5 * Created by westcott on 5/7/10.
6 * Copyright 2010 Schloss Lab. All rights reserved.
10 #include "makegroupcommand.h"
12 //**********************************************************************************************************************
14 MakeGroupCommand::MakeGroupCommand(string option) {
19 //allow user to run help
20 if(option == "help") { help(); abort = true; }
24 //valid paramters for this command
25 string AlignArray[] = {"fasta","groups","outputdir","inputdir"};
26 vector<string> myArray (AlignArray, AlignArray+(sizeof(AlignArray)/sizeof(string)));
28 OptionParser parser(option);
29 map<string, string> parameters = parser.getParameters();
31 ValidParameters validParameter;
32 map<string, string>::iterator it;
34 //check to make sure all parameters are valid for command
35 for (it = parameters.begin(); it != parameters.end(); it++) {
36 if (validParameter.isValidParameter(it->first, myArray, it->second) != true) { abort = true; }
39 //if the user changes the output directory command factory will send this info to us in the output parameter
40 outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){ outputDir = ""; }
43 //if the user changes the input directory command factory will send this info to us in the output parameter
44 string inputDir = validParameter.validFile(parameters, "inputdir", false);
45 if (inputDir == "not found"){ inputDir = ""; }
48 fastaFileName = validParameter.validFile(parameters, "fasta", false);
49 if (fastaFileName == "not found") { m->mothurOut("fasta is a required parameter for the make.group command."); m->mothurOutEndLine(); abort = true; }
51 splitAtDash(fastaFileName, fastaFileNames);
53 //go through files and make sure they are good, if not, then disregard them
54 for (int i = 0; i < fastaFileNames.size(); i++) {
56 string path = hasPath(fastaFileNames[i]);
57 //if the user has not given a path then, add inputdir. else leave path alone.
58 if (path == "") { fastaFileNames[i] = inputDir + fastaFileNames[i]; }
64 ableToOpen = openInputFile(fastaFileNames[i], in);
67 if (ableToOpen == 1) {
68 m->mothurOut(fastaFileNames[i] + " will be disregarded."); m->mothurOutEndLine();
69 //erase from file list
70 fastaFileNames.erase(fastaFileNames.begin()+i);
76 //make sure there is at least one valid file left
77 if (fastaFileNames.size() == 0) { m->mothurOut("no valid files."); m->mothurOutEndLine(); abort = true; }
84 m->errorOut(e, "MakeGroupCommand", "MakeGroupCommand");
89 //**********************************************************************************************************************
91 MakeGroupCommand::~MakeGroupCommand(){ }
93 //**********************************************************************************************************************
95 void MakeGroupCommand::help(){
97 m->mothurOut("The align.seqs command reads a file containing sequences and creates an alignment file and a report file.\n");
98 m->mothurOut("The align.seqs command parameters are template, candidate, search, ksize, align, match, mismatch, gapopen and gapextend.\n");
99 m->mothurOut("The template and candidate parameters are required. You may enter multiple fasta files by separating their names with dashes. ie. fasta=abrecovery.fasta-amzon.fasta \n");
100 m->mothurOut("The search parameter allows you to specify the method to find most similar template. Your options are: suffix, kmer and blast. The default is kmer.\n");
101 m->mothurOut("The align parameter allows you to specify the alignment method to use. Your options are: gotoh, needleman, blast and noalign. The default is needleman.\n");
102 m->mothurOut("The ksize parameter allows you to specify the kmer size for finding most similar template to candidate. The default is 8.\n");
103 m->mothurOut("The match parameter allows you to specify the bonus for having the same base. The default is 1.0.\n");
104 m->mothurOut("The mistmatch parameter allows you to specify the penalty for having different bases. The default is -1.0.\n");
105 m->mothurOut("The gapopen parameter allows you to specify the penalty for opening a gap in an alignment. The default is -2.0.\n");
106 m->mothurOut("The gapextend parameter allows you to specify the penalty for extending a gap in an alignment. The default is -1.0.\n");
107 m->mothurOut("The flip parameter is used to specify whether or not you want mothur to try the reverse complement if a sequence falls below the threshold. The default is false.\n");
108 m->mothurOut("The threshold is used to specify a cutoff at which an alignment is deemed 'bad' and the reverse complement may be tried. The default threshold is 0.50, meaning 50% of the bases are removed in the alignment.\n");
109 m->mothurOut("If the flip parameter is set to true the reverse complement of the sequence is aligned and the better alignment is reported.\n");
110 m->mothurOut("The default for the threshold parameter is 0.50, meaning at least 50% of the bases must remain or the sequence is reported as potentially reversed.\n");
111 m->mothurOut("The align.seqs command should be in the following format: \n");
112 m->mothurOut("align.seqs(template=yourTemplateFile, candidate=yourCandidateFile, align=yourAlignmentMethod, search=yourSearchmethod, ksize=yourKmerSize, match=yourMatchBonus, mismatch=yourMismatchpenalty, gapopen=yourGapopenPenalty, gapextend=yourGapExtendPenalty) \n");
113 m->mothurOut("Example align.seqs(candidate=candidate.fasta, template=core.filtered, align=kmer, search=gotoh, ksize=8, match=2.0, mismatch=3.0, gapopen=-2.0, gapextend=-1.0)\n");
114 m->mothurOut("Note: No spaces between parameter labels (i.e. candidate), '=' and parameters (i.e.yourFastaFile).\n\n");
116 catch(exception& e) {
117 m->errorOut(e, "MakeGroupCommand", "help");
123 //**********************************************************************************************************************
125 int MakeGroupCommand::execute(){
127 if (abort == true) { return 0; }
130 m->mothurOutEndLine();
131 m->mothurOut("Output File Names: "); m->mothurOutEndLine();
132 //for (int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i]); m->mothurOutEndLine(); }
133 m->mothurOutEndLine();
137 catch(exception& e) {
138 m->errorOut(e, "MakeGroupCommand", "execute");