#include "makebiomcommand.h"
#include "sharedrabundvector.h"
#include "inputdata.h"
+#include "sharedutilities.h"
//taken from http://biom-format.org/documentation/biom_format.html
/* Minimal Sparse
try {
CommandParameter pshared("shared", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(pshared);
CommandParameter pcontaxonomy("contaxonomy", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(pcontaxonomy);
+ CommandParameter pmetadata("metadata", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(pmetadata);
CommandParameter pgroups("groups", "String", "", "", "", "", "",false,false); parameters.push_back(pgroups);
CommandParameter plabel("label", "String", "", "", "", "", "",false,false); parameters.push_back(plabel);
CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
string MakeBiomCommand::getHelpString(){
try {
string helpString = "";
- helpString += "The make.biom command parameters are shared, contaxonomy, groups, matrixtype and label. shared is required, unless you have a valid current file.\n";
+ helpString += "The make.biom command parameters are shared, contaxonomy, metadata, groups, matrixtype and label. shared is required, unless you have a valid current file.\n";
helpString += "The groups parameter allows you to specify which of the groups in your groupfile you would like included. The group names are separated by dashes.\n";
helpString += "The label parameter allows you to select what distance levels you would like, and are also separated by dashes.\n";
helpString += "The matrixtype parameter allows you to select what type you would like to make. Choices are sparse and dense, default is sparse.\n";
- helpString += "The contaxonomy file is the taxonomy file outputted by classify.otu(list=yourListfile, taxonomy=yourTaxonomyFile). Be SURE that the you are the constaxonomy file distance matches the shared file distance. ie, for *.0.03.cons.taxonomy set label=0.03. Mothur is smart enough to handle shared files that have been subsampled.\n";
+ helpString += "The contaxonomy file is the taxonomy file outputted by classify.otu(list=yourListfile, taxonomy=yourTaxonomyFile). Be SURE that the you are the constaxonomy file distance matches the shared file distance. ie, for *.0.03.cons.taxonomy set label=0.03. Mothur is smart enough to handle shared files that have been subsampled. It is used to assign taxonomy information to the metadata of rows.\n";
+ helpString += "The metadata parameter is used to provide experimental parameters to the columns. Things like 'sample1 gut human_gut'. \n";
helpString += "The make.biom command should be in the following format: make.biom(shared=yourShared, groups=yourGroups, label=yourLabels).\n";
helpString += "Example make.biom(shared=abrecovery.an.shared, groups=A-B-C).\n";
helpString += "The default value for groups is all the groups in your groupfile, and all labels in your inputfile will be used.\n";
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["contaxonomy"] = inputDir + it->second; }
}
+
+ it = parameters.find("metadata");
+ //user has given a template file
+ if(it != parameters.end()){
+ path = m->hasPath(it->second);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { parameters["metadata"] = inputDir + it->second; }
+ }
}
//get shared file
if (contaxonomyfile == "not found") { contaxonomyfile = ""; }
else if (contaxonomyfile == "not open") { contaxonomyfile = ""; abort = true; }
+ metadatafile = validParameter.validFile(parameters, "metadata", true);
+ if (metadatafile == "not found") { metadatafile = ""; }
+ else if (metadatafile == "not open") { metadatafile = ""; abort = true; }
//check for optional parameter and set defaults
// ...at some point should added some additional type checking...
labels.insert(lastLabel);
}
+ getSampleMetaData(lookup);
+
//if the users enters label "0.06" and there is no "0.06" in their file use the next lowest label.
set<string> processedLabels;
set<string> userLabels = labels;
{"id":"Sample6", "metadata":null}
],*/
- string colBack = "\", \"metadata\":null}";
+ string colBack = "\", \"metadata\":";
out << spaces + "\"columns\":[\n";
for (int i = 0; i < lookup.size()-1; i++) {
if (m->control_pressed) { out.close(); return 0; }
- out << rowFront << lookup[i]->getGroup() << colBack << ",\n";
+ out << rowFront << lookup[i]->getGroup() << colBack << sampleMetadata[i] << "},\n";
}
- out << rowFront << lookup[(lookup.size()-1)]->getGroup() << colBack << "\n" + spaces + "],\n";
+ out << rowFront << lookup[(lookup.size()-1)]->getGroup() << colBack << sampleMetadata[lookup.size()-1] << "}\n" + spaces + "],\n";
out << spaces + "\"matrix_type\": \"" << format << "\",\n" + spaces + "\"matrix_element_type\": \"int\",\n";
out << spaces + "\"shape\": [" << m->currentBinLabels.size() << "," << lookup.size() << "],\n";
}
}
+//**********************************************************************************************************************
+int MakeBiomCommand::getSampleMetaData(vector<SharedRAbundVector*>& lookup){
+ try {
+
+ if (metadatafile == "") { for (int i = 0; i < lookup.size(); i++) { sampleMetadata.push_back("null"); } }
+ else {
+ ifstream in;
+ m->openInputFile(metadatafile, in);
+
+ vector<string> groupNames, metadataLabels;
+ map<string, vector<string> > lines;
+
+ string headerLine = m->getline(in); m->gobble(in);
+ vector<string> pieces = m->splitWhiteSpace(headerLine);
+
+ //save names of columns you are reading
+ for (int i = 1; i < pieces.size(); i++) {
+ metadataLabels.push_back(pieces[i]);
+ }
+ int count = metadataLabels.size();
+
+ vector<string> groups = m->getGroups();
+
+ //read rest of file
+ while (!in.eof()) {
+
+ if (m->control_pressed) { in.close(); return 0; }
+
+ string group = "";
+ in >> group; m->gobble(in);
+ groupNames.push_back(group);
+
+ string line = m->getline(in); m->gobble(in);
+ vector<string> thisPieces = m->splitWhiteSpaceWithQuotes(line);
+
+ if (thisPieces.size() != count) { m->mothurOut("[ERROR]: expected " + toString(count) + " items of data for sample " + group + " read " + toString(thisPieces.size()) + ", quitting.\n"); }
+ else { if (m->inUsersGroups(group, groups)) { lines[group] = thisPieces; } }
+
+ m->gobble(in);
+ }
+ in.close();
+
+ map<string, vector<string> >::iterator it;
+ for (int i = 0; i < lookup.size(); i++) {
+
+ if (m->control_pressed) { return 0; }
+
+ it = lines.find(lookup[i]->getGroup());
+
+ if (it == lines.end()) { m->mothurOut("[ERROR]: can't find metadata information for " + lookup[i]->getGroup() + ", quitting.\n"); m->control_pressed = true; }
+ else {
+ vector<string> values = it->second;
+
+ string data = "{";
+ for (int j = 0; j < metadataLabels.size()-1; j++) {
+ values[j] = m->removeQuotes(values[j]);
+ data += "\"" + metadataLabels[j] + "\":\"" + values[j] + "\", ";
+ }
+ values[metadataLabels.size()-1] = m->removeQuotes(values[metadataLabels.size()-1]);
+ data += "\"" + metadataLabels[metadataLabels.size()-1] + "\":\"" + values[metadataLabels.size()-1] + "\"}";
+ sampleMetadata.push_back(data);
+ }
+ }
+ }
+
+ return 0;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "MakeBiomCommand", "getSampleMetaData");
+ exit(1);
+ }
+
+}
+
/**************************************************************************************************/
//returns {Bacteria, Bacteroidetes, ..} and scores is filled with {100, 98, ...} or {null, null, null}
vector<string> MakeBiomCommand::parseTax(string tax, vector<string>& scores) {
projects / mothur.git / blobdiff