pipelinepdscommand.cpp

   1 /*
   2  *  pipelinepdscommand.cpp
   3  *  Mothur
   4  *
   5  *  Created by westcott on 10/5/10.
   6  *  Copyright 2010 Schloss Lab. All rights reserved.
   7  *
   8  */
   9
  10 #include "pipelinepdscommand.h"
  11 #include "sffinfocommand.h"
  12 #include "commandoptionparser.hpp"
  13
  14 //**********************************************************************************************************************
  15 vector<string> PipelineCommand::getValidParameters(){
  16         try {
  17                 string Array[] =  {"sff","oligos","align","chimera","classify","taxonomy","pipeline","processors","outputdir","inputdir"};
  18                 vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
  19                 return myArray;
  20         }
  21         catch(exception& e) {
  22                 m->errorOut(e, "PipelineCommand", "getValidParameters");
  23                 exit(1);
  24         }
  25 }
  26 //**********************************************************************************************************************
  27 vector<string> PipelineCommand::getRequiredParameters(){
  28         try {
  29                 vector<string> myArray;
  30                 return myArray;
  31         }
  32         catch(exception& e) {
  33                 m->errorOut(e, "PipelineCommand", "getRequiredParameters");
  34                 exit(1);
  35         }
  36 }
  37 //**********************************************************************************************************************
  38 vector<string> PipelineCommand::getRequiredFiles(){
  39         try {
  40                 vector<string> myArray;
  41                 return myArray;
  42         }
  43         catch(exception& e) {
  44                 m->errorOut(e, "PipelineCommand", "getRequiredFiles");
  45                 exit(1);
  46         }
  47 }
  48 //**********************************************************************************************************************
  49 PipelineCommand::PipelineCommand(string option) {
  50         try {
  51                 cFactory = CommandFactory::getInstance();
  52                 abort = false;
  53
  54                 //allow user to run help
  55                 if(option == "help") { help(); abort = true; }
  56
  57                 else {
  58
  59                         //valid paramters for this command
  60                         string AlignArray[] =  {"sff","oligos","align","chimera","classify","taxonomy","pipeline","processors","outputdir","inputdir"};
  61                         vector<string> myArray (AlignArray, AlignArray+(sizeof(AlignArray)/sizeof(string)));
  62
  63                         OptionParser parser(option);
  64                         map<string, string> parameters = parser.getParameters();
  65
  66                         ValidParameters validParameter;
  67                         map<string, string>::iterator it;
  68
  69                         //check to make sure all parameters are valid for command
  70                         for (it = parameters.begin(); it != parameters.end(); it++) {
  71                                 if (validParameter.isValidParameter(it->first, myArray, it->second) != true) {  abort = true;  }
  72                         }
  73
  74                         //if the user changes the input directory command factory will send this info to us in the output parameter
  75                         string inputDir = validParameter.validFile(parameters, "inputdir", false);
  76                         if (inputDir == "not found"){   inputDir = "";          }
  77                         else {
  78                                 string path;
  79                                 it = parameters.find("sff");
  80                                 //user has given a template file
  81                                 if(it != parameters.end()){
  82                                         path = m->hasPath(it->second);
  83                                         //if the user has not given a path then, add inputdir. else leave path alone.
  84                                         if (path == "") {       parameters["sff"] = inputDir + it->second;              }
  85                                 }
  86
  87                                 it = parameters.find("oligos");
  88                                 //user has given a template file
  89                                 if(it != parameters.end()){
  90                                         path = m->hasPath(it->second);
  91                                         //if the user has not given a path then, add inputdir. else leave path alone.
  92                                         if (path == "") {       parameters["oligos"] = inputDir + it->second;           }
  93                                 }
  94
  95                                 it = parameters.find("align");
  96                                 //user has given a template file
  97                                 if(it != parameters.end()){
  98                                         path = m->hasPath(it->second);
  99                                         //if the user has not given a path then, add inputdir. else leave path alone.
 100                                         if (path == "") {       parameters["align"] = inputDir + it->second;            }
 101                                 }
 102
 103                                 it = parameters.find("chimera");
 104                                 //user has given a template file
 105                                 if(it != parameters.end()){
 106                                         path = m->hasPath(it->second);
 107                                         //if the user has not given a path then, add inputdir. else leave path alone.
 108                                         if (path == "") {       parameters["chimera"] = inputDir + it->second;          }
 109                                 }
 110
 111                                 it = parameters.find("classify");
 112                                 //user has given a template file
 113                                 if(it != parameters.end()){
 114                                         path = m->hasPath(it->second);
 115                                         //if the user has not given a path then, add inputdir. else leave path alone.
 116                                         if (path == "") {       parameters["classify"] = inputDir + it->second;         }
 117                                 }
 118
 119                                 it = parameters.find("taxonomy");
 120                                 //user has given a template file
 121                                 if(it != parameters.end()){
 122                                         path = m->hasPath(it->second);
 123                                         //if the user has not given a path then, add inputdir. else leave path alone.
 124                                         if (path == "") {       parameters["taxonomy"] = inputDir + it->second;         }
 125                                 }
 126
 127                                 it = parameters.find("pipeline");
 128                                 //user has given a template file
 129                                 if(it != parameters.end()){
 130                                         path = m->hasPath(it->second);
 131                                         //if the user has not given a path then, add inputdir. else leave path alone.
 132                                         if (path == "") {       parameters["pipeline"] = inputDir + it->second;         }
 133                                 }
 134                         }
 135
 136                         outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){  outputDir = ""; }
 137
 138                         pipeFilename = validParameter.validFile(parameters, "pipeline", true);
 139                         if (pipeFilename == "not found") { pipeFilename = "";  }
 140                         else if (pipeFilename == "not open") { pipeFilename = ""; abort = true; }
 141
 142                         string temp = validParameter.validFile(parameters, "processors", false);        if (temp == "not found"){       temp = "1";                             }
 143                         convert(temp, processors);
 144
 145                         if (pipeFilename != "") {
 146                                 abort = readUsersPipeline();
 147                         }else{
 148                                 sffFile = validParameter.validFile(parameters, "sff", true);
 149                                 if (sffFile == "not found") { m->mothurOut("sff is a required parameter for the pipeline command."); m->mothurOutEndLine(); abort = true;  }
 150                                 else if (sffFile == "not open") { sffFile = ""; abort = true; }
 151
 152                                 oligosFile = validParameter.validFile(parameters, "oligos", true);
 153                                 if (oligosFile == "not found") { m->mothurOut("oligos is a required parameter for the pipeline command."); m->mothurOutEndLine(); abort = true;  }
 154                                 else if (oligosFile == "not open") { oligosFile = ""; abort = true; }
 155
 156                                 alignFile = validParameter.validFile(parameters, "align", true);
 157                                 if (alignFile == "not found") { m->mothurOut("align is a required parameter for the pipeline command. Please provide the template to align with."); m->mothurOutEndLine(); abort = true;  }
 158                                 else if (alignFile == "not open") { alignFile = ""; abort = true; }
 159
 160                                 chimeraFile = validParameter.validFile(parameters, "chimera", true);
 161                                 if (chimeraFile == "not found") { m->mothurOut("chimera is a required parameter for the pipeline command. Please provide the template to check for chimeras with."); m->mothurOutEndLine(); abort = true;  }
 162                                 else if (chimeraFile == "not open") { chimeraFile = ""; abort = true; }
 163
 164                                 classifyFile = validParameter.validFile(parameters, "classify", true);
 165                                 if (classifyFile == "not found") { m->mothurOut("classify is a required parameter for the pipeline command. Please provide the template to use with the classifier."); m->mothurOutEndLine(); abort = true;  }
 166                                 else if (classifyFile == "not open") { classifyFile = ""; abort = true; }
 167
 168                                 taxonomyFile = validParameter.validFile(parameters, "taxonomy", true);
 169                                 if (taxonomyFile == "not found") { m->mothurOut("taxonomy is a required parameter for the pipeline command."); m->mothurOutEndLine(); abort = true;  }
 170                                 else if (taxonomyFile == "not open") { taxonomyFile = ""; abort = true; }
 171                         }
 172                 }
 173
 174         }
 175         catch(exception& e) {
 176                 m->errorOut(e, "PipelineCommand", "PipelineCommand");
 177                 exit(1);
 178         }
 179 }
 180 //**********************************************************************************************************************
 181
 182 void PipelineCommand::help(){
 183         try {
 184                  m->mothurOut("The pipeline command is designed to guide you through your analysis using mothur.\n");
 185                  m->mothurOut("The pipeline command parameters are pipeline, sff, oligos, align, chimera, classify, taxonomy and processors.\n");
 186                  m->mothurOut("The sff parameter allows you to enter your sff file. It is required.\n");
 187                  m->mothurOut("The oligos parameter allows you to enter your oligos file. It is required.\n");
 188                  m->mothurOut("The align parameter allows you to enter a template to use with the aligner. It is required.\n");
 189                  m->mothurOut("The chimera parameter allows you to enter a template to use for chimera detection. It is required.\n");
 190                  m->mothurOut("The classify parameter allows you to enter a template to use for classification. It is required.\n");
 191                  m->mothurOut("The taxonomy parameter allows you to enter a taxonomy file for the classify template to use for classification. It is required.\n");
 192                  m->mothurOut("The processors parameter allows you to specify the number of processors to use. The default is 1.\n");
 193                  m->mothurOut("The pipeline parameter allows you to enter your own pipeline file. This file should look like a mothur batchfile, but where you would be using a mothur generated file, you can use mothurmade instead.\n");
 194                  m->mothurOut("First column contains the command name, and the second column contains the parameter options or 'defaults', meaning use defaults. You may leave out file options.\n");
 195                  m->mothurOut("Example: trim.seqs(processors=8, allfiles=T, maxambig=0, maxhomop=8, flip=T, bdiffs=1, pdiffs=2, qwindowaverage=35, qwindowsize=50, fasta=may1.v13.fasta, oligos=may1.v13.oligos, qfile=may1.v13.qual)\n");
 196                  m->mothurOut("then, you could enter unique.seqs(fasta=mothurmade), and mothur would use the .trim.fasta file from the trim.seqs command. \n");
 197                  m->mothurOut("then you could enter align.seqs(candidate=mothurmade, template=silva.v13.align, processors=8). , and mothur would use the .trim.unique.fasta file from the unique.seqs command. \n");
 198                  m->mothurOut("If no pipeline file is given then mothur will use Pat's pipeline. \n\n");
 199                  m->mothurOut("Here is a list of the commands used in Pat's pipeline.\n");
 200                  m->mothurOut("All paralellized commands will use the processors you entered.\n");
 201                  m->mothurOut("The sffinfo command takes your sff file and extracts the fasta and quality files.\n");
 202                  m->mothurOut("The trim.seqs command uses your oligos file and the quality and fasta files generated by sffinfo.\n");
 203                  m->mothurOut("The trim.seqs command sets the following parameters: allfiles=T, maxambig=0, maxhomop=8, flip=T, bdiffs=1, pdiffs=2, qwindowaverage=35, qwindowsize=50.\n");
 204                  m->mothurOut("The unique.seqs command uses the trimmed fasta file and removes redundant sequences, don't worry the names file generated by unique.seqs will be used in the pipeline to make sure they are included.\n");
 205                  m->mothurOut("The align.seqs command aligns the unique sequences using the aligners default options. \n");
 206                  m->mothurOut("The screen.seqs command screens the sequences using optimize=end-minlength. \n");
 207                  m->mothurOut("The pipeline uses chimera.slayer to detect chimeras using the default options. \n");
 208                  m->mothurOut("The pipeline removes all sequences determined to be chimeric by chimera.slayer. \n");
 209                  m->mothurOut("The filter.seqs command filters the sequences using vertical=T, trump=. \n");
 210                  m->mothurOut("The unique.seqs command uses the filtered fasta file and name file to remove sequences that have become redundant after filtering.\n");
 211                  m->mothurOut("The pre.cluster command clusters sequences that have no more than 2 differences.\n");
 212                  m->mothurOut("The dist.seqs command is used to generate a column and phylip formatted distance matrix using cutoff=0.20 for column.\n");
 213                  m->mothurOut("The pipeline uses cluster with method=average, hard=T. \n");
 214                  m->mothurOut("The classify.seqs command is used to classify the sequences using the bayesian method with a cutoff of 80.\n");
 215                  m->mothurOut("The phylotype command is used to cluster the sequences based on their classification.\n");
 216                  m->mothurOut("The clearcut command is used to generate a tree using neighbor=T. \n");
 217                  m->mothurOut("The summary.single and summary.shared commands are run on the otu files from cluster and phylotype commands. \n");
 218                  m->mothurOut("The summary.shared command uses calc=sharednseqs-sharedsobs-sharedchao-sharedace-anderberg-jclass-jest-kulczynski-kulczynskicody-lennon-ochiai-sorclass-sorest-whittaker-braycurtis-jabund-morisitahorn-sorabund-thetan-thetayc. \n");
 219                  m->mothurOut("The summary.single command uses calc=nseqs-sobs-coverage-bergerparker-chao-ace-jack-bootstrap-boneh-efron-shen-solow-shannon-npshannon-invsimpson-qstat-simpsoneven-shannoneven-heip-smithwilson. \n");
 220                  m->mothurOut("The classify.otu command is used to get the concensus taxonomy for otu files from cluster and phylotype commands. \n");
 221                  m->mothurOut("The phylo.diversity command run on the tree generated by clearcut with rarefy=T, iters=100. \n");
 222                  m->mothurOut("The unifrac commands are also run on the tree generated by clearcut with random=F, distance=T. \n");
 223                  m->mothurOut("\n\n");
 224         }
 225         catch(exception& e) {
 226                 m->errorOut(e, "PipelineCommand", "help");
 227                 exit(1);
 228         }
 229 }
 230
 231 //**********************************************************************************************************************
 232
 233 PipelineCommand::~PipelineCommand(){}
 234
 235 //**********************************************************************************************************************
 236
 237 int PipelineCommand::execute(){
 238         try {
 239                 if (abort == true) { return 0; }
 240
 241                 int start = time(NULL);
 242
 243                 if (pipeFilename == "") {
 244                         createPatsPipeline();
 245
 246                         //run Pats pipeline
 247                         for (int i = 0; i < commands.size(); i++) {
 248                                 m->mothurOutEndLine(); m->mothurOut("mothur > " + commands[i]); m->mothurOutEndLine();
 249
 250                                 if (m->control_pressed) { return 0; }
 251
 252                                 CommandOptionParser parser(commands[i]);
 253                                 string commandName = parser.getCommandString();
 254                                 string options = parser.getOptionString();
 255
 256                                 #ifdef USE_MPI
 257                                         int pid;
 258                                         MPI_Comm_rank(MPI_COMM_WORLD, &pid);
 259
 260                                         if ((cFactory->MPIEnabled(commandName)) || (pid == 0)) {
 261                                 #endif
 262
 263                                 //executes valid command
 264                                 Command* command = cFactory->getCommand(commandName, options, "pipe");
 265                                 command->execute();
 266
 267                                 //add output files to list
 268                                 map<string, vector<string> > thisCommandsFile = command->getOutputFiles();
 269                                 map<string, vector<string> >::iterator itMade;
 270                                 for (itMade = thisCommandsFile.begin(); itMade != thisCommandsFile.end(); itMade++) {
 271                                         vector<string> temp = itMade->second;
 272                                         for (int j = 0; j < temp.size(); j++) { outputNames.push_back(temp[j]); }
 273                                 }
 274
 275                                 #ifdef USE_MPI
 276                                         }
 277                                 #endif
 278                         }
 279
 280                 }else {  runUsersPipeline(); }
 281
 282                 if (m->control_pressed) { return 0; }
 283
 284                 m->mothurOut("It took " + toString(time(NULL) - start) + " secs to run the pipeline analysis."); m->mothurOutEndLine(); m->mothurOutEndLine();
 285
 286                 m->mothurOutEndLine();
 287                 m->mothurOut("Output File Names: "); m->mothurOutEndLine();
 288                 for (int i = 0; i < outputNames.size(); i++) {  m->mothurOut(outputNames[i]); m->mothurOutEndLine();    }
 289                 m->mothurOutEndLine();
 290
 291                 return 0;
 292         }
 293         catch(exception& e) {
 294                 m->errorOut(e, "PipelineCommand", "execute");
 295                 exit(1);
 296         }
 297 }
 298 //**********************************************************************************************************************
 299
 300 bool PipelineCommand::readUsersPipeline(){
 301         try {
 302
 303                 ifstream in;
 304                 m->openInputFile(pipeFilename, in);
 305
 306                 string nextCommand = "";
 307
 308                 map<string, vector<string> > mothurMadeFiles;
 309
 310                 while(!in.eof()) {
 311                         nextCommand = m->getline(in); m->gobble(in);
 312
 313                         if (nextCommand[0] != '#') {
 314                                 bool error = false;
 315
 316                                 string commandName, options;
 317                                 error = parseCommand(nextCommand, commandName, options);
 318
 319                                 if (error) { in.close(); return error; }
 320                                 if (commandName == "pipeline.pds") { m->mothurOut("Cannot run the pipeline.pds command from inside the pipeline.pds command."); m->mothurOutEndLine(); in.close(); return true; }
 321
 322                                 error = checkForValidAndRequiredParameters(commandName, options, mothurMadeFiles);
 323
 324                                 if (error) { in.close(); return error; }
 325                         }
 326                 }
 327
 328                 in.close();
 329
 330                 return false;
 331         }
 332         catch(exception& e) {
 333                 m->errorOut(e, "PipelineCommand", "readUsersPipeline");
 334                 exit(1);
 335         }
 336 }
 337 //**********************************************************************************************************************
 338
 339 bool PipelineCommand::parseCommand(string nextCommand, string& name, string& options){
 340         try {
 341                 CommandOptionParser parser(nextCommand);
 342                 name = parser.getCommandString();
 343                 options = parser.getOptionString();
 344
 345                 if (name == "") { return true; } //name == "" if () are not right
 346
 347                 return false;
 348         }
 349         catch(exception& e) {
 350                 m->errorOut(e, "PipelineCommand", "parseCommand");
 351                 exit(1);
 352         }
 353 }
 354 //**********************************************************************************************************************
 355
 356 bool PipelineCommand::checkForValidAndRequiredParameters(string name, string options, map<string, vector<string> >& mothurMadeFiles){
 357         try {
 358
 359                 //get shell of the command so we can check to make sure its valid without running it
 360                 Command* command = cFactory->getCommand(name);
 361
 362                 //check to make sure all parameters are valid for command
 363                 vector<string> validParameters = command->getValidParameters();
 364
 365                 OptionParser parser(options);
 366                 map<string, string> parameters = parser.getParameters();
 367
 368                 ValidParameters validParameter;
 369                 map<string, string>::iterator it;
 370                 map<string, vector<string> >::iterator itMade;
 371
 372                 for (it = parameters.begin(); it != parameters.end(); it++) {
 373                         if (validParameter.isValidParameter(it->first, validParameters, it->second) != true) {  return true;  } // not valid
 374                         if (it->second == "mothurmade") {
 375                                 itMade = mothurMadeFiles.find(it->first);
 376
 377                                 if (itMade == mothurMadeFiles.end()) {
 378                                         m->mothurOut("You have the " + it->first + " listed as a mothurmade file for the " + name + " command, but it seems mothur will not make that file in your current pipeline, please correct."); m->mothurOutEndLine();
 379                                         return true;
 380                                 }
 381                         }
 382                 }
 383
 384                 //is the command missing any required
 385                 vector<string> requiredParameters = command->getRequiredParameters();
 386
 387                 //check for or
 388                 bool hasOr = false;
 389                 int numFound = 0;
 390                 if (requiredParameters.size() > 2) {
 391                         if (requiredParameters[(requiredParameters.size()-1)] == "or") { hasOr = true; }
 392                 }
 393
 394                 for (int i = 0; i < requiredParameters.size(); i++) {
 395                         it = parameters.find(requiredParameters[i]);
 396
 397                         if (it != parameters.end()) { numFound++; }
 398                         else {
 399                                 if (!hasOr) { m->mothurOut(name + " requires the " + requiredParameters[i] + " parameter, please correct."); m->mothurOutEndLine(); }
 400                         }
 401                 }
 402
 403                 // if all are needed and not all are found
 404                 if ((!hasOr) && (numFound != requiredParameters.size())) { return true; }
 405                 //if one is needed and none are found
 406                 else if ((hasOr) && (numFound == 0)) { return true; }
 407
 408                 //update MothurMade
 409                 map<string, vector<string> > thisCommandsFile = command->getOutputFiles();
 410                 for (itMade = thisCommandsFile.begin(); itMade != thisCommandsFile.end(); itMade++) {
 411                         mothurMadeFiles[itMade->first] = itMade->second; //adds any new types
 412                 }
 413
 414                 return false;
 415         }
 416         catch(exception& e) {
 417                 m->errorOut(e, "PipelineCommand", "checkForValidAndRequiredParameters");
 418                 exit(1);
 419         }
 420 }
 421 //**********************************************************************************************************************
 422 int PipelineCommand::runUsersPipeline(){
 423         try {
 424                 ifstream in;
 425                 m->openInputFile(pipeFilename, in);
 426
 427                 string nextCommand = "";
 428
 429                 map<string, vector<string> > mothurMadeFiles;
 430
 431                 while(!in.eof()) {
 432                         nextCommand = m->getline(in);  m->gobble(in);
 433
 434                         if (nextCommand[0] != '#') {
 435                                 CommandOptionParser parser(nextCommand);
 436                                 string commandName = parser.getCommandString();
 437                                 string options = parser.getOptionString();
 438
 439                                 if (options != "") {
 440                                         bool error = fillInMothurMade(options, mothurMadeFiles);
 441                                         if (error) { in.close(); return 0; }
 442                                 }
 443
 444                                 m->mothurOutEndLine(); m->mothurOut("mothur > " + commandName + "(" + options + ")"); m->mothurOutEndLine();
 445
 446                                 if (m->control_pressed) { return 0; }
 447
 448                                 #ifdef USE_MPI
 449                                         int pid;
 450                                         MPI_Comm_rank(MPI_COMM_WORLD, &pid);
 451
 452                                         if ((cFactory->MPIEnabled(commandName)) || (pid == 0)) {
 453                                 #endif
 454
 455                                 //executes valid command
 456                                 Command* command = cFactory->getCommand(commandName, options, "pipe");
 457                                 command->execute();
 458
 459                                 //add output files to list
 460                                 map<string, vector<string> > thisCommandsFile = command->getOutputFiles();
 461                                 map<string, vector<string> >::iterator itMade;
 462                                 map<string, vector<string> >::iterator it;
 463                                 for (itMade = thisCommandsFile.begin(); itMade != thisCommandsFile.end(); itMade++) {
 464
 465                                         vector<string> temp = itMade->second;
 466                                         for (int k = 0; k < temp.size(); k++) { outputNames.push_back(temp[k]); }  //
 467
 468                                         //update Mothur Made for each file
 469                                         it = mothurMadeFiles.find(itMade->first);
 470
 471                                         if (it == mothurMadeFiles.end()) { //new type
 472
 473                                                 mothurMadeFiles[itMade->first] = temp;
 474
 475                                         }else{ //update existing type
 476                                                 vector<string> oldFileNames = it->second;
 477                                                 //look at new files, see if an old version of the file exists, if so update, else just add.
 478                                                 //for example you may have abrecovery.fasta and amazon.fasta as old files and you created a new amazon.trim.fasta.
 479
 480                                                 for (int k = 0; k < temp.size(); k++) {
 481
 482                                                         //get base name
 483                                                         string root = m->getSimpleName(temp[k]);
 484                                                         string individual = "";
 485                                                         for(int i=0;i<root.length();i++){
 486                                                                 if(root[i] == '.'){
 487                                                                         root = individual;
 488                                                                         break;
 489                                                                 }else{
 490                                                                         individual += root[i];
 491                                                                 }
 492                                                         }
 493
 494                                                         //look for that base name in oldfiles
 495                                                         int spot = -1;
 496                                                         for (int l = 0; l < oldFileNames.size(); l++) {
 497                                                                 int pos = oldFileNames[l].find(root);
 498                                                                 if (pos != string::npos) {
 499                                                                         spot = l;
 500                                                                         break;
 501                                                                 }
 502                                                         }
 503
 504                                                         //if you found it update it, else add it
 505                                                         if (spot != -1) {
 506                                                                 mothurMadeFiles[it->first][spot] = temp[k];
 507                                                         }else{
 508                                                                 mothurMadeFiles[it->first].push_back(temp[k]);
 509                                                         }
 510                                                 }
 511                                         }
 512                                 }
 513
 514                                 #ifdef USE_MPI
 515                                         }
 516                                 #endif
 517                         }
 518                 }
 519
 520                 in.close();
 521         }
 522         catch(exception& e) {
 523                 m->errorOut(e, "PipelineCommand", "runUsersPipeline");
 524                 exit(1);
 525         }
 526 }
 527 //**********************************************************************************************************************
 528 bool PipelineCommand::fillInMothurMade(string& options, map<string, vector<string> > mothurMadeFiles){
 529         try {
 530                 OptionParser parser(options);
 531                 map<string, string> parameters = parser.getParameters();
 532                 map<string, string>::iterator it;
 533                 map<string, vector<string> >::iterator itMade;
 534
 535                 options = "";
 536
 537                 //fill in mothurmade filenames
 538                 for (it = parameters.begin(); it != parameters.end(); it++) {
 539                         if (it->second == "mothurmade") {
 540                                 itMade = mothurMadeFiles.find(it->first);
 541
 542                                 if (itMade == mothurMadeFiles.end()) {
 543                                         m->mothurOut("Looking for a mothurmade " + it->first + " file, but it seems mothur has not made that file type in your current pipeline, please correct."); m->mothurOutEndLine();
 544                                         return true;
 545                                 }else{
 546                                         vector<string> temp = itMade->second;
 547
 548                                         if (temp.size() > 1) {
 549                                                 //ask user which file to use
 550                                                 m->mothurOut("More than one file has been created for the " + it->first + " parameter. "); m->mothurOutEndLine();
 551                                                 for (int i = 0; i < temp.size(); i++) {
 552                                                         m->mothurOut(toString(i) + " - " + temp[i]); m->mothurOutEndLine();
 553                                                 }
 554                                                 m->mothurOut("Please select the number of the file you would like to use: ");
 555                                                 int num = 0;
 556                                                 cin >> num;
 557                                                 m->mothurOutJustToLog(toString(num)); m->mothurOutEndLine();
 558
 559                                                 if ((num < 0) || (num > (temp.size()-1))) { m->mothurOut("Not a valid response, quitting."); m->mothurOutEndLine(); return true; }
 560                                                 else {
 561                                                         parameters[it->first] = temp[num];
 562                                                 }
 563
 564                                                 //clears buffer so next command doesn't have error
 565                                                 string s;
 566                                                 getline(cin, s);
 567
 568                                         }else if (temp.size() == 0){
 569                                                 m->mothurOut("Sorry, we seem to think you created a " + it->first + " file, but it seems mothur doesn't have a filename."); m->mothurOutEndLine();
 570                                                 return true;
 571                                         }else{
 572                                                 parameters[it->first] = temp[0];
 573                                         }
 574                                 }
 575                         }
 576
 577                         options += it->first + "=" + parameters[it->first] + ", ";
 578                 }
 579
 580                 //rip off extra comma
 581                 options = options.substr(0, (options.length()-2));
 582
 583                 return false;
 584         }
 585         catch(exception& e) {
 586                 m->errorOut(e, "PipelineCommand", "fillInMothurMade");
 587                 exit(1);
 588         }
 589 }
 590
 591 //**********************************************************************************************************************
 592 void PipelineCommand::createPatsPipeline(){
 593         try {
 594
 595                 //sff.info command
 596                 string thisCommand = "sffinfo(sff=" + sffFile + ")";
 597                 //commands.push_back(thisCommand);
 598
 599                 //trim.seqs command
 600                 string fastaFile = m->getRootName(m->getSimpleName(sffFile)) + "fasta";
 601                 string qualFile = m->getRootName(m->getSimpleName(sffFile)) + "qual";
 602                 //thisCommand = "trim.seqs(processors=" + toString(processors) + ", fasta=" + fastaFile + ", allfiles=T, maxambig=0, maxhomop=8, flip=T, bdiffs=1, pdiffs=2, qwindowaverage=35, qwindowsize=50, oligos=" + oligosFile + ", qfile=" + qualFile + ")";
 603                 //commands.push_back(thisCommand);
 604
 605                 //unique.seqs
 606                 string groupFile = m->getRootName(m->getSimpleName(fastaFile)) + "groups";
 607                 qualFile =  m->getRootName(m->getSimpleName(fastaFile)) + "trim.qual";
 608                 fastaFile =  m->getRootName(m->getSimpleName(fastaFile)) + "trim.fasta";
 609                 thisCommand = "unique.seqs(fasta=" + fastaFile + ")";
 610                 commands.push_back(thisCommand);
 611
 612                 //align.seqs
 613                 string nameFile = m->getRootName(m->getSimpleName(fastaFile)) + "names";
 614                 fastaFile = m->getRootName(m->getSimpleName(fastaFile)) + "unique" + m->getExtension(fastaFile);
 615                 thisCommand = "align.seqs(processors=" + toString(processors) + ", candidate=" + fastaFile + ", template=" + alignFile + ")";
 616                 commands.push_back(thisCommand);
 617
 618                 //screen.seqs
 619                 fastaFile = m->getRootName(m->getSimpleName(fastaFile)) + "align";
 620                 thisCommand = "screen.seqs(processors=" + toString(processors) + ", fasta=" + fastaFile + ", name=" + nameFile + ", group=" + groupFile + ", optimize=end-minlength)";
 621                 commands.push_back(thisCommand);
 622
 623                 //chimera.slayer
 624                 fastaFile = m->getRootName(m->getSimpleName(fastaFile)) + "good" + m->getExtension(fastaFile);
 625                 nameFile = m->getRootName(m->getSimpleName(nameFile)) + "good" + m->getExtension(nameFile);
 626                 groupFile = m->getRootName(m->getSimpleName(groupFile)) + "good" + m->getExtension(groupFile);
 627                 thisCommand = "chimera.slayer(processors=" + toString(processors) + ", fasta=" + fastaFile + ", template=" + chimeraFile + ")";
 628                 commands.push_back(thisCommand);
 629
 630                 //remove.seqs
 631                 string accnosFile = m->getRootName(m->getSimpleName(fastaFile))  + "slayer.accnos";
 632                 thisCommand = "remove.seqs(fasta=" + fastaFile + ", name=" + nameFile + ", group=" + groupFile + ", accnos=" + accnosFile + ", dups=T)";
 633                 commands.push_back(thisCommand);
 634
 635                 //filter.seqs
 636                 nameFile = m->getRootName(m->getSimpleName(nameFile)) + "pick" + m->getExtension(nameFile);
 637                 groupFile = m->getRootName(m->getSimpleName(groupFile)) + "pick" + m->getExtension(groupFile);
 638                 fastaFile = m->getRootName(m->getSimpleName(fastaFile)) + "pick" + m->getExtension(fastaFile);
 639                 thisCommand = "filter.seqs(processors=" + toString(processors) + ", fasta=" + fastaFile + ", vertical=T, trump=.)";
 640                 commands.push_back(thisCommand);
 641
 642                 //unique.seqs
 643                 fastaFile =  m->getRootName(m->getSimpleName(fastaFile)) + "filter.fasta";
 644                 thisCommand = "unique.seqs(fasta=" + fastaFile + ", name=" + nameFile + ")";
 645                 commands.push_back(thisCommand);
 646
 647                 //pre.cluster
 648                 nameFile = m->getRootName(m->getSimpleName(fastaFile)) + "names";
 649                 fastaFile = m->getRootName(m->getSimpleName(fastaFile)) + "unique" + m->getExtension(fastaFile);
 650                 thisCommand = "pre.cluster(fasta=" + fastaFile + ", name=" + nameFile + ", diffs=2)";
 651                 commands.push_back(thisCommand);
 652
 653                 //dist.seqs
 654                 nameFile = m->getRootName(m->getSimpleName(fastaFile)) + "precluster.names";
 655                 fastaFile = m->getRootName(m->getSimpleName(fastaFile)) + "precluster" + m->getExtension(fastaFile);
 656                 thisCommand = "dist.seqs(processors=" + toString(processors) + ", fasta=" + fastaFile + ", cutoff=0.20)";
 657                 commands.push_back(thisCommand);
 658
 659                 //dist.seqs
 660                 string columnFile = m->getRootName(m->getSimpleName(fastaFile)) + "dist";
 661                 thisCommand = "dist.seqs(processors=" + toString(processors) + ", fasta=" + fastaFile + ", output=lt)";
 662                 commands.push_back(thisCommand);
 663
 664                 //read.dist
 665                 string phylipFile = m->getRootName(m->getSimpleName(fastaFile)) + "phylip.dist";
 666                 thisCommand = "read.dist(column=" + columnFile + ", name=" + nameFile + ")";
 667                 commands.push_back(thisCommand);
 668
 669                 //cluster
 670                 thisCommand = "cluster(method=average, hard=T)";
 671                 commands.push_back(thisCommand);
 672
 673                 string listFile = m->getRootName(m->getSimpleName(columnFile)) + "an.list";
 674                 string rabundFile = m->getRootName(m->getSimpleName(columnFile)) + "an.rabund";
 675
 676                 //degap.seqs
 677                 thisCommand = "degap.seqs(fasta=" + fastaFile + ")";
 678                 commands.push_back(thisCommand);
 679
 680                 //classify.seqs
 681                 fastaFile = m->getRootName(m->getSimpleName(fastaFile)) + "ng.fasta";
 682                 thisCommand = "classify.seqs(processors=" + toString(processors) + ", fasta=" + fastaFile + ", name=" + nameFile + ", template=" + classifyFile + ", taxonomy=" + taxonomyFile + ", cutoff=80)";
 683                 commands.push_back(thisCommand);
 684
 685                 string RippedTaxName = m->getRootName(m->getSimpleName(taxonomyFile));
 686                 RippedTaxName = m->getExtension(RippedTaxName.substr(0, RippedTaxName.length()-1));
 687                 if (RippedTaxName[0] == '.') { RippedTaxName = RippedTaxName.substr(1, RippedTaxName.length()); }
 688                 RippedTaxName +=  ".";
 689
 690                 string fastaTaxFile = m->getRootName(m->getSimpleName(fastaFile)) + RippedTaxName + "taxonomy";
 691                 string taxSummaryFile = m->getRootName(m->getSimpleName(fastaFile)) + RippedTaxName + "tax.summary";
 692
 693                 //phylotype
 694                 thisCommand = "phylotype(taxonomy=" + fastaTaxFile + ", name=" + nameFile + ")";
 695                 commands.push_back(thisCommand);
 696
 697                 string phyloListFile = m->getRootName(m->getSimpleName(fastaTaxFile)) + "tx.list";
 698                 string phyloRabundFile = m->getRootName(m->getSimpleName(fastaTaxFile)) + "tx.rabund";
 699
 700                 //clearcut
 701                 thisCommand = "clearcut(phylip=" + phylipFile + ", neighbor=T)";
 702                 commands.push_back(thisCommand);
 703
 704                 string treeFile = m->getRootName(m->getSimpleName(phylipFile)) + "tre";
 705
 706                 //read.otu
 707                 thisCommand = "read.otu(list=" + listFile + ", group=" + groupFile + ", label=0.03)";
 708                 commands.push_back(thisCommand);
 709
 710                 string sharedFile = m->getRootName(m->getSimpleName(listFile)) + "shared";
 711
 712                 //read.otu
 713                 thisCommand = "read.otu(list=" + phyloListFile + ", group=" + groupFile + ", label=1)";
 714                 commands.push_back(thisCommand);
 715
 716                 string phyloSharedFile = m->getRootName(m->getSimpleName(phyloListFile)) + "shared";
 717
 718                 //read.otu
 719                 thisCommand = "read.otu(shared=" + sharedFile + ")";
 720                 commands.push_back(thisCommand);
 721
 722                 //summary.single
 723                 thisCommand = "summary.single(calc=nseqs-sobs-coverage-bergerparker-chao-ace-jack-bootstrap-boneh-efron-shen-solow-shannon-npshannon-invsimpson-qstat-simpsoneven-shannoneven-heip-smithwilson, size=5000)";
 724                 commands.push_back(thisCommand);
 725
 726                 //summary.shared
 727                 thisCommand = "summary.shared(calc=sharednseqs-sharedsobs-sharedchao-sharedace-anderberg-jclass-jest-kulczynski-kulczynskicody-lennon-ochiai-sorclass-sorest-whittaker-braycurtis-jabund-morisitahorn-sorabund-thetan-thetayc)";
 728                 commands.push_back(thisCommand);
 729
 730                 //read.otu
 731                 thisCommand = "read.otu(rabund=" + rabundFile + ", label=0.03)";
 732                 commands.push_back(thisCommand);
 733
 734                 //summary.single
 735                 thisCommand = "summary.single(calc=nseqs-sobs-coverage-bergerparker-chao-ace-jack-bootstrap-boneh-efron-shen-solow-shannon-npshannon-invsimpson-qstat-simpsoneven-shannoneven-heip-smithwilson, size=5000)";
 736                 commands.push_back(thisCommand);
 737
 738                 //read.otu
 739                 thisCommand = "read.otu(shared=" + phyloSharedFile + ")";
 740                 commands.push_back(thisCommand);
 741
 742                 //summary.single
 743                 thisCommand = "summary.single(calc=nseqs-sobs-coverage-bergerparker-chao-ace-jack-bootstrap-boneh-efron-shen-solow-shannon-npshannon-invsimpson-qstat-simpsoneven-shannoneven-heip-smithwilson, size=5000)";
 744                 commands.push_back(thisCommand);
 745
 746                 //summary.shared
 747                 thisCommand = "summary.shared(calc=sharednseqs-sharedsobs-sharedchao-sharedace-anderberg-jclass-jest-kulczynski-kulczynskicody-lennon-ochiai-sorclass-sorest-whittaker-braycurtis-jabund-morisitahorn-sorabund-thetan-thetayc)";
 748                 commands.push_back(thisCommand);
 749
 750                 //read.otu
 751                 thisCommand = "read.otu(rabund=" + phyloRabundFile + ", label=1)";
 752                 commands.push_back(thisCommand);
 753
 754                 //summary.single
 755                 thisCommand = "summary.single(calc=nseqs-sobs-coverage-bergerparker-chao-ace-jack-bootstrap-boneh-efron-shen-solow-shannon-npshannon-invsimpson-qstat-simpsoneven-shannoneven-heip-smithwilson, size=5000)";
 756                 commands.push_back(thisCommand);
 757
 758                 //classify.otu
 759                 thisCommand = "classify.otu(taxonomy=" + fastaTaxFile + ", name=" + nameFile + ", list=" + listFile + ", cutoff=51, label=0.03)";
 760                 commands.push_back(thisCommand);
 761
 762                 //classify.otu
 763                 thisCommand = "classify.otu(taxonomy=" + fastaTaxFile + ", name=" + nameFile + ", list=" + phyloListFile + ", cutoff=51, label=1)";
 764                 commands.push_back(thisCommand);
 765
 766                 //read.tree
 767                 thisCommand = "read.tree(tree=" + treeFile + ", name=" + nameFile + ", group=" + groupFile + ")";
 768                 commands.push_back(thisCommand);
 769
 770                 //phylo.diversity
 771                 thisCommand = "phylo.diversity(iters=100,rarefy=T)";
 772                 commands.push_back(thisCommand);
 773
 774                 //unifrac.weighted
 775                 thisCommand = "unifrac.weighted(random=false, distance=true, groups=all, processors=" + toString(processors) + ")";
 776                 commands.push_back(thisCommand);
 777
 778                 //unifrac.unweighted
 779                 thisCommand = "unifrac.unweighted(random=false, distance=true, processors=" + toString(processors) + ")";
 780                 commands.push_back(thisCommand);
 781
 782
 783         }
 784         catch(exception& e) {
 785                 m->errorOut(e, "PipelineCommand", "createPatsPipeline");
 786                 exit(1);
 787         }
 788 }
 789
 790 //**********************************************************************************************************************