classifyseqscommand.cpp

   1 /*
   2  *  classifyseqscommand.cpp
   3  *  Mothur
   4  *
   5  *  Created by westcott on 11/2/09.
   6  *  Copyright 2009 Schloss Lab. All rights reserved.
   7  *
   8  */
   9
  10 #include "classifyseqscommand.h"
  11 #include "sequence.hpp"
  12 #include "bayesian.h"
  13 #include "phylotree.h"
  14 #include "phylosummary.h"
  15 #include "knn.h"
  16
  17
  18 //**********************************************************************************************************************
  19 vector<string> ClassifySeqsCommand::getValidParameters(){
  20         try {
  21                 string AlignArray[] =  {"template","fasta","name","group","search","ksize","method","processors","taxonomy","match","mismatch","gapopen","gapextend","numwanted","cutoff","probs","iters", "outputdir","inputdir"};
  22                 vector<string> myArray (AlignArray, AlignArray+(sizeof(AlignArray)/sizeof(string)));
  23                 return myArray;
  24         }
  25         catch(exception& e) {
  26                 m->errorOut(e, "ClassifySeqsCommand", "getValidParameters");
  27                 exit(1);
  28         }
  29 }
  30 //**********************************************************************************************************************
  31 ClassifySeqsCommand::ClassifySeqsCommand(){
  32         try {
  33                 //initialize outputTypes
  34                 vector<string> tempOutNames;
  35                 outputTypes["taxonomy"] = tempOutNames;
  36                 outputTypes["taxsummary"] = tempOutNames;
  37                 outputTypes["matchdist"] = tempOutNames;
  38         }
  39         catch(exception& e) {
  40                 m->errorOut(e, "ClassifySeqsCommand", "ClassifySeqsCommand");
  41                 exit(1);
  42         }
  43 }
  44 //**********************************************************************************************************************
  45 vector<string> ClassifySeqsCommand::getRequiredParameters(){
  46         try {
  47                 string Array[] =  {"fasta","template","taxonomy"};
  48                 vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
  49                 return myArray;
  50         }
  51         catch(exception& e) {
  52                 m->errorOut(e, "ClassifySeqsCommand", "getRequiredParameters");
  53                 exit(1);
  54         }
  55 }
  56 //**********************************************************************************************************************
  57 vector<string> ClassifySeqsCommand::getRequiredFiles(){
  58         try {
  59                 vector<string> myArray;
  60                 return myArray;
  61         }
  62         catch(exception& e) {
  63                 m->errorOut(e, "ClassifySeqsCommand", "getRequiredFiles");
  64                 exit(1);
  65         }
  66 }
  67 //**********************************************************************************************************************
  68 ClassifySeqsCommand::ClassifySeqsCommand(string option)  {
  69         try {
  70                 abort = false;
  71
  72                 //allow user to run help
  73                 if(option == "help") { help(); abort = true; }
  74
  75                 else {
  76
  77                         //valid paramters for this command
  78                         string AlignArray[] =  {"template","fasta","name","group","search","ksize","method","processors","taxonomy","match","mismatch","gapopen","gapextend","numwanted","cutoff","probs","iters", "outputdir","inputdir"};
  79                         vector<string> myArray (AlignArray, AlignArray+(sizeof(AlignArray)/sizeof(string)));
  80
  81                         OptionParser parser(option);
  82                         map<string, string> parameters = parser.getParameters();
  83
  84                         ValidParameters validParameter("classify.seqs");
  85                         map<string, string>::iterator it;
  86
  87                         //check to make sure all parameters are valid for command
  88                         for (it = parameters.begin(); it != parameters.end(); it++) {
  89                                 if (validParameter.isValidParameter(it->first, myArray, it->second) != true) {  abort = true;  }
  90                         }
  91
  92                         //initialize outputTypes
  93                         vector<string> tempOutNames;
  94                         outputTypes["taxonomy"] = tempOutNames;
  95                         outputTypes["taxsummary"] = tempOutNames;
  96                         outputTypes["matchdist"] = tempOutNames;
  97
  98                         //if the user changes the output directory command factory will send this info to us in the output parameter
  99                         outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){  outputDir = "";         }
 100
 101                         //if the user changes the input directory command factory will send this info to us in the output parameter
 102                         string inputDir = validParameter.validFile(parameters, "inputdir", false);
 103                         if (inputDir == "not found"){   inputDir = "";          }
 104                         else {
 105                                 string path;
 106                                 it = parameters.find("template");
 107                                 //user has given a template file
 108                                 if(it != parameters.end()){
 109                                         path = m->hasPath(it->second);
 110                                         //if the user has not given a path then, add inputdir. else leave path alone.
 111                                         if (path == "") {       parameters["template"] = inputDir + it->second;         }
 112                                 }
 113
 114                                 it = parameters.find("taxonomy");
 115                                 //user has given a template file
 116                                 if(it != parameters.end()){
 117                                         path = m->hasPath(it->second);
 118                                         //if the user has not given a path then, add inputdir. else leave path alone.
 119                                         if (path == "") {       parameters["taxonomy"] = inputDir + it->second;         }
 120                                 }
 121
 122                                 it = parameters.find("group");
 123                                 //user has given a template file
 124                                 if(it != parameters.end()){
 125                                         path = m->hasPath(it->second);
 126                                         //if the user has not given a path then, add inputdir. else leave path alone.
 127                                         if (path == "") {       parameters["group"] = inputDir + it->second;            }
 128                                 }
 129                         }
 130
 131                         //check for required parameters
 132                         templateFileName = validParameter.validFile(parameters, "template", true);
 133                         if (templateFileName == "not found") {
 134                                 m->mothurOut("template is a required parameter for the classify.seqs command.");
 135                                 m->mothurOutEndLine();
 136                                 abort = true;
 137                         }
 138                         else if (templateFileName == "not open") { abort = true; }
 139
 140
 141                         fastaFileName = validParameter.validFile(parameters, "fasta", false);
 142                         if (fastaFileName == "not found") { m->mothurOut("fasta is a required parameter for the classify.seqs command."); m->mothurOutEndLine(); abort = true;  }
 143                         else {
 144                                 m->splitAtDash(fastaFileName, fastaFileNames);
 145
 146                                 //go through files and make sure they are good, if not, then disregard them
 147                                 for (int i = 0; i < fastaFileNames.size(); i++) {
 148                                         if (inputDir != "") {
 149                                                 string path = m->hasPath(fastaFileNames[i]);
 150                                                 //if the user has not given a path then, add inputdir. else leave path alone.
 151                                                 if (path == "") {       fastaFileNames[i] = inputDir + fastaFileNames[i];               }
 152                                         }
 153
 154                                         int ableToOpen;
 155
 156                                         ifstream in;
 157                                         ableToOpen = m->openInputFile(fastaFileNames[i], in, "noerror");
 158
 159                                         //if you can't open it, try default location
 160                                         if (ableToOpen == 1) {
 161                                                 if (m->getDefaultPath() != "") { //default path is set
 162                                                         string tryPath = m->getDefaultPath() + m->getSimpleName(fastaFileNames[i]);
 163                                                         m->mothurOut("Unable to open " + fastaFileNames[i] + ". Trying default " + tryPath); m->mothurOutEndLine();
 164                                                         ifstream in2;
 165                                                         ableToOpen = m->openInputFile(tryPath, in2, "noerror");
 166                                                         in2.close();
 167                                                         fastaFileNames[i] = tryPath;
 168                                                 }
 169                                         }
 170
 171                                         if (ableToOpen == 1) {
 172                                                 if (m->getOutputDir() != "") { //default path is set
 173                                                         string tryPath = m->getOutputDir() + m->getSimpleName(fastaFileNames[i]);
 174                                                         m->mothurOut("Unable to open " + fastaFileNames[i] + ". Trying output directory " + tryPath); m->mothurOutEndLine();
 175                                                         ifstream in2;
 176                                                         ableToOpen = m->openInputFile(tryPath, in2, "noerror");
 177                                                         in2.close();
 178                                                         fastaFileNames[i] = tryPath;
 179                                                 }
 180                                         }
 181
 182                                         in.close();
 183
 184                                         if (ableToOpen == 1) {
 185                                                 m->mothurOut("Unable to open " + fastaFileNames[i] + ". It will be disregarded."); m->mothurOutEndLine();
 186                                                 //erase from file list
 187                                                 fastaFileNames.erase(fastaFileNames.begin()+i);
 188                                                 i--;
 189                                         }
 190
 191                                 }
 192
 193                                 //make sure there is at least one valid file left
 194                                 if (fastaFileNames.size() == 0) { m->mothurOut("no valid files."); m->mothurOutEndLine(); abort = true; }
 195                         }
 196
 197
 198                         taxonomyFileName = validParameter.validFile(parameters, "taxonomy", true);
 199                         if (taxonomyFileName == "not found") {
 200                                 m->mothurOut("taxonomy is a required parameter for the classify.seqs command.");
 201                                 m->mothurOutEndLine();
 202                                 abort = true;
 203                         }
 204                         else if (taxonomyFileName == "not open") { abort = true; }
 205
 206
 207                         namefile = validParameter.validFile(parameters, "name", false);
 208                         if (namefile == "not found") { namefile = "";  }
 209
 210                         else {
 211                                 m->splitAtDash(namefile, namefileNames);
 212
 213                                 //go through files and make sure they are good, if not, then disregard them
 214                                 for (int i = 0; i < namefileNames.size(); i++) {
 215                                         if (inputDir != "") {
 216                                                 string path = m->hasPath(namefileNames[i]);
 217                                                 //if the user has not given a path then, add inputdir. else leave path alone.
 218                                                 if (path == "") {       namefileNames[i] = inputDir + namefileNames[i];         }
 219                                         }
 220                                         int ableToOpen;
 221
 222                                         ifstream in;
 223                                         ableToOpen = m->openInputFile(namefileNames[i], in, "noerror");
 224
 225                                         //if you can't open it, try default location
 226                                         if (ableToOpen == 1) {
 227                                                 if (m->getDefaultPath() != "") { //default path is set
 228                                                         string tryPath = m->getDefaultPath() + m->getSimpleName(namefileNames[i]);
 229                                                         m->mothurOut("Unable to open " + namefileNames[i] + ". Trying default " + tryPath); m->mothurOutEndLine();
 230                                                         ifstream in2;
 231                                                         ableToOpen = m->openInputFile(tryPath, in2, "noerror");
 232                                                         in2.close();
 233                                                         namefileNames[i] = tryPath;
 234                                                 }
 235                                         }
 236
 237                                         if (ableToOpen == 1) {
 238                                                 if (m->getOutputDir() != "") { //default path is set
 239                                                         string tryPath = m->getOutputDir() + m->getSimpleName(namefileNames[i]);
 240                                                         m->mothurOut("Unable to open " + namefileNames[i] + ". Trying output directory " + tryPath); m->mothurOutEndLine();
 241                                                         ifstream in2;
 242                                                         ableToOpen = m->openInputFile(tryPath, in2, "noerror");
 243                                                         in2.close();
 244                                                         namefileNames[i] = tryPath;
 245                                                 }
 246                                         }
 247                                         in.close();
 248
 249                                         if (ableToOpen == 1) {
 250                                                 m->mothurOut("Unable to open " + namefileNames[i] + ". It will be disregarded."); m->mothurOutEndLine();  abort = true;
 251                                                 //erase from file list
 252                                                 namefileNames.erase(namefileNames.begin()+i);
 253                                                 i--;
 254                                         }
 255
 256                                 }
 257                         }
 258
 259                         if (namefile != "") {
 260                                 if (namefileNames.size() != fastaFileNames.size()) { abort = true; m->mothurOut("If you provide a name file, you must have one for each fasta file."); m->mothurOutEndLine(); }
 261                         }
 262
 263                         groupfile = validParameter.validFile(parameters, "group", false);
 264                         if (groupfile == "not found") { groupfile = "";  }
 265                         else {
 266                                 m->splitAtDash(groupfile, groupfileNames);
 267
 268                                 //go through files and make sure they are good, if not, then disregard them
 269                                 for (int i = 0; i < groupfileNames.size(); i++) {
 270                                         if (inputDir != "") {
 271                                                 string path = m->hasPath(groupfileNames[i]);
 272                                                 //if the user has not given a path then, add inputdir. else leave path alone.
 273                                                 if (path == "") {       groupfileNames[i] = inputDir + groupfileNames[i];               }
 274                                         }
 275                                         int ableToOpen;
 276
 277                                         ifstream in;
 278                                         ableToOpen = m->openInputFile(groupfileNames[i], in, "noerror");
 279
 280                                         //if you can't open it, try default location
 281                                         if (ableToOpen == 1) {
 282                                                 if (m->getDefaultPath() != "") { //default path is set
 283                                                         string tryPath = m->getDefaultPath() + m->getSimpleName(groupfileNames[i]);
 284                                                         m->mothurOut("Unable to open " + groupfileNames[i] + ". Trying default " + tryPath); m->mothurOutEndLine();
 285                                                         ifstream in2;
 286                                                         ableToOpen = m->openInputFile(tryPath, in2, "noerror");
 287                                                         in2.close();
 288                                                         groupfileNames[i] = tryPath;
 289                                                 }
 290                                         }
 291
 292                                         if (ableToOpen == 1) {
 293                                                 if (m->getOutputDir() != "") { //default path is set
 294                                                         string tryPath = m->getOutputDir() + m->getSimpleName(groupfileNames[i]);
 295                                                         m->mothurOut("Unable to open " + groupfileNames[i] + ". Trying output directory " + tryPath); m->mothurOutEndLine();
 296                                                         ifstream in2;
 297                                                         ableToOpen = m->openInputFile(tryPath, in2, "noerror");
 298                                                         in2.close();
 299                                                         groupfileNames[i] = tryPath;
 300                                                 }
 301                                         }
 302
 303                                         in.close();
 304
 305                                         if (ableToOpen == 1) {
 306                                                 m->mothurOut("Unable to open " + groupfileNames[i] + ". It will be disregarded."); m->mothurOutEndLine(); groupfileNames[i] = "";
 307                                                 //erase from file list
 308                                                 groupfileNames.erase(groupfileNames.begin()+i);
 309                                                 i--;
 310                                         }
 311                                 }
 312                         }
 313
 314                         if (groupfile != "") {
 315                                 if (groupfileNames.size() != fastaFileNames.size()) { abort = true; m->mothurOut("If you provide a group file, you must have one for each fasta file."); m->mothurOutEndLine(); }
 316                         }else {
 317                                 for (int i = 0; i < fastaFileNames.size(); i++) {  groupfileNames.push_back("");  }
 318                         }
 319
 320                         //check for optional parameter and set defaults
 321                         // ...at some point should added some additional type checking...
 322                         string temp;
 323                         temp = validParameter.validFile(parameters, "ksize", false);            if (temp == "not found"){       temp = "8";                             }
 324                         convert(temp, kmerSize);
 325
 326                         temp = validParameter.validFile(parameters, "processors", false);       if (temp == "not found"){       temp = "1";                             }
 327                         convert(temp, processors);
 328
 329                         search = validParameter.validFile(parameters, "search", false);         if (search == "not found"){     search = "kmer";                }
 330
 331                         method = validParameter.validFile(parameters, "method", false);         if (method == "not found"){     method = "bayesian";    }
 332
 333                         temp = validParameter.validFile(parameters, "match", false);            if (temp == "not found"){       temp = "1.0";                   }
 334                         convert(temp, match);
 335
 336                         temp = validParameter.validFile(parameters, "mismatch", false);         if (temp == "not found"){       temp = "-1.0";                  }
 337                         convert(temp, misMatch);
 338
 339                         temp = validParameter.validFile(parameters, "gapopen", false);          if (temp == "not found"){       temp = "-2.0";                  }
 340                         convert(temp, gapOpen);
 341
 342                         temp = validParameter.validFile(parameters, "gapextend", false);        if (temp == "not found"){       temp = "-1.0";                  }
 343                         convert(temp, gapExtend);
 344
 345                         temp = validParameter.validFile(parameters, "numwanted", false);        if (temp == "not found"){       temp = "10";                    }
 346                         convert(temp, numWanted);
 347
 348                         temp = validParameter.validFile(parameters, "cutoff", false);           if (temp == "not found"){       temp = "0";                             }
 349                         convert(temp, cutoff);
 350
 351                         temp = validParameter.validFile(parameters, "probs", false);            if (temp == "not found"){       temp = "true";                  }
 352                         probs = m->isTrue(temp);
 353
 354                         temp = validParameter.validFile(parameters, "iters", false);            if (temp == "not found") { temp = "100";                        }
 355                         convert(temp, iters);
 356
 357
 358
 359                         if ((method == "bayesian") && (search != "kmer"))  {
 360                                 m->mothurOut("The bayesian method requires the kmer search." + search + "will be disregarded." ); m->mothurOutEndLine();
 361                                 search = "kmer";
 362                         }
 363                 }
 364
 365         }
 366         catch(exception& e) {
 367                 m->errorOut(e, "ClassifySeqsCommand", "ClassifySeqsCommand");
 368                 exit(1);
 369         }
 370 }
 371
 372 //**********************************************************************************************************************
 373
 374 ClassifySeqsCommand::~ClassifySeqsCommand(){
 375
 376         if (abort == false) {
 377                 for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear();
 378         }
 379 }
 380
 381 //**********************************************************************************************************************
 382
 383 void ClassifySeqsCommand::help(){
 384         try {
 385                 m->mothurOut("The classify.seqs command reads a fasta file containing sequences and creates a .taxonomy file and a .tax.summary file.\n");
 386                 m->mothurOut("The classify.seqs command parameters are template, fasta, name, search, ksize, method, taxonomy, processors, match, mismatch, gapopen, gapextend, numwanted and probs.\n");
 387                 m->mothurOut("The template, fasta and taxonomy parameters are required. You may enter multiple fasta files by separating their names with dashes. ie. fasta=abrecovery.fasta-amzon.fasta \n");
 388                 m->mothurOut("The search parameter allows you to specify the method to find most similar template.  Your options are: suffix, kmer, blast and distance. The default is kmer.\n");
 389                 m->mothurOut("The name parameter allows you add a names file with your fasta file, if you enter multiple fasta files, you must enter matching names files for them.\n");
 390                 m->mothurOut("The group parameter allows you add a group file so you can have the summary totals broken up by group.\n");
 391                 m->mothurOut("The method parameter allows you to specify classification method to use.  Your options are: bayesian and knn. The default is bayesian.\n");
 392                 m->mothurOut("The ksize parameter allows you to specify the kmer size for finding most similar template to candidate.  The default is 8.\n");
 393                 m->mothurOut("The processors parameter allows you to specify the number of processors to use. The default is 1.\n");
 394                 #ifdef USE_MPI
 395                 m->mothurOut("When using MPI, the processors parameter is set to the number of MPI processes running. \n");
 396                 #endif
 397                 m->mothurOut("The match parameter allows you to specify the bonus for having the same base. The default is 1.0.\n");
 398                 m->mothurOut("The mistmatch parameter allows you to specify the penalty for having different bases.  The default is -1.0.\n");
 399                 m->mothurOut("The gapopen parameter allows you to specify the penalty for opening a gap in an alignment. The default is -2.0.\n");
 400                 m->mothurOut("The gapextend parameter allows you to specify the penalty for extending a gap in an alignment.  The default is -1.0.\n");
 401                 m->mothurOut("The numwanted parameter allows you to specify the number of sequence matches you want with the knn method.  The default is 10.\n");
 402                 m->mothurOut("The cutoff parameter allows you to specify a bootstrap confidence threshold for your taxonomy.  The default is 0.\n");
 403                 m->mothurOut("The probs parameter shuts off the bootstrapping results for the bayesian method. The default is true, meaning you want the bootstrapping to be shown.\n");
 404                 m->mothurOut("The iters parameter allows you to specify how many iterations to do when calculating the bootstrap confidence score for your taxonomy with the bayesian method.  The default is 100.\n");
 405                 m->mothurOut("The classify.seqs command should be in the following format: \n");
 406                 m->mothurOut("classify.seqs(template=yourTemplateFile, fasta=yourFastaFile, method=yourClassificationMethod, search=yourSearchmethod, ksize=yourKmerSize, taxonomy=yourTaxonomyFile, processors=yourProcessors) \n");
 407                 m->mothurOut("Example classify.seqs(fasta=amazon.fasta, template=core.filtered, method=knn, search=gotoh, ksize=8, processors=2)\n");
 408                 m->mothurOut("The .taxonomy file consists of 2 columns: 1 = your sequence name, 2 = the taxonomy for your sequence. \n");
 409                 m->mothurOut("The .tax.summary is a summary of the different taxonomies represented in your fasta file. \n");
 410                 m->mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFastaFile).\n\n");
 411         }
 412         catch(exception& e) {
 413                 m->errorOut(e, "ClassifySeqsCommand", "help");
 414                 exit(1);
 415         }
 416 }
 417
 418
 419 //**********************************************************************************************************************
 420
 421 int ClassifySeqsCommand::execute(){
 422         try {
 423                 if (abort == true) {    return 0;       }
 424
 425                 if(method == "bayesian"){       classify = new Bayesian(taxonomyFileName, templateFileName, search, kmerSize, cutoff, iters);           }
 426                 else if(method == "knn"){       classify = new Knn(taxonomyFileName, templateFileName, search, kmerSize, gapOpen, gapExtend, match, misMatch, numWanted);                               }
 427                 else {
 428                         m->mothurOut(search + " is not a valid method option. I will run the command using bayesian.");
 429                         m->mothurOutEndLine();
 430                         classify = new Bayesian(taxonomyFileName, templateFileName, search, kmerSize, cutoff, iters);
 431                 }
 432
 433                 if (m->control_pressed) { delete classify; return 0; }
 434
 435
 436                 for (int s = 0; s < fastaFileNames.size(); s++) {
 437
 438                         m->mothurOut("Classifying sequences from " + fastaFileNames[s] + " ..." ); m->mothurOutEndLine();
 439
 440                         string RippedTaxName = m->getRootName(m->getSimpleName(taxonomyFileName));
 441                         RippedTaxName = m->getExtension(RippedTaxName.substr(0, RippedTaxName.length()-1));
 442                         if (RippedTaxName[0] == '.') { RippedTaxName = RippedTaxName.substr(1, RippedTaxName.length()); }
 443                         RippedTaxName +=  ".";
 444
 445                         if (outputDir == "") { outputDir += m->hasPath(fastaFileNames[s]); }
 446                         string newTaxonomyFile = outputDir + m->getRootName(m->getSimpleName(fastaFileNames[s])) + RippedTaxName + "taxonomy";
 447                         string tempTaxonomyFile = outputDir + m->getRootName(m->getSimpleName(fastaFileNames[s])) + "taxonomy.temp";
 448                         string taxSummary = outputDir + m->getRootName(m->getSimpleName(fastaFileNames[s])) + RippedTaxName + "tax.summary";
 449
 450                         if ((method == "knn") && (search == "distance")) {
 451                                 string DistName = outputDir + m->getRootName(m->getSimpleName(fastaFileNames[s])) + "match.dist";
 452                                 classify->setDistName(DistName);  outputNames.push_back(DistName); outputTypes["matchdist"].push_back(DistName);
 453                         }
 454
 455                         outputNames.push_back(newTaxonomyFile); outputTypes["taxonomy"].push_back(newTaxonomyFile);
 456                         outputNames.push_back(taxSummary);      outputTypes["taxsummary"].push_back(taxSummary);
 457
 458                         int start = time(NULL);
 459                         int numFastaSeqs = 0;
 460                         for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear();
 461
 462 #ifdef USE_MPI
 463                                 int pid, end, numSeqsPerProcessor;
 464                                 int tag = 2001;
 465                                 vector<unsigned long int> MPIPos;
 466
 467                                 MPI_Status status;
 468                                 MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
 469                                 MPI_Comm_size(MPI_COMM_WORLD, &processors);
 470
 471                                 MPI_File inMPI;
 472                                 MPI_File outMPINewTax;
 473                                 MPI_File outMPITempTax;
 474
 475                                 int outMode=MPI_MODE_CREATE|MPI_MODE_WRONLY;
 476                                 int inMode=MPI_MODE_RDONLY;
 477
 478                                 char outNewTax[1024];
 479                                 strcpy(outNewTax, newTaxonomyFile.c_str());
 480
 481                                 char outTempTax[1024];
 482                                 strcpy(outTempTax, tempTaxonomyFile.c_str());
 483
 484                                 char inFileName[1024];
 485                                 strcpy(inFileName, fastaFileNames[s].c_str());
 486
 487                                 MPI_File_open(MPI_COMM_WORLD, inFileName, inMode, MPI_INFO_NULL, &inMPI);  //comm, filename, mode, info, filepointer
 488                                 MPI_File_open(MPI_COMM_WORLD, outNewTax, outMode, MPI_INFO_NULL, &outMPINewTax);
 489                                 MPI_File_open(MPI_COMM_WORLD, outTempTax, outMode, MPI_INFO_NULL, &outMPITempTax);
 490
 491                                 if (m->control_pressed) { outputTypes.clear(); MPI_File_close(&inMPI);  MPI_File_close(&outMPINewTax);   MPI_File_close(&outMPITempTax);  delete classify; return 0;  }
 492
 493                                 if (pid == 0) { //you are the root process
 494
 495                                         MPIPos = m->setFilePosFasta(fastaFileNames[s], numFastaSeqs); //fills MPIPos, returns numSeqs
 496
 497                                         //send file positions to all processes
 498                                         for(int i = 1; i < processors; i++) {
 499                                                 MPI_Send(&numFastaSeqs, 1, MPI_INT, i, tag, MPI_COMM_WORLD);
 500                                                 MPI_Send(&MPIPos[0], (numFastaSeqs+1), MPI_LONG, i, tag, MPI_COMM_WORLD);
 501                                         }
 502
 503                                         //figure out how many sequences you have to align
 504                                         numSeqsPerProcessor = numFastaSeqs / processors;
 505                                         int startIndex =  pid * numSeqsPerProcessor;
 506                                         if(pid == (processors - 1)){    numSeqsPerProcessor = numFastaSeqs - pid * numSeqsPerProcessor;         }
 507
 508
 509                                         //align your part
 510                                         driverMPI(startIndex, numSeqsPerProcessor, inMPI, outMPINewTax, outMPITempTax, MPIPos);
 511
 512                                         if (m->control_pressed) {  outputTypes.clear(); MPI_File_close(&inMPI);  MPI_File_close(&outMPINewTax);   MPI_File_close(&outMPITempTax);  for (int i = 0; i < outputNames.size(); i++) {       remove(outputNames[i].c_str()); } delete classify; return 0;  }
 513
 514                                         for (int i = 1; i < processors; i++) {
 515                                                 int done;
 516                                                 MPI_Recv(&done, 1, MPI_INT, i, tag, MPI_COMM_WORLD, &status);
 517                                         }
 518                                 }else{ //you are a child process
 519                                         MPI_Recv(&numFastaSeqs, 1, MPI_INT, 0, tag, MPI_COMM_WORLD, &status);
 520                                         MPIPos.resize(numFastaSeqs+1);
 521                                         MPI_Recv(&MPIPos[0], (numFastaSeqs+1), MPI_LONG, 0, tag, MPI_COMM_WORLD, &status);
 522
 523                                         //figure out how many sequences you have to align
 524                                         numSeqsPerProcessor = numFastaSeqs / processors;
 525                                         int startIndex =  pid * numSeqsPerProcessor;
 526                                         if(pid == (processors - 1)){    numSeqsPerProcessor = numFastaSeqs - pid * numSeqsPerProcessor;         }
 527
 528
 529                                         //align your part
 530                                         driverMPI(startIndex, numSeqsPerProcessor, inMPI, outMPINewTax, outMPITempTax, MPIPos);
 531
 532                                         if (m->control_pressed) {  outputTypes.clear(); MPI_File_close(&inMPI);  MPI_File_close(&outMPINewTax);   MPI_File_close(&outMPITempTax);  delete classify; return 0;  }
 533
 534                                         int done = 0;
 535                                         MPI_Send(&done, 1, MPI_INT, 0, tag, MPI_COMM_WORLD);
 536                                 }
 537
 538                                 //close files
 539                                 MPI_File_close(&inMPI);
 540                                 MPI_File_close(&outMPINewTax);
 541                                 MPI_File_close(&outMPITempTax);
 542                                 MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case
 543
 544 #else
 545
 546                         vector<unsigned long int> positions = m->divideFile(fastaFileNames[s], processors);
 547
 548                         for (int i = 0; i < (positions.size()-1); i++) {
 549                                 lines.push_back(new linePair(positions[i], positions[(i+1)]));
 550                         }
 551
 552                 #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
 553                         if(processors == 1){
 554                                 numFastaSeqs = driver(lines[0], newTaxonomyFile, tempTaxonomyFile, fastaFileNames[s]);
 555                         }
 556                         else{
 557                                 processIDS.resize(0);
 558
 559                                 numFastaSeqs = createProcesses(newTaxonomyFile, tempTaxonomyFile, fastaFileNames[s]);
 560
 561                                 rename((newTaxonomyFile + toString(processIDS[0]) + ".temp").c_str(), newTaxonomyFile.c_str());
 562                                 rename((tempTaxonomyFile + toString(processIDS[0]) + ".temp").c_str(), tempTaxonomyFile.c_str());
 563
 564                                 for(int i=1;i<processors;i++){
 565                                         appendTaxFiles((newTaxonomyFile + toString(processIDS[i]) + ".temp"), newTaxonomyFile);
 566                                         appendTaxFiles((tempTaxonomyFile + toString(processIDS[i]) + ".temp"), tempTaxonomyFile);
 567                                         remove((newTaxonomyFile + toString(processIDS[i]) + ".temp").c_str());
 568                                         remove((tempTaxonomyFile + toString(processIDS[i]) + ".temp").c_str());
 569                                 }
 570
 571                         }
 572         #else
 573                         numFastaSeqs = driver(lines[0], newTaxonomyFile, tempTaxonomyFile, fastaFileNames[s]);
 574         #endif
 575 #endif
 576
 577                 m->mothurOutEndLine();
 578                 m->mothurOut("It took " + toString(time(NULL) - start) + " secs to classify " + toString(numFastaSeqs) + " sequences."); m->mothurOutEndLine(); m->mothurOutEndLine();
 579                 start = time(NULL);
 580
 581
 582                 #ifdef USE_MPI
 583                         if (pid == 0) {  //this part does not need to be paralellized
 584
 585                                 if(namefile != "") { m->mothurOut("Reading " + namefileNames[s] + "..."); cout.flush();  MPIReadNamesFile(namefileNames[s]);  m->mothurOut("  Done."); m->mothurOutEndLine(); }
 586                 #else
 587                         //read namefile
 588                         if(namefile != "") {
 589
 590                             m->mothurOut("Reading " + namefileNames[s] + "..."); cout.flush();
 591
 592                                 nameMap.clear(); //remove old names
 593
 594                                 ifstream inNames;
 595                                 m->openInputFile(namefileNames[s], inNames);
 596
 597                                 string firstCol, secondCol;
 598                                 while(!inNames.eof()) {
 599                                         inNames >> firstCol >> secondCol; m->gobble(inNames);
 600
 601                                         vector<string> temp;
 602                                         m->splitAtComma(secondCol, temp);
 603
 604                                         nameMap[firstCol] = temp;
 605                                 }
 606                                 inNames.close();
 607
 608                                 m->mothurOut("  Done."); m->mothurOutEndLine();
 609                         }
 610                 #endif
 611
 612                         string group = "";
 613                         if (groupfile != "") {  group = groupfileNames[s]; }
 614
 615                         PhyloSummary taxaSum(taxonomyFileName, group);
 616
 617                         if (m->control_pressed) { outputTypes.clear();  for (int i = 0; i < outputNames.size(); i++) {  remove(outputNames[i].c_str()); } delete classify; return 0; }
 618
 619                         if (namefile == "") {  taxaSum.summarize(tempTaxonomyFile);  }
 620                         else {
 621                                 ifstream in;
 622                                 m->openInputFile(tempTaxonomyFile, in);
 623
 624                                 //read in users taxonomy file and add sequences to tree
 625                                 string name, taxon;
 626
 627                                 while(!in.eof()){
 628                                         in >> name >> taxon; m->gobble(in);
 629
 630                                         itNames = nameMap.find(name);
 631
 632                                         if (itNames == nameMap.end()) {
 633                                                 m->mothurOut(name + " is not in your name file please correct."); m->mothurOutEndLine(); exit(1);
 634                                         }else{
 635                                                 for (int i = 0; i < itNames->second.size(); i++) {
 636                                                         taxaSum.addSeqToTree(itNames->second[i], taxon);  //add it as many times as there are identical seqs
 637                                                 }
 638                                                 itNames->second.clear();
 639                                                 nameMap.erase(itNames->first);
 640                                         }
 641                                 }
 642                                 in.close();
 643                         }
 644                         remove(tempTaxonomyFile.c_str());
 645
 646                         if (m->control_pressed) {  outputTypes.clear(); for (int i = 0; i < outputNames.size(); i++) {  remove(outputNames[i].c_str()); } delete classify; return 0; }
 647
 648                         //print summary file
 649                         ofstream outTaxTree;
 650                         m->openOutputFile(taxSummary, outTaxTree);
 651                         taxaSum.print(outTaxTree);
 652                         outTaxTree.close();
 653
 654                         //output taxonomy with the unclassified bins added
 655                         ifstream inTax;
 656                         m->openInputFile(newTaxonomyFile, inTax);
 657
 658                         ofstream outTax;
 659                         string unclass = newTaxonomyFile + ".unclass.temp";
 660                         m->openOutputFile(unclass, outTax);
 661
 662                         //get maxLevel from phylotree so you know how many 'unclassified's to add
 663                         int maxLevel = taxaSum.getMaxLevel();
 664
 665                         //read taxfile - this reading and rewriting is done to preserve the confidence scores.
 666                         string name, taxon;
 667                         while (!inTax.eof()) {
 668                                 if (m->control_pressed) { outputTypes.clear();  for (int i = 0; i < outputNames.size(); i++) {  remove(outputNames[i].c_str()); } remove(unclass.c_str()); delete classify; return 0; }
 669
 670                                 inTax >> name >> taxon; m->gobble(inTax);
 671
 672                                 string newTax = addUnclassifieds(taxon, maxLevel);
 673
 674                                 outTax << name << '\t' << newTax << endl;
 675                         }
 676                         inTax.close();
 677                         outTax.close();
 678
 679                         remove(newTaxonomyFile.c_str());
 680                         rename(unclass.c_str(), newTaxonomyFile.c_str());
 681
 682                         m->mothurOutEndLine();
 683                         m->mothurOut("It took " + toString(time(NULL) - start) + " secs to create the summary file for " + toString(numFastaSeqs) + " sequences."); m->mothurOutEndLine(); m->mothurOutEndLine();
 684
 685                         #ifdef USE_MPI
 686                                 }
 687                         #endif
 688
 689                         m->mothurOutEndLine();
 690                         m->mothurOut("Output File Names: "); m->mothurOutEndLine();
 691                         for (int i = 0; i < outputNames.size(); i++) {  m->mothurOut(outputNames[i]); m->mothurOutEndLine();    }
 692                         m->mothurOutEndLine();
 693                 }
 694
 695                 delete classify;
 696                 return 0;
 697         }
 698         catch(exception& e) {
 699                 m->errorOut(e, "ClassifySeqsCommand", "execute");
 700                 exit(1);
 701         }
 702 }
 703
 704 /**************************************************************************************************/
 705 string ClassifySeqsCommand::addUnclassifieds(string tax, int maxlevel) {
 706         try{
 707                 string newTax, taxon;
 708                 int level = 0;
 709
 710                 //keep what you have counting the levels
 711                 while (tax.find_first_of(';') != -1) {
 712                         //get taxon
 713                         taxon = tax.substr(0,tax.find_first_of(';'))+';';
 714                         tax = tax.substr(tax.find_first_of(';')+1, tax.length());
 715                         newTax += taxon;
 716                         level++;
 717                 }
 718
 719                 //add "unclassified" until you reach maxLevel
 720                 while (level < maxlevel) {
 721                         newTax += "unclassified;";
 722                         level++;
 723                 }
 724
 725                 return newTax;
 726         }
 727         catch(exception& e) {
 728                 m->errorOut(e, "ClassifySeqsCommand", "addUnclassifieds");
 729                 exit(1);
 730         }
 731 }
 732
 733 /**************************************************************************************************/
 734
 735 int ClassifySeqsCommand::createProcesses(string taxFileName, string tempTaxFile, string filename) {
 736         try {
 737 #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
 738                 int process = 0;
 739                 int num = 0;
 740
 741                 //loop through and create all the processes you want
 742                 while (process != processors) {
 743                         int pid = fork();
 744
 745                         if (pid > 0) {
 746                                 processIDS.push_back(pid);  //create map from line number to pid so you can append files in correct order later
 747                                 process++;
 748                         }else if (pid == 0){
 749                                 num = driver(lines[process], taxFileName + toString(getpid()) + ".temp", tempTaxFile + toString(getpid()) + ".temp", filename);
 750
 751                                 //pass numSeqs to parent
 752                                 ofstream out;
 753                                 string tempFile = filename + toString(getpid()) + ".num.temp";
 754                                 m->openOutputFile(tempFile, out);
 755                                 out << num << endl;
 756                                 out.close();
 757
 758                                 exit(0);
 759                         }else { m->mothurOut("unable to spawn the necessary processes."); m->mothurOutEndLine(); exit(0); }
 760                 }
 761
 762                 //force parent to wait until all the processes are done
 763                 for (int i=0;i<processors;i++) {
 764                         int temp = processIDS[i];
 765                         wait(&temp);
 766                 }
 767
 768                 for (int i = 0; i < processIDS.size(); i++) {
 769                         ifstream in;
 770                         string tempFile =  filename + toString(processIDS[i]) + ".num.temp";
 771                         m->openInputFile(tempFile, in);
 772                         if (!in.eof()) { int tempNum = 0; in >> tempNum; num += tempNum; }
 773                         in.close(); remove(tempFile.c_str());
 774                 }
 775
 776                 return num;
 777 #endif
 778         }
 779         catch(exception& e) {
 780                 m->errorOut(e, "ClassifySeqsCommand", "createProcesses");
 781                 exit(1);
 782         }
 783 }
 784 /**************************************************************************************************/
 785
 786 void ClassifySeqsCommand::appendTaxFiles(string temp, string filename) {
 787         try{
 788
 789                 ofstream output;
 790                 ifstream input;
 791                 m->openOutputFileAppend(filename, output);
 792                 m->openInputFile(temp, input);
 793
 794                 while(char c = input.get()){
 795                         if(input.eof())         {       break;                  }
 796                         else                            {       output << c;    }
 797                 }
 798
 799                 input.close();
 800                 output.close();
 801         }
 802         catch(exception& e) {
 803                 m->errorOut(e, "ClassifySeqsCommand", "appendTaxFiles");
 804                 exit(1);
 805         }
 806 }
 807
 808 //**********************************************************************************************************************
 809
 810 int ClassifySeqsCommand::driver(linePair* filePos, string taxFName, string tempTFName, string filename){
 811         try {
 812                 ofstream outTax;
 813                 m->openOutputFile(taxFName, outTax);
 814
 815                 ofstream outTaxSimple;
 816                 m->openOutputFile(tempTFName, outTaxSimple);
 817
 818                 ifstream inFASTA;
 819                 m->openInputFile(filename, inFASTA);
 820
 821                 string taxonomy;
 822
 823                 inFASTA.seekg(filePos->start);
 824
 825                 bool done = false;
 826                 int count = 0;
 827
 828                 while (!done) {
 829                         if (m->control_pressed) { return 0; }
 830
 831                         Sequence* candidateSeq = new Sequence(inFASTA); m->gobble(inFASTA);
 832
 833                         if (candidateSeq->getName() != "") {
 834                                 taxonomy = classify->getTaxonomy(candidateSeq);
 835
 836                                 if (m->control_pressed) { delete candidateSeq; return 0; }
 837
 838                                 if (taxonomy != "bad seq") {
 839                                         //output confidence scores or not
 840                                         if (probs) {
 841                                                 outTax << candidateSeq->getName() << '\t' << taxonomy << endl;
 842                                         }else{
 843                                                 outTax << candidateSeq->getName() << '\t' << classify->getSimpleTax() << endl;
 844                                         }
 845
 846                                         outTaxSimple << candidateSeq->getName() << '\t' << classify->getSimpleTax() << endl;
 847                                 }
 848                                 count++;
 849                         }
 850                         delete candidateSeq;
 851
 852                         #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
 853                                 unsigned long int pos = inFASTA.tellg();
 854                                 if ((pos == -1) || (pos >= filePos->end)) { break; }
 855                         #else
 856                                 if (inFASTA.eof()) { break; }
 857                         #endif
 858
 859                         //report progress
 860                         if((count) % 100 == 0){ m->mothurOut("Processing sequence: " + toString(count)); m->mothurOutEndLine();         }
 861                 }
 862                 //report progress
 863                 if((count) % 100 != 0){ m->mothurOut("Processing sequence: " + toString(count)); m->mothurOutEndLine();         }
 864
 865                 inFASTA.close();
 866                 outTax.close();
 867                 outTaxSimple.close();
 868
 869                 return count;
 870         }
 871         catch(exception& e) {
 872                 m->errorOut(e, "ClassifySeqsCommand", "driver");
 873                 exit(1);
 874         }
 875 }
 876 //**********************************************************************************************************************
 877 #ifdef USE_MPI
 878 int ClassifySeqsCommand::driverMPI(int start, int num, MPI_File& inMPI, MPI_File& newFile, MPI_File& tempFile, vector<unsigned long int>& MPIPos){
 879         try {
 880                 MPI_Status statusNew;
 881                 MPI_Status statusTemp;
 882                 MPI_Status status;
 883
 884                 int pid;
 885                 MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
 886
 887                 string taxonomy;
 888                 string outputString;
 889
 890                 for(int i=0;i<num;i++){
 891
 892                         if (m->control_pressed) { return 0; }
 893
 894                         //read next sequence
 895                         int length = MPIPos[start+i+1] - MPIPos[start+i];
 896                         char* buf4 = new char[length];
 897                         MPI_File_read_at(inMPI, MPIPos[start+i], buf4, length, MPI_CHAR, &status);
 898
 899                         string tempBuf = buf4;
 900                         if (tempBuf.length() > length) { tempBuf = tempBuf.substr(0, length);  }
 901                         istringstream iss (tempBuf,istringstream::in);
 902                         delete buf4;
 903
 904                         Sequence* candidateSeq = new Sequence(iss);
 905
 906                         if (candidateSeq->getName() != "") {
 907                                 taxonomy = classify->getTaxonomy(candidateSeq);
 908
 909                                 if (taxonomy != "bad seq") {
 910                                         //output confidence scores or not
 911                                         if (probs) {
 912                                                 outputString =  candidateSeq->getName() + "\t" + taxonomy + "\n";
 913                                         }else{
 914                                                 outputString =  candidateSeq->getName() + "\t" + classify->getSimpleTax() + "\n";
 915                                         }
 916
 917                                         int length = outputString.length();
 918                                         char* buf2 = new char[length];
 919                                         memcpy(buf2, outputString.c_str(), length);
 920
 921                                         MPI_File_write_shared(newFile, buf2, length, MPI_CHAR, &statusNew);
 922                                         delete buf2;
 923
 924                                         outputString =  candidateSeq->getName() + "\t" + classify->getSimpleTax() + "\n";
 925                                         length = outputString.length();
 926                                         char* buf = new char[length];
 927                                         memcpy(buf, outputString.c_str(), length);
 928
 929                                         MPI_File_write_shared(tempFile, buf, length, MPI_CHAR, &statusTemp);
 930                                         delete buf;
 931                                 }
 932                         }
 933                         delete candidateSeq;
 934
 935                         if((i+1) % 100 == 0){   cout << "Classifying sequence " << (i+1) << endl;       }
 936                 }
 937
 938                 if(num % 100 != 0){     cout << "Classifying sequence " << (num) << endl;       }
 939
 940
 941                 return 1;
 942         }
 943         catch(exception& e) {
 944                 m->errorOut(e, "ClassifySeqsCommand", "driverMPI");
 945                 exit(1);
 946         }
 947 }
 948
 949 //**********************************************************************************************************************
 950 int ClassifySeqsCommand::MPIReadNamesFile(string nameFilename){
 951         try {
 952
 953                 nameMap.clear(); //remove old names
 954
 955                 MPI_File inMPI;
 956                 MPI_Offset size;
 957                 MPI_Status status;
 958
 959                 //char* inFileName = new char[nameFilename.length()];
 960                 //memcpy(inFileName, nameFilename.c_str(), nameFilename.length());
 961
 962                 char inFileName[1024];
 963                 strcpy(inFileName, nameFilename.c_str());
 964
 965                 MPI_File_open(MPI_COMM_WORLD, inFileName, MPI_MODE_RDONLY, MPI_INFO_NULL, &inMPI);
 966                 MPI_File_get_size(inMPI, &size);
 967                 //delete inFileName;
 968
 969                 char* buffer = new char[size];
 970                 MPI_File_read(inMPI, buffer, size, MPI_CHAR, &status);
 971
 972                 string tempBuf = buffer;
 973                 if (tempBuf.length() > size) { tempBuf = tempBuf.substr(0, size);  }
 974                 istringstream iss (tempBuf,istringstream::in);
 975                 delete buffer;
 976
 977                 string firstCol, secondCol;
 978                 while(!iss.eof()) {
 979                         iss >> firstCol >> secondCol; m->gobble(iss);
 980
 981                         vector<string> temp;
 982                         m->splitAtComma(secondCol, temp);
 983
 984                         nameMap[firstCol] = temp;
 985                 }
 986
 987                 MPI_File_close(&inMPI);
 988
 989                 return 1;
 990         }
 991         catch(exception& e) {
 992                 m->errorOut(e, "ClassifySeqsCommand", "MPIReadNamesFile");
 993                 exit(1);
 994         }
 995 }
 996 #endif
 997 /**************************************************************************************************/