]> git.donarmstrong.com Git - mothur.git/blobdiff - tree.cpp
added modify names parameter to set.dir
[mothur.git] / tree.cpp
index 731eb64d93f95b485f8607308e3e88345138813c..745893471e065abee12e7752b91c71bb37c1a635 100644 (file)
--- a/tree.cpp
+++ b/tree.cpp
@@ -9,32 +9,84 @@
 
 #include "tree.h"
 
-
 /*****************************************************************/
-Tree::Tree() {
+Tree::Tree(int num, CountTable* t) : ct(t) {
        try {
-               globaldata = GlobalData::getInstance();
+               m = MothurOut::getInstance();
                
-               if (globaldata->runParse == true) {  parseTreeFile();  globaldata->runParse = false;  }
-//for(int i = 0; i <   globaldata->Treenames.size(); i++) { cout << i << '\t' << globaldata->Treenames[i] << endl;  }  
-               numLeaves = globaldata->Treenames.size();
+               numLeaves = num;  
                numNodes = 2*numLeaves - 1;
-               
+        
                tree.resize(numNodes);
+       }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "Tree - numNodes");
+               exit(1);
+       }
+}
+/*****************************************************************/
+Tree::Tree(string g) { //do not use tree generated by this its just to extract the treenames, its a chicken before the egg thing that needs to be revisited.
+       try {
+               m = MothurOut::getInstance();
+               parseTreeFile();  m->runParse = false;  
+       }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "Tree - just parse");
+               exit(1);
+       }
+}
+/*****************************************************************/
+Tree::Tree(CountTable* t) : ct(t) {
+       try {
+               m = MothurOut::getInstance();
+               
+               if (m->runParse == true) {  parseTreeFile();  m->runParse = false;  }
 
+               numLeaves = m->Treenames.size();
+               numNodes = 2*numLeaves - 1;
+               
+               tree.resize(numNodes);
+                       
+               //initialize groupNodeInfo
+        vector<string> namesOfGroups = ct->getNamesOfGroups();
+               for (int i = 0; i < namesOfGroups.size(); i++) {  groupNodeInfo[namesOfGroups[i]].resize(0);  }
+               
                //initialize tree with correct number of nodes, name and group info.
                for (int i = 0; i < numNodes; i++) {
                        //initialize leaf nodes
                        if (i <= (numLeaves-1)) {
-                               tree[i].setName(globaldata->Treenames[i]);
-                               vector<string> tempGroups; tempGroups.push_back(globaldata->gTreemap->getGroup(globaldata->Treenames[i]));
-                               tree[i].setGroup(tempGroups);
-                               //set pcount and pGroup for groupname to 1.
-                               tree[i].pcount[globaldata->gTreemap->getGroup(globaldata->Treenames[i])] = 1;
-                               tree[i].pGroups[globaldata->gTreemap->getGroup(globaldata->Treenames[i])] = 1;
-                               //Treemap knows name, group and index to speed up search
-                               globaldata->gTreemap->setIndex(globaldata->Treenames[i], i);
-       
+                               tree[i].setName(m->Treenames[i]);
+                               
+                               //save group info
+                int maxPars = 1;
+                               vector<string> group;
+                vector<int> counts = ct->getGroupCounts(m->Treenames[i]);
+                               for (int j = 0; j < namesOfGroups.size(); j++) {  
+                    if (counts[j] != 0) { //you have seqs from this group
+                        groupNodeInfo[namesOfGroups[j]].push_back(i);
+                        group.push_back(namesOfGroups[j]);
+                        tree[i].pGroups[namesOfGroups[j]] = counts[j];
+                        tree[i].pcount[namesOfGroups[j]] = counts[j];
+                        //keep highest group
+                                               if(counts[j] > maxPars){ maxPars = counts[j]; }
+                    }  
+                }
+                               tree[i].setGroup(group);
+                               setIndex(m->Treenames[i], i);
+                
+                if (maxPars > 1) { //then we have some more dominant groups
+                                       //erase all the groups that are less than maxPars because you found a more dominant group.
+                                       for(it=tree[i].pGroups.begin();it!=tree[i].pGroups.end();){
+                                               if(it->second < maxPars){
+                                                       tree[i].pGroups.erase(it++);
+                                               }else { it++; }
+                                       }
+                                       //set one remaining groups to 1
+                                       for(it=tree[i].pGroups.begin();it!=tree[i].pGroups.end();it++){
+                                               tree[i].pGroups[it->first] = 1;
+                                       }
+                               }//end if
+                
                        //intialize non leaf nodes
                        }else if (i > (numLeaves-1)) {
                                tree[i].setName("");
@@ -42,17 +94,145 @@ Tree::Tree() {
                                tree[i].setGroup(tempGroups);
                        }
                }
+               
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "Tree");
+               m->errorOut(e, "Tree", "Tree");
                exit(1);
        }
 }
+/*****************************************************************/
+Tree::Tree(CountTable* t, vector< vector<double> >& sims) : ct(t) {
+       try {
+               m = MothurOut::getInstance();
+               
+               if (m->runParse == true) {  parseTreeFile();  m->runParse = false;  }
+               numLeaves = m->Treenames.size();
+               numNodes = 2*numLeaves - 1;
+               
+               tree.resize(numNodes);
+        
+               //initialize groupNodeInfo
+        vector<string> namesOfGroups = ct->getNamesOfGroups();
+               for (int i = 0; i < namesOfGroups.size(); i++) {  groupNodeInfo[namesOfGroups[i]].resize(0);  }
+               
+               //initialize tree with correct number of nodes, name and group info.
+               for (int i = 0; i < numNodes; i++) {
+                       //initialize leaf nodes
+                       if (i <= (numLeaves-1)) {
+                               tree[i].setName(m->Treenames[i]);
+                               
+                               //save group info
+                int maxPars = 1;
+                               vector<string> group;
+                vector<int> counts = ct->getGroupCounts(m->Treenames[i]);
+                               for (int j = 0; j < namesOfGroups.size(); j++) {  
+                    if (counts[j] != 0) { //you have seqs from this group
+                        groupNodeInfo[namesOfGroups[j]].push_back(i);
+                        group.push_back(namesOfGroups[j]);
+                        tree[i].pGroups[namesOfGroups[j]] = counts[j];
+                        tree[i].pcount[namesOfGroups[j]] = counts[j];
+                        //keep highest group
+                                               if(counts[j] > maxPars){ maxPars = counts[j]; }
+                    }  
+                }
+                               tree[i].setGroup(group);
+                               setIndex(m->Treenames[i], i);
+                
+                if (maxPars > 1) { //then we have some more dominant groups
+                                       //erase all the groups that are less than maxPars because you found a more dominant group.
+                                       for(it=tree[i].pGroups.begin();it!=tree[i].pGroups.end();){
+                                               if(it->second < maxPars){
+                                                       tree[i].pGroups.erase(it++);
+                                               }else { it++; }
+                                       }
+                                       //set one remaining groups to 1
+                                       for(it=tree[i].pGroups.begin();it!=tree[i].pGroups.end();it++){
+                                               tree[i].pGroups[it->first] = 1;
+                                       }
+                               }//end if
+                
+                //intialize non leaf nodes
+                       }else if (i > (numLeaves-1)) {
+                               tree[i].setName("");
+                               vector<string> tempGroups;
+                               tree[i].setGroup(tempGroups);
+                       }
+               }
 
+        
+        //build tree from matrix
+        //initialize indexes
+        map<int, int> thisIndexes;  //maps row in simMatrix to vector index in the tree
+        for (int g = 0; g < numLeaves; g++) {  thisIndexes[g] = g;     }
+               
+               //do merges and create tree structure by setting parents and children
+               //there are numGroups - 1 merges to do
+               for (int i = 0; i < (numLeaves - 1); i++) {
+                       float largest = -1000.0;
+                       
+                       if (m->control_pressed) { break; }
+                       
+                       int row, column;
+                       //find largest value in sims matrix by searching lower triangle
+                       for (int j = 1; j < sims.size(); j++) {
+                               for (int k = 0; k < j; k++) {
+                                       if (sims[j][k] > largest) {  largest = sims[j][k]; row = j; column = k;  }
+                               }
+                       }
+            
+                       //set non-leaf node info and update leaves to know their parents
+                       //non-leaf
+                       tree[numLeaves + i].setChildren(thisIndexes[row], thisIndexes[column]);
+                       
+                       //parents
+                       tree[thisIndexes[row]].setParent(numLeaves + i);
+                       tree[thisIndexes[column]].setParent(numLeaves + i);
+                       
+                       //blength = distance / 2;
+                       float blength = ((1.0 - largest) / 2);
+                       
+                       //branchlengths
+                       tree[thisIndexes[row]].setBranchLength(blength - tree[thisIndexes[row]].getLengthToLeaves());
+                       tree[thisIndexes[column]].setBranchLength(blength - tree[thisIndexes[column]].getLengthToLeaves());
+                       
+                       //set your length to leaves to your childs length plus branchlength
+                       tree[numLeaves + i].setLengthToLeaves(tree[thisIndexes[row]].getLengthToLeaves() + tree[thisIndexes[row]].getBranchLength());
+                       
+                       
+                       //update index 
+                       thisIndexes[row] = numLeaves+i;
+                       thisIndexes[column] = numLeaves+i;
+                       
+                       //remove highest value that caused the merge.
+                       sims[row][column] = -1000.0;
+                       sims[column][row] = -1000.0;
+                       
+                       //merge values in simsMatrix
+                       for (int n = 0; n < sims.size(); n++)   {
+                               //row becomes merge of 2 groups
+                               sims[row][n] = (sims[row][n] + sims[column][n]) / 2;
+                               sims[n][row] = sims[row][n];
+                               //delete column
+                               sims[column][n] = -1000.0;
+                               sims[n][column] = -1000.0;
+                       }
+               }
+               
+               //adjust tree to make sure root to tip length is .5
+               int root = findRoot();
+               tree[root].setBranchLength((0.5 - tree[root].getLengthToLeaves()));
+        
+    }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "Tree");
+               exit(1);
+       }
+}
 /*****************************************************************/
 Tree::~Tree() {}
-/*****************************************************************/
-void Tree::addNamesToCounts() {
+/*****************************************************************
+void Tree::addNamesToCounts(map<string, string> nameMap) {
        try {
                //ex. seq1      seq2,seq3,se4
                //              seq1 = pasture
@@ -68,22 +248,30 @@ void Tree::addNamesToCounts() {
 
                                
                //go through each leaf and update its pcounts and pgroups
+               
+               //float A = clock();
+
                for (int i = 0; i < numLeaves; i++) {
+
                        string name = tree[i].getName();
-                       
-                       map<string, string>::iterator itNames = globaldata->names.find(name);
-                       
-                       if (itNames == globaldata->names.end()) { mothurOut(name + " is not in your name file, please correct."); mothurOutEndLine(); exit(1);  }
+               
+                       map<string, string>::iterator itNames = nameMap.find(name);
+               
+                       if (itNames == nameMap.end()) { m->mothurOut(name + " is not in your name file, please correct."); m->mothurOutEndLine(); exit(1);  }
                        else {
                                vector<string> dupNames;
-                               splitAtComma(globaldata->names[name], dupNames);
+                               m->splitAtComma(nameMap[name], dupNames);
                                
                                map<string, int>::iterator itCounts;
                                int maxPars = 1;
+                               set<string> groupsAddedForThisNode;
                                for (int j = 0; j < dupNames.size(); j++) {
-                               
+                                       
+                                       string group = tmap->getGroup(dupNames[j]);
+                                       
                                        if (dupNames[j] != name) {//you already added yourself in the constructor
-                                               string group = globaldata->gTreemap->getGroup(dupNames[j]);
+                               
+                                               if (groupsAddedForThisNode.count(group) == 0)  {  groupNodeInfo[group].push_back(i);  groupsAddedForThisNode.insert(group);  } //if you have not already added this node for this group, then add it
                                                
                                                //update pcounts
                                                itCounts = tree[i].pcount.find(group);
@@ -97,7 +285,7 @@ void Tree::addNamesToCounts() {
                                                itCounts = tree[i].pGroups.find(group);
                                                if (itCounts == tree[i].pGroups.end()) { //new group, add it
                                                        tree[i].pGroups[group] = 1;
-                                               }else {
+                                               }else{
                                                        tree[i].pGroups[group]++;
                                                }
                                                
@@ -105,7 +293,7 @@ void Tree::addNamesToCounts() {
                                                if(tree[i].pGroups[group] > maxPars){
                                                        maxPars = tree[i].pGroups[group];
                                                }
-                                       }//end if
+                                       }else {  groupsAddedForThisNode.insert(group);  } //add it so you don't add it to groupNodeInfo again
                                }//end for
                                
                                if (maxPars > 1) { //then we have some more dominant groups
@@ -130,25 +318,28 @@ void Tree::addNamesToCounts() {
                                tree[i].setGroup(nodeGroups);
                                
                        }//end else
-               }//end for
-                               
+               }//end for              
+               
+               //float B = clock();
+               //cout << "addNamesToCounts\t" << (B - A) / CLOCKS_PER_SEC << endl;     
+
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "addNamesToCounts");
+               m->errorOut(e, "Tree", "addNamesToCounts");
                exit(1);
        }
-}
+}*/
 /*****************************************************************/
 int Tree::getIndex(string searchName) {
        try {
-               //Treemap knows name, group and index to speed up search
-               // getIndex function will return the vector index or -1 if seq is not found.
-               int index = globaldata->gTreemap->getIndex(searchName);
-               return index;
-               
+        map<string, int>::iterator itIndex = indexes.find(searchName);
+        if (itIndex != indexes.end()) {
+            return itIndex->second;
+        }
+               return -1;
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "getIndex");
+               m->errorOut(e, "Tree", "getIndex");
                exit(1);
        }
 }
@@ -156,29 +347,299 @@ int Tree::getIndex(string searchName) {
 
 void Tree::setIndex(string searchName, int index) {
        try {
-               //set index in treemap
-               globaldata->gTreemap->setIndex(searchName, index);
+               map<string, int>::iterator itIndex = indexes.find(searchName);
+        if (itIndex == indexes.end()) {
+            indexes[searchName] = index;
+        }
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "setIndex");
+               m->errorOut(e, "Tree", "setIndex");
                exit(1);
        }
 }
 /*****************************************************************/
-void Tree::assembleTree() {
+int Tree::assembleTree() {
+       try {           
+               //build the pGroups in non leaf nodes to be used in the parsimony calcs.
+               for (int i = numLeaves; i < numNodes; i++) {
+                       if (m->control_pressed) { return 1; }
+
+                       tree[i].pGroups = (mergeGroups(i));
+                       tree[i].pcount = (mergeGcounts(i));
+               }
+               
+               return 0;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "assembleTree");
+               exit(1);
+       }
+}
+/*****************************************************************/
+//assumes leaf node names are in groups and no names file - used by indicator command
+void Tree::getSubTree(Tree* Ctree, vector<string> Groups) {
        try {
-       
-               //if user has given a names file we want to include that info in the pgroups and pcount info.
-               if(globaldata->names.size() != 0) {  addNamesToCounts();  }
+        
+        //copy Tree since we are going to destroy it
+        Tree* copy = new Tree(ct);
+        copy->getCopy(Ctree);
+        copy->assembleTree();
+        
+               //we want to select some of the leaf nodes to create the output tree
+               //go through the input Tree starting at parents of leaves
+        //initialize groupNodeInfo
+        vector<string> namesOfGroups = ct->getNamesOfGroups();
+               for (int i = 0; i < namesOfGroups.size(); i++) {  groupNodeInfo[namesOfGroups[i]].resize(0);  }
                
-               //build the pGroups in non leaf nodes to be used in the parsimony calcs.
+               //initialize tree with correct number of nodes, name and group info.
+               for (int i = 0; i < numNodes; i++) {
+                       //initialize leaf nodes
+                       if (i <= (numLeaves-1)) {
+                               tree[i].setName(Groups[i]);
+                               
+                               //save group info
+                int maxPars = 1;
+                               vector<string> group;
+                vector<int> counts = ct->getGroupCounts(Groups[i]);
+                               for (int j = 0; j < namesOfGroups.size(); j++) {  
+                    if (counts[j] != 0) { //you have seqs from this group
+                        groupNodeInfo[namesOfGroups[j]].push_back(i);
+                        group.push_back(namesOfGroups[j]);
+                        tree[i].pGroups[namesOfGroups[j]] = counts[j];
+                        tree[i].pcount[namesOfGroups[j]] = counts[j];
+                        //keep highest group
+                                               if(counts[j] > maxPars){ maxPars = counts[j]; }
+                    }  
+                }
+                               tree[i].setGroup(group);
+                               setIndex(Groups[i], i);
+                
+                if (maxPars > 1) { //then we have some more dominant groups
+                                       //erase all the groups that are less than maxPars because you found a more dominant group.
+                                       for(it=tree[i].pGroups.begin();it!=tree[i].pGroups.end();){
+                                               if(it->second < maxPars){
+                                                       tree[i].pGroups.erase(it++);
+                                               }else { it++; }
+                                       }
+                                       //set one remaining groups to 1
+                                       for(it=tree[i].pGroups.begin();it!=tree[i].pGroups.end();it++){
+                                               tree[i].pGroups[it->first] = 1;
+                                       }
+                               }//end if
+                
+                //intialize non leaf nodes
+                       }else if (i > (numLeaves-1)) {
+                               tree[i].setName("");
+                               vector<string> tempGroups;
+                               tree[i].setGroup(tempGroups);
+                       }
+               }
+
+               set<int> removedLeaves;
+               for (int i = 0; i < copy->getNumLeaves(); i++) {
+                       
+                       if (removedLeaves.count(i) == 0) {
+                       
+                       //am I in the group
+                       int parent = copy->tree[i].getParent();
+                       
+                       if (parent != -1) {
+                               
+                               if (m->inUsersGroups(copy->tree[i].getName(), Groups)) {
+                                       //find my siblings name
+                                       int parentRC = copy->tree[parent].getRChild();
+                                       int parentLC = copy->tree[parent].getLChild();
+                                       
+                                       //if I am the right child, then my sib is the left child
+                                       int sibIndex = parentRC;
+                                       if (parentRC == i) { sibIndex = parentLC; }
+                                       
+                                       string sibsName = copy->tree[sibIndex].getName();
+                                       
+                                       //if yes, is my sibling
+                                       if ((m->inUsersGroups(sibsName, Groups)) || (sibsName == "")) {
+                                               //we both are okay no trimming required
+                                       }else{
+                                               //i am, my sib is not, so remove sib by setting my parent to my grandparent
+                                               int grandparent = copy->tree[parent].getParent();
+                                               int grandparentLC = copy->tree[grandparent].getLChild();
+                                               int grandparentRC = copy->tree[grandparent].getRChild();
+                                               
+                                               //whichever of my granparents children was my parent now equals me
+                                               if (grandparentLC == parent) { grandparentLC = i; }
+                                               else { grandparentRC = i; }
+                                               
+                                               copy->tree[i].setParent(grandparent);
+                                               copy->tree[i].setBranchLength((copy->tree[i].getBranchLength()+copy->tree[parent].getBranchLength()));
+                                               if (grandparent != -1) {
+                                                       copy->tree[grandparent].setChildren(grandparentLC, grandparentRC);
+                                               }
+                                               removedLeaves.insert(sibIndex);
+                                       }
+                               }else{
+                                       //find my siblings name
+                                       int parentRC = copy->tree[parent].getRChild();
+                                       int parentLC = copy->tree[parent].getLChild();
+                                       
+                                       //if I am the right child, then my sib is the left child
+                                       int sibIndex = parentRC;
+                                       if (parentRC == i) { sibIndex = parentLC; }
+                                       
+                                       string sibsName = copy->tree[sibIndex].getName();
+                                       
+                                       //if no is my sibling
+                                       if ((m->inUsersGroups(sibsName, Groups)) || (sibsName == "")) {
+                                               //i am not, but my sib is
+                                               int grandparent = copy->tree[parent].getParent();
+                                               int grandparentLC = copy->tree[grandparent].getLChild();
+                                               int grandparentRC = copy->tree[grandparent].getRChild();
+                                               
+                                               //whichever of my granparents children was my parent now equals my sib
+                                               if (grandparentLC == parent) { grandparentLC = sibIndex; }
+                                               else { grandparentRC = sibIndex; }
+                                               
+                                               copy->tree[sibIndex].setParent(grandparent);
+                                               copy->tree[sibIndex].setBranchLength((copy->tree[sibIndex].getBranchLength()+copy->tree[parent].getBranchLength()));
+                                               if (grandparent != -1) {
+                                                       copy->tree[grandparent].setChildren(grandparentLC, grandparentRC);
+                                               }
+                                               removedLeaves.insert(i);
+                                       }else{
+                                               //neither of us are, so we want to eliminate ourselves and our parent
+                                               //so set our parents sib to our great-grandparent
+                                               int parent = copy->tree[i].getParent();
+                                               int grandparent = copy->tree[parent].getParent();
+                                               int parentsSibIndex;
+                                               if (grandparent != -1) {
+                                                       int greatgrandparent = copy->tree[grandparent].getParent();
+                                                       int greatgrandparentLC, greatgrandparentRC;
+                                                       if (greatgrandparent != -1) {
+                                                               greatgrandparentLC = copy->tree[greatgrandparent].getLChild();
+                                                               greatgrandparentRC = copy->tree[greatgrandparent].getRChild();
+                                                       }
+                                                       
+                                                       int grandparentLC = copy->tree[grandparent].getLChild();
+                                                       int grandparentRC = copy->tree[grandparent].getRChild();
+                                                       
+                                                       parentsSibIndex = grandparentLC;
+                                                       if (grandparentLC == parent) { parentsSibIndex = grandparentRC; }
+
+                                                       //whichever of my greatgrandparents children was my grandparent
+                                                       if (greatgrandparentLC == grandparent) { greatgrandparentLC = parentsSibIndex; }
+                                                       else { greatgrandparentRC = parentsSibIndex; }
+                                                       
+                                                       copy->tree[parentsSibIndex].setParent(greatgrandparent);
+                                                       copy->tree[parentsSibIndex].setBranchLength((copy->tree[parentsSibIndex].getBranchLength()+copy->tree[grandparent].getBranchLength()));
+                                                       if (greatgrandparent != -1) {
+                                                               copy->tree[greatgrandparent].setChildren(greatgrandparentLC, greatgrandparentRC);
+                                                       }
+                                               }else{
+                                                       copy->tree[parent].setParent(-1);
+                                                       //cout << "issues with making subtree" << endl;
+                                               }
+                                               removedLeaves.insert(sibIndex);
+                                               removedLeaves.insert(i);
+                                       }
+                               }
+                       }
+                       }
+               }
+               
+               int root = 0;
+               for (int i = 0; i < copy->getNumNodes(); i++) {
+                       //you found the root
+                       if (copy->tree[i].getParent() == -1) { root = i; break; }
+               }
+        
+               int nextSpot = numLeaves;
+               populateNewTree(copy->tree, root, nextSpot);
+        
+        delete copy;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "getSubTree");
+               exit(1);
+       }
+}
+/*****************************************************************
+//assumes nameMap contains unique names as key or is empty. 
+//assumes numLeaves defined in tree constructor equals size of seqsToInclude and seqsToInclude only contains unique seqs.
+int Tree::getSubTree(Tree* copy, vector<string> seqsToInclude, map<string, string> nameMap) {
+       try {
+        
+        if (numLeaves != seqsToInclude.size()) { m->mothurOut("[ERROR]: numLeaves does not equal numUniques, cannot create subtree.\n"); m->control_pressed = true; return 0; }
+        
+        getSubTree(copy, seqsToInclude);
+        if (nameMap.size() != 0) {  addNamesToCounts(nameMap);  }
+        
+        //build the pGroups in non leaf nodes to be used in the parsimony calcs.
+               for (int i = numLeaves; i < numNodes; i++) {
+                       if (m->control_pressed) { return 1; }
+            
+                       tree[i].pGroups = (mergeGroups(i));
+                       tree[i].pcount = (mergeGcounts(i));
+               }
+        
+        return 0;
+    }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "getSubTree");
+               exit(1);
+       }
+}
+/*****************************************************************/
+int Tree::populateNewTree(vector<Node>& oldtree, int node, int& index) {
+       try {
+               
+               if (oldtree[node].getLChild() != -1) {
+                       int rc = populateNewTree(oldtree, oldtree[node].getLChild(), index);
+                       int lc = populateNewTree(oldtree, oldtree[node].getRChild(), index);
+
+                       tree[index].setChildren(lc, rc);
+                       tree[rc].setParent(index);
+                       tree[lc].setParent(index);
+                       
+                       tree[index].setBranchLength(oldtree[node].getBranchLength());
+                       tree[rc].setBranchLength(oldtree[oldtree[node].getLChild()].getBranchLength());
+                       tree[lc].setBranchLength(oldtree[oldtree[node].getRChild()].getBranchLength());
+                       
+                       return (index++);
+               }else { //you are a leaf
+                       int indexInNewTree = getIndex(oldtree[node].getName());
+                       return indexInNewTree;
+               }
+       }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "populateNewTree");
+               exit(1);
+       }
+}
+/*****************************************************************/
+void Tree::getCopy(Tree* copy, bool subsample) {
+       try {
+        
+               //for each node in the tree copy its info
+               for (int i = 0; i < numNodes; i++) {
+                       //copy branch length
+                       tree[i].setBranchLength(copy->tree[i].getBranchLength());
+            
+                       //copy parent
+                       tree[i].setParent(copy->tree[i].getParent());
+            
+                       //copy children
+                       tree[i].setChildren(copy->tree[i].getLChild(), copy->tree[i].getRChild());
+        }
+        
+        //build the pGroups in non leaf nodes to be used in the parsimony calcs.
                for (int i = numLeaves; i < numNodes; i++) {
+                       if (m->control_pressed) { break; }
+            
                        tree[i].pGroups = (mergeGroups(i));
                        tree[i].pcount = (mergeGcounts(i));
                }
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "assembleTree");
+               m->errorOut(e, "Tree", "getCopy");
                exit(1);
        }
 }
@@ -204,8 +665,8 @@ void Tree::getCopy(Tree* copy) {
                        tree[i].setChildren(copy->tree[i].getLChild(), copy->tree[i].getRChild());
                
                        //copy index in node and tmap
+            setIndex(copy->tree[i].getName(), getIndex(copy->tree[i].getName()));
                        tree[i].setIndex(copy->tree[i].getIndex());
-                       setIndex(copy->tree[i].getName(), getIndex(copy->tree[i].getName()));
                        
                        //copy pGroups
                        tree[i].pGroups = copy->tree[i].pGroups;
@@ -214,9 +675,11 @@ void Tree::getCopy(Tree* copy) {
                        tree[i].pcount = copy->tree[i].pcount;
                }
                
+               groupNodeInfo = copy->groupNodeInfo;
+               
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "getCopy");
+               m->errorOut(e, "Tree", "getCopy");
                exit(1);
        }
 }
@@ -269,7 +732,7 @@ map<string, int> Tree::mergeGroups(int i) {
                return parsimony;
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "mergeGroups");
+               m->errorOut(e, "Tree", "mergeGroups");
                exit(1);
        }
 }
@@ -287,14 +750,14 @@ map<string, int> Tree::mergeUserGroups(int i, vector<string> g) {
                
                //loop through nodes groups removing the ones the user doesn't want
                for(it=tree[lc].pGroups.begin();it!=tree[lc].pGroups.end();){
-                               if (inUsersGroups(it->first, g) != true) {
+                               if (m->inUsersGroups(it->first, g) != true) {
                                        tree[lc].pGroups.erase(it++);
                                }else { it++; }
                }
 
                //loop through nodes groups removing the ones the user doesn't want
                for(it=tree[rc].pGroups.begin();it!=tree[rc].pGroups.end();){
-                               if (inUsersGroups(it->first, g) != true) {
+                               if (m->inUsersGroups(it->first, g) != true) {
                                        tree[rc].pGroups.erase(it++);
                                }else { it++; }
                }
@@ -335,7 +798,7 @@ map<string, int> Tree::mergeUserGroups(int i, vector<string> g) {
                return parsimony;
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "mergeUserGroups");
+               m->errorOut(e, "Tree", "mergeUserGroups");
                exit(1);
        }
 }
@@ -358,14 +821,18 @@ map<string,int> Tree::mergeGcounts(int position) {
                return sum;
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "mergeGcounts");
+               m->errorOut(e, "Tree", "mergeGcounts");
                exit(1);
        }
 }
 /**************************************************************************************************/
-
 void Tree::randomLabels(vector<string> g) {
        try {
+       
+               //initialize groupNodeInfo
+               for (int i = 0; i < (ct->getNamesOfGroups()).size(); i++) {
+                       groupNodeInfo[(ct->getNamesOfGroups())[i]].resize(0);
+               }
                
                for(int i = 0; i < numLeaves; i++){
                        int z;
@@ -376,8 +843,8 @@ void Tree::randomLabels(vector<string> g) {
                        //if either of the leaf nodes you are about to switch are not in the users groups then you don't want to switch them.
                        bool treez, treei;
                
-                       treez = inUsersGroups(tree[z].getGroup(), g);
-                       treei = inUsersGroups(tree[i].getGroup(), g);
+                       treez = m->inUsersGroups(tree[z].getGroup(), g);
+                       treei = m->inUsersGroups(tree[i].getGroup(), g);
                        
                        if ((treez == true) && (treei == true)) {
                                //switches node i and node z's info.
@@ -397,40 +864,13 @@ void Tree::randomLabels(vector<string> g) {
                                tree[z].pcount = (tree[i].pcount);
                                tree[i].pcount = (gcount_hold);
                        }
+                       
+                       for (int k = 0; k < (tree[i].getGroup()).size(); k++) {  groupNodeInfo[(tree[i].getGroup())[k]].push_back(i); }
+                       for (int k = 0; k < (tree[z].getGroup()).size(); k++) {  groupNodeInfo[(tree[z].getGroup())[k]].push_back(z); }
                }
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "randomLabels");
-               exit(1);
-       }
-}
-/**************************************************************************************************
-
-void Tree::randomLabels(string groupA, string groupB) {
-       try {
-               int numSeqsA = globaldata->gTreemap->seqsPerGroup[groupA];
-               int numSeqsB = globaldata->gTreemap->seqsPerGroup[groupB];
-
-               vector<string> randomGroups(numSeqsA+numSeqsB, groupA);
-               for(int i=numSeqsA;i<randomGroups.size();i++){
-                       randomGroups[i] = groupB;
-               }
-               random_shuffle(randomGroups.begin(), randomGroups.end());
-                               
-               int randomCounter = 0;                          
-               for(int i=0;i<numLeaves;i++){
-                       if(tree[i].getGroup() == groupA || tree[i].getGroup() == groupB){
-                               tree[i].setGroup(randomGroups[randomCounter]);
-                               tree[i].pcount.clear();
-                               tree[i].pcount[randomGroups[randomCounter]] = 1;
-                               tree[i].pGroups.clear();
-                               tree[i].pGroups[randomGroups[randomCounter]] = 1;
-                               randomCounter++;
-                       }
-               }
-       }               
-       catch(exception& e) {
-               errorOut(e, "Tree", "randomLabels");
+               m->errorOut(e, "Tree", "randomLabels");
                exit(1);
        }
 }
@@ -446,7 +886,7 @@ void Tree::randomBlengths()  {
                }
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "randomBlengths");
+               m->errorOut(e, "Tree", "randomBlengths");
                exit(1);
        }
 }
@@ -457,7 +897,6 @@ void Tree::assembleRandomUnifracTree(vector<string> g) {
 }
 /*************************************************************************************************/
 void Tree::assembleRandomUnifracTree(string groupA, string groupB) {
-
        vector<string> temp; temp.push_back(groupA); temp.push_back(groupB);
        randomLabels(temp);
        assembleTree();
@@ -503,7 +942,7 @@ void Tree::randomTopology() {
                }
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "randomTopology");
+               m->errorOut(e, "Tree", "randomTopology");
                exit(1);
        }
 }
@@ -515,32 +954,43 @@ void Tree::print(ostream& out) {
                out << ";" << endl;
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "print");
+               m->errorOut(e, "Tree", "print");
                exit(1);
        }
 }
 /*****************************************************************/
-void Tree::printForBoot(ostream& out) {
+void Tree::print(ostream& out, map<string, string> nameMap) {
        try {
                int root = findRoot();
-               printBranch(root, out, "boot");
+               printBranch(root, out, nameMap);
                out << ";" << endl;
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "printForBoot");
+               m->errorOut(e, "Tree", "print");
+               exit(1);
+       }
+}
+/*****************************************************************/
+void Tree::print(ostream& out, string mode) {
+       try {
+               int root = findRoot();
+               printBranch(root, out, mode);
+               out << ";" << endl;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "print");
                exit(1);
        }
 }
-
 /*****************************************************************/
 // This prints out the tree in Newick form.
 void Tree::createNewickFile(string f) {
        try {
                int root = findRoot();
-               //filename = getRootName(globaldata->getTreeFile()) + "newick";
+       
                filename = f;
 
-               openOutputFile(filename, out);
+               m->openOutputFile(filename, out);
                
                printBranch(root, out, "branch");
                
@@ -549,7 +999,7 @@ void Tree::createNewickFile(string f) {
                out.close();
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "createNewickFile");
+               m->errorOut(e, "Tree", "createNewickFile");
                exit(1);
        }
 }
@@ -568,16 +1018,87 @@ int Tree::findRoot() {
                return -1;
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "findRoot");
+               m->errorOut(e, "Tree", "findRoot");
                exit(1);
        }
 }
+/*****************************************************************/
+void Tree::printBranch(int node, ostream& out, map<string, string> names) {
+try {
 
+// you are not a leaf
+               if (tree[node].getLChild() != -1) {
+                       out << "(";
+                       printBranch(tree[node].getLChild(), out, names);
+                       out << ",";
+                       printBranch(tree[node].getRChild(), out, names);
+                       out << ")";
+                       
+            //if there is a branch length then print it
+            if (tree[node].getBranchLength() != -1) {
+                out << ":" << tree[node].getBranchLength();
+            }
+                       
+               }else { //you are a leaf
+            map<string, string>::iterator itNames = names.find(tree[node].getName());
+            
+            string outputString = "";
+            if (itNames != names.end()) { 
+                
+                vector<string> dupNames;
+                m->splitAtComma((itNames->second), dupNames);
+                
+                if (dupNames.size() == 1) {
+                    outputString += tree[node].getName();
+                    if (tree[node].getBranchLength() != -1) {
+                        outputString += ":" + toString(tree[node].getBranchLength());
+                    }
+                }else {
+                    outputString += "(";
+                    
+                    for (int u = 0; u < dupNames.size()-1; u++) {
+                        outputString += dupNames[u];
+                        
+                        if (tree[node].getBranchLength() != -1) {
+                            outputString += ":" + toString(0.0);
+                        }
+                        outputString += ",";
+                    }
+                    
+                    outputString += dupNames[dupNames.size()-1];
+                    if (tree[node].getBranchLength() != -1) {
+                        outputString += ":" + toString(0.0);
+                    }
+                    
+                    outputString += ")";
+                    if (tree[node].getBranchLength() != -1) {
+                        outputString += ":" + toString(tree[node].getBranchLength());
+                    }
+                }
+            }else { 
+                outputString = tree[node].getName();
+                //if there is a branch length then print it
+                if (tree[node].getBranchLength() != -1) {
+                    outputString += ":" + toString(tree[node].getBranchLength());
+                }
+                
+                m->mothurOut("[ERROR]: " + tree[node].getName() + " is not in your namefile, please correct."); m->mothurOutEndLine(); 
+            }
+                
+            out << outputString;
+               }
+               
+       }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "printBranch");
+               exit(1);
+       }
+}
 /*****************************************************************/
 void Tree::printBranch(int node, ostream& out, string mode) {
-       try {
-               
-               // you are not a leaf
+    try {
+        
+        // you are not a leaf
                if (tree[node].getLChild() != -1) {
                        out << "(";
                        printBranch(tree[node].getLChild(), out, mode);
@@ -594,31 +1115,111 @@ void Tree::printBranch(int node, ostream& out, string mode) {
                                if (tree[node].getLabel() != -1) {
                                        out << tree[node].getLabel();
                                }
+                       }else if (mode == "both") {
+                               if (tree[node].getLabel() != -1) {
+                                       out << tree[node].getLabel();
+                               }
+                               //if there is a branch length then print it
+                               if (tree[node].getBranchLength() != -1) {
+                                       out << ":" << tree[node].getBranchLength();
+                               }
                        }
                }else { //you are a leaf
-                       string leafGroup = globaldata->gTreemap->getGroup(tree[node].getName());
+                       vector<string> leafGroup = ct->getGroups(tree[node].getName());
                        
-                       out << leafGroup; 
                        if (mode == "branch") {
+                               out << leafGroup[0]; 
                                //if there is a branch length then print it
                                if (tree[node].getBranchLength() != -1) {
                                        out << ":" << tree[node].getBranchLength();
                                }
                        }else if (mode == "boot") {
+                               out << leafGroup[0]; 
                                //if there is a label then print it
                                if (tree[node].getLabel() != -1) {
                                        out << tree[node].getLabel();
                                }
+                       }else if (mode == "both") {
+                               out << tree[node].getName();
+                               if (tree[node].getLabel() != -1) {
+                                       out << tree[node].getLabel();
+                               }
+                               //if there is a branch length then print it
+                               if (tree[node].getBranchLength() != -1) {
+                                       out << ":" << tree[node].getBranchLength();
+                               }
                        }
                }
                
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "printBranch");
+               m->errorOut(e, "Tree", "printBranch");
+               exit(1);
+       }
+}
+/*****************************************************************/
+void Tree::printBranch(int node, ostream& out, string mode, vector<Node>& theseNodes) {
+       try {
+               
+               // you are not a leaf
+               if (theseNodes[node].getLChild() != -1) {
+                       out << "(";
+                       printBranch(theseNodes[node].getLChild(), out, mode);
+                       out << ",";
+                       printBranch(theseNodes[node].getRChild(), out, mode);
+                       out << ")";
+                       if (mode == "branch") {
+                               //if there is a branch length then print it
+                               if (theseNodes[node].getBranchLength() != -1) {
+                                       out << ":" << theseNodes[node].getBranchLength();
+                               }
+                       }else if (mode == "boot") {
+                               //if there is a label then print it
+                               if (theseNodes[node].getLabel() != -1) {
+                                       out << theseNodes[node].getLabel();
+                               }
+                       }else if (mode == "both") {
+                               if (theseNodes[node].getLabel() != -1) {
+                                       out << theseNodes[node].getLabel();
+                               }
+                               //if there is a branch length then print it
+                               if (theseNodes[node].getBranchLength() != -1) {
+                                       out << ":" << theseNodes[node].getBranchLength();
+                               }
+                       }
+               }else { //you are a leaf
+                       vector<string> leafGroup = ct->getGroups(theseNodes[node].getName());
+                       
+                       if (mode == "branch") {
+                               out << leafGroup[0]; 
+                               //if there is a branch length then print it
+                               if (theseNodes[node].getBranchLength() != -1) {
+                                       out << ":" << theseNodes[node].getBranchLength();
+                               }
+                       }else if (mode == "boot") {
+                               out << leafGroup[0]; 
+                               //if there is a label then print it
+                               if (theseNodes[node].getLabel() != -1) {
+                                       out << theseNodes[node].getLabel();
+                               }
+                       }else if (mode == "both") {
+                               out << theseNodes[node].getName();
+                               if (theseNodes[node].getLabel() != -1) {
+                                       out << theseNodes[node].getLabel();
+                               }
+                               //if there is a branch length then print it
+                               if (theseNodes[node].getBranchLength() != -1) {
+                                       out << ":" << theseNodes[node].getBranchLength();
+                               }
+                       }
+               }
+               
+       }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "printBranch");
                exit(1);
        }
 }
-
 /*****************************************************************/
 
 void Tree::printTree() {
@@ -632,21 +1233,23 @@ void Tree::printTree() {
 
 /*****************************************************************/
 //this code is a mess and should be rethought...-slw
-void Tree::parseTreeFile() {
+int Tree::parseTreeFile() {
        
        //only takes names from the first tree and assumes that all trees use the same names.
        try {
-               string filename = globaldata->getTreeFile();
+               string filename = m->getTreeFile();
                ifstream filehandle;
-               openInputFile(filename, filehandle);
+               m->openInputFile(filename, filehandle);
                int c, comment;
                comment = 0;
                int done = 1;
                
                //ifyou are not a nexus file 
                if((c = filehandle.peek()) != '#') {  
-                       while((c = filehandle.peek()) != ';') { 
+                       while((c = filehandle.peek()) != ';') {
+                if (m->control_pressed) {  filehandle.close(); return 0; }
                                while ((c = filehandle.peek()) != ';') {
+                    if (m->control_pressed) {  filehandle.close(); return 0; }
                                        // get past comments
                                        if(c == '[') {
                                                comment = 1;
@@ -666,7 +1269,8 @@ void Tree::parseTreeFile() {
                        string holder = "";
                                        
                        // get past comments
-                       while(holder != "translate" && holder != "Translate"){  
+                       while(holder != "translate" && holder != "Translate"){
+                if (m->control_pressed) {  filehandle.close(); return 0; }
                                if(holder == "[" || holder == "[!"){
                                        comment = 1;
                                }
@@ -695,22 +1299,27 @@ void Tree::parseTreeFile() {
 
                                string number, name, h;
                                h = ""; // so it enters the loop the first time
-                               while((h != ";") && (number != ";")) { 
+                               while((h != ";") && (number != ";")) {
+                    if (m->control_pressed) {  filehandle.close(); return 0; }
                                        filehandle >> number;
                                        filehandle >> name;
        
                                        //c = , until done with translation then c = ;
                                        h = name.substr(name.length()-1, name.length()); 
                                        name.erase(name.end()-1);  //erase the comma
-                                       globaldata->Treenames.push_back(number);
+                                       m->Treenames.push_back(number);
                                }
-                               if(number == ";") { globaldata->Treenames.pop_back(); }  //in case ';' from translation is on next line instead of next to last name
+                               if(number == ";") { m->Treenames.pop_back(); }  //in case ';' from translation is on next line instead of next to last name
                        }
                }
                filehandle.close();
+               return 0;
+               //for (int i = 0; i < globaldata->Treenames.size(); i++) {
+//cout << globaldata->Treenames[i] << endl; }
+//cout << globaldata->Treenames.size() << endl;
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "parseTreeFile");
+               m->errorOut(e, "Tree", "parseTreeFile");
                exit(1);
        }
 }
@@ -722,7 +1331,8 @@ int Tree::readTreeString(ifstream& filehandle)     {
                int c;
                string name;  //, k
                
-               while((c = filehandle.peek()) != ';') { 
+               while((c = filehandle.peek()) != ';') {
+            if (m->control_pressed) {  return 0; }
 //k = c;
 //cout << " at beginning of while " <<  k << endl;                     
                        if(c == ')')  {    
@@ -739,7 +1349,7 @@ int Tree::readTreeString(ifstream& filehandle)     {
                                c = filehandle.get();
                        //k = c;
 //cout << k << endl;
-                               while ((c != '(') && (c != ')') && (c != ',') && (c != ':') && (c != '\n') && (c != 32) && (c != '\t')) {                       
+                               while ((c != '(') && (c != ')') && (c != ',') && (c != ':')  && (c != '\n') && (c != 32) && (c != '\t')) {                      
                                        name += c;
                                        c = filehandle.get();
                        //k = c;
@@ -747,7 +1357,9 @@ int Tree::readTreeString(ifstream& filehandle)     {
                                }
                                
 //cout << "name = " << name << endl;
-                               globaldata->Treenames.push_back(name);
+                if (name != "\r" ) {
+                    m->Treenames.push_back(name);   } //cout << m->Treenames.size() << '\t' << name << endl;
+                
                                filehandle.putback(c);
 //k = c;
 //cout << " after putback" <<  k << endl;
@@ -774,7 +1386,7 @@ int Tree::readTreeString(ifstream& filehandle)     {
                return 0;
        }
        catch(exception& e) {
-               errorOut(e, "Tree", "readTreeString");
+               m->errorOut(e, "Tree", "readTreeString");
                exit(1);
        }
 }