maligner.cpp

   1 /*
   2  *  maligner.cpp
   3  *  Mothur
   4  *
   5  *  Created by westcott on 9/23/09.
   6  *  Copyright 2009 Schloss Lab. All rights reserved.
   7  *
   8  */
   9
  10 #include "maligner.h"
  11
  12 /***********************************************************************/ //int num, int match, int misMatch, , string mode, Database* dataLeft, Database* dataRight
  13 Maligner::Maligner(vector<Sequence*> temp, int match, int misMatch, float div, int ms, int minCov) : db(temp), matchScore(match), misMatchPenalty(misMatch), minDivR(div), minSimilarity(ms), minCoverage(minCov) {
  14                         //numWanted(num),  , searchMethod(mode), databaseLeft(dataLeft), databaseRight(dataRight)
  15
  16                         m = MothurOut::getInstance();
  17
  18 }
  19 /***********************************************************************/
  20 string Maligner::getResults(Sequence* q, DeCalculator* decalc) {
  21         try {
  22
  23                 outputResults.clear();
  24
  25                 //make copy so trimming doesn't destroy query from calling class - remember to deallocate
  26                 query = new Sequence(q->getName(), q->getAligned());
  27
  28                 string chimera;
  29
  30                 //copy refSeqs so that filter does not effect original
  31                 for(int i = 0; i < db.size(); i++) {
  32                         Sequence* newSeq = new Sequence(db[i]->getName(), db[i]->getAligned());
  33                         refSeqs.push_back(newSeq);
  34                 }
  35
  36                 refSeqs = minCoverageFilter(refSeqs);
  37
  38                 if (refSeqs.size() < 2)  {
  39                         for (int i = 0; i < refSeqs.size(); i++) {  delete refSeqs[i];  }
  40                         percentIdenticalQueryChimera = 0.0;
  41                         return "unknown";
  42                 }
  43
  44                 int chimeraPenalty = computeChimeraPenalty();
  45
  46                 //fills outputResults
  47                 chimera = chimeraMaligner(chimeraPenalty, decalc);
  48
  49                 if (m->control_pressed) { return chimera;  }
  50
  51                 //free memory
  52                 delete query;
  53
  54                 for (int i = 0; i < refSeqs.size(); i++) {  delete refSeqs[i];  }
  55
  56                 return chimera;
  57         }
  58         catch(exception& e) {
  59                 m->errorOut(e, "Maligner", "getResults");
  60                 exit(1);
  61         }
  62 }
  63 /***********************************************************************/
  64 string Maligner::chimeraMaligner(int chimeraPenalty, DeCalculator* decalc) {
  65         try {
  66
  67                 string chimera;
  68
  69                 //trims seqs to first non gap char in all seqs and last non gap char in all seqs
  70                 spotMap = decalc->trimSeqs(query, refSeqs);
  71
  72                 //you trimmed the whole sequence, skip
  73                 if (query->getAligned() == "") { return "no"; }
  74
  75                 vector<Sequence*> temp = refSeqs;
  76
  77 //              for(int i=0;i<refSeqs.size();i++){
  78 //                      cout << refSeqs[i]->getName() << endl;
  79 //              }
  80
  81                 temp.push_back(query);
  82
  83                 verticalFilter(temp);
  84
  85                 //for (int i = 0; i < refSeqs.size(); i++) { cout << refSeqs[i]->getName() << endl ; }//<< refSeqs[i]->getAligned() << endl
  86
  87                 vector< vector<score_struct> > matrix = buildScoreMatrix(query->getAligned().length(), refSeqs.size()); //builds and initializes
  88
  89                 if (m->control_pressed) { return chimera;  }
  90
  91                 fillScoreMatrix(matrix, refSeqs, chimeraPenalty);
  92
  93                 vector<score_struct> path = extractHighestPath(matrix);
  94
  95                 if (m->control_pressed) { return chimera;  }
  96
  97                 vector<trace_struct> trace = mapTraceRegionsToAlignment(path, refSeqs);
  98
  99                 if (trace.size() > 1) {         chimera = "yes";        }
 100                 else { chimera = "no";  return chimera; }
 101
 102                 int traceStart = path[0].col;
 103                 int traceEnd = path[path.size()-1].col;
 104                 string queryInRange = query->getAligned();
 105                 queryInRange = queryInRange.substr(traceStart, (traceEnd-traceStart+1));
 106 //              cout << queryInRange << endl;
 107                 string chimeraSeq = constructChimericSeq(trace, refSeqs);
 108 //              cout << chimeraSeq << endl;
 109
 110 //              cout << queryInRange.length() << endl;
 111 //              cout << chimeraSeq.length() << endl;
 112
 113                 percentIdenticalQueryChimera = computePercentID(queryInRange, chimeraSeq);
 114
 115 //              cout << percentIdenticalQueryChimera << endl;
 116         /*
 117                 vector<trace_struct> trace = extractHighestPath(matrix);
 118
 119                 //cout << "traces\n";
 120                 //for(int i=0;i<trace.size();i++){
 121                 //      cout << trace[i].col << '\t' << trace[i].oldCol << '\t' << refSeqs[trace[i].row]->getName() << endl;
 122                 //}
 123
 124                 if (trace.size() > 1) {         chimera = "yes";        }
 125                 else { chimera = "no";  return chimera; }
 126
 127                 int traceStart = trace[0].col;
 128                 int traceEnd = trace[trace.size()-1].oldCol;
 129                 string queryInRange = query->getAligned();
 130                 queryInRange = queryInRange.substr(traceStart, (traceEnd-traceStart));*/
 131
 132                 if (m->control_pressed) { return chimera;  }
 133
 134                 //save output results
 135                 for (int i = 0; i < trace.size(); i++) {
 136                         int regionStart = trace[i].col;
 137                         int regionEnd = trace[i].oldCol;
 138                         int seqIndex = trace[i].row;
 139
 140 //                      cout << regionStart << '\t' << regionEnd << '\t' << seqIndex << endl;
 141                         results temp;
 142
 143                         temp.parent = refSeqs[seqIndex]->getName();
 144                         temp.parentAligned = db[seqIndex]->getAligned();
 145                         temp.nastRegionStart = spotMap[regionStart];
 146                         temp.nastRegionEnd = spotMap[regionEnd];
 147                         temp.regionStart = regionStart;
 148                         temp.regionEnd = regionEnd;
 149
 150                         string parentInRange = refSeqs[seqIndex]->getAligned();
 151                         parentInRange = parentInRange.substr(traceStart, (traceEnd-traceStart+1));
 152
 153                         temp.queryToParent = computePercentID(queryInRange, parentInRange);
 154                         temp.divR = (percentIdenticalQueryChimera / temp.queryToParent);
 155
 156                         string queryInRegion = query->getAligned();
 157                         queryInRegion = queryInRegion.substr(regionStart, (regionEnd-regionStart+1));
 158
 159                         string parentInRegion = refSeqs[seqIndex]->getAligned();
 160                         parentInRegion = parentInRegion.substr(regionStart, (regionEnd-regionStart+1));
 161
 162                         temp.queryToParentLocal = computePercentID(queryInRegion, parentInRegion);
 163
 164 //                      cout << query->getName() << '\t' << temp.parent << '\t' << "NAST:" << temp.nastRegionStart << '-' << temp.nastRegionEnd << " G:" << temp.queryToParent << " L:" << temp.queryToParentLocal << ", " <<  temp.divR << endl;
 165
 166                         outputResults.push_back(temp);
 167                 }
 168
 169                 return chimera;
 170         }
 171         catch(exception& e) {
 172                 m->errorOut(e, "Maligner", "chimeraMaligner");
 173                 exit(1);
 174         }
 175 }
 176 /***********************************************************************/
 177 //removes top matches that do not have minimum coverage with query.
 178 vector<Sequence*> Maligner::minCoverageFilter(vector<Sequence*> ref){
 179         try {
 180                 vector<Sequence*> newRefs;
 181
 182                 string queryAligned = query->getAligned();
 183
 184                 for (int i = 0; i < ref.size(); i++) {
 185
 186                         string refAligned = ref[i]->getAligned();
 187
 188                         int numBases = 0;
 189                         int numCovered = 0;
 190
 191                         //calculate coverage
 192                         for (int j = 0; j < queryAligned.length(); j++) {
 193
 194                                 if (isalpha(queryAligned[j])) {
 195                                         numBases++;
 196
 197                                         if (isalpha(refAligned[j])) {
 198                                                 numCovered++;
 199                                         }
 200                                 }
 201                         }
 202
 203                         int coverage = ((numCovered/(float)numBases)*100);
 204
 205                         //if coverage above minimum
 206                         if (coverage > minCoverage) {
 207                                 newRefs.push_back(ref[i]);
 208                         }else {
 209                                 delete ref[i];
 210                         }
 211                 }
 212
 213                 return newRefs;
 214         }
 215         catch(exception& e) {
 216                 m->errorOut(e, "Maligner", "minCoverageFilter");
 217                 exit(1);
 218         }
 219 }
 220 /***********************************************************************/
 221 // a breakpoint should yield fewer mismatches than this number with respect to the best parent sequence.
 222 int Maligner::computeChimeraPenalty() {
 223         try {
 224
 225                 int numAllowable = ((1.0 - (1.0/minDivR)) * query->getNumBases());
 226
 227 //              if(numAllowable < 1){   numAllowable = 1;       }
 228
 229                 int penalty = int(numAllowable + 1) * misMatchPenalty;
 230
 231                 return penalty;
 232
 233         }
 234         catch(exception& e) {
 235                 m->errorOut(e, "Maligner", "computeChimeraPenalty");
 236                 exit(1);
 237         }
 238 }
 239 /***********************************************************************/
 240 //this is a vertical filter
 241 void Maligner::verticalFilter(vector<Sequence*> seqs) {
 242         try {
 243                 vector<int> gaps;       gaps.resize(query->getAligned().length(), 0);
 244
 245                 string filterString = (string(query->getAligned().length(), '1'));
 246
 247                 //for each sequence
 248                 for (int i = 0; i < seqs.size(); i++) {
 249
 250                         string seqAligned = seqs[i]->getAligned();
 251
 252                         for (int j = 0; j < seqAligned.length(); j++) {
 253                                 //if this spot is a gap
 254                                 if ((seqAligned[j] == '-') || (seqAligned[j] == '.'))   {       gaps[j]++;      }
 255                         }
 256                 }
 257
 258                 //zero out spot where all sequences have blanks
 259                 int numColRemoved = 0;
 260                 for(int i = 0; i < seqs[0]->getAligned().length(); i++){
 261                         if(gaps[i] == seqs.size())      {       filterString[i] = '0';  numColRemoved++;  }
 262                 }
 263
 264                 map<int, int> newMap;
 265                 //for each sequence
 266                 for (int i = 0; i < seqs.size(); i++) {
 267
 268                         string seqAligned = seqs[i]->getAligned();
 269                         string newAligned = "";
 270                         int count = 0;
 271
 272                         for (int j = 0; j < seqAligned.length(); j++) {
 273                                 //if this spot is not a gap
 274                                 if (filterString[j] == '1') {
 275                                         newAligned += seqAligned[j];
 276                                         newMap[count] = spotMap[j];
 277                                         count++;
 278                                 }
 279                         }
 280
 281                         seqs[i]->setAligned(newAligned);
 282                 }
 283
 284                 spotMap = newMap;
 285         }
 286         catch(exception& e) {
 287                 m->errorOut(e, "Maligner", "verticalFilter");
 288                 exit(1);
 289         }
 290 }
 291 //***************************************************************************************************************
 292 vector< vector<score_struct> > Maligner::buildScoreMatrix(int cols, int rows) {
 293         try{
 294
 295                 vector< vector<score_struct> > m(rows);
 296
 297                 for (int i = 0; i < rows; i++) {
 298                         for (int j = 0; j < cols; j++) {
 299
 300                                 //initialize each cell
 301                                 score_struct temp;
 302                                 temp.prev = -1;
 303                                 temp.score = -9999999;
 304                                 temp.col = j;
 305                                 temp.row = i;
 306
 307                                 m[i].push_back(temp);
 308                         }
 309                 }
 310
 311                 return m;
 312         }
 313         catch(exception& e) {
 314                 m->errorOut(e, "Maligner", "buildScoreMatrix");
 315                 exit(1);
 316         }
 317 }
 318
 319 //***************************************************************************************************************
 320
 321 void Maligner::fillScoreMatrix(vector<vector<score_struct> >& ms, vector<Sequence*> seqs, int penalty) {
 322         try{
 323
 324                 //get matrix dimensions
 325                 int numCols = query->getAligned().length();
 326                 int numRows = seqs.size();
 327
 328 //              cout << numRows << endl;
 329
 330                 //initialize first col
 331                 string queryAligned = query->getAligned();
 332                 for (int i = 0; i < numRows; i++) {
 333                         string subjectAligned = seqs[i]->getAligned();
 334
 335                         //are you both gaps?
 336                         if ((!isalpha(queryAligned[0])) && (!isalpha(subjectAligned[0]))) {
 337                                 ms[i][0].score = 0;
 338 //                              ms[i][0].mismatches = 0;
 339                         }else if (queryAligned[0] == subjectAligned[0])  { //|| subjectAligned[0] == 'N')
 340                                 ms[i][0].score = matchScore;
 341 //                              ms[i][0].mismatches = 0;
 342                         }else{
 343                                 ms[i][0].score = 0;
 344 //                              ms[i][0].mismatches = 1;
 345                         }
 346                 }
 347
 348                 //fill rest of matrix
 349                 for (int j = 1; j < numCols; j++) {  //iterate through matrix columns
 350
 351 //                      for (int i = 0; i < 1; i++) {  //iterate through matrix rows
 352                         for (int i = 0; i < numRows; i++) {  //iterate through matrix rows
 353
 354                                 string subjectAligned = seqs[i]->getAligned();
 355
 356                                 int matchMisMatchScore = 0;
 357                                 //are you both gaps?
 358                                 if ((!isalpha(queryAligned[j])) && (!isalpha(subjectAligned[j]))) {
 359                                         //leave the same
 360                                 }else if ((toupper(queryAligned[j]) == 'N') || (toupper(subjectAligned[j]) == 'N')) {
 361                                         //leave the same
 362                                 }else if (queryAligned[j] == subjectAligned[j]) {
 363                                         matchMisMatchScore = matchScore;
 364                                 }else if (queryAligned[j] != subjectAligned[j]) {
 365                                         matchMisMatchScore = misMatchPenalty;
 366                                 }
 367
 368                                 //compute score based on previous columns scores
 369                                 for (int prevIndex = 0; prevIndex < numRows; prevIndex++) { //iterate through rows
 370
 371                                         int sumScore = matchMisMatchScore + ms[prevIndex][j-1].score;
 372
 373                                         //you are not at yourself
 374                                         if (prevIndex != i) {   sumScore += penalty;    }
 375                                         if (sumScore < 0)       {       sumScore = 0;                   }
 376
 377                                         if (sumScore > ms[i][j].score) {
 378                                                 ms[i][j].score = sumScore;
 379                                                 ms[i][j].prev = prevIndex;
 380                                         }
 381                                 }
 382 //                              cout << i << '\t' << j << '\t' << queryAligned[j] << '\t' << subjectAligned[j] << '\t' << matchMisMatchScore << '\t' << ms[i][j].score << endl;
 383
 384                         }
 385
 386                 }
 387
 388
 389
 390
 391
 392 //              cout << numRows << '\t' << numCols << endl;
 393 //              for(int i=0;i<numRows;i++){
 394 //                      cout << seqs[i]->getName();
 395 //                      for(int j=0;j<numCols;j++){
 396 //                              cout << '\t' << ms[i][j].score;
 397 //                      }
 398 //                      cout << endl;
 399 //              }
 400 //              cout << endl;
 401 //
 402 //              for(int i=0;i<numRows;i++){
 403 //                      cout << seqs[i]->getName();
 404 //                      for(int j=0;j<numCols;j++){
 405 //                              cout << '\t' << ms[i][j].prev;
 406 //                      }
 407 //                      cout << endl;
 408 //              }
 409
 410
 411         }
 412         catch(exception& e) {
 413                 m->errorOut(e, "Maligner", "fillScoreMatrix");
 414                 exit(1);
 415         }
 416 }
 417 //***************************************************************************************************************
 418 vector<score_struct> Maligner::extractHighestPath(vector<vector<score_struct> > ms) {
 419         try {
 420
 421                 //get matrix dimensions
 422                 int numCols = query->getAligned().length();
 423                 int numRows = ms.size();
 424
 425
 426                 //find highest score scoring matrix
 427                 score_struct highestStruct;
 428                 int highestScore = 0;
 429
 430                 for (int i = 0; i < numRows; i++) {
 431                         for (int j = 0; j < numCols; j++) {
 432                                 if (ms[i][j].score > highestScore) {
 433                                         highestScore = ms[i][j].score;
 434                                         highestStruct = ms[i][j];
 435                                 }
 436                         }
 437                 }
 438
 439 //              cout << highestScore << endl;
 440                 vector<score_struct> path;
 441
 442                 int rowIndex = highestStruct.row;
 443                 int pos = highestStruct.col;
 444                 int score = highestStruct.score;
 445
 446                 while (pos >= 0 && score > 0) {
 447                         score_struct temp = ms[rowIndex][pos];
 448                         score = temp.score;
 449
 450                         if (score > 0) {        path.push_back(temp);   }
 451
 452                         rowIndex = temp.prev;
 453                         pos--;
 454                 }
 455
 456                 reverse(path.begin(), path.end());
 457
 458                 return path;
 459
 460         }
 461         catch(exception& e) {
 462                 m->errorOut(e, "Maligner", "extractHighestPath");
 463                 exit(1);
 464         }
 465 }
 466 //***************************************************************************************************************
 467 vector<trace_struct> Maligner::mapTraceRegionsToAlignment(vector<score_struct> path, vector<Sequence*> seqs) {
 468         try {
 469                 vector<trace_struct> trace;
 470
 471                 int region_index = path[0].row;
 472                 int region_start = path[0].col;
 473
 474                 for (int i = 1; i < path.size(); i++) {
 475
 476                         int next_region_index = path[i].row;
 477 //                      cout << i << '\t' << next_region_index << endl;
 478
 479                         if (next_region_index != region_index) {
 480
 481                                 // add trace region
 482                                 int col_index = path[i].col;
 483                                 trace_struct temp;
 484                                 temp.col = region_start;
 485                                 temp.oldCol = col_index-1;
 486                                 temp.row = region_index;
 487
 488                                 trace.push_back(temp);
 489
 490                                 region_index = path[i].row;
 491                                 region_start = col_index;
 492                         }
 493                 }
 494
 495                 // get last one
 496                 trace_struct temp;
 497                 temp.col = region_start;
 498                 temp.oldCol = path[path.size()-1].col;
 499                 temp.row = region_index;
 500                 trace.push_back(temp);
 501
 502                 return trace;
 503
 504         }
 505         catch(exception& e) {
 506                 m->errorOut(e, "Maligner", "mapTraceRegionsToAlignment");
 507                 exit(1);
 508         }
 509 }
 510
 511 /***************************************************************************************************************
 512
 513 vector<trace_struct> Maligner::extractHighestPath(vector<vector<score_struct> > ms) {
 514         try {
 515
 516
 517                 //get matrix dimensions
 518                 int numCols = query->getAligned().length();
 519                 int numRows = ms.size();
 520
 521
 522                 //find highest score scoring matrix
 523                 vector<score_struct> highestStruct;
 524                 int highestScore = 0;
 525
 526                 for (int i = 0; i < numRows; i++) {
 527                         for (int j = 0; j < numCols; j++) {
 528                                 if (ms[i][j].score > highestScore) {
 529                                         highestScore = ms[i][j].score;
 530                                         highestStruct.resize(0);
 531                                         highestStruct.push_back(ms[i][j]);
 532                                 }
 533                                 else if(ms[i][j].score == highestScore){
 534                                         highestStruct.push_back(ms[i][j]);
 535                                 }
 536                         }
 537                 }
 538
 539                 //cout << endl << highestScore << '\t' << highestStruct.size() << '\t' << highestStruct[0].row << endl;
 540
 541                 vector<trace_struct> maxTrace;
 542                 double maxPercentIdenticalQueryAntiChimera = 0;
 543
 544                 for(int i=0;i<highestStruct.size();i++){
 545
 546                         vector<score_struct> path;
 547
 548                         int rowIndex = highestStruct[i].row;
 549                         int pos = highestStruct[i].col;
 550                         int score = highestStruct[i].score;
 551
 552                         while (pos >= 0 && score > 0) {
 553                                 score_struct temp = ms[rowIndex][pos];
 554                                 score = temp.score;
 555
 556                                 if (score > 0) {        path.push_back(temp);   }
 557
 558                                 rowIndex = temp.prev;
 559                                 pos--;
 560                         }
 561
 562                         reverse(path.begin(), path.end());
 563
 564                         vector<trace_struct> trace = mapTraceRegionsToAlignment(path, refSeqs);
 565
 566                         //cout << "traces\n";
 567                         //for(int j=0;j<trace.size();j++){
 568                         //      cout << trace[j].col << '\t' << trace[j].oldCol << '\t' << refSeqs[trace[j].row]->getName() << endl;
 569                         //}
 570
 571                         int traceStart = path[0].col;
 572                         int traceEnd = path[path.size()-1].col;
 573 //                      cout << "traceStart/End\t" << traceStart << '\t' << traceEnd << endl;
 574
 575                         string queryInRange = query->getAligned();
 576                         queryInRange = queryInRange.substr(traceStart, (traceEnd-traceStart+1));
 577 //                      cout << "here" << endl;
 578                         string chimeraSeq = constructChimericSeq(trace, refSeqs);
 579                         string antiChimeraSeq = constructAntiChimericSeq(trace, refSeqs);
 580
 581                         percentIdenticalQueryChimera = computePercentID(queryInRange, chimeraSeq);
 582                         double percentIdenticalQueryAntiChimera = computePercentID(queryInRange, antiChimeraSeq);
 583 //                      cout << i << '\t' << percentIdenticalQueryChimera << '\t' << percentIdenticalQueryAntiChimera << endl;
 584
 585                         if(percentIdenticalQueryAntiChimera > maxPercentIdenticalQueryAntiChimera){
 586                                 maxPercentIdenticalQueryAntiChimera = percentIdenticalQueryAntiChimera;
 587                                 maxTrace = trace;
 588                         }
 589                 }
 590 //              cout << maxPercentIdenticalQueryAntiChimera << endl;
 591                 return maxTrace;
 592
 593
 594         }
 595         catch(exception& e) {
 596                 m->errorOut(e, "Maligner", "extractHighestPath");
 597                 exit(1);
 598         }
 599 }
 600
 601 //***************************************************************************************************************
 602
 603 vector<trace_struct> Maligner::mapTraceRegionsToAlignment(vector<score_struct> path, vector<Sequence*> seqs) {
 604         try {
 605                 vector<trace_struct> trace;
 606
 607                 int region_index = path[0].row;
 608                 int region_start = path[0].col;
 609
 610                 for (int i = 1; i < path.size(); i++) {
 611
 612                         int next_region_index = path[i].row;
 613
 614                         if (next_region_index != region_index) {
 615
 616                                 // add trace region
 617                                 int col_index = path[i].col;
 618                                 trace_struct temp;
 619                                 temp.col = region_start;
 620                                 temp.oldCol = col_index-1;
 621                                 temp.row = region_index;
 622
 623                                 trace.push_back(temp);
 624
 625                                 region_index = path[i].row;
 626                                 region_start = col_index;
 627                         }
 628                 }
 629
 630                 // get last one
 631                 trace_struct temp;
 632                 temp.col = region_start;
 633                 temp.oldCol = path[path.size()-1].col;
 634                 temp.row = region_index;
 635                 trace.push_back(temp);
 636
 637 //              cout << endl;
 638 //              cout << trace.size() << endl;
 639 //              for(int i=0;i<trace.size();i++){
 640 //                      cout << seqs[trace[i].row]->getName() << endl;
 641 //              }
 642 //              cout << endl;
 643
 644                 return trace;
 645
 646         }
 647         catch(exception& e) {
 648                 m->errorOut(e, "Maligner", "mapTraceRegionsToAlignment");
 649                 exit(1);
 650         }
 651 }
 652 */
 653 //***************************************************************************************************************
 654
 655 string Maligner::constructChimericSeq(vector<trace_struct> trace, vector<Sequence*> seqs) {
 656         try {
 657                 string chimera = "";
 658
 659                 for (int i = 0; i < trace.size(); i++) {
 660 //                      cout << i << '\t' << trace[i].row << '\t' << trace[i].col << '\t' << trace[i].oldCol << endl;
 661
 662                         string seqAlign = seqs[trace[i].row]->getAligned();
 663                         seqAlign = seqAlign.substr(trace[i].col, (trace[i].oldCol-trace[i].col+1));
 664                         chimera += seqAlign;
 665                 }
 666 //              cout << chimera << endl;
 667 //              if (chimera != "") { chimera = chimera.substr(0, (chimera.length()-1)); }       //this was introducing a fence post error
 668 //              cout << chimera << endl;
 669                 return chimera;
 670         }
 671         catch(exception& e) {
 672                 m->errorOut(e, "Maligner", "constructChimericSeq");
 673                 exit(1);
 674         }
 675 }
 676
 677 //***************************************************************************************************************
 678
 679 string Maligner::constructAntiChimericSeq(vector<trace_struct> trace, vector<Sequence*> seqs) {
 680         try {
 681                 string antiChimera = "";
 682
 683                 for (int i = 0; i < trace.size(); i++) {
 684 //                      cout << i << '\t' << (trace.size() - i - 1) << '\t' << trace[i].row << '\t' << trace[i].col << '\t' << trace[i].oldCol << endl;
 685
 686                         int oppositeIndex = trace.size() - i - 1;
 687
 688                         string seqAlign = seqs[trace[oppositeIndex].row]->getAligned();
 689                         seqAlign = seqAlign.substr(trace[i].col, (trace[i].oldCol-trace[i].col+1));
 690                         antiChimera += seqAlign;
 691                 }
 692
 693                 return antiChimera;
 694         }
 695         catch(exception& e) {
 696                 m->errorOut(e, "Maligner", "constructChimericSeq");
 697                 exit(1);
 698         }
 699 }
 700
 701 //***************************************************************************************************************
 702 float Maligner::computePercentID(string queryAlign, string chimera) {
 703         try {
 704
 705                 if (queryAlign.length() != chimera.length()) {
 706                         m->mothurOut("Error, alignment strings are of different lengths: "); m->mothurOutEndLine();
 707                         m->mothurOut(toString(queryAlign.length())); m->mothurOutEndLine();
 708                         m->mothurOut(toString(chimera.length())); m->mothurOutEndLine();
 709                         return -1.0;
 710                 }
 711
 712 //              cout << queryAlign.length() << endl;
 713                 int numIdentical = 0;
 714                 int countA = 0;
 715                 int countB = 0;
 716                 for (int i = 0; i < queryAlign.length(); i++) {
 717                         if (((queryAlign[i] != 'G') && (queryAlign[i] != 'T') && (queryAlign[i] != 'A') && (queryAlign[i] != 'C')&& (queryAlign[i] != '.') && (queryAlign[i] != '-')) ||
 718                                 ((chimera[i] != 'G') && (chimera[i] != 'T') && (chimera[i] != 'A') && (chimera[i] != 'C')&& (chimera[i] != '.') && (chimera[i] != '-'))) {}
 719                         else {
 720
 721                                 bool charA = false; bool charB = false;
 722                                 if ((queryAlign[i] == 'G') || (queryAlign[i] == 'T') || (queryAlign[i] == 'A') || (queryAlign[i] == 'C')) { charA = true; }
 723                                 if ((chimera[i] == 'G') || (chimera[i] == 'T') || (chimera[i] == 'A') || (chimera[i] == 'C')) { charB = true; }
 724
 725
 726                                 if (charA || charB) {
 727
 728                                         if (charA) { countA++; }
 729                                         if (charB) { countB++; }
 730
 731                                         if (queryAlign[i] == chimera[i]) {
 732                                                 numIdentical++;
 733                                         }
 734                                 }
 735 //                              cout << queryAlign[i] << '\t' << chimera[i] << '\t' << countA << '\t' << countB << endl;
 736
 737                         }
 738                 }
 739
 740 //              cout << "pat\t" << countA << '\t' << countB << '\t' << numIdentical << endl;
 741
 742
 743                 float numBases = (countA + countB) /(float) 2;
 744
 745                 if (numBases == 0) { return 0; }
 746
 747 //              cout << numIdentical << '\t' << numBases << endl;
 748
 749                 float percentIdentical = (numIdentical/(float)numBases) * 100;
 750
 751 //              cout << percentIdentical << endl;
 752
 753                 return percentIdentical;
 754
 755         }
 756         catch(exception& e) {
 757                 m->errorOut(e, "Maligner", "computePercentID");
 758                 exit(1);
 759         }
 760 }
 761 //***************************************************************************************************************