if (sharedfile != "") { getSubSampleShared(); }
if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { remove(outputNames[i].c_str()); return 0; } }
+
if (listfile != "") { getSubSampleList(); }
if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { remove(outputNames[i].c_str()); return 0; } }
- //if (rabund != "") { getSubSampleRabund(); }
+
+ if (rabundfile != "") { getSubSampleRabund(); }
if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { remove(outputNames[i].c_str()); return 0; } }
- //if (sabundfile != "") { getSubSampleSabund(); }
+
+ if (sabundfile != "") { getSubSampleSabund(); }
if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { remove(outputNames[i].c_str()); return 0; } }
- //if (fastafile != "") { getSubSampleFasta(); }
+
+ if (fastafile != "") { getSubSampleFasta(); }
if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { remove(outputNames[i].c_str()); return 0; } }
}
}
//**********************************************************************************************************************
+int SubSampleCommand::getSubSampleFasta() {
+ try {
+
+ if (namefile != "") { readNames(); } //fills names with all names in namefile.
+ else { getNames(); }//no name file, so get list of names to pick from
+
+ GroupMap* groupMap;
+ if (groupfile != "") {
+
+ groupMap = new GroupMap(groupfile);
+ groupMap->readMap();
+
+ //takes care of user setting groupNames that are invalid or setting groups=all
+ SharedUtil* util = new SharedUtil();
+ util->setGroups(Groups, groupMap->namesOfGroups);
+ delete util;
+
+ //file mismatch quit
+ if (names.size() != groupMap->getNumSeqs()) {
+ m->mothurOut("[ERROR]: your fasta file contains " + toString(names.size()) + " sequences, and your groupfile contains " + toString(groupMap->getNumSeqs()) + ", please correct.");
+ m->mothurOutEndLine();
+ delete groupMap;
+ return 0;
+ }
+ }
+
+ if (m->control_pressed) { return 0; }
+
+ string thisOutputDir = outputDir;
+ if (outputDir == "") { thisOutputDir += m->hasPath(fastafile); }
+ string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(fastafile)) + "subsample" + m->getExtension(fastafile);
+
+ ofstream out;
+ m->openOutputFile(outputFileName, out);
+ outputTypes["fasta"].push_back(outputFileName); outputNames.push_back(outputFileName);
+
+ //make sure that if your picked groups size is not too big
+
+ if (pickedGroups) {
+ int total = 0;
+ for(int i = 0; i < Groups.size(); i++) {
+ total += groupMap->getNumSeqs(Groups[i]);
+ }
+
+ if (size == 0) { //user has not set size, set size = 10% samples size
+ size = int (total * 0.10);
+ }
+
+ if (total < size) {
+ if (size != 0) {
+ m->mothurOut("Your size is too large for the number of groups you selected. Adjusting to " + toString(int (total * 0.10)) + "."); m->mothurOutEndLine();
+ }
+ size = int (total * 0.10);
+ }
+
+ m->mothurOut("Sampling " + toString(size) + " from " + toString(total) + "."); m->mothurOutEndLine();
+ }
+
+ if (size == 0) { //user has not set size, set size = 10% samples size
+ size = int (names.size() * 0.10);
+ }
+
+ int thisSize = names.size();
+ if (size > thisSize) { m->mothurOut("Your fasta file only contains " + toString(thisSize) + " sequences. Setting size to " + toString(thisSize) + "."); m->mothurOutEndLine();
+ size = thisSize;
+ }
+
+ random_shuffle(names.begin(), names.end());
+
+ if (!pickedGroups) { m->mothurOut("Sampling " + toString(size) + " from " + toString(thisSize) + "."); m->mothurOutEndLine(); }
+
+ //randomly select a subset of those names to include in the subsample
+ set<string> subset; //dont want repeat sequence names added
+ for (int j = 0; j < size; j++) {
+
+ if (m->control_pressed) { return 0; }
+
+ //get random sequence to add, making sure we have not already added it
+ bool done = false;
+ int myrand;
+ while (!done) {
+ myrand = (int)((float)(rand()) / (RAND_MAX / (thisSize-1) + 1));
+
+ if (subset.count(names[myrand]) == 0) {
+
+ if (groupfile != "") { //if there is a groupfile given fill in group info
+ string group = groupMap->getGroup(names[myrand]);
+ if (group == "not found") { m->mothurOut("[ERROR]: " + names[myrand] + " is not in your groupfile. please correct."); m->mothurOutEndLine(); group = "NOTFOUND"; }
+
+ if (pickedGroups) { //if hte user picked groups, we only want to keep the names of sequences from those groups
+ if (m->inUsersGroups(group, Groups)) {
+ subset.insert(names[myrand]); break;
+ }
+ }else{
+ subset.insert(names[myrand]); break;
+ }
+ }else{ //save everyone, group
+ subset.insert(names[myrand]); break;
+ }
+ }
+ }
+ }
+
+ //read through fasta file outputting only the names on the subsample list
+ ifstream in;
+ m->openInputFile(fastafile, in);
+
+ string thisname;
+ int count = 0;
+ map<string, vector<string> >::iterator itNameMap;
+
+ while(!in.eof()){
+
+ if (m->control_pressed) { in.close(); out.close(); return 0; }
+
+ Sequence currSeq(in);
+ thisname = currSeq.getName();
+
+ if (thisname != "") {
+
+ //does the subset contain a sequence that this sequence represents
+ itNameMap = nameMap.find(thisname);
+ if (itNameMap != nameMap.end()) {
+ vector<string> nameRepresents = itNameMap->second;
+
+ for (int i = 0; i < nameRepresents.size(); i++){
+ if (subset.count(nameRepresents[i]) != 0) {
+ out << ">" << nameRepresents[i] << endl << currSeq.getAligned() << endl;
+ count++;
+ }
+ }
+ }else{
+ m->mothurOut("[ERROR]: " + thisname + " is not in your namefile, please correct."); m->mothurOutEndLine();
+ }
+ }
+ m->gobble(in);
+ }
+ in.close();
+ out.close();
+
+ if (count != subset.size()) {
+ m->mothurOut("[ERROR]: The subset selected contained " + toString(subset.size()) + " sequences, but I only found " + toString(count) + " of those in the fastafile."); m->mothurOutEndLine();
+ }
+
+ //if a groupfile is provided read through the group file only outputting the names on the subsample list
+ if (groupfile != "") {
+
+ string groupOutputDir = outputDir;
+ if (outputDir == "") { groupOutputDir += m->hasPath(groupfile); }
+ string groupOutputFileName = groupOutputDir + m->getRootName(m->getSimpleName(groupfile)) + "subsample" + m->getExtension(groupfile);
+
+ ofstream outGroup;
+ m->openOutputFile(groupOutputFileName, outGroup);
+ outputTypes["group"].push_back(groupOutputFileName); outputNames.push_back(groupOutputFileName);
+
+ ifstream inGroup;
+ m->openInputFile(groupfile, inGroup);
+ string name, group;
+
+ while(!inGroup.eof()){
+
+ if (m->control_pressed) { inGroup.close(); outGroup.close(); return 0; }
+
+ inGroup >> name; m->gobble(inGroup); //read from first column
+ inGroup >> group; //read from second column
+
+ //if this name is in the accnos file
+ if (subset.count(name) != 0) {
+ outGroup << name << '\t' << group << endl;
+ subset.erase(name);
+ }
+
+ m->gobble(inGroup);
+ }
+ inGroup.close();
+ outGroup.close();
+
+ //sanity check
+ if (subset.size() != 0) {
+ m->mothurOut("Your groupfile does not match your fasta file."); m->mothurOutEndLine();
+ for (set<string>::iterator it = subset.begin(); it != subset.end(); it++) {
+ m->mothurOut("[ERROR]: " + *it + " is missing from your groupfile."); m->mothurOutEndLine();
+ }
+ }
+ }
+
+ return 0;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "SubSampleCommand", "getSubSampleFasta");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+int SubSampleCommand::getNames() {
+ try {
+
+ ifstream in;
+ m->openInputFile(fastafile, in);
+
+ string thisname;
+ while(!in.eof()){
+
+ if (m->control_pressed) { in.close(); return 0; }
+
+ Sequence currSeq(in);
+ thisname = currSeq.getName();
+
+ if (thisname != "") {
+ vector<string> temp; temp.push_back(thisname);
+ nameMap[thisname] = temp;
+ names.push_back(thisname);
+ }
+ m->gobble(in);
+ }
+ in.close();
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "SubSampleCommand", "getNames");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+int SubSampleCommand::readNames() {
+ try {
+
+ ifstream in;
+ m->openInputFile(namefile, in);
+
+ string thisname, repnames;
+ map<string, vector<string> >::iterator it;
+
+ while(!in.eof()){
+
+ if (m->control_pressed) { in.close(); return 0; }
+
+ in >> thisname; m->gobble(in); //read from first column
+ in >> repnames; //read from second column
+
+ it = nameMap.find(thisname);
+ if (it == nameMap.end()) {
+
+ vector<string> splitRepNames;
+ m->splitAtComma(repnames, splitRepNames);
+
+ nameMap[thisname] = splitRepNames;
+ for (int i = 0; i < splitRepNames.size(); i++) { names.push_back(splitRepNames[i]); }
+
+ }else{ m->mothurOut(thisname + " is already in namesfile. I will use first definition."); m->mothurOutEndLine(); }
+
+ m->gobble(in);
+ }
+ in.close();
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "SubSampleCommand", "readNames");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
int SubSampleCommand::getSubSampleShared() {
try {
set<string> processedLabels;
set<string> userLabels = labels;
+ if (size == 0) { //user has not set size, set size = smallest samples size
+ size = lookup[0]->getNumSeqs();
+ for (int i = 1; i < lookup.size(); i++) {
+ int thisSize = lookup[i]->getNumSeqs();
+
+ if (thisSize < size) { size = thisSize; }
+ }
+ }
+
+ m->mothurOut("Sampling " + toString(size) + " from " + toString(lookup[0]->getNumSeqs()) + "."); m->mothurOutEndLine();
//as long as you are not at the end of the file or done wih the lines you want
while((lookup[0] != NULL) && ((allLines == 1) || (userLabels.size() != 0))) {
- if (m->control_pressed) { delete input; out.close(); return 0; }
+ if (m->control_pressed) { delete input; for (int i = 0; i < lookup.size(); i++) { delete lookup[i]; lookup[i] = NULL; } out.close(); return 0; }
if(allLines == 1 || labels.count(lookup[0]->getLabel()) == 1){
int SubSampleCommand::processShared(vector<SharedRAbundVector*>& thislookup, ofstream& out) {
try {
- //if (pickedGroups) { eliminateZeroOTUS(thislookup); }
-
- if (size == 0) { //user has not set size, set size = smallest samples size
- size = thislookup[0]->getNumSeqs();
- for (int i = 1; i < thislookup.size(); i++) {
- int thisSize = thislookup[i]->getNumSeqs();
-
- if (thisSize < size) { size = thisSize; }
- }
- }
-
int numBins = thislookup[0]->getNumBins();
for (int i = 0; i < thislookup.size(); i++) {
int thisSize = thislookup[i]->getNumSeqs();
m->openOutputFile(outputFileName, out);
outputTypes["list"].push_back(outputFileName); outputNames.push_back(outputFileName);
- InputData* input;
- //if you have a groupfile you want to read a shared list
+ InputData* input = new InputData(listfile, "list");
+ ListVector* list = input->getListVector();
+ string lastLabel = list->getLabel();
+
+ //if the users enters label "0.06" and there is no "0.06" in their file use the next lowest label.
+ set<string> processedLabels;
+ set<string> userLabels = labels;
+
+ ofstream outGroup;
+ GroupMap* groupMap;
if (groupfile != "") {
- GroupMap* groupMap = new GroupMap(groupfile);
+ groupMap = new GroupMap(groupfile);
groupMap->readMap();
//takes care of user setting groupNames that are invalid or setting groups=all
if (outputDir == "") { groupOutputDir += m->hasPath(groupfile); }
string groupOutputFileName = groupOutputDir + m->getRootName(m->getSimpleName(groupfile)) + "subsample" + m->getExtension(groupfile);
- ofstream outGroup;
m->openOutputFile(groupOutputFileName, outGroup);
outputTypes["group"].push_back(groupOutputFileName); outputNames.push_back(groupOutputFileName);
- globaldata->setGroupFile(groupfile); //shared list needs this
-
- input = new InputData(listfile, "list");
- ListVector* list = input->getListVector();
- string lastLabel = list->getLabel();
-
- //if the users enters label "0.06" and there is no "0.06" in their file use the next lowest label.
- set<string> processedLabels;
- set<string> userLabels = labels;
-
//file mismatch quit
if (list->getNumSeqs() != groupMap->getNumSeqs()) {
m->mothurOut("[ERROR]: your list file contains " + toString(list->getNumSeqs()) + " sequences, and your groupfile contains " + toString(groupMap->getNumSeqs()) + ", please correct.");
out.close();
outGroup.close();
return 0;
+ }
+ }
+
+ //make sure that if your picked groups size is not too big
+ if (pickedGroups) {
+ int total = 0;
+ for(int i = 0; i < Groups.size(); i++) {
+ total += groupMap->getNumSeqs(Groups[i]);
}
- //as long as you are not at the end of the file or done wih the lines you want
- while((list != NULL) && ((allLines == 1) || (userLabels.size() != 0))) {
-
- if (m->control_pressed) { delete list; delete input; delete groupMap; out.close(); outGroup.close(); return 0; }
-
- if(allLines == 1 || labels.count(list->getLabel()) == 1){
-
- m->mothurOut(list->getLabel()); m->mothurOutEndLine();
-
- processListGroup(list, groupMap, out, outGroup);
+ if (size == 0) { //user has not set size, set size = 10% samples size
+ size = int (total * 0.10);
+ }
+
+ if (total < size) {
+ m->mothurOut("Your size is too large for the number of groups you selected. Adjusting to " + toString(int (total * 0.10)) + "."); m->mothurOutEndLine();
+ size = int (total * 0.10);
+ }
+
+ m->mothurOut("Sampling " + toString(size) + " from " + toString(total) + "."); m->mothurOutEndLine();
+ }else{
+
+ if (size == 0) { //user has not set size, set size = 10% samples size
+ size = int (list->getNumSeqs() * 0.10);
+ }
+
+ int thisSize = list->getNumSeqs();
+ if (size > thisSize) { m->mothurOut("Your list file only contains " + toString(thisSize) + " sequences. Setting size to " + toString(thisSize) + "."); m->mothurOutEndLine();
+ size = thisSize;
+ }
+
+ m->mothurOut("Sampling " + toString(size) + " from " + toString(list->getNumSeqs()) + "."); m->mothurOutEndLine();
+ }
+
+
+ for (int i = 0; i < list->getNumBins(); i++) {
+ string binnames = list->get(i);
+
+ //parse names
+ string individual = "";
+ int length = binnames.length();
+ for(int j=0;j<length;j++){
+ if(binnames[j] == ','){
- processedLabels.insert(list->getLabel());
- userLabels.erase(list->getLabel());
+ if (groupfile != "") { //if there is a groupfile given fill in group info
+ string group = groupMap->getGroup(individual);
+ if (group == "not found") { m->mothurOut("[ERROR]: " + individual + " is not in your groupfile. please correct."); m->mothurOutEndLine(); group = "NOTFOUND"; }
+
+ if (pickedGroups) { //if hte user picked groups, we only want to keep the names of sequences from those groups
+ if (m->inUsersGroups(group, Groups)) {
+ names.push_back(individual);
+ }
+ }else{
+ names.push_back(individual);
+ }
+ }else{ //save everyone, group
+ names.push_back(individual);
+ }
+ individual = "";
}
-
- if ((m->anyLabelsToProcess(list->getLabel(), userLabels, "") == true) && (processedLabels.count(lastLabel) != 1)) {
- string saveLabel = list->getLabel();
-
- delete list;
-
- list = input->getListVector(lastLabel);
- m->mothurOut(list->getLabel()); m->mothurOutEndLine();
-
- processListGroup(list, groupMap, out, outGroup);
-
- processedLabels.insert(list->getLabel());
- userLabels.erase(list->getLabel());
-
- //restore real lastlabel to save below
- list->setLabel(saveLabel);
+ else{
+ individual += binnames[j];
}
+ }
+ //save last name
+ if (groupfile != "") { //if there is a groupfile given fill in group info
+ string group = groupMap->getGroup(individual);
+ if (group == "not found") { m->mothurOut("[ERROR]: " + individual + " is not in your groupfile. please correct."); m->mothurOutEndLine(); group = "NOTFOUND"; }
- lastLabel = list->getLabel();
+ if (pickedGroups) { //if hte user picked groups, we only want to keep the names of sequences from those groups
+ if (m->inUsersGroups(group, Groups)) {
+ names.push_back(individual);
+ }
+ }else{
+ names.push_back(individual);
+ }
+ }else{ //save everyone, group
+ names.push_back(individual);
+ }
+ }
+
+ random_shuffle(names.begin(), names.end());
+
+ //randomly select a subset of those names to include in the subsample
+ set<string> subset; //dont want repeat sequence names added
+ for (int j = 0; j < size; j++) {
+
+ if (m->control_pressed) { break; }
+
+ //get random sequence to add, making sure we have not already added it
+ bool done = false;
+ int myrand;
+ while (!done) {
+ myrand = (int)((float)(rand()) / (RAND_MAX / (names.size()-1) + 1));
- delete list; list = NULL;
+ if (subset.count(names[myrand]) == 0) { subset.insert(names[myrand]); break; }
+ }
+ }
+
+ if (groupfile != "") {
+ //write out new groupfile
+ for (set<string>::iterator it = subset.begin(); it != subset.end(); it++) {
+ string group = groupMap->getGroup(*it);
+ if (group == "not found") { group = "NOTFOUND"; }
- //get next line to process
- list = input->getListVector();
+ outGroup << *it << '\t' << group << endl;
}
+ outGroup.close(); delete groupMap;
+ }
+
+
+ //as long as you are not at the end of the file or done wih the lines you want
+ while((list != NULL) && ((allLines == 1) || (userLabels.size() != 0))) {
+ if (m->control_pressed) { delete list; delete input; out.close(); return 0; }
- if (m->control_pressed) { if (list != NULL) { delete list; } delete input; delete groupMap; out.close(); outGroup.close(); return 0; }
-
- //output error messages about any remaining user labels
- set<string>::iterator it;
- bool needToRun = false;
- for (it = userLabels.begin(); it != userLabels.end(); it++) {
- m->mothurOut("Your file does not include the label " + *it);
- if (processedLabels.count(lastLabel) != 1) {
- m->mothurOut(". I will use " + lastLabel + "."); m->mothurOutEndLine();
- needToRun = true;
- }else {
- m->mothurOut(". Please refer to " + lastLabel + "."); m->mothurOutEndLine();
- }
+ if(allLines == 1 || labels.count(list->getLabel()) == 1){
+
+ m->mothurOut(list->getLabel()); m->mothurOutEndLine();
+
+ processList(list, out, subset);
+
+ processedLabels.insert(list->getLabel());
+ userLabels.erase(list->getLabel());
}
- //run last label if you need to
- if (needToRun == true) {
- if (list != NULL) { delete list; }
+ if ((m->anyLabelsToProcess(list->getLabel(), userLabels, "") == true) && (processedLabels.count(lastLabel) != 1)) {
+ string saveLabel = list->getLabel();
- list = input->getListVector(lastLabel);
+ delete list;
+ list = input->getListVector(lastLabel);
m->mothurOut(list->getLabel()); m->mothurOutEndLine();
- processListGroup(list, groupMap, out, outGroup);
+ processList(list, out, subset);
+
+ processedLabels.insert(list->getLabel());
+ userLabels.erase(list->getLabel());
- delete list; list = NULL;
+ //restore real lastlabel to save below
+ list->setLabel(saveLabel);
}
- out.close(); outGroup.close();
- if (list != NULL) { delete list; }
- delete groupMap;
+ lastLabel = list->getLabel();
+
+ delete list; list = NULL;
- }else {
- //need to complete
+ //get next line to process
+ list = input->getListVector();
}
+ if (m->control_pressed) { if (list != NULL) { delete list; } delete input; out.close(); return 0; }
+
+ //output error messages about any remaining user labels
+ set<string>::iterator it;
+ bool needToRun = false;
+ for (it = userLabels.begin(); it != userLabels.end(); it++) {
+ m->mothurOut("Your file does not include the label " + *it);
+ if (processedLabels.count(lastLabel) != 1) {
+ m->mothurOut(". I will use " + lastLabel + "."); m->mothurOutEndLine();
+ needToRun = true;
+ }else {
+ m->mothurOut(". Please refer to " + lastLabel + "."); m->mothurOutEndLine();
+ }
+ }
+
+ //run last label if you need to
+ if (needToRun == true) {
+ if (list != NULL) { delete list; }
+
+ list = input->getListVector(lastLabel);
+
+ m->mothurOut(list->getLabel()); m->mothurOutEndLine();
+
+ processList(list, out, subset);
+
+ delete list; list = NULL;
+ }
+
+ out.close();
+ if (list != NULL) { delete list; }
delete input;
return 0;
}
}
//**********************************************************************************************************************
-int SubSampleCommand::processListGroup(ListVector*& list, GroupMap*& groupMap, ofstream& out, ofstream& outGroup) {
+int SubSampleCommand::processList(ListVector*& list, ofstream& out, set<string>& subset) {
try {
- if (size == 0) { //user has not set size, set size = 10% samples size
- size = int (list->getNumSeqs() * 0.10);
- }
-
int numBins = list->getNumBins();
- int thisSize = list->getNumSeqs();
-
- if (size > thisSize) { m->mothurOut("Your list file only contains " + toString(thisSize) + " sequences. Setting size to " + toString(thisSize) + "."); m->mothurOutEndLine();
- size = thisSize;
- }
+
+ ListVector* temp = new ListVector();
+ temp->setLabel(list->getLabel());
- vector<nameToBin> seqs;
for (int i = 0; i < numBins; i++) {
- string names = list->get(i);
+
+ if (m->control_pressed) { break; }
+
+ string binnames = list->get(i);
//parse names
string individual = "";
- int length = names.length();
+ string newNames = "";
+ int length = binnames.length();
for(int j=0;j<length;j++){
- if(names[j] == ','){
- nameToBin temp(individual, i);
- seqs.push_back(temp);
+ if(binnames[j] == ','){
+ if (subset.count(individual) != 0) { newNames += individual + ","; }
individual = "";
- }
- else{
- individual += names[j];
+ }else{
+ individual += binnames[j];
}
}
- nameToBin temp(individual, i);
- seqs.push_back(temp);
+ if (subset.count(individual) != 0) { newNames += individual; }
+
+
+ //if there are names in this bin add to new list
+ if (newNames != "") {
+ newNames = newNames.substr(0, newNames.length()-1); //rip off extra comma
+ temp->push_back(newNames);
+ }
}
-
- ListVector* temp = new ListVector(numBins);
- temp->setLabel(list->getLabel());
-
- delete list;
+ delete list;
list = temp;
- set<int> alreadySelected; //dont want repeat sequence names added
- alreadySelected.insert(-1);
- for (int j = 0; j < size; j++) {
+ if (m->control_pressed) { return 0; }
+
+ list->print(out);
+
+ return 0;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "SubSampleCommand", "processList");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+int SubSampleCommand::getSubSampleRabund() {
+ try {
+
+ string thisOutputDir = outputDir;
+ if (outputDir == "") { thisOutputDir += m->hasPath(rabundfile); }
+ string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(rabundfile)) + "subsample" + m->getExtension(rabundfile);
+
+ ofstream out;
+ m->openOutputFile(outputFileName, out);
+ outputTypes["rabund"].push_back(outputFileName); outputNames.push_back(outputFileName);
+
+ InputData* input = new InputData(rabundfile, "rabund");
+ RAbundVector* rabund = input->getRAbundVector();
+ string lastLabel = rabund->getLabel();
+
+ //if the users enters label "0.06" and there is no "0.06" in their file use the next lowest label.
+ set<string> processedLabels;
+ set<string> userLabels = labels;
+
+ if (size == 0) { //user has not set size, set size = 10%
+ size = int((rabund->getNumSeqs()) * 0.10);
+ }
+
+ m->mothurOut("Sampling " + toString(size) + " from " + toString(rabund->getNumSeqs()) + "."); m->mothurOutEndLine();
+
+ //as long as you are not at the end of the file or done wih the lines you want
+ while((rabund != NULL) && ((allLines == 1) || (userLabels.size() != 0))) {
+ if (m->control_pressed) { delete input; delete rabund; out.close(); return 0; }
- if (m->control_pressed) { return 0; }
+ if(allLines == 1 || labels.count(rabund->getLabel()) == 1){
+
+ m->mothurOut(rabund->getLabel()); m->mothurOutEndLine();
+
+ processRabund(rabund, out);
+
+ processedLabels.insert(rabund->getLabel());
+ userLabels.erase(rabund->getLabel());
+ }
- //get random sequence to add, making sure we have not already added it
- int myrand = -1;
- while (alreadySelected.count(myrand) != 0) {
- myrand = (int)((float)(rand()) / (RAND_MAX / (thisSize-1) + 1));
+ if ((m->anyLabelsToProcess(rabund->getLabel(), userLabels, "") == true) && (processedLabels.count(lastLabel) != 1)) {
+ string saveLabel = rabund->getLabel();
+
+ delete rabund;
+
+ rabund = input->getRAbundVector(lastLabel);
+ m->mothurOut(rabund->getLabel()); m->mothurOutEndLine();
+
+ processRabund(rabund, out);
+
+ processedLabels.insert(rabund->getLabel());
+ userLabels.erase(rabund->getLabel());
+
+ //restore real lastlabel to save below
+ rabund->setLabel(saveLabel);
+ }
+
+ lastLabel = rabund->getLabel();
+
+ //prevent memory leak
+ delete rabund; rabund = NULL;
+
+ //get next line to process
+ rabund = input->getRAbundVector();
+ }
+
+
+ if (m->control_pressed) { out.close(); return 0; }
+
+ //output error messages about any remaining user labels
+ set<string>::iterator it;
+ bool needToRun = false;
+ for (it = userLabels.begin(); it != userLabels.end(); it++) {
+ m->mothurOut("Your file does not include the label " + *it);
+ if (processedLabels.count(lastLabel) != 1) {
+ m->mothurOut(". I will use " + lastLabel + "."); m->mothurOutEndLine();
+ needToRun = true;
+ }else {
+ m->mothurOut(". Please refer to " + lastLabel + "."); m->mothurOutEndLine();
}
- alreadySelected.insert(myrand);
+ }
+
+ //run last label if you need to
+ if (needToRun == true) {
+ if (rabund != NULL) { delete rabund; }
- //update new list
- string oldNames = temp->get(seqs[myrand].bin);
- if (oldNames == "") { oldNames += seqs[myrand].name; }
- else { oldNames += "," + seqs[myrand].name; }
+ rabund = input->getRAbundVector(lastLabel);
- temp->set(seqs[myrand].bin, oldNames);
+ m->mothurOut(rabund->getLabel()); m->mothurOutEndLine();
- //update group file
- string group = groupMap->getGroup(seqs[myrand].name);
- if (group == "not found") { m->mothurOut("[ERROR]: " + seqs[myrand].name + " is not in your groupfile. please correct."); m->mothurOutEndLine(); group = "NOTFOUND"; }
+ processRabund(rabund, out);
- outGroup << seqs[myrand].name << '\t' << group << endl;
- }
-
+ delete rabund;
+ }
+
+ delete input;
+ out.close();
+
+ return 0;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "SubSampleCommand", "getSubSampleRabund");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+int SubSampleCommand::processRabund(RAbundVector*& rabund, ofstream& out) {
+ try {
+
+ int numBins = rabund->getNumBins();
+ int thisSize = rabund->getNumSeqs();
+
+ if (thisSize != size) {
+
+ OrderVector* order = new OrderVector();
+ for(int p=0;p<numBins;p++){
+ for(int j=0;j<rabund->get(p);j++){
+ order->push_back(p);
+ }
+ }
+ random_shuffle(order->begin(), order->end());
+
+ RAbundVector* temp = new RAbundVector(numBins);
+ temp->setLabel(rabund->getLabel());
+
+ delete rabund;
+ rabund = temp;
+
+ for (int j = 0; j < size; j++) {
+
+ if (m->control_pressed) { delete order; return 0; }
+
+ //get random number to sample from order between 0 and thisSize-1.
+ int myrand = (int)((float)(rand()) / (RAND_MAX / (thisSize-1) + 1));
+
+ int bin = order->get(myrand);
+
+ int abund = rabund->get(bin);
+ rabund->set(bin, (abund+1));
+ }
+
+ delete order;
+ }
+
if (m->control_pressed) { return 0; }
- list->print(out);
+ rabund->print(out);
return 0;
}
catch(exception& e) {
- m->errorOut(e, "SubSampleCommand", "processListGroup");
+ m->errorOut(e, "SubSampleCommand", "processRabund");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+int SubSampleCommand::getSubSampleSabund() {
+ try {
+
+ string thisOutputDir = outputDir;
+ if (outputDir == "") { thisOutputDir += m->hasPath(sabundfile); }
+ string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(sabundfile)) + "subsample" + m->getExtension(sabundfile);
+
+ ofstream out;
+ m->openOutputFile(outputFileName, out);
+ outputTypes["sabund"].push_back(outputFileName); outputNames.push_back(outputFileName);
+
+ InputData* input = new InputData(sabundfile, "sabund");
+ SAbundVector* sabund = input->getSAbundVector();
+ string lastLabel = sabund->getLabel();
+
+ //if the users enters label "0.06" and there is no "0.06" in their file use the next lowest label.
+ set<string> processedLabels;
+ set<string> userLabels = labels;
+
+ if (size == 0) { //user has not set size, set size = 10%
+ size = int((sabund->getNumSeqs()) * 0.10);
+ }
+
+ m->mothurOut("Sampling " + toString(size) + " from " + toString(sabund->getNumSeqs()) + "."); m->mothurOutEndLine();
+
+ //as long as you are not at the end of the file or done wih the lines you want
+ while((sabund != NULL) && ((allLines == 1) || (userLabels.size() != 0))) {
+ if (m->control_pressed) { delete input; delete sabund; out.close(); return 0; }
+
+ if(allLines == 1 || labels.count(sabund->getLabel()) == 1){
+
+ m->mothurOut(sabund->getLabel()); m->mothurOutEndLine();
+
+ processSabund(sabund, out);
+
+ processedLabels.insert(sabund->getLabel());
+ userLabels.erase(sabund->getLabel());
+ }
+
+ if ((m->anyLabelsToProcess(sabund->getLabel(), userLabels, "") == true) && (processedLabels.count(lastLabel) != 1)) {
+ string saveLabel = sabund->getLabel();
+
+ delete sabund;
+
+ sabund = input->getSAbundVector(lastLabel);
+ m->mothurOut(sabund->getLabel()); m->mothurOutEndLine();
+
+ processSabund(sabund, out);
+
+ processedLabels.insert(sabund->getLabel());
+ userLabels.erase(sabund->getLabel());
+
+ //restore real lastlabel to save below
+ sabund->setLabel(saveLabel);
+ }
+
+ lastLabel = sabund->getLabel();
+
+ //prevent memory leak
+ delete sabund; sabund = NULL;
+
+ //get next line to process
+ sabund = input->getSAbundVector();
+ }
+
+
+ if (m->control_pressed) { out.close(); return 0; }
+
+ //output error messages about any remaining user labels
+ set<string>::iterator it;
+ bool needToRun = false;
+ for (it = userLabels.begin(); it != userLabels.end(); it++) {
+ m->mothurOut("Your file does not include the label " + *it);
+ if (processedLabels.count(lastLabel) != 1) {
+ m->mothurOut(". I will use " + lastLabel + "."); m->mothurOutEndLine();
+ needToRun = true;
+ }else {
+ m->mothurOut(". Please refer to " + lastLabel + "."); m->mothurOutEndLine();
+ }
+ }
+
+ //run last label if you need to
+ if (needToRun == true) {
+ if (sabund != NULL) { delete sabund; }
+
+ sabund = input->getSAbundVector(lastLabel);
+
+ m->mothurOut(sabund->getLabel()); m->mothurOutEndLine();
+
+ processSabund(sabund, out);
+
+ delete sabund;
+ }
+
+ delete input;
+ out.close();
+
+ return 0;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "SubSampleCommand", "getSubSampleSabund");
exit(1);
}
}
//**********************************************************************************************************************
+int SubSampleCommand::processSabund(SAbundVector*& sabund, ofstream& out) {
+ try {
+
+ RAbundVector* rabund = new RAbundVector();
+ *rabund = sabund->getRAbundVector();
+
+ int numBins = rabund->getNumBins();
+ int thisSize = rabund->getNumSeqs();
+
+ if (thisSize != size) {
+
+ OrderVector* order = new OrderVector();
+ for(int p=0;p<numBins;p++){
+ for(int j=0;j<rabund->get(p);j++){
+ order->push_back(p);
+ }
+ }
+ random_shuffle(order->begin(), order->end());
+
+ RAbundVector* temp = new RAbundVector(numBins);
+ temp->setLabel(rabund->getLabel());
+
+ delete rabund;
+ rabund = temp;
+
+ for (int j = 0; j < size; j++) {
+
+ if (m->control_pressed) { delete order; return 0; }
+
+ //get random number to sample from order between 0 and thisSize-1.
+ int myrand = (int)((float)(rand()) / (RAND_MAX / (thisSize-1) + 1));
+
+ int bin = order->get(myrand);
+
+ int abund = rabund->get(bin);
+ rabund->set(bin, (abund+1));
+ }
+
+ delete order;
+ }
+
+ if (m->control_pressed) { return 0; }
+
+ delete sabund;
+ sabund = new SAbundVector();
+ *sabund = rabund->getSAbundVector();
+ delete rabund;
+
+ sabund->print(out);
+
+ return 0;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "SubSampleCommand", "processSabund");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
int SubSampleCommand::eliminateZeroOTUS(vector<SharedRAbundVector*>& thislookup) {
try {
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