added count file to get.sabund and get.rabund commands. fixed bug in windows paralellization of pairwise.seqs - cutoff was not passed to thread. added otulabels to shared file in remove.rare command. added check to make sure reorient is only used when paired barcodes are provided.
SarahsWork [Mon, 18 Mar 2013 17:36:59 +0000 (13:36 -0400)]
working on dereplicate=t issue in chimera.slayer and chimera.perseus, added appendFilesWithoutHeaders to mothurOut. fixed bug in make.contigs with headers. fixed name file overwrite in unique.seqs. fixed seq name mismatch in get.seqs if dups=f. added count file to seq. error command.
SarahsWork [Tue, 5 Mar 2013 19:13:00 +0000 (14:13 -0500)]
working on chimera.uchime change for dereplicate=t bug. added shared file to get.shareseqs command and changed groups parameters to shared groups and unique groups. fixed trimming bug with trim.flows. working on trim.seqs for paired barcodes.
SarahsWork [Wed, 27 Feb 2013 20:08:18 +0000 (15:08 -0500)]
mods to resolve some misc warnings. added alignPrimer function to needle man to allow for degenerated bases. added trimming of paired barcodes and primers to trim.seqs
Sarah Westcott [Tue, 12 Feb 2013 20:36:52 +0000 (15:36 -0500)]
added order=A or order=B to trim.flows and shhh.flows commands. added load balancing based on flow file size to shhh.flows. fixed bug in tree.shared subsampling. fixed bug in pcr.seqs with aligned files using both forward and reverse primers.
Sarah Westcott [Mon, 11 Feb 2013 12:14:38 +0000 (07:14 -0500)]
added ':' name check to seq. error and fastq.info. sffinfo now ignores clipQualRight values of 0. fixed bug in pcr.seqs if file was aligned and both forward and reverse primers were given, reverse primer was not trimmed properly. added pdiffs to pcr.seqs.
Sarah Westcott [Wed, 6 Feb 2013 16:44:10 +0000 (11:44 -0500)]
added *.count.summary file to count.groups command. added deltaq and insert parameters to make.contigs and changed contigs.report file. added illumina1.8+ format to fast commands. added contigsreport, align report and summary files to screen.seqs to allow files to be screened using their inputs. added minoverlap, ostart, oend, mismatches, maxn, minscore,maxinsert, minsim parameters to screen.seqs. added optimization types of minoverlap, ostart, oend, mismatches, maxn, minscore,maxinsert, minsim to screen.seqs. added getNumNs function to sequence class. working on Windows \\ issue.
Sarah Westcott [Tue, 29 Jan 2013 16:22:12 +0000 (11:22 -0500)]
added get.dists and remove.dists commands. fixed bug in trim.seqs windows paralellization that caused blank groups in group file. added list and shared file to get.otulabels and remove.otulabels. added list file to list.otulabels.
Sarah Westcott [Fri, 25 Jan 2013 20:46:33 +0000 (15:46 -0500)]
added primer.design command. fixed bug with linux unifrac subsampling, metastats output filename, venn command is no shared outs, added check for ':' in sequence names to avoid trouble with trees. added format parameter to make.contigs.
Sarah Westcott [Wed, 16 Jan 2013 16:30:18 +0000 (11:30 -0500)]
added topdown parameter to pre.cluster. added more debugging output to bayesian class. fixed mpi compile issue in cluster.split. fixed 32bit constant declarations in refchimeratest.cpp. fixed some commandName issues for gui interface
Sarah Westcott [Tue, 8 Jan 2013 17:19:41 +0000 (12:19 -0500)]
added dereplicate parameter to chimera.slayer and chimera.persues. added minnumsamples and minpercentsamples to filter.shared. fixed output names in cluster commands. added count parameter to parse.list. improved speed and memory requirements on sens.spec. added debugging code to some phylotree functions.
Sarah Westcott [Tue, 30 Oct 2012 18:04:45 +0000 (14:04 -0400)]
classify.seqs allows sequences to be in taxonomy file that are not in template. it ignores them. fixed windows bug in clearcut char* declarations. get.lineage and remove.lineage can now handle taxons with (). list vector sorts list file outs by abundance. bugs while testing
Sarah Westcott [Mon, 22 Oct 2012 15:40:41 +0000 (11:40 -0400)]
added sets to amova and homova commands. added oligos to make.contigs. added metadata to make.biom. fixed bug in get.oturep caused by adding the count file. changed vector declaration in cooccurrence to fixed windows issue. classify.seqs can now ignore seqs in taxonomy file not in template file.
Sarah Westcott [Tue, 2 Oct 2012 18:36:18 +0000 (14:36 -0400)]
added classify.shared command and random forest files. added count file to pcr.seqs, remove.rare, screen.seqs, sort.seqs, split.abund, and subsample commands
Sarah Westcott [Tue, 25 Sep 2012 13:18:08 +0000 (09:18 -0400)]
added count file to get.oturep, pre.cluster, screen.seqs, tree.shared. made remove.lineage and get.lineage more flexible to remove or get taxons wrapped in quotes if not given in taxon parameter.
Sarah Westcott [Fri, 7 Sep 2012 18:30:06 +0000 (14:30 -0400)]
major change to the tree class to use the count table class instead of tree map. This change involved changes to subsample class for trees, changes to the tree reader and readTree class. This change fixed bug with parsimony random tree generation that was leaving out the name file info. added count file to unifrac.unweighted, unifrac.weighted, parsimony, bin.seqs, classify.otu, classify.seqs, classify.tree, cluster.fragments, consensus.seqs, unique.seqs, phylo.diversity, summary.tax.
Sarah Westcott [Tue, 21 Aug 2012 17:39:22 +0000 (13:39 -0400)]
added oligos, pdiffs, bdiffs, ldiffs, sdiffs, tiffs parameters to sffinfo to allow for spitting the sff file by barcode. working on sff.multiple command.