chimeraslayercommand.cpp

   1 /*
   2  *  chimeraslayercommand.cpp
   3  *  Mothur
   4  *
   5  *  Created by westcott on 3/31/10.
   6  *  Copyright 2010 Schloss Lab. All rights reserved.
   7  *
   8  */
   9
  10 #include "chimeraslayercommand.h"
  11 #include "chimeraslayer.h"
  12
  13
  14 //***************************************************************************************************************
  15
  16 ChimeraSlayerCommand::ChimeraSlayerCommand(string option)  {
  17         try {
  18                 abort = false;
  19
  20                 //allow user to run help
  21                 if(option == "help") { help(); abort = true; }
  22
  23                 else {
  24                         //valid paramters for this command
  25                         string Array[] =  {"fasta", "processors", "window", "template","numwanted", "ksize", "match","mismatch",
  26                         "divergence", "minsim","mincov","minbs", "minsnp","parents", "iters","outputdir","inputdir", "search","realign" };
  27                         vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
  28
  29                         OptionParser parser(option);
  30                         map<string,string> parameters = parser.getParameters();
  31
  32                         ValidParameters validParameter("chimera.slayer");
  33                         map<string,string>::iterator it;
  34
  35                         //check to make sure all parameters are valid for command
  36                         for (it = parameters.begin(); it != parameters.end(); it++) {
  37                                 if (validParameter.isValidParameter(it->first, myArray, it->second) != true) {  abort = true;  }
  38                         }
  39
  40                         //if the user changes the input directory command factory will send this info to us in the output parameter
  41                         string inputDir = validParameter.validFile(parameters, "inputdir", false);
  42                         if (inputDir == "not found"){   inputDir = "";          }
  43                         else {
  44                                 string path;
  45                                 it = parameters.find("template");
  46                                 //user has given a template file
  47                                 if(it != parameters.end()){
  48                                         path = m->hasPath(it->second);
  49                                         //if the user has not given a path then, add inputdir. else leave path alone.
  50                                         if (path == "") {       parameters["template"] = inputDir + it->second;         }
  51                                 }
  52                         }
  53
  54
  55                         //check for required parameters
  56                         fastafile = validParameter.validFile(parameters, "fasta", false);
  57                         if (fastafile == "not found") { fastafile = ""; m->mothurOut("fasta is a required parameter for the chimera.slayer command."); m->mothurOutEndLine(); abort = true;  }
  58                         else {
  59                                 m->splitAtDash(fastafile, fastaFileNames);
  60
  61                                 //go through files and make sure they are good, if not, then disregard them
  62                                 for (int i = 0; i < fastaFileNames.size(); i++) {
  63                                         if (inputDir != "") {
  64                                                 string path = m->hasPath(fastaFileNames[i]);
  65                                                 //if the user has not given a path then, add inputdir. else leave path alone.
  66                                                 if (path == "") {       fastaFileNames[i] = inputDir + fastaFileNames[i];               }
  67                                         }
  68
  69                                         int ableToOpen;
  70                                         ifstream in;
  71
  72                                         ableToOpen = m->openInputFile(fastaFileNames[i], in, "noerror");
  73
  74                                         //if you can't open it, try default location
  75                                         if (ableToOpen == 1) {
  76                                                 if (m->getDefaultPath() != "") { //default path is set
  77                                                         string tryPath = m->getDefaultPath() + m->getSimpleName(fastaFileNames[i]);
  78                                                         m->mothurOut("Unable to open " + fastaFileNames[i] + ". Trying default " + tryPath); m->mothurOutEndLine();
  79                                                         ableToOpen = m->openInputFile(tryPath, in, "noerror");
  80                                                         fastaFileNames[i] = tryPath;
  81                                                 }
  82                                         }
  83                                         in.close();
  84
  85                                         if (ableToOpen == 1) {
  86                                                 m->mothurOut("Unable to open " + fastaFileNames[i] + ". It will be disregarded."); m->mothurOutEndLine();
  87                                                 //erase from file list
  88                                                 fastaFileNames.erase(fastaFileNames.begin()+i);
  89                                                 i--;
  90                                         }
  91                                 }
  92
  93                                 //make sure there is at least one valid file left
  94                                 if (fastaFileNames.size() == 0) { m->mothurOut("no valid files."); m->mothurOutEndLine(); abort = true; }
  95                         }
  96
  97                         //if the user changes the output directory command factory will send this info to us in the output parameter
  98                         outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){
  99                                 outputDir = "";
 100                                 outputDir += m->hasPath(fastafile); //if user entered a file with a path then preserve it
 101                         }
 102
 103                         templatefile = validParameter.validFile(parameters, "template", true);
 104                         if (templatefile == "not open") { abort = true; }
 105                         else if (templatefile == "not found") { templatefile = "";  m->mothurOut("template is a required parameter for the chimera.slayer command."); m->mothurOutEndLine(); abort = true;  }
 106
 107                         string temp = validParameter.validFile(parameters, "processors", false);                if (temp == "not found") { temp = "1"; }
 108                         convert(temp, processors);
 109
 110                         temp = validParameter.validFile(parameters, "ksize", false);                    if (temp == "not found") { temp = "7"; }
 111                         convert(temp, ksize);
 112
 113                         temp = validParameter.validFile(parameters, "window", false);                   if (temp == "not found") { temp = "50"; }
 114                         convert(temp, window);
 115
 116                         temp = validParameter.validFile(parameters, "match", false);                    if (temp == "not found") { temp = "5"; }
 117                         convert(temp, match);
 118
 119                         temp = validParameter.validFile(parameters, "mismatch", false);                 if (temp == "not found") { temp = "-4"; }
 120                         convert(temp, mismatch);
 121
 122                         temp = validParameter.validFile(parameters, "divergence", false);               if (temp == "not found") { temp = "1.007"; }
 123                         convert(temp, divR);
 124
 125                         temp = validParameter.validFile(parameters, "minsim", false);                   if (temp == "not found") { temp = "90"; }
 126                         convert(temp, minSimilarity);
 127
 128                         temp = validParameter.validFile(parameters, "mincov", false);                   if (temp == "not found") { temp = "70"; }
 129                         convert(temp, minCoverage);
 130
 131                         temp = validParameter.validFile(parameters, "minbs", false);                    if (temp == "not found") { temp = "90"; }
 132                         convert(temp, minBS);
 133
 134                         temp = validParameter.validFile(parameters, "minsnp", false);                   if (temp == "not found") { temp = "100"; }
 135                         convert(temp, minSNP);
 136
 137                         temp = validParameter.validFile(parameters, "parents", false);                  if (temp == "not found") { temp = "3"; }
 138                         convert(temp, parents);
 139
 140                         temp = validParameter.validFile(parameters, "realign", false);                  if (temp == "not found") { temp = "f"; }
 141                         realign = m->isTrue(temp);
 142
 143                         search = validParameter.validFile(parameters, "search", false);                 if (search == "not found") { search = "distance"; }
 144
 145                         temp = validParameter.validFile(parameters, "iters", false);                    if (temp == "not found") { temp = "100"; }
 146                         convert(temp, iters);
 147
 148                         temp = validParameter.validFile(parameters, "increment", false);                if (temp == "not found") { temp = "5"; }
 149                         convert(temp, increment);
 150
 151                         temp = validParameter.validFile(parameters, "numwanted", false);                if (temp == "not found") { temp = "15"; }
 152                         convert(temp, numwanted);
 153
 154                         if ((search != "distance") && (search != "blast") && (search != "kmer")) { m->mothurOut(search + " is not a valid search."); m->mothurOutEndLine(); abort = true;  }
 155                 }
 156         }
 157         catch(exception& e) {
 158                 m->errorOut(e, "ChimeraSlayerCommand", "ChimeraSlayerCommand");
 159                 exit(1);
 160         }
 161 }
 162 //**********************************************************************************************************************
 163
 164 void ChimeraSlayerCommand::help(){
 165         try {
 166
 167                 m->mothurOut("The chimera.slayer command reads a fastafile and templatefile and outputs potentially chimeric sequences.\n");
 168                 m->mothurOut("This command was modeled after the chimeraSlayer written by the Broad Institute.\n");
 169                 m->mothurOut("The chimera.slayer command parameters are fasta, template, processors, ksize, window, match, mismatch, divergence. minsim, mincov, minbs, minsnp, parents, search, iters, increment and numwanted.\n"); //realign,
 170                 m->mothurOut("The fasta parameter allows you to enter the fasta file containing your potentially chimeric sequences, and is required. \n");
 171                 m->mothurOut("You may enter multiple fasta files by separating their names with dashes. ie. fasta=abrecovery.fasta-amazon.fasta \n");
 172                 m->mothurOut("The template parameter allows you to enter a template file containing known non-chimeric sequences, and is required. \n");
 173                 m->mothurOut("The processors parameter allows you to specify how many processors you would like to use.  The default is 1. \n");
 174                 #ifdef USE_MPI
 175                 m->mothurOut("When using MPI, the processors parameter is set to the number of MPI processes running. \n");
 176                 #endif
 177                 m->mothurOut("The window parameter allows you to specify the window size for searching for chimeras, default=50. \n");
 178                 m->mothurOut("The increment parameter allows you to specify how far you move each window while finding chimeric sequences, default=5.\n");
 179                 m->mothurOut("The numwanted parameter allows you to specify how many sequences you would each query sequence compared with, default=15.\n");
 180                 m->mothurOut("The ksize parameter allows you to input kmersize, default is 7, used if search is kmer. \n");
 181                 m->mothurOut("The match parameter allows you to reward matched bases in blast search, default is 5. \n");
 182                 m->mothurOut("The parents parameter allows you to select the number of potential parents to investigate from the numwanted best matches after rating them, default is 3. \n");
 183                 m->mothurOut("The mismatch parameter allows you to penalize mismatched bases in blast search, default is -4. \n");
 184                 m->mothurOut("The divergence parameter allows you to set a cutoff for chimera determination, default is 1.007. \n");
 185                 m->mothurOut("The iters parameter allows you to specify the number of bootstrap iters to do with the chimeraslayer method, default=100.\n");
 186                 m->mothurOut("The minsim parameter allows you to specify a minimum similarity with the parent fragments, default=90. \n");
 187                 m->mothurOut("The mincov parameter allows you to specify minimum coverage by closest matches found in template. Default is 70, meaning 70%. \n");
 188                 m->mothurOut("The minbs parameter allows you to specify minimum bootstrap support for calling a sequence chimeric. Default is 90, meaning 90%. \n");
 189                 m->mothurOut("The minsnp parameter allows you to specify percent of SNPs to sample on each side of breakpoint for computing bootstrap support (default: 100) \n");
 190                 m->mothurOut("The search parameter allows you to specify search method for finding the closest parent. Choices are distance, blast, and kmer, default distance. \n");
 191                 m->mothurOut("The realign parameter allows you to realign the query to the potential parents. Choices are true or false, default false.  \n");
 192                 m->mothurOut("The chimera.slayer command should be in the following format: \n");
 193                 m->mothurOut("chimera.slayer(fasta=yourFastaFile, template=yourTemplate, search=yourSearch) \n");
 194                 m->mothurOut("Example: chimera.slayer(fasta=AD.align, template=core_set_aligned.imputed.fasta, search=kmer) \n");
 195                 m->mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFastaFile).\n\n");
 196         }
 197         catch(exception& e) {
 198                 m->errorOut(e, "ChimeraSlayerCommand", "help");
 199                 exit(1);
 200         }
 201 }
 202
 203 //***************************************************************************************************************
 204
 205 ChimeraSlayerCommand::~ChimeraSlayerCommand(){  /*      do nothing      */      }
 206
 207 //***************************************************************************************************************
 208
 209 int ChimeraSlayerCommand::execute(){
 210         try{
 211
 212                 if (abort == true) { return 0; }
 213
 214                 for (int s = 0; s < fastaFileNames.size(); s++) {
 215
 216                         m->mothurOut("Checking sequences from " + fastaFileNames[s] + " ..." ); m->mothurOutEndLine();
 217
 218                         int start = time(NULL);
 219
 220                         chimera = new ChimeraSlayer(fastaFileNames[s], templatefile, search, ksize, match, mismatch, window, divR, minSimilarity, minCoverage, minBS, minSNP, parents, iters, increment, numwanted, realign);
 221
 222                         string outputFileName = outputDir + m->getRootName(m->getSimpleName(fastaFileNames[s])) + "slayer.chimeras";
 223                         string accnosFileName = outputDir + m->getRootName(m->getSimpleName(fastaFileNames[s]))  + "slayer.accnos";
 224
 225                         if (m->control_pressed) { delete chimera; for (int j = 0; j < outputNames.size(); j++) {        remove(outputNames[j].c_str()); }  return 0;    }
 226
 227                         if (chimera->getUnaligned()) {
 228                                 m->mothurOut("Your template sequences are different lengths, please correct."); m->mothurOutEndLine();
 229                                 delete chimera;
 230                                 return 0;
 231                         }
 232                         templateSeqsLength = chimera->getLength();
 233
 234                 #ifdef USE_MPI
 235                         int pid, end, numSeqsPerProcessor;
 236                                 int tag = 2001;
 237                                 vector<unsigned long int> MPIPos;
 238
 239                                 MPI_Status status;
 240                                 MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
 241                                 MPI_Comm_size(MPI_COMM_WORLD, &processors);
 242
 243                                 MPI_File inMPI;
 244                                 MPI_File outMPI;
 245                                 MPI_File outMPIAccnos;
 246
 247                                 int outMode=MPI_MODE_CREATE|MPI_MODE_WRONLY;
 248                                 int inMode=MPI_MODE_RDONLY;
 249
 250                                 char outFilename[1024];
 251                                 strcpy(outFilename, outputFileName.c_str());
 252
 253                                 char outAccnosFilename[1024];
 254                                 strcpy(outAccnosFilename, accnosFileName.c_str());
 255
 256                                 char inFileName[1024];
 257                                 strcpy(inFileName, fastaFileNames[s].c_str());
 258
 259                                 MPI_File_open(MPI_COMM_WORLD, inFileName, inMode, MPI_INFO_NULL, &inMPI);  //comm, filename, mode, info, filepointer
 260                                 MPI_File_open(MPI_COMM_WORLD, outFilename, outMode, MPI_INFO_NULL, &outMPI);
 261                                 MPI_File_open(MPI_COMM_WORLD, outAccnosFilename, outMode, MPI_INFO_NULL, &outMPIAccnos);
 262
 263                                 if (m->control_pressed) {  MPI_File_close(&inMPI);  MPI_File_close(&outMPI);   MPI_File_close(&outMPIAccnos); for (int j = 0; j < outputNames.size(); j++) {    remove(outputNames[j].c_str()); }   delete chimera; return 0;  }
 264
 265                                 if (pid == 0) { //you are the root process
 266                                         m->mothurOutEndLine();
 267                                         m->mothurOut("Only reporting sequence supported by " + toString(minBS) + "% of bootstrapped results.");
 268                                         m->mothurOutEndLine();
 269
 270                                         string outTemp = "Name\tLeftParent\tRightParent\tDivQLAQRB\tPerIDQLAQRB\tBootStrapA\tDivQLBQRA\tPerIDQLBQRA\tBootStrapB\tFlag\tLeftWindow\tRightWindow\n";
 271
 272                                         //print header
 273                                         int length = outTemp.length();
 274                                         char* buf2 = new char[length];
 275                                         memcpy(buf2, outTemp.c_str(), length);
 276
 277                                         MPI_File_write_shared(outMPI, buf2, length, MPI_CHAR, &status);
 278                                         delete buf2;
 279
 280                                         MPIPos = m->setFilePosFasta(fastaFileNames[s], numSeqs); //fills MPIPos, returns numSeqs
 281
 282                                         //send file positions to all processes
 283                                         for(int i = 1; i < processors; i++) {
 284                                                 MPI_Send(&numSeqs, 1, MPI_INT, i, tag, MPI_COMM_WORLD);
 285                                                 MPI_Send(&MPIPos[0], (numSeqs+1), MPI_LONG, i, tag, MPI_COMM_WORLD);
 286                                         }
 287
 288                                         //figure out how many sequences you have to align
 289                                         numSeqsPerProcessor = numSeqs / processors;
 290                                         int startIndex =  pid * numSeqsPerProcessor;
 291                                         if(pid == (processors - 1)){    numSeqsPerProcessor = numSeqs - pid * numSeqsPerProcessor;      }
 292
 293                                         //do your part
 294                                         driverMPI(startIndex, numSeqsPerProcessor, inMPI, outMPI, outMPIAccnos, MPIPos);
 295
 296                                         if (m->control_pressed) {  MPI_File_close(&inMPI);  MPI_File_close(&outMPI);   MPI_File_close(&outMPIAccnos);  for (int j = 0; j < outputNames.size(); j++) {   remove(outputNames[j].c_str()); }  remove(outputFileName.c_str());  remove(accnosFileName.c_str());  delete chimera; return 0;  }
 297
 298                                 }else{ //you are a child process
 299                                         MPI_Recv(&numSeqs, 1, MPI_INT, 0, tag, MPI_COMM_WORLD, &status);
 300                                         MPIPos.resize(numSeqs+1);
 301                                         MPI_Recv(&MPIPos[0], (numSeqs+1), MPI_LONG, 0, tag, MPI_COMM_WORLD, &status);
 302
 303                                         //figure out how many sequences you have to align
 304                                         numSeqsPerProcessor = numSeqs / processors;
 305                                         int startIndex =  pid * numSeqsPerProcessor;
 306                                         if(pid == (processors - 1)){    numSeqsPerProcessor = numSeqs - pid * numSeqsPerProcessor;      }
 307
 308                                         //do your part
 309                                         driverMPI(startIndex, numSeqsPerProcessor, inMPI, outMPI, outMPIAccnos, MPIPos);
 310
 311                                         if (m->control_pressed) {  MPI_File_close(&inMPI);  MPI_File_close(&outMPI);   MPI_File_close(&outMPIAccnos);  for (int j = 0; j < outputNames.size(); j++) {   remove(outputNames[j].c_str()); }  delete chimera; return 0;  }
 312                                 }
 313
 314                                 //close files
 315                                 MPI_File_close(&inMPI);
 316                                 MPI_File_close(&outMPI);
 317                                 MPI_File_close(&outMPIAccnos);
 318                                 MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case
 319
 320                 #else
 321                         ofstream outHeader;
 322                         string tempHeader = outputDir + m->getRootName(m->getSimpleName(fastaFileNames[s])) + "slayer.chimeras.tempHeader";
 323                         m->openOutputFile(tempHeader, outHeader);
 324
 325                         chimera->printHeader(outHeader);
 326                         outHeader.close();
 327
 328                         vector<unsigned long int> positions = m->divideFile(fastaFileNames[s], processors);
 329
 330                         for (int i = 0; i < (positions.size()-1); i++) {
 331                                 lines.push_back(new linePair(positions[i], positions[(i+1)]));
 332                         }
 333
 334                         //break up file
 335                         #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
 336                                 if(processors == 1){
 337                                         numSeqs = driver(lines[0], outputFileName, fastaFileNames[s], accnosFileName);
 338
 339                                         if (m->control_pressed) { remove(outputFileName.c_str()); remove(tempHeader.c_str()); remove(accnosFileName.c_str()); for (int j = 0; j < outputNames.size(); j++) {    remove(outputNames[j].c_str()); } for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear(); delete chimera; return 0; }
 340
 341                                 }else{
 342                                         processIDS.resize(0);
 343
 344                                         numSeqs = createProcesses(outputFileName, fastaFileNames[s], accnosFileName);
 345
 346                                         rename((outputFileName + toString(processIDS[0]) + ".temp").c_str(), outputFileName.c_str());
 347                                         rename((accnosFileName + toString(processIDS[0]) + ".temp").c_str(), accnosFileName.c_str());
 348
 349                                         //append output files
 350                                         for(int i=1;i<processors;i++){
 351                                                 m->appendFiles((outputFileName + toString(processIDS[i]) + ".temp"), outputFileName);
 352                                                 remove((outputFileName + toString(processIDS[i]) + ".temp").c_str());
 353                                         }
 354
 355                                         //append output files
 356                                         for(int i=1;i<processors;i++){
 357                                                 m->appendFiles((accnosFileName + toString(processIDS[i]) + ".temp"), accnosFileName);
 358                                                 remove((accnosFileName + toString(processIDS[i]) + ".temp").c_str());
 359                                         }
 360
 361                                         if (m->control_pressed) { remove(outputFileName.c_str()); remove(accnosFileName.c_str()); for (int j = 0; j < outputNames.size(); j++) {        remove(outputNames[j].c_str()); } for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear(); delete chimera; return 0; }
 362                                 }
 363
 364                         #else
 365                                 numSeqs = driver(lines[0], outputFileName, fastaFileNames[s], accnosFileName);
 366
 367                                 if (m->control_pressed) { remove(outputFileName.c_str()); remove(tempHeader.c_str()); remove(accnosFileName.c_str()); for (int j = 0; j < outputNames.size(); j++) {    remove(outputNames[j].c_str()); } for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear(); delete chimera; return 0; }
 368
 369                         #endif
 370
 371                         m->appendFiles(outputFileName, tempHeader);
 372
 373                         remove(outputFileName.c_str());
 374                         rename(tempHeader.c_str(), outputFileName.c_str());
 375
 376                 #endif
 377                         delete chimera;
 378
 379
 380                         for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear();
 381
 382                         outputNames.push_back(outputFileName);
 383                         outputNames.push_back(accnosFileName);
 384
 385                         m->mothurOutEndLine(); m->mothurOut("It took " + toString(time(NULL) - start) + " secs to check " + toString(numSeqs) + " sequences."); m->mothurOutEndLine();
 386                 }
 387
 388                 m->mothurOutEndLine();
 389                 m->mothurOut("Output File Names: "); m->mothurOutEndLine();
 390                 for (int i = 0; i < outputNames.size(); i++) {  m->mothurOut(outputNames[i]); m->mothurOutEndLine();    }
 391                 m->mothurOutEndLine();
 392
 393                 return 0;
 394
 395         }
 396         catch(exception& e) {
 397                 m->errorOut(e, "ChimeraSlayerCommand", "execute");
 398                 exit(1);
 399         }
 400 }
 401 //**********************************************************************************************************************
 402
 403 int ChimeraSlayerCommand::driver(linePair* filePos, string outputFName, string filename, string accnos){
 404         try {
 405                 ofstream out;
 406                 m->openOutputFile(outputFName, out);
 407
 408                 ofstream out2;
 409                 m->openOutputFile(accnos, out2);
 410
 411                 ifstream inFASTA;
 412                 m->openInputFile(filename, inFASTA);
 413
 414                 inFASTA.seekg(filePos->start);
 415
 416                 bool done = false;
 417                 int count = 0;
 418
 419                 while (!done) {
 420
 421                         if (m->control_pressed) {       return 1;       }
 422
 423                         Sequence* candidateSeq = new Sequence(inFASTA);  m->gobble(inFASTA);
 424
 425                         if (candidateSeq->getName() != "") { //incase there is a commented sequence at the end of a file
 426
 427                                 if (candidateSeq->getAligned().length() != templateSeqsLength) {
 428                                         m->mothurOut(candidateSeq->getName() + " is not the same length as the template sequences. Skipping."); m->mothurOutEndLine();
 429                                 }else{
 430                                         //find chimeras
 431                                         chimera->getChimeras(candidateSeq);
 432
 433                                         if (m->control_pressed) {       delete candidateSeq; return 1;  }
 434
 435                                         //print results
 436                                         chimera->print(out, out2);
 437                                 }
 438                         count++;
 439                         }
 440                         delete candidateSeq;
 441
 442                         unsigned long int pos = inFASTA.tellg();
 443                         if ((pos == -1) || (pos >= filePos->end)) { break; }
 444
 445                         //report progress
 446                         if((count) % 100 == 0){ m->mothurOut("Processing sequence: " + toString(count)); m->mothurOutEndLine();         }
 447                 }
 448                 //report progress
 449                 if((count) % 100 != 0){ m->mothurOut("Processing sequence: " + toString(count)); m->mothurOutEndLine();         }
 450
 451                 out.close();
 452                 out2.close();
 453                 inFASTA.close();
 454
 455                 return count;
 456         }
 457         catch(exception& e) {
 458                 m->errorOut(e, "ChimeraSlayerCommand", "driver");
 459                 exit(1);
 460         }
 461 }
 462 //**********************************************************************************************************************
 463 #ifdef USE_MPI
 464 int ChimeraSlayerCommand::driverMPI(int start, int num, MPI_File& inMPI, MPI_File& outMPI, MPI_File& outAccMPI, vector<unsigned long int>& MPIPos){
 465         try {
 466                 MPI_Status status;
 467                 int pid;
 468                 MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
 469
 470                 for(int i=0;i<num;i++){
 471
 472                         if (m->control_pressed) {       return 1;       }
 473
 474                         //read next sequence
 475                         int length = MPIPos[start+i+1] - MPIPos[start+i];
 476
 477                         char* buf4 = new char[length];
 478                         MPI_File_read_at(inMPI, MPIPos[start+i], buf4, length, MPI_CHAR, &status);
 479
 480                         string tempBuf = buf4;
 481                         if (tempBuf.length() > length) { tempBuf = tempBuf.substr(0, length);  }
 482                         istringstream iss (tempBuf,istringstream::in);
 483
 484                         delete buf4;
 485
 486                         Sequence* candidateSeq = new Sequence(iss);  m->gobble(iss);
 487
 488                         if (candidateSeq->getName() != "") { //incase there is a commented sequence at the end of a file
 489
 490                                 if (candidateSeq->getAligned().length() != templateSeqsLength) {
 491                                         m->mothurOut(candidateSeq->getName() + " is not the same length as the template sequences. Skipping."); m->mothurOutEndLine();
 492                                 }else{
 493
 494                                         //find chimeras
 495                                         chimera->getChimeras(candidateSeq);
 496
 497                                         if (m->control_pressed) {       delete candidateSeq; return 1;  }
 498                 //cout << "about to print" << endl;
 499                                         //print results
 500                                         bool isChimeric = chimera->print(outMPI, outAccMPI);
 501                                 }
 502                         }
 503                         delete candidateSeq;
 504
 505                         //report progress
 506                         if((i+1) % 100 == 0){  cout << "Processing sequence: " << (i+1) << endl;        m->mothurOutJustToLog("Processing sequence: " + toString(i+1) + "\n");          }
 507                 }
 508                 //report progress
 509                 if(num % 100 != 0){             cout << "Processing sequence: " << num << endl; m->mothurOutJustToLog("Processing sequence: " + toString(num) + "\n");  }
 510
 511
 512                 return 0;
 513         }
 514         catch(exception& e) {
 515                 m->errorOut(e, "ChimeraSlayerCommand", "driverMPI");
 516                 exit(1);
 517         }
 518 }
 519 #endif
 520
 521 /**************************************************************************************************/
 522
 523 int ChimeraSlayerCommand::createProcesses(string outputFileName, string filename, string accnos) {
 524         try {
 525 #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
 526                 int process = 0;
 527                 int num = 0;
 528
 529                 //loop through and create all the processes you want
 530                 while (process != processors) {
 531                         int pid = fork();
 532
 533                         if (pid > 0) {
 534                                 processIDS.push_back(pid);  //create map from line number to pid so you can append files in correct order later
 535                                 process++;
 536                         }else if (pid == 0){
 537                                 num = driver(lines[process], outputFileName + toString(getpid()) + ".temp", filename, accnos + toString(getpid()) + ".temp");
 538
 539                                 //pass numSeqs to parent
 540                                 ofstream out;
 541                                 string tempFile = outputFileName + toString(getpid()) + ".num.temp";
 542                                 m->openOutputFile(tempFile, out);
 543                                 out << num << endl;
 544                                 out.close();
 545
 546                                 exit(0);
 547                         }else { m->mothurOut("unable to spawn the necessary processes."); m->mothurOutEndLine(); exit(0); }
 548                 }
 549
 550                 //force parent to wait until all the processes are done
 551                 for (int i=0;i<processors;i++) {
 552                         int temp = processIDS[i];
 553                         wait(&temp);
 554                 }
 555
 556                 for (int i = 0; i < processIDS.size(); i++) {
 557                         ifstream in;
 558                         string tempFile =  outputFileName + toString(processIDS[i]) + ".num.temp";
 559                         m->openInputFile(tempFile, in);
 560                         if (!in.eof()) { int tempNum = 0; in >> tempNum; num += tempNum; }
 561                         in.close(); remove(tempFile.c_str());
 562                 }
 563
 564                 return num;
 565 #endif
 566         }
 567         catch(exception& e) {
 568                 m->errorOut(e, "ChimeraSlayerCommand", "createProcesses");
 569                 exit(1);
 570         }
 571 }
 572
 573 /**************************************************************************************************/
 574
 575