### Prerequisites
+C++ and Perl are required to be installed.
+
To take advantage of RSEM's built-in support for the Bowtie alignment
program, you must have [Bowtie](http://bowtie-bio.sourceforge.net) installed.
+If you want to plot model learned by RSEM, you should also install R.
+
## <a name="usage"></a> Usage
### I. Preparing Reference Sequences