splitmatrix.cpp

   1 /*
   2  *  splitmatrix.cpp
   3  *  Mothur
   4  *
   5  *  Created by westcott on 5/19/10.
   6  *  Copyright 2010 Schloss Lab. All rights reserved.
   7  *
   8  */
   9
  10 #include "splitmatrix.h"
  11 #include "phylotree.h"
  12 #include "distancecommand.h"
  13 #include "seqsummarycommand.h"
  14
  15 /***********************************************************************/
  16
  17 SplitMatrix::SplitMatrix(string distfile, string name, string count, string tax, float c, string t, bool l){
  18         m = MothurOut::getInstance();
  19         distFile = distfile;
  20         cutoff = c;
  21         namefile = name;
  22         method = t;
  23         taxFile = tax;
  24     countfile = count;
  25         large = l;
  26 }
  27 /***********************************************************************/
  28
  29 SplitMatrix::SplitMatrix(string ffile, string name, string count, string tax, float c, float cu, string t, int p, bool cl, string output){
  30         m = MothurOut::getInstance();
  31         fastafile = ffile;
  32         namefile = name;
  33     countfile = count;
  34         taxFile = tax;
  35         cutoff = c;  //tax level cutoff
  36         distCutoff = cu; //for fasta method if you are creating distance matrix you need a cutoff for that
  37         method = t;
  38         processors = p;
  39     classic = cl;
  40         outputDir = output;
  41 }
  42
  43 /***********************************************************************/
  44
  45 int SplitMatrix::split(){
  46         try {
  47
  48                 if (method == "distance") {
  49                         splitDistance();
  50                 }else if ((method == "classify") || (method == "fasta")) {
  51                         splitClassify();
  52                 }else {
  53                         m->mothurOut("Unknown splitting method, aborting split."); m->mothurOutEndLine();
  54                         map<string, string> temp;
  55                         if (namefile != "") {  temp[distFile] = namefile; }
  56             else { temp[distFile] = countfile; }
  57                         dists.push_back(temp);
  58                 }
  59
  60                 return 0;
  61         }
  62         catch(exception& e) {
  63                 m->errorOut(e, "SplitMatrix", "split");
  64                 exit(1);
  65         }
  66 }
  67 /***********************************************************************/
  68 int SplitMatrix::splitDistance(){
  69         try {
  70
  71                 if (large)      { splitDistanceLarge(); }
  72                 else            { splitDistanceRAM();   }
  73
  74                 return 0;
  75
  76         }
  77         catch(exception& e) {
  78                 m->errorOut(e, "SplitMatrix", "splitDistance");
  79                 exit(1);
  80         }
  81 }
  82
  83 /***********************************************************************/
  84 int SplitMatrix::splitClassify(){
  85         try {
  86                 cutoff = int(cutoff);
  87
  88                 map<string, int> seqGroup;
  89                 map<string, int>::iterator it;
  90                 map<string, int>::iterator it2;
  91
  92                 int numGroups = 0;
  93
  94                 //build tree from users taxonomy file
  95                 PhyloTree* phylo = new PhyloTree();
  96
  97         map<string, string> temp;
  98         m->readTax(taxFile, temp);
  99
 100         for (map<string, string>::iterator itTemp = temp.begin(); itTemp != temp.end();) {
 101             phylo->addSeqToTree(itTemp->first, itTemp->second);
 102             temp.erase(itTemp++);
 103         }
 104
 105                 phylo->assignHeirarchyIDs(0);
 106
 107                 //make sure the cutoff is not greater than maxlevel
 108                 if (cutoff > phylo->getMaxLevel()) { m->mothurOut("splitcutoff is greater than the longest taxonomy, using " + toString(phylo->getMaxLevel())); m->mothurOutEndLine(); cutoff = phylo->getMaxLevel(); }
 109
 110                 //for each node in tree
 111                 for (int i = 0; i < phylo->getNumNodes(); i++) {
 112
 113                         //is this node within the cutoff
 114                         TaxNode taxon = phylo->get(i);
 115
 116                         if (taxon.level == cutoff) {//if yes, then create group containing this nodes sequences
 117                                 if (taxon.accessions.size() > 1) { //if this taxon just has one seq its a singleton
 118                                         for (int j = 0; j < taxon.accessions.size(); j++) {
 119                                                 seqGroup[taxon.accessions[j]] = numGroups;
 120                                         }
 121                                         numGroups++;
 122                                 }
 123                         }
 124                 }
 125
 126                 delete phylo;
 127
 128                 if (method == "classify") {
 129                         splitDistanceFileByTax(seqGroup, numGroups);
 130                 }else {
 131                         createDistanceFilesFromTax(seqGroup, numGroups);
 132                 }
 133
 134                 return 0;
 135
 136         }
 137         catch(exception& e) {
 138                 m->errorOut(e, "SplitMatrix", "splitClassify");
 139                 exit(1);
 140         }
 141 }
 142 /***********************************************************************/
 143 int SplitMatrix::createDistanceFilesFromTax(map<string, int>& seqGroup, int numGroups){
 144         try {
 145                 map<string, int> copyGroups = seqGroup;
 146                 map<string, int>::iterator it;
 147                 set<string> names;
 148
 149                 for (int i = 0; i < numGroups; i++) { //remove old temp files, just in case
 150                         m->mothurRemove((fastafile + "." + toString(i) + ".temp"));
 151                 }
 152
 153                 ifstream in;
 154                 m->openInputFile(fastafile, in);
 155
 156                 //parse fastafile
 157                 ofstream outFile;
 158                 while (!in.eof()) {
 159                         Sequence query(in); m->gobble(in);
 160                         if (query.getName() != "") {
 161
 162                                 it = seqGroup.find(query.getName());
 163
 164                                 //save names in case no namefile is given
 165                                 if ((namefile == "") && (countfile == "")) {  names.insert(query.getName()); }
 166
 167                                 if (it != seqGroup.end()) { //not singleton
 168                                         m->openOutputFileAppend((fastafile + "." + toString(it->second) + ".temp"), outFile);
 169                                         query.printSequence(outFile);
 170                                         outFile.close();
 171
 172                                         copyGroups.erase(query.getName());
 173                                 }
 174                         }
 175                 }
 176                 in.close();
 177
 178                 //warn about sequence in groups that are not in fasta file
 179                 for(it = copyGroups.begin(); it != copyGroups.end(); it++) {
 180                         m->mothurOut("ERROR: " + it->first + " is missing from your fastafile. This could happen if your taxonomy file is not unique and your fastafile is, or it could indicate and error."); m->mothurOutEndLine();
 181                         exit(1);
 182                 }
 183
 184                 copyGroups.clear();
 185
 186                 //process each distance file
 187                 for (int i = 0; i < numGroups; i++) {
 188
 189                         string options = "";
 190             if (classic) { options = "fasta=" + (fastafile + "." + toString(i) + ".temp") + ", processors=" + toString(processors) + ", output=lt"; }
 191             else { options = "fasta=" + (fastafile + "." + toString(i) + ".temp") + ", processors=" + toString(processors) + ", cutoff=" + toString(distCutoff); }
 192                         if (outputDir != "") { options += ", outputdir=" + outputDir; }
 193
 194             m->mothurOut("/******************************************/"); m->mothurOutEndLine();
 195
 196                         Command* command = new DistanceCommand(options);
 197
 198             m->mothurOut("/******************************************/"); m->mothurOutEndLine();
 199
 200                         command->execute();
 201                         delete command;
 202
 203                         m->mothurRemove((fastafile + "." + toString(i) + ".temp"));
 204
 205                         //remove old names files just in case
 206                         if (namefile != "") { m->mothurRemove((namefile + "." + toString(i) + ".temp")); }
 207             else { m->mothurRemove((countfile + "." + toString(i) + ".temp")); }
 208                 }
 209
 210         vector<string> tempDistFiles;
 211         for(int i=0;i<numGroups;i++){
 212             if (outputDir == "") { outputDir = m->hasPath(fastafile); }
 213             string tempDistFile = "";
 214             if (classic) { tempDistFile =  outputDir + m->getRootName(m->getSimpleName((fastafile + "." + toString(i) + ".temp"))) + "phylip.dist";}
 215             else { tempDistFile = outputDir + m->getRootName(m->getSimpleName((fastafile + "." + toString(i) + ".temp"))) + "dist"; }
 216             tempDistFiles.push_back(tempDistFile);
 217         }
 218
 219         splitNames(seqGroup, numGroups, tempDistFiles);
 220
 221                 if (m->control_pressed)  {  for (int i = 0; i < dists.size(); i++) { m->mothurRemove((dists[i].begin()->first)); m->mothurRemove((dists[i].begin()->second)); } dists.clear(); }
 222
 223                 return 0;
 224         }
 225         catch(exception& e) {
 226                 m->errorOut(e, "SplitMatrix", "createDistanceFilesFromTax");
 227                 exit(1);
 228         }
 229 }
 230 /***********************************************************************/
 231 int SplitMatrix::splitDistanceFileByTax(map<string, int>& seqGroup, int numGroups){
 232         try {
 233                 map<string, int>::iterator it;
 234                 map<string, int>::iterator it2;
 235
 236         ofstream outFile;
 237                 ifstream dFile;
 238                 m->openInputFile(distFile, dFile);
 239
 240
 241                 for (int i = 0; i < numGroups; i++) { //remove old temp files, just in case
 242                         m->mothurRemove((distFile + "." + toString(i) + ".temp"));
 243                 }
 244
 245                 //for buffering the io to improve speed
 246                  //allow for 10 dists to be stored, then output.
 247                 vector<string> outputs;  outputs.resize(numGroups, "");
 248                 vector<int> numOutputs;  numOutputs.resize(numGroups, 0);
 249
 250                 //you can have a group made, but their may be no distances in the file for this group if the taxonomy file and distance file don't match
 251                 //this can occur if we have converted the phylip to column, since we reduce the size at that step by using the cutoff value
 252                 vector<bool> validDistances;   validDistances.resize(numGroups, false);
 253
 254                 //for each distance
 255                 while(dFile){
 256                         string seqA, seqB;
 257                         float dist;
 258
 259                         if (m->control_pressed) { dFile.close(); for (int i = 0; i < numGroups; i++) { m->mothurRemove((distFile + "." + toString(i) + ".temp"));       } }
 260
 261                         dFile >> seqA >> seqB >> dist;  m->gobble(dFile);
 262
 263                         //if both sequences are in the same group then they are within the cutoff
 264                         it = seqGroup.find(seqA);
 265                         it2 = seqGroup.find(seqB);
 266
 267                         if ((it != seqGroup.end()) && (it2 != seqGroup.end())) { //they are both not singletons
 268                                 if (it->second == it2->second) { //they are from the same group so add the distance
 269                                         if (numOutputs[it->second] > 30) {
 270                                                 m->openOutputFileAppend((distFile + "." + toString(it->second) + ".temp"), outFile);
 271                                                 outFile << outputs[it->second] << seqA << '\t' << seqB << '\t' << dist << endl;
 272                                                 outFile.close();
 273                                                 outputs[it->second] = "";
 274                                                 numOutputs[it->second] = 0;
 275                                                 validDistances[it->second] = true;
 276                                         }else{
 277                                                 outputs[it->second] += seqA + '\t' + seqB + '\t' + toString(dist)  + '\n';
 278                                                 numOutputs[it->second]++;
 279                                         }
 280                                 }
 281                         }
 282                 }
 283                 dFile.close();
 284
 285         string inputFile = namefile;
 286         if (countfile != "") { inputFile = countfile; }
 287
 288         vector<string> tempDistFiles;
 289                 for (int i = 0; i < numGroups; i++) { //remove old temp files, just in case
 290             string tempDistFile = distFile + "." + toString(i) + ".temp";
 291             tempDistFiles.push_back(tempDistFile);
 292                         m->mothurRemove((inputFile + "." + toString(i) + ".temp"));
 293
 294                         //write out any remaining buffers
 295                         if (numOutputs[i] > 0) {
 296                                 m->openOutputFileAppend((distFile + "." + toString(i) + ".temp"), outFile);
 297                                 outFile << outputs[i];
 298                                 outFile.close();
 299                                 outputs[i] = "";
 300                                 numOutputs[i] = 0;
 301                                 validDistances[i] = true;
 302                         }
 303                 }
 304
 305         splitNames(seqGroup, numGroups, tempDistFiles);
 306
 307                 if (m->control_pressed)  {
 308                         for (int i = 0; i < dists.size(); i++) {
 309                                 m->mothurRemove((dists[i].begin()->first));
 310                                 m->mothurRemove((dists[i].begin()->second));
 311                         }
 312                         dists.clear();
 313                 }
 314
 315                 return 0;
 316         }
 317         catch(exception& e) {
 318                 m->errorOut(e, "SplitMatrix", "splitDistanceFileByTax");
 319                 exit(1);
 320         }
 321 }
 322 /***********************************************************************/
 323 int SplitMatrix::splitDistanceLarge(){
 324         try {
 325                 vector<set<string> > groups;
 326
 327                 //for buffering the io to improve speed
 328                  //allow for 30 dists to be stored, then output.
 329                 vector<string> outputs;
 330                 vector<int> numOutputs;
 331                 vector<bool> wroteOutPut;
 332
 333                 int numGroups = 0;
 334
 335                 ofstream outFile;
 336                 ifstream dFile;
 337                 m->openInputFile(distFile, dFile);
 338
 339                 while(dFile){
 340                         string seqA, seqB;
 341                         float dist;
 342
 343                         dFile >> seqA >> seqB >> dist;
 344
 345                         if (m->control_pressed) {   dFile.close();  for(int i=0;i<numGroups;i++){       if(groups[i].size() > 0){  m->mothurRemove((distFile + "." + toString(i) + ".temp")); }  } return 0; }
 346
 347                         if(dist < cutoff){
 348                                 //cout << "in cutoff: " << dist << endl;
 349                                 int groupIDA = -1;
 350                                 int groupIDB = -1;
 351                                 int groupID = -1;
 352
 353                                 for(int i=0;i<numGroups;i++){
 354                                         set<string>::iterator aIt = groups[i].find(seqA);
 355                                         set<string>::iterator bIt = groups[i].find(seqB);
 356
 357                                         if(groupIDA == -1 && aIt != groups[i].end()){//seqA is not already assigned to a group and is in group[i], so assign seqB to group[i]
 358                                                 groups[i].insert(seqB);
 359                                                 groupIDA = i;
 360                                                 groupID = groupIDA;
 361
 362                                                 //cout << "in aIt: " << groupID << endl;
 363         //                                      break;
 364                                         }
 365                                         else if(groupIDB == -1 && bIt != groups[i].end()){//seqB is not already assigned to a group and is in group[i], so assign seqA to group[i]
 366                                                 groups[i].insert(seqA);
 367                                                 groupIDB = i;
 368                                                 groupID = groupIDB;
 369
 370                                         //      cout << "in bIt: " << groupID << endl;
 371         //                                      break;
 372                                         }
 373
 374                                         if(groupIDA != -1 && groupIDB != -1){//both ifs above have been executed, so we need to decide who to assign them to
 375                                                 if(groupIDA < groupIDB){
 376                                                 //      cout << "A: " << groupIDA << "\t" << groupIDB << endl;
 377                                                         groups[groupIDA].insert(groups[groupIDB].begin(), groups[groupIDB].end()); //merge two groups into groupIDA
 378                                                         groups[groupIDB].clear();
 379                                                         groupID = groupIDA;
 380                                                 }
 381                                                 else{
 382                                                 //      cout << "B: " << groupIDA << "\t" << groupIDB << endl;
 383                                                         groups[groupIDB].insert(groups[groupIDA].begin(), groups[groupIDA].end()); //merge two groups into groupIDB
 384                                                         groups[groupIDA].clear();
 385                                                         groupID = groupIDB;
 386                                                 }
 387                                                 break;
 388                                         }
 389                                 }
 390
 391         //windows is gonna gag on the reuse of outFile, will need to make it local...
 392
 393                                 if(groupIDA == -1 && groupIDB == -1){ //we need a new group
 394                                         set<string> newGroup;
 395                                         newGroup.insert(seqA);
 396                                         newGroup.insert(seqB);
 397                                         groups.push_back(newGroup);
 398
 399                                         string tempOut = seqA + '\t' + seqB + '\t' + toString(dist) + '\n';
 400                                         outputs.push_back(tempOut);
 401                                         numOutputs.push_back(1);
 402                                         wroteOutPut.push_back(false);
 403
 404                                         numGroups++;
 405                                 }
 406                                 else{
 407                                         string fileName = distFile + "." + toString(groupID) + ".temp";
 408
 409                                         //have we reached the max buffer size
 410                                         if (numOutputs[groupID] > 60) { //write out sequence
 411                                                 outFile.open(fileName.c_str(), ios::app);
 412                                                 outFile << outputs[groupID] << seqA << '\t' << seqB << '\t' << dist << endl;
 413                                                 outFile.close();
 414
 415                                                 outputs[groupID] = "";
 416                                                 numOutputs[groupID] = 0;
 417                                                 wroteOutPut[groupID] = true;
 418                                         }else {
 419                                                 outputs[groupID] +=  seqA + '\t' + seqB + '\t' + toString(dist)  + '\n';
 420                                                 numOutputs[groupID]++;
 421                                         }
 422
 423                                         if(groupIDA != -1 && groupIDB != -1){ //merge distance files of two groups you merged above
 424                                                 string row, column, distance;
 425                                                 if(groupIDA<groupIDB){
 426
 427                                                         //merge memory
 428                                                         numOutputs[groupID] += numOutputs[groupIDB];
 429                                                         outputs[groupID] += outputs[groupIDB];
 430
 431                                                         outputs[groupIDB] = "";
 432                                                         numOutputs[groupIDB] = 0;
 433
 434                                                         //if groupB is written to file it is above buffer size so read and write to new merged file
 435                                                         if (wroteOutPut[groupIDB]) {
 436                                                                 string fileName2 = distFile + "." + toString(groupIDB) + ".temp";
 437                                                                 ifstream fileB(fileName2.c_str(), ios::ate);
 438
 439                                                                 outFile.open(fileName.c_str(), ios::app);
 440
 441                                                                 long size;
 442                                                                 char* memblock;
 443
 444                                                                 size = fileB.tellg();
 445
 446                                                                 fileB.seekg (0, ios::beg);
 447
 448                                                                 int numRead = size / 1024;
 449                                                                 int lastRead = size % 1024;
 450
 451                                                                 for (int i = 0; i < numRead; i++) {
 452
 453                                                                         memblock = new char [1024];
 454
 455                                                                         fileB.read (memblock, 1024);
 456
 457                                                                         string temp = memblock;
 458                                                                         outFile << temp.substr(0, 1024);
 459
 460                                                                         delete memblock;
 461                                                                 }
 462
 463                                                                 memblock = new char [lastRead];
 464
 465                                                                 fileB.read (memblock, lastRead);
 466
 467                                                                 //not sure why but it will read more than lastRead char...??
 468                                                                 string temp = memblock;
 469                                                                 outFile << temp.substr(0, lastRead);
 470                                                                 delete memblock;
 471
 472                                                                 fileB.close();
 473                                                                 m->mothurRemove(fileName2);
 474
 475                                                                 //write out the merged memory
 476                                                                 if (numOutputs[groupID] > 60) {
 477                                                                         outFile << outputs[groupID];
 478                                                                         outputs[groupID] = "";
 479                                                                         numOutputs[groupID] = 0;
 480                                                                 }
 481
 482                                                                 outFile.close();
 483
 484                                                                 wroteOutPut[groupID] = true;
 485                                                                 wroteOutPut[groupIDB] = false;
 486                                                         }else{ } //just merge b's memory with a's memory
 487                                                 }
 488                                                 else{
 489                                                         numOutputs[groupID] += numOutputs[groupIDA];
 490                                                         outputs[groupID] += outputs[groupIDA];
 491
 492                                                         outputs[groupIDA] = "";
 493                                                         numOutputs[groupIDA] = 0;
 494
 495                                                         if (wroteOutPut[groupIDA]) {
 496                                                                 string fileName2 = distFile + "." + toString(groupIDA) + ".temp";
 497                                                                 ifstream fileB(fileName2.c_str(), ios::ate);
 498
 499                                                                 outFile.open(fileName.c_str(), ios::app);
 500
 501                                                                 long size;
 502                                                                 char* memblock;
 503
 504                                                                 size = fileB.tellg();
 505
 506                                                                 fileB.seekg (0, ios::beg);
 507
 508                                                                 int numRead = size / 1024;
 509                                                                 int lastRead = size % 1024;
 510
 511                                                                 for (int i = 0; i < numRead; i++) {
 512
 513                                                                         memblock = new char [1024];
 514
 515                                                                         fileB.read (memblock, 1024);
 516                                                                         string temp = memblock;
 517                                                                         outFile << temp.substr(0, 1024);
 518
 519                                                                         delete memblock;
 520                                                                 }
 521
 522                                                                 memblock = new char [lastRead];
 523
 524                                                                 fileB.read (memblock, lastRead);
 525
 526                                                                 //not sure why but it will read more than lastRead char...??
 527                                                                 string temp = memblock;
 528                                                                 outFile << temp.substr(0, lastRead);
 529
 530                                                                 delete memblock;
 531
 532                                                                 fileB.close();
 533                                                                 m->mothurRemove(fileName2);
 534
 535                                                                 //write out the merged memory
 536                                                                 if (numOutputs[groupID] > 60) {
 537                                                                         outFile << outputs[groupID];
 538                                                                         outputs[groupID] = "";
 539                                                                         numOutputs[groupID] = 0;
 540                                                                 }
 541
 542                                                                 outFile.close();
 543
 544                                                                 wroteOutPut[groupID] = true;
 545                                                                 wroteOutPut[groupIDA] = false;
 546                                                         }else { } //just merge memory
 547                                                 }
 548                                         }
 549                                 }
 550                         }
 551                         m->gobble(dFile);
 552                 }
 553                 dFile.close();
 554
 555                 vector<string> tempDistFiles;
 556                 for (int i = 0; i < numGroups; i++) {
 557             string fileName = distFile + "." + toString(i) + ".temp";
 558             tempDistFiles.push_back(fileName);
 559             //remove old names files just in case
 560
 561                         if (numOutputs[i] > 0) {
 562                                 outFile.open(fileName.c_str(), ios::app);
 563                                 outFile << outputs[i];
 564                                 outFile.close();
 565                         }
 566                 }
 567
 568         map<string, int> seqGroup;
 569         for (int i = 0; i < groups.size(); i++) {
 570             for (set<string>::iterator itNames = groups[i].begin(); itNames != groups[i].end();) {
 571                 seqGroup[*itNames] = i;
 572                 groups[i].erase(itNames++);
 573             }
 574         }
 575
 576                 splitNames(seqGroup, numGroups, tempDistFiles);
 577
 578                 return 0;
 579         }
 580         catch(exception& e) {
 581                 m->errorOut(e, "SplitMatrix", "splitDistanceLarge");
 582                 exit(1);
 583         }
 584 }
 585 //********************************************************************************************************************
 586 int SplitMatrix::splitNames(map<string, int>& seqGroup, int numGroups, vector<string>& tempDistFiles){
 587         try {
 588         ofstream outFile;
 589         map<string, int>::iterator it;
 590
 591         string inputFile = namefile;
 592         if (countfile != "") { inputFile = countfile; }
 593
 594         for(int i=0;i<numGroups;i++){  m->mothurRemove((inputFile + "." + toString(i) + ".temp")); }
 595
 596         singleton = inputFile + ".extra.temp";
 597         ofstream remainingNames;
 598         m->openOutputFile(singleton, remainingNames);
 599
 600         bool wroteExtra = false;
 601
 602         ifstream bigNameFile;
 603         m->openInputFile(inputFile, bigNameFile);
 604
 605         //grab header line
 606         string headers = "";
 607         if (countfile != "") { headers = m->getline(bigNameFile); m->gobble(bigNameFile); }
 608
 609         string name, nameList;
 610         while(!bigNameFile.eof()){
 611             bigNameFile >> name >> nameList;
 612             m->getline(bigNameFile); m->gobble(bigNameFile); //extra getline is for rest of countfile line if groups are given.
 613
 614             //did this sequence get assigned a group
 615             it = seqGroup.find(name);
 616
 617             if (it != seqGroup.end()) {
 618                 m->openOutputFileAppend((inputFile + "." + toString(it->second) + ".temp"), outFile);
 619                 outFile << name << '\t' << nameList << endl;
 620                 outFile.close();
 621             }else{
 622                 wroteExtra = true;
 623                 remainingNames << name << '\t' << nameList << endl;
 624             }
 625         }
 626         bigNameFile.close();
 627
 628                 for(int i=0;i<numGroups;i++){
 629                         string tempNameFile = inputFile + "." + toString(i) + ".temp";
 630                         string tempDistFile = tempDistFiles[i];
 631
 632             //if there are valid distances
 633             ifstream fileHandle;
 634             fileHandle.open(tempDistFile.c_str());
 635             if(fileHandle)      {
 636                 m->gobble(fileHandle);
 637                 if (!fileHandle.eof()) {  //check
 638                                 map<string, string> temp;
 639                 if (countfile != "") {
 640                     //add header
 641                     ofstream out;
 642                     string newtempNameFile = tempNameFile + "2";
 643                     m->openOutputFile(newtempNameFile, out);
 644                     out << "Representative_Sequence\ttotal" << endl;
 645                     out.close();
 646                     m->appendFiles(tempNameFile, newtempNameFile);
 647                     m->mothurRemove(tempNameFile);
 648                     m->renameFile(newtempNameFile, tempNameFile);
 649                 }
 650                                 temp[tempDistFile] = tempNameFile;
 651                                 dists.push_back(temp);
 652                         }else{
 653                                 ifstream in;
 654                                 m->openInputFile(tempNameFile, in);
 655
 656                                 while(!in.eof()) {
 657                                         in >> name >> nameList;  m->gobble(in);
 658                                         wroteExtra = true;
 659                                         remainingNames << name << '\t' << nameList << endl;
 660                                 }
 661                                 in.close();
 662                                 m->mothurRemove(tempNameFile);
 663                         }
 664             }
 665             fileHandle.close();
 666                 }
 667
 668                 remainingNames.close();
 669
 670                 if (!wroteExtra) {
 671                         m->mothurRemove(singleton);
 672                         singleton = "none";
 673                 }else if (countfile != "") {
 674             //add header
 675             ofstream out;
 676             string newtempNameFile = singleton + "2";
 677             m->openOutputFile(newtempNameFile, out);
 678             out << "Representative_Sequence\ttotal" << endl;
 679             out.close();
 680             m->appendFiles(singleton, newtempNameFile);
 681             m->mothurRemove(singleton);
 682             m->renameFile(newtempNameFile, singleton);
 683         }
 684
 685                 return 0;
 686         }
 687         catch(exception& e) {
 688                 m->errorOut(e, "SplitMatrix", "splitNames");
 689                 exit(1);
 690         }
 691 }
 692 //********************************************************************************************************************
 693 int SplitMatrix::splitDistanceRAM(){
 694         try {
 695                 vector<set<string> > groups;
 696                 vector<string> outputs;
 697
 698                 int numGroups = 0;
 699
 700                 ifstream dFile;
 701                 m->openInputFile(distFile, dFile);
 702
 703                 while(dFile){
 704                         string seqA, seqB;
 705                         float dist;
 706
 707                         dFile >> seqA >> seqB >> dist;
 708
 709                         if (m->control_pressed) {   dFile.close();  for(int i=0;i<numGroups;i++){       if(groups[i].size() > 0){  m->mothurRemove((distFile + "." + toString(i) + ".temp")); }  } return 0; }
 710
 711                         if(dist < cutoff){
 712                                 //cout << "in cutoff: " << dist << endl;
 713                                 int groupIDA = -1;
 714                                 int groupIDB = -1;
 715                                 int groupID = -1;
 716
 717                                 for(int i=0;i<numGroups;i++){
 718                                         set<string>::iterator aIt = groups[i].find(seqA);
 719                                         set<string>::iterator bIt = groups[i].find(seqB);
 720
 721                                         if(groupIDA == -1 && aIt != groups[i].end()){//seqA is not already assigned to a group and is in group[i], so assign seqB to group[i]
 722                                                 groups[i].insert(seqB);
 723                                                 groupIDA = i;
 724                                                 groupID = groupIDA;
 725
 726                                                 //cout << "in aIt: " << groupID << endl;
 727         //                                      break;
 728                                         }
 729                                         else if(groupIDB == -1 && bIt != groups[i].end()){//seqB is not already assigned to a group and is in group[i], so assign seqA to group[i]
 730                                                 groups[i].insert(seqA);
 731                                                 groupIDB = i;
 732                                                 groupID = groupIDB;
 733
 734                                         //      cout << "in bIt: " << groupID << endl;
 735         //                                      break;
 736                                         }
 737
 738                                         if(groupIDA != -1 && groupIDB != -1){//both ifs above have been executed, so we need to decide who to assign them to
 739                                                 if(groupIDA < groupIDB){
 740                                                 //      cout << "A: " << groupIDA << "\t" << groupIDB << endl;
 741                                                         groups[groupIDA].insert(groups[groupIDB].begin(), groups[groupIDB].end()); //merge two groups into groupIDA
 742                                                         groups[groupIDB].clear();
 743                                                         groupID = groupIDA;
 744                                                 }
 745                                                 else{
 746                                                 //      cout << "B: " << groupIDA << "\t" << groupIDB << endl;
 747                                                         groups[groupIDB].insert(groups[groupIDA].begin(), groups[groupIDA].end()); //merge two groups into groupIDB
 748                                                         groups[groupIDA].clear();
 749                                                         groupID = groupIDB;
 750                                                 }
 751                                                 break;
 752                                         }
 753                                 }
 754
 755         //windows is gonna gag on the reuse of outFile, will need to make it local...
 756
 757                                 if(groupIDA == -1 && groupIDB == -1){ //we need a new group
 758                                         set<string> newGroup;
 759                                         newGroup.insert(seqA);
 760                                         newGroup.insert(seqB);
 761                                         groups.push_back(newGroup);
 762
 763                                         string tempOut = seqA + '\t' + seqB + '\t' + toString(dist) + '\n';
 764                                         outputs.push_back(tempOut);
 765                                         numGroups++;
 766                                 }
 767                                 else{
 768
 769                                         outputs[groupID] +=  seqA + '\t' + seqB + '\t' + toString(dist)  + '\n';
 770
 771                                         if(groupIDA != -1 && groupIDB != -1){ //merge distance files of two groups you merged above
 772                                                 string row, column, distance;
 773                                                 if(groupIDA<groupIDB){
 774                                                         //merge memory
 775                                                         outputs[groupID] += outputs[groupIDB];
 776                                                         outputs[groupIDB] = "";
 777                                                 }else{
 778                                                         outputs[groupID] += outputs[groupIDA];
 779                                                         outputs[groupIDA] = "";
 780                                                 }
 781                                         }
 782                                 }
 783                         }
 784                         m->gobble(dFile);
 785                 }
 786                 dFile.close();
 787
 788         vector<string> tempDistFiles;
 789                 for (int i = 0; i < numGroups; i++) {
 790             string fileName = distFile + "." + toString(i) + ".temp";
 791             tempDistFiles.push_back(fileName);
 792                         if (outputs[i] != "") {
 793                                 ofstream outFile;
 794                                 outFile.open(fileName.c_str(), ios::ate);
 795                                 outFile << outputs[i];
 796                                 outFile.close();
 797                         }
 798                 }
 799
 800         map<string, int> seqGroup;
 801         for (int i = 0; i < groups.size(); i++) {
 802             for (set<string>::iterator itNames = groups[i].begin(); itNames != groups[i].end();) {
 803                 seqGroup[*itNames] = i;
 804                 groups[i].erase(itNames++);
 805             }
 806         }
 807
 808                 splitNames(seqGroup, numGroups, tempDistFiles);
 809
 810                 return 0;
 811         }
 812         catch(exception& e) {
 813                 m->errorOut(e, "SplitMatrix", "splitDistanceRAM");
 814                 exit(1);
 815         }
 816 }
 817 //********************************************************************************************************************
 818 //sorts biggest to smallest
 819 inline bool compareFileSizes(map<string, string> left, map<string, string> right){
 820
 821         FILE * pFile;
 822         long leftsize = 0;
 823
 824         //get num bytes in file
 825         string filename = left.begin()->first;
 826         pFile = fopen (filename.c_str(),"rb");
 827         string error = "Error opening " + filename;
 828         if (pFile==NULL) perror (error.c_str());
 829         else{
 830                 fseek (pFile, 0, SEEK_END);
 831                 leftsize=ftell (pFile);
 832                 fclose (pFile);
 833         }
 834
 835         FILE * pFile2;
 836         long rightsize = 0;
 837
 838         //get num bytes in file
 839         filename = right.begin()->first;
 840         pFile2 = fopen (filename.c_str(),"rb");
 841         error = "Error opening " + filename;
 842         if (pFile2==NULL) perror (error.c_str());
 843         else{
 844                 fseek (pFile2, 0, SEEK_END);
 845                 rightsize=ftell (pFile2);
 846                 fclose (pFile2);
 847         }
 848
 849         return (leftsize > rightsize);
 850 }
 851 /***********************************************************************/
 852 //returns map of distance files -> namefile sorted by distance file size
 853 vector< map< string, string> > SplitMatrix::getDistanceFiles(){
 854         try {
 855
 856                 sort(dists.begin(), dists.end(), compareFileSizes);
 857
 858                 return dists;
 859         }
 860         catch(exception& e) {
 861                 m->errorOut(e, "SplitMatrix", "getDistanceFiles");
 862                 exit(1);
 863         }
 864 }
 865 /***********************************************************************/
 866 SplitMatrix::~SplitMatrix(){}
 867 /***********************************************************************/
 868