splitmatrix.cpp

   1 /*
   2  *  splitmatrix.cpp
   3  *  Mothur
   4  *
   5  *  Created by westcott on 5/19/10.
   6  *  Copyright 2010 Schloss Lab. All rights reserved.
   7  *
   8  */
   9
  10 #include "splitmatrix.h"
  11 #include "phylotree.h"
  12
  13 /***********************************************************************/
  14
  15 SplitMatrix::SplitMatrix(string distfile, string name, string tax, float c, string t){
  16         m = MothurOut::getInstance();
  17         distFile = distfile;
  18         cutoff = c;
  19         namefile = name;
  20         method = t;
  21         taxFile = tax;
  22 }
  23
  24 /***********************************************************************/
  25
  26 int SplitMatrix::split(){
  27         try {
  28
  29                 if (method == "distance") {
  30                         splitDistance();
  31                 }else if (method == "classify") {
  32                         splitClassify();
  33                 }else {
  34                         m->mothurOut("Unknown splitting method, aborting split."); m->mothurOutEndLine();
  35                         map<string, string> temp;
  36                         temp[distFile] = namefile;
  37                         dists.push_back(temp);
  38                 }
  39
  40                 return 0;
  41         }
  42         catch(exception& e) {
  43                 m->errorOut(e, "SplitMatrix", "split");
  44                 exit(1);
  45         }
  46 }
  47 /***********************************************************************/
  48 int SplitMatrix::splitDistance(){
  49         try {
  50
  51                 vector<set<string> > groups;
  52                 int numGroups = 0;
  53
  54                 ofstream outFile;
  55                 ifstream dFile;
  56                 openInputFile(distFile, dFile);
  57
  58                 while(dFile){
  59                         string seqA, seqB;
  60                         float dist;
  61
  62                         dFile >> seqA >> seqB >> dist;
  63
  64                         if (m->control_pressed) {  outFile.close(); dFile.close();  for(int i=0;i<numGroups;i++){       if(groups[i].size() > 0){  remove((distFile + "." + toString(i) + ".temp").c_str()); }  } return 0; }
  65
  66                         if(dist < cutoff){
  67                                 //cout << "in cutoff: " << dist << endl;
  68                                 int groupIDA = -1;
  69                                 int groupIDB = -1;
  70                                 int groupID = -1;
  71                                 int prevGroupID = -1;
  72
  73                                 for(int i=0;i<numGroups;i++){
  74                                         set<string>::iterator aIt = groups[i].find(seqA);
  75                                         set<string>::iterator bIt = groups[i].find(seqB);
  76
  77                                         if(groupIDA == -1 && aIt != groups[i].end()){//seqA is not already assigned to a group and is in group[i], so assign seqB to group[i]
  78                                                 groups[i].insert(seqB);
  79                                                 groupIDA = i;
  80                                                 groupID = groupIDA;
  81
  82                                                 //cout << "in aIt: " << groupID << endl;
  83         //                                      break;
  84                                         }
  85                                         else if(groupIDB == -1 && bIt != groups[i].end()){//seqB is not already assigned to a group and is in group[i], so assign seqA to group[i]
  86                                                 groups[i].insert(seqA);
  87                                                 groupIDB = i;
  88                                                 groupID = groupIDB;
  89
  90                                         //      cout << "in bIt: " << groupID << endl;
  91         //                                      break;
  92                                         }
  93
  94                                         if(groupIDA != -1 && groupIDB != -1){//both ifs above have been executed, so we need to decide who to assign them to
  95                                                 if(groupIDA < groupIDB){
  96                                                 //      cout << "A: " << groupIDA << "\t" << groupIDB << endl;
  97                                                         groups[groupIDA].insert(groups[groupIDB].begin(), groups[groupIDB].end()); //merge two groups into groupIDA
  98                                                         groups[groupIDB].clear();
  99                                                         groupID = groupIDA;
 100                                                 }
 101                                                 else{
 102                                                 //      cout << "B: " << groupIDA << "\t" << groupIDB << endl;
 103                                                         groups[groupIDB].insert(groups[groupIDA].begin(), groups[groupIDA].end()); //merge two groups into groupIDB
 104                                                         groups[groupIDA].clear();
 105                                                         groupID = groupIDB;
 106                                                 }
 107                                                 break;
 108                                         }
 109                                 }
 110
 111         //windows is gonna gag on the reuse of outFile, will need to make it local...
 112
 113                                 if(groupIDA == -1 && groupIDB == -1){ //we need a new group
 114                                         set<string> newGroup;
 115                                         newGroup.insert(seqA);
 116                                         newGroup.insert(seqB);
 117                                         groups.push_back(newGroup);
 118
 119                                         outFile.close();
 120                                         string fileName = distFile + "." + toString(numGroups) + ".temp";
 121                                         outFile.open(fileName.c_str(), ios::ate);
 122
 123                                         outFile << seqA << '\t' << seqB << '\t' << dist << endl;
 124                                         numGroups++;
 125                                 }
 126                                 else{
 127                                         string fileName = distFile + "." + toString(groupID) + ".temp";
 128                                         if(groupID != prevGroupID){
 129                                                 outFile.close();
 130                                                 outFile.open(fileName.c_str(), ios::app);
 131                                                 prevGroupID     = groupID;
 132                                         }
 133                                         outFile << seqA << '\t' << seqB << '\t' << dist << endl;
 134
 135                                         if(groupIDA != -1 && groupIDB != -1){ //merge distance files of two groups you merged above
 136                                                 string row, column, distance;
 137                                                 if(groupIDA<groupIDB){
 138                                                         string fileName = distFile + "." + toString(groupIDB) + ".temp";
 139                                                         ifstream fileB(fileName.c_str());
 140                                                         while(fileB){
 141                                                                 fileB >> row >> column >> distance;
 142                                                                 outFile << row << '\t' << column << '\t' << distance << endl;
 143                                                                 gobble(fileB);
 144                                                         }
 145                                                         fileB.close();
 146                                                         remove(fileName.c_str());
 147                                                 }
 148                                                 else{
 149                                                         string fileName = distFile + "." + toString(groupIDA) + ".temp";
 150                                                         ifstream fileA(fileName.c_str());
 151                                                         while(fileA){
 152                                                                 fileA >> row >> column >> distance;
 153                                                                 outFile << row << '\t' << column << '\t' << distance << endl;
 154                                                                 gobble(fileA);
 155                                                         }
 156                                                         fileA.close();
 157                                                         remove(fileName.c_str());
 158                                                 }
 159                                         }
 160                                 }
 161                         }
 162                         gobble(dFile);
 163                 }
 164                 outFile.close();
 165                 dFile.close();
 166
 167                 ifstream bigNameFile(namefile.c_str());
 168                 if(!bigNameFile){
 169                         cerr << "Error: We can't open the name file\n";
 170                         exit(1);
 171                 }
 172
 173                 map<string, string> nameMap;
 174                 string name, nameList;
 175                 while(bigNameFile){
 176                         bigNameFile >> name >> nameList;
 177                         nameMap[name] = nameList;
 178                         gobble(bigNameFile);
 179                 }
 180                 bigNameFile.close();
 181
 182                 for(int i=0;i<numGroups;i++){  //parse names file to match distance files
 183                         int numSeqsInGroup = groups[i].size();
 184
 185                         if(numSeqsInGroup > 0){
 186                                 string fileName = namefile + "." + toString(i) + ".temp";
 187                                 ofstream smallNameFile(fileName.c_str(), ios::ate);
 188
 189                                 for(set<string>::iterator gIt=groups[i].begin();gIt!=groups[i].end();gIt++){
 190                                         map<string,string>::iterator nIt = nameMap.find(*gIt);
 191
 192                                         if (nIt != nameMap.end()) {
 193                                                 smallNameFile << nIt->first << '\t' << nIt->second << endl;
 194                                                 nameMap.erase(nIt);
 195                                         }else{
 196                                                 m->mothurOut((*gIt) + " is in your distance file and not in your namefile.  Please correct."); m->mothurOutEndLine(); exit(1);
 197                                         }
 198                                 }
 199                                 smallNameFile.close();
 200                         }
 201                 }
 202
 203                 //names of singletons
 204                 if (nameMap.size() != 0) {
 205                         singleton = namefile + ".extra.temp";
 206                         ofstream remainingNames(singleton.c_str(), ios::ate);
 207                         for(map<string,string>::iterator nIt=nameMap.begin();nIt!=nameMap.end();nIt++){
 208                                 remainingNames << nIt->first << '\t' << nIt->second << endl;
 209                         }
 210                         remainingNames.close();
 211                 }else { singleton = "none"; }
 212
 213                 for(int i=0;i<numGroups;i++){
 214                         if(groups[i].size() > 0){
 215                                 string tempNameFile = namefile + "." + toString(i) + ".temp";
 216                                 string tempDistFile = distFile + "." + toString(i) + ".temp";
 217
 218                                 map<string, string> temp;
 219                                 temp[tempDistFile] = tempNameFile;
 220                                 dists.push_back(temp);
 221                         }
 222                 }
 223
 224                 if (m->control_pressed)  {
 225                         for (int i = 0; i < dists.size(); i++) {
 226                                 remove((dists[i].begin()->first).c_str());
 227                                 remove((dists[i].begin()->second).c_str());
 228                         }
 229                         dists.clear();
 230                 }
 231
 232                 return 0;
 233
 234         }
 235         catch(exception& e) {
 236                 m->errorOut(e, "SplitMatrix", "splitDistance");
 237                 exit(1);
 238         }
 239 }
 240
 241 /***********************************************************************/
 242 int SplitMatrix::splitClassify(){
 243         try {
 244                 map<string, int> seqGroup;
 245                 map<string, int>::iterator it;
 246                 map<string, int>::iterator it2;
 247
 248                 int numGroups = 0;
 249
 250                 //build tree from users taxonomy file
 251                 PhyloTree* phylo = new PhyloTree();
 252
 253                 ifstream in;
 254                 openInputFile(taxFile, in);
 255
 256                 //read in users taxonomy file and add sequences to tree
 257                 string seqname, tax;
 258                 while(!in.eof()){
 259                         in >> seqname >> tax; gobble(in);
 260
 261                         phylo->addSeqToTree(seqname, tax);
 262                 }
 263                 in.close();
 264
 265                 phylo->assignHeirarchyIDs(0);
 266
 267                 //make sure the cutoff is not greater than maxlevel
 268                 if (cutoff > phylo->getMaxLevel()) { m->mothurOut("splitcutoff is greater than the longest taxonomy, using " + toString(phylo->getMaxLevel())); m->mothurOutEndLine(); cutoff = phylo->getMaxLevel(); }
 269
 270                 //for each node in tree
 271                 for (int i = 0; i < phylo->getNumNodes(); i++) {
 272
 273                         //is this node within the cutoff
 274                         TaxNode taxon = phylo->get(i);
 275
 276                         if (taxon.level == cutoff) {//if yes, then create group containing this nodes sequences
 277                                 if (taxon.children.size() > 1) { //if this taxon just has one seq its a singleton
 278                                         for (it = taxon.children.begin(); it != taxon.children.end(); it++) {
 279                                                 seqGroup[it->first] = numGroups;
 280                                         }
 281                                         numGroups++;
 282                                 }
 283                         }
 284                 }
 285
 286                 ifstream dFile;
 287                 openInputFile(distFile, dFile);
 288                 ofstream outFile;
 289
 290                 for (int i = 0; i < numGroups; i++) { //remove old temp files, just in case
 291                         remove((distFile + "." + toString(i) + ".temp").c_str());
 292                 }
 293
 294                 //for each distance
 295                 while(dFile){
 296                         string seqA, seqB;
 297                         float dist;
 298
 299                         if (m->control_pressed) { dFile.close(); for (int i = 0; i < numGroups; i++) { remove((distFile + "." + toString(i) + ".temp").c_str());        } }
 300
 301                         dFile >> seqA >> seqB >> dist;  gobble(dFile);
 302
 303                         //if both sequences are in the same group then they are within the cutoff
 304                         it = seqGroup.find(seqA);
 305                         it2 = seqGroup.find(seqB);
 306
 307                         if ((it != seqGroup.end()) && (it2 != seqGroup.end())) { //they are both not singletons
 308                                 if (it->second == it2->second) { //they are from the same group so add the distance
 309                                         openOutputFileAppend((distFile + "." + toString(it->second) + ".temp"), outFile);
 310                                         outFile << seqA << '\t' << seqB << '\t' << dist << endl;
 311                                         outFile.close();
 312                                 }
 313                         }
 314                 }
 315                 dFile.close();
 316
 317
 318                 for (int i = 0; i < numGroups; i++) { //remove old temp files, just in case
 319                         remove((namefile + "." + toString(i) + ".temp").c_str());
 320                 }
 321
 322                 ifstream bigNameFile;
 323                 openInputFile(namefile, bigNameFile);
 324
 325                 singleton = namefile + ".extra.temp";
 326                 ofstream remainingNames;
 327                 openOutputFile(singleton, remainingNames);
 328
 329                 bool wroteExtra = false;
 330
 331                 string name, nameList;
 332                 while(!bigNameFile.eof()){
 333                         bigNameFile >> name >> nameList;  gobble(bigNameFile);
 334
 335                         //did this sequence get assigned a group
 336                         it = seqGroup.find(name);
 337
 338                         if (it != seqGroup.end()) {
 339                                 openOutputFileAppend((namefile + "." + toString(it->second) + ".temp"), outFile);
 340                                 outFile << name << '\t' << nameList << endl;
 341                                 outFile.close();
 342                         }else{
 343                                 wroteExtra = true;
 344                                 remainingNames << name << '\t' << nameList << endl;
 345                         }
 346                 }
 347                 bigNameFile.close();
 348                 remainingNames.close();
 349
 350                 if (!wroteExtra) {
 351                         remove(singleton.c_str());
 352                         singleton = "none";
 353                 }
 354
 355                 for(int i=0;i<numGroups;i++){
 356                         string tempNameFile = namefile + "." + toString(i) + ".temp";
 357                         string tempDistFile = distFile + "." + toString(i) + ".temp";
 358
 359                         map<string, string> temp;
 360                         temp[tempDistFile] = tempNameFile;
 361                         dists.push_back(temp);
 362                 }
 363
 364                 if (m->control_pressed)  {
 365                         for (int i = 0; i < dists.size(); i++) {
 366                                 remove((dists[i].begin()->first).c_str());
 367                                 remove((dists[i].begin()->second).c_str());
 368                         }
 369                         dists.clear();
 370                 }
 371
 372                 return 0;
 373
 374         }
 375         catch(exception& e) {
 376                 m->errorOut(e, "SplitMatrix", "splitClassify");
 377                 exit(1);
 378         }
 379 }
 380 //********************************************************************************************************************
 381 //sorts biggest to smallest
 382 inline bool compareFileSizes(map<string, string> left, map<string, string> right){
 383
 384         FILE * pFile;
 385         long leftsize = 0;
 386
 387         //get num bytes in file
 388         string filename = left.begin()->first;
 389         pFile = fopen (filename.c_str(),"rb");
 390         string error = "Error opening " + filename;
 391         if (pFile==NULL) perror (error.c_str());
 392         else{
 393                 fseek (pFile, 0, SEEK_END);
 394                 leftsize=ftell (pFile);
 395                 fclose (pFile);
 396         }
 397
 398         FILE * pFile2;
 399         long rightsize = 0;
 400
 401         //get num bytes in file
 402         filename = right.begin()->first;
 403         pFile2 = fopen (filename.c_str(),"rb");
 404         error = "Error opening " + filename;
 405         if (pFile2==NULL) perror (error.c_str());
 406         else{
 407                 fseek (pFile2, 0, SEEK_END);
 408                 rightsize=ftell (pFile2);
 409                 fclose (pFile2);
 410         }
 411
 412         return (leftsize > rightsize);
 413 }
 414 /***********************************************************************/
 415 //returns map of distance files -> namefile sorted by distance file size
 416 vector< map< string, string> > SplitMatrix::getDistanceFiles(){
 417         try {
 418
 419                 sort(dists.begin(), dists.end(), compareFileSizes);
 420
 421                 return dists;
 422         }
 423         catch(exception& e) {
 424                 m->errorOut(e, "SplitMatrix", "getDistanceFiles");
 425                 exit(1);
 426         }
 427 }
 428 /***********************************************************************/
 429 SplitMatrix::~SplitMatrix(){}
 430 /***********************************************************************/
 431