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A7DA201D113FECD400BF472F /* classifyseqscommand.cpp */,
A7D215C811996C6E00F13F13 /* clearcutcommand.h */,
A7D215C911996C6E00F13F13 /* clearcutcommand.cpp */,
- A7DA2022113FECD400BF472F /* clustercommand.h */,
A7DA2021113FECD400BF472F /* clustercommand.cpp */,
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- A73097BA11A43E1300117C95 /* clustersplitcommand.cpp */,
+ A7DA2022113FECD400BF472F /* clustercommand.h */,
A7DA2025113FECD400BF472F /* collectcommand.cpp */,
A7DA2026113FECD400BF472F /* collectcommand.h */,
A7DA2029113FECD400BF472F /* collectsharedcommand.cpp */,
A76C4A1111876BAF0009460B /* setlogfilecommand.cpp */,
A7DA2110113FECD400BF472F /* sharedcommand.h */,
A7DA210F113FECD400BF472F /* sharedcommand.cpp */,
- A73F163411A1951D0087CA57 /* splitabundcommand.h */,
- A73F163511A1951D0087CA57 /* splitabundcommand.cpp */,
A7DA2155113FECD400BF472F /* summarycommand.h */,
A7DA2154113FECD400BF472F /* summarycommand.cpp */,
A7DA2159113FECD400BF472F /* summarysharedcommand.h */,
A7DA20ED113FECD400BF472F /* readphylip.h */,
A7DA20EE113FECD400BF472F /* readtree.cpp */,
A7DA20EF113FECD400BF472F /* readtree.h */,
- A730977D11A417BE00117C95 /* splitmatrix.h */,
- A730977E11A417BE00117C95 /* splitmatrix.cpp */,
);
name = read;
sourceTree = "<group>";
GCC_WARN_UNUSED_VARIABLE = YES;
LINK_WITH_STANDARD_LIBRARIES = YES;
PREBINDING = NO;
- SDKROOT = "$(DEVELOPER_SDK_DIR)/MacOSX10.5.sdk";
+ SDKROOT = "$(DEVELOPER_SDK_DIR)/MacOSX10.6.sdk";
};
name = Release;
};
return 0;
}
- bool success = 1;
+ int success = 1;
Sequence currSeq(inFASTA);
if(barcodes.size() != 0){
success = stripBarcode(currSeq, group);
-// cout << "here: " << success << endl;
if(success > bdiffs){ trashCode += 'b'; }
else{ currentSeqsDiffs += success; }
}
if(flip){ currSeq.reverseComplement(); } // should go last
if(trashCode.length() == 0){
- currSeq.setAligned(currSeq.getUnaligned()); //this is because of a modification we made to the sequence class to fix a bug. all seqs have an aligned version, which is the version that gets printed.
+ currSeq.setAligned(currSeq.getUnaligned());
currSeq.printSequence(outFASTA);
if(barcodes.size() != 0){
outGroups << currSeq.getName() << '\t' << groupVector[group] << endl;
else{
currSeq.setName(currSeq.getName() + '|' + trashCode);
currSeq.setUnaligned(origSeq);
+ currSeq.setAligned(origSeq);
currSeq.printSequence(scrapFASTA);
}
}
int TrimSeqsCommand::stripBarcode(Sequence& seq, int& group){
try {
+
string rawSequence = seq.getUnaligned();
- bool success = bdiffs + 1; //guilty until proven innocent
+ int success = bdiffs + 1; //guilty until proven innocent
//can you find the barcode
for(map<string,int>::iterator it=barcodes.begin();it!=barcodes.end();it++){
string oligo = it->first;
if(rawSequence.length() < oligo.length()){ //let's just assume that the barcodes are the same length
- success = bdiffs + 1;
- break;
+ success = bdiffs + 10; //if the sequence is shorter than the barcode then bail out
+ break;
}
if(compareDNASeq(oligo, rawSequence.substr(0,oligo.length()))){
// int length = oligo.length();
if(rawSequence.length() < maxLength){ //let's just assume that the barcodes are the same length
- success = bdiffs + 1;
+ success = bdiffs + 10;
break;
}
}
oligo = oligo.substr(0,alnLength);
temp = temp.substr(0,alnLength);
-//
int newStart=0;
int numDiff = countDiffs(oligo, temp);
}
}
- if(minDiff > bdiffs){ success = bdiffs + 1; }
- else if(minCount > 1) { success = bdiffs + 1; }
- else{
+
+ if(minDiff > bdiffs) { success = minDiff; } //no good matches
+ else if(minCount > 1) { success = bdiffs + 100; } //can't tell the difference between multiple barcodes
+ else{ //use the best match
group = minGroup;
- seq.setUnaligned("*" + rawSequence.substr(minPos));
+ seq.setUnaligned(rawSequence.substr(minPos));
success = minDiff;
}
if (alignment != NULL) { delete alignment; }
}
+// cout << success << endl;
+
return success;
}
int TrimSeqsCommand::stripForward(Sequence& seq){
try {
string rawSequence = seq.getUnaligned();
- bool success = pdiffs + 1; //guilty until proven innocent
+ int success = pdiffs + 1; //guilty until proven innocent
//can you find the primer
for(int i=0;i<numFPrimers;i++){
string oligo = forPrimer[i];
if(rawSequence.length() < maxLength){
- success = pdiffs + 1;
+ success = pdiffs + 100;
break;
}
}
oligo = oligo.substr(0,alnLength);
temp = temp.substr(0,alnLength);
-//
+
int newStart=0;
int numDiff = countDiffs(oligo, temp);
if(numDiff < minDiff){
}
}
- if(minDiff > pdiffs){ success = pdiffs + 1; }
- else if(minCount > 1) { success = pdiffs + 1; }
+ if(minDiff > pdiffs) { success = minDiff; }
+ else if(minCount > 1) { success = pdiffs + 10; }
else{
- seq.setUnaligned("*" + rawSequence.substr(minPos));
+ seq.setUnaligned(rawSequence.substr(minPos));
success = minDiff;
}