--- /dev/null
+/*
+ * clearcutcommand.cpp
+ * Mothur
+ *
+ * Created by westcott on 5/11/10.
+ * Copyright 2010 Schloss Lab. All rights reserved.
+ *
+ */
+
+#include "clearcutcommand.h"
+#ifdef __cplusplus
+extern "C" {
+#endif
+#include "clearcut.h"
+#ifdef __cplusplus
+}
+#endif
+
+//**********************************************************************************************************************
+vector<string> ClearcutCommand::setParameters(){
+ try {
+ CommandParameter pphylip("phylip", "InputTypes", "", "", "FastaPhylip", "FastaPhylip", "none",false,false); parameters.push_back(pphylip);
+ CommandParameter pfasta("fasta", "InputTypes", "", "", "FastaPhylip", "FastaPhylip", "none",false,false); parameters.push_back(pfasta);
+ CommandParameter pverbose("verbose", "Boolean", "", "F", "", "", "",false,false); parameters.push_back(pverbose);
+ CommandParameter pquiet("quiet", "Boolean", "", "F", "", "", "",false,false); parameters.push_back(pquiet);
+ CommandParameter pversion("version", "Boolean", "", "F", "", "", "",false,false); parameters.push_back(pversion);
+ CommandParameter pseed("seed", "String", "", "", "*", "", "",false,false); parameters.push_back(pseed);
+ CommandParameter pnorandom("norandom", "Boolean", "", "F", "", "", "",false,false); parameters.push_back(pnorandom);
+ CommandParameter pshuffle("shuffle", "Boolean", "", "F", "", "", "",false,false); parameters.push_back(pshuffle);
+ CommandParameter pneighbor("neighbor", "Boolean", "", "T", "", "", "",false,false); parameters.push_back(pneighbor);
+ CommandParameter pexpblen("expblen", "Boolean", "", "F", "", "", "",false,false); parameters.push_back(pexpblen);
+ CommandParameter pexpdist("expdist", "Boolean", "", "F", "", "", "",false,false); parameters.push_back(pexpdist);
+ CommandParameter pDNA("DNA", "Boolean", "", "F", "", "", "",false,false); parameters.push_back(pDNA);
+ CommandParameter pprotein("protein", "Boolean", "", "F", "", "", "",false,false); parameters.push_back(pprotein);
+ CommandParameter pjukes("jukes", "Boolean", "", "F", "", "", "",false,false); parameters.push_back(pjukes);
+ CommandParameter pkimura("kimura", "Boolean", "", "F", "", "", "",false,false); parameters.push_back(pkimura);
+ CommandParameter pstdout("stdout", "Boolean", "", "F", "", "", "",false,false); parameters.push_back(pstdout);
+ CommandParameter pntrees("ntrees", "Number", "", "1", "", "", "",false,false); parameters.push_back(pntrees);
+ CommandParameter pmatrixout("matrixout", "String", "", "", "", "", "",false,false); parameters.push_back(pmatrixout);
+ CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
+ CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir);
+
+ vector<string> myArray;
+ for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); }
+ return myArray;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClearcutCommand", "setParameters");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+string ClearcutCommand::getHelpString(){
+ try {
+ string helpString = "";
+ helpString += "The clearcut command interfaces mothur with the clearcut program written by Initiative for Bioinformatics and Evolutionary Studies (IBEST) at the University of Idaho.\n";
+ helpString += "For more information about clearcut refer to http://bioinformatics.hungry.com/clearcut/ \n";
+ helpString += "The clearcut command parameters are phylip, fasta, version, verbose, quiet, seed, norandom, shuffle, neighbor, expblen, expdist, ntrees, matrixout, stdout, kimura, jukes, protein, DNA. \n";
+ helpString += "The phylip parameter allows you to enter your phylip formatted distance matrix. \n";
+ helpString += "The fasta parameter allows you to enter your aligned fasta file, if you enter a fastafile you specify if the sequences are DNA or protein using the DNA or protein parameters. \n";
+
+ helpString += "The version parameter prints out the version of clearcut you are using, default=F. \n";
+ helpString += "The verbose parameter prints out more output from clearcut, default=F. \n";
+ helpString += "The quiet parameter turns on silent operation mode, default=F. \n";
+ helpString += "The seed parameter allows you to explicitly set the PRNG seed to a specific value. \n";
+ helpString += "The norandom parameter allows you to attempt joins deterministically, default=F. \n";
+ helpString += "The shuffle parameter allows you to randomly shuffle the distance matrix, default=F. \n";
+ helpString += "The neighbor parameter allows you to use traditional Neighbor-Joining algorithm, default=T. \n";
+
+ helpString += "The DNA parameter allows you to indicate your fasta file contains DNA sequences, default=F. \n";
+ helpString += "The protein parameter allows you to indicate your fasta file contains protein sequences, default=F. \n";
+
+ helpString += "The stdout parameter outputs your tree to STDOUT, default=F. \n";
+ helpString += "The matrixout parameter allows you to specify a filename to output a distance matrix to. \n";
+ helpString += "The ntrees parameter allows you to specify the number of output trees, default=1. \n";
+ helpString += "The expblen parameter allows you to use exponential notation for branch lengths, default=F. \n";
+ helpString += "The expdist parameter allows you to use exponential notation for distance outputs, default=F. \n";
+
+ helpString += "The clearcut command should be in the following format: \n";
+ helpString += "clearcut(phylip=yourDistanceFile) \n";
+ helpString += "Example: clearcut(phylip=abrecovery.phylip.dist) \n";
+ return helpString;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClearcutCommand", "getHelpString");
+ exit(1);
+ }
+}
+
+//**********************************************************************************************************************
+ClearcutCommand::ClearcutCommand(){
+ try {
+ abort = true; calledHelp = true;
+ setParameters();
+ vector<string> tempOutNames;
+ outputTypes["tree"] = tempOutNames;
+ outputTypes["matrixout"] = tempOutNames;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClearcutCommand", "ClearcutCommand");
+ exit(1);
+ }
+}
+/**************************************************************************************/
+ClearcutCommand::ClearcutCommand(string option) {
+ try {
+ abort = false; calledHelp = false;
+
+ //allow user to run help
+ if(option == "help") { help(); abort = true; calledHelp = true; }
+ else if(option == "citation") { citation(); abort = true; calledHelp = true;}
+
+ else {
+ vector<string> myArray = setParameters();
+
+ OptionParser parser(option);
+ map<string,string> parameters = parser.getParameters();
+
+ ValidParameters validParameter;
+ map<string, string>::iterator it;
+
+ //check to make sure all parameters are valid for command
+ for (it = parameters.begin(); it != parameters.end(); it++) {
+ if (validParameter.isValidParameter(it->first, myArray, it->second) != true) { abort = true; }
+ }
+
+ //initialize outputTypes
+ vector<string> tempOutNames;
+ outputTypes["tree"] = tempOutNames;
+ outputTypes["matrixout"] = tempOutNames;
+
+ //if the user changes the input directory command factory will send this info to us in the output parameter
+ string inputDir = validParameter.validFile(parameters, "inputdir", false);
+ if (inputDir == "not found"){ inputDir = ""; }
+ else {
+ string path;
+ it = parameters.find("fasta");
+ //user has given a template file
+ if(it != parameters.end()){
+ path = m->hasPath(it->second);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { parameters["fasta"] = inputDir + it->second; }
+ }
+
+ it = parameters.find("phylip");
+ //user has given a template file
+ if(it != parameters.end()){
+ path = m->hasPath(it->second);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { parameters["phylip"] = inputDir + it->second; }
+ }
+ }
+
+ //check for required parameters
+ fastafile = validParameter.validFile(parameters, "fasta", true);
+ if (fastafile == "not open") { fastafile = ""; abort = true; }
+ else if (fastafile == "not found") { fastafile = ""; }
+ else { inputFile = fastafile; m->setFastaFile(fastafile); }
+
+ phylipfile = validParameter.validFile(parameters, "phylip", true);
+ if (phylipfile == "not open") { phylipfile = ""; abort = true; }
+ else if (phylipfile == "not found") { phylipfile = ""; }
+ else { inputFile = phylipfile; m->setPhylipFile(phylipfile); }
+
+ if ((phylipfile == "") && (fastafile == "")) {
+ //is there are current file available for either of these?
+ //give priority to phylip, then fasta
+ phylipfile = m->getPhylipFile();
+ if (phylipfile != "") { inputFile = phylipfile; m->mothurOut("Using " + phylipfile + " as input file for the phylip parameter."); m->mothurOutEndLine(); }
+ else {
+ fastafile = m->getFastaFile();
+ if (fastafile != "") { inputFile = fastafile; m->mothurOut("Using " + fastafile + " as input file for the fasta parameter."); m->mothurOutEndLine(); }
+ else {
+ m->mothurOut("No valid current files. You must provide a phylip or fasta file before you can use the clearcut command."); m->mothurOutEndLine();
+ abort = true;
+ }
+ }
+ }
+ if ((phylipfile != "") && (fastafile != "")) { m->mothurOut("You must provide either a phylip formatted distance matrix or an aligned fasta file, not BOTH."); m->mothurOutEndLine(); abort=true; }
+
+
+ //if the user changes the output directory command factory will send this info to us in the output parameter
+ outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){ outputDir = m->hasPath(inputFile); }
+
+ string temp;
+ temp = validParameter.validFile(parameters, "version", false); if (temp == "not found"){ temp = "F"; }
+ version = m->isTrue(temp);
+
+ temp = validParameter.validFile(parameters, "verbose", false); if (temp == "not found"){ temp = "F"; }
+ verbose = m->isTrue(temp);
+
+ temp = validParameter.validFile(parameters, "quiet", false); if (temp == "not found"){ temp = "F"; }
+ quiet = m->isTrue(temp);
+
+ seed = validParameter.validFile(parameters, "seed", false); if (seed == "not found"){ seed = "*"; }
+
+ temp = validParameter.validFile(parameters, "norandom", false); if (temp == "not found"){ temp = "F"; }
+ norandom = m->isTrue(temp);
+
+ temp = validParameter.validFile(parameters, "shuffle", false); if (temp == "not found"){ temp = "F"; }
+ shuffle = m->isTrue(temp);
+
+ temp = validParameter.validFile(parameters, "neighbor", false); if (temp == "not found"){ temp = "T"; }
+ neighbor = m->isTrue(temp);
+
+ temp = validParameter.validFile(parameters, "DNA", false); if (temp == "not found"){ temp = "F"; }
+ DNA = m->isTrue(temp);
+
+ temp = validParameter.validFile(parameters, "protein", false); if (temp == "not found"){ temp = "F"; }
+ protein = m->isTrue(temp);
+
+ temp = validParameter.validFile(parameters, "jukes", false); if (temp == "not found"){ temp = "F"; }
+ jukes = m->isTrue(temp);
+
+ temp = validParameter.validFile(parameters, "kimura", false); if (temp == "not found"){ temp = "F"; }
+ kimura = m->isTrue(temp);
+
+ temp = validParameter.validFile(parameters, "stdout", false); if (temp == "not found"){ temp = "F"; }
+ stdoutWanted = m->isTrue(temp);
+
+ matrixout = validParameter.validFile(parameters, "matrixout", false); if (matrixout == "not found"){ matrixout = ""; }
+
+ ntrees = validParameter.validFile(parameters, "ntrees", false); if (ntrees == "not found"){ ntrees = "1"; }
+
+ temp = validParameter.validFile(parameters, "expblen", false); if (temp == "not found"){ temp = "F"; }
+ expblen = m->isTrue(temp);
+
+ temp = validParameter.validFile(parameters, "expdist", false); if (temp == "not found"){ temp = "F"; }
+ expdist = m->isTrue(temp);
+
+ if ((fastafile != "") && ((!DNA) && (!protein))) { m->mothurOut("You must specify the type of sequences you are using: DNA or protein"); m->mothurOutEndLine(); abort=true; }
+ }
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClearcutCommand", "ClearcutCommand");
+ exit(1);
+ }
+}
+/**************************************************************************************/
+int ClearcutCommand::execute() {
+ try {
+
+ if (abort == true) { if (calledHelp) { return 0; } return 2; }
+
+ //prepare filename
+ string outputName = outputDir + m->getRootName(m->getSimpleName(inputFile)) + "tre";
+ outputNames.push_back(outputName); outputTypes["tree"].push_back(outputName);
+
+ vector<char*> cPara;
+
+ char* tempClearcut = new char[8];
+ strcpy(tempClearcut, "clearcut");
+ cPara.push_back(tempClearcut);
+
+ //you gave us a distance matrix
+ if (phylipfile != "") { char* temp = new char[10]; strcpy(temp, "--distance"); cPara.push_back(temp); }
+
+ //you gave us a fastafile
+ if (fastafile != "") { char* temp = new char[11]; strcpy(temp, "--alignment"); cPara.push_back(temp); }
+
+ if (version) { char* temp = new char[9]; strcpy(temp, "--version"); cPara.push_back(temp); }
+ if (verbose) { char* temp = new char[9]; strcpy(temp, "--verbose"); cPara.push_back(temp); }
+ if (quiet) { char* temp = new char[7]; strcpy(temp, "--quiet"); cPara.push_back(temp); }
+ if (seed != "*") {
+ string tempSeed = "--seed=" + seed;
+ char* temp = new char[tempSeed.length()];
+ strcpy(temp, tempSeed.c_str());
+ cPara.push_back(temp);
+ }
+ if (norandom) { char* temp = new char[10]; strcpy(temp, "--norandom"); cPara.push_back(temp); }
+ if (shuffle) { char* temp = new char[9]; strcpy(temp, "--shuffle"); cPara.push_back(temp); }
+ if (neighbor) { char* temp = new char[10]; strcpy(temp, "--neighbor"); cPara.push_back(temp); }
+
+ string tempIn = "--in=" + inputFile;
+ char* tempI = new char[tempIn.length()];
+ strcpy(tempI, tempIn.c_str());
+ cPara.push_back(tempI);
+
+ if (stdoutWanted) { char* temp = new char[8]; strcpy(temp, "--stdout"); cPara.push_back(temp); }
+ else{
+ string tempOut = "--out=" + outputName;
+
+ char* temp = new char[tempOut.length()];
+ strcpy(temp, tempOut.c_str());
+ cPara.push_back(temp);
+ }
+
+ if (DNA) { char* temp = new char[5]; strcpy(temp, "--DNA"); cPara.push_back(temp); }
+ if (protein) { char* temp = new char[9]; strcpy(temp, "--protein"); cPara.push_back(temp); }
+ if (jukes) { char* temp = new char[7]; strcpy(temp, "--jukes"); cPara.push_back(temp); }
+ if (kimura) { char* temp = new char[8]; strcpy(temp, "--kimura"); cPara.push_back(temp); }
+ if (matrixout != "") {
+ string tempMatrix = "--matrixout=" + outputDir + matrixout;
+ char* temp = new char[tempMatrix.length()];
+ strcpy(temp, tempMatrix.c_str());
+ cPara.push_back(temp);
+ outputNames.push_back((outputDir + matrixout));
+ outputTypes["matrixout"].push_back((outputDir + matrixout));
+ }
+
+ if (ntrees != "1") {
+ string tempNtrees = "--ntrees=" + ntrees;
+ char* temp = new char[tempNtrees.length()];
+ strcpy(temp, tempNtrees.c_str());
+ cPara.push_back(temp);
+ }
+
+ if (expblen) { char* temp = new char[9]; strcpy(temp, "--expblen"); cPara.push_back(temp); }
+ if (expdist) { char* temp = new char[9]; strcpy(temp, "--expdist"); cPara.push_back(temp); }
+
+ char** clearcutParameters;
+ clearcutParameters = new char*[cPara.size()];
+ for (int i = 0; i < cPara.size(); i++) { clearcutParameters[i] = cPara[i]; }
+ int numArgs = cPara.size();
+
+ clearcut_main(numArgs, clearcutParameters);
+
+ //free memory
+ for(int i = 0; i < cPara.size(); i++) { delete[] cPara[i]; }
+ delete[] clearcutParameters;
+
+ if (!stdoutWanted) {
+
+ //set first tree file as new current treefile
+ string currentTree = "";
+ itTypes = outputTypes.find("tree");
+ if (itTypes != outputTypes.end()) {
+ if ((itTypes->second).size() != 0) { currentTree = (itTypes->second)[0]; m->setTreeFile(currentTree); }
+ }
+
+ m->mothurOutEndLine();
+ m->mothurOut("Output File Names: "); m->mothurOutEndLine();
+ for (int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i]); m->mothurOutEndLine(); }
+ m->mothurOutEndLine();
+ }
+
+ return 0;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClearcutCommand", "execute");
+ exit(1);
+ }
+}
+/**************************************************************************************/
+
+
+
+