$(MODULE) load STAR/2.4.2a; \
mkdir -p $(SRX)_star; \
STAR --outFileNamePrefix $(SRX)_star/ \
- --outSAMtype BAM SortedByCoordinate \
- --runThreadN $(CORES) \
- --outSAMstrandField intronMotif \
- --genomeDir $(STAR_INDEX_DIR) \
- --readFilesCommand "gzip -dc" \
- --readFilesIn $(TOPHAT_FASTQ_ARGUMENT);
- ln $(SRX)_star/Aligned.sortedByCoord.out.bam $@ -s
+ --outSAMtype BAM SortedByCoordinate \
+ --runThreadN $(CORES) \
+ --outSAMstrandField intronMotif \
+ --genomeDir $(STAR_INDEX_DIR) \
+ --readFilesCommand "gzip -dc" \
+ --readFilesIn $(TOPHAT_FASTQ_ARGUMENT);
+ ln $(SRX)_star/Aligned.*.bam $@ -s
\end{minted} %$
\begin{itemize}
\item Call STAR with lots of options to do the alignment
\end{frame}
\begin{frame}[fragile]{Run it on biocluster}
- \begin{minted}[shell]
-for a in SRX*/Makefile; do
- (cd $(dirname $a);
- MAKE_TARGET=call qsub -q default -v MAKE_TARGET -S /bin/bash \
- -d "$(pwd)" -l "nodes=1:ppn=8,mem=12G" \
- ~donarm/uiuc_igb_scripts/run_make
- );
-done;
-\end{minted} %$
+ \begin{minted}{shell}
+~donarm/uiuc_igb_scripts/dqsub --mem 70G \
+ --ppn 8 make call;
+\end{minted}
+\begin{itemize}
+\item dqsub is my own qsub wrapper which avoids me having to write
+ little scripts for everything
+\item \url{http://git.donarmstrong.com/?p=uiuc_igb_scripts.git;a=blob;f=dqsub}
+\end{itemize}
\end{frame}
\section{Why not make?}
+\begin{frame}{Why not make?}
+ \begin{itemize}
+ \item Timestamps, not MD5sums
+ \item Complicated workflows
+ \end{itemize}
+\end{frame}
+
\subsection{Timestamps}
+\begin{frame}{Dealing with timestamps}
+ \begin{itemize}
+ \item Make builds things on the basis of timestamps
+ \item But what if the contents haven't changed and it's expensive to
+ rebuild?
+ \item Use md5sum!
+ \end{itemize}
+\begin{minted}[showtabs,breaklines]{make}
+TARGET: PREREQ1 PREREQ1
+ if [ -e $@.target.md5sum ] && [ -e $@ ] && \
+ md5sum --status --check $@.target.md5sum; \
+ then \
+ touch $@; \
+ else \
+ RECIPE FOR $@; \
+ md5sum $^ > $@.target.md5sum; \
+ fi;
+\end{minted}
+\end{frame}
+
\subsection{Complicated Workflows}
+\begin{frame}{Complicated Workflow}
+
+\end{frame}
+
\section{Further Resources}
+\begin{frame}{Further Resources}
+\end{frame}
\end{document}