5 * Created by Sarah Westcott on 1/22/09.
6 * Copyright 2009 Schloss Lab UMASS Amherst. All rights reserved.
13 /*****************************************************************/
17 globaldata = GlobalData::getInstance();
18 numLeaves = globaldata->gTreemap->getNumSeqs();
19 numNodes = 2*numLeaves - 1;
21 tree.resize(numNodes);
23 //initialize tree with correct number of nodes, name and group info.
24 for (int i = 0; i < numNodes; i++) {
25 //initialize leaf nodes
26 if (i <= (numLeaves-1)) {
27 tree[i].setName(globaldata->gTreemap->namesOfSeqs[i]);
28 tree[i].setGroup(globaldata->gTreemap->getGroup(globaldata->gTreemap->namesOfSeqs[i]));
29 //the node knows its index
31 //set pcount and pGroup for groupname to 1.
32 tree[i].pcount[globaldata->gTreemap->getGroup(globaldata->gTreemap->namesOfSeqs[i])] = 1;
33 tree[i].pGroups[globaldata->gTreemap->getGroup(globaldata->gTreemap->namesOfSeqs[i])] = 1;
34 //Treemap knows name, group and index to speed up search
35 globaldata->gTreemap->setIndex(globaldata->gTreemap->namesOfSeqs[i], i);
37 //intialize non leaf nodes
38 }else if (i > (numLeaves-1)) {
41 //the node knows its index
47 cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function Tree. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
51 cout << "An unknown error has occurred in the Tree class function Tree. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
56 /*****************************************************************/
58 int Tree::getIndex(string searchName) {
60 //Treemap knows name, group and index to speed up search
61 // getIndex function will return the vector index or -1 if seq is not found.
62 int index = globaldata->gTreemap->getIndex(searchName);
67 cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function getIndex. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
71 cout << "An unknown error has occurred in the Tree class function getIndex. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
75 /*****************************************************************/
77 void Tree::setIndex(string searchName, int index) {
79 //set index in treemap
80 globaldata->gTreemap->setIndex(searchName, index);
83 cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function setIndex. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
87 cout << "An unknown error has occurred in the Tree class function setIndex. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
91 /*****************************************************************/
92 void Tree::assembleTree() {
94 //build the pGroups in non leaf nodes to be used in the parsimony calcs.
95 for (int i = numLeaves; i < numNodes; i++) {
96 tree[i].pGroups = (mergeGroups(i));
97 tree[i].pcount = (mergeGcounts(i));
100 catch(exception& e) {
101 cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function assembleTree. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
105 cout << "An unknown error has occurred in the Tree class function assembleTree. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
109 /*****************************************************************/
110 void Tree::getCopy(Tree* copy) {
113 //for each node in the tree copy its info
114 for (int i = 0; i < numNodes; i++) {
116 tree[i].setName(copy->tree[i].getName());
119 tree[i].setGroup(copy->tree[i].getGroup());
122 tree[i].setBranchLength(copy->tree[i].getBranchLength());
125 tree[i].setParent(copy->tree[i].getParent());
128 tree[i].setChildren(copy->tree[i].getLChild(), copy->tree[i].getRChild());
130 //copy index in node and tmap
131 tree[i].setIndex(copy->tree[i].getIndex());
132 setIndex(copy->tree[i].getName(), getIndex(copy->tree[i].getName()));
135 tree[i].pGroups = copy->tree[i].pGroups;
138 tree[i].pcount = copy->tree[i].pcount;
141 catch(exception& e) {
142 cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function getCopy. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
146 cout << "An unknown error has occurred in the Tree class function getCopy. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
150 /*****************************************************************/
151 //returns a map with a groupname and the number of times that group was seen in the children
152 //for instance if your children are white and black then it would return a map with 2 entries
153 // p[white] = 1 and p[black] = 1. Now go up a level and merge that with a node who has p[white] = 1
154 //and you get p[white] = 2, p[black] = 1, but you erase the p[black] because you have a p value higher than 1.
156 map<string, int> Tree::mergeGroups(int i) {
159 int lc = tree[i].getLChild();
160 int rc = tree[i].getRChild();
162 //set parsimony groups to left child
163 map<string,int> parsimony = tree[lc].pGroups;
167 //look at right child groups and update maxPars if right child has something higher for that group.
168 for(it=tree[rc].pGroups.begin();it!=tree[rc].pGroups.end();it++){
169 parsimony[it->first]++;
171 if(parsimony[it->first] > maxPars){
172 maxPars = parsimony[it->first];
176 // this is true if right child had a greater parsimony for a certain group
178 //erase all the groups that are only 1 because you found something with 2.
179 for(it=parsimony.begin();it!=parsimony.end();it++){
181 parsimony.erase(it->first);
185 //set one remaining groups to 1
186 //so with our above example p[white] = 2 would be left and it would become p[white] = 1
187 for(it=parsimony.begin();it!=parsimony.end();it++){
188 parsimony[it->first] = 1;
195 catch(exception& e) {
196 cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function mergeGroups. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
200 cout << "An unknown error has occurred in the Tree class function mergeGroups. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
205 /**************************************************************************************************/
207 map<string,int> Tree::mergeGcounts(int position) {
209 map<string,int>::iterator pos;
211 int lc = tree[position].getLChild();
212 int rc = tree[position].getRChild();
214 map<string,int> sum = tree[lc].pcount;
216 for(it=tree[rc].pcount.begin();it!=tree[rc].pcount.end();it++){
217 sum[it->first] += it->second;
221 catch(exception& e) {
222 cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function mergeGcounts. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
226 cout << "An unknown error has occurred in the Tree class function mergeGcounts. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
230 /**************************************************************************************************/
232 void Tree::randomLabels() {
235 //set up the groups the user wants to include
238 for(int i=numLeaves-1;i>=0;i--){
239 if(tree[i].pGroups.size() == 0){
247 //get random index to switch with
248 z = int((float)(i+1) * (float)(rand()) / ((float)RAND_MAX+1.0));
250 if(tree[z].pGroups.size() != 0){
255 //you only want to randomize the nodes that are from a group the user wants analyzed, so
256 //if either of the leaf nodes you are about to switch are not in the users groups then you don't want to switch them.
259 //leaves have only one group so you can just set it to begin()
260 it = tree[z].pGroups.begin();
261 treez = inUsersGroups(it->first, globaldata->Groups);
263 it = tree[i].pGroups.begin();
264 treei = inUsersGroups(it->first, globaldata->Groups);
266 if ((treez == true) && (treei == true)) {
267 //switches node i and node z's info.
268 map<string,int> lib_hold = tree[z].pGroups;
269 tree[z].pGroups = (tree[i].pGroups);
270 tree[i].pGroups = (lib_hold);
272 tree[z].setGroup(tree[z].pGroups.begin()->first);
273 tree[i].setGroup(tree[i].pGroups.begin()->first);
275 map<string,int> gcount_hold = tree[z].pcount;
276 tree[z].pcount = (tree[i].pcount);
277 tree[i].pcount = (gcount_hold);
281 catch(exception& e) {
282 cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function randomLabels. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
286 cout << "An unknown error has occurred in the Tree class function randomLabels. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
290 /**************************************************************************************************/
292 void Tree::randomBlengths() {
294 for(int i=numNodes-1;i>=0;i--){
295 int z = int((float)(i+1) * (float)(rand()) / ((float)RAND_MAX+1.0));
297 float bl_hold = tree[z].getBranchLength();
298 tree[z].setBranchLength(tree[i].getBranchLength());
299 tree[i].setBranchLength(bl_hold);
302 catch(exception& e) {
303 cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function randomBlengths. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
307 cout << "An unknown error has occurred in the Tree class function randomBlengths. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
311 /*************************************************************************************************/
312 void Tree::assembleRandomUnifracTree() {
317 /*************************************************************************************************/
318 //for now it's just random topology but may become random labels as well later that why this is such a simple function now...
319 void Tree::assembleRandomTree() {
323 /**************************************************************************************************/
325 void Tree::randomTopology() {
327 for(int i=0;i<numNodes;i++){
328 tree[i].setParent(-1);
330 for(int i=numLeaves;i<numNodes;i++){
331 tree[i].setChildren(-1, -1);
334 for(int i=numLeaves;i<numNodes;i++){
336 int rnd_index1, rnd_index2;
338 rnd_index1 = (int)(((double)rand() / (double) RAND_MAX)*i);
339 if(tree[rnd_index1].getParent() == -1){escape = 1;}
344 rnd_index2 = (int)(((double)rand() / (double) RAND_MAX)*i);
345 if(rnd_index2 != rnd_index1 && tree[rnd_index2].getParent() == -1){
350 tree[i].setChildren(rnd_index1,rnd_index2);
351 tree[i].setParent(-1);
352 tree[rnd_index1].setParent(i);
353 tree[rnd_index2].setParent(i);
356 catch(exception& e) {
357 cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function randomTopology. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
361 cout << "An unknown error has occurred in the Tree class function randomTopology. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
366 /*****************************************************************/
367 // This prints out the tree in Newick form.
368 void Tree::createNewickFile() {
370 int root = findRoot();
371 filename = getRootName(globaldata->getTreeFile()) + "newick";
372 openOutputFile(filename, out);
376 // you are at the end of the tree
379 catch(exception& e) {
380 cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function createNewickFile. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
384 cout << "An unknown error has occurred in the Tree class function createNewickFile. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
389 /*****************************************************************/
390 //This function finds the index of the root node.
392 int Tree::findRoot() {
394 for (int i = 0; i < numNodes; i++) {
396 if (tree[i].getParent() == -1) { return i; }
400 catch(exception& e) {
401 cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function findRoot. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
405 cout << "An unknown error has occurred in the Tree class function findRoot. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
410 /*****************************************************************/
411 void Tree::printBranch(int node) {
414 // you are not a leaf
415 if (tree[node].getLChild() != -1) {
417 printBranch(tree[node].getLChild());
419 printBranch(tree[node].getRChild());
421 }else { //you are a leaf
422 tree[node].printNode(out); //prints out name and branch length
426 catch(exception& e) {
427 cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function printBranch. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
431 cout << "An unknown error has occurred in the Tree class function printBranch. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
436 /*****************************************************************/
437 void Tree::setGroups() {
439 //if the user has not entered specific groups to analyze then do them all
440 if (globaldata->Groups.size() != 0) {
441 //check that groups are valid
442 for (int i = 0; i < globaldata->Groups.size(); i++) {
443 if (globaldata->gTreemap->isValidGroup(globaldata->Groups[i]) != true) {
444 cout << globaldata->Groups[i] << " is not a valid group, and will be disregarded." << endl;
445 // erase the invalid group from globaldata->Groups
446 globaldata->Groups.erase (globaldata->Groups.begin()+i);
450 //if the user only entered invalid groups
451 if (globaldata->Groups.size() == 0) {
452 cout << "When using the groups parameter you must have at least 1 valid group. I will run the command using all the groups in your groupfile." << endl;
453 for (int i = 0; i < globaldata->gTreemap->namesOfGroups.size(); i++) {
454 globaldata->Groups.push_back(globaldata->gTreemap->namesOfGroups[i]);
459 for (int i = 0; i < globaldata->gTreemap->namesOfGroups.size(); i++) {
460 globaldata->Groups.push_back(globaldata->gTreemap->namesOfGroups[i]);
464 catch(exception& e) {
465 cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function setGroups. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
469 cout << "An unknown error has occurred in the Tree class function setGroups. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";