heatmapsimcommand.cpp

   1 /*
   2  *  heatmapsimcommand.cpp
   3  *  Mothur
   4  *
   5  *  Created by Sarah Westcott on 6/8/09.
   6  *  Copyright 2009 Schloss Lab UMASS Amherst. All rights reserved.
   7  *
   8  */
   9
  10 #include "heatmapsimcommand.h"
  11 #include "sharedjabund.h"
  12 #include "sharedsorabund.h"
  13 #include "sharedjclass.h"
  14 #include "sharedsorclass.h"
  15 #include "sharedjest.h"
  16 #include "sharedsorest.h"
  17 #include "sharedthetayc.h"
  18 #include "sharedthetan.h"
  19 #include "sharedmorisitahorn.h"
  20 #include "sharedbraycurtis.h"
  21
  22
  23 //**********************************************************************************************************************
  24
  25 HeatMapSimCommand::HeatMapSimCommand(string option){
  26         try {
  27                 globaldata = GlobalData::getInstance();
  28                 abort = false;
  29                 allLines = 1;
  30                 labels.clear();
  31                 Groups.clear();
  32                 Estimators.clear();
  33
  34                 //allow user to run help
  35                 if(option == "help") { validCalculator = new ValidCalculators(); help(); abort = true; }
  36
  37                 else {
  38                         //valid paramters for this command
  39                         string AlignArray[] =  {"groups","label", "calc","phylip","column","name","outputdir","inputdir"};
  40                         vector<string> myArray (AlignArray, AlignArray+(sizeof(AlignArray)/sizeof(string)));
  41
  42                         OptionParser parser(option);
  43                         map<string,string> parameters = parser.getParameters();
  44
  45                         ValidParameters validParameter;
  46                         map<string,string>::iterator it;
  47
  48                         //check to make sure all parameters are valid for command
  49                         for (it = parameters.begin(); it != parameters.end(); it++) {
  50                                 if (validParameter.isValidParameter(it->first, myArray, it->second) != true) {  abort = true;  }
  51                         }
  52
  53                         format = "";
  54                         //if the user changes the output directory command factory will send this info to us in the output parameter
  55                         outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){  outputDir = "";         }
  56
  57                         //if the user changes the input directory command factory will send this info to us in the output parameter
  58                         string inputDir = validParameter.validFile(parameters, "inputdir", false);
  59                         if (inputDir == "not found"){   inputDir = "";          }
  60                         else {
  61                                 string path;
  62                                 it = parameters.find("phylip");
  63                                 //user has given a template file
  64                                 if(it != parameters.end()){
  65                                         path = hasPath(it->second);
  66                                         //if the user has not given a path then, add inputdir. else leave path alone.
  67                                         if (path == "") {       parameters["phylip"] = inputDir + it->second;           }
  68                                 }
  69
  70                                 it = parameters.find("column");
  71                                 //user has given a template file
  72                                 if(it != parameters.end()){
  73                                         path = hasPath(it->second);
  74                                         //if the user has not given a path then, add inputdir. else leave path alone.
  75                                         if (path == "") {       parameters["column"] = inputDir + it->second;           }
  76                                 }
  77
  78                                 it = parameters.find("name");
  79                                 //user has given a template file
  80                                 if(it != parameters.end()){
  81                                         path = hasPath(it->second);
  82                                         //if the user has not given a path then, add inputdir. else leave path alone.
  83                                         if (path == "") {       parameters["name"] = inputDir + it->second;             }
  84                                 }
  85                         }
  86
  87                         //required parameters
  88                         phylipfile = validParameter.validFile(parameters, "phylip", true);
  89                         if (phylipfile == "not open") { abort = true; }
  90                         else if (phylipfile == "not found") { phylipfile = ""; }
  91                         else {  format = "phylip";      if (outputDir == "") { outputDir += hasPath(phylipfile); }  }
  92
  93                         columnfile = validParameter.validFile(parameters, "column", true);
  94                         if (columnfile == "not open") { abort = true; }
  95                         else if (columnfile == "not found") { columnfile = ""; }
  96                         else {  format = "column";      if (outputDir == "") { outputDir += hasPath(columnfile); } }
  97
  98                         namefile = validParameter.validFile(parameters, "name", true);
  99                         if (namefile == "not open") { abort = true; }
 100                         else if (namefile == "not found") { namefile = ""; }
 101
 102
 103                         //error checking on files
 104                         if ((globaldata->getSharedFile() == "") && ((phylipfile == "") && (columnfile == "")))  { mothurOut("You must run the read.otu command or provide a distance file before running the heatmap.sim command."); mothurOutEndLine(); abort = true; }
 105                         else if ((phylipfile != "") && (columnfile != "")) { mothurOut("When running the heatmap.sim command with a distance file you may not use both the column and the phylip parameters."); mothurOutEndLine(); abort = true; }
 106
 107                         if (columnfile != "") {
 108                                 if (namefile == "") {  mothurOut("You need to provide a namefile if you are going to use the column format."); mothurOutEndLine(); abort = true; }
 109                         }
 110
 111                         if (format == "") { format = "shared"; }
 112
 113                         //check for optional parameter and set defaults
 114                         // ...at some point should added some additional type checking...
 115                         if (format == "shared") {
 116                                 if (outputDir == "") { outputDir += hasPath(globaldata->getSharedFile()); }
 117
 118                                 label = validParameter.validFile(parameters, "label", false);
 119                                 if (label == "not found") { label = ""; }
 120                                 else {
 121                                         if(label != "all") {  splitAtDash(label, labels);  allLines = 0;  }
 122                                         else { allLines = 1;  }
 123                                 }
 124
 125                                 //if the user has not specified any labels use the ones from read.otu
 126                                 if (label == "") {
 127                                         allLines = globaldata->allLines;
 128                                         labels = globaldata->labels;
 129                                 }
 130
 131                                 calc = validParameter.validFile(parameters, "calc", false);
 132                                 if (calc == "not found") { calc = "jest-thetayc";  }
 133                                 else {
 134                                         if (calc == "default")  {  calc = "jest-thetayc";  }
 135                                 }
 136                                 splitAtDash(calc, Estimators);
 137
 138                                 groups = validParameter.validFile(parameters, "groups", false);
 139                                 if (groups == "not found") { groups = ""; }
 140                                 else {
 141                                         splitAtDash(groups, Groups);
 142                                         globaldata->Groups = Groups;
 143                                 }
 144                         }
 145
 146                         if (abort == false) {
 147                                 validCalculator = new ValidCalculators();
 148
 149                                 int i;
 150                                 for (i=0; i<Estimators.size(); i++) {
 151                                         if (validCalculator->isValidCalculator("heat", Estimators[i]) == true) {
 152                                                 if (Estimators[i] == "jabund") {
 153                                                         heatCalculators.push_back(new JAbund());
 154                                                 }else if (Estimators[i] == "sorabund") {
 155                                                         heatCalculators.push_back(new SorAbund());
 156                                                 }else if (Estimators[i] == "jclass") {
 157                                                         heatCalculators.push_back(new Jclass());
 158                                                 }else if (Estimators[i] == "sorclass") {
 159                                                         heatCalculators.push_back(new SorClass());
 160                                                 }else if (Estimators[i] == "jest") {
 161                                                         heatCalculators.push_back(new Jest());
 162                                                 }else if (Estimators[i] == "sorest") {
 163                                                         heatCalculators.push_back(new SorEst());
 164                                                 }else if (Estimators[i] == "thetayc") {
 165                                                         heatCalculators.push_back(new ThetaYC());
 166                                                 }else if (Estimators[i] == "thetan") {
 167                                                         heatCalculators.push_back(new ThetaN());
 168                                                 }else if (Estimators[i] == "morisitahorn") {
 169                                                         heatCalculators.push_back(new MorHorn());
 170                                                 }else if (Estimators[i] == "braycurtis") {
 171                                                         heatCalculators.push_back(new BrayCurtis());
 172                                                 }
 173                                         }
 174                                 }
 175
 176                         }
 177                 }
 178
 179
 180
 181         }
 182         catch(exception& e) {
 183                 errorOut(e, "HeatMapSimCommand", "HeatMapSimCommand");
 184                 exit(1);
 185         }
 186 }
 187
 188 //**********************************************************************************************************************
 189
 190 void HeatMapSimCommand::help(){
 191         try {
 192                 mothurOut("The heatmap.sim command can only be executed after a successful read.otu command, or by providing a distance file.\n");
 193                 mothurOut("The heatmap.sim command parameters are phylip, column, name, groups, calc and label.  No parameters are required.\n");
 194                 mothurOut("There are two ways to use the heatmap.sim command. The first is with the read.otu command. \n");
 195                 mothurOut("With the read.otu command you may use the groups, label and calc parameters. \n");
 196                 mothurOut("The groups parameter allows you to specify which of the groups in your groupfile you would like included in your heatmap.\n");
 197                 mothurOut("The group names are separated by dashes. The label parameter allows you to select what distance levels you would like a heatmap created for, and is also separated by dashes.\n");
 198                 mothurOut("The heatmap.sim command should be in the following format: heatmap.sim(groups=yourGroups, calc=yourCalc, label=yourLabels).\n");
 199                 mothurOut("Example heatmap.sim(groups=A-B-C, calc=jabund).\n");
 200                 mothurOut("The default value for groups is all the groups in your groupfile, and all labels in your inputfile will be used.\n");
 201                 validCalculator->printCalc("heat", cout);
 202                 mothurOut("The default value for calc is jclass-thetayc.\n");
 203                 mothurOut("The heatmap.sim command outputs a .svg file for each calculator you choose at each label you specify.\n");
 204                 mothurOut("The second way to use the heatmap.sim command is with a distance file representing the distance bewteen your groups. \n");
 205                 mothurOut("Using the command this way, the phylip or column parameter are required, and only one may be used.  If you use a column file the name filename is required. \n");
 206                 mothurOut("The heatmap.sim command should be in the following format: heatmap.sim(phylip=yourDistanceFile).\n");
 207                 mothurOut("Example heatmap.sim(phylip=amazonGroups.dist).\n");
 208                 mothurOut("Note: No spaces between parameter labels (i.e. groups), '=' and parameters (i.e.yourGroups).\n\n");
 209
 210         }
 211         catch(exception& e) {
 212                 errorOut(e, "HeatMapSimCommand", "help");
 213                 exit(1);
 214         }
 215 }
 216
 217 //**********************************************************************************************************************
 218
 219 HeatMapSimCommand::~HeatMapSimCommand(){}
 220
 221 //**********************************************************************************************************************
 222
 223 int HeatMapSimCommand::execute(){
 224         try {
 225
 226                 if (abort == true)  { return 0; }
 227
 228                 heatmap = new HeatMapSim(outputDir);
 229
 230                 if (format == "shared") {
 231                         runCommandShared();
 232                 }else if (format == "phylip") {
 233                         globaldata->inputFileName = phylipfile;
 234                         runCommandDist();
 235                 }else if (format == "column") {
 236                         globaldata->inputFileName = columnfile;
 237                         runCommandDist();
 238                 }
 239
 240                 delete heatmap;
 241                 delete validCalculator;
 242
 243                 return 0;
 244         }
 245         catch(exception& e) {
 246                 errorOut(e, "HeatMapSimCommand", "execute");
 247                 exit(1);
 248         }
 249 }
 250
 251 //**********************************************************************************************************************
 252 int HeatMapSimCommand::runCommandShared() {
 253         try {
 254                 //if the users entered no valid calculators don't execute command
 255                 if (heatCalculators.size() == 0) { mothurOut("No valid calculators."); mothurOutEndLine(); return 0; }
 256
 257                 //you have groups
 258                 read = new ReadOTUFile(globaldata->inputFileName);
 259                 read->read(&*globaldata);
 260
 261                 input = globaldata->ginput;
 262                 lookup = input->getSharedRAbundVectors();
 263                 string lastLabel = lookup[0]->getLabel();
 264
 265                 if (lookup.size() < 2) { mothurOut("You have not provided enough valid groups.  I cannot run the command."); mothurOutEndLine(); return 0;}
 266
 267                 //if the users enters label "0.06" and there is no "0.06" in their file use the next lowest label.
 268                 set<string> processedLabels;
 269                 set<string> userLabels = labels;
 270
 271                 //as long as you are not at the end of the file or done wih the lines you want
 272                 while((lookup[0] != NULL) && ((allLines == 1) || (userLabels.size() != 0))) {
 273
 274                         if(allLines == 1 || labels.count(lookup[0]->getLabel()) == 1){
 275
 276                                 mothurOut(lookup[0]->getLabel()); mothurOutEndLine();
 277                                 heatmap->getPic(lookup, heatCalculators);
 278
 279                                 processedLabels.insert(lookup[0]->getLabel());
 280                                 userLabels.erase(lookup[0]->getLabel());
 281                         }
 282
 283                         if ((anyLabelsToProcess(lookup[0]->getLabel(), userLabels, "") == true) && (processedLabels.count(lastLabel) != 1)) {
 284                                 string saveLabel = lookup[0]->getLabel();
 285
 286                                 for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  }
 287                                 lookup = input->getSharedRAbundVectors(lastLabel);
 288
 289                                 mothurOut(lookup[0]->getLabel()); mothurOutEndLine();
 290                                 heatmap->getPic(lookup, heatCalculators);
 291
 292                                 processedLabels.insert(lookup[0]->getLabel());
 293                                 userLabels.erase(lookup[0]->getLabel());
 294
 295                                 //restore real lastlabel to save below
 296                                 lookup[0]->setLabel(saveLabel);
 297                         }
 298
 299                         //prevent memory leak
 300
 301                         lastLabel = lookup[0]->getLabel();
 302
 303                         //get next line to process
 304                         for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  }
 305                         lookup = input->getSharedRAbundVectors();
 306                 }
 307
 308                 //output error messages about any remaining user labels
 309                 set<string>::iterator it;
 310                 bool needToRun = false;
 311                 for (it = userLabels.begin(); it != userLabels.end(); it++) {
 312                         mothurOut("Your file does not include the label " + *it);
 313                         if (processedLabels.count(lastLabel) != 1) {
 314                                 mothurOut(". I will use " + lastLabel + "."); mothurOutEndLine();
 315                                 needToRun = true;
 316                         }else {
 317                                 mothurOut(". Please refer to " + lastLabel + "."); mothurOutEndLine();
 318                         }
 319                 }
 320
 321                 //run last label if you need to
 322                 if (needToRun == true)  {
 323                         for (int i = 0; i < lookup.size(); i++) {  if (lookup[i] != NULL) { delete lookup[i]; } }
 324                         lookup = input->getSharedRAbundVectors(lastLabel);
 325
 326                         mothurOut(lookup[0]->getLabel()); mothurOutEndLine();
 327                         heatmap->getPic(lookup, heatCalculators);
 328                         for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  }
 329                 }
 330
 331
 332                 //reset groups parameter
 333                 globaldata->Groups.clear();
 334
 335                 delete input;  globaldata->ginput = NULL;
 336                 delete read;
 337
 338                 return 0;
 339         }
 340         catch(exception& e) {
 341                 errorOut(e, "HeatMapSimCommand", "runCommandShared");
 342                 exit(1);
 343         }
 344 }
 345 //**********************************************************************************************************************
 346 int HeatMapSimCommand::runCommandDist() {
 347         try {
 348
 349                 vector< vector<double> > matrix;
 350                 vector<string> names;
 351                 ifstream in;
 352
 353                 //read distance file and create distance vector and names vector
 354                 if (format == "phylip") {
 355                         //read phylip file
 356                         openInputFile(phylipfile, in);
 357
 358                         string name;
 359                         int numSeqs;
 360                         in >> numSeqs >> name;
 361
 362                         //save name
 363                         names.push_back(name);
 364
 365                         //resize the matrix and fill with zeros
 366                         matrix.resize(numSeqs);
 367                         for(int i = 0; i < numSeqs; i++) {
 368                                 matrix[i].resize(numSeqs, 0.0);
 369                         }
 370
 371                         //determine if matrix is square or lower triangle
 372                         //if it is square read the distances for the first sequence
 373                         char d;
 374                         bool square;
 375                         while((d=in.get()) != EOF){
 376
 377                                 //is d a number meaning its square
 378                                 if(isalnum(d)){
 379                                         square = true;
 380                                         in.putback(d);
 381
 382                                         for(int i=0;i<numSeqs;i++){
 383                                                 in >> matrix[0][i];
 384                                         }
 385                                         break;
 386                                 }
 387
 388                                 //is d a line return meaning its lower triangle
 389                                 if(d == '\n'){
 390                                         square = false;
 391                                         break;
 392                                 }
 393                         }
 394
 395                         //read rest of matrix
 396                         if (square == true) {
 397                                 for(int i=1;i<numSeqs;i++){
 398                                         in >> name;
 399                                         names.push_back(name);
 400
 401                                         for(int j=0;j<numSeqs;j++) {  in >> matrix[i][j];  }
 402                                         gobble(in);
 403                                 }
 404                         }else {
 405                                 double dist;
 406                                 for(int i=1;i<numSeqs;i++){
 407                                         in >> name;
 408                                         names.push_back(name);
 409
 410                                         for(int j=0;j<i;j++){
 411                                                 in >> dist;
 412                                                 matrix[i][j] = dist;  matrix[j][i] = dist;
 413                                         }
 414                                         gobble(in);
 415                                 }
 416                         }
 417                         in.close();
 418                 }else {
 419                         //read names file
 420                         NameAssignment* nameMap = new NameAssignment(namefile);
 421                         nameMap->readMap();
 422
 423                         //put names in order in vector
 424                         for (int i = 0; i < nameMap->size(); i++) {
 425                                 names.push_back(nameMap->get(i));
 426                         }
 427
 428                         //resize matrix
 429                         matrix.resize(nameMap->size());
 430                         for (int i = 0; i < nameMap->size(); i++) {
 431                                 matrix[i].resize(nameMap->size(), 0.0);
 432                         }
 433
 434                         //read column file
 435                         string first, second;
 436                         double dist;
 437                         openInputFile(columnfile, in);
 438
 439                         while (!in.eof()) {
 440                                 in >> first >> second >> dist; gobble(in);
 441
 442                                 map<string, int>::iterator itA = nameMap->find(first);
 443                                 map<string, int>::iterator itB = nameMap->find(second);
 444
 445                                 if(itA == nameMap->end()){  cerr << "AAError: Sequence '" << first << "' was not found in the names file, please correct\n"; exit(1);  }
 446                                 if(itB == nameMap->end()){  cerr << "ABError: Sequence '" << second << "' was not found in the names file, please correct\n"; exit(1);  }
 447
 448                                 //save distance
 449                                 matrix[itA->second][itB->second] = dist;
 450                                 matrix[itB->second][itA->second] = dist;
 451                         }
 452                         in.close();
 453
 454                         delete nameMap;
 455                 }
 456
 457
 458                 heatmap->getPic(matrix, names); //vector<vector<double>>, vector<string>
 459
 460                 return 0;
 461         }
 462         catch(exception& e) {
 463                 errorOut(e, "HeatMapSimCommand", "runCommandDist");
 464                 exit(1);
 465         }
 466 }
 467 //**********************************************************************************************************************
 468
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 471
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 473