]> git.donarmstrong.com Git - mothur.git/blob - getseqscommand.cpp
made dups=T default in get.seqs
[mothur.git] / getseqscommand.cpp
1 /*
2  *  getseqscommand.cpp
3  *  Mothur
4  *
5  *  Created by Sarah Westcott on 7/8/09.
6  *  Copyright 2009 Schloss Lab UMASS Amherst. All rights reserved.
7  *
8  */
9
10 #include "getseqscommand.h"
11 #include "sequence.hpp"
12 #include "listvector.hpp"
13
14 //**********************************************************************************************************************
15 vector<string> GetSeqsCommand::getValidParameters(){    
16         try {
17                 string Array[] =  {"fasta","name", "group", "qfile","alignreport", "accnos", "accnos2","dups", "list","taxonomy","outputdir","inputdir"};
18                 vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
19                 return myArray;
20         }
21         catch(exception& e) {
22                 m->errorOut(e, "GetSeqsCommand", "getValidParameters");
23                 exit(1);
24         }
25 }
26 //**********************************************************************************************************************
27 GetSeqsCommand::GetSeqsCommand(){       
28         try {
29                 abort = true; calledHelp = true; 
30                 vector<string> tempOutNames;
31                 outputTypes["fasta"] = tempOutNames;
32                 outputTypes["taxonomy"] = tempOutNames;
33                 outputTypes["name"] = tempOutNames;
34                 outputTypes["group"] = tempOutNames;
35                 outputTypes["alignreport"] = tempOutNames;
36                 outputTypes["list"] = tempOutNames;
37                 outputTypes["qfile"] = tempOutNames;
38                 outputTypes["accnosreport"] = tempOutNames;
39         }
40         catch(exception& e) {
41                 m->errorOut(e, "GetSeqsCommand", "GetSeqsCommand");
42                 exit(1);
43         }
44 }
45 //**********************************************************************************************************************
46 vector<string> GetSeqsCommand::getRequiredParameters(){ 
47         try {
48                 string Array[] =  {"accnos"};
49                 vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
50                 return myArray;
51         }
52         catch(exception& e) {
53                 m->errorOut(e, "GetSeqsCommand", "getRequiredParameters");
54                 exit(1);
55         }
56 }
57 //**********************************************************************************************************************
58 vector<string> GetSeqsCommand::getRequiredFiles(){      
59         try {
60                 vector<string> myArray;
61                 return myArray;
62         }
63         catch(exception& e) {
64                 m->errorOut(e, "GetSeqsCommand", "getRequiredFiles");
65                 exit(1);
66         }
67 }
68 //**********************************************************************************************************************
69 GetSeqsCommand::GetSeqsCommand(string option)  {
70         try {
71                 abort = false; calledHelp = false;   
72                                 
73                 //allow user to run help
74                 if(option == "help") { help(); abort = true; calledHelp = true; }
75                 
76                 else {
77                         //valid paramters for this command
78                         string Array[] =  {"fasta","name", "group", "alignreport", "qfile", "accnos", "accnos2","dups", "list","taxonomy","outputdir","inputdir"};
79                         vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
80                         
81                         OptionParser parser(option);
82                         map<string,string> parameters = parser.getParameters();
83                         
84                         ValidParameters validParameter;
85                         map<string,string>::iterator it;
86                         
87                         //check to make sure all parameters are valid for command
88                         for (it = parameters.begin(); it != parameters.end(); it++) { 
89                                 if (validParameter.isValidParameter(it->first, myArray, it->second) != true) {  abort = true;  }
90                         }
91                         
92                         //initialize outputTypes
93                         vector<string> tempOutNames;
94                         outputTypes["fasta"] = tempOutNames;
95                         outputTypes["taxonomy"] = tempOutNames;
96                         outputTypes["name"] = tempOutNames;
97                         outputTypes["group"] = tempOutNames;
98                         outputTypes["alignreport"] = tempOutNames;
99                         outputTypes["list"] = tempOutNames;
100                         outputTypes["qfile"] = tempOutNames;
101                         outputTypes["accnosreport"] = tempOutNames;
102                         
103                         //if the user changes the output directory command factory will send this info to us in the output parameter 
104                         outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){  outputDir = "";         }
105                         
106                         //if the user changes the input directory command factory will send this info to us in the output parameter 
107                         string inputDir = validParameter.validFile(parameters, "inputdir", false);              
108                         if (inputDir == "not found"){   inputDir = "";          }
109                         else {
110                                 string path;
111                                 it = parameters.find("alignreport");
112                                 //user has given a template file
113                                 if(it != parameters.end()){ 
114                                         path = m->hasPath(it->second);
115                                         //if the user has not given a path then, add inputdir. else leave path alone.
116                                         if (path == "") {       parameters["alignreport"] = inputDir + it->second;              }
117                                 }
118                                 
119                                 it = parameters.find("fasta");
120                                 //user has given a template file
121                                 if(it != parameters.end()){ 
122                                         path = m->hasPath(it->second);
123                                         //if the user has not given a path then, add inputdir. else leave path alone.
124                                         if (path == "") {       parameters["fasta"] = inputDir + it->second;            }
125                                 }
126                                 
127                                 it = parameters.find("accnos");
128                                 //user has given a template file
129                                 if(it != parameters.end()){ 
130                                         path = m->hasPath(it->second);
131                                         //if the user has not given a path then, add inputdir. else leave path alone.
132                                         if (path == "") {       parameters["accnos"] = inputDir + it->second;           }
133                                 }
134                                 
135                                 it = parameters.find("accnos2");
136                                 //user has given a template file
137                                 if(it != parameters.end()){ 
138                                         path = m->hasPath(it->second);
139                                         //if the user has not given a path then, add inputdir. else leave path alone.
140                                         if (path == "") {       parameters["accnos2"] = inputDir + it->second;          }
141                                 }
142                                 
143                                 it = parameters.find("list");
144                                 //user has given a template file
145                                 if(it != parameters.end()){ 
146                                         path = m->hasPath(it->second);
147                                         //if the user has not given a path then, add inputdir. else leave path alone.
148                                         if (path == "") {       parameters["list"] = inputDir + it->second;             }
149                                 }
150                                 
151                                 it = parameters.find("name");
152                                 //user has given a template file
153                                 if(it != parameters.end()){ 
154                                         path = m->hasPath(it->second);
155                                         //if the user has not given a path then, add inputdir. else leave path alone.
156                                         if (path == "") {       parameters["name"] = inputDir + it->second;             }
157                                 }
158                                 
159                                 it = parameters.find("group");
160                                 //user has given a template file
161                                 if(it != parameters.end()){ 
162                                         path = m->hasPath(it->second);
163                                         //if the user has not given a path then, add inputdir. else leave path alone.
164                                         if (path == "") {       parameters["group"] = inputDir + it->second;            }
165                                 }
166                                 
167                                 it = parameters.find("taxonomy");
168                                 //user has given a template file
169                                 if(it != parameters.end()){ 
170                                         path = m->hasPath(it->second);
171                                         //if the user has not given a path then, add inputdir. else leave path alone.
172                                         if (path == "") {       parameters["taxonomy"] = inputDir + it->second;         }
173                                 }
174                                 
175                                 it = parameters.find("qfile");
176                                 //user has given a template file
177                                 if(it != parameters.end()){ 
178                                         path = m->hasPath(it->second);
179                                         //if the user has not given a path then, add inputdir. else leave path alone.
180                                         if (path == "") {       parameters["qfile"] = inputDir + it->second;            }
181                                 }
182                         }
183
184                         
185                         //check for required parameters
186                         accnosfile = validParameter.validFile(parameters, "accnos", true);
187                         if (accnosfile == "not open") { abort = true; }
188                         else if (accnosfile == "not found") {  accnosfile = "";  m->mothurOut("You must provide an accnos file."); m->mothurOutEndLine(); abort = true; }       
189                         
190                         if (accnosfile2 == "not found") { accnosfile2 = ""; }
191                         
192                         fastafile = validParameter.validFile(parameters, "fasta", true);
193                         if (fastafile == "not open") { abort = true; }
194                         else if (fastafile == "not found") {  fastafile = "";  }        
195                         
196                         namefile = validParameter.validFile(parameters, "name", true);
197                         if (namefile == "not open") { abort = true; }
198                         else if (namefile == "not found") {  namefile = "";  }  
199                         
200                         groupfile = validParameter.validFile(parameters, "group", true);
201                         if (groupfile == "not open") { abort = true; }
202                         else if (groupfile == "not found") {  groupfile = "";  }        
203                         
204                         alignfile = validParameter.validFile(parameters, "alignreport", true);
205                         if (alignfile == "not open") { abort = true; }
206                         else if (alignfile == "not found") {  alignfile = "";  }
207                         
208                         listfile = validParameter.validFile(parameters, "list", true);
209                         if (listfile == "not open") { abort = true; }
210                         else if (listfile == "not found") {  listfile = "";  }
211                         
212                         taxfile = validParameter.validFile(parameters, "taxonomy", true);
213                         if (taxfile == "not open") { abort = true; }
214                         else if (taxfile == "not found") {  taxfile = "";  }
215                         
216                         qualfile = validParameter.validFile(parameters, "qfile", true);
217                         if (qualfile == "not open") { abort = true; }
218                         else if (qualfile == "not found") {  qualfile = "";  }
219                         
220                         string usedDups = "true";
221                         string temp = validParameter.validFile(parameters, "dups", false);      if (temp == "not found") { temp = "true"; usedDups = ""; }
222                         dups = m->isTrue(temp);
223                         
224                         if ((fastafile == "") && (namefile == "") && (groupfile == "") && (alignfile == "") && (listfile == "") && (taxfile == "") && (qualfile == "") && (accnosfile2 == ""))  { m->mothurOut("You must provide one of the following: fasta, name, group, alignreport, taxonomy, quality or listfile."); m->mothurOutEndLine(); abort = true; }
225                 
226                         if ((usedDups != "") && (namefile == "")) {  m->mothurOut("You may only use dups with the name option."); m->mothurOutEndLine();  abort = true; }                       
227
228                 }
229
230         }
231         catch(exception& e) {
232                 m->errorOut(e, "GetSeqsCommand", "GetSeqsCommand");
233                 exit(1);
234         }
235 }
236 //**********************************************************************************************************************
237
238 void GetSeqsCommand::help(){
239         try {
240                 m->mothurOut("The get.seqs command reads an .accnos file and any of the following file types: fasta, name, group, list, taxonomy, quality or alignreport file.\n");
241                 m->mothurOut("It outputs a file containing only the sequences in the .accnos file.\n");
242                 m->mothurOut("The get.seqs command parameters are accnos, fasta, name, group, list, taxonomy, qfile, alignreport and dups.  You must provide accnos and at least one of the other parameters.\n");
243                 m->mothurOut("The dups parameter allows you to add the entire line from a name file if you add any name from the line. default=false. \n");
244                 m->mothurOut("The get.seqs command should be in the following format: get.seqs(accnos=yourAccnos, fasta=yourFasta).\n");
245                 m->mothurOut("Example get.seqs(accnos=amazon.accnos, fasta=amazon.fasta).\n");
246                 m->mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFasta).\n\n");
247         }
248         catch(exception& e) {
249                 m->errorOut(e, "GetSeqsCommand", "help");
250                 exit(1);
251         }
252 }
253
254 //**********************************************************************************************************************
255
256 int GetSeqsCommand::execute(){
257         try {
258                 
259                 if (abort == true) { if (calledHelp) { return 0; }  return 2;   }
260                 
261                 //get names you want to keep
262                 readAccnos();
263                 
264                 if (m->control_pressed) { return 0; }
265                 
266                 //read through the correct file and output lines you want to keep
267                 if (namefile != "")                     {               readName();                     }
268                 if (fastafile != "")            {               readFasta();            }
269                 if (groupfile != "")            {               readGroup();            }
270                 if (alignfile != "")            {               readAlign();            }
271                 if (listfile != "")                     {               readList();                     }
272                 if (taxfile != "")                      {               readTax();                      }
273                 if (qualfile != "")                     {               readQual();                     }
274                 if (accnosfile2 != "")          {               compareAccnos();        }
275                 
276                 if (m->control_pressed) { outputTypes.clear(); for (int i = 0; i < outputNames.size(); i++) {   remove(outputNames[i].c_str());  } return 0; }
277                 
278                 m->mothurOut("Selected " + toString(names.size()) + " sequences."); m->mothurOutEndLine();
279                 
280                 if (outputNames.size() != 0) {
281                         m->mothurOutEndLine();
282                         m->mothurOut("Output File Names: "); m->mothurOutEndLine();
283                         for (int i = 0; i < outputNames.size(); i++) {  m->mothurOut(outputNames[i]); m->mothurOutEndLine();    }
284                         m->mothurOutEndLine();
285                         
286                         //set fasta file as new current fastafile
287                         string current = "";
288                         itTypes = outputTypes.find("fasta");
289                         if (itTypes != outputTypes.end()) {
290                                 if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setFastaFile(current); }
291                         }
292                         
293                         itTypes = outputTypes.find("name");
294                         if (itTypes != outputTypes.end()) {
295                                 if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setNameFile(current); }
296                         }
297                         
298                         itTypes = outputTypes.find("group");
299                         if (itTypes != outputTypes.end()) {
300                                 if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setGroupFile(current); }
301                         }
302                         
303                         itTypes = outputTypes.find("list");
304                         if (itTypes != outputTypes.end()) {
305                                 if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setListFile(current); }
306                         }
307                         
308                         itTypes = outputTypes.find("taxonomy");
309                         if (itTypes != outputTypes.end()) {
310                                 if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setTaxonomyFile(current); }
311                         }
312                         
313                         itTypes = outputTypes.find("qfile");
314                         if (itTypes != outputTypes.end()) {
315                                 if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setQualFile(current); }
316                         }
317                         
318                 }
319                 
320                 return 0;               
321         }
322
323         catch(exception& e) {
324                 m->errorOut(e, "GetSeqsCommand", "execute");
325                 exit(1);
326         }
327 }
328
329 //**********************************************************************************************************************
330 int GetSeqsCommand::readFasta(){
331         try {
332                 string thisOutputDir = outputDir;
333                 if (outputDir == "") {  thisOutputDir += m->hasPath(fastafile);  }
334                 string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(fastafile)) + "pick" +  m->getExtension(fastafile);
335                 ofstream out;
336                 m->openOutputFile(outputFileName, out);
337                 
338                 
339                 ifstream in;
340                 m->openInputFile(fastafile, in);
341                 string name;
342                 
343                 bool wroteSomething = false;
344                 
345                 while(!in.eof()){
346                 
347                         if (m->control_pressed) { in.close(); out.close(); remove(outputFileName.c_str());  return 0; }
348                         
349                         Sequence currSeq(in);
350                         name = currSeq.getName();
351                         
352                         if (name != "") {
353                                 //if this name is in the accnos file
354                                 if (names.count(name) != 0) {
355                                         wroteSomething = true;
356                                         
357                                         currSeq.printSequence(out);
358                                 }
359                         }
360                         m->gobble(in);
361                 }
362                 in.close();     
363                 out.close();
364                 
365                 
366                 if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine();  }
367                 outputNames.push_back(outputFileName);  outputTypes["fasta"].push_back(outputFileName); 
368                 
369                 return 0;
370
371         }
372         catch(exception& e) {
373                 m->errorOut(e, "GetSeqsCommand", "readFasta");
374                 exit(1);
375         }
376 }
377 //**********************************************************************************************************************
378 int GetSeqsCommand::readQual(){
379         try {
380                 string thisOutputDir = outputDir;
381                 if (outputDir == "") {  thisOutputDir += m->hasPath(qualfile);  }
382                 string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(qualfile)) + "pick" +  m->getExtension(qualfile);
383                 ofstream out;
384                 m->openOutputFile(outputFileName, out);
385                 
386                 
387                 ifstream in;
388                 m->openInputFile(qualfile, in);
389                 string name;
390                 
391                 bool wroteSomething = false;
392                 
393                 
394                 while(!in.eof()){       
395                         string saveName = "";
396                         string name = "";
397                         string scores = "";
398                         
399                         in >> name; 
400                                 
401                         if (name.length() != 0) { 
402                                 saveName = name.substr(1);
403                                 while (!in.eof())       {       
404                                         char c = in.get(); 
405                                         if (c == 10 || c == 13){        break;  }
406                                         else { name += c; }     
407                                 } 
408                                 m->gobble(in);
409                         }
410                         
411                         while(in){
412                                 char letter= in.get();
413                                 if(letter == '>'){      in.putback(letter);     break;  }
414                                 else{ scores += letter; }
415                         }
416                         
417                         m->gobble(in);
418                         
419                         if (names.count(saveName) != 0) {
420                                 wroteSomething = true;
421                                                 
422                                 out << name << endl << scores;
423                         }
424                         
425                         m->gobble(in);
426                 }
427                 in.close();
428                 out.close();
429                 
430                 
431                 if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine();  }
432                 outputNames.push_back(outputFileName);  outputTypes["qfile"].push_back(outputFileName); 
433                 
434                 return 0;
435                 
436         }
437         catch(exception& e) {
438                 m->errorOut(e, "GetSeqsCommand", "readQual");
439                 exit(1);
440         }
441 }
442 //**********************************************************************************************************************
443 int GetSeqsCommand::readList(){
444         try {
445                 string thisOutputDir = outputDir;
446                 if (outputDir == "") {  thisOutputDir += m->hasPath(listfile);  }
447                 string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(listfile)) + "pick" +  m->getExtension(listfile);
448                 ofstream out;
449                 m->openOutputFile(outputFileName, out);
450                 
451                 ifstream in;
452                 m->openInputFile(listfile, in);
453                 
454                 bool wroteSomething = false;
455                 
456                 while(!in.eof()){
457                         
458                         if (m->control_pressed) { in.close(); out.close(); remove(outputFileName.c_str());  return 0; }
459
460                         //read in list vector
461                         ListVector list(in);
462                         
463                         //make a new list vector
464                         ListVector newList;
465                         newList.setLabel(list.getLabel());
466                         
467                         //for each bin
468                         for (int i = 0; i < list.getNumBins(); i++) {
469                         
470                                 //parse out names that are in accnos file
471                                 string binnames = list.get(i);
472                                 
473                                 string newNames = "";
474                                 while (binnames.find_first_of(',') != -1) { 
475                                         string name = binnames.substr(0,binnames.find_first_of(','));
476                                         binnames = binnames.substr(binnames.find_first_of(',')+1, binnames.length());
477                                         
478                                         //if that name is in the .accnos file, add it
479                                         if (names.count(name) != 0) {  newNames += name + ",";  }
480                                 }
481                         
482                                 //get last name
483                                 if (names.count(binnames) != 0) {  newNames += binnames + ",";  }
484
485                                 //if there are names in this bin add to new list
486                                 if (newNames != "") { 
487                                         newNames = newNames.substr(0, newNames.length()-1); //rip off extra comma
488                                         newList.push_back(newNames);    
489                                 }
490                         }
491                                 
492                         //print new listvector
493                         if (newList.getNumBins() != 0) {
494                                 wroteSomething = true;
495                                 newList.print(out);
496                         }
497                         
498                         m->gobble(in);
499                 }
500                 in.close();     
501                 out.close();
502                 
503                 if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine();  }
504                 outputNames.push_back(outputFileName); outputTypes["list"].push_back(outputFileName);
505                 
506                 return 0;
507
508         }
509         catch(exception& e) {
510                 m->errorOut(e, "GetSeqsCommand", "readList");
511                 exit(1);
512         }
513 }
514 //**********************************************************************************************************************
515 int GetSeqsCommand::readName(){
516         try {
517                 string thisOutputDir = outputDir;
518                 if (outputDir == "") {  thisOutputDir += m->hasPath(namefile);  }
519                 string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(namefile)) + "pick" +  m->getExtension(namefile);
520                 ofstream out;
521                 m->openOutputFile(outputFileName, out);
522                 
523
524                 ifstream in;
525                 m->openInputFile(namefile, in);
526                 string name, firstCol, secondCol;
527                 
528                 bool wroteSomething = false;
529                 
530                 
531                 while(!in.eof()){
532                 
533                         if (m->control_pressed) { in.close(); out.close(); remove(outputFileName.c_str());  return 0; }
534
535                         in >> firstCol;                         
536                         in >> secondCol;
537                         
538                         string hold = "";
539                         if (dups) { hold = secondCol; }
540                         
541                         vector<string> parsedNames;
542                         //parse second column saving each name
543                         while (secondCol.find_first_of(',') != -1) { 
544                                 name = secondCol.substr(0,secondCol.find_first_of(','));
545                                 secondCol = secondCol.substr(secondCol.find_first_of(',')+1, secondCol.length());
546                                 parsedNames.push_back(name);
547                         }
548                         
549                         //get name after last ,
550                         parsedNames.push_back(secondCol);
551                         
552                         vector<string> validSecond;
553                         for (int i = 0; i < parsedNames.size(); i++) {
554                                 if (names.count(parsedNames[i]) != 0) {
555                                         validSecond.push_back(parsedNames[i]);
556                                 }
557                         }
558
559                         if ((dups) && (validSecond.size() != 0)) { //dups = true and we want to add someone, then add everyone
560                                 for (int i = 0; i < parsedNames.size(); i++) {  names.insert(parsedNames[i]);  }
561                                 out << firstCol << '\t' << hold << endl;
562                                 wroteSomething = true;
563                         }else {
564                                 //if the name in the first column is in the set then print it and any other names in second column also in set
565                                 if (names.count(firstCol) != 0) {
566                                 
567                                         wroteSomething = true;
568                                         
569                                         out << firstCol << '\t';
570                                         
571                                         //you know you have at least one valid second since first column is valid
572                                         for (int i = 0; i < validSecond.size()-1; i++) {  out << validSecond[i] << ',';  }
573                                         out << validSecond[validSecond.size()-1] << endl;
574                                         
575                                 
576                                 //make first name in set you come to first column and then add the remaining names to second column
577                                 }else {
578                                         //you want part of this row
579                                         if (validSecond.size() != 0) {
580                                         
581                                                 wroteSomething = true;
582                                                 
583                                                 out << validSecond[0] << '\t';
584                                         
585                                                 //you know you have at least one valid second since first column is valid
586                                                 for (int i = 0; i < validSecond.size()-1; i++) {  out << validSecond[i] << ',';  }
587                                                 out << validSecond[validSecond.size()-1] << endl;
588                                         }
589                                 }
590                         }
591                         m->gobble(in);
592                 }
593                 in.close();
594                 out.close();
595                 
596                 if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine();  }
597                 outputNames.push_back(outputFileName); outputTypes["name"].push_back(outputFileName);
598                 
599                 return 0;
600                 
601         }
602         catch(exception& e) {
603                 m->errorOut(e, "GetSeqsCommand", "readName");
604                 exit(1);
605         }
606 }
607
608 //**********************************************************************************************************************
609 int GetSeqsCommand::readGroup(){
610         try {
611                 string thisOutputDir = outputDir;
612                 if (outputDir == "") {  thisOutputDir += m->hasPath(groupfile);  }
613                 string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(groupfile)) + "pick" + m->getExtension(groupfile);
614                 ofstream out;
615                 m->openOutputFile(outputFileName, out);
616                 
617
618                 ifstream in;
619                 m->openInputFile(groupfile, in);
620                 string name, group;
621                 
622                 bool wroteSomething = false;
623                 
624                 while(!in.eof()){
625
626                         if (m->control_pressed) { in.close(); out.close(); remove(outputFileName.c_str());  return 0; }
627
628
629                         in >> name;                             //read from first column
630                         in >> group;                    //read from second column
631                         
632                         //if this name is in the accnos file
633                         if (names.count(name) != 0) {
634                                 wroteSomething = true;
635                                 
636                                 out << name << '\t' << group << endl;
637                         }
638                                         
639                         m->gobble(in);
640                 }
641                 in.close();
642                 out.close();
643                 
644                 if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine();  }
645                 outputNames.push_back(outputFileName);  outputTypes["group"].push_back(outputFileName);
646                 
647                 return 0;
648
649         }
650         catch(exception& e) {
651                 m->errorOut(e, "GetSeqsCommand", "readGroup");
652                 exit(1);
653         }
654 }
655 //**********************************************************************************************************************
656 int GetSeqsCommand::readTax(){
657         try {
658                 string thisOutputDir = outputDir;
659                 if (outputDir == "") {  thisOutputDir += m->hasPath(taxfile);  }
660                 string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(taxfile)) + "pick" + m->getExtension(taxfile);
661                 ofstream out;
662                 m->openOutputFile(outputFileName, out);
663                 
664                 ifstream in;
665                 m->openInputFile(taxfile, in);
666                 string name, tax;
667                 
668                 bool wroteSomething = false;
669                 
670                 while(!in.eof()){
671
672                         if (m->control_pressed) { in.close(); out.close(); remove(outputFileName.c_str());  return 0; }
673
674                         in >> name;                             //read from first column
675                         in >> tax;                      //read from second column
676                         
677                         //if this name is in the accnos file
678                         if (names.count(name) != 0) {
679                                 wroteSomething = true;
680                                 
681                                 out << name << '\t' << tax << endl;
682                         }
683                                         
684                         m->gobble(in);
685                 }
686                 in.close();
687                 out.close();
688                 
689                 if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine();  }
690                 outputNames.push_back(outputFileName);  outputTypes["taxonomy"].push_back(outputFileName);
691                         
692                 return 0;
693
694         }
695         catch(exception& e) {
696                 m->errorOut(e, "GetSeqsCommand", "readTax");
697                 exit(1);
698         }
699 }
700 //**********************************************************************************************************************
701 //alignreport file has a column header line then all other lines contain 16 columns.  we just want the first column since that contains the name
702 int GetSeqsCommand::readAlign(){
703         try {
704                 string thisOutputDir = outputDir;
705                 if (outputDir == "") {  thisOutputDir += m->hasPath(alignfile);  }
706                 string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(alignfile)) + "pick.align.report";
707                 ofstream out;
708                 m->openOutputFile(outputFileName, out);
709                 
710
711                 ifstream in;
712                 m->openInputFile(alignfile, in);
713                 string name, junk;
714                 
715                 bool wroteSomething = false;
716                 
717                 //read column headers
718                 for (int i = 0; i < 16; i++) {  
719                         if (!in.eof())  {       in >> junk;      out << junk << '\t';   }
720                         else                    {       break;                  }
721                 }
722                 out << endl;
723                 
724                 while(!in.eof()){
725                 
726                         if (m->control_pressed) { in.close(); out.close(); remove(outputFileName.c_str());  return 0; }
727
728
729                         in >> name;                             //read from first column
730                         
731                         //if this name is in the accnos file
732                         if (names.count(name) != 0) {
733                                 wroteSomething = true;
734                                 
735                                 out << name << '\t';
736                                 
737                                 //read rest
738                                 for (int i = 0; i < 15; i++) {  
739                                         if (!in.eof())  {       in >> junk;      out << junk << '\t';   }
740                                         else                    {       break;                  }
741                                 }
742                                 out << endl;
743                                 
744                         }else {//still read just don't do anything with it
745                                 //read rest
746                                 for (int i = 0; i < 15; i++) {  
747                                         if (!in.eof())  {       in >> junk;             }
748                                         else                    {       break;                  }
749                                 }
750                         }
751                         
752                         m->gobble(in);
753                 }
754                 in.close();
755                 out.close();
756                 
757                 if (wroteSomething == false) { m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine();  }
758                 outputNames.push_back(outputFileName);  outputTypes["alignreport"].push_back(outputFileName);
759                 
760                 return 0;
761                 
762         }
763         catch(exception& e) {
764                 m->errorOut(e, "GetSeqsCommand", "readAlign");
765                 exit(1);
766         }
767 }
768 //**********************************************************************************************************************
769
770 int GetSeqsCommand::readAccnos(){
771         try {
772                 
773                 ifstream in;
774                 m->openInputFile(accnosfile, in);
775                 string name;
776                 
777                 while(!in.eof()){
778                         in >> name;
779                                                 
780                         names.insert(name);
781                         
782                         m->gobble(in);
783                 }
784                 in.close();     
785                 
786                 return 0;
787
788         }
789         catch(exception& e) {
790                 m->errorOut(e, "GetSeqsCommand", "readAccnos");
791                 exit(1);
792         }
793 }
794 //**********************************************************************************************************************
795
796 int GetSeqsCommand::compareAccnos(){
797         try {
798                 
799                 string thisOutputDir = outputDir;
800                 if (outputDir == "") {  thisOutputDir += m->hasPath(accnosfile);  }
801                 string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(accnosfile)) + "accnos.report";
802                 ofstream out;
803                 m->openOutputFile(outputFileName, out);
804                 
805                 ifstream in;
806                 m->openInputFile(accnosfile2, in);
807                 string name;
808                 
809                 set<string> namesAccnos2;
810                 set<string> namesDups;
811                 set<string> namesAccnos = names;
812                 
813                 map<string, int> nameCount;
814                 
815                 if (namefile != "") {
816                         ifstream inName;
817                         m->openInputFile(namefile, inName);
818                         
819                         
820                         while(!inName.eof()){
821                                 
822                                 if (m->control_pressed) { inName.close(); return 0; }
823                                 
824                                 string thisname, repnames;
825                                 
826                                 inName >> thisname;             m->gobble(inName);              //read from first column
827                                 inName >> repnames;                     //read from second column
828                                 
829                                 int num = m->getNumNames(repnames);
830                                 nameCount[thisname] = num;
831                                 
832                                 m->gobble(inName);
833                         }
834                         inName.close(); 
835                 }
836                 
837                 while(!in.eof()){
838                         in >> name;
839                         
840                         if (namesAccnos.count(name) == 0){ //name unique to accnos2
841                                 namesAccnos2.insert(name);
842                         }else { //you are in both so erase
843                                 namesAccnos.erase(name);
844                                 namesDups.insert(name);
845                         }
846                         
847                         m->gobble(in);
848                 }
849                 in.close();     
850                 
851                 out << "Names in both files : " + toString(namesDups.size()) << endl;
852                 m->mothurOut("Names in both files : " + toString(namesDups.size())); m->mothurOutEndLine();
853                 
854                 for (set<string>::iterator it = namesDups.begin(); it != namesDups.end(); it++) {
855                         out << (*it);
856                         if (namefile != "") { out << '\t' << nameCount[(*it)]; }
857                         out << endl;
858                 }
859                 
860                 out << "Names unique to " + accnosfile + " : " + toString(namesAccnos.size()) << endl;
861                 m->mothurOut("Names unique to " + accnosfile + " : " + toString(namesAccnos.size())); m->mothurOutEndLine();
862                 
863                 for (set<string>::iterator it = namesAccnos.begin(); it != namesAccnos.end(); it++) {
864                         out << (*it);
865                         if (namefile != "") { out << '\t' << nameCount[(*it)]; }
866                         out << endl;
867                 }
868                 
869                 out << "Names unique to " + accnosfile2 + " : " + toString(namesAccnos2.size()) << endl;
870                 m->mothurOut("Names unique to " + accnosfile2 + " : " + toString(namesAccnos2.size())); m->mothurOutEndLine();
871                 
872                 for (set<string>::iterator it = namesAccnos2.begin(); it != namesAccnos2.end(); it++) {
873                         out << (*it);
874                         if (namefile != "") { out << '\t' << nameCount[(*it)]; }
875                         out << endl;
876                 }
877
878                 out.close(); 
879                 
880                 outputNames.push_back(outputFileName);  outputTypes["accnosreport"].push_back(outputFileName);
881                 
882                 return 0;
883                 
884         }
885         catch(exception& e) {
886                 m->errorOut(e, "GetSeqsCommand", "readAccnos");
887                 exit(1);
888         }
889 }
890
891
892 //**********************************************************************************************************************
893