+++ /dev/null
-IsoSimuAt<-function(DVDconstant=NULL, DVDqt1=NULL, DVDqt2=NULL, Conditions, NumofSample, NumofIso=NULL, DEIsoProp, Phiconstant=NULL, Phi.qt1=NULL, Phi.qt2=NULL,NormFactor=NULL, OnlyData=T)
-{
-#Ng paired 2012 feb 1
-if(is.null(NormFactor)) NormFactor=rep(1,NumofSample)
-data(IsoEBresultGouldBart2)
-
-MeansC1=IsoEBresultGouldBart2$C1Mean
-MeansC2=IsoEBresultGouldBart2$C2Mean
-MeanDVD=sapply(1:9,function(i) MeansC1[[i]]/MeansC2[[i]])
-if (length(DVDconstant)==0) DVDLibrary= unlist(MeanDVD)[unlist(MeanDVD)<quantile(unlist(MeanDVD)[unlist(MeanDVD)!=Inf],DVDqt2) & unlist(MeanDVD)>quantile(unlist(MeanDVD)[unlist(MeanDVD)!=Inf],DVDqt1)]
-
-
-
-
-VarInput=IsoEBresultGouldBart2$VarList
-VarInputNg=list(VarInput[[1]],unlist(VarInput[c(2,4,6,8)]),unlist(VarInput[c(3,5,7,9)]))
-
-if(length(NumofIso)==0) NumofIso=sapply(1:3,function(i)length(VarInputNg[[i]]))
-PhiInput.raw=IsoEBresultGouldBart2$RList
-PhiInput.raw.Ng=list(PhiInput.raw[[1]],unlist(PhiInput.raw[c(2,4,6,8)]),unlist(PhiInput.raw[c(3,5,7,9)]))
-
-
-if (length(Phiconstant)==0){
- PhiLibrary=sapply(1:3,function(i)PhiInput.raw.Ng[[i]][1/PhiInput.raw.Ng[[i]]<quantile(1/PhiInput.raw.Ng[[i]],Phi.qt2) & 1/PhiInput.raw.Ng[[i]]>quantile(1/PhiInput.raw.Ng[[i]],Phi.qt1)],simplify=F)
- PhiIndex=sapply(1:3, function(i)sample(names(PhiLibrary[[i]]),NumofIso[[i]],replace=T),simplify=F)
- PhiInputNg=sapply(1:3, function(i)PhiLibrary[[i]][PhiIndex[[i]]])
-}
-if (length(Phiconstant)!=0)PhiInputNg=sapply(1:3,function(i)rep(Phiconstant,NumofIso[[i]]),simplify=F)
-
-# Wanna DENumbers be proportion to 2
-DEIsoNumbers=round(NumofIso*DEIsoProp/2)*2
-IsoNames=sapply(1:3,function(i)paste("I",i,c(1:NumofIso[i]),sep="_"),simplify=F)
-MeanNg=list(IsoEBresultGouldBart2$MeanList[[1]],unlist(IsoEBresultGouldBart2$MeanList[c(2,4,6,8)]),
-unlist(IsoEBresultGouldBart2$MeanList[c(3,5,7,9)]))
-MeanInputNg=sapply(1:3, function(i)MeanNg[[i]][PhiIndex[[i]]])
-
-for(i in 1:3){
- names(MeanInputNg[[i]])=IsoNames[[i]]
- names(PhiInputNg[[i]])=IsoNames[[i]]
- }
-
-#########
-# data
-#########
-EEList=sapply(1:3,function(i) sapply(1:NumofIso[[i]], function(j)sapply(1:NumofSample,function(h) rnbinom(1,mu=MeanInputNg[[i]][j]*NormFactor[h], size=PhiInputNg[[i]][j]))),simplify=F)
-
-
-generateDataraw=vector("list",3)
-MeanVector=vector("list",3)
-VarVector=vector("list",3)
-MOV.post=vector("list",3)
-
-
-for(g in 1:3){
- generateDataraw[[g]]=t(EEList[[g]][,1:NumofIso[g]])
- if(length(DVDconstant)==0){
- for(j in 1:NumofIso[g]){
- if (j<=(DEIsoNumbers[g]/2)) generateDataraw[[g]][j,((NumofSample/2)+1):NumofSample]=sapply((NumofSample/2+1):NumofSample, function(h)rnbinom(1, size=PhiInputNg[[g]][j], mu=sample(DVDLibrary,1)*MeanInputNg[[g]][j]*NormFactor[h]), simplify=T)
- if (j>=((DEIsoNumbers[g]/2)+1) & j <=DEIsoNumbers[g]) generateDataraw[[g]][j,1:(NumofSample/2)]=sapply(1:(NumofSample/2),function(h) rnbinom(1, size=MeanInputNg[[g]][j], mu= sample(DVDLibrary,1)*MeanInputNg[[g]][j]*NormFactor[h]),simplify=T)
-}
- }
- if(length(DVDconstant)!=0){
- for(j in 1:NumofIso[g]){
- if (j<=(DEIsoNumbers[g]/2)) generateDataraw[[g]][j,((NumofSample/2)+1):NumofSample]=sapply((NumofSample/2+1):NumofSample, function(h)rnbinom(1, DVDconstant*MeanInputNg[[g]][j]*NormFactor[h]),simplify=T)
- if (j>=((DEIsoNumbers[g]/2)+1) & j <=DEIsoNumbers[g]) generateDataraw[[g]][j,1:(NumofSample/2)]=sapply(1:(NumofSample/2),function(h) rnbinom(1, DVDconstant*MeanInputNg[[g]][j]*NormFactor[h]),simplify=T)
- }
- }
-rownames(generateDataraw[[g]])=IsoNames[[g]][1:NumofIso[g]]
-MeanVector[[g]]=rowMeans(generateDataraw[[g]])
-VarVector[[g]]=apply(generateDataraw[[g]],1,var)
-MOV.post[[g]]=MeanVector[[g]]/VarVector[[g]]
-}
-
-
-### Remove MOV=NA
-generateData=generateDataraw
-for (i in 1:3) generateData[[i]]=generateData[[i]][!is.na(MOV.post[[i]]),]
-print(paste("NA MOV's",sum(is.na(unlist(MOV.post)))))
-#tmpmean=sapply(1:9,function(i)rowMeans(generateData[[i]]))
-#tmpvar=sapply(1:9,function(i)apply(generateData[[i]],1,var))
-#source("plot_functions.R")
-#CheckSimuNg(tmpmean,tmpvar,c(-1,5),c(-1,7))
-
-
-
-
-## DE
-UseName=sapply(1:3, function(i)rownames(generateData[[i]]),simplify=F)
-TrueDE=sapply(1:3, function(i)UseName[[i]][UseName[[i]] %in% rownames(generateData[[i]])[1:DEIsoNumbers[i]]],simplify=F)
-TrueDE.unlist=do.call(c,TrueDE)
-
-TrueDELength=sapply(TrueDE,length)
-
-AtNames_Level=vector("list",4)
-AtLoc=vector("list",3)
-AtFold=vector("list",3)
-names(AtNames_Level)=c(4,6,8,10)
-
-
-for(j in 1:3){
-AtLoc[[j]]=sample(c(1:length(Conditions)), TrueDELength[j], replace=T)
-AtFold[[j]]=sample(c(4,6,8,10),TrueDELength[j], replace=T)
-
-for(i in 1:TrueDELength[j]){
-
-generateData[[j]][(TrueDELength[j]+i),AtLoc[[j]][i]]=generateData[[j]][(TrueDELength[j]+i),AtLoc[[j]][i]]*AtFold[[j]][i]
-AtNames_Level[[as.character(AtFold[[j]][i])]]=c(AtNames_Level[[as.character(AtFold[[j]][i])]],rownames(generateData[[j]])[TrueDELength[j]+i])
-}
-}
-phiuse=sapply(1:3,function(i)PhiInputNg[[i]][UseName[[i]]])
-meanuse=sapply(1:3,function(i)MeanInputNg[[i]][UseName[[i]]])
-
-#generateDataNg=list(generateData[[1]], do.call(rbind,generateData[c(2,4,6,8)]), do.call(rbind,generateData[c(3,5,7,9)]))
-generateDataNg=generateData
-
-#if(OnlyData==T){
-
-OutName=sapply(1:3,function(i)paste("Iso",i,c(1:nrow(generateDataNg[[i]])),sep="_"))
-for(i in 1:3)names(OutName[[i]])=rownames(generateDataNg[[i]])
-OutData=generateDataNg
-for(i in 1:3)rownames(OutData[[i]])=as.vector(OutName[[i]])
-OutTrueDE=as.vector(unlist(OutName)[TrueDE.unlist])
-OutAt=as.vector(unlist(OutName)[AtNames <- Level])
-
-output=list(data=OutData, TrueDE=OutTrueDE, Outliers=OutAt)
-# return(output)
-# }
- }
projects / rsem.git / blobdiff