2 * binsequencecommand.cpp
5 * Created by Sarah Westcott on 4/3/09.
6 * Copyright 2009 Schloss Lab UMASS Amhers. All rights reserved.
10 #include "binsequencecommand.h"
12 //**********************************************************************************************************************
13 BinSeqCommand::BinSeqCommand(string option){
15 globaldata = GlobalData::getInstance();
20 //allow user to run help
21 if(option == "help") { help(); abort = true; }
24 //valid paramters for this command
25 string AlignArray[] = {"fasta","label","name", "group","outputdir","inputdir"};
26 vector<string> myArray (AlignArray, AlignArray+(sizeof(AlignArray)/sizeof(string)));
28 OptionParser parser(option);
29 map<string, string> parameters = parser.getParameters();
31 ValidParameters validParameter;
32 map<string, string>::iterator it;
34 //check to make sure all parameters are valid for command
35 for (it = parameters.begin(); it != parameters.end(); it++) {
36 if (validParameter.isValidParameter(it->first, myArray, it->second) != true) { abort = true; }
39 //if the user changes the output directory command factory will send this info to us in the output parameter
40 outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){
42 outputDir += hasPath(globaldata->getListFile()); //if user entered a file with a path then preserve it
46 //make sure the user has already run the read.otu command
47 if (globaldata->getListFile() == "") {
48 mothurOut("You must read a listfile before running the bin.seqs command.");
53 //if the user changes the input directory command factory will send this info to us in the output parameter
54 string inputDir = validParameter.validFile(parameters, "inputdir", false);
55 if (inputDir == "not found"){ inputDir = ""; }
58 it = parameters.find("fasta");
59 //user has given a template file
60 if(it != parameters.end()){
61 path = hasPath(it->second);
62 //if the user has not given a path then, add inputdir. else leave path alone.
63 if (path == "") { parameters["fasta"] = inputDir + it->second; }
66 it = parameters.find("name");
67 //user has given a template file
68 if(it != parameters.end()){
69 path = hasPath(it->second);
70 //if the user has not given a path then, add inputdir. else leave path alone.
71 if (path == "") { parameters["name"] = inputDir + it->second; }
74 it = parameters.find("group");
75 //user has given a template file
76 if(it != parameters.end()){
77 path = hasPath(it->second);
78 //if the user has not given a path then, add inputdir. else leave path alone.
79 if (path == "") { parameters["group"] = inputDir + it->second; }
84 //check for required parameters
85 fastafile = validParameter.validFile(parameters, "fasta", true);
86 if (fastafile == "not found") { mothurOut("fasta is a required parameter for the bin.seqs command."); mothurOutEndLine(); abort = true; }
87 else if (fastafile == "not open") { abort = true; }
89 //check for optional parameter and set defaults
90 // ...at some point should added some additional type checking...
92 label = validParameter.validFile(parameters, "label", false);
93 if (label == "not found") { label = ""; }
95 if(label != "all") { splitAtDash(label, labels); allLines = 0; }
96 else { allLines = 1; }
99 //if the user has not specified any labels use the ones from read.otu
101 allLines = globaldata->allLines;
102 labels = globaldata->labels;
105 namesfile = validParameter.validFile(parameters, "name", true);
106 if (namesfile == "not open") { abort = true; }
107 else if (namesfile == "not found") { namesfile = ""; }
109 groupfile = validParameter.validFile(parameters, "group", true);
110 if (groupfile == "not open") { abort = true; }
111 else if (groupfile == "not found") { groupfile = ""; }
113 if (abort == false) {
114 // openInputFile(fastafile, in);
115 fasta = new FastaMap();
116 if (groupfile != "") {
117 groupMap = new GroupMap(groupfile);
119 int error = groupMap->readMap();
120 if (error == 1) { delete groupMap; abort = true; }
126 catch(exception& e) {
127 errorOut(e, "BinSeqCommand", "BinSeqCommand");
131 //**********************************************************************************************************************
133 void BinSeqCommand::help(){
135 mothurOut("The bin.seqs command can only be executed after a successful read.otu command of a listfile.\n");
136 mothurOut("The bin.seqs command parameters are fasta, name, label and group. The fasta parameter is required.\n");
137 mothurOut("The label parameter allows you to select what distance levels you would like a output files created for, and are separated by dashes.\n");
138 mothurOut("The bin.seqs command should be in the following format: bin.seqs(fasta=yourFastaFile, name=yourNamesFile, group=yourGroupFile, label=yourLabels).\n");
139 mothurOut("Example bin.seqs(fasta=amazon.fasta, group=amazon.groups, name=amazon.names).\n");
140 mothurOut("The default value for label is all lines in your inputfile.\n");
141 mothurOut("The bin.seqs command outputs a .fasta file for each distance you specify appending the OTU number to each name.\n");
142 mothurOut("If you provide a groupfile, then it also appends the sequences group to the name.\n");
143 mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFastaFile).\n\n");
145 catch(exception& e) {
146 errorOut(e, "BinSeqCommand", "help");
151 //**********************************************************************************************************************
153 BinSeqCommand::~BinSeqCommand(){
154 //made new in execute
155 if (abort == false) {
156 delete input; globaldata->ginput = NULL;
158 globaldata->gListVector = NULL;
160 if (groupfile != "") { delete groupMap; globaldata->gGroupmap = NULL; }
164 //**********************************************************************************************************************
166 int BinSeqCommand::execute(){
168 if (abort == true) { return 0; }
173 fasta->readFastaFile(fastafile);
176 //set format to list so input can get listvector
177 // globaldata->setFormat("list");
179 //if user gave a namesfile then use it
180 if (namesfile != "") {
185 read = new ReadOTUFile(globaldata->getListFile());
186 read->read(&*globaldata);
188 input = globaldata->ginput;
189 list = globaldata->gListVector;
190 string lastLabel = list->getLabel();
192 //if the users enters label "0.06" and there is no "0.06" in their file use the next lowest label.
193 set<string> processedLabels;
194 set<string> userLabels = labels;
197 while((list != NULL) && ((allLines == 1) || (userLabels.size() != 0))) {
199 if(allLines == 1 || labels.count(list->getLabel()) == 1){
201 error = process(list);
202 if (error == 1) { return 0; }
204 processedLabels.insert(list->getLabel());
205 userLabels.erase(list->getLabel());
208 if ((anyLabelsToProcess(list->getLabel(), userLabels, "") == true) && (processedLabels.count(lastLabel) != 1)) {
209 string saveLabel = list->getLabel();
212 list = input->getListVector(lastLabel);
214 error = process(list);
215 if (error == 1) { return 0; }
217 processedLabels.insert(list->getLabel());
218 userLabels.erase(list->getLabel());
220 //restore real lastlabel to save below
221 list->setLabel(saveLabel);
224 lastLabel = list->getLabel();
227 list = input->getListVector();
231 //output error messages about any remaining user labels
232 set<string>::iterator it;
233 bool needToRun = false;
234 for (it = userLabels.begin(); it != userLabels.end(); it++) {
235 mothurOut("Your file does not include the label " + *it);
236 if (processedLabels.count(lastLabel) != 1) {
237 mothurOut(". I will use " + lastLabel + "."); mothurOutEndLine();
240 mothurOut(". Please refer to " + lastLabel + "."); mothurOutEndLine();
244 //run last label if you need to
245 if (needToRun == true) {
246 if (list != NULL) { delete list; }
247 list = input->getListVector(lastLabel);
249 error = process(list);
250 if (error == 1) { return 0; }
257 catch(exception& e) {
258 errorOut(e, "BinSeqCommand", "execute");
263 //**********************************************************************************************************************
264 void BinSeqCommand::readNamesFile() {
266 vector<string> dupNames;
267 openInputFile(namesfile, inNames);
269 string name, names, sequence;
272 inNames >> name; //read from first column A
273 inNames >> names; //read from second column A,B,C,D
277 //parse names into vector
278 splitAtComma(names, dupNames);
280 //store names in fasta map
281 sequence = fasta->getSequence(name);
282 for (int i = 0; i < dupNames.size(); i++) {
283 fasta->push_back(dupNames[i], sequence);
291 catch(exception& e) {
292 errorOut(e, "BinSeqCommand", "readNamesFile");
296 //**********************************************************************************************************************
297 //return 1 if error, 0 otherwise
298 int BinSeqCommand::process(ListVector* list) {
300 string binnames, name, sequence;
302 string outputFileName = outputDir + getRootName(getSimpleName(globaldata->getListFile())) + list->getLabel() + ".fasta";
303 openOutputFile(outputFileName, out);
305 mothurOut(list->getLabel()); mothurOutEndLine();
307 //for each bin in the list vector
308 for (int i = 0; i < list->size(); i++) {
310 binnames = list->get(i);
311 while (binnames.find_first_of(',') != -1) {
312 name = binnames.substr(0,binnames.find_first_of(','));
313 binnames = binnames.substr(binnames.find_first_of(',')+1, binnames.length());
315 //do work for that name
316 sequence = fasta->getSequence(name);
317 if (sequence != "not found") {
318 //if you don't have groups
319 if (groupfile == "") {
320 name = name + "|" + toString(i+1);
321 out << ">" << name << endl;
322 out << sequence << endl;
323 }else {//if you do have groups
324 string group = groupMap->getGroup(name);
325 if (group == "not found") {
326 mothurOut(name + " is missing from your group file. Please correct. "); mothurOutEndLine();
327 remove(outputFileName.c_str());
330 name = name + "|" + group + "|" + toString(i+1);
331 out << ">" << name << endl;
332 out << sequence << endl;
336 mothurOut(name + " is missing from your fasta or name file. Please correct. "); mothurOutEndLine();
337 remove(outputFileName.c_str());
344 sequence = fasta->getSequence(binnames);
345 if (sequence != "not found") {
346 //if you don't have groups
347 if (groupfile == "") {
348 binnames = binnames + "|" + toString(i+1);
349 out << ">" << binnames << endl;
350 out << sequence << endl;
351 }else {//if you do have groups
352 string group = groupMap->getGroup(binnames);
353 if (group == "not found") {
354 mothurOut(binnames + " is missing from your group file. Please correct. "); mothurOutEndLine();
355 remove(outputFileName.c_str());
358 binnames = binnames + "|" + group + "|" + toString(i+1);
359 out << ">" << binnames << endl;
360 out << sequence << endl;
364 mothurOut(binnames + " is missing from your fasta or name file. Please correct. "); mothurOutEndLine();
365 remove(outputFileName.c_str());
374 catch(exception& e) {
375 errorOut(e, "BinSeqCommand", "process");
379 //**********************************************************************************************************************