]> git.donarmstrong.com Git - mothur.git/blobdiff - tree.cpp
added design parameter to the indicator command
[mothur.git] / tree.cpp
index 3b3b6840a6fd1a427258e7b84d0afe3bf181bd7e..08ee850f75a485e6f10830e1c244cc26bd4107f5 100644 (file)
--- a/tree.cpp
+++ b/tree.cpp
 
 #include "tree.h"
 
-
+/*****************************************************************/
+Tree::Tree(int num) {
+       try {
+               globaldata = GlobalData::getInstance();
+               m = MothurOut::getInstance();
+               
+               numLeaves = num;  
+               numNodes = 2*numLeaves - 1;
+               
+               tree.resize(numNodes);
+       }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "Tree - numNodes");
+               exit(1);
+       }
+}
+/*****************************************************************/
+Tree::Tree(string g) {
+       try {
+               globaldata = GlobalData::getInstance();
+               m = MothurOut::getInstance();
+               
+               parseTreeFile();  globaldata->runParse = false;  
+       }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "Tree - just parse");
+               exit(1);
+       }
+}
 /*****************************************************************/
 Tree::Tree() {
        try {
-       
                globaldata = GlobalData::getInstance();
-               numLeaves = globaldata->gTreemap->getNumSeqs();
+               m = MothurOut::getInstance();
+               
+               if (globaldata->runParse == true) {  parseTreeFile();  globaldata->runParse = false;  }
+//for(int i = 0; i <   globaldata->Treenames.size(); i++) { cout << i << '\t' << globaldata->Treenames[i] << endl;  }  
+               numLeaves = globaldata->Treenames.size();
                numNodes = 2*numLeaves - 1;
                
                tree.resize(numNodes);
+               
+               //initialize groupNodeInfo
+               for (int i = 0; i < globaldata->gTreemap->namesOfGroups.size(); i++) {
+                       groupNodeInfo[globaldata->gTreemap->namesOfGroups[i]].resize(0);
+               }
 
                //initialize tree with correct number of nodes, name and group info.
                for (int i = 0; i < numNodes; i++) {
                        //initialize leaf nodes
                        if (i <= (numLeaves-1)) {
-                               tree[i].setName(globaldata->gTreemap->namesOfSeqs[i]);
-                               tree[i].setGroup(globaldata->gTreemap->getGroup(globaldata->gTreemap->namesOfSeqs[i]));
-                               //the node knows its index
-                               tree[i].setIndex(i);
+                               tree[i].setName(globaldata->Treenames[i]);
+                               
+                               //save group info
+                               string group = globaldata->gTreemap->getGroup(globaldata->Treenames[i]);
+                               vector<string> tempGroups; tempGroups.push_back(group);
+                               tree[i].setGroup(tempGroups);
+                               groupNodeInfo[group].push_back(i); 
+                               
                                //set pcount and pGroup for groupname to 1.
-                               tree[i].pcount[globaldata->gTreemap->getGroup(globaldata->gTreemap->namesOfSeqs[i])] = 1;
-                               tree[i].pGroups[globaldata->gTreemap->getGroup(globaldata->gTreemap->namesOfSeqs[i])] = 1;
+                               tree[i].pcount[group] = 1;
+                               tree[i].pGroups[group] = 1;
+                               
                                //Treemap knows name, group and index to speed up search
-                               globaldata->gTreemap->setIndex(globaldata->gTreemap->namesOfSeqs[i], i);
+                               globaldata->gTreemap->setIndex(globaldata->Treenames[i], i);
        
                        //intialize non leaf nodes
                        }else if (i > (numLeaves-1)) {
                                tree[i].setName("");
-                               tree[i].setGroup("");
-                               //the node knows its index
-                               tree[i].setIndex(i);
+                               vector<string> tempGroups;
+                               tree[i].setGroup(tempGroups);
                        }
                }
        }
        catch(exception& e) {
-               cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function Tree. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+               m->errorOut(e, "Tree", "Tree");
                exit(1);
        }
-       catch(...) {
-               cout << "An unknown error has occurred in the Tree class function Tree. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
-               exit(1);
-       }               
 }
 
 /*****************************************************************/
+Tree::~Tree() {}
+/*****************************************************************/
+void Tree::addNamesToCounts() {
+       try {
+               //ex. seq1      seq2,seq3,se4
+               //              seq1 = pasture
+               //              seq2 = forest
+               //              seq4 = pasture
+               //              seq3 = ocean
+               
+               //before this function seq1.pcount = pasture -> 1
+               //after                            seq1.pcount = pasture -> 2, forest -> 1, ocean -> 1
+               
+               //before this function seq1.pgroups = pasture -> 1
+               //after                            seq1.pgroups = pasture -> 1 since that is the dominant group
 
+                               
+               //go through each leaf and update its pcounts and pgroups
+               
+               //float A = clock();
+
+               for (int i = 0; i < numLeaves; i++) {
+
+                       string name = tree[i].getName();
+               
+                       map<string, string>::iterator itNames = globaldata->names.find(name);
+               
+                       if (itNames == globaldata->names.end()) { m->mothurOut(name + " is not in your name file, please correct."); m->mothurOutEndLine(); exit(1);  }
+                       else {
+                               vector<string> dupNames;
+                               m->splitAtComma(globaldata->names[name], dupNames);
+                               
+                               map<string, int>::iterator itCounts;
+                               int maxPars = 1;
+                               set<string> groupsAddedForThisNode;
+                               for (int j = 0; j < dupNames.size(); j++) {
+                                       
+                                       string group = globaldata->gTreemap->getGroup(dupNames[j]);
+                                       
+                                       if (dupNames[j] != name) {//you already added yourself in the constructor
+                               
+                                               if (groupsAddedForThisNode.count(group) == 0)  {  groupNodeInfo[group].push_back(i);  groupsAddedForThisNode.insert(group);  } //if you have not already added this node for this group, then add it
+                                               
+                                               //update pcounts
+                                               itCounts = tree[i].pcount.find(group);
+                                               if (itCounts == tree[i].pcount.end()) { //new group, add it
+                                                       tree[i].pcount[group] = 1;
+                                               }else {
+                                                       tree[i].pcount[group]++;
+                                               }
+                                                       
+                                               //update pgroups
+                                               itCounts = tree[i].pGroups.find(group);
+                                               if (itCounts == tree[i].pGroups.end()) { //new group, add it
+                                                       tree[i].pGroups[group] = 1;
+                                               }else{
+                                                       tree[i].pGroups[group]++;
+                                               }
+                                               
+                                               //keep highest group
+                                               if(tree[i].pGroups[group] > maxPars){
+                                                       maxPars = tree[i].pGroups[group];
+                                               }
+                                       }else {  groupsAddedForThisNode.insert(group);  } //add it so you don't add it to groupNodeInfo again
+                               }//end for
+                               
+                               if (maxPars > 1) { //then we have some more dominant groups
+                                       //erase all the groups that are less than maxPars because you found a more dominant group.
+                                       for(it=tree[i].pGroups.begin();it!=tree[i].pGroups.end();){
+                                               if(it->second < maxPars){
+                                                       tree[i].pGroups.erase(it++);
+                                               }else { it++; }
+                                       }
+                                       //set one remaining groups to 1
+                                       for(it=tree[i].pGroups.begin();it!=tree[i].pGroups.end();it++){
+                                               tree[i].pGroups[it->first] = 1;
+                                       }
+                               }//end if
+                               
+                               //update groups to reflect all the groups this node represents
+                               vector<string> nodeGroups;
+                               map<string, int>::iterator itGroups;
+                               for (itGroups = tree[i].pcount.begin(); itGroups != tree[i].pcount.end(); itGroups++) {
+                                       nodeGroups.push_back(itGroups->first);
+                               }
+                               tree[i].setGroup(nodeGroups);
+                               
+                       }//end else
+               }//end for              
+               
+               //float B = clock();
+               //cout << "addNamesToCounts\t" << (B - A) / CLOCKS_PER_SEC << endl;     
+
+       }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "addNamesToCounts");
+               exit(1);
+       }
+}
+/*****************************************************************/
 int Tree::getIndex(string searchName) {
        try {
                //Treemap knows name, group and index to speed up search
@@ -64,13 +199,9 @@ int Tree::getIndex(string searchName) {
                
        }
        catch(exception& e) {
-               cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function getIndex. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+               m->errorOut(e, "Tree", "getIndex");
                exit(1);
        }
-       catch(...) {
-               cout << "An unknown error has occurred in the Tree class function getIndex. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
-               exit(1);
-       }               
 }
 /*****************************************************************/
 
@@ -80,31 +211,229 @@ void Tree::setIndex(string searchName, int index) {
                globaldata->gTreemap->setIndex(searchName, index);
        }
        catch(exception& e) {
-               cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function setIndex. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+               m->errorOut(e, "Tree", "setIndex");
                exit(1);
        }
-       catch(...) {
-               cout << "An unknown error has occurred in the Tree class function setIndex. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+}
+/*****************************************************************/
+int Tree::assembleTree() {
+       try {
+               //float A = clock();
+
+               //if user has given a names file we want to include that info in the pgroups and pcount info.
+               if(globaldata->names.size() != 0) {  addNamesToCounts();  }
+               
+               //build the pGroups in non leaf nodes to be used in the parsimony calcs.
+               for (int i = numLeaves; i < numNodes; i++) {
+                       if (m->control_pressed) { return 1; }
+
+                       tree[i].pGroups = (mergeGroups(i));
+                       tree[i].pcount = (mergeGcounts(i));
+               }
+               //float B = clock();
+               //cout << "assembleTree\t" << (B-A) / CLOCKS_PER_SEC << endl;
+               return 0;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "assembleTree");
                exit(1);
-       }               
+       }
 }
 /*****************************************************************/
-void Tree::assembleTree() {
+int Tree::assembleTree(string n) {
        try {
+               
                //build the pGroups in non leaf nodes to be used in the parsimony calcs.
                for (int i = numLeaves; i < numNodes; i++) {
+                       if (m->control_pressed) { return 1; }
+
                        tree[i].pGroups = (mergeGroups(i));
                        tree[i].pcount = (mergeGcounts(i));
                }
+               //float B = clock();
+               //cout << "assembleTree\t" << (B-A) / CLOCKS_PER_SEC << endl;
+               return 0;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "assembleTree");
+               exit(1);
+       }
+}
+/*****************************************************************/
+void Tree::getSubTree(Tree* copy, vector<string> Groups) {
+       try {
+                       
+               //we want to select some of the leaf nodes to create the output tree
+               //go through the input Tree starting at parents of leaves
+               for (int i = 0; i < numNodes; i++) {
+                       
+                       //initialize leaf nodes
+                       if (i <= (numLeaves-1)) {
+                               tree[i].setName(Groups[i]);
+                               
+                               //save group info
+                               string group = globaldata->gTreemap->getGroup(Groups[i]);
+                               vector<string> tempGroups; tempGroups.push_back(group);
+                               tree[i].setGroup(tempGroups);
+                               groupNodeInfo[group].push_back(i); 
+                               
+                               //set pcount and pGroup for groupname to 1.
+                               tree[i].pcount[group] = 1;
+                               tree[i].pGroups[group] = 1;
+                               
+                               //Treemap knows name, group and index to speed up search
+                               globaldata->gTreemap->setIndex(Groups[i], i);
+                               
+                               //intialize non leaf nodes
+                       }else if (i > (numLeaves-1)) {
+                               tree[i].setName("");
+                               vector<string> tempGroups;
+                               tree[i].setGroup(tempGroups);
+                       }
+               }
+               
+               set<int> removedLeaves;
+               for (int i = 0; i < copy->getNumLeaves(); i++) {
+                       
+                       if (removedLeaves.count(i) == 0) {
+                       
+                       //am I in the group
+                       int parent = copy->tree[i].getParent();
+                       
+                       if (parent != -1) {
+                               
+                               if (m->inUsersGroups(copy->tree[i].getName(), Groups)) {
+                                       //find my siblings name
+                                       int parentRC = copy->tree[parent].getRChild();
+                                       int parentLC = copy->tree[parent].getLChild();
+                                       
+                                       //if I am the right child, then my sib is the left child
+                                       int sibIndex = parentRC;
+                                       if (parentRC == i) { sibIndex = parentLC; }
+                                       
+                                       string sibsName = copy->tree[sibIndex].getName();
+                                       
+                                       //if yes, is my sibling
+                                       if ((m->inUsersGroups(sibsName, Groups)) || (sibsName == "")) {
+                                               //we both are okay no trimming required
+                                       }else{
+                                               //i am, my sib is not, so remove sib by setting my parent to my grandparent
+                                               int grandparent = copy->tree[parent].getParent();
+                                               int grandparentLC = copy->tree[grandparent].getLChild();
+                                               int grandparentRC = copy->tree[grandparent].getRChild();
+                                               
+                                               //whichever of my granparents children was my parent now equals me
+                                               if (grandparentLC == parent) { grandparentLC = i; }
+                                               else { grandparentRC = i; }
+                                               
+                                               copy->tree[i].setParent(grandparent);
+                                               copy->tree[i].setBranchLength((copy->tree[i].getBranchLength()+copy->tree[parent].getBranchLength()));
+                                               copy->tree[grandparent].setChildren(grandparentLC, grandparentRC);
+                                               removedLeaves.insert(sibIndex);
+                                       }
+                               }else{
+                                       //find my siblings name
+                                       int parentRC = copy->tree[parent].getRChild();
+                                       int parentLC = copy->tree[parent].getLChild();
+                                       
+                                       //if I am the right child, then my sib is the left child
+                                       int sibIndex = parentRC;
+                                       if (parentRC == i) { sibIndex = parentLC; }
+                                       
+                                       string sibsName = copy->tree[sibIndex].getName();
+                                       
+                                       //if no is my sibling
+                                       if ((m->inUsersGroups(sibsName, Groups)) || (sibsName == "")) {
+                                               //i am not, but my sib is
+                                               int grandparent = copy->tree[parent].getParent();
+                                               int grandparentLC = copy->tree[grandparent].getLChild();
+                                               int grandparentRC = copy->tree[grandparent].getRChild();
+                                               
+                                               //whichever of my granparents children was my parent now equals my sib
+                                               if (grandparentLC == parent) { grandparentLC = sibIndex; }
+                                               else { grandparentRC = sibIndex; }
+                                               
+                                               copy->tree[sibIndex].setParent(grandparent);
+                                               copy->tree[sibIndex].setBranchLength((copy->tree[sibIndex].getBranchLength()+copy->tree[parent].getBranchLength()));
+                                               copy->tree[grandparent].setChildren(grandparentLC, grandparentRC);
+                                               removedLeaves.insert(i);
+                                       }else{
+                                               //neither of us are, so we want to eliminate ourselves and our parent
+                                               //so set our parents sib to our great-grandparent
+                                               int parent = copy->tree[i].getParent();
+                                               int grandparent = copy->tree[parent].getParent();
+                                               int parentsSibIndex;
+                                               if (grandparent != -1) {
+                                                       int greatgrandparent = copy->tree[grandparent].getParent();
+                                                       int greatgrandparentLC = copy->tree[greatgrandparent].getLChild();
+                                                       int greatgrandparentRC = copy->tree[greatgrandparent].getRChild();
+                                                       
+                                                       int grandparentLC = copy->tree[grandparent].getLChild();
+                                                       int grandparentRC = copy->tree[grandparent].getRChild();
+                                                       
+                                                       parentsSibIndex = grandparentLC;
+                                                       if (grandparentLC == parent) { parentsSibIndex = grandparentRC; }
+
+                                                       //whichever of my greatgrandparents children was my grandparent
+                                                       if (greatgrandparentLC == grandparent) { greatgrandparentLC = parentsSibIndex; }
+                                                       else { greatgrandparentRC = parentsSibIndex; }
+                                                       
+                                                       copy->tree[parentsSibIndex].setParent(greatgrandparent);
+                                                       copy->tree[parentsSibIndex].setBranchLength((copy->tree[parentsSibIndex].getBranchLength()+copy->tree[grandparent].getBranchLength()));
+                                                       copy->tree[greatgrandparent].setChildren(greatgrandparentLC, greatgrandparentRC);
+                                               }else{
+                                                       copy->tree[parent].setChildren(-1, -1);
+                                                       cout << "issues with making subtree" << endl;
+                                               }
+                                               removedLeaves.insert(sibIndex);
+                                               removedLeaves.insert(i);
+                                       }
+                               }
+                       }
+                       }
+               }
+               
+               int root = 0;
+               for (int i = 0; i < copy->getNumNodes(); i++) {
+                       //you found the root
+                       if (copy->tree[i].getParent() == -1) { root = i; break; }
+               }
+               
+               int nextSpot = numLeaves;
+               populateNewTree(copy->tree, root, nextSpot);
+                               
        }
        catch(exception& e) {
-               cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function assembleTree. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+               m->errorOut(e, "Tree", "getCopy");
                exit(1);
        }
-       catch(...) {
-               cout << "An unknown error has occurred in the Tree class function assembleTree. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+}
+/*****************************************************************/
+int Tree::populateNewTree(vector<Node>& oldtree, int node, int& index) {
+       try {
+               
+               if (oldtree[node].getLChild() != -1) {
+                       int rc = populateNewTree(oldtree, oldtree[node].getLChild(), index);
+                       int lc = populateNewTree(oldtree, oldtree[node].getRChild(), index);
+
+                       tree[index].setChildren(lc, rc);
+                       tree[rc].setParent(index);
+                       tree[lc].setParent(index);
+                       
+                       tree[index].setBranchLength(oldtree[node].getBranchLength());
+                       tree[rc].setBranchLength(oldtree[oldtree[node].getLChild()].getBranchLength());
+                       tree[lc].setBranchLength(oldtree[oldtree[node].getRChild()].getBranchLength());
+                       
+                       return (index++);
+               }else { //you are a leaf
+                       int indexInNewTree = globaldata->gTreemap->getIndex(oldtree[node].getName());
+                       return indexInNewTree;
+               }
+       }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "populateNewTree");
                exit(1);
-       }               
+       }
 }
 /*****************************************************************/
 void Tree::getCopy(Tree* copy) {
@@ -137,15 +466,14 @@ void Tree::getCopy(Tree* copy) {
                        //copy pcount
                        tree[i].pcount = copy->tree[i].pcount;
                }
+               
+               groupNodeInfo = copy->groupNodeInfo;
+               
        }
        catch(exception& e) {
-               cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function getCopy. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+               m->errorOut(e, "Tree", "getCopy");
                exit(1);
        }
-       catch(...) {
-               cout << "An unknown error has occurred in the Tree class function getCopy. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
-               exit(1);
-       }               
 }
 /*****************************************************************/
 //returns a map with a groupname and the number of times that group was seen in the children
@@ -157,7 +485,7 @@ map<string, int> Tree::mergeGroups(int i) {
        try {
                int lc = tree[i].getLChild();
                int rc = tree[i].getRChild();
-               
+
                //set parsimony groups to left child
                map<string,int> parsimony = tree[lc].pGroups;
                
@@ -180,11 +508,10 @@ map<string, int> Tree::mergeGroups(int i) {
                // this is true if right child had a greater parsimony for a certain group
                if(maxPars > 1){
                        //erase all the groups that are only 1 because you found something with 2.
-                       for(it=parsimony.begin();it!=parsimony.end();it++){
+                       for(it=parsimony.begin();it!=parsimony.end();){
                                if(it->second == 1){
-                                       parsimony.erase(it->first);
-                                       it--;
-                               }
+                                       parsimony.erase(it++);
+                               }else { it++; }
                        }
                        //set one remaining groups to 1
                        //so with our above example p[white] = 2 would be left and it would become p[white] = 1
@@ -197,13 +524,9 @@ map<string, int> Tree::mergeGroups(int i) {
                return parsimony;
        }
        catch(exception& e) {
-               cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function mergeGroups. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+               m->errorOut(e, "Tree", "mergeGroups");
                exit(1);
        }
-       catch(...) {
-               cout << "An unknown error has occurred in the Tree class function mergeGroups. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
-               exit(1);
-       }               
 }
 /*****************************************************************/
 //returns a map with a groupname and the number of times that group was seen in the children
@@ -211,20 +534,24 @@ map<string, int> Tree::mergeGroups(int i) {
 // p[white] = 1 and p[black] = 1.  Now go up a level and merge that with a node who has p[white] = 1
 //and you get p[white] = 2, p[black] = 1, but you erase the p[black] because you have a p value higher than 1.
 
-map<string, int> Tree::mergeUserGroups(int i) {
+map<string, int> Tree::mergeUserGroups(int i, vector<string> g) {
        try {
        
                int lc = tree[i].getLChild();
                int rc = tree[i].getRChild();
                
                //loop through nodes groups removing the ones the user doesn't want
-               for (it = tree[lc].pGroups.begin(); it != tree[lc].pGroups.end(); it++) {
-                       if (inUsersGroups(it->first, globaldata->Groups) != true) { tree[lc].pGroups.erase(it->first); }
+               for(it=tree[lc].pGroups.begin();it!=tree[lc].pGroups.end();){
+                               if (m->inUsersGroups(it->first, g) != true) {
+                                       tree[lc].pGroups.erase(it++);
+                               }else { it++; }
                }
-               
+
                //loop through nodes groups removing the ones the user doesn't want
-               for (it = tree[rc].pGroups.begin(); it != tree[rc].pGroups.end(); it++) {
-                       if (inUsersGroups(it->first, globaldata->Groups) != true) { tree[rc].pGroups.erase(it->first); }
+               for(it=tree[rc].pGroups.begin();it!=tree[rc].pGroups.end();){
+                               if (m->inUsersGroups(it->first, g) != true) {
+                                       tree[rc].pGroups.erase(it++);
+                               }else { it++; }
                }
 
                //set parsimony groups to left child
@@ -245,34 +572,27 @@ map<string, int> Tree::mergeUserGroups(int i) {
                                maxPars = parsimony[it->first];
                        }
                }
-       
+                       
                // this is true if right child had a greater parsimony for a certain group
                if(maxPars > 1){
                        //erase all the groups that are only 1 because you found something with 2.
-                       for(it=parsimony.begin();it!=parsimony.end();it++){
+                       for(it=parsimony.begin();it!=parsimony.end();){
                                if(it->second == 1){
-                                       parsimony.erase(it->first);
-                                       it--;
-                               }
+                                       parsimony.erase(it++);
+                               }else { it++; }
                        }
-                       //set one remaining groups to 1
-                       //so with our above example p[white] = 2 would be left and it would become p[white] = 1
+
                        for(it=parsimony.begin();it!=parsimony.end();it++){
                                parsimony[it->first] = 1;
                        }
+               }               
                
-               }
-       
                return parsimony;
        }
        catch(exception& e) {
-               cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function mergeGroups. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+               m->errorOut(e, "Tree", "mergeUserGroups");
                exit(1);
        }
-       catch(...) {
-               cout << "An unknown error has occurred in the Tree class function mergeGroups. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
-               exit(1);
-       }               
 }
 
 
@@ -293,21 +613,19 @@ map<string,int> Tree::mergeGcounts(int position) {
                return sum;
        }
        catch(exception& e) {
-               cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function mergeGcounts. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+               m->errorOut(e, "Tree", "mergeGcounts");
                exit(1);
        }
-       catch(...) {
-               cout << "An unknown error has occurred in the Tree class function mergeGcounts. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
-               exit(1);
-       }               
 }
 /**************************************************************************************************/
 
-void Tree::randomLabels() {
+void Tree::randomLabels(vector<string> g) {
        try {
-               
-               //set up the groups the user wants to include
-               setGroups();
+       
+               //initialize groupNodeInfo
+               for (int i = 0; i < globaldata->gTreemap->namesOfGroups.size(); i++) {
+                       groupNodeInfo[globaldata->gTreemap->namesOfGroups[i]].resize(0);
+               }
                
                for(int i = 0; i < numLeaves; i++){
                        int z;
@@ -318,8 +636,8 @@ void Tree::randomLabels() {
                        //if either of the leaf nodes you are about to switch are not in the users groups then you don't want to switch them.
                        bool treez, treei;
                
-                       treez = inUsersGroups(tree[z].getGroup(), globaldata->Groups);
-                       treei = inUsersGroups(tree[i].getGroup(), globaldata->Groups);
+                       treez = m->inUsersGroups(tree[z].getGroup(), g);
+                       treei = m->inUsersGroups(tree[i].getGroup(), g);
                        
                        if ((treez == true) && (treei == true)) {
                                //switches node i and node z's info.
@@ -327,7 +645,7 @@ void Tree::randomLabels() {
                                tree[z].pGroups = (tree[i].pGroups);
                                tree[i].pGroups = (lib_hold);
                                
-                               string zgroup = tree[z].getGroup();
+                               vector<string> zgroup = tree[z].getGroup();
                                tree[z].setGroup(tree[i].getGroup());
                                tree[i].setGroup(zgroup);
                                
@@ -339,18 +657,17 @@ void Tree::randomLabels() {
                                tree[z].pcount = (tree[i].pcount);
                                tree[i].pcount = (gcount_hold);
                        }
+                       
+                       for (int k = 0; k < (tree[i].getGroup()).size(); k++) {  groupNodeInfo[(tree[i].getGroup())[k]].push_back(i); }
+                       for (int k = 0; k < (tree[z].getGroup()).size(); k++) {  groupNodeInfo[(tree[z].getGroup())[k]].push_back(z); }
                }
        }
        catch(exception& e) {
-               cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function randomLabels. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+               m->errorOut(e, "Tree", "randomLabels");
                exit(1);
        }
-       catch(...) {
-               cout << "An unknown error has occurred in the Tree class function randomLabels. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
-               exit(1);
-       }               
 }
-/**************************************************************************************************/
+/**************************************************************************************************
 
 void Tree::randomLabels(string groupA, string groupB) {
        try {
@@ -376,13 +693,9 @@ void Tree::randomLabels(string groupA, string groupB) {
                }
        }               
        catch(exception& e) {
-               cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function randomLabels. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+               m->errorOut(e, "Tree", "randomLabels");
                exit(1);
        }
-       catch(...) {
-               cout << "An unknown error has occurred in the Tree class function randomLabels. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
-               exit(1);
-       }               
 }
 /**************************************************************************************************/
 void Tree::randomBlengths()  {
@@ -396,23 +709,21 @@ void Tree::randomBlengths()  {
                }
        }
        catch(exception& e) {
-               cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function randomBlengths. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+               m->errorOut(e, "Tree", "randomBlengths");
                exit(1);
        }
-       catch(...) {
-               cout << "An unknown error has occurred in the Tree class function randomBlengths. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
-               exit(1);
-       }               
 }
 /*************************************************************************************************/
-void Tree::assembleRandomUnifracTree() {
-       randomLabels();
-       assembleTree();
+void Tree::assembleRandomUnifracTree(vector<string> g) {
+       randomLabels(g);
+       assembleTree("noNameCounts");
 }
 /*************************************************************************************************/
 void Tree::assembleRandomUnifracTree(string groupA, string groupB) {
-       randomLabels(groupA, groupB);
-       assembleTree();
+
+       vector<string> temp; temp.push_back(groupA); temp.push_back(groupB);
+       randomLabels(temp);
+       assembleTree("noNameCounts");
 }
 
 /*************************************************************************************************/
@@ -447,7 +758,7 @@ void Tree::randomTopology() {
                                        escape = 1;
                                }               
                        }
-               
+       
                        tree[i].setChildren(rnd_index1,rnd_index2);
                        tree[i].setParent(-1);
                        tree[rnd_index1].setParent(i);
@@ -455,38 +766,54 @@ void Tree::randomTopology() {
                }
        }
        catch(exception& e) {
-               cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function randomTopology. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+               m->errorOut(e, "Tree", "randomTopology");
+               exit(1);
+       }
+}
+/*****************************************************************/
+void Tree::print(ostream& out) {
+       try {
+               int root = findRoot();
+               printBranch(root, out, "branch");
+               out << ";" << endl;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "print");
                exit(1);
        }
-       catch(...) {
-               cout << "An unknown error has occurred in the Tree class function randomTopology. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+}
+/*****************************************************************/
+void Tree::print(ostream& out, string mode) {
+       try {
+               int root = findRoot();
+               printBranch(root, out, mode);
+               out << ";" << endl;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "print");
                exit(1);
-       }               
+       }
 }
-
 /*****************************************************************/
 // This prints out the tree in Newick form.
 void Tree::createNewickFile(string f) {
        try {
                int root = findRoot();
-               //filename = getRootName(globaldata->getTreeFile()) + "newick";
+               //filename = m->getRootName(globaldata->getTreeFile()) + "newick";
                filename = f;
-               openOutputFile(filename, out);
+
+               m->openOutputFile(filename, out);
                
-               printBranch(root);
+               printBranch(root, out, "branch");
                
                // you are at the end of the tree
                out << ";" << endl;
                out.close();
        }
        catch(exception& e) {
-               cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function createNewickFile. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+               m->errorOut(e, "Tree", "createNewickFile");
                exit(1);
        }
-       catch(...) {
-               cout << "An unknown error has occurred in the Tree class function createNewickFile. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
-               exit(1);
-       }               
 }
 
 /*****************************************************************/
@@ -497,85 +824,142 @@ int Tree::findRoot() {
                for (int i = 0; i < numNodes; i++) {
                        //you found the root
                        if (tree[i].getParent() == -1) { return i; }
+                       //cout << "i = " << i << endl;
+                       //cout << "i's parent = " << tree[i].getParent() << endl;  
                }
                return -1;
        }
        catch(exception& e) {
-               cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function findRoot. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+               m->errorOut(e, "Tree", "findRoot");
                exit(1);
        }
-       catch(...) {
-               cout << "An unknown error has occurred in the Tree class function findRoot. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
-               exit(1);
-       }               
 }
-
 /*****************************************************************/
-void Tree::printBranch(int node) {
-       try {
-               
-               // you are not a leaf
+void Tree::printBranch(int node, ostream& out, string mode) {
+try {
+
+// you are not a leaf
                if (tree[node].getLChild() != -1) {
                        out << "(";
-                       printBranch(tree[node].getLChild());
+                       printBranch(tree[node].getLChild(), out, mode);
                        out << ",";
-                       printBranch(tree[node].getRChild());
+                       printBranch(tree[node].getRChild(), out, mode);
                        out << ")";
+                       if (mode == "branch") {
+                               //if there is a branch length then print it
+                               if (tree[node].getBranchLength() != -1) {
+                                       out << ":" << tree[node].getBranchLength();
+                               }
+                       }else if (mode == "boot") {
+                               //if there is a label then print it
+                               if (tree[node].getLabel() != -1) {
+                                       out << tree[node].getLabel();
+                               }
+                       }else if (mode == "both") {
+                               if (tree[node].getLabel() != -1) {
+                                       out << tree[node].getLabel();
+                               }
+                               //if there is a branch length then print it
+                               if (tree[node].getBranchLength() != -1) {
+                                       out << ":" << tree[node].getBranchLength();
+                               }
+                       }
                }else { //you are a leaf
-                       out << tree[node].getGroup() << ":" << tree[node].getBranchLength();
+                       string leafGroup = globaldata->gTreemap->getGroup(tree[node].getName());
+                       
+                       if (mode == "branch") {
+                               out << leafGroup; 
+                               //if there is a branch length then print it
+                               if (tree[node].getBranchLength() != -1) {
+                                       out << ":" << tree[node].getBranchLength();
+                               }
+                       }else if (mode == "boot") {
+                               out << leafGroup; 
+                               //if there is a label then print it
+                               if (tree[node].getLabel() != -1) {
+                                       out << tree[node].getLabel();
+                               }
+                       }else if (mode == "both") {
+                               out << tree[node].getName();
+                               if (tree[node].getLabel() != -1) {
+                                       out << tree[node].getLabel();
+                               }
+                               //if there is a branch length then print it
+                               if (tree[node].getBranchLength() != -1) {
+                                       out << ":" << tree[node].getBranchLength();
+                               }
+                       }
                }
                
        }
        catch(exception& e) {
-               cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function printBranch. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+               m->errorOut(e, "Tree", "printBranch");
                exit(1);
        }
-       catch(...) {
-               cout << "An unknown error has occurred in the Tree class function printBranch. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
-               exit(1);
-       }               
 }
-
 /*****************************************************************/
-
-void Tree::setGroups() {
+void Tree::printBranch(int node, ostream& out, string mode, vector<Node>& theseNodes) {
        try {
-               //if the user has not entered specific groups to analyze then do them all
-               if (globaldata->Groups.size() != 0) {
-                       //check that groups are valid
-                       for (int i = 0; i < globaldata->Groups.size(); i++) {
-                               if (globaldata->gTreemap->isValidGroup(globaldata->Groups[i]) != true) {
-                                       cout << globaldata->Groups[i] << " is not a valid group, and will be disregarded." << endl;
-                                       // erase the invalid group from globaldata->Groups
-                                       globaldata->Groups.erase (globaldata->Groups.begin()+i);
+               
+               // you are not a leaf
+               if (theseNodes[node].getLChild() != -1) {
+                       out << "(";
+                       printBranch(theseNodes[node].getLChild(), out, mode);
+                       out << ",";
+                       printBranch(theseNodes[node].getRChild(), out, mode);
+                       out << ")";
+                       if (mode == "branch") {
+                               //if there is a branch length then print it
+                               if (theseNodes[node].getBranchLength() != -1) {
+                                       out << ":" << theseNodes[node].getBranchLength();
+                               }
+                       }else if (mode == "boot") {
+                               //if there is a label then print it
+                               if (theseNodes[node].getLabel() != -1) {
+                                       out << theseNodes[node].getLabel();
+                               }
+                       }else if (mode == "both") {
+                               if (theseNodes[node].getLabel() != -1) {
+                                       out << theseNodes[node].getLabel();
+                               }
+                               //if there is a branch length then print it
+                               if (theseNodes[node].getBranchLength() != -1) {
+                                       out << ":" << theseNodes[node].getBranchLength();
                                }
                        }
+               }else { //you are a leaf
+                       string leafGroup = globaldata->gTreemap->getGroup(theseNodes[node].getName());
                        
-                       //if the user only entered invalid groups
-                       if (globaldata->Groups.size() == 0) { 
-                               cout << "When using the groups parameter you must have at least 1 valid group. I will run the command using all the groups in your groupfile." << endl; 
-                               for (int i = 0; i < globaldata->gTreemap->namesOfGroups.size(); i++) {
-                                       globaldata->Groups.push_back(globaldata->gTreemap->namesOfGroups[i]);
+                       if (mode == "branch") {
+                               out << leafGroup; 
+                               //if there is a branch length then print it
+                               if (theseNodes[node].getBranchLength() != -1) {
+                                       out << ":" << theseNodes[node].getBranchLength();
+                               }
+                       }else if (mode == "boot") {
+                               out << leafGroup; 
+                               //if there is a label then print it
+                               if (theseNodes[node].getLabel() != -1) {
+                                       out << theseNodes[node].getLabel();
+                               }
+                       }else if (mode == "both") {
+                               out << theseNodes[node].getName();
+                               if (theseNodes[node].getLabel() != -1) {
+                                       out << theseNodes[node].getLabel();
+                               }
+                               //if there is a branch length then print it
+                               if (theseNodes[node].getBranchLength() != -1) {
+                                       out << ":" << theseNodes[node].getBranchLength();
                                }
-                       }
-                                       
-               }else {
-                       for (int i = 0; i < globaldata->gTreemap->namesOfGroups.size(); i++) {
-                               globaldata->Groups.push_back(globaldata->gTreemap->namesOfGroups[i]);
                        }
                }
+               
        }
        catch(exception& e) {
-               cout << "Standard Error: " << e.what() << " has occurred in the Tree class Function setGroups. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+               m->errorOut(e, "Tree", "printBranch");
                exit(1);
        }
-       catch(...) {
-               cout << "An unknown error has occurred in the Tree class function setGroups. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
-               exit(1);
-       }               
-
 }
-
 /*****************************************************************/
 
 void Tree::printTree() {
@@ -588,5 +972,159 @@ void Tree::printTree() {
 }
 
 /*****************************************************************/
+//this code is a mess and should be rethought...-slw
+void Tree::parseTreeFile() {
+       
+       //only takes names from the first tree and assumes that all trees use the same names.
+       try {
+               string filename = globaldata->getTreeFile();
+               ifstream filehandle;
+               m->openInputFile(filename, filehandle);
+               int c, comment;
+               comment = 0;
+               int done = 1;
+               
+               //ifyou are not a nexus file 
+               if((c = filehandle.peek()) != '#') {  
+                       while((c = filehandle.peek()) != ';') { 
+                               while ((c = filehandle.peek()) != ';') {
+                                       // get past comments
+                                       if(c == '[') {
+                                               comment = 1;
+                                       }
+                                       if(c == ']'){
+                                               comment = 0;
+                                       }
+                                       if((c == '(') && (comment != 1)){ break; }
+                                       filehandle.get();
+                               }
+
+                               done = readTreeString(filehandle); 
+                               if (done == 0) { break; }
+                       }
+               //ifyou are a nexus file
+               }else if((c = filehandle.peek()) == '#') {
+                       string holder = "";
+                                       
+                       // get past comments
+                       while(holder != "translate" && holder != "Translate"){  
+                               if(holder == "[" || holder == "[!"){
+                                       comment = 1;
+                               }
+                               if(holder == "]"){
+                                       comment = 0;
+                               }
+                               filehandle >> holder; 
+
+                               //if there is no translate then you must read tree string otherwise use translate to get names
+                               if((holder == "tree") && (comment != 1)){       
+                                       //pass over the "tree rep.6878900 = "
+                                       while (((c = filehandle.get()) != '(') && ((c = filehandle.peek()) != EOF)) {;}
+
+                                       if(c == EOF) { break; }
+                                       filehandle.putback(c);  //put back first ( of tree.
+                                       done = readTreeString(filehandle);
+       
+                                       break;
+                               }
+                       
+                               if (done == 0) { break;  }
+                       }
+                       
+                       //use nexus translation rather than parsing tree to save time
+                       if((holder == "translate") || (holder == "Translate")) {
+
+                               string number, name, h;
+                               h = ""; // so it enters the loop the first time
+                               while((h != ";") && (number != ";")) { 
+                                       filehandle >> number;
+                                       filehandle >> name;
+       
+                                       //c = , until done with translation then c = ;
+                                       h = name.substr(name.length()-1, name.length()); 
+                                       name.erase(name.end()-1);  //erase the comma
+                                       globaldata->Treenames.push_back(number);
+                               }
+                               if(number == ";") { globaldata->Treenames.pop_back(); }  //in case ';' from translation is on next line instead of next to last name
+                       }
+               }
+               filehandle.close();
+               
+               //for (int i = 0; i < globaldata->Treenames.size(); i++) {
+//cout << globaldata->Treenames[i] << endl; }
+//cout << globaldata->Treenames.size() << endl;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "parseTreeFile");
+               exit(1);
+       }
+}
+/*******************************************************/
+
+/*******************************************************/
+int Tree::readTreeString(ifstream& filehandle) {
+       try {
+               int c;
+               string name;  //, k
+               
+               while((c = filehandle.peek()) != ';') { 
+//k = c;
+//cout << " at beginning of while " <<  k << endl;                     
+                       if(c == ')')  {    
+                               //to pass over labels in trees
+                               c=filehandle.get();
+                               while((c!=',') && (c != -1) && (c!= ':') && (c!=';')){ c=filehandle.get(); }
+                               filehandle.putback(c);
+                       }
+                       if(c == ';') { return 0; }
+                       if(c == -1) { return 0; }
+                       //if you are a name
+                       if((c != '(') && (c != ')') && (c != ',') && (c != ':') && (c != '\n') && (c != '\t') && (c != 32)) { //32 is space
+                               name = "";
+                               c = filehandle.get();
+                       //k = c;
+//cout << k << endl;
+                               while ((c != '(') && (c != ')') && (c != ',') && (c != ':') && (c != '\n') && (c != 32) && (c != '\t')) {                       
+                                       name += c;
+                                       c = filehandle.get();
+                       //k = c;
+//cout << " in name while " << k << endl;
+                               }
+                               
+//cout << "name = " << name << endl;
+                               globaldata->Treenames.push_back(name);
+                               filehandle.putback(c);
+//k = c;
+//cout << " after putback" <<  k << endl;
+                       } 
+                       
+                       if(c  == ':') { //read until you reach the end of the branch length
+                               while ((c != '(') && (c != ')') && (c != ',') && (c != ';') && (c != '\n') && (c != '\t') && (c != 32)) {
+                                       c = filehandle.get();
+       //k = c;
+       //cout << " in branch while " << k << endl;
+                               }
+                               filehandle.putback(c);
+                       }
+               
+                       c = filehandle.get();
+//k = c;
+       //cout << " here after get " << k << endl;
+                       if(c == ';') { return 0; }
+                       if(c == ')') { filehandle.putback(c); }
+       //k = c;
+//cout << k << endl;
+
+               }
+               return 0;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "Tree", "readTreeString");
+               exit(1);
+       }
+}      
+
+/*******************************************************/
 
+/*******************************************************/