reversecommand.cpp

   1 /*
   2  *  reversecommand.cpp
   3  *  Mothur
   4  *
   5  *  Created by Pat Schloss on 6/6/09.
   6  *  Copyright 2009 Patrick D. Schloss. All rights reserved.
   7  *
   8  */
   9
  10 #include "reversecommand.h"
  11 #include "sequence.hpp"
  12 #include "qualityscores.h"
  13
  14 //**********************************************************************************************************************
  15 vector<string> ReverseSeqsCommand::setParameters(){
  16         try {
  17                 CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "fastaQual", "none",false,false); parameters.push_back(pfasta);
  18                 CommandParameter pqfile("qfile", "InputTypes", "", "", "none", "fastaQual", "none",false,false); parameters.push_back(pqfile);
  19                 CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
  20                 CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir);
  21
  22                 vector<string> myArray;
  23                 for (int i = 0; i < parameters.size(); i++) {   myArray.push_back(parameters[i].name);          }
  24                 return myArray;
  25         }
  26         catch(exception& e) {
  27                 m->errorOut(e, "ReverseSeqsCommand", "setParameters");
  28                 exit(1);
  29         }
  30 }
  31 //**********************************************************************************************************************
  32 string ReverseSeqsCommand::getHelpString(){
  33         try {
  34                 string helpString = "";
  35                 helpString += "The reverse.seqs command reads a fastafile and outputs a fasta file containing the reverse compliment.\n";
  36                 helpString += "The reverse.seqs command parameters fasta or qfile are required.\n";
  37                 helpString += "The reverse.seqs command should be in the following format: \n";
  38                 helpString += "reverse.seqs(fasta=yourFastaFile) \n";
  39                 return helpString;
  40         }
  41         catch(exception& e) {
  42                 m->errorOut(e, "ReverseSeqsCommand", "getHelpString");
  43                 exit(1);
  44         }
  45 }
  46
  47 //**********************************************************************************************************************
  48 ReverseSeqsCommand::ReverseSeqsCommand(){
  49         try {
  50                 abort = true; calledHelp = true;
  51                 setParameters();
  52                 vector<string> tempOutNames;
  53                 outputTypes["fasta"] = tempOutNames;
  54                 outputTypes["qfile"] = tempOutNames;
  55         }
  56         catch(exception& e) {
  57                 m->errorOut(e, "ReverseSeqsCommand", "ReverseSeqsCommand");
  58                 exit(1);
  59         }
  60 }
  61 //***************************************************************************************************************
  62
  63 ReverseSeqsCommand::ReverseSeqsCommand(string option)  {
  64         try {
  65                 abort = false; calledHelp = false;
  66
  67                 //allow user to run help
  68                 if(option == "help") { help(); abort = true; calledHelp = true; }
  69
  70                 else {
  71                         vector<string> myArray = setParameters();
  72
  73                         OptionParser parser(option);
  74                         map<string,string> parameters = parser.getParameters();
  75
  76                         ValidParameters validParameter;
  77                         map<string,string>::iterator it;
  78
  79                         //check to make sure all parameters are valid for command
  80                         for (it = parameters.begin(); it != parameters.end(); it++) {
  81                                 if (validParameter.isValidParameter(it->first, myArray, it->second) != true) {  abort = true;  }
  82                         }
  83
  84                         //initialize outputTypes
  85                         vector<string> tempOutNames;
  86                         outputTypes["fasta"] = tempOutNames;
  87                         outputTypes["qfile"] = tempOutNames;
  88
  89                         //if the user changes the input directory command factory will send this info to us in the output parameter
  90                         string inputDir = validParameter.validFile(parameters, "inputdir", false);
  91                         if (inputDir == "not found"){   inputDir = "";          }
  92                         else {
  93                                 string path;
  94                                 it = parameters.find("fasta");
  95                                 //user has given a template file
  96                                 if(it != parameters.end()){
  97                                         path = m->hasPath(it->second);
  98                                         //if the user has not given a path then, add inputdir. else leave path alone.
  99                                         if (path == "") {       parameters["fasta"] = inputDir + it->second;            }
 100                                 }
 101
 102                                 it = parameters.find("qfile");
 103                                 //user has given a template file
 104                                 if(it != parameters.end()){
 105                                         path = m->hasPath(it->second);
 106                                         //if the user has not given a path then, add inputdir. else leave path alone.
 107                                         if (path == "") {       parameters["qfile"] = inputDir + it->second;            }
 108                                 }
 109                         }
 110
 111                         //check for required parameters
 112                         fastaFileName = validParameter.validFile(parameters, "fasta", true);
 113                         if (fastaFileName == "not open") { abort = true; }
 114                         else if (fastaFileName == "not found") { fastaFileName = "";}// m->mothurOut("fasta is a required parameter for the reverse.seqs command."); m->mothurOutEndLine(); abort = true;  }
 115
 116                         qualFileName = validParameter.validFile(parameters, "qfile", true);
 117                         if (qualFileName == "not open") { abort = true; }
 118                         else if (qualFileName == "not found") { qualFileName = ""; }//m->mothurOut("fasta is a required parameter for the reverse.seqs command."); m->mothurOutEndLine(); abort = true;  }
 119
 120                         if((fastaFileName == "") && (qualFileName == "")){
 121                                 fastaFileName = m->getFastaFile();
 122                                 if (fastaFileName != "") {  m->mothurOut("Using " + fastaFileName + " as input file for the fasta parameter."); m->mothurOutEndLine(); }
 123                                 else {
 124                                         qualFileName = m->getQualFile();
 125                                         if (qualFileName != "") {  m->mothurOut("Using " + qualFileName + " as input file for the qfile parameter."); m->mothurOutEndLine(); }
 126                                         else {
 127                                                 m->mothurOut("You have no current files for fasta or qfile, and fasta or qfile is a required parameter for the reverse.seqs command."); m->mothurOutEndLine();
 128                                                 abort = true;
 129                                         }
 130                                 }
 131                         }
 132
 133                         //if the user changes the output directory command factory will send this info to us in the output parameter
 134                         outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){
 135                                 outputDir = "";
 136                         }
 137
 138                 }
 139         }
 140         catch(exception& e) {
 141                 m->errorOut(e, "ReverseSeqsCommand", "ReverseSeqsCommand");
 142                 exit(1);
 143         }
 144 }
 145 //***************************************************************************************************************
 146
 147
 148 int ReverseSeqsCommand::execute(){
 149         try{
 150
 151                 if (abort == true) { if (calledHelp) { return 0; }  return 2;   }
 152
 153                 string fastaReverseFileName;
 154
 155                 if(fastaFileName != ""){
 156                         ifstream inFASTA;
 157                         m->openInputFile(fastaFileName, inFASTA);
 158
 159                         ofstream outFASTA;
 160                         string tempOutputDir = outputDir;
 161                         if (outputDir == "") { tempOutputDir += m->hasPath(fastaFileName); } //if user entered a file with a path then preserve it
 162                         fastaReverseFileName = tempOutputDir + m->getRootName(m->getSimpleName(fastaFileName)) + "rc" + m->getExtension(fastaFileName);
 163                         m->openOutputFile(fastaReverseFileName, outFASTA);
 164
 165                         while(!inFASTA.eof()){
 166                                 if (m->control_pressed) {  inFASTA.close();  outFASTA.close(); remove(fastaReverseFileName.c_str()); return 0; }
 167
 168                                 Sequence currSeq(inFASTA);  m->gobble(inFASTA);
 169                                 if (currSeq.getName() != "") {
 170                                         currSeq.reverseComplement();
 171                                         currSeq.printSequence(outFASTA);
 172                                 }
 173                         }
 174                         inFASTA.close();
 175                         outFASTA.close();
 176                         outputNames.push_back(fastaReverseFileName); outputTypes["fasta"].push_back(fastaReverseFileName);
 177                 }
 178
 179                 string qualReverseFileName;
 180
 181                 if(qualFileName != ""){
 182                         QualityScores currQual;
 183
 184                         ifstream inQual;
 185                         m->openInputFile(qualFileName, inQual);
 186
 187                         ofstream outQual;
 188                         string tempOutputDir = outputDir;
 189                         if (outputDir == "") { tempOutputDir += m->hasPath(qualFileName); } //if user entered a file with a path then preserve it
 190                         string qualReverseFileName = tempOutputDir + m->getRootName(m->getSimpleName(qualFileName)) + "rc" + m->getExtension(qualFileName);
 191                         m->openOutputFile(qualReverseFileName, outQual);
 192
 193                         while(!inQual.eof()){
 194                                 if (m->control_pressed) {  inQual.close();  outQual.close(); remove(qualReverseFileName.c_str()); return 0; }
 195                                 currQual = QualityScores(inQual);  m->gobble(inQual);
 196                                 currQual.flipQScores();
 197                                 currQual.printQScores(outQual);
 198                         }
 199                         inQual.close();
 200                         outQual.close();
 201                         outputNames.push_back(qualReverseFileName); outputTypes["qfile"].push_back(qualReverseFileName);
 202                 }
 203
 204                 if (m->control_pressed) {  remove(qualReverseFileName.c_str()); remove(fastaReverseFileName.c_str()); return 0; }
 205
 206                 //set fasta file as new current fastafile
 207                 string current = "";
 208                 itTypes = outputTypes.find("fasta");
 209                 if (itTypes != outputTypes.end()) {
 210                         if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setFastaFile(current); }
 211                 }
 212
 213                 itTypes = outputTypes.find("qfile");
 214                 if (itTypes != outputTypes.end()) {
 215                         if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setQualFile(current); }
 216                 }
 217
 218
 219                 m->mothurOutEndLine();
 220                 m->mothurOut("Output File Name: "); m->mothurOutEndLine();
 221                 for(int i=0;i<outputNames.size();i++){
 222                         m->mothurOut(outputNames[i]);
 223                         m->mothurOutEndLine();
 224                 }
 225
 226
 227
 228                 return 0;
 229         }
 230         catch(exception& e) {
 231                 m->errorOut(e, "ReverseSeqsCommand", "execute");
 232                 exit(1);
 233         }
 234 }
 235
 236 //***************************************************************************************************************