metastatscommand.h

   1 #ifndef METASTATSCOMMAND_H
   2 #define METASTATSCOMMAND_H
   3
   4 /*
   5  *  metastatscommand.h
   6  *  Mothur
   7  *
   8  *  Created by westcott on 9/16/10.
   9  *  Copyright 2010 Schloss Lab. All rights reserved.
  10  *
  11  */
  12
  13 #include "command.hpp"
  14 #include "inputdata.h"
  15 #include "sharedrabundvector.h"
  16 #include "mothurmetastats.h"
  17
  18 class MetaStatsCommand : public Command {
  19
  20 public:
  21         MetaStatsCommand(string);
  22         MetaStatsCommand();
  23         ~MetaStatsCommand() {}
  24
  25         vector<string> setParameters();
  26         string getCommandName()                 { return "metastats";                           }
  27         string getCommandCategory()             { return "OTU-Based Approaches";        }
  28         string getOutputFileNameTag(string, string);
  29         string getHelpString();
  30         string getCitation() { return "White JR, Nagarajan N, Pop M (2009). Statistical methods for detecting differentially abundant features in clinical metagenomic samples. PLoS Comput Biol 5: e1000352. \nhttp://www.mothur.org/wiki/Metastats"; }
  31         string getDescription()         { return "detects differentially abundant features in clinical metagenomic samples"; }
  32
  33         int execute();
  34         void help() { m->mothurOut(getHelpString()); }
  35
  36 private:
  37         struct linePair {
  38                 int start;
  39                 int num;
  40                 linePair(int i, int j) : start(i), num(j) {}
  41         };
  42         vector<linePair> lines;
  43
  44         GroupMap* designMap;
  45         InputData* input;
  46         vector<SharedRAbundVector*> lookup;
  47
  48         bool abort, allLines, pickedGroups;
  49         set<string> labels; //holds labels to be used
  50         string groups, label, outputDir, inputDir, designfile, sets, sharedfile;
  51         vector<string> Groups, outputNames, Sets;
  52         vector< vector<string> > namesOfGroupCombos;
  53         int iters, processors;
  54         float threshold;
  55
  56         int process(vector<SharedRAbundVector*>&);
  57         int driver(int, int, vector<SharedRAbundVector*>&);
  58 };
  59
  60 /**************************************************************************************************/
  61 //custom data structure for threads to use.
  62 // This is passed by void pointer so it can be any data type
  63 // that can be passed using a single void pointer (LPVOID).
  64 struct metastatsData {
  65     vector<SharedRAbundVector*> thisLookUp;
  66     vector< vector<string> > namesOfGroupCombos;
  67     vector<string> designMapGroups;
  68     vector<string> outputNames;
  69         int start;
  70         int num, iters;
  71         float threshold;
  72         MothurOut* m;
  73         string sharedfile;
  74     string outputDir;
  75
  76         metastatsData(){}
  77         metastatsData(string sf, string oDir, MothurOut* mout, int st, int en, vector< vector<string> > ns, vector<SharedRAbundVector*> lu, vector<string> dg, int i, float thr) {
  78                 sharedfile = sf;
  79         outputDir = oDir;
  80                 m = mout;
  81                 start = st;
  82                 num = en;
  83         namesOfGroupCombos = ns;
  84         thisLookUp = lu;
  85         designMapGroups = dg;
  86         iters = i;
  87         threshold = thr;
  88         }
  89 };
  90 /**************************************************************************************************/
  91 #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
  92 #else
  93 static DWORD WINAPI MyMetastatsThreadFunction(LPVOID lpParam){
  94         metastatsData* pDataArray;
  95         pDataArray = (metastatsData*)lpParam;
  96
  97         try {
  98
  99         //for each combo
 100                 for (int c = pDataArray->start; c < (pDataArray->start+pDataArray->num); c++) {
 101
 102                         //get set names
 103                         string setA = pDataArray->namesOfGroupCombos[c][0];
 104                         string setB = pDataArray->namesOfGroupCombos[c][1];
 105
 106                         //get filename
 107                         string outputFileName = pDataArray->outputDir +  pDataArray->m->getRootName(pDataArray->m->getSimpleName(pDataArray->sharedfile)) + pDataArray->thisLookUp[0]->getLabel() + "." + setA + "-" + setB + ".metastats";
 108                         pDataArray->outputNames.push_back(outputFileName);
 109
 110                         vector< vector<double> > data2; data2.resize(pDataArray->thisLookUp[0]->getNumBins());
 111
 112                         vector<SharedRAbundVector*> subset;
 113                         int setACount = 0;
 114                         int setBCount = 0;
 115                         for (int i = 0; i < pDataArray->thisLookUp.size(); i++) {
 116                                 //is this group for a set we want to compare??
 117                                 //sorting the sets by putting setB at the back and setA in the front
 118                                 if (pDataArray->designMapGroups[i] == setB) {
 119                                         subset.push_back(pDataArray->thisLookUp[i]);
 120                                         setBCount++;
 121                                 }else if (pDataArray->designMapGroups[i] == setA) {
 122                                         subset.insert(subset.begin()+setACount, pDataArray->thisLookUp[i]);
 123                                         setACount++;
 124                                 }
 125                         }
 126
 127                         if ((setACount == 0) || (setBCount == 0))  {
 128                                 pDataArray->m->mothurOut("Missing shared info for " + setA + " or " + setB + ". Skipping comparison."); pDataArray->m->mothurOutEndLine();
 129                                 pDataArray->outputNames.pop_back();
 130                         }else {
 131                                 //fill data
 132                                 for (int j = 0; j < pDataArray->thisLookUp[0]->getNumBins(); j++) {
 133                                         data2[j].resize(subset.size(), 0.0);
 134                                         for (int i = 0; i < subset.size(); i++) {
 135                                                 data2[j][i] = (subset[i]->getAbundance(j));
 136                                         }
 137                                 }
 138
 139                                 pDataArray->m->mothurOut("Comparing " + setA + " and " + setB + "..."); pDataArray->m->mothurOutEndLine();
 140
 141                                 pDataArray->m->mothurOutEndLine();
 142                                 MothurMetastats mothurMeta(pDataArray->threshold, pDataArray->iters);
 143                                 mothurMeta.runMetastats(outputFileName, data2, setACount);
 144                                 pDataArray->m->mothurOutEndLine();
 145                                 pDataArray->m->mothurOutEndLine();
 146                         }
 147         }
 148
 149                 return 0;
 150
 151         }
 152         catch(exception& e) {
 153                 pDataArray->m->errorOut(e, "MetaStatsCommand", "MyMetastatsThreadFunction");
 154                 exit(1);
 155         }
 156 }
 157 #endif
 158
 159
 160
 161 #endif
 162