chimeraslayercommand.cpp

   1 /*
   2  *  chimeraslayercommand.cpp
   3  *  Mothur
   4  *
   5  *  Created by westcott on 3/31/10.
   6  *  Copyright 2010 Schloss Lab. All rights reserved.
   7  *
   8  */
   9
  10 #include "chimeraslayercommand.h"
  11 #include "chimeraslayer.h"
  12 #include "deconvolutecommand.h"
  13
  14 //**********************************************************************************************************************
  15 vector<string> ChimeraSlayerCommand::setParameters(){
  16         try {
  17                 CommandParameter ptemplate("reference", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(ptemplate);
  18                 CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(pfasta);
  19                 CommandParameter pname("name", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(pname);
  20                 CommandParameter pwindow("window", "Number", "", "50", "", "", "",false,false); parameters.push_back(pwindow);
  21                 CommandParameter pksize("ksize", "Number", "", "7", "", "", "",false,false); parameters.push_back(pksize);
  22                 CommandParameter pmatch("match", "Number", "", "5.0", "", "", "",false,false); parameters.push_back(pmatch);
  23                 CommandParameter pmismatch("mismatch", "Number", "", "-4.0", "", "", "",false,false); parameters.push_back(pmismatch);
  24                 CommandParameter pminsim("minsim", "Number", "", "90", "", "", "",false,false); parameters.push_back(pminsim);
  25                 CommandParameter pmincov("mincov", "Number", "", "70", "", "", "",false,false); parameters.push_back(pmincov);
  26                 CommandParameter pminsnp("minsnp", "Number", "", "10", "", "", "",false,false); parameters.push_back(pminsnp);
  27                 CommandParameter pminbs("minbs", "Number", "", "90", "", "", "",false,false); parameters.push_back(pminbs);
  28                 CommandParameter psearch("search", "Multiple", "kmer-blast", "blast", "", "", "",false,false); parameters.push_back(psearch);
  29                 CommandParameter pprocessors("processors", "Number", "", "1", "", "", "",false,false); parameters.push_back(pprocessors);
  30                 CommandParameter prealign("realign", "Boolean", "", "T", "", "", "",false,false); parameters.push_back(prealign);
  31                 CommandParameter ptrim("trim", "Boolean", "", "F", "", "", "",false,false); parameters.push_back(ptrim);
  32                 CommandParameter psplit("split", "Boolean", "", "F", "", "", "",false,false); parameters.push_back(psplit);
  33                 CommandParameter pnumwanted("numwanted", "Number", "", "15", "", "", "",false,false); parameters.push_back(pnumwanted);
  34                 CommandParameter piters("iters", "Number", "", "1000", "", "", "",false,false); parameters.push_back(piters);
  35                 CommandParameter pdivergence("divergence", "Number", "", "1.007", "", "", "",false,false); parameters.push_back(pdivergence);
  36                 CommandParameter pparents("parents", "Number", "", "3", "", "", "",false,false); parameters.push_back(pparents);
  37                 CommandParameter pincrement("increment", "Number", "", "5", "", "", "",false,false); parameters.push_back(pincrement);
  38                 CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
  39                 CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir);
  40
  41                 vector<string> myArray;
  42                 for (int i = 0; i < parameters.size(); i++) {   myArray.push_back(parameters[i].name);          }
  43                 return myArray;
  44         }
  45         catch(exception& e) {
  46                 m->errorOut(e, "ChimeraSlayerCommand", "setParameters");
  47                 exit(1);
  48         }
  49 }
  50 //**********************************************************************************************************************
  51 string ChimeraSlayerCommand::getHelpString(){
  52         try {
  53                 string helpString = "";
  54                 helpString += "The chimera.slayer command reads a fastafile and referencefile and outputs potentially chimeric sequences.\n";
  55                 helpString += "This command was modeled after the chimeraSlayer written by the Broad Institute.\n";
  56                 helpString += "The chimera.slayer command parameters are fasta, name, template, processors, trim, ksize, window, match, mismatch, divergence. minsim, mincov, minbs, minsnp, parents, search, iters, increment, numwanted, and realign.\n";
  57                 helpString += "The fasta parameter allows you to enter the fasta file containing your potentially chimeric sequences, and is required, unless you have a valid current fasta file. \n";
  58                 helpString += "The name parameter allows you to provide a name file, if you are using template=self. \n";
  59                 helpString += "You may enter multiple fasta files by separating their names with dashes. ie. fasta=abrecovery.fasta-amazon.fasta \n";
  60                 helpString += "The reference parameter allows you to enter a reference file containing known non-chimeric sequences, and is required. You may also set template=self, in this case the abundant sequences will be used as potential parents. \n";
  61                 helpString += "The processors parameter allows you to specify how many processors you would like to use.  The default is 1. \n";
  62 #ifdef USE_MPI
  63                 helpString += "When using MPI, the processors parameter is set to the number of MPI processes running. \n";
  64 #endif
  65                 helpString += "The trim parameter allows you to output a new fasta file containing your sequences with the chimeric ones trimmed to include only their longest piece, default=F. \n";
  66                 helpString += "The split parameter allows you to check both pieces of non-chimeric sequence for chimeras, thus looking for trimeras and quadmeras. default=F. \n";
  67                 helpString += "The window parameter allows you to specify the window size for searching for chimeras, default=50. \n";
  68                 helpString += "The increment parameter allows you to specify how far you move each window while finding chimeric sequences, default=5.\n";
  69                 helpString += "The numwanted parameter allows you to specify how many sequences you would each query sequence compared with, default=15.\n";
  70                 helpString += "The ksize parameter allows you to input kmersize, default is 7, used if search is kmer. \n";
  71                 helpString += "The match parameter allows you to reward matched bases in blast search, default is 5. \n";
  72                 helpString += "The parents parameter allows you to select the number of potential parents to investigate from the numwanted best matches after rating them, default is 3. \n";
  73                 helpString += "The mismatch parameter allows you to penalize mismatched bases in blast search, default is -4. \n";
  74                 helpString += "The divergence parameter allows you to set a cutoff for chimera determination, default is 1.007. \n";
  75                 helpString += "The iters parameter allows you to specify the number of bootstrap iters to do with the chimeraslayer method, default=1000.\n";
  76                 helpString += "The minsim parameter allows you to specify a minimum similarity with the parent fragments, default=90. \n";
  77                 helpString += "The mincov parameter allows you to specify minimum coverage by closest matches found in template. Default is 70, meaning 70%. \n";
  78                 helpString += "The minbs parameter allows you to specify minimum bootstrap support for calling a sequence chimeric. Default is 90, meaning 90%. \n";
  79                 helpString += "The minsnp parameter allows you to specify percent of SNPs to sample on each side of breakpoint for computing bootstrap support (default: 10) \n";
  80                 helpString += "The search parameter allows you to specify search method for finding the closest parent. Choices are blast, and kmer, default blast. \n";
  81                 helpString += "The realign parameter allows you to realign the query to the potential parents. Choices are true or false, default true.  \n";
  82                 helpString += "The chimera.slayer command should be in the following format: \n";
  83                 helpString += "chimera.slayer(fasta=yourFastaFile, reference=yourTemplate, search=yourSearch) \n";
  84                 helpString += "Example: chimera.slayer(fasta=AD.align, reference=core_set_aligned.imputed.fasta, search=kmer) \n";
  85                 helpString += "Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFastaFile).\n";
  86                 return helpString;
  87         }
  88         catch(exception& e) {
  89                 m->errorOut(e, "ChimeraSlayerCommand", "getHelpString");
  90                 exit(1);
  91         }
  92 }
  93 //**********************************************************************************************************************
  94 ChimeraSlayerCommand::ChimeraSlayerCommand(){
  95         try {
  96                 abort = true; calledHelp = true;
  97                 setParameters();
  98                 vector<string> tempOutNames;
  99                 outputTypes["chimera"] = tempOutNames;
 100                 outputTypes["accnos"] = tempOutNames;
 101                 outputTypes["fasta"] = tempOutNames;
 102         }
 103         catch(exception& e) {
 104                 m->errorOut(e, "ChimeraSlayerCommand", "ChimeraSlayerCommand");
 105                 exit(1);
 106         }
 107 }
 108 //***************************************************************************************************************
 109 ChimeraSlayerCommand::ChimeraSlayerCommand(string option)  {
 110         try {
 111                 abort = false; calledHelp = false;
 112
 113                 //allow user to run help
 114                 if(option == "help") { help(); abort = true; calledHelp = true; }
 115                 else if(option == "citation") { citation(); abort = true; calledHelp = true;}
 116
 117                 else {
 118                         vector<string> myArray = setParameters();
 119
 120                         OptionParser parser(option);
 121                         map<string,string> parameters = parser.getParameters();
 122
 123                         ValidParameters validParameter("chimera.slayer");
 124                         map<string,string>::iterator it;
 125
 126                         //check to make sure all parameters are valid for command
 127                         for (it = parameters.begin(); it != parameters.end(); it++) {
 128                                 if (validParameter.isValidParameter(it->first, myArray, it->second) != true) {  abort = true;  }
 129                         }
 130
 131                         vector<string> tempOutNames;
 132                         outputTypes["chimera"] = tempOutNames;
 133                         outputTypes["accnos"] = tempOutNames;
 134                         outputTypes["fasta"] = tempOutNames;
 135
 136                         //if the user changes the input directory command factory will send this info to us in the output parameter
 137                         string inputDir = validParameter.validFile(parameters, "inputdir", false);
 138                         if (inputDir == "not found"){   inputDir = "";          }
 139
 140                         //check for required parameters
 141                         fastafile = validParameter.validFile(parameters, "fasta", false);
 142                         if (fastafile == "not found") {
 143                                 //if there is a current fasta file, use it
 144                                 string filename = m->getFastaFile();
 145                                 if (filename != "") { fastaFileNames.push_back(filename); m->mothurOut("Using " + filename + " as input file for the fasta parameter."); m->mothurOutEndLine(); }
 146                                 else {  m->mothurOut("You have no current fastafile and the fasta parameter is required."); m->mothurOutEndLine(); abort = true; }
 147                         }else {
 148                                 m->splitAtDash(fastafile, fastaFileNames);
 149
 150                                 //go through files and make sure they are good, if not, then disregard them
 151                                 for (int i = 0; i < fastaFileNames.size(); i++) {
 152
 153                                         bool ignore = false;
 154                                         if (fastaFileNames[i] == "current") {
 155                                                 fastaFileNames[i] = m->getFastaFile();
 156                                                 if (fastaFileNames[i] != "") {  m->mothurOut("Using " + fastaFileNames[i] + " as input file for the fasta parameter where you had given current."); m->mothurOutEndLine(); }
 157                                                 else {
 158                                                         m->mothurOut("You have no current fastafile, ignoring current."); m->mothurOutEndLine(); ignore=true;
 159                                                         //erase from file list
 160                                                         fastaFileNames.erase(fastaFileNames.begin()+i);
 161                                                         i--;
 162                                                 }
 163                                         }
 164
 165                                         if (!ignore) {
 166
 167                                                 if (inputDir != "") {
 168                                                         string path = m->hasPath(fastaFileNames[i]);
 169                                                         //if the user has not given a path then, add inputdir. else leave path alone.
 170                                                         if (path == "") {       fastaFileNames[i] = inputDir + fastaFileNames[i];               }
 171                                                 }
 172
 173                                                 int ableToOpen;
 174                                                 ifstream in;
 175
 176                                                 ableToOpen = m->openInputFile(fastaFileNames[i], in, "noerror");
 177
 178                                                 //if you can't open it, try default location
 179                                                 if (ableToOpen == 1) {
 180                                                         if (m->getDefaultPath() != "") { //default path is set
 181                                                                 string tryPath = m->getDefaultPath() + m->getSimpleName(fastaFileNames[i]);
 182                                                                 m->mothurOut("Unable to open " + fastaFileNames[i] + ". Trying default " + tryPath); m->mothurOutEndLine();
 183                                                                 ifstream in2;
 184                                                                 ableToOpen = m->openInputFile(tryPath, in2, "noerror");
 185                                                                 in2.close();
 186                                                                 fastaFileNames[i] = tryPath;
 187                                                         }
 188                                                 }
 189
 190                                                 if (ableToOpen == 1) {
 191                                                         if (m->getOutputDir() != "") { //default path is set
 192                                                                 string tryPath = m->getOutputDir() + m->getSimpleName(fastaFileNames[i]);
 193                                                                 m->mothurOut("Unable to open " + fastaFileNames[i] + ". Trying output directory " + tryPath); m->mothurOutEndLine();
 194                                                                 ifstream in2;
 195                                                                 ableToOpen = m->openInputFile(tryPath, in2, "noerror");
 196                                                                 in2.close();
 197                                                                 fastaFileNames[i] = tryPath;
 198                                                         }
 199                                                 }
 200
 201                                                 in.close();
 202
 203                                                 if (ableToOpen == 1) {
 204                                                         m->mothurOut("Unable to open " + fastaFileNames[i] + ". It will be disregarded."); m->mothurOutEndLine();
 205                                                         //erase from file list
 206                                                         fastaFileNames.erase(fastaFileNames.begin()+i);
 207                                                         i--;
 208                                                 }else {
 209                                                         m->setFastaFile(fastaFileNames[i]);
 210                                                 }
 211                                         }
 212                                 }
 213
 214                                 //make sure there is at least one valid file left
 215                                 if (fastaFileNames.size() == 0) { m->mothurOut("[ERROR]: no valid files."); m->mothurOutEndLine(); abort = true; }
 216                         }
 217
 218
 219                         //check for required parameters
 220                         bool hasName = true;
 221                         namefile = validParameter.validFile(parameters, "name", false);
 222                         if (namefile == "not found") { namefile = "";  hasName = false; }
 223                         else {
 224                                 m->splitAtDash(namefile, nameFileNames);
 225
 226                                 //go through files and make sure they are good, if not, then disregard them
 227                                 for (int i = 0; i < nameFileNames.size(); i++) {
 228
 229                                         bool ignore = false;
 230                                         if (nameFileNames[i] == "current") {
 231                                                 nameFileNames[i] = m->getNameFile();
 232                                                 if (nameFileNames[i] != "") {  m->mothurOut("Using " + nameFileNames[i] + " as input file for the name parameter where you had given current."); m->mothurOutEndLine(); }
 233                                                 else {
 234                                                         m->mothurOut("You have no current namefile, ignoring current."); m->mothurOutEndLine(); ignore=true;
 235                                                         //erase from file list
 236                                                         nameFileNames.erase(nameFileNames.begin()+i);
 237                                                         i--;
 238                                                 }
 239                                         }
 240
 241                                         if (!ignore) {
 242
 243                                                 if (inputDir != "") {
 244                                                         string path = m->hasPath(nameFileNames[i]);
 245                                                         //if the user has not given a path then, add inputdir. else leave path alone.
 246                                                         if (path == "") {       nameFileNames[i] = inputDir + nameFileNames[i];         }
 247                                                 }
 248
 249                                                 int ableToOpen;
 250                                                 ifstream in;
 251
 252                                                 ableToOpen = m->openInputFile(nameFileNames[i], in, "noerror");
 253
 254                                                 //if you can't open it, try default location
 255                                                 if (ableToOpen == 1) {
 256                                                         if (m->getDefaultPath() != "") { //default path is set
 257                                                                 string tryPath = m->getDefaultPath() + m->getSimpleName(nameFileNames[i]);
 258                                                                 m->mothurOut("Unable to open " + nameFileNames[i] + ". Trying default " + tryPath); m->mothurOutEndLine();
 259                                                                 ifstream in2;
 260                                                                 ableToOpen = m->openInputFile(tryPath, in2, "noerror");
 261                                                                 in2.close();
 262                                                                 nameFileNames[i] = tryPath;
 263                                                         }
 264                                                 }
 265
 266                                                 if (ableToOpen == 1) {
 267                                                         if (m->getOutputDir() != "") { //default path is set
 268                                                                 string tryPath = m->getOutputDir() + m->getSimpleName(nameFileNames[i]);
 269                                                                 m->mothurOut("Unable to open " + nameFileNames[i] + ". Trying output directory " + tryPath); m->mothurOutEndLine();
 270                                                                 ifstream in2;
 271                                                                 ableToOpen = m->openInputFile(tryPath, in2, "noerror");
 272                                                                 in2.close();
 273                                                                 nameFileNames[i] = tryPath;
 274                                                         }
 275                                                 }
 276
 277                                                 in.close();
 278
 279                                                 if (ableToOpen == 1) {
 280                                                         m->mothurOut("Unable to open " + nameFileNames[i] + ". It will be disregarded."); m->mothurOutEndLine();
 281                                                         //erase from file list
 282                                                         nameFileNames.erase(nameFileNames.begin()+i);
 283                                                         i--;
 284                                                 }else {
 285                                                         m->setNameFile(nameFileNames[i]);
 286                                                 }
 287                                         }
 288                                 }
 289
 290                                 //make sure there is at least one valid file left
 291                                 if (nameFileNames.size() == 0) { m->mothurOut("[ERROR]: no valid name files."); m->mothurOutEndLine(); abort = true; }
 292                         }
 293
 294                         if (hasName && (nameFileNames.size() != fastaFileNames.size())) { m->mothurOut("[ERROR]: The number of namefiles does not match the number of fastafiles, please correct."); m->mothurOutEndLine(); abort=true; }
 295
 296                         //if the user changes the output directory command factory will send this info to us in the output parameter
 297                         outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){  outputDir = ""; }
 298
 299
 300                         string path;
 301                         it = parameters.find("reference");
 302                         //user has given a template file
 303                         if(it != parameters.end()){
 304                                 if (it->second == "self") { templatefile = "self"; }
 305                                 else {
 306                                         path = m->hasPath(it->second);
 307                                         //if the user has not given a path then, add inputdir. else leave path alone.
 308                                         if (path == "") {       parameters["reference"] = inputDir + it->second;                }
 309
 310                                         templatefile = validParameter.validFile(parameters, "reference", true);
 311                                         if (templatefile == "not open") { abort = true; }
 312                                         else if (templatefile == "not found") { templatefile = "";  m->mothurOut("reference is a required parameter for the chimera.slayer command, unless and namefile is given."); m->mothurOutEndLine(); abort = true;  }
 313                                 }
 314                         }else if (hasName) {  templatefile = "self"; }
 315                         else { templatefile = "";  m->mothurOut("reference is a required parameter for the chimera.slayer command."); m->mothurOutEndLine(); abort = true;  }
 316
 317                         string temp = validParameter.validFile(parameters, "processors", false);        if (temp == "not found"){       temp = m->getProcessors();      }
 318                         m->setProcessors(temp);
 319                         convert(temp, processors);
 320
 321                         temp = validParameter.validFile(parameters, "ksize", false);                    if (temp == "not found") { temp = "7"; }
 322                         convert(temp, ksize);
 323
 324                         temp = validParameter.validFile(parameters, "window", false);                   if (temp == "not found") { temp = "50"; }
 325                         convert(temp, window);
 326
 327                         temp = validParameter.validFile(parameters, "match", false);                    if (temp == "not found") { temp = "5"; }
 328                         convert(temp, match);
 329
 330                         temp = validParameter.validFile(parameters, "mismatch", false);                 if (temp == "not found") { temp = "-4"; }
 331                         convert(temp, mismatch);
 332
 333                         temp = validParameter.validFile(parameters, "divergence", false);               if (temp == "not found") { temp = "1.007"; }
 334                         convert(temp, divR);
 335
 336                         temp = validParameter.validFile(parameters, "minsim", false);                   if (temp == "not found") { temp = "90"; }
 337                         convert(temp, minSimilarity);
 338
 339                         temp = validParameter.validFile(parameters, "mincov", false);                   if (temp == "not found") { temp = "70"; }
 340                         convert(temp, minCoverage);
 341
 342                         temp = validParameter.validFile(parameters, "minbs", false);                    if (temp == "not found") { temp = "90"; }
 343                         convert(temp, minBS);
 344
 345                         temp = validParameter.validFile(parameters, "minsnp", false);                   if (temp == "not found") { temp = "10"; }
 346                         convert(temp, minSNP);
 347
 348                         temp = validParameter.validFile(parameters, "parents", false);                  if (temp == "not found") { temp = "3"; }
 349                         convert(temp, parents);
 350
 351                         temp = validParameter.validFile(parameters, "realign", false);                  if (temp == "not found") { temp = "t"; }
 352                         realign = m->isTrue(temp);
 353
 354                         temp = validParameter.validFile(parameters, "trim", false);                             if (temp == "not found") { temp = "f"; }
 355                         trim = m->isTrue(temp);
 356
 357                         temp = validParameter.validFile(parameters, "split", false);                    if (temp == "not found") { temp = "f"; }
 358                         trimera = m->isTrue(temp);
 359
 360                         search = validParameter.validFile(parameters, "search", false);                 if (search == "not found") { search = "blast"; }
 361
 362                         temp = validParameter.validFile(parameters, "iters", false);                    if (temp == "not found") { temp = "1000"; }
 363                         convert(temp, iters);
 364
 365                         temp = validParameter.validFile(parameters, "increment", false);                if (temp == "not found") { temp = "5"; }
 366                         convert(temp, increment);
 367
 368                         temp = validParameter.validFile(parameters, "numwanted", false);                if (temp == "not found") { temp = "15"; }
 369                         convert(temp, numwanted);
 370
 371                         if ((search != "blast") && (search != "kmer")) { m->mothurOut(search + " is not a valid search."); m->mothurOutEndLine(); abort = true;  }
 372                 }
 373         }
 374         catch(exception& e) {
 375                 m->errorOut(e, "ChimeraSlayerCommand", "ChimeraSlayerCommand");
 376                 exit(1);
 377         }
 378 }
 379 //***************************************************************************************************************
 380
 381 int ChimeraSlayerCommand::execute(){
 382         try{
 383                 if (abort == true) { if (calledHelp) { return 0; }  return 2;   }
 384
 385                 for (int s = 0; s < fastaFileNames.size(); s++) {
 386
 387                         m->mothurOut("Checking sequences from " + fastaFileNames[s] + " ..." ); m->mothurOutEndLine();
 388
 389                         int start = time(NULL);
 390
 391                         if (templatefile != "self") { //you want to run slayer with a refernce template
 392                                 chimera = new ChimeraSlayer(fastaFileNames[s], templatefile, trim, search, ksize, match, mismatch, window, divR, minSimilarity, minCoverage, minBS, minSNP, parents, iters, increment, numwanted, realign);
 393                         }else {
 394                                 if (processors != 1) { m->mothurOut("When using template=self, mothur can only use 1 processor, continuing."); m->mothurOutEndLine(); processors = 1; }
 395                                 string nameFile = "";
 396                                 if (nameFileNames.size() != 0) { //you provided a namefile and we don't need to create one
 397                                         nameFile = nameFileNames[s];
 398                                 }else {
 399                                         m->mothurOutEndLine(); m->mothurOut("No namesfile given, running unique.seqs command to generate one."); m->mothurOutEndLine(); m->mothurOutEndLine();
 400
 401                                         //use unique.seqs to create new name and fastafile
 402                                         string inputString = "fasta=" + fastaFileNames[s];
 403                                         m->mothurOut("/******************************************/"); m->mothurOutEndLine();
 404                                         m->mothurOut("Running command: unique.seqs(" + inputString + ")"); m->mothurOutEndLine();
 405
 406                                         Command* uniqueCommand = new DeconvoluteCommand(inputString);
 407                                         uniqueCommand->execute();
 408
 409                                         map<string, vector<string> > filenames = uniqueCommand->getOutputFiles();
 410
 411                                         delete uniqueCommand;
 412
 413                                         m->mothurOut("/******************************************/"); m->mothurOutEndLine();
 414
 415                                         nameFile = filenames["name"][0];
 416                                         fastaFileNames[s] = filenames["fasta"][0];
 417                                 }
 418
 419                                 //sort fastafile by abundance, returns new sorted fastafile name
 420                                 m->mothurOut("Sorting fastafile according to abundance..."); cout.flush();
 421                                 map<string, int> priority = sortFastaFile(fastaFileNames[s], nameFile);
 422                                 m->mothurOut("Done."); m->mothurOutEndLine();
 423
 424                                 if (m->control_pressed) {  for (int j = 0; j < outputNames.size(); j++) {       remove(outputNames[j].c_str()); }  return 0;    }
 425
 426                                 chimera = new ChimeraSlayer(fastaFileNames[s], templatefile, trim, priority, search, ksize, match, mismatch, window, divR, minSimilarity, minCoverage, minBS, minSNP, parents, iters, increment, numwanted, realign);
 427                         }
 428
 429                         if (outputDir == "") { outputDir = m->hasPath(fastaFileNames[s]);  }//if user entered a file with a path then preserve it
 430                         string outputFileName = outputDir + m->getRootName(m->getSimpleName(fastaFileNames[s])) + "slayer.chimera";
 431                         string accnosFileName = outputDir + m->getRootName(m->getSimpleName(fastaFileNames[s]))  + "slayer.accnos";
 432                         string trimFastaFileName = outputDir + m->getRootName(m->getSimpleName(fastaFileNames[s]))  + "slayer.fasta";
 433
 434                         if (m->control_pressed) { delete chimera; for (int j = 0; j < outputNames.size(); j++) {        remove(outputNames[j].c_str()); }  return 0;    }
 435
 436                         if (chimera->getUnaligned()) {
 437                                 m->mothurOut("Your template sequences are different lengths, please correct."); m->mothurOutEndLine();
 438                                 delete chimera;
 439                                 return 0;
 440                         }
 441                         templateSeqsLength = chimera->getLength();
 442
 443                 #ifdef USE_MPI
 444                         int pid, numSeqsPerProcessor;
 445                                 int tag = 2001;
 446                                 vector<unsigned long int> MPIPos;
 447
 448                                 MPI_Status status;
 449                                 MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
 450                                 MPI_Comm_size(MPI_COMM_WORLD, &processors);
 451
 452                                 MPI_File inMPI;
 453                                 MPI_File outMPI;
 454                                 MPI_File outMPIAccnos;
 455                                 MPI_File outMPIFasta;
 456
 457                                 int outMode=MPI_MODE_CREATE|MPI_MODE_WRONLY;
 458                                 int inMode=MPI_MODE_RDONLY;
 459
 460                                 char outFilename[1024];
 461                                 strcpy(outFilename, outputFileName.c_str());
 462
 463                                 char outAccnosFilename[1024];
 464                                 strcpy(outAccnosFilename, accnosFileName.c_str());
 465
 466                                 char outFastaFilename[1024];
 467                                 strcpy(outFastaFilename, trimFastaFileName.c_str());
 468
 469                                 char inFileName[1024];
 470                                 strcpy(inFileName, fastaFileNames[s].c_str());
 471
 472                                 MPI_File_open(MPI_COMM_WORLD, inFileName, inMode, MPI_INFO_NULL, &inMPI);  //comm, filename, mode, info, filepointer
 473                                 MPI_File_open(MPI_COMM_WORLD, outFilename, outMode, MPI_INFO_NULL, &outMPI);
 474                                 MPI_File_open(MPI_COMM_WORLD, outAccnosFilename, outMode, MPI_INFO_NULL, &outMPIAccnos);
 475                                 if (trim) { MPI_File_open(MPI_COMM_WORLD, outFastaFilename, outMode, MPI_INFO_NULL, &outMPIFasta); }
 476
 477                         if (m->control_pressed) { outputTypes.clear();  MPI_File_close(&inMPI);  MPI_File_close(&outMPI); if (trim) {  MPI_File_close(&outMPIFasta);  } MPI_File_close(&outMPIAccnos); for (int j = 0; j < outputNames.size(); j++) {   remove(outputNames[j].c_str()); }   delete chimera; return 0;  }
 478
 479                                 if (pid == 0) { //you are the root process
 480                                         m->mothurOutEndLine();
 481                                         m->mothurOut("Only reporting sequence supported by " + toString(minBS) + "% of bootstrapped results.");
 482                                         m->mothurOutEndLine();
 483
 484                                         string outTemp = "Name\tLeftParent\tRightParent\tDivQLAQRB\tPerIDQLAQRB\tBootStrapA\tDivQLBQRA\tPerIDQLBQRA\tBootStrapB\tFlag\tLeftWindow\tRightWindow\n";
 485
 486                                         //print header
 487                                         int length = outTemp.length();
 488                                         char* buf2 = new char[length];
 489                                         memcpy(buf2, outTemp.c_str(), length);
 490
 491                                         MPI_File_write_shared(outMPI, buf2, length, MPI_CHAR, &status);
 492                                         delete buf2;
 493
 494                                         MPIPos = m->setFilePosFasta(fastaFileNames[s], numSeqs); //fills MPIPos, returns numSeqs
 495
 496                                         if (templatefile != "self") { //if template=self we can only use 1 processor
 497                                                 //send file positions to all processes
 498                                                 for(int i = 1; i < processors; i++) {
 499                                                         MPI_Send(&numSeqs, 1, MPI_INT, i, tag, MPI_COMM_WORLD);
 500                                                         MPI_Send(&MPIPos[0], (numSeqs+1), MPI_LONG, i, tag, MPI_COMM_WORLD);
 501                                                 }
 502                                         }
 503                                         //figure out how many sequences you have to align
 504                                         numSeqsPerProcessor = numSeqs / processors;
 505                                         int startIndex =  pid * numSeqsPerProcessor;
 506                                         if(pid == (processors - 1)){    numSeqsPerProcessor = numSeqs - pid * numSeqsPerProcessor;      }
 507
 508                                         if (templatefile == "self") { //if template=self we can only use 1 processor
 509                                                 startIndex = 0;
 510                                                 numSeqsPerProcessor = numSeqs;
 511                                         }
 512
 513                                         //do your part
 514                                         driverMPI(startIndex, numSeqsPerProcessor, inMPI, outMPI, outMPIAccnos, outMPIFasta, MPIPos);
 515
 516                                         int numNoParents = chimera->getNumNoParents();
 517                                         int temp;
 518                                         for(int i = 1; i < processors; i++) {
 519                                                 MPI_Recv(&temp, 1, MPI_INT, 1, tag, MPI_COMM_WORLD, &status);
 520                                                 numNoParents += temp;
 521                                         }
 522
 523
 524                                         if (numSeqs == numNoParents) {  m->mothurOut("[WARNING]: megablast returned 0 potential parents for all your sequences. This could be due to formatdb.exe not being setup properly, please check formatdb.log for errors."); m->mothurOutEndLine(); }
 525
 526                                         if (m->control_pressed) { outputTypes.clear();  MPI_File_close(&inMPI);  MPI_File_close(&outMPI); if (trim) { MPI_File_close(&outMPIFasta); }  MPI_File_close(&outMPIAccnos);  for (int j = 0; j < outputNames.size(); j++) {   remove(outputNames[j].c_str()); }  remove(outputFileName.c_str());  remove(accnosFileName.c_str());  delete chimera; return 0;  }
 527
 528                                 }else{ //you are a child process
 529                                         if (templatefile != "self") { //if template=self we can only use 1 processor
 530                                                 MPI_Recv(&numSeqs, 1, MPI_INT, 0, tag, MPI_COMM_WORLD, &status);
 531                                                 MPIPos.resize(numSeqs+1);
 532                                                 MPI_Recv(&MPIPos[0], (numSeqs+1), MPI_LONG, 0, tag, MPI_COMM_WORLD, &status);
 533
 534                                                 //figure out how many sequences you have to align
 535                                                 numSeqsPerProcessor = numSeqs / processors;
 536                                                 int startIndex =  pid * numSeqsPerProcessor;
 537                                                 if(pid == (processors - 1)){    numSeqsPerProcessor = numSeqs - pid * numSeqsPerProcessor;      }
 538
 539                                                 //do your part
 540                                                 driverMPI(startIndex, numSeqsPerProcessor, inMPI, outMPI, outMPIAccnos, outMPIFasta, MPIPos);
 541
 542                                                 int numNoParents = chimera->getNumNoParents();
 543                                                 MPI_Send(&numNoParents, 1, MPI_INT, 0, tag, MPI_COMM_WORLD);
 544
 545                                                 if (m->control_pressed) { outputTypes.clear();  MPI_File_close(&inMPI);  MPI_File_close(&outMPI); if (trim) { MPI_File_close(&outMPIFasta); }  MPI_File_close(&outMPIAccnos);  for (int j = 0; j < outputNames.size(); j++) {   remove(outputNames[j].c_str()); }  delete chimera; return 0;  }
 546
 547                                         }
 548                                 }
 549
 550                                 //close files
 551                                 MPI_File_close(&inMPI);
 552                                 MPI_File_close(&outMPI);
 553                                 MPI_File_close(&outMPIAccnos);
 554                                 if (trim) { MPI_File_close(&outMPIFasta); }
 555                                 MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case
 556
 557                 #else
 558                         ofstream outHeader;
 559                         string tempHeader = outputDir + m->getRootName(m->getSimpleName(fastaFileNames[s])) + "slayer.chimeras.tempHeader";
 560                         m->openOutputFile(tempHeader, outHeader);
 561
 562                         chimera->printHeader(outHeader);
 563                         outHeader.close();
 564
 565                         vector<unsigned long int> positions = m->divideFile(fastaFileNames[s], processors);
 566
 567                         for (int i = 0; i < (positions.size()-1); i++) {
 568                                 lines.push_back(new linePair(positions[i], positions[(i+1)]));
 569                         }
 570
 571                         //break up file
 572                         #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
 573                                 if(processors == 1){
 574                                         numSeqs = driver(lines[0], outputFileName, fastaFileNames[s], accnosFileName, trimFastaFileName);
 575
 576                                         int numNoParents = chimera->getNumNoParents();
 577                                         if (numNoParents == numSeqs) { m->mothurOut("[WARNING]: megablast returned 0 potential parents for all your sequences. This could be due to formatdb.exe not being setup properly, please check formatdb.log for errors."); m->mothurOutEndLine(); }
 578
 579                                         if (m->control_pressed) { outputTypes.clear(); if (trim) { remove(trimFastaFileName.c_str()); } remove(outputFileName.c_str()); remove(tempHeader.c_str()); remove(accnosFileName.c_str()); for (int j = 0; j < outputNames.size(); j++) {      remove(outputNames[j].c_str()); } for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear(); delete chimera; return 0; }
 580
 581                                 }else{
 582                                         processIDS.resize(0);
 583
 584                                         numSeqs = createProcesses(outputFileName, fastaFileNames[s], accnosFileName, trimFastaFileName);
 585
 586                                         rename((outputFileName + toString(processIDS[0]) + ".temp").c_str(), outputFileName.c_str());
 587                                         rename((accnosFileName + toString(processIDS[0]) + ".temp").c_str(), accnosFileName.c_str());
 588                                         if (trim) {  rename((trimFastaFileName + toString(processIDS[0]) + ".temp").c_str(), trimFastaFileName.c_str()); }
 589
 590                                         //append output files
 591                                         for(int i=1;i<processors;i++){
 592                                                 m->appendFiles((outputFileName + toString(processIDS[i]) + ".temp"), outputFileName);
 593                                                 remove((outputFileName + toString(processIDS[i]) + ".temp").c_str());
 594                                         }
 595
 596                                         //append output files
 597                                         for(int i=1;i<processors;i++){
 598                                                 m->appendFiles((accnosFileName + toString(processIDS[i]) + ".temp"), accnosFileName);
 599                                                 remove((accnosFileName + toString(processIDS[i]) + ".temp").c_str());
 600                                         }
 601
 602                                         if (trim) {
 603                                                 for(int i=1;i<processors;i++){
 604                                                         m->appendFiles((trimFastaFileName + toString(processIDS[i]) + ".temp"), trimFastaFileName);
 605                                                         remove((trimFastaFileName + toString(processIDS[i]) + ".temp").c_str());
 606                                                 }
 607                                         }
 608
 609                                         if (m->control_pressed) { outputTypes.clear(); if (trim) { remove(trimFastaFileName.c_str()); } remove(outputFileName.c_str()); remove(accnosFileName.c_str()); for (int j = 0; j < outputNames.size(); j++) {  remove(outputNames[j].c_str()); } for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear(); delete chimera; return 0; }
 610                                 }
 611
 612                         #else
 613                                 numSeqs = driver(lines[0], outputFileName, fastaFileNames[s], accnosFileName, trimFastaFileName);
 614
 615                                 int numNoParents = chimera->getNumNoParents();
 616                                 if (numNoParents == numSeqs) { m->mothurOut("[WARNING]: megablast returned 0 potential parents for all your sequences. This could be due to formatdb.exe not being setup properly, please check formatdb.log for errors."); m->mothurOutEndLine(); }
 617
 618
 619                                 if (m->control_pressed) { outputTypes.clear(); if (trim) { remove(trimFastaFileName.c_str()); } remove(outputFileName.c_str()); remove(tempHeader.c_str()); remove(accnosFileName.c_str()); for (int j = 0; j < outputNames.size(); j++) {      remove(outputNames[j].c_str()); } for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear(); delete chimera; return 0; }
 620
 621                         #endif
 622
 623                         m->appendFiles(outputFileName, tempHeader);
 624
 625                         remove(outputFileName.c_str());
 626                         rename(tempHeader.c_str(), outputFileName.c_str());
 627
 628                 #endif
 629                         delete chimera;
 630
 631
 632                         for (int i = 0; i < lines.size(); i++) {  delete lines[i];  }  lines.clear();
 633
 634                         outputNames.push_back(outputFileName); outputTypes["chimera"].push_back(outputFileName);
 635                         outputNames.push_back(accnosFileName); outputTypes["accnos"].push_back(accnosFileName);
 636                         if (trim) {  outputNames.push_back(trimFastaFileName); outputTypes["fasta"].push_back(trimFastaFileName); }
 637
 638                         m->mothurOutEndLine(); m->mothurOut("It took " + toString(time(NULL) - start) + " secs to check " + toString(numSeqs) + " sequences."); m->mothurOutEndLine();
 639                 }
 640
 641                 //set accnos file as new current accnosfile
 642                 string current = "";
 643                 itTypes = outputTypes.find("accnos");
 644                 if (itTypes != outputTypes.end()) {
 645                         if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setAccnosFile(current); }
 646                 }
 647
 648                 if (trim) {
 649                         itTypes = outputTypes.find("fasta");
 650                         if (itTypes != outputTypes.end()) {
 651                                 if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setFastaFile(current); }
 652                         }
 653                 }
 654
 655                 m->mothurOutEndLine();
 656                 m->mothurOut("Output File Names: "); m->mothurOutEndLine();
 657                 for (int i = 0; i < outputNames.size(); i++) {  m->mothurOut(outputNames[i]); m->mothurOutEndLine();    }
 658                 m->mothurOutEndLine();
 659
 660                 return 0;
 661
 662         }
 663         catch(exception& e) {
 664                 m->errorOut(e, "ChimeraSlayerCommand", "execute");
 665                 exit(1);
 666         }
 667 }
 668 //**********************************************************************************************************************
 669
 670 int ChimeraSlayerCommand::driver(linePair* filePos, string outputFName, string filename, string accnos, string fasta){
 671         try {
 672                 ofstream out;
 673                 m->openOutputFile(outputFName, out);
 674
 675                 ofstream out2;
 676                 m->openOutputFile(accnos, out2);
 677
 678                 ofstream out3;
 679                 if (trim) {  m->openOutputFile(fasta, out3); }
 680
 681                 ifstream inFASTA;
 682                 m->openInputFile(filename, inFASTA);
 683
 684                 inFASTA.seekg(filePos->start);
 685
 686                 bool done = false;
 687                 int count = 0;
 688
 689                 while (!done) {
 690
 691                         if (m->control_pressed) {       out.close(); out2.close(); if (trim) { out3.close(); } inFASTA.close(); return 1;       }
 692
 693                         Sequence* candidateSeq = new Sequence(inFASTA);  m->gobble(inFASTA);
 694                         string candidateAligned = candidateSeq->getAligned();
 695
 696                         if (candidateSeq->getName() != "") { //incase there is a commented sequence at the end of a file
 697                                 if (candidateSeq->getAligned().length() != templateSeqsLength) {
 698                                         m->mothurOut(candidateSeq->getName() + " is not the same length as the template sequences. Skipping."); m->mothurOutEndLine();
 699                                 }else{
 700                                         //find chimeras
 701                                         chimera->getChimeras(candidateSeq);
 702
 703                                         if (m->control_pressed) {       delete candidateSeq; return 1;  }
 704
 705                                         //if you are not chimeric, then check each half
 706                                         data_results wholeResults = chimera->getResults();
 707
 708                                         //determine if we need to split
 709                                         bool isChimeric = false;
 710
 711                                         if (wholeResults.flag == "yes") {
 712                                                 string chimeraFlag = "no";
 713                                                 if(  (wholeResults.results[0].bsa >= minBS && wholeResults.results[0].divr_qla_qrb >= divR)
 714                                                    ||
 715                                                    (wholeResults.results[0].bsb >= minBS && wholeResults.results[0].divr_qlb_qra >= divR) ) { chimeraFlag = "yes"; }
 716
 717
 718                                                 if (chimeraFlag == "yes") {
 719                                                         if ((wholeResults.results[0].bsa >= minBS) || (wholeResults.results[0].bsb >= minBS)) { isChimeric = true; }
 720                                                 }
 721                                         }
 722
 723                                         if ((!isChimeric) && trimera) {
 724
 725                                                 //split sequence in half by bases
 726                                                 string leftQuery, rightQuery;
 727                                                 Sequence tempSeq(candidateSeq->getName(), candidateAligned);
 728                                                 divideInHalf(tempSeq, leftQuery, rightQuery);
 729
 730                                                 //run chimeraSlayer on each piece
 731                                                 Sequence* left = new Sequence(candidateSeq->getName(), leftQuery);
 732                                                 Sequence* right = new Sequence(candidateSeq->getName(), rightQuery);
 733
 734                                                 //find chimeras
 735                                                 chimera->getChimeras(left);
 736                                                 data_results leftResults = chimera->getResults();
 737
 738                                                 chimera->getChimeras(right);
 739                                                 data_results rightResults = chimera->getResults();
 740
 741                                                 //if either piece is chimeric then report
 742                                                 Sequence trimmed = chimera->print(out, out2, leftResults, rightResults);
 743                                                 if (trim) { trimmed.printSequence(out3);  }
 744
 745                                                 delete left; delete right;
 746
 747                                         }else { //already chimeric
 748                                                 //print results
 749                                                 Sequence trimmed = chimera->print(out, out2);
 750                                                 if (trim) { trimmed.printSequence(out3);  }
 751                                         }
 752
 753
 754                                 }
 755                                 count++;
 756                         }
 757
 758                         #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
 759                                 unsigned long int pos = inFASTA.tellg();
 760                                 if ((pos == -1) || (pos >= filePos->end)) { break; }
 761                         #else
 762                                 if (inFASTA.eof()) { break; }
 763                         #endif
 764
 765                         delete candidateSeq;
 766                         //report progress
 767                         if((count) % 100 == 0){ m->mothurOut("Processing sequence: " + toString(count)); m->mothurOutEndLine();         }
 768                 }
 769                 //report progress
 770                 if((count) % 100 != 0){ m->mothurOut("Processing sequence: " + toString(count)); m->mothurOutEndLine();         }
 771
 772                 out.close();
 773                 out2.close();
 774                 if (trim) { out3.close(); }
 775                 inFASTA.close();
 776
 777                 return count;
 778         }
 779         catch(exception& e) {
 780                 m->errorOut(e, "ChimeraSlayerCommand", "driver");
 781                 exit(1);
 782         }
 783 }
 784 //**********************************************************************************************************************
 785 #ifdef USE_MPI
 786 int ChimeraSlayerCommand::driverMPI(int start, int num, MPI_File& inMPI, MPI_File& outMPI, MPI_File& outAccMPI, MPI_File& outFastaMPI, vector<unsigned long int>& MPIPos){
 787         try {
 788                 MPI_Status status;
 789                 int pid;
 790                 MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
 791
 792                 for(int i=0;i<num;i++){
 793
 794                         if (m->control_pressed) {       return 1;       }
 795
 796                         //read next sequence
 797                         int length = MPIPos[start+i+1] - MPIPos[start+i];
 798
 799                         char* buf4 = new char[length];
 800                         MPI_File_read_at(inMPI, MPIPos[start+i], buf4, length, MPI_CHAR, &status);
 801
 802                         string tempBuf = buf4;
 803                         if (tempBuf.length() > length) { tempBuf = tempBuf.substr(0, length);  }
 804                         istringstream iss (tempBuf,istringstream::in);
 805
 806                         delete buf4;
 807
 808                         Sequence* candidateSeq = new Sequence(iss);  m->gobble(iss);
 809                         string candidateAligned = candidateSeq->getAligned();
 810
 811                         if (candidateSeq->getName() != "") { //incase there is a commented sequence at the end of a file
 812
 813                                 if (candidateSeq->getAligned().length() != templateSeqsLength) {
 814                                         m->mothurOut(candidateSeq->getName() + " is not the same length as the template sequences. Skipping."); m->mothurOutEndLine();
 815                                 }else{
 816
 817                                         //find chimeras
 818                                         chimera->getChimeras(candidateSeq);
 819
 820                                         if (m->control_pressed) {       delete candidateSeq; return 1;  }
 821
 822                                         //if you are not chimeric, then check each half
 823                                         data_results wholeResults = chimera->getResults();
 824
 825                                         //determine if we need to split
 826                                         bool isChimeric = false;
 827
 828                                         if (wholeResults.flag == "yes") {
 829                                                 string chimeraFlag = "no";
 830                                                 if(  (wholeResults.results[0].bsa >= minBS && wholeResults.results[0].divr_qla_qrb >= divR)
 831                                                    ||
 832                                                    (wholeResults.results[0].bsb >= minBS && wholeResults.results[0].divr_qlb_qra >= divR) ) { chimeraFlag = "yes"; }
 833
 834
 835                                                 if (chimeraFlag == "yes") {
 836                                                         if ((wholeResults.results[0].bsa >= minBS) || (wholeResults.results[0].bsb >= minBS)) { isChimeric = true; }
 837                                                 }
 838                                         }
 839
 840                                         if ((!isChimeric) && trimera) {
 841                                                 //split sequence in half by bases
 842                                                 string leftQuery, rightQuery;
 843                                                 Sequence tempSeq(candidateSeq->getName(), candidateAligned);
 844                                                 divideInHalf(tempSeq, leftQuery, rightQuery);
 845
 846                                                 //run chimeraSlayer on each piece
 847                                                 Sequence* left = new Sequence(candidateSeq->getName(), leftQuery);
 848                                                 Sequence* right = new Sequence(candidateSeq->getName(), rightQuery);
 849
 850                                                 //find chimeras
 851                                                 chimera->getChimeras(left);
 852                                                 data_results leftResults = chimera->getResults();
 853
 854                                                 chimera->getChimeras(right);
 855                                                 data_results rightResults = chimera->getResults();
 856
 857                                                 //if either piece is chimeric then report
 858                                                 Sequence trimmed = chimera->print(outMPI, outAccMPI, leftResults, rightResults);
 859                                                 if (trim) {
 860                                                         string outputString = ">" + trimmed.getName() + "\n" + trimmed.getAligned() + "\n";
 861
 862                                                         //write to accnos file
 863                                                         int length = outputString.length();
 864                                                         char* buf2 = new char[length];
 865                                                         memcpy(buf2, outputString.c_str(), length);
 866
 867                                                         MPI_File_write_shared(outFastaMPI, buf2, length, MPI_CHAR, &status);
 868                                                         delete buf2;
 869                                                 }
 870
 871                                                 delete left; delete right;
 872
 873                                         }else {
 874                                                 //print results
 875                                                 Sequence trimmed = chimera->print(outMPI, outAccMPI);
 876
 877                                                 if (trim) {
 878                                                         string outputString = ">" + trimmed.getName() + "\n" + trimmed.getAligned() + "\n";
 879
 880                                                         //write to accnos file
 881                                                         int length = outputString.length();
 882                                                         char* buf2 = new char[length];
 883                                                         memcpy(buf2, outputString.c_str(), length);
 884
 885                                                         MPI_File_write_shared(outFastaMPI, buf2, length, MPI_CHAR, &status);
 886                                                         delete buf2;
 887                                                 }
 888                                         }
 889
 890                                 }
 891                         }
 892                         delete candidateSeq;
 893
 894                         //report progress
 895                         if((i+1) % 100 == 0){  cout << "Processing sequence: " << (i+1) << endl;        m->mothurOutJustToLog("Processing sequence: " + toString(i+1) + "\n");          }
 896                 }
 897                 //report progress
 898                 if(num % 100 != 0){             cout << "Processing sequence: " << num << endl; m->mothurOutJustToLog("Processing sequence: " + toString(num) + "\n");  }
 899
 900
 901                 return 0;
 902         }
 903         catch(exception& e) {
 904                 m->errorOut(e, "ChimeraSlayerCommand", "driverMPI");
 905                 exit(1);
 906         }
 907 }
 908 #endif
 909
 910 /**************************************************************************************************/
 911
 912 int ChimeraSlayerCommand::createProcesses(string outputFileName, string filename, string accnos, string fasta) {
 913         try {
 914 #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
 915                 int process = 0;
 916                 int num = 0;
 917
 918                 //loop through and create all the processes you want
 919                 while (process != processors) {
 920                         int pid = fork();
 921
 922                         if (pid > 0) {
 923                                 processIDS.push_back(pid);  //create map from line number to pid so you can append files in correct order later
 924                                 process++;
 925                         }else if (pid == 0){
 926                                 num = driver(lines[process], outputFileName + toString(getpid()) + ".temp", filename, accnos + toString(getpid()) + ".temp", fasta + toString(getpid()) + ".temp");
 927
 928                                 //pass numSeqs to parent
 929                                 ofstream out;
 930                                 string tempFile = outputFileName + toString(getpid()) + ".num.temp";
 931                                 m->openOutputFile(tempFile, out);
 932                                 out << num << '\t' << chimera->getNumNoParents() << endl;
 933                                 out.close();
 934                                 exit(0);
 935                         }else {
 936                                 m->mothurOut("[ERROR]: unable to spawn the necessary processes."); m->mothurOutEndLine();
 937                                 for (int i = 0; i < processIDS.size(); i++) { kill (processIDS[i], SIGINT); }
 938                                 exit(0);
 939                         }
 940                 }
 941
 942                 //force parent to wait until all the processes are done
 943                 for (int i=0;i<processors;i++) {
 944                         int temp = processIDS[i];
 945                         wait(&temp);
 946                 }
 947
 948                 int numNoParents = 0;
 949                 for (int i = 0; i < processIDS.size(); i++) {
 950                         ifstream in;
 951                         string tempFile =  outputFileName + toString(processIDS[i]) + ".num.temp";
 952                         m->openInputFile(tempFile, in);
 953                         if (!in.eof()) { int tempNum = 0; int tempNumParents = 0; in >> tempNum >> tempNumParents; num += tempNum; numNoParents += tempNumParents; }
 954                         in.close(); remove(tempFile.c_str());
 955                 }
 956
 957                 if (num == numNoParents) {  m->mothurOut("[WARNING]: megablast returned 0 potential parents for all your sequences. This could be due to formatdb.exe not being setup properly, please check formatdb.log for errors."); m->mothurOutEndLine(); }
 958
 959                 return num;
 960 #endif
 961         }
 962         catch(exception& e) {
 963                 m->errorOut(e, "ChimeraSlayerCommand", "createProcesses");
 964                 exit(1);
 965         }
 966 }
 967
 968 /**************************************************************************************************/
 969
 970 int ChimeraSlayerCommand::divideInHalf(Sequence querySeq, string& leftQuery, string& rightQuery) {
 971         try {
 972
 973                 string queryUnAligned = querySeq.getUnaligned();
 974                 int numBases = int(queryUnAligned.length() * 0.5);
 975
 976                 string queryAligned = querySeq.getAligned();
 977                 leftQuery = querySeq.getAligned();
 978                 rightQuery = querySeq.getAligned();
 979
 980                 int baseCount = 0;
 981                 int leftSpot = 0;
 982                 for (int i = 0; i < queryAligned.length(); i++) {
 983                         //if you are a base
 984                         if (isalpha(queryAligned[i])) {
 985                                 baseCount++;
 986                         }
 987
 988                         //if you have half
 989                         if (baseCount >= numBases) {  leftSpot = i; break; } //first half
 990                 }
 991
 992                 //blank out right side
 993                 for (int i = leftSpot; i < leftQuery.length(); i++) { leftQuery[i] = '.'; }
 994
 995                 //blank out left side
 996                 for (int i = 0; i < leftSpot; i++) { rightQuery[i] = '.'; }
 997
 998                 return 0;
 999
1000         }
1001         catch(exception& e) {
1002                 m->errorOut(e, "ChimeraSlayerCommand", "divideInHalf");
1003                 exit(1);
1004         }
1005 }
1006 /**************************************************************************************************/
1007 map<string, int> ChimeraSlayerCommand::sortFastaFile(string fastaFile, string nameFile) {
1008         try {
1009                 map<string, int> nameAbund;
1010
1011                 //read through fastafile and store info
1012                 map<string, string> seqs;
1013                 ifstream in;
1014                 m->openInputFile(fastaFile, in);
1015
1016                 while (!in.eof()) {
1017
1018                         if (m->control_pressed) { in.close(); return nameAbund; }
1019
1020                         Sequence seq(in); m->gobble(in);
1021                         seqs[seq.getName()] = seq.getAligned();
1022                 }
1023
1024                 in.close();
1025
1026                 //read namefile
1027                 vector<seqPriorityNode> nameMapCount;
1028                 int error = m->readNames(nameFile, nameMapCount, seqs);
1029
1030                 if (m->control_pressed) { return nameAbund; }
1031
1032                 if (error == 1) { m->control_pressed = true; return nameAbund; }
1033                 if (seqs.size() != nameMapCount.size()) { m->mothurOut( "The number of sequences in your fastafile does not match the number of sequences in your namefile, aborting."); m->mothurOutEndLine(); m->control_pressed = true; return nameAbund; }
1034
1035                 sort(nameMapCount.begin(), nameMapCount.end(), compareSeqPriorityNodes);
1036
1037                 string newFasta = fastaFile + ".temp";
1038                 ofstream out;
1039                 m->openOutputFile(newFasta, out);
1040
1041                 //print new file in order of
1042                 for (int i = 0; i < nameMapCount.size(); i++) {
1043                         out << ">" << nameMapCount[i].name << endl << nameMapCount[i].seq << endl;
1044                         nameAbund[nameMapCount[i].name] = nameMapCount[i].numIdentical;
1045                 }
1046                 out.close();
1047
1048                 rename(newFasta.c_str(), fastaFile.c_str());
1049
1050                 return nameAbund;
1051
1052         }
1053         catch(exception& e) {
1054                 m->errorOut(e, "ChimeraSlayerCommand", "sortFastaFile");
1055                 exit(1);
1056         }
1057 }
1058
1059 /**************************************************************************************************/
1060