSeq == 1 && maxAmbig != -1 && maxAmbig < currSeq.getAmbigBases())                {       goodSeq = 0;    }
 184                                 if(goodSeq == 1 && maxHomoP != -1 && maxHomoP < currSeq.getLongHomoPolymer())   {       goodSeq = 0;    }
 185                                 if(goodSeq == 1 && minLength != -1 && minLength > currSeq.getNumBases())                {       goodSeq = 0;    }
 186                                 if(goodSeq == 1 && maxLength != -1 && maxLength < currSeq.getNumBases())                {       goodSeq = 0;    }
 187
 188                                 if(goodSeq == 1){
 189                                         currSeq.printSequence(goodSeqOut);
 190                                 }
 191                                 else{
 192                                         currSeq.printSequence(badSeqOut);
 193                                         badSeqNames.insert(currSeq.getName());
 194                                 }
 195                         }
 196                         gobble(inFASTA);
 197                 }
 198                 if(namefile != "" && groupfile != "")   {       screenNameGroupFile(badSeqNames);       }       // this screens both names and groups
 199                 else if(namefile != "")                                 {       screenNameGroupFile(badSeqNames);       }
 200                 else if(groupfile != "")                                {       screenGroupFile(badSeqNames);           }       // this screens just the groups
 201                 if(alignreport != "")                                   {       screenAlignReport(badSeqNames);         }
 202
 203                 goodSeqOut.close();
 204                 badSeqOut.close();
 205                 inFASTA.close();
 206                 return 0;
 207         }
 208         catch(exception& e) {
 209                 errorOut(e, "ScreenSeqsCommand", "execute");
 210                 exit(1);
 211         }
 212 }
 213
 214 //***************************************************************************************************************
 215
 216 void ScreenSeqsCommand::screenNameGroupFile(set<string> badSeqNames){
 217
 218         ifstream inputNames;
 219         openInputFile(namefile, inputNames);
 220         set<string> badSeqGroups;
 221         string seqName, seqList, group;
 222         set<string>::iterator it;
 223
 224         string goodNameFile = outputDir + getRootName(getSimpleName(namefile)) + "good" + getExtension(namefile);
 225         string badNameFile = outputDir + getRootName(getSimpleName(namefile)) + "bad" + getExtension(namefile);
 226
 227         ofstream goodNameOut;   openOutputFile(goodNameFile, goodNameOut);
 228         ofstream badNameOut;    openOutputFile(badNameFile, badNameOut);
 229
 230         while(!inputNames.eof()){
 231                 inputNames >> seqName >> seqList;
 232                 it = badSeqNames.find(seqName);
 233
 234                 if(it != badSeqNames.end()){
 235                         badSeqNames.erase(it);
 236                         badNameOut << seqName << '\t' << seqList << endl;
 237                         if(namefile != ""){
 238                                 int start = 0;
 239                                 for(int i=0;i<seqList.length();i++){
 240                                         if(seqList[i] == ','){
 241                                                 badSeqGroups.insert(seqList.substr(start,i-start));
 242                                                 start = i+1;
 243                                         }
 244                                 }
 245                                 badSeqGroups.insert(seqList.substr(start,seqList.length()-start));
 246                         }
 247                 }
 248                 else{
 249                         goodNameOut << seqName << '\t' << seqList << endl;
 250                 }
 251                 gobble(inputNames);
 252         }
 253         inputNames.close();
 254         goodNameOut.close();
 255         badNameOut.close();
 256
 257         //we were unable to remove some of the bad sequences
 258         if (badSeqNames.size() != 0) {
 259                 for (it = badSeqNames.begin(); it != badSeqNames.end(); it++) {
 260                         mothurOut("Your namefile does not include the sequence " + *it + " please correct.");
 261                         mothurOutEndLine();
 262                 }
 263         }
 264
 265         if(groupfile != ""){
 266
 267                 ifstream inputGroups;
 268                 openInputFile(groupfile, inputGroups);
 269
 270                 string goodGroupFile = outputDir + getRootName(getSimpleName(groupfile)) + "good" + getExtension(groupfile);
 271                 string badGroupFile = outputDir + getRootName(getSimpleName(groupfile)) + "bad" + getExtension(groupfile);
 272
 273                 ofstream goodGroupOut;  openOutputFile(goodGroupFile, goodGroupOut);
 274                 ofstream badGroupOut;   openOutputFile(badGroupFile, badGroupOut);
 275
 276                 while(!inputGroups.eof()){
 277                         inputGroups >> seqName >> group;
 278
 279                         it = badSeqGroups.find(seqName);
 280
 281                         if(it != badSeqGroups.end()){
 282                                 badSeqGroups.erase(it);
 283                                 badGroupOut << seqName << '\t' << group << endl;
 284                         }
 285                         else{
 286                                 goodGroupOut << seqName << '\t' << group << endl;
 287                         }
 288                         gobble(inputGroups);
 289                 }
 290                 inputGroups.close();
 291                 goodGroupOut.close();
 292                 badGroupOut.close();
 293
 294                 //we were unable to remove some of the bad sequences
 295                 if (badSeqGroups.size() != 0) {
 296                         for (it = badSeqGroups.begin(); it != badSeqGroups.end(); it++) {
 297                                 mothurOut("Your namefile does not include the sequence " + *it + " please correct.");
 298                                 mothurOutEndLine();
 299                         }
 300                 }
 301         }
 302 }
 303
 304 //***************************************************************************************************************
 305
 306 void ScreenSeqsCommand::screenGroupFile(set<string> badSeqNames){
 307
 308         ifstream inputGroups;
 309         openInputFile(groupfile, inputGroups);
 310         string seqName, group;
 311         set<string>::iterator it;
 312
 313         string goodGroupFile = outputDir + getRootName(getSimpleName(groupfile)) + "good" + getExtension(groupfile);
 314         string badGroupFile = outputDir + getRootName(getSimpleName(groupfile)) + "bad" + getExtension(groupfile);
 315
 316         ofstream goodGroupOut;  openOutputFile(goodGroupFile, goodGroupOut);
 317         ofstream badGroupOut;   openOutputFile(badGroupFile, badGroupOut);
 318
 319         while(!inputGroups.eof()){
 320                 inputGroups >> seqName >> group;
 321                 it = badSeqNames.find(seqName);
 322
 323                 if(it != badSeqNames.end()){
 324                         badSeqNames.erase(it);
 325                         badGroupOut << seqName << '\t' << group << endl;
 326                 }
 327                 else{
 328                         goodGroupOut << seqName << '\t' << group << endl;
 329                 }
 330                 gobble(inputGroups);
 331         }
 332
 333         //we were unable to remove some of the bad sequences
 334         if (badSeqNames.size() != 0) {
 335                 for (it = badSeqNames.begin(); it != badSeqNames.end(); it++) {
 336                         mothurOut("Your groupfile does not include the sequence " + *it + " please correct.");
 337                         mothurOutEndLine();
 338                 }
 339         }
 340
 341         inputGroups.close();
 342         goodGroupOut.close();
 343         badGroupOut.close();
 344
 345 }
 346
 347 //***************************************************************************************************************
 348
 349 void ScreenSeqsCommand::screenAlignReport(set<string> badSeqNames){
 350
 351         ifstream inputAlignReport;
 352         openInputFile(alignreport, inputAlignReport);
 353         string seqName, group;
 354         set<string>::iterator it;
 355
 356         string goodAlignReportFile = outputDir + getRootName(getSimpleName(alignreport)) + "good" + getExtension(alignreport);
 357         string badAlignReportFile = outputDir + getRootName(getSimpleName(alignreport)) + "bad" + getExtension(alignreport);
 358
 359         ofstream goodAlignReportOut;    openOutputFile(goodAlignReportFile, goodAlignReportOut);
 360         ofstream badAlignReportOut;             openOutputFile(badAlignReportFile, badAlignReportOut);
 361
 362         while (!inputAlignReport.eof()) {               //      need to copy header
 363                 char c = inputAlignReport.get();
 364                 goodAlignReportOut << c;
 365                 badAlignReportOut << c;
 366                 if (c == 10 || c == 13){        break;  }
 367         }
 368
 369         while(!inputAlignReport.eof()){
 370                 inputAlignReport >> seqName;
 371                 it = badSeqNames.find(seqName);
 372                 string line;
 373                 while (!inputAlignReport.eof()) {               //      need to copy header
 374                         char c = inputAlignReport.get();
 375                         line += c;
 376                         if (c == 10 || c == 13){        break;  }
 377                 }
 378
 379                 if(it != badSeqNames.end()){
 380                         badSeqNames.erase(it);
 381                         badAlignReportOut << seqName << '\t' << line;;
 382                 }
 383                 else{
 384                         goodAlignReportOut << seqName << '\t' << line;
 385                 }
 386                 gobble(inputAlignReport);
 387         }
 388
 389         //we were unable to remove some of the bad sequences
 390         if (badSeqNames.size() != 0) {
 391                 for (it = badSeqNames.begin(); it != badSeqNames.end(); it++) {
 392                         mothurOut("Your file does not include the sequence " + *it + " please correct.");
 393                         mothurOutEndLine();
 394                 }
 395         }
 396
 397         inputAlignReport.close();
 398         goodAlignReportOut.close();
 399         badAlignReportOut.close();
 400
 401 }
 402
 403 //***************************************************************************************************************
 404
 405