*/
#include "decalc.h"
+#include "chimera.h"
+#include "dist.h"
+#include "eachgapdist.h"
+#include "ignoregaps.h"
+
//***************************************************************************************************************
-void DeCalculator::setMask(string m) {
+void DeCalculator::setMask(string ms) {
try {
- seqMask = m;
+ seqMask = ms;
+ int count = 0;
+ maskMap.clear();
if (seqMask.length() != 0) {
//whereever there is a base in the mask, save that value is query and subject
for (int i = 0; i < seqMask.length(); i++) {
if (isalpha(seqMask[i])) {
h.insert(i);
+ maskMap[count] = i;
+ count++;
+
}
}
}else {
- for (int i = 0; i < alignLength; i++) { h.insert(i); }
+ for (int i = 0; i < alignLength; i++) {
+ h.insert(i);
+ maskMap[count] = i;
+ count++;
+ }
}
}
catch(exception& e) {
- errorOut(e, "DeCalculator", "setMask");
+ m->errorOut(e, "DeCalculator", "setMask");
exit(1);
}
}
seq->setUnaligned(tempQuery);
}
catch(exception& e) {
- errorOut(e, "DeCalculator", "runMask");
+ m->errorOut(e, "DeCalculator", "runMask");
exit(1);
}
}
}
catch(exception& e) {
- errorOut(e, "DeCalculator", "trimSeqs");
+ m->errorOut(e, "DeCalculator", "trimSeqs");
exit(1);
}
}
//***************************************************************************************************************
-//find the window breaks for each sequence - this is so you can move ahead by bases.
vector<int> DeCalculator::findWindows(Sequence* query, int front, int back, int& size, int increment) {
try {
if (size == 0) { if (cutoff > 1200) { size = 300; }
else{ size = (cutoff / 4); } }
else if (size > (cutoff / 4)) {
- mothurOut("You have selected to large a window size for sequence " + query->getName() + ". I will choose an appropriate window size."); mothurOutEndLine();
+ m->mothurOut("You have selected too large a window size for sequence " + query->getName() + ". I will choose an appropriate window size."); m->mothurOutEndLine();
size = (cutoff / 4);
}
}
catch(exception& e) {
- errorOut(e, "DeCalculator", "findWindows");
+ m->errorOut(e, "DeCalculator", "findWindows");
exit(1);
}
}
vector<float> DeCalculator::calcObserved(Sequence* query, Sequence* subject, vector<int> window, int size) {
try {
- vector<float> temp;
-//cout << "query length = " << query->getAligned().length() << '\t' << " subject length = " << subject.getAligned().length() << endl;
+ vector<float> temp;
+ //int gaps = 0;
for (int m = 0; m < window.size(); m++) {
string seqFrag = query->getAligned().substr(window[m], size);
string seqFragsub = subject->getAligned().substr(window[m], size);
- //cout << "start point = " << window[m] << " end point = " << window[m]+size << endl;
+
int diff = 0;
for (int b = 0; b < seqFrag.length(); b++) {
-
- if (seqFrag[b] != seqFragsub[b]) { diff++; }
+ //if at least one is a base and they are not equal
+ if( (isalpha(seqFrag[b]) || isalpha(seqFragsub[b])) && (seqFrag[b] != seqFragsub[b]) ) { diff++; }
+
+ //ignore gaps
+ //if((!isalpha(seqFrag[b])) && (!isalpha(seqFragsub[b]))) { gaps++; }
}
//percentage of mismatched bases
float dist;
- dist = diff / (float) seqFrag.length() * 100;
-
+
+ //if the whole fragment is 0 distance = 0
+ //if ((seqFrag.length()-gaps) == 0) { dist = 0.0; }
+
+ //percentage of mismatched bases
+ //else { dist = diff / (float) (seqFrag.length()-gaps) * 100; }
+
+ dist = diff / (float) (seqFrag.length()) * 100;
+
temp.push_back(dist);
}
return temp;
}
catch(exception& e) {
- errorOut(e, "DeCalculator", "calcObserved");
+ m->errorOut(e, "DeCalculator", "calcObserved");
exit(1);
}
}
if((!isalpha(seqFrag[b])) && (!isalpha(seqFragsub[b]))) { gaps++; }
if (seqFrag[b] != seqFragsub[b]) { diff++; }
}
+
+ //if the whole fragment is 0 distance = 0
+ if ((seqFrag.length()-gaps) == 0) { return 0.0; }
//percentage of mismatched bases
float dist = diff / (float) (seqFrag.length()-gaps) * 100;
return dist;
}
catch(exception& e) {
- errorOut(e, "DeCalculator", "calcDist");
+ m->errorOut(e, "DeCalculator", "calcDist");
exit(1);
}
}
}
catch(exception& e) {
- errorOut(e, "DeCalculator", "calcExpected");
+ m->errorOut(e, "DeCalculator", "calcExpected");
exit(1);
}
}
//for each window
float sum = 0.0; //sum = sum from 1 to m of (oi-ei)^2
- for (int m = 0; m < obs.size(); m++) { sum += ((obs[m] - exp[m]) * (obs[m] - exp[m])); }
+ int numZeros = 0;
+ for (int m = 0; m < obs.size(); m++) {
+
+ //if (obs[m] != 0.0) {
+ sum += ((obs[m] - exp[m]) * (obs[m] - exp[m]));
+ //}else { numZeros++; }
+
+ }
- float de = sqrt((sum / (obs.size() - 1)));
+ float de = sqrt((sum / (obs.size() - 1 - numZeros)));
return de;
}
catch(exception& e) {
- errorOut(e, "DeCalculator", "calcDE");
+ m->errorOut(e, "DeCalculator", "calcDE");
exit(1);
}
}
}
catch(exception& e) {
- errorOut(e, "DeCalculator", "calcFreq");
+ m->errorOut(e, "DeCalculator", "calcFreq");
exit(1);
}
}
return averages;
}
catch(exception& e) {
- errorOut(e, "DeCalculator", "findQav");
+ m->errorOut(e, "DeCalculator", "findQav");
exit(1);
}
}
-
//***************************************************************************************************************
-vector< vector<float> > DeCalculator::getQuantiles(vector<Sequence*> seqs, vector<int> windowSizesTemplate, int window, vector<float> probProfile, int increment, int start, int end) {
+//seqs have already been masked
+vector< vector<quanMember> > DeCalculator::getQuantiles(vector<Sequence*> seqs, vector<int> windowSizesTemplate, int window, vector<float> probProfile, int increment, int start, int end) {
try {
- vector< vector<float> > quan;
+ vector< vector<quanMember> > quan;
//percentage of mismatched pairs 1 to 100
quan.resize(100);
-
+//ofstream o;
+//string out = "getQuantiles.out";
+//openOutputFile(out, o);
//for each sequence
for(int i = start; i < end; i++){
- mothurOut("Processing template sequence " + toString(i)); mothurOutEndLine();
- Sequence* query = seqs[i];
+ m->mothurOut("Processing sequence " + toString(i)); m->mothurOutEndLine();
+ Sequence* query = new Sequence(seqs[i]->getName(), seqs[i]->getAligned());
//compare to every other sequence in template
for(int j = 0; j < i; j++){
- Sequence* subject = seqs[j];
+ Sequence* subject = new Sequence(seqs[j]->getName(), seqs[j]->getAligned());
map<int, int> trim;
map<int, int>::iterator it;
float de = calcDE(obsi, exp);
float dist = calcDist(query, subject, front, back);
-
+ //o << i << '\t' << j << '\t' << dist << '\t' << de << endl;
dist = ceil(dist);
- //dist-1 because vector indexes start at 0.
- quan[dist-1].push_back(de);
+ quanMember newScore(de, i, j);
+
+ quan[dist].push_back(newScore);
+ delete subject;
}
+
+ delete query;
}
return quan;
}
catch(exception& e) {
- errorOut(e, "DeCalculator", "findQav");
+ m->errorOut(e, "DeCalculator", "getQuantiles");
exit(1);
}
}
+//********************************************************************************************************************
+//sorts lowest to highest
+inline bool compareQuanMembers(quanMember left, quanMember right){
+ return (left.score < right.score);
+}
//***************************************************************************************************************
-void DeCalculator::removeObviousOutliers(vector< vector<float> >& quantiles) {
+//this was going to be used by pintail to increase the sensitivity of the chimera detection, but it wasn't quite right. may want to revisit in the future...
+void DeCalculator::removeObviousOutliers(vector< vector<quanMember> >& quantiles, int num) {
try {
-
-
+
for (int i = 0; i < quantiles.size(); i++) {
//find mean of this quantile score
- sort(quantiles[i].begin(), quantiles[i].end());
+ sort(quantiles[i].begin(), quantiles[i].end(), compareQuanMembers);
+
+ float high = quantiles[i][int(quantiles[i].size() * 0.99)].score;
+ float low = quantiles[i][int(quantiles[i].size() * 0.01)].score;
+
+ vector<quanMember> temp;
- float average = quantiles[i][int(quantiles[i].size() * 0.5)];
-cout << i << "\taverage = " << average << "\tquantiles[i].size = " << quantiles[i].size() << endl;
- vector<float> newQuanI;
//look at each value in quantiles to see if it is an outlier
for (int j = 0; j < quantiles[i].size(); j++) {
-
- float highCutOff, lowCutOff;
-
- //99%
- highCutOff = sqrt(((quantiles[i][j] - average + 3) * (quantiles[i][j] - average + 3)) / (float)(quantiles[i].size() - 1));
-
- //1%
- lowCutOff = sqrt(((quantiles[i][j] - average - 3) * (quantiles[i][j] - average + 3)) / (float)(quantiles[i].size() - 1));
-//cout << "high = " << highCutOff << " low = " << lowCutOff << " de = " << quantiles[i][j] << endl;
- //if this is below the highcutff and above the lowcutoff
- if ((quantiles[i][j] < highCutOff) && (quantiles[i][j] > lowCutOff)) {
-
- newQuanI.push_back(quantiles[i][j]);
-
- }else { cout << "removed outlier: high = " << highCutOff << " low = " << lowCutOff << " de = " << quantiles[i][j] << endl; }
+ //is this score between 1 and 99%
+ if ((quantiles[i][j].score > low) && (quantiles[i][j].score < high)) {
+ temp.push_back(quantiles[i][j]);
+ }
}
- quantiles[i] = newQuanI;
+ quantiles[i] = temp;
+ }
+
+/*
+ //find contributer with most offending score related to it
+ int largestContrib = findLargestContrib(seen);
+
+ //while you still have guys to eliminate
+ while (contributions.size() > 0) {
+ m->mothurOut("Removing scores contributed by sequence " + toString(largestContrib) + " in your template file."); m->mothurOutEndLine();
+
+ //remove from quantiles all scores that were made using this largestContrib
+ for (int i = 0; i < quantiles.size(); i++) {
+//cout << "before remove " << quantiles[i].size() << '\t';
+ removeContrib(largestContrib, quantiles[i]);
+//cout << "after remove " << quantiles[i].size() << endl;
+ }
+//cout << count << " largest contrib = " << largestContrib << endl; count++;
+ //remove from contributions all scores that were made using this largestContrib
+ removeContrib(largestContrib, contributions);
+
+ //"erase" largestContrib
+ seen[largestContrib] = -1;
+
+ //get next largestContrib
+ largestContrib = findLargestContrib(seen);
}
+ABOVE IS ATTEMPT #1
+**************************************************************************************************
+BELOW IS ATTEMPT #2
+ vector<int> marked = returnObviousOutliers(quantiles, num);
+ vector<int> copyMarked = marked;
+
+ //find the 99% of marked
+ sort(copyMarked.begin(), copyMarked.end());
+ int high = copyMarked[int(copyMarked.size() * 0.99)];
+cout << "high = " << high << endl;
+
+ for(int i = 0; i < marked.size(); i++) {
+ if (marked[i] > high) {
+ m->mothurOut("Removing scores contributed by sequence " + toString(marked[i]) + " in your template file."); m->mothurOutEndLine();
+ for (int i = 0; i < quantiles.size(); i++) {
+ removeContrib(marked[i], quantiles[i]);
+ }
+ }
+ }
+
+
+ //adjust quantiles
+ for (int i = 0; i < quantiles.size(); i++) {
+ vector<float> temp;
+
+ if (quantiles[i].size() == 0) {
+ //in case this is not a distance found in your template files
+ for (int g = 0; g < 6; g++) {
+ temp.push_back(0.0);
+ }
+ }else{
+
+ sort(quantiles[i].begin(), quantiles[i].end(), compareQuanMembers);
+
+ //save 10%
+ temp.push_back(quantiles[i][int(quantiles[i].size() * 0.10)].score);
+ //save 25%
+ temp.push_back(quantiles[i][int(quantiles[i].size() * 0.25)].score);
+ //save 50%
+ temp.push_back(quantiles[i][int(quantiles[i].size() * 0.5)].score);
+ //save 75%
+ temp.push_back(quantiles[i][int(quantiles[i].size() * 0.75)].score);
+ //save 95%
+ temp.push_back(quantiles[i][int(quantiles[i].size() * 0.95)].score);
+ //save 99%
+ temp.push_back(quantiles[i][int(quantiles[i].size() * 0.99)].score);
+
+ }
+
+ quan[i] = temp;
+
+ }
+*/
+
}
catch(exception& e) {
- errorOut(e, "DeCalculator", "removeObviousOutliers");
+ m->errorOut(e, "DeCalculator", "removeObviousOutliers");
+ exit(1);
+ }
+}
+//***************************************************************************************************************
+//put quanMember in the vector based on how far they are from the 99% or 1%. Biggest offenders in front.
+/*vector<quanMember> DeCalculator::sortContrib(map<quanMember*, float> quan) {
+ try{
+
+ vector<quanMember> newQuan;
+ map<quanMember*, float>::iterator it;
+
+ while (quan.size() > 0) {
+
+ map<quanMember*, float>::iterator largest = quan.begin();
+
+ //find biggest member
+ for (it = quan.begin(); it != quan.end(); it++) {
+ if (it->second > largest->second) { largest = it; }
+ }
+cout << largest->second << '\t' << largest->first->score << '\t' << largest->first->member1 << '\t' << largest->first->member2 << endl;
+ //save it
+ newQuan.push_back(*(largest->first));
+
+ //erase from quan
+ quan.erase(largest);
+ }
+
+ return newQuan;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "DeCalculator", "sortContrib");
exit(1);
}
}
+//***************************************************************************************************************
+//used by removeObviousOutliers which was attempt to increase sensitivity of chimera detection...not currently used...
+int DeCalculator::findLargestContrib(vector<int> seen) {
+ try{
+
+ int largest = 0;
+
+ int largestContribs;
+
+ for (int i = 0; i < seen.size(); i++) {
+
+ if (seen[i] > largest) {
+ largestContribs = i;
+ largest = seen[i];
+ }
+ }
+
+ return largestContribs;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "DeCalculator", "findLargestContribs");
+ exit(1);
+ }
+}
+//***************************************************************************************************************
+void DeCalculator::removeContrib(int bad, vector<quanMember>& quan) {
+ try{
+
+ vector<quanMember> newQuan;
+ for (int i = 0; i < quan.size(); i++) {
+ if ((quan[i].member1 != bad) && (quan[i].member2 != bad) ) {
+ newQuan.push_back(quan[i]);
+ }
+ }
+
+ quan = newQuan;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "DeCalculator", "removeContrib");
+ exit(1);
+ }
+}
+*/
//***************************************************************************************************************
float DeCalculator::findAverage(vector<float> myVector) {
try{
}
catch(exception& e) {
- errorOut(e, "DeCalculator", "findAverage");
+ m->errorOut(e, "DeCalculator", "findAverage");
exit(1);
}
}
return coef;
}
catch(exception& e) {
- errorOut(e, "DeCalculator", "getCoef");
+ m->errorOut(e, "DeCalculator", "getCoef");
exit(1);
}
}
//***************************************************************************************************************
+//gets closest matches to each end, since chimeras will most likely have different parents on each end
+vector<Sequence*> DeCalculator::findClosest(Sequence* querySeq, vector<Sequence*> db, int& numWanted, vector<int>& indexes) {
+ try {
+ indexes.clear();
+
+ vector<Sequence*> seqsMatches;
+ vector<SeqDist> distsLeft;
+ vector<SeqDist> distsRight;
+
+ Dist* distcalculator = new eachGapDist();
+
+ string queryUnAligned = querySeq->getUnaligned();
+ int numBases = int(queryUnAligned.length() * 0.33);
+
+ string leftQuery = ""; //first 1/3 of the sequence
+ string rightQuery = ""; //last 1/3 of the sequence
+ string queryAligned = querySeq->getAligned();
+
+ //left side
+ bool foundFirstBase = false;
+ int baseCount = 0;
+ int leftSpot = 0;
+ int firstBaseSpot = 0;
+ for (int i = 0; i < queryAligned.length(); i++) {
+ //if you are a base
+ if (isalpha(queryAligned[i])) {
+ baseCount++;
+ if (!foundFirstBase) { foundFirstBase = true; firstBaseSpot = i; }
+ }
+
+ //eliminate opening .'s
+ if (foundFirstBase) { leftQuery += queryAligned[i]; }
+ //if you have 1/3
+ if (baseCount >= numBases) { leftSpot = i; break; } //first 1/3
+ }
+
+ //right side - count through another 1/3, so you are at last third
+ baseCount = 0;
+ int rightSpot = 0;
+ for (int i = leftSpot; i < queryAligned.length(); i++) {
+ //if you are a base
+ if (isalpha(queryAligned[i])) { baseCount++; }
+ //if you have 1/3
+ if (baseCount >= numBases) { rightSpot = i; break; } //last 1/3
+ }
+
+ //trim end
+ //find last position in query that is a non gap character
+ int lastBaseSpot = queryAligned.length()-1;
+ for (int j = queryAligned.length()-1; j >= 0; j--) {
+ if (isalpha(queryAligned[j])) {
+ lastBaseSpot = j;
+ break;
+ }
+ }
+ rightQuery = queryAligned.substr(rightSpot, (lastBaseSpot-rightSpot)); //sequence from pos spot to end
+
+ Sequence queryLeft(querySeq->getName(), leftQuery);
+ Sequence queryRight(querySeq->getName(), rightQuery);
+//cout << querySeq->getName() << '\t' << leftSpot << '\t' << rightSpot << '\t' << firstBaseSpot << '\t' << lastBaseSpot << endl;
+//cout << queryUnAligned.length() << '\t' << queryLeft.getUnaligned().length() << '\t' << queryRight.getUnaligned().length() << endl;
+ for(int j = 0; j < db.size(); j++){
+
+ string dbAligned = db[j]->getAligned();
+ string leftDB = dbAligned.substr(firstBaseSpot, (leftSpot-firstBaseSpot+1)); //first 1/3 of the sequence
+ string rightDB = dbAligned.substr(rightSpot, (lastBaseSpot-rightSpot)); //last 1/3 of the sequence
+
+ Sequence dbLeft(db[j]->getName(), leftDB);
+ Sequence dbRight(db[j]->getName(), rightDB);
+
+ distcalculator->calcDist(queryLeft, dbLeft);
+ float distLeft = distcalculator->getDist();
+
+ distcalculator->calcDist(queryRight, dbRight);
+ float distRight = distcalculator->getDist();
+
+ SeqDist subjectLeft;
+ subjectLeft.seq = db[j];
+ subjectLeft.dist = distLeft;
+ subjectLeft.index = j;
+
+ distsLeft.push_back(subjectLeft);
+
+ SeqDist subjectRight;
+ subjectRight.seq = db[j];
+ subjectRight.dist = distRight;
+ subjectRight.index = j;
+
+ distsRight.push_back(subjectRight);
+
+ }
+
+ delete distcalculator;
+
+ //sort by smallest distance
+ sort(distsRight.begin(), distsRight.end(), compareSeqDist);
+ sort(distsLeft.begin(), distsLeft.end(), compareSeqDist);
+
+ //merge results
+ map<string, string> seen;
+ map<string, string>::iterator it;
+
+ vector<SeqDist> dists;
+ float lastRight = distsRight[0].dist;
+ float lastLeft = distsLeft[0].dist;
+ int lasti = 0;
+ for (int i = 0; i < distsLeft.size(); i++) {
+ //add left if you havent already
+ it = seen.find(distsLeft[i].seq->getName());
+ if (it == seen.end()) {
+ dists.push_back(distsLeft[i]);
+ seen[distsLeft[i].seq->getName()] = distsLeft[i].seq->getName();
+ lastLeft = distsLeft[i].dist;
+ }
+
+ //add right if you havent already
+ it = seen.find(distsRight[i].seq->getName());
+ if (it == seen.end()) {
+ dists.push_back(distsRight[i]);
+ seen[distsRight[i].seq->getName()] = distsRight[i].seq->getName();
+ lastRight = distsRight[i].dist;
+ }
+
+ if (dists.size() > numWanted) { lasti = i; break; } //you have enough results
+ }
+
+ //add in dups
+ lasti++;
+ int i = lasti;
+ while (i < distsLeft.size()) {
+ if (distsLeft[i].dist == lastLeft) { dists.push_back(distsLeft[i]); numWanted++; }
+ else { break; }
+ i++;
+ }
+
+ i = lasti;
+ while (i < distsRight.size()) {
+ if (distsRight[i].dist == lastRight) { dists.push_back(distsRight[i]); numWanted++; }
+ else { break; }
+ i++;
+ }
+
+//cout << numWanted << endl;
+ for (int i = 0; i < numWanted; i++) {
+//cout << dists[i].seq->getName() << '\t' << dists[i].dist << endl;
+ Sequence* temp = new Sequence(dists[i].seq->getName(), dists[i].seq->getAligned()); //have to make a copy so you can trim and filter without stepping on eachother.
+ seqsMatches.push_back(temp);
+ indexes.push_back(dists[i].index);
+ }
+
+ return seqsMatches;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "DeCalculator", "findClosest");
+ exit(1);
+ }
+}
+//***************************************************************************************************************
+Sequence* DeCalculator::findClosest(Sequence* querySeq, vector<Sequence*> db) {
+ try {
+
+ Sequence* seqsMatch;
+
+ Dist* distcalculator = new eachGapDist();
+ int index = 0;
+ int smallest = 1000000;
+
+ for(int j = 0; j < db.size(); j++){
+
+ distcalculator->calcDist(*querySeq, *db[j]);
+ float dist = distcalculator->getDist();
+
+ if (dist < smallest) {
+ smallest = dist;
+ index = j;
+ }
+ }
+
+ delete distcalculator;
+
+ seqsMatch = new Sequence(db[index]->getName(), db[index]->getAligned()); //have to make a copy so you can trim and filter without stepping on eachother.
+
+ return seqsMatch;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "DeCalculator", "findClosest");
+ exit(1);
+ }
+}
+/***************************************************************************************************************/
+map<int, int> DeCalculator::trimSeqs(Sequence* query, vector<Sequence*> topMatches) {
+ try {
+
+ int frontPos = 0; //should contain first position in all seqs that is not a gap character
+ int rearPos = query->getAligned().length();
+
+ //********find first position in topMatches that is a non gap character***********//
+ //find first position all query seqs that is a non gap character
+ for (int i = 0; i < topMatches.size(); i++) {
+
+ string aligned = topMatches[i]->getAligned();
+ int pos = 0;
+
+ //find first spot in this seq
+ for (int j = 0; j < aligned.length(); j++) {
+ if (isalpha(aligned[j])) {
+ pos = j;
+ break;
+ }
+ }
+
+ //save this spot if it is the farthest
+ if (pos > frontPos) { frontPos = pos; }
+ }
+
+
+ string aligned = query->getAligned();
+ int pos = 0;
+
+ //find first position in query that is a non gap character
+ for (int j = 0; j < aligned.length(); j++) {
+ if (isalpha(aligned[j])) {
+ pos = j;
+ break;
+ }
+ }
+
+ //save this spot if it is the farthest
+ if (pos > frontPos) { frontPos = pos; }
+
+
+ //********find last position in topMatches that is a non gap character***********//
+ for (int i = 0; i < topMatches.size(); i++) {
+
+ string aligned = topMatches[i]->getAligned();
+ int pos = aligned.length();
+
+ //find first spot in this seq
+ for (int j = aligned.length()-1; j >= 0; j--) {
+ if (isalpha(aligned[j])) {
+ pos = j;
+ break;
+ }
+ }
+
+ //save this spot if it is the farthest
+ if (pos < rearPos) { rearPos = pos; }
+ }
+
+
+ aligned = query->getAligned();
+ pos = aligned.length();
+
+ //find last position in query that is a non gap character
+ for (int j = aligned.length()-1; j >= 0; j--) {
+ if (isalpha(aligned[j])) {
+ pos = j;
+ break;
+ }
+ }
+
+ //save this spot if it is the farthest
+ if (pos < rearPos) { rearPos = pos; }
+
+ //check to make sure that is not whole seq
+ if ((rearPos - frontPos - 1) <= 0) { m->mothurOut("Error, when I trim your sequences, the entire sequence is trimmed."); m->mothurOutEndLine(); exit(1); }
+//cout << query->getName() << " front = " << frontPos << " rear = " << rearPos << endl;
+ //trim query
+ string newAligned = query->getAligned();
+ newAligned = newAligned.substr(frontPos, (rearPos-frontPos+1));
+ query->setAligned(newAligned);
+
+ //trim topMatches
+ for (int i = 0; i < topMatches.size(); i++) {
+ newAligned = topMatches[i]->getAligned();
+ newAligned = newAligned.substr(frontPos, (rearPos-frontPos+1));
+ topMatches[i]->setAligned(newAligned);
+ }
+
+ map<int, int> trimmedPos;
+
+ for (int i = 0; i < newAligned.length(); i++) {
+ trimmedPos[i] = i+frontPos;
+ }
+
+ return trimmedPos;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "DeCalculator", "trimSequences");
+ exit(1);
+ }
+
+}
+//***************************************************************************************************************
+
projects / mothur.git / blobdiff