### II. Calculating Expression Values
-To prepare the reference sequences, you should run the
+To calculate expression values, you should run the
'rsem-calculate-expression' program. Run
rsem-calculate-expression --help
to get usage information or visit the [rsem-calculate-expression
documentation page](rsem-calculate-expression.html).
-Note: RSEM no longer provides nu values. Instead, RSEM provides
-nrf(normalized read fraction), which is a normalized version of theta
-vector excluding theta_0.
-
#### Calculating expression values from single-end data
For single-end models, users have the option of providing a fragment