chimeraseqscommand.cpp

   1 /*
   2  *  chimeraseqscommand.cpp
   3  *  Mothur
   4  *
   5  *  Created by Sarah Westcott on 6/29/09.
   6  *  Copyright 2009 Schloss Lab UMASS Amherst. All rights reserved.
   7  *
   8  */
   9
  10 #include "chimeraseqscommand.h"
  11 #include "bellerophon.h"
  12 #include "pintail.h"
  13 #include "ccode.h"
  14 #include "chimeracheckrdp.h"
  15 #include "chimeraslayer.h"
  16
  17
  18 //***************************************************************************************************************
  19
  20 ChimeraSeqsCommand::ChimeraSeqsCommand(string option){
  21         try {
  22                 abort = false;
  23
  24                 //allow user to run help
  25                 if(option == "help") { help(); abort = true; }
  26
  27                 else {
  28                         //valid paramters for this command
  29                         string Array[] =  {"fasta", "filter", "correction", "processors", "method", "window", "increment", "template", "conservation", "quantile", "mask",
  30                         "numwanted", "ksize", "svg", "name", "match","mismatch", "divergence", "minsim","mincov","minbs", "minsnp","parents", "iters","outputdir","inputdir", "search","realign" };
  31                         vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
  32
  33                         OptionParser parser(option);
  34                         map<string,string> parameters = parser.getParameters();
  35
  36                         ValidParameters validParameter;
  37                         map<string,string>::iterator it;
  38
  39                         //check to make sure all parameters are valid for command
  40                         for (it = parameters.begin(); it != parameters.end(); it++) {
  41                                 if (validParameter.isValidParameter(it->first, myArray, it->second) != true) {  abort = true;  }
  42                         }
  43
  44                         //if the user changes the input directory command factory will send this info to us in the output parameter
  45                         string inputDir = validParameter.validFile(parameters, "inputdir", false);
  46                         if (inputDir == "not found"){   inputDir = "";          }
  47                         else {
  48                                 string path;
  49                                 it = parameters.find("fasta");
  50                                 //user has given a template file
  51                                 if(it != parameters.end()){
  52                                         path = hasPath(it->second);
  53                                         //if the user has not given a path then, add inputdir. else leave path alone.
  54                                         if (path == "") {       parameters["fasta"] = inputDir + it->second;            }
  55                                 }
  56
  57                                 it = parameters.find("template");
  58                                 //user has given a template file
  59                                 if(it != parameters.end()){
  60                                         path = hasPath(it->second);
  61                                         //if the user has not given a path then, add inputdir. else leave path alone.
  62                                         if (path == "") {       parameters["template"] = inputDir + it->second;         }
  63                                 }
  64
  65                                 it = parameters.find("conservation");
  66                                 //user has given a template file
  67                                 if(it != parameters.end()){
  68                                         path = hasPath(it->second);
  69                                         //if the user has not given a path then, add inputdir. else leave path alone.
  70                                         if (path == "") {       parameters["conservation"] = inputDir + it->second;             }
  71                                 }
  72
  73                                 it = parameters.find("quantile");
  74                                 //user has given a template file
  75                                 if(it != parameters.end()){
  76                                         path = hasPath(it->second);
  77                                         //if the user has not given a path then, add inputdir. else leave path alone.
  78                                         if (path == "") {       parameters["quantile"] = inputDir + it->second;         }
  79                                 }
  80
  81                                 it = parameters.find("name");
  82                                 //user has given a template file
  83                                 if(it != parameters.end()){
  84                                         path = hasPath(it->second);
  85                                         //if the user has not given a path then, add inputdir. else leave path alone.
  86                                         if (path == "") {       parameters["name"] = inputDir + it->second;             }
  87                                 }
  88                         }
  89
  90
  91                         //check for required parameters
  92                         fastafile = validParameter.validFile(parameters, "fasta", true);
  93                         if (fastafile == "not open") { abort = true; }
  94                         else if (fastafile == "not found") { fastafile = ""; mothurOut("fasta is a required parameter for the chimera.seqs command."); mothurOutEndLine(); abort = true;  }
  95
  96                         //if the user changes the output directory command factory will send this info to us in the output parameter
  97                         outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){
  98                                 outputDir = "";
  99                                 outputDir += hasPath(fastafile); //if user entered a file with a path then preserve it
 100                         }
 101
 102                         templatefile = validParameter.validFile(parameters, "template", true);
 103                         if (templatefile == "not open") { abort = true; }
 104                         else if (templatefile == "not found") { templatefile = "";  }
 105
 106                         consfile = validParameter.validFile(parameters, "conservation", true);
 107                         if (consfile == "not open") { abort = true; }
 108                         else if (consfile == "not found") { consfile = "";  }
 109
 110                         quanfile = validParameter.validFile(parameters, "quantile", true);
 111                         if (quanfile == "not open") { abort = true; }
 112                         else if (quanfile == "not found") { quanfile = "";  }
 113
 114                         namefile = validParameter.validFile(parameters, "name", true);
 115                         if (namefile == "not open") { abort = true; }
 116                         else if (namefile == "not found") { namefile = "";  }
 117
 118                         maskfile = validParameter.validFile(parameters, "mask", false);
 119                         if (maskfile == "not found") { maskfile = "";  }
 120                         else if (maskfile != "default")  {
 121                                 if (inputDir != "") {
 122                                         string path = hasPath(maskfile);
 123                                         //if the user has not given a path then, add inputdir. else leave path alone.
 124                                         if (path == "") {       maskfile = inputDir + maskfile;         }
 125                                 }
 126
 127                                 ifstream in;
 128                                 int     ableToOpen = openInputFile(maskfile, in);
 129                                 if (ableToOpen == 1) { abort = true; }
 130                                 in.close();
 131                         }
 132
 133                         method = validParameter.validFile(parameters, "method", false);                 if (method == "not found") { method = "pintail"; }
 134
 135                         string temp;
 136                         temp = validParameter.validFile(parameters, "filter", false);                   if (temp == "not found") { temp = "F"; }
 137                         filter = isTrue(temp);
 138
 139                         temp = validParameter.validFile(parameters, "correction", false);               if (temp == "not found") { temp = "T"; }
 140                         correction = isTrue(temp);
 141
 142                         temp = validParameter.validFile(parameters, "processors", false);               if (temp == "not found") { temp = "1"; }
 143                         convert(temp, processors);
 144
 145                         temp = validParameter.validFile(parameters, "ksize", false);                    if (temp == "not found") { temp = "7"; }
 146                         convert(temp, ksize);
 147
 148                         temp = validParameter.validFile(parameters, "svg", false);                              if (temp == "not found") { temp = "F"; }
 149                         svg = isTrue(temp);
 150
 151                         temp = validParameter.validFile(parameters, "window", false);
 152                         if ((temp == "not found") && (method == "chimeraslayer")) { temp = "50"; }
 153                         else if (temp == "not found") { temp = "0"; }
 154                         convert(temp, window);
 155
 156                         temp = validParameter.validFile(parameters, "match", false);                    if (temp == "not found") { temp = "5"; }
 157                         convert(temp, match);
 158
 159                         temp = validParameter.validFile(parameters, "mismatch", false);                 if (temp == "not found") { temp = "-4"; }
 160                         convert(temp, mismatch);
 161
 162                         temp = validParameter.validFile(parameters, "divergence", false);               if (temp == "not found") { temp = "1.007"; }
 163                         convert(temp, divR);
 164
 165                         temp = validParameter.validFile(parameters, "minsim", false);                   if (temp == "not found") { temp = "90"; }
 166                         convert(temp, minSimilarity);
 167
 168                         temp = validParameter.validFile(parameters, "mincov", false);                   if (temp == "not found") { temp = "70"; }
 169                         convert(temp, minCoverage);
 170
 171                         temp = validParameter.validFile(parameters, "minbs", false);                    if (temp == "not found") { temp = "90"; }
 172                         convert(temp, minBS);
 173
 174                         temp = validParameter.validFile(parameters, "minsnp", false);                   if (temp == "not found") { temp = "10"; }
 175                         convert(temp, minSNP);
 176
 177                         temp = validParameter.validFile(parameters, "parents", false);                  if (temp == "not found") { temp = "3"; }
 178                         convert(temp, parents);
 179
 180                         temp = validParameter.validFile(parameters, "realign", false);                  if (temp == "not found") { temp = "f"; }
 181                         realign = isTrue(temp);
 182
 183                         search = validParameter.validFile(parameters, "search", false);                 if (search == "not found") { search = "distance"; }
 184
 185                         temp = validParameter.validFile(parameters, "iters", false);
 186                         if ((temp == "not found") && (method == "chimeraslayer")) { temp = "100"; }
 187                         else if (temp == "not found") { temp = "1000"; }
 188                         convert(temp, iters);
 189
 190                         temp = validParameter.validFile(parameters, "increment", false);
 191                         if ((temp == "not found") && (method == "chimeracheck")) { temp = "10"; }
 192                         else if ((temp == "not found") && (method == "chimeraslayer")) { temp = "5"; }
 193                         else if (temp == "not found") { temp = "25"; }
 194                         convert(temp, increment);
 195
 196                         temp = validParameter.validFile(parameters, "numwanted", false);
 197                         if ((temp == "not found") && (method == "chimeraslayer")) { temp = "15"; }
 198                         else if (temp == "not found") { temp = "20"; }
 199                         convert(temp, numwanted);
 200
 201                         if ((search != "distance") && (search != "blast") && (search != "kmer")) { mothurOut(search + " is not a valid search."); mothurOutEndLine(); abort = true;  }
 202
 203                         if (((method != "bellerophon")) && (templatefile == "")) { mothurOut("You must provide a template file with the pintail, ccode, chimeraslayer or chimeracheck methods."); mothurOutEndLine(); abort = true;  }
 204
 205
 206                 }
 207         }
 208         catch(exception& e) {
 209                 errorOut(e, "ChimeraSeqsCommand", "ChimeraSeqsCommand");
 210                 exit(1);
 211         }
 212 }
 213 //**********************************************************************************************************************
 214
 215 void ChimeraSeqsCommand::help(){
 216         try {
 217
 218                 //"fasta", "filter", "correction", "processors", "method", "window", "increment", "template", "conservation", "quantile", "mask", "numwanted", "ksize", "svg", "name"
 219                 //mothurOut("chimera.seqs ASSUMES that your sequences are ALIGNED and if using a template that the template file sequences are the same length as the fasta file sequences.\n\n");
 220                 mothurOut("The chimera.seqs command reads a fastafile and creates list of potentially chimeric sequences.\n");
 221                 mothurOut("The chimera.seqs command parameters are fasta, filter, correction, processors, mask, method, window, increment, template, conservation, quantile, numwanted, ksize, svg, name, iters, search, realign.\n");
 222                 mothurOut("The fasta parameter is always required and template is required if using pintail, ccode or chimeracheck.\n");
 223                 mothurOut("The filter parameter allows you to specify if you would like to apply a vertical and 50% soft filter. \n");
 224                 mothurOut("The correction parameter allows you to put more emphasis on the distance between highly similar sequences and less emphasis on the differences between remote homologs.\n");
 225                 mothurOut("The processors parameter allows you to specify how many processors you would like to use.  The default is 1. \n");
 226                 mothurOut("The method parameter allows you to specify the method for finding chimeric sequences.  The default is pintail. Options include bellerophon, ccode and chimeracheck \n");
 227                 mothurOut("The mask parameter allows you to specify a file containing one sequence you wish to use as a mask for the your sequences. \n");
 228                 mothurOut("The window parameter allows you to specify the window size for searching for chimeras. \n");
 229                 mothurOut("The increment parameter allows you to specify how far you move each window while finding chimeric sequences.\n");
 230                 mothurOut("The template parameter allows you to enter a template file containing known non-chimeric sequences. \n");
 231                 mothurOut("The conservation parameter allows you to enter a frequency file containing the highest bases frequency at each place in the alignment.\n");
 232                 mothurOut("The quantile parameter allows you to enter a file containing quantiles for a template files sequences.\n");
 233                 mothurOut("The numwanted parameter allows you to specify how many sequences you would each query sequence compared with.\n");
 234                 mothurOut("The ksize parameter allows you to input kmersize. \n");
 235                 mothurOut("The svg parameter allows you to specify whether or not you would like a svg file outputted for each query sequence.\n");
 236                 mothurOut("The name parameter allows you to enter a file containing names of sequences you would like .svg files for.\n");
 237                 mothurOut("The iters parameter allows you to specify the number of bootstrap iters to do with the chimeraslayer method.\n");
 238                 mothurOut("The minsim parameter allows you .... \n");
 239                 mothurOut("The mincov parameter allows you to specify minimum coverage by closest matches found in template. Default is 70, meaning 70%. \n");
 240                 mothurOut("The minbs parameter allows you to specify minimum bootstrap support for calling a sequence chimeric. Default is 90, meaning 90%. \n");
 241                 mothurOut("The minsnp parameter allows you to specify percent of SNPs to sample on each side of breakpoint for computing bootstrap support (default: 10) \n");
 242                 mothurOut("The search parameter allows you to specify search method for finding the closest parent. Choices are distance, blast, and kmer, default distance.  -used only by chimeraslayer. \n");
 243                 mothurOut("The realign parameter allows you to realign the query to the potential paretns. Choices are true or false, default false.  -used only by chimeraslayer. \n");
 244                 mothurOut("NOT ALL PARAMETERS ARE USED BY ALL METHODS. Please look below for method specifics.\n\n");
 245                 mothurOut("Details for each method: \n");
 246                 mothurOut("\tpintail: \n");
 247                 mothurOut("\t\tparameters: fasta=required, template=required, filter=F, mask=no mask, processors=1, window=300, increment=25, conservation=not required, but will improve speed, quantile=not required, but will greatly improve speed. \n");
 248                 mothurOut("\t\tIf you have run chimera.seqs using pintail a .quan and .freq file will be created for your template, if you have not provided them for use in future command executions.\n");
 249                 mothurOut("\tbellerophon: \n");
 250                 mothurOut("\t\tparameters: fasta=required, filter=F, processors=1, window=1/4 length of seq, increment=25, correction=T. \n");
 251                 mothurOut("\tccode: \n");
 252                 mothurOut("\t\tparameters: fasta=required, template=required, filter=F, mask=no mask, processors=1, window=10% of length, numwanted=20\n");
 253                 mothurOut("\tchimeracheck: \n");
 254                 mothurOut("\t\tparameters: fasta=required, template=required, processors=1, increment=10, ksize=7, svg=F, name=none\n\n");
 255                 mothurOut("\tchimeraslayer: \n");
 256                 mothurOut("\t\tparameters: fasta=required, template=required, processors=1, increment=10, mask=no mask, numwanted=10, match=5, mismatch=-4, divergence=1.0, minsim=90, parents=5, iters=1000, window=100. \n\n");
 257                 mothurOut("The chimera.seqs command should be in the following format: \n");
 258                 mothurOut("chimera.seqs(fasta=yourFastaFile, filter=yourFilter, correction=yourCorrection, processors=yourProcessors, method=bellerophon) \n");
 259                 mothurOut("Example: chimera.seqs(fasta=AD.align, filter=True, correction=true, method=bellerophon, window=200) \n");
 260                 mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFastaFile).\n\n");
 261         }
 262         catch(exception& e) {
 263                 errorOut(e, "ChimeraSeqsCommand", "help");
 264                 exit(1);
 265         }
 266 }
 267
 268 //***************************************************************************************************************
 269
 270 ChimeraSeqsCommand::~ChimeraSeqsCommand(){      /*      do nothing      */      }
 271
 272 //***************************************************************************************************************
 273
 274 int ChimeraSeqsCommand::execute(){
 275         try{
 276
 277                 if (abort == true) { return 0; }
 278
 279                 int start = time(NULL);
 280
 281                 if (method == "bellerophon")                    {               chimera = new Bellerophon(fastafile, outputDir);                        }
 282                 else if (method == "pintail")                   {               chimera = new Pintail(fastafile, outputDir);                            }
 283                 else if (method == "ccode")                             {               chimera = new Ccode(fastafile, outputDir);                                      }
 284                 else if (method == "chimeracheck")              {               chimera = new ChimeraCheckRDP(fastafile, outputDir);            }
 285                 else if (method == "chimeraslayer")             {               chimera = new ChimeraSlayer(search, realign, fastafile);        }
 286                 else { mothurOut("Not a valid method."); mothurOutEndLine(); return 0;          }
 287
 288                 //set user options
 289                 if (maskfile == "default") { mothurOut("I am using the default 236627 EU009184.1 Shigella dysenteriae str. FBD013."); mothurOutEndLine();  }
 290
 291                 chimera->setCons(consfile);
 292                 chimera->setQuantiles(quanfile);
 293                 chimera->setMask(maskfile);
 294                 chimera->setFilter(filter);
 295                 chimera->setCorrection(correction);
 296                 chimera->setProcessors(processors);
 297                 chimera->setWindow(window);
 298                 chimera->setIncrement(increment);
 299                 chimera->setNumWanted(numwanted);
 300                 chimera->setKmerSize(ksize);
 301                 chimera->setSVG(svg);
 302                 chimera->setName(namefile);
 303                 chimera->setMatch(match);
 304                 chimera->setMisMatch(mismatch);
 305                 chimera->setDivR(divR);
 306                 chimera->setParents(parents);
 307                 chimera->setMinSim(minSimilarity);
 308                 chimera->setMinCoverage(minCoverage);
 309                 chimera->setMinBS(minBS);
 310                 chimera->setMinSNP(minSNP);
 311                 chimera->setIters(iters);
 312                 chimera->setTemplateFile(templatefile);
 313
 314
 315
 316                 vector<Sequence*> templateSeqs;
 317                 if ((method != "bellerophon") && (method != "chimeracheck")) {
 318                         templateSeqs = chimera->readSeqs(templatefile);
 319                         if (chimera->getUnaligned()) {
 320                                 mothurOut("Your sequences need to be aligned when you use the chimeraslayer method."); mothurOutEndLine();
 321                                 //free memory
 322                                 for (int i = 0; i < templateSeqs.size(); i++)           {  delete templateSeqs[i];              }
 323                                 return 0;
 324                         }
 325
 326                         //set options
 327                         chimera->setTemplateSeqs(templateSeqs);
 328
 329                 }else if (method == "bellerophon") {//run bellerophon separately since you need to read entire fastafile to run it
 330                         chimera->getChimeras();
 331
 332                         string outputFName = outputDir + getRootName(getSimpleName(fastafile)) + method + maskfile + ".chimeras";
 333                         ofstream out;
 334                         openOutputFile(outputFName, out);
 335
 336                         chimera->print(out);
 337                         out.close();
 338                         return 0;
 339                 }
 340
 341                 //some methods need to do prep work before processing the chimeras
 342                 chimera->doPrep();
 343
 344                 ofstream outHeader;
 345                 string tempHeader = outputDir + getRootName(getSimpleName(fastafile)) + method + maskfile + ".chimeras.tempHeader";
 346                 openOutputFile(tempHeader, outHeader);
 347
 348                 chimera->printHeader(outHeader);
 349                 outHeader.close();
 350
 351                 string outputFileName = outputDir + getRootName(getSimpleName(fastafile)) + method + maskfile + ".chimeras";
 352
 353                 //break up file
 354                 #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
 355                         if(processors == 1){
 356                                 ifstream inFASTA;
 357                                 openInputFile(fastafile, inFASTA);
 358                                 numSeqs=count(istreambuf_iterator<char>(inFASTA),istreambuf_iterator<char>(), '>');
 359                                 inFASTA.close();
 360
 361                                 lines.push_back(new linePair(0, numSeqs));
 362
 363                                 driver(lines[0], outputFileName, fastafile);
 364
 365                         }else{
 366                                 vector<int> positions;
 367                                 processIDS.resize(0);
 368
 369                                 ifstream inFASTA;
 370                                 openInputFile(fastafile, inFASTA);
 371
 372                                 string input;
 373                                 while(!inFASTA.eof()){
 374                                         input = getline(inFASTA);
 375                                         if (input.length() != 0) {
 376                                                 if(input[0] == '>'){    long int pos = inFASTA.tellg(); positions.push_back(pos - input.length() - 1);  }
 377                                         }
 378                                 }
 379                                 inFASTA.close();
 380
 381                                 numSeqs = positions.size();
 382
 383                                 int numSeqsPerProcessor = numSeqs / processors;
 384
 385                                 for (int i = 0; i < processors; i++) {
 386                                         long int startPos = positions[ i * numSeqsPerProcessor ];
 387                                         if(i == processors - 1){
 388                                                 numSeqsPerProcessor = numSeqs - i * numSeqsPerProcessor;
 389                                         }
 390                                         lines.push_back(new linePair(startPos, numSeqsPerProcessor));
 391                                 }
 392
 393
 394                                 createProcesses(outputFileName, fastafile);
 395
 396                                 rename((outputFileName + toString(processIDS[0]) + ".temp").c_str(), outputFileName.c_str());
 397
 398                                 //append alignment and report files
 399                                 for(int i=1;i<processors;i++){
 400                                         appendOutputFiles((outputFileName + toString(processIDS[i]) + ".temp"), outputFileName);
 401                                         remove((outputFileName + toString(processIDS[i]) + ".temp").c_str());
 402                                 }
 403                         }
 404
 405                 #else
 406                         ifstream inFASTA;
 407                         openInputFile(candidateFileNames[s], inFASTA);
 408                         numSeqs=count(istreambuf_iterator<char>(inFASTA),istreambuf_iterator<char>(), '>');
 409                         inFASTA.close();
 410                         lines.push_back(new linePair(0, numSeqs));
 411
 412                         driver(lines[0], outputFileName, fastafile);
 413                 #endif
 414
 415                 //mothurOut("Output File Names: ");
 416                 //if ((filter) && (method == "bellerophon")) { mothurOut(
 417                 //if (outputDir == "") { fastafile = getRootName(fastafile) + "filter.fasta"; }
 418                 //      else                             { fastafile = outputDir + getRootName(getSimpleName(fastafile)) + "filter.fasta"; }
 419
 420                 appendOutputFiles(tempHeader, outputFileName);
 421                 remove(outputFileName.c_str());
 422                 rename(tempHeader.c_str(), outputFileName.c_str());
 423
 424                 for (int i = 0; i < templateSeqs.size(); i++)           {   delete templateSeqs[i];     }
 425
 426                 if (method == "chimeracheck") { mothurOutEndLine(); mothurOut("This method does not determine if a sequence is chimeric, but allows you to make that determination based on the IS values."); mothurOutEndLine();  }
 427
 428                 mothurOutEndLine(); mothurOut("It took " + toString(time(NULL) - start) + " secs to check " + toString(numSeqs) + " sequences.");       mothurOutEndLine();
 429
 430                 return 0;
 431
 432         }
 433         catch(exception& e) {
 434                 errorOut(e, "ChimeraSeqsCommand", "execute");
 435                 exit(1);
 436         }
 437 }//**********************************************************************************************************************
 438
 439 int ChimeraSeqsCommand::driver(linePair* line, string outputFName, string filename){
 440         try {
 441                 ofstream out;
 442                 openOutputFile(outputFName, out);
 443
 444                 ifstream inFASTA;
 445                 openInputFile(filename, inFASTA);
 446
 447                 inFASTA.seekg(line->start);
 448
 449                 for(int i=0;i<line->numSeqs;i++){
 450
 451                         Sequence* candidateSeq = new Sequence(inFASTA);  gobble(inFASTA);
 452
 453                         if (candidateSeq->getName() != "") { //incase there is a commented sequence at the end of a file
 454
 455                                 //find chimeras
 456                                 chimera->getChimeras(candidateSeq);
 457
 458                                 //print results
 459                                 chimera->print(out);
 460                         }
 461                         delete candidateSeq;
 462
 463                         //report progress
 464                         if((i+1) % 100 == 0){   mothurOut("Processing sequence: " + toString(i+1)); mothurOutEndLine();         }
 465                 }
 466                 //report progress
 467                 if((line->numSeqs) % 100 != 0){ mothurOut("Processing sequence: " + toString(line->numSeqs)); mothurOutEndLine();               }
 468
 469                 out.close();
 470                 inFASTA.close();
 471
 472                 return 1;
 473         }
 474         catch(exception& e) {
 475                 errorOut(e, "ChimeraSeqsCommand", "driver");
 476                 exit(1);
 477         }
 478 }
 479
 480 /**************************************************************************************************/
 481
 482 void ChimeraSeqsCommand::createProcesses(string outputFileName, string filename) {
 483         try {
 484 #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
 485                 int process = 0;
 486                 //              processIDS.resize(0);
 487
 488                 //loop through and create all the processes you want
 489                 while (process != processors) {
 490                         int pid = fork();
 491
 492                         if (pid > 0) {
 493                                 processIDS.push_back(pid);  //create map from line number to pid so you can append files in correct order later
 494                                 process++;
 495                         }else if (pid == 0){
 496                                 driver(lines[process], outputFileName + toString(getpid()) + ".temp", filename);
 497                                 exit(0);
 498                         }else { mothurOut("unable to spawn the necessary processes."); mothurOutEndLine(); exit(0); }
 499                 }
 500
 501                 //force parent to wait until all the processes are done
 502                 for (int i=0;i<processors;i++) {
 503                         int temp = processIDS[i];
 504                         wait(&temp);
 505                 }
 506 #endif
 507         }
 508         catch(exception& e) {
 509                 errorOut(e, "ChimeraSeqsCommand", "createProcesses");
 510                 exit(1);
 511         }
 512 }
 513
 514 /**************************************************************************************************/
 515
 516 void ChimeraSeqsCommand::appendOutputFiles(string temp, string filename) {
 517         try{
 518
 519                 ofstream output;
 520                 ifstream input;
 521
 522                 openOutputFileAppend(temp, output);
 523                 openInputFile(filename, input);
 524
 525                 while(char c = input.get()){
 526                         if(input.eof())         {       break;                  }
 527                         else                            {       output << c;    }
 528                 }
 529
 530                 input.close();
 531                 output.close();
 532         }
 533         catch(exception& e) {
 534                 errorOut(e, "ChimeraSeqsCommand", "appendOuputFiles");
 535                 exit(1);
 536         }
 537 }
 538 //**********************************************************************************************************************
 539
 540