if (abort == true) { if (calledHelp) { return 0; } return 2; }
int start = time(NULL);
- fileAligned = true;
+ fileAligned = true; pairedOligos = false;
string thisOutputDir = outputDir;
if (outputDir == "") { thisOutputDir += m->hasPath(fastafile); }
length = 0;
- if(oligosfile != ""){ readOligos(); if (m->debug) { m->mothurOut("[DEBUG]: read oligos file. numprimers = " + toString(primers.size()) + ", revprimers = " + toString(revPrimer.size()) + ".\n"); } } if (m->control_pressed) { return 0; }
+ if(oligosfile != ""){ readOligos(); if (m->debug) { m->mothurOut("[DEBUG]: read oligos file. numprimers = " + toString(numFPrimers) + ", revprimers = " + toString(numRPrimers) + ".\n"); } } if (m->control_pressed) { return 0; }
if(ecolifile != "") { readEcoli(); } if (m->control_pressed) { return 0; }
vector<unsigned long long> positions;
//loop through and create all the processes you want
while (process != processors) {
- int pid = fork();
+ pid_t pid = fork();
if (pid > 0) {
processIDS.push_back(pid); //create map from line number to pid so you can append files in correct order later
process++;
}else if (pid == 0){
- string locationsFile = toString(getpid()) + ".temp";
- num = driverPcr(filename, goodFileName + toString(getpid()) + ".temp", badFileName + toString(getpid()) + ".temp", locationsFile, badSeqNames, lines[process], pstart, adjustNeeded);
+ string locationsFile = m->mothurGetpid(process) + ".temp";
+ num = driverPcr(filename, goodFileName + m->mothurGetpid(process) + ".temp", badFileName + m->mothurGetpid(process) + ".temp", locationsFile, badSeqNames, lines[process], pstart, adjustNeeded);
//pass numSeqs to parent
ofstream out;
- string tempFile = filename + toString(getpid()) + ".num.temp";
+ string tempFile = filename + m->mothurGetpid(process) + ".num.temp";
m->openOutputFile(tempFile, out);
out << pstart << '\t' << adjustNeeded << endl;
out << num << '\t' << badSeqNames.size() << endl;
}
}
- string locationsFile = toString(getpid()) + ".temp";
+ string locationsFile = m->mothurGetpid(process) + ".temp";
num = driverPcr(filename, goodFileName, badFileName, locationsFile, badSeqNames, lines[0], pstart, adjustNeeded);
//force parent to wait until all the processes are done
if (i!=0) {extension += toString(i) + ".temp"; processIDS.push_back(i); }
// Allocate memory for thread data.
- pcrData* tempPcr = new pcrData(filename, goodFileName+extension, badFileName+extension, locationsFile+extension, m, oligosfile, ecolifile, primers, revPrimer, nomatch, keepprimer, keepdots, start, end, length, pdiffs, lines[i].start, lines[i].end);
+ pcrData* tempPcr = new pcrData(filename, goodFileName+extension, badFileName+extension, locationsFile+extension, m, oligosfile, ecolifile, nomatch, keepprimer, keepdots, start, end, length, pdiffs, lines[i].start, lines[i].end);
pDataArray.push_back(tempPcr);
//default security attributes, thread function name, argument to thread function, use default creation flags, returns the thread identifier
- if (fileAligned) {
+ if (fileAligned && adjustNeeded) {
//find pend - pend is the biggest ending value, but we must account for when we adjust the start. That adjustment may make the "new" end larger then the largest end. So lets find out what that "new" end will be.
ifstream inLocations;
m->openInputFile(locationsFile, inLocations);
string name = "";
int thisStart = -1; int thisEnd = -1;
- if (primers.size() != 0) { inLocations >> name >> thisStart; m->gobble(inLocations); }
- if (revPrimer.size() != 0) { inLocations >> name >> thisEnd; m->gobble(inLocations); }
+ if (numFPrimers != 0) { inLocations >> name >> thisStart; m->gobble(inLocations); }
+ if (numRPrimers != 0) { inLocations >> name >> thisEnd; m->gobble(inLocations); }
else { pend = -1; break; }
int myDiff = 0;
inLocations.close();
adjustDots(goodFileName, locationsFile, pstart, pend);
- }
+ }else { m->mothurRemove(locationsFile); }
return num;
set<int> locations; //locations[0] = beginning locations,
//pdiffs, bdiffs, primers, barcodes, revPrimers
- map<string, int> faked;
- TrimOligos trim(pdiffs, 0, primers, faked, revPrimer);
+ map<string, int> primers;
+ map<string, int> barcodes; //not used
+ vector<string> revPrimer;
+ if (pairedOligos) {
+ map<int, oligosPair> primerPairs = oligos.getPairedPrimers();
+ for (map<int, oligosPair>::iterator it = primerPairs.begin(); it != primerPairs.end(); it++) {
+ primers[(it->second).forward] = it->first;
+ revPrimer.push_back((it->second).reverse);
+ }
+ if (pdiffs != 0) { m->mothurOut("[WARNING]: Pcr.seqs is only designed to allow diffs in forward primers. Reverse primers must be an exact match.\n"); }
+ }else{
+ primers = oligos.getPrimers();
+ revPrimer = oligos.getReversePrimers();
+ }
+
+ TrimOligos trim(pdiffs, 0, primers, barcodes, revPrimer);
while (!done) {
string name = "";
int thisStart = -1; int thisEnd = -1;
- if (primers.size() != 0) { inLocations >> name >> thisStart; m->gobble(inLocations); }
- if (revPrimer.size() != 0) { inLocations >> name >> thisEnd; m->gobble(inLocations); }
+ if (numFPrimers != 0) { inLocations >> name >> thisStart; m->gobble(inLocations); }
+ if (numRPrimers != 0) { inLocations >> name >> thisEnd; m->gobble(inLocations); }
//cout << seq.getName() << '\t' << thisStart << '\t' << thisEnd << '\t' << seq.getAligned().length() << endl;
exit(1);
}
}
-//********************************************************************/
-string PcrSeqsCommand::reverseOligo(string oligo){
- try {
- string reverse = "";
-
- for(int i=oligo.length()-1;i>=0;i--){
-
- if(oligo[i] == 'A') { reverse += 'T'; }
- else if(oligo[i] == 'T'){ reverse += 'A'; }
- else if(oligo[i] == 'U'){ reverse += 'A'; }
-
- else if(oligo[i] == 'G'){ reverse += 'C'; }
- else if(oligo[i] == 'C'){ reverse += 'G'; }
-
- else if(oligo[i] == 'R'){ reverse += 'Y'; }
- else if(oligo[i] == 'Y'){ reverse += 'R'; }
-
- else if(oligo[i] == 'M'){ reverse += 'K'; }
- else if(oligo[i] == 'K'){ reverse += 'M'; }
-
- else if(oligo[i] == 'W'){ reverse += 'W'; }
- else if(oligo[i] == 'S'){ reverse += 'S'; }
-
- else if(oligo[i] == 'B'){ reverse += 'V'; }
- else if(oligo[i] == 'V'){ reverse += 'B'; }
-
- else if(oligo[i] == 'D'){ reverse += 'H'; }
- else if(oligo[i] == 'H'){ reverse += 'D'; }
-
- else { reverse += 'N'; }
- }
-
-
- return reverse;
- }
- catch(exception& e) {
- m->errorOut(e, "PcrSeqsCommand", "reverseOligo");
- exit(1);
- }
-}
-
-//***************************************************************************************************************
-bool PcrSeqsCommand::readOligos(){
- try {
- ifstream inOligos;
- m->openInputFile(oligosfile, inOligos);
-
- string type, oligo, group;
- int primerCount = 0;
-
- while(!inOligos.eof()){
-
- inOligos >> type;
-
- if(type[0] == '#'){ //ignore
- while (!inOligos.eof()) { char c = inOligos.get(); if (c == 10 || c == 13){ break; } } // get rest of line if there's any crap there
- m->gobble(inOligos);
- }else{
- m->gobble(inOligos);
- //make type case insensitive
- for(int i=0;i<type.length();i++){ type[i] = toupper(type[i]); }
-
- inOligos >> oligo;
-
- for(int i=0;i<oligo.length();i++){
- oligo[i] = toupper(oligo[i]);
- if(oligo[i] == 'U') { oligo[i] = 'T'; }
- }
-
- if(type == "FORWARD"){
- // get rest of line in case there is a primer name
- while (!inOligos.eof()) {
- char c = inOligos.get();
- if (c == 10 || c == 13 || c == -1){ break; }
- else if (c == 32 || c == 9){;} //space or tab
- }
- primers[oligo] = primerCount; primerCount++;
- //cout << "for oligo = " << oligo << endl;
- }else if(type == "REVERSE"){
- string oligoRC = reverseOligo(oligo);
- revPrimer.push_back(oligoRC);
- //cout << "rev oligo = " << oligo << " reverse = " << oligoRC << endl;
- }else if(type == "BARCODE"){
- inOligos >> group;
- }else if(type == "PRIMER"){
- m->gobble(inOligos);
- primers[oligo] = primerCount; primerCount++;
-
- string roligo="";
- inOligos >> roligo;
-
- for(int i=0;i<roligo.length();i++){
- roligo[i] = toupper(roligo[i]);
- if(roligo[i] == 'U') { roligo[i] = 'T'; }
- }
- revPrimer.push_back(reverseOligo(roligo));
-
- // get rest of line in case there is a primer name
- while (!inOligos.eof()) {
- char c = inOligos.get();
- if (c == 10 || c == 13 || c == -1){ break; }
- else if (c == 32 || c == 9){;} //space or tab
- }
- //cout << "prim oligo = " << oligo << " reverse = " << roligo << endl;
- }else if((type == "LINKER")||(type == "SPACER")) {;}
- else{ m->mothurOut(type + " is not recognized as a valid type. Choices are primer, forward, reverse, linker, spacer and barcode. Ignoring " + oligo + "."); m->mothurOutEndLine(); m->control_pressed = true; }
- }
- m->gobble(inOligos);
- }
- inOligos.close();
-
- if ((primers.size() == 0) && (revPrimer.size() == 0)) {
- m->mothurOut("[ERROR]: your oligos file does not contain valid primers or reverse primers. Please correct."); m->mothurOutEndLine();
- m->control_pressed = true;
- return false;
- }
-
- return true;
-
- }catch(exception& e) {
- m->errorOut(e, "PcrSeqsCommand", "readOligos");
- exit(1);
- }
-}
//***************************************************************************************************************
bool PcrSeqsCommand::readEcoli(){
try {
exit(1);
}
}
+//***************************************************************************************************************
+
+int PcrSeqsCommand::readOligos(){
+ try {
+ oligos.read(oligosfile);
+
+ if (m->control_pressed) { return false; } //error in reading oligos
+
+ if (oligos.hasPairedBarcodes()) {
+ pairedOligos = true;
+ numFPrimers = oligos.getPairedPrimers().size();
+ }else {
+ pairedOligos = false;
+ numFPrimers = oligos.getPrimers().size();
+ }
+ numRPrimers = oligos.getReversePrimers().size();
+
+ if (oligos.getLinkers().size() != 0) { m->mothurOut("[WARNING]: pcr.seqs is not setup to remove linkers, ignoring.\n"); }
+ if (oligos.getSpacers().size() != 0) { m->mothurOut("[WARNING]: pcr.seqs is not setup to remove spacers, ignoring.\n"); }
+
+ return true;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "PcrSeqsCommand", "readOligos");
+ exit(1);
+ }
+}
+
/**************************************************************************************/
projects / mothur.git / blobdiff