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1 /*
2  *  parsimonycommand.cpp
3  *  Mothur
4  *
5  *  Created by Sarah Westcott on 1/26/09.
6  *  Copyright 2009 Schloss Lab UMASS Amherst. All rights reserved.
7  *
8  */
9
10 #include "parsimonycommand.h"
11
12 //**********************************************************************************************************************
13 vector<string> ParsimonyCommand::setParameters(){       
14         try {
15                 CommandParameter ptree("tree", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(ptree);
16                 CommandParameter pgroup("group", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(pgroup);
17                 CommandParameter pname("name", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(pname);
18                 CommandParameter pgroups("groups", "String", "", "", "", "", "",false,false); parameters.push_back(pgroups);
19                 CommandParameter prandom("random", "String", "", "", "", "", "",false,false); parameters.push_back(prandom);
20                 CommandParameter piters("iters", "Number", "", "1000", "", "", "",false,false); parameters.push_back(piters);
21                 CommandParameter pprocessors("processors", "Number", "", "1", "", "", "",false,false); parameters.push_back(pprocessors);
22                 CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
23                 CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir);
24                 
25                 vector<string> myArray;
26                 for (int i = 0; i < parameters.size(); i++) {   myArray.push_back(parameters[i].name);          }
27                 return myArray;
28         }
29         catch(exception& e) {
30                 m->errorOut(e, "ParsimonyCommand", "setParameters");
31                 exit(1);
32         }
33 }
34 //**********************************************************************************************************************
35 string ParsimonyCommand::getHelpString(){       
36         try {
37                 string helpString = "";
38                 helpString += "The parsimony command parameters are tree, group, name, random, groups, processors and iters.  tree parameter is required unless you have valid current tree file or are using random.\n";
39                 helpString += "The groups parameter allows you to specify which of the groups in your groupfile you would like analyzed.  You must enter at least 1 valid group.\n";
40                 helpString += "The group names are separated by dashes.  The iters parameter allows you to specify how many random trees you would like compared to your tree.\n";
41                 helpString += "The parsimony command should be in the following format: parsimony(random=yourOutputFilename, groups=yourGroups, iters=yourIters).\n";
42                 helpString += "The processors parameter allows you to specify the number of processors to use. The default is 1.\n";
43                 helpString += "Example parsimony(random=out, iters=500).\n";
44                 helpString += "The default value for random is "" (meaning you want to use the trees in your inputfile, randomtree=out means you just want the random distribution of trees outputted to out.rd_parsimony),\n";
45                 helpString += "and iters is 1000.  The parsimony command output two files: .parsimony and .psummary their descriptions are in the manual.\n";
46                 helpString += "Note: No spaces between parameter labels (i.e. random), '=' and parameters (i.e.yourOutputFilename).\n";
47                 return helpString;
48         }
49         catch(exception& e) {
50                 m->errorOut(e, "ParsimonyCommand", "getHelpString");
51                 exit(1);
52         }
53 }
54
55 //**********************************************************************************************************************
56 ParsimonyCommand::ParsimonyCommand(){   
57         try {
58                 abort = true; calledHelp = true; 
59                 setParameters();
60                 vector<string> tempOutNames;
61                 outputTypes["parsimony"] = tempOutNames;
62                 outputTypes["psummary"] = tempOutNames;
63         }
64         catch(exception& e) {
65                 m->errorOut(e, "ParsimonyCommand", "ParsimonyCommand");
66                 exit(1);
67         }
68 }
69 /***********************************************************/
70 ParsimonyCommand::ParsimonyCommand(string option)  {
71         try {
72                 abort = false; calledHelp = false;   
73                 Groups.clear();
74                         
75                 //allow user to run help
76                 if(option == "help") { help(); abort = true; calledHelp = true; }
77                 else if(option == "citation") { citation(); abort = true; calledHelp = true;}
78                 
79                 else {
80                         vector<string> myArray = setParameters();
81                         
82                         OptionParser parser(option);
83                         map<string, string> parameters = parser.getParameters();
84                         map<string,string>::iterator it;
85                         
86                         ValidParameters validParameter;
87                 
88                         //check to make sure all parameters are valid for command
89                         for (it = parameters.begin(); it != parameters.end(); it++) { 
90                                 if (validParameter.isValidParameter(it->first, myArray, it->second) != true) {  abort = true;  }
91                         }
92                         
93                         //initialize outputTypes
94                         vector<string> tempOutNames;
95                         outputTypes["parsimony"] = tempOutNames;
96                         outputTypes["psummary"] = tempOutNames;
97                         
98                         //if the user changes the input directory command factory will send this info to us in the output parameter 
99                         string inputDir = validParameter.validFile(parameters, "inputdir", false);              
100                         if (inputDir == "not found"){   inputDir = "";          }
101                         else {
102                                 string path;
103                                 it = parameters.find("tree");
104                                 //user has given a template file
105                                 if(it != parameters.end()){ 
106                                         path = m->hasPath(it->second);
107                                         //if the user has not given a path then, add inputdir. else leave path alone.
108                                         if (path == "") {       parameters["tree"] = inputDir + it->second;             }
109                                 }
110                                 
111                                 it = parameters.find("group");
112                                 //user has given a template file
113                                 if(it != parameters.end()){ 
114                                         path = m->hasPath(it->second);
115                                         //if the user has not given a path then, add inputdir. else leave path alone.
116                                         if (path == "") {       parameters["group"] = inputDir + it->second;            }
117                                 }
118                                 
119                                 it = parameters.find("name");
120                                 //user has given a template file
121                                 if(it != parameters.end()){ 
122                                         path = m->hasPath(it->second);
123                                         //if the user has not given a path then, add inputdir. else leave path alone.
124                                         if (path == "") {       parameters["name"] = inputDir + it->second;             }
125                                 }
126                         }
127                         
128                         m->runParse = true;
129                         m->Groups.clear();
130                         m->namesOfGroups.clear();
131                         m->Treenames.clear();
132                         m->names.clear();
133                         
134                         outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){  outputDir = ""; }
135                         
136                         randomtree = validParameter.validFile(parameters, "random", false);             if (randomtree == "not found") { randomtree = ""; }
137                         
138                         //are you trying to use parsimony without reading a tree or saying you want random distribution
139                         if (randomtree == "")  {
140                                 //check for required parameters
141                                 treefile = validParameter.validFile(parameters, "tree", true);
142                                 if (treefile == "not open") { abort = true; }
143                                 else if (treefile == "not found") {                             //if there is a current design file, use it
144                                         treefile = m->getTreeFile(); 
145                                         if (treefile != "") { m->mothurOut("Using " + treefile + " as input file for the tree parameter."); m->mothurOutEndLine(); }
146                                         else {  m->mothurOut("You have no current tree file and the tree parameter is required."); m->mothurOutEndLine(); abort = true; }                                                               
147                                 }else { m->setTreeFile(treefile); }     
148                                 
149                                 //check for required parameters
150                                 groupfile = validParameter.validFile(parameters, "group", true);
151                                 if (groupfile == "not open") { abort = true; }
152                                 else if (groupfile == "not found") { groupfile = ""; }
153                                 else { m->setGroupFile(groupfile); }
154                                 
155                                 namefile = validParameter.validFile(parameters, "name", true);
156                                 if (namefile == "not open") { abort = true; }
157                                 else if (namefile == "not found") { namefile = ""; }
158                                 else { m->setNameFile(namefile); }
159                         }
160                         
161                         //if the user changes the output directory command factory will send this info to us in the output parameter 
162                         string outputDir = validParameter.validFile(parameters, "outputdir", false);            if (outputDir == "not found"){  outputDir = ""; if (randomtree == "")  { outputDir += m->hasPath(treefile); } }
163                         
164                         //check for optional parameter and set defaults
165                         // ...at some point should added some additional type checking...
166                         groups = validParameter.validFile(parameters, "groups", false);                 
167                         if (groups == "not found") { groups = ""; m->Groups.clear(); }
168                         else { 
169                                 m->splitAtDash(groups, Groups);
170                                 m->Groups = Groups;
171                         }
172                                 
173                         itersString = validParameter.validFile(parameters, "iters", false);                     if (itersString == "not found") { itersString = "1000"; }
174                         convert(itersString, iters); 
175                         
176                         string temp = validParameter.validFile(parameters, "processors", false);        if (temp == "not found"){       temp = m->getProcessors();      }
177                         m->setProcessors(temp);
178                         convert(temp, processors);
179                         
180                 }
181
182         }
183         catch(exception& e) {
184                 m->errorOut(e, "ParsimonyCommand", "ParsimonyCommand");
185                 exit(1);
186         }
187 }
188 /***********************************************************/
189 int ParsimonyCommand::execute() {
190         try {
191         
192                 if (abort == true) { if (calledHelp) { return 0; }  return 2;   }
193                 
194                 
195                 //randomtree will tell us if user had their own treefile or if they just want the random distribution
196                 //user has entered their own tree
197                 if (randomtree == "") { 
198                         
199                         m->setTreeFile(treefile);
200                         
201                         if (groupfile != "") {
202                                 //read in group map info.
203                                 tmap = new TreeMap(groupfile);
204                                 tmap->readMap();
205                         }else{ //fake out by putting everyone in one group
206                                 Tree* tree = new Tree(treefile); delete tree;  //extracts names from tree to make faked out groupmap
207                                 tmap = new TreeMap();
208                                 
209                                 for (int i = 0; i < m->Treenames.size(); i++) { tmap->addSeq(m->Treenames[i], "Group1"); }
210                         }
211                         
212                         if (namefile != "") { readNamesFile(); }
213                         
214                         read = new ReadNewickTree(treefile);
215                         int readOk = read->read(tmap); 
216                         
217                         if (readOk != 0) { m->mothurOut("Read Terminated."); m->mothurOutEndLine(); delete tmap; delete read; return 0; }
218                         
219                         read->AssembleTrees();
220                         T = read->getTrees();
221                         delete read;
222                         
223                         //make sure all files match
224                         //if you provide a namefile we will use the numNames in the namefile as long as the number of unique match the tree names size.
225                         int numNamesInTree;
226                         if (namefile != "")  {  
227                                 if (numUniquesInName == m->Treenames.size()) {  numNamesInTree = nameMap.size();  }
228                                 else {   numNamesInTree = m->Treenames.size();  }
229                         }else {  numNamesInTree = m->Treenames.size();  }
230                         
231                         
232                         //output any names that are in group file but not in tree
233                         if (numNamesInTree < tmap->getNumSeqs()) {
234                                 for (int i = 0; i < tmap->namesOfSeqs.size(); i++) {
235                                         //is that name in the tree?
236                                         int count = 0;
237                                         for (int j = 0; j < m->Treenames.size(); j++) {
238                                                 if (tmap->namesOfSeqs[i] == m->Treenames[j]) { break; } //found it
239                                                 count++;
240                                         }
241                                         
242                                         if (m->control_pressed) { 
243                                                 delete tmap; for (int i = 0; i < T.size(); i++) { delete T[i]; }
244                                                 for (int i = 0; i < outputNames.size(); i++) {  m->mothurRemove(outputNames[i]); } outputTypes.clear();
245                                                 m->Groups.clear();
246                                                 return 0;
247                                         }
248                                         
249                                         //then you did not find it so report it 
250                                         if (count == m->Treenames.size()) { 
251                                                 //if it is in your namefile then don't remove
252                                                 map<string, string>::iterator it = nameMap.find(tmap->namesOfSeqs[i]);
253                                                 
254                                                 if (it == nameMap.end()) {
255                                                         m->mothurOut(tmap->namesOfSeqs[i] + " is in your groupfile and not in your tree. It will be disregarded."); m->mothurOutEndLine();
256                                                         tmap->removeSeq(tmap->namesOfSeqs[i]);
257                                                         i--; //need this because removeSeq removes name from namesOfSeqs
258                                                 }
259                                         }
260                                 }
261                         }
262                                 
263                         if(outputDir == "") { outputDir += m->hasPath(treefile); }
264                         output = new ColumnFile(outputDir + m->getSimpleName(treefile)  +  ".parsimony", itersString);
265                         outputNames.push_back(outputDir + m->getSimpleName(treefile)  +  ".parsimony");
266                         outputTypes["parsimony"].push_back(outputDir + m->getSimpleName(treefile)  +  ".parsimony");
267                                 
268                         sumFile = outputDir + m->getSimpleName(treefile) + ".psummary";
269                         m->openOutputFile(sumFile, outSum);
270                         outputNames.push_back(sumFile);
271                         outputTypes["psummary"].push_back(sumFile);
272                 }else { //user wants random distribution
273                         getUserInput();
274                                 
275                         if(outputDir == "") { outputDir += m->hasPath(randomtree); }
276                         output = new ColumnFile(outputDir+ m->getSimpleName(randomtree), itersString);
277                         outputNames.push_back(outputDir+ m->getSimpleName(randomtree));
278                         outputTypes["parsimony"].push_back(outputDir+ m->getSimpleName(randomtree));
279                 }
280                         
281                 //set users groups to analyze
282                 util = new SharedUtil();
283                 util->setGroups(m->Groups, tmap->namesOfGroups, allGroups, numGroups, "parsimony");     //sets the groups the user wants to analyze
284                 util->getCombos(groupComb, m->Groups, numComp);
285                 delete util;
286                         
287                 if (numGroups == 1) { numComp++; groupComb.push_back(allGroups); }
288                         
289                 pars = new Parsimony(tmap);
290                 counter = 0;
291         
292                 Progress* reading;
293                 reading = new Progress("Comparing to random:", iters);
294                 
295                 if (m->control_pressed) { 
296                         delete reading; delete pars; delete output;
297                         delete tmap; for (int i = 0; i < T.size(); i++) { delete T[i]; }
298                         if (randomtree == "") {  outSum.close();  }
299                         for (int i = 0; i < outputNames.size(); i++) {  m->mothurRemove(outputNames[i]); } outputTypes.clear();
300                         m->Groups.clear();
301                         return 0;
302                 }
303                         
304                 
305                 //get pscore for users tree
306                 userData.resize(numComp,0);  //data = AB, AC, BC, ABC.
307                 randomData.resize(numComp,0);  //data = AB, AC, BC, ABC.
308                 rscoreFreq.resize(numComp);  
309                 uscoreFreq.resize(numComp);  
310                 rCumul.resize(numComp);  
311                 uCumul.resize(numComp);  
312                 userTreeScores.resize(numComp);  
313                 UScoreSig.resize(numComp); 
314                                 
315                 if (randomtree == "") {
316                         //get pscores for users trees
317                         for (int i = 0; i < T.size(); i++) {
318                                 userData = pars->getValues(T[i], processors, outputDir);  //data = AB, AC, BC, ABC.
319                                 
320                                 if (m->control_pressed) { 
321                                         delete reading; delete pars; delete output;
322                                         delete tmap; for (int i = 0; i < T.size(); i++) { delete T[i]; }
323                                         if (randomtree == "") {  outSum.close();  }
324                                         for (int i = 0; i < outputNames.size(); i++) {  m->mothurRemove(outputNames[i]); } outputTypes.clear();
325                                         m->Groups.clear();
326                                         return 0;
327                                 }
328
329
330                                 //output scores for each combination
331                                 for(int k = 0; k < numComp; k++) {
332
333                                         //update uscoreFreq
334                                         map<int,double>::iterator it = uscoreFreq[k].find(userData[k]);
335                                         if (it == uscoreFreq[k].end()) {//new score
336                                                 uscoreFreq[k][userData[k]] = 1;
337                                         }else{ uscoreFreq[k][userData[k]]++; }
338                                         
339                                         //add users score to valid scores
340                                         validScores[userData[k]] = userData[k];
341                                         
342                                         //save score for summary file
343                                         userTreeScores[k].push_back(userData[k]);
344                                 }
345                         }
346                         
347                         //get pscores for random trees
348                         for (int j = 0; j < iters; j++) {
349                                                                 
350                                 //create new tree with same num nodes and leaves as users
351                                 randT = new Tree(tmap);
352
353                                 //create random relationships between nodes
354                                 randT->assembleRandomTree();
355
356                                 //get pscore of random tree
357                                 randomData = pars->getValues(randT, processors, outputDir);
358                                 
359                                 if (m->control_pressed) { 
360                                         delete reading; delete pars; delete output; delete randT;
361                                         if (randomtree == "") {  outSum.close();  }
362                                         for (int i = 0; i < outputNames.size(); i++) {  m->mothurRemove(outputNames[i]); } outputTypes.clear();
363                                         delete tmap; for (int i = 0; i < T.size(); i++) { delete T[i]; }
364                                         m->Groups.clear();
365                                         return 0;
366                                 }
367                                         
368                                 for(int r = 0; r < numComp; r++) {
369                                         //add trees pscore to map of scores
370                                         map<int,double>::iterator it = rscoreFreq[r].find(randomData[r]);
371                                         if (it != rscoreFreq[r].end()) {//already have that score
372                                                 rscoreFreq[r][randomData[r]]++;
373                                         }else{//first time we have seen this score
374                                                 rscoreFreq[r][randomData[r]] = 1;
375                                         }
376                         
377                                         //add randoms score to validscores
378                                         validScores[randomData[r]] = randomData[r];
379                                 }
380                                 
381                                 //update progress bar
382                                 reading->update(j);
383                                 
384                                 delete randT;
385                         }
386
387                 }else {
388                         //get pscores for random trees
389                         for (int j = 0; j < iters; j++) {
390                                                                 
391                                 //create new tree with same num nodes and leaves as users
392                                 randT = new Tree(tmap);
393                                 //create random relationships between nodes
394
395                                 randT->assembleRandomTree();
396                                 
397                                 if (m->control_pressed) { 
398                                         delete reading; delete pars; delete output; delete randT;
399                                         delete tmap; 
400                                         for (int i = 0; i < outputNames.size(); i++) {  m->mothurRemove(outputNames[i]); } outputTypes.clear();
401                                         m->Groups.clear();
402                                         return 0;
403                                 }
404
405
406                                 //get pscore of random tree
407                                 randomData = pars->getValues(randT, processors, outputDir);
408                                 
409                                 if (m->control_pressed) { 
410                                         delete reading; delete pars;  delete output; delete randT;
411                                         delete tmap; 
412                                         for (int i = 0; i < outputNames.size(); i++) {  m->mothurRemove(outputNames[i]); } outputTypes.clear();
413                                         m->Groups.clear();
414                                         return 0;
415                                 }
416                         
417                                 for(int r = 0; r < numComp; r++) {
418                                         //add trees pscore to map of scores
419                                         map<int,double>::iterator it = rscoreFreq[r].find(randomData[r]);
420                                         if (it != rscoreFreq[r].end()) {//already have that score
421                                                 rscoreFreq[r][randomData[r]]++;
422                                         }else{//first time we have seen this score
423                                                 rscoreFreq[r][randomData[r]] = 1;
424                                         }
425                         
426                                         //add randoms score to validscores
427                                         validScores[randomData[r]] = randomData[r];
428                                 }
429                                 
430                                 //update progress bar
431                                 reading->update(j);
432                                 
433                                 delete randT;
434                         }
435                 }
436
437                 for(int a = 0; a < numComp; a++) {
438                         float rcumul = 0.0000;
439                         float ucumul = 0.0000;
440                         //this loop fills the cumulative maps and put 0.0000 in the score freq map to make it easier to print.
441                         for (map<int,double>::iterator it = validScores.begin(); it != validScores.end(); it++) { 
442                                 if (randomtree == "") {
443                                         map<int,double>::iterator it2 = uscoreFreq[a].find(it->first);
444                                         //user data has that score 
445                                         if (it2 != uscoreFreq[a].end()) { uscoreFreq[a][it->first] /= T.size(); ucumul+= it2->second;  }
446                                         else { uscoreFreq[a][it->first] = 0.0000; } //no user trees with that score
447                                         //make uCumul map
448                                         uCumul[a][it->first] = ucumul;
449                                 }
450                         
451                                 //make rscoreFreq map and rCumul
452                                 map<int,double>::iterator it2 = rscoreFreq[a].find(it->first);
453                                 //get percentage of random trees with that info
454                                 if (it2 != rscoreFreq[a].end()) {  rscoreFreq[a][it->first] /= iters; rcumul+= it2->second;  }
455                                 else { rscoreFreq[a][it->first] = 0.0000; } //no random trees with that score
456                                 rCumul[a][it->first] = rcumul;
457                         }
458                         
459                         //find the signifigance of each user trees score when compared to the random trees and save for printing the summary file
460                         for (int h = 0; h < userTreeScores[a].size(); h++) {
461                                 UScoreSig[a].push_back(rCumul[a][userTreeScores[a][h]]);
462                         }
463                 }
464                 
465                 if (m->control_pressed) { 
466                                 delete reading; delete pars; delete output;
467                                 delete tmap; for (int i = 0; i < T.size(); i++) { delete T[i]; }
468                                 if (randomtree == "") {  outSum.close();  }
469                                 for (int i = 0; i < outputNames.size(); i++) {  m->mothurRemove(outputNames[i]); } outputTypes.clear();
470                                 m->Groups.clear();
471                                 return 0;
472                 }
473                 
474                 //finish progress bar
475                 reading->finish();
476                 delete reading;
477
478                 
479                 printParsimonyFile();
480                 if (randomtree == "") { printUSummaryFile(); }
481                 
482                 //reset groups parameter
483                 m->Groups.clear(); 
484                 
485                 delete pars; delete output; 
486                 delete tmap; for (int i = 0; i < T.size(); i++) { delete T[i]; }
487                 
488                 if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) {        m->mothurRemove(outputNames[i]); } outputTypes.clear(); return 0;}
489                 
490                 m->mothurOutEndLine();
491                 m->mothurOut("Output File Names: "); m->mothurOutEndLine();
492                 for (int i = 0; i < outputNames.size(); i++) {  m->mothurOut(outputNames[i]); m->mothurOutEndLine();    }
493                 m->mothurOutEndLine();
494
495                 
496                 return 0;
497                 
498         }
499         catch(exception& e) {
500                 m->errorOut(e, "ParsimonyCommand", "execute");
501                 exit(1);
502         }
503 }
504
505 /***********************************************************/
506 void ParsimonyCommand::printParsimonyFile() {
507         try {
508                 vector<double> data;
509                 vector<string> tags;
510                 
511                 if (randomtree == "") {
512                         tags.push_back("Score"); tags.push_back("UserFreq"); tags.push_back("UserCumul"); tags.push_back("RandFreq"); tags.push_back("RandCumul");
513                 }else {
514                         tags.push_back("Score"); tags.push_back("RandFreq"); tags.push_back("RandCumul");
515                 }
516
517                 for(int a = 0; a < numComp; a++) {
518                         output->initFile(groupComb[a], tags);
519                         //print each line
520                         for (map<int,double>::iterator it = validScores.begin(); it != validScores.end(); it++) { 
521                                 if (randomtree == "") {
522                                         data.push_back(it->first);  data.push_back(uscoreFreq[a][it->first]); data.push_back(uCumul[a][it->first]); data.push_back(rscoreFreq[a][it->first]); data.push_back(rCumul[a][it->first]); 
523                                 }else{
524                                         data.push_back(it->first);  data.push_back(rscoreFreq[a][it->first]); data.push_back(rCumul[a][it->first]); 
525                                 }
526                                 output->output(data);
527                                 data.clear();
528                         } 
529                         output->resetFile();
530                 }
531         }
532         catch(exception& e) {
533                 m->errorOut(e, "ParsimonyCommand", "printParsimonyFile");
534                 exit(1);
535         }
536 }
537 /***********************************************************/
538 int ParsimonyCommand::printUSummaryFile() {
539         try {
540                 //column headers
541                 outSum << "Tree#" << '\t' << "Groups" << '\t'  <<  "ParsScore" << '\t' << "ParsSig" <<  endl;
542                 m->mothurOut("Tree#\tGroups\tParsScore\tParsSig"); m->mothurOutEndLine();
543                 
544                 //format output
545                 outSum.setf(ios::fixed, ios::floatfield); outSum.setf(ios::showpoint);
546                 
547                 
548                 //print each line
549                 for (int i = 0; i< T.size(); i++) {
550                         for(int a = 0; a < numComp; a++) {
551                                 if (m->control_pressed) {  outSum.close(); return 0; }
552                                 if (UScoreSig[a][i] > (1/(float)iters)) {
553                                         outSum << setprecision(6) << i+1 << '\t' << groupComb[a]  << '\t' << userTreeScores[a][i] << setprecision(itersString.length()) << '\t' << UScoreSig[a][i] << endl;
554                                         cout << setprecision(6) << i+1 << '\t' << groupComb[a]  << '\t' << userTreeScores[a][i] << setprecision(itersString.length()) << '\t' << UScoreSig[a][i] << endl;
555                                         m->mothurOutJustToLog(toString(i+1) + "\t" + groupComb[a] + "\t" + toString(userTreeScores[a][i]) + "\t" + toString(UScoreSig[a][i])); m->mothurOutEndLine();
556                                 }else {
557                                         outSum << setprecision(6) << i+1 << '\t' << groupComb[a] << '\t' << userTreeScores[a][i] << setprecision(itersString.length())  << '\t' << "<" << (1/float(iters)) << endl;
558                                         cout << setprecision(6) << i+1 << '\t' << groupComb[a] << '\t' << userTreeScores[a][i] << setprecision(itersString.length()) << '\t' << "<" << (1/float(iters)) << endl;
559                                         m->mothurOutJustToLog(toString(i+1) + "\t" + groupComb[a] + "\t" + toString(userTreeScores[a][i]) + "\t" + toString((1/float(iters)))); m->mothurOutEndLine();
560                                 }
561                         }
562                 }
563                 
564                 outSum.close();
565                 return 0;
566         }
567         catch(exception& e) {
568                 m->errorOut(e, "ParsimonyCommand", "printUSummaryFile");
569                 exit(1);
570         }
571 }
572
573 /***********************************************************/
574 void ParsimonyCommand::getUserInput() {
575         try {
576         
577                 //create treemap
578                 tmap = new TreeMap();
579
580                 m->mothurOut("Please enter the number of groups you would like to analyze: ");
581                 cin >> numGroups;
582                 m->mothurOutJustToLog(toString(numGroups)); m->mothurOutEndLine();
583                                 
584                 int num, count;
585                 count = 1;
586                 numEachGroup.resize(numGroups, 0);  
587                 
588                 for (int i = 1; i <= numGroups; i++) {
589                         m->mothurOut("Please enter the number of sequences in group " + toString(i) +  ": ");
590                         cin >> num;
591                         m->mothurOutJustToLog(toString(num)); m->mothurOutEndLine();
592                                 
593                         //set tmaps seqsPerGroup
594                         tmap->seqsPerGroup[toString(i)] = num;
595                         tmap->namesOfGroups.push_back(toString(i));
596                         
597                         //set tmaps namesOfSeqs
598                         for (int j = 0; j < num; j++) {
599                                 tmap->namesOfSeqs.push_back(toString(count));
600                                 tmap->treemap[toString(count)].groupname = toString(i);
601                                 count++;
602                         }
603                 }
604                 
605                 //clears buffer so next command doesn't have error
606                 string s;       
607                 getline(cin, s);
608                 
609                 m->Treenames = tmap->namesOfSeqs; 
610                 
611         }
612         catch(exception& e) {
613                 m->errorOut(e, "ParsimonyCommand", "getUserInput");
614                 exit(1);
615         }
616 }
617 /*****************************************************************/
618 int ParsimonyCommand::readNamesFile() {
619         try {
620                 m->names.clear();
621                 numUniquesInName = 0;
622                 
623                 ifstream in;
624                 m->openInputFile(namefile, in);
625                 
626                 string first, second;
627                 map<string, string>::iterator itNames;
628                 
629                 while(!in.eof()) {
630                         in >> first >> second; m->gobble(in);
631                         
632                         numUniquesInName++;
633                         
634                         itNames = m->names.find(first);
635                         if (itNames == m->names.end()) {  
636                                 m->names[first] = second; 
637                                 
638                                 //we need a list of names in your namefile to use above when removing extra seqs above so we don't remove them
639                                 vector<string> dupNames;
640                                 m->splitAtComma(second, dupNames);
641                                 
642                                 for (int i = 0; i < dupNames.size(); i++) {     
643                                         nameMap[dupNames[i]] = dupNames[i]; 
644                                         if ((groupfile == "") && (i != 0)) { tmap->addSeq(dupNames[i], "Group1"); } 
645                                 }
646                         }else {  m->mothurOut(first + " has already been seen in namefile, disregarding names file."); m->mothurOutEndLine(); in.close(); m->names.clear(); namefile = ""; return 1; }                  
647                 }
648                 in.close();
649                 
650                 return 0;
651         }
652         catch(exception& e) {
653                 m->errorOut(e, "ParsimonyCommand", "readNamesFile");
654                 exit(1);
655         }
656 }
657 /***********************************************************/
658
659