--- /dev/null
+/*
+ * classifyotucommand.cpp
+ * Mothur
+ *
+ * Created by westcott on 6/1/10.
+ * Copyright 2010 Schloss Lab. All rights reserved.
+ *
+ */
+
+#include "classifyotucommand.h"
+#include "phylotree.h"
+#include "phylosummary.h"
+
+//**********************************************************************************************************************
+vector<string> ClassifyOtuCommand::setParameters(){
+ try {
+ CommandParameter plist("list", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(plist);
+ CommandParameter ptaxonomy("taxonomy", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(ptaxonomy);
+ CommandParameter preftaxonomy("reftaxonomy", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(preftaxonomy);
+ CommandParameter pname("name", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(pname);
+ CommandParameter pgroup("group", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(pgroup);
+ CommandParameter plabel("label", "String", "", "", "", "", "",false,false); parameters.push_back(plabel);
+ CommandParameter pbasis("basis", "Multiple", "otu-sequence", "otu", "", "", "",false,false); parameters.push_back(pbasis);
+ CommandParameter pcutoff("cutoff", "Number", "", "51", "", "", "",false,true); parameters.push_back(pcutoff);
+ CommandParameter pprobs("probs", "Boolean", "", "T", "", "", "",false,false); parameters.push_back(pprobs);
+ CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
+ CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir);
+
+ vector<string> myArray;
+ for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); }
+ return myArray;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClassifyOtuCommand", "setParameters");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+string ClassifyOtuCommand::getHelpString(){
+ try {
+ string helpString = "";
+ helpString += "The classify.otu command parameters are list, taxonomy, reftaxonomy, name, group, cutoff, label, basis and probs. The taxonomy and list parameters are required unless you have a valid current file.\n";
+ helpString += "The reftaxonomy parameter allows you give the name of the reference taxonomy file used when you classified your sequences. Providing it will keep the rankIDs in the summary file static.\n";
+ helpString += "The name parameter allows you add a names file with your taxonomy file.\n";
+ helpString += "The group parameter allows you provide a group file to use in creating the summary file breakdown.\n";
+ helpString += "The basis parameter allows you indicate what you want the summary file to represent, options are otu and sequence. Default is otu.\n";
+ helpString += "For example consider the following basis=sequence could give Clostridiales 3 105 16 43 46, where 105 is the total number of sequences whose otu classified to Clostridiales.\n";
+ helpString += "16 is the number of sequences in the otus from groupA, 43 is the number of sequences in the otus from groupB, and 46 is the number of sequences in the otus from groupC.\n";
+ helpString += "Now for basis=otu could give Clostridiales 3 7 6 1 2, where 7 is the number of otus that classified to Clostridiales.\n";
+ helpString += "6 is the number of otus containing sequences from groupA, 1 is the number of otus containing sequences from groupB, and 2 is the number of otus containing sequences from groupC.\n";
+ helpString += "The label parameter allows you to select what distance levels you would like a output files created for, and is separated by dashes.\n";
+ helpString += "The default value for label is all labels in your inputfile.\n";
+ helpString += "The cutoff parameter allows you to specify a consensus confidence threshold for your taxonomy. The default is 51, meaning 51%. Cutoff cannot be below 51.\n";
+ helpString += "The probs parameter shuts off the outputting of the consensus confidence results. The default is true, meaning you want the confidence to be shown.\n";
+ helpString += "The classify.otu command should be in the following format: classify.otu(taxonomy=yourTaxonomyFile, list=yourListFile, name=yourNamesFile, label=yourLabels).\n";
+ helpString += "Example classify.otu(taxonomy=abrecovery.silva.full.taxonomy, list=abrecovery.fn.list, label=0.10).\n";
+ helpString += "Note: No spaces between parameter labels (i.e. list), '=' and parameters (i.e.yourListFile).\n";
+ return helpString;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClassifyOtuCommand", "getHelpString");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+ClassifyOtuCommand::ClassifyOtuCommand(){
+ try {
+ abort = true; calledHelp = true;
+ setParameters();
+ vector<string> tempOutNames;
+ outputTypes["constaxonomy"] = tempOutNames;
+ outputTypes["taxsummary"] = tempOutNames;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClassifyOtuCommand", "ClassifyOtuCommand");
+ exit(1);
+ }
+}
+
+//**********************************************************************************************************************
+ClassifyOtuCommand::ClassifyOtuCommand(string option) {
+ try{
+ abort = false; calledHelp = false;
+ allLines = 1;
+ labels.clear();
+
+ //allow user to run help
+ if (option == "help") {
+ help(); abort = true; calledHelp = true;
+ }else if(option == "citation") { citation(); abort = true; calledHelp = true;}
+ else {
+ vector<string> myArray = setParameters();
+
+ OptionParser parser(option);
+ map<string, string> parameters = parser.getParameters();
+
+ ValidParameters validParameter;
+ map<string, string>::iterator it;
+
+ //check to make sure all parameters are valid for command
+ for (it = parameters.begin(); it != parameters.end(); it++) {
+ if (validParameter.isValidParameter(it->first, myArray, it->second) != true) { abort = true; }
+ }
+
+ //initialize outputTypes
+ vector<string> tempOutNames;
+ outputTypes["constaxonomy"] = tempOutNames;
+ outputTypes["taxsummary"] = tempOutNames;
+
+ //if the user changes the input directory command factory will send this info to us in the output parameter
+ string inputDir = validParameter.validFile(parameters, "inputdir", false);
+ if (inputDir == "not found"){ inputDir = ""; }
+ else {
+ string path;
+ it = parameters.find("list");
+ //user has given a template file
+ if(it != parameters.end()){
+ path = m->hasPath(it->second);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { parameters["list"] = inputDir + it->second; }
+ }
+
+ it = parameters.find("name");
+ //user has given a template file
+ if(it != parameters.end()){
+ path = m->hasPath(it->second);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { parameters["name"] = inputDir + it->second; }
+ }
+
+ it = parameters.find("taxonomy");
+ //user has given a template file
+ if(it != parameters.end()){
+ path = m->hasPath(it->second);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { parameters["taxonomy"] = inputDir + it->second; }
+ }
+
+ it = parameters.find("reftaxonomy");
+ //user has given a template file
+ if(it != parameters.end()){
+ path = m->hasPath(it->second);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { parameters["reftaxonomy"] = inputDir + it->second; }
+ }
+
+ it = parameters.find("group");
+ //user has given a template file
+ if(it != parameters.end()){
+ path = m->hasPath(it->second);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { parameters["group"] = inputDir + it->second; }
+ }
+ }
+
+
+ //if the user changes the output directory command factory will send this info to us in the output parameter
+ outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){ outputDir = ""; }
+
+ //check for required parameters
+ listfile = validParameter.validFile(parameters, "list", true);
+ if (listfile == "not found") {
+ //if there is a current list file, use it
+ listfile = m->getListFile();
+ if (listfile != "") { m->mothurOut("Using " + listfile + " as input file for the list parameter."); m->mothurOutEndLine(); }
+ else { m->mothurOut("You have no current listfile and the list parameter is required."); m->mothurOutEndLine(); abort = true; }
+ }
+ else if (listfile == "not open") { abort = true; }
+ else { m->setListFile(listfile); }
+
+ taxfile = validParameter.validFile(parameters, "taxonomy", true);
+ if (taxfile == "not found") { //if there is a current list file, use it
+ taxfile = m->getTaxonomyFile();
+ if (taxfile != "") { m->mothurOut("Using " + taxfile + " as input file for the taxonomy parameter."); m->mothurOutEndLine(); }
+ else { m->mothurOut("You have no current taxonomy file and the taxonomy parameter is required."); m->mothurOutEndLine(); abort = true; }
+ }
+ else if (taxfile == "not open") { abort = true; }
+ else { m->setTaxonomyFile(taxfile); }
+
+ refTaxonomy = validParameter.validFile(parameters, "reftaxonomy", true);
+ if (refTaxonomy == "not found") { refTaxonomy = ""; m->mothurOut("reftaxonomy is not required, but if given will keep the rankIDs in the summary file static."); m->mothurOutEndLine(); }
+ else if (refTaxonomy == "not open") { abort = true; }
+
+ namefile = validParameter.validFile(parameters, "name", true);
+ if (namefile == "not open") { namefile = ""; abort = true; }
+ else if (namefile == "not found") { namefile = ""; }
+ else { m->setNameFile(namefile); }
+
+ groupfile = validParameter.validFile(parameters, "group", true);
+ if (groupfile == "not open") { abort = true; }
+ else if (groupfile == "not found") { groupfile = ""; }
+ else { m->setGroupFile(groupfile); }
+
+ //check for optional parameter and set defaults
+ // ...at some point should added some additional type checking...
+ label = validParameter.validFile(parameters, "label", false);
+ if (label == "not found") { label = ""; allLines = 1; }
+ else {
+ if(label != "all") { m->splitAtDash(label, labels); allLines = 0; }
+ else { allLines = 1; }
+ }
+
+ basis = validParameter.validFile(parameters, "basis", false);
+ if (basis == "not found") { basis = "otu"; }
+
+ if ((basis != "otu") && (basis != "sequence")) { m->mothurOut("Invalid option for basis. basis options are otu and sequence, using otu."); m->mothurOutEndLine(); }
+
+ string temp = validParameter.validFile(parameters, "cutoff", false); if (temp == "not found") { temp = "51"; }
+ m->mothurConvert(temp, cutoff);
+
+ temp = validParameter.validFile(parameters, "probs", false); if (temp == "not found"){ temp = "true"; }
+ probs = m->isTrue(temp);
+
+
+ if ((cutoff < 51) || (cutoff > 100)) { m->mothurOut("cutoff must be above 50, and no greater than 100."); m->mothurOutEndLine(); abort = true; }
+
+ if (namefile == ""){
+ vector<string> files; files.push_back(taxfile);
+ parser.getNameFile(files);
+ }
+
+ }
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClassifyOtuCommand", "ClassifyOtuCommand");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+
+int ClassifyOtuCommand::execute(){
+ try {
+
+ if (abort == true) { if (calledHelp) { return 0; } return 2; }
+
+ //if user gave a namesfile then use it
+ if (namefile != "") { readNamesFile(); }
+
+ //read taxonomy file and save in map for easy access in building bin trees
+ readTaxonomyFile();
+
+ if (m->control_pressed) { return 0; }
+
+ input = new InputData(listfile, "list");
+ list = input->getListVector();
+ string lastLabel = list->getLabel();
+
+ //if the users enters label "0.06" and there is no "0.06" in their file use the next lowest label.
+ set<string> processedLabels;
+ set<string> userLabels = labels;
+
+ if (m->control_pressed) { outputTypes.clear(); delete input; delete list; for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; }
+
+ while((list != NULL) && ((allLines == 1) || (userLabels.size() != 0))) {
+
+ if (allLines == 1 || labels.count(list->getLabel()) == 1){
+
+ m->mothurOut(list->getLabel() + "\t" + toString(list->size())); m->mothurOutEndLine();
+ process(list);
+ if (m->control_pressed) { outputTypes.clear(); for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } delete input; delete list; return 0; }
+
+ processedLabels.insert(list->getLabel());
+ userLabels.erase(list->getLabel());
+ }
+
+ if ((m->anyLabelsToProcess(list->getLabel(), userLabels, "") == true) && (processedLabels.count(lastLabel) != 1)) {
+ string saveLabel = list->getLabel();
+
+ delete list;
+ list = input->getListVector(lastLabel);
+ m->mothurOut(list->getLabel() + "\t" + toString(list->size())); m->mothurOutEndLine();
+ process(list);
+
+
+ if (m->control_pressed) { outputTypes.clear(); for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } delete input; delete list; return 0; }
+
+ processedLabels.insert(list->getLabel());
+ userLabels.erase(list->getLabel());
+
+ //restore real lastlabel to save below
+ list->setLabel(saveLabel);
+ }
+
+ lastLabel = list->getLabel();
+
+ delete list;
+ list = input->getListVector();
+ }
+
+ //output error messages about any remaining user labels
+ bool needToRun = false;
+ for (set<string>::iterator it = userLabels.begin(); it != userLabels.end(); it++) {
+ m->mothurOut("Your file does not include the label " + (*it));
+ if (processedLabels.count(lastLabel) != 1) {
+ m->mothurOut(". I will use " + lastLabel + "."); m->mothurOutEndLine();
+ needToRun = true;
+ }else {
+ m->mothurOut(". Please refer to " + lastLabel + "."); m->mothurOutEndLine();
+ }
+ }
+
+ //run last label if you need to
+ if (needToRun == true) {
+ if (list != NULL) { delete list; }
+ list = input->getListVector(lastLabel);
+ m->mothurOut(list->getLabel() + "\t" + toString(list->size())); m->mothurOutEndLine();
+
+ process(list);
+ delete list;
+
+ if (m->control_pressed) { outputTypes.clear(); for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } delete input; delete list; return 0; }
+ }
+
+ delete input;
+
+ if (m->control_pressed) { outputTypes.clear(); for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; }
+
+ m->mothurOutEndLine();
+ m->mothurOut("Output File Names: "); m->mothurOutEndLine();
+ for (int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i]); m->mothurOutEndLine(); }
+ m->mothurOutEndLine();
+
+ return 0;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClassifyOtuCommand", "execute");
+ exit(1);
+ }
+}
+
+//**********************************************************************************************************************
+int ClassifyOtuCommand::readNamesFile() {
+ try {
+
+ ifstream inNames;
+ m->openInputFile(namefile, inNames);
+
+ string name, names;
+
+ while(!inNames.eof()){
+ inNames >> name; //read from first column A
+ inNames >> names; //read from second column A,B,C,D
+ m->gobble(inNames);
+
+ //parse names into vector
+ vector<string> theseNames;
+ m->splitAtComma(names, theseNames);
+
+ for (int i = 0; i < theseNames.size(); i++) { nameMap[theseNames[i]] = name; }
+
+ if (m->control_pressed) { inNames.close(); nameMap.clear(); return 0; }
+ }
+ inNames.close();
+
+ return 0;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClassifyOtuCommand", "readNamesFile");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+int ClassifyOtuCommand::readTaxonomyFile() {
+ try {
+
+ ifstream in;
+ m->openInputFile(taxfile, in);
+
+ string name, tax;
+
+ while(!in.eof()){
+ in >> name >> tax;
+ m->gobble(in);
+
+ //are there confidence scores, if so remove them
+ if (tax.find_first_of('(') != -1) { m->removeConfidences(tax); }
+
+ taxMap[name] = tax;
+
+ if (m->control_pressed) { in.close(); taxMap.clear(); return 0; }
+ }
+ in.close();
+
+ return 0;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClassifyOtuCommand", "readTaxonomyFile");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+vector<string> ClassifyOtuCommand::findConsensusTaxonomy(int bin, ListVector* thisList, int& size, string& conTax) {
+ try{
+ conTax = "";
+ vector<string> names;
+ vector<string> allNames;
+ map<string, string>::iterator it;
+ map<string, string>::iterator it2;
+
+ //parse names into vector
+ string binnames = thisList->get(bin);
+ m->splitAtComma(binnames, names);
+
+ //create a tree containing sequences from this bin
+ PhyloTree* phylo = new PhyloTree();
+
+ size = 0;
+ for (int i = 0; i < names.size(); i++) {
+
+ //if namesfile include the names
+ if (namefile != "") {
+
+ //is this sequence in the name file - namemap maps seqName -> repSeqName
+ it2 = nameMap.find(names[i]);
+
+ if (it2 == nameMap.end()) { //this name is not in name file, skip it
+ m->mothurOut(names[i] + " is not in your name file. I will not include it in the consensus."); m->mothurOutEndLine();
+ }else{
+
+ //is this sequence in the taxonomy file - look for repSeqName since we are assuming the taxonomy file is unique
+ it = taxMap.find(it2->second);
+
+ if (it == taxMap.end()) { //this name is not in taxonomy file, skip it
+
+ if (names[i] != it2->second) { m->mothurOut(names[i] + " is represented by " + it2->second + " and is not in your taxonomy file. I will not include it in the consensus."); m->mothurOutEndLine(); }
+ else { m->mothurOut(names[i] + " is not in your taxonomy file. I will not include it in the consensus."); m->mothurOutEndLine(); }
+ }else{
+
+ //add seq to tree
+ phylo->addSeqToTree(names[i], it->second);
+ size++;
+ allNames.push_back(names[i]);
+ }
+ }
+
+ }else{
+ //is this sequence in the taxonomy file - look for repSeqName since we are assuming the taxonomy file is unique
+ it = taxMap.find(names[i]);
+
+ if (it == taxMap.end()) { //this name is not in taxonomy file, skip it
+ m->mothurOut(names[i] + " is not in your taxonomy file. I will not include it in the consensus."); m->mothurOutEndLine();
+ }else{
+ //add seq to tree
+ phylo->addSeqToTree(names[i], it->second);
+ size++;
+ allNames.push_back(names[i]);
+ }
+ }
+
+
+ if (m->control_pressed) { delete phylo; return allNames; }
+
+ }
+
+ //build tree
+ phylo->assignHeirarchyIDs(0);
+
+ TaxNode currentNode = phylo->get(0);
+ int myLevel = 0;
+ //at each level
+ while (currentNode.children.size() != 0) { //you still have more to explore
+
+ TaxNode bestChild;
+ int bestChildSize = 0;
+
+ //go through children
+ for (map<string, int>::iterator itChild = currentNode.children.begin(); itChild != currentNode.children.end(); itChild++) {
+
+ TaxNode temp = phylo->get(itChild->second);
+
+ //select child with largest accesions - most seqs assigned to it
+ if (temp.accessions.size() > bestChildSize) {
+ bestChild = phylo->get(itChild->second);
+ bestChildSize = temp.accessions.size();
+ }
+
+ }
+
+ //is this taxonomy above cutoff
+ int consensusConfidence = ceil((bestChildSize / (float) size) * 100);
+
+ if (consensusConfidence >= cutoff) { //if yes, add it
+ if (probs) {
+ conTax += bestChild.name + "(" + toString(consensusConfidence) + ");";
+ }else{
+ conTax += bestChild.name + ";";
+ }
+ myLevel++;
+ }else{ //if no, quit
+ break;
+ }
+
+ //move down a level
+ currentNode = bestChild;
+ }
+
+ if (myLevel != phylo->getMaxLevel()) {
+ while (myLevel != phylo->getMaxLevel()) {
+ conTax += "unclassified;";
+ myLevel++;
+ }
+ }
+ if (conTax == "") { conTax = "no_consensus;"; }
+
+ delete phylo;
+
+ return allNames;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClassifyOtuCommand", "findConsensusTaxonomy");
+ exit(1);
+ }
+}
+
+//**********************************************************************************************************************
+int ClassifyOtuCommand::process(ListVector* processList) {
+ try{
+ string conTax;
+ int size;
+
+ //create output file
+ if (outputDir == "") { outputDir += m->hasPath(listfile); }
+
+ ofstream out;
+ string outputFile = outputDir + m->getRootName(m->getSimpleName(listfile)) + processList->getLabel() + ".cons.taxonomy";
+ m->openOutputFile(outputFile, out);
+ outputNames.push_back(outputFile); outputTypes["constaxonomy"].push_back(outputFile);
+
+ ofstream outSum;
+ string outputSumFile = outputDir + m->getRootName(m->getSimpleName(listfile)) + processList->getLabel() + ".cons.tax.summary";
+ m->openOutputFile(outputSumFile, outSum);
+ outputNames.push_back(outputSumFile); outputTypes["taxsummary"].push_back(outputSumFile);
+
+ out << "OTU\tSize\tTaxonomy" << endl;
+
+ PhyloSummary* taxaSum;
+ if (refTaxonomy != "") {
+ taxaSum = new PhyloSummary(refTaxonomy, groupfile);
+ }else {
+ taxaSum = new PhyloSummary(groupfile);
+ }
+
+ //for each bin in the list vector
+ for (int i = 0; i < processList->getNumBins(); i++) {
+
+ if (m->control_pressed) { break; }
+
+ vector<string> names;
+ names = findConsensusTaxonomy(i, processList, size, conTax);
+
+ if (m->control_pressed) { out.close(); return 0; }
+
+ //output to new names file
+ out << (i+1) << '\t' << size << '\t' << conTax << endl;
+
+ string noConfidenceConTax = conTax;
+ m->removeConfidences(noConfidenceConTax);
+
+ //add this bins taxonomy to summary
+ if (basis == "sequence") {
+ for(int j = 0; j < names.size(); j++) { taxaSum->addSeqToTree(names[j], noConfidenceConTax); }
+ }else { //otu
+ taxaSum->addSeqToTree(noConfidenceConTax, names);
+ }
+ }
+
+ out.close();
+
+ //print summary file
+ taxaSum->print(outSum);
+ outSum.close();
+
+ delete taxaSum;
+
+ return 0;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClassifyOtuCommand", "process");
+ exit(1);
+ }
+}
+/**************************************************************************************************/
+string ClassifyOtuCommand::addUnclassifieds(string tax, int maxlevel) {
+ try{
+ string newTax, taxon;
+ int level = 0;
+
+ //keep what you have counting the levels
+ while (tax.find_first_of(';') != -1) {
+ //get taxon
+ taxon = tax.substr(0,tax.find_first_of(';'))+';';
+ tax = tax.substr(tax.find_first_of(';')+1, tax.length());
+ newTax += taxon;
+ level++;
+ }
+
+ //add "unclassified" until you reach maxLevel
+ while (level < maxlevel) {
+ newTax += "unclassified;";
+ level++;
+ }
+
+ return newTax;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClassifyOtuCommand", "addUnclassifieds");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+
+
projects / mothur.git / blobdiff