]> git.donarmstrong.com Git - mothur.git/blobdiff - summarysharedcommand.cpp
fixes while testing 1.33.0
[mothur.git] / summarysharedcommand.cpp
index 77db937c6bfb325bdcb133848103d14b639155fe..59c12a03dae958f4bb199a6aad94056264c0426a 100644 (file)
@@ -8,60 +8,23 @@
  */
 
 #include "summarysharedcommand.h"
-#include "sharedsobscollectsummary.h"
-#include "sharedchao1.h"
-#include "sharedace.h"
-#include "sharednseqs.h"
-#include "sharedjabund.h"
-#include "sharedsorabund.h"
-#include "sharedjclass.h"
-#include "sharedsorclass.h"
-#include "sharedjest.h"
-#include "sharedsorest.h"
-#include "sharedthetayc.h"
-#include "sharedthetan.h"
-#include "sharedkstest.h"
-#include "whittaker.h"
-#include "sharedochiai.h"
-#include "sharedanderbergs.h"
-#include "sharedkulczynski.h"
-#include "sharedkulczynskicody.h"
-#include "sharedlennon.h"
-#include "sharedmorisitahorn.h"
-#include "sharedbraycurtis.h"
-#include "sharedjackknife.h"
-#include "whittaker.h"
-#include "odum.h"
-#include "canberra.h"
-#include "structeuclidean.h"
-#include "structchord.h"
-#include "hellinger.h"
-#include "manhattan.h"
-#include "structpearson.h"
-#include "soergel.h"
-#include "spearman.h"
-#include "structkulczynski.h"
-#include "structchi2.h"
-#include "speciesprofile.h"
-#include "hamming.h"
-#include "gower.h"
-#include "memchi2.h"
-#include "memchord.h"
-#include "memeuclidean.h"
-#include "mempearson.h"
+#include "subsample.h"
 
 //**********************************************************************************************************************
 vector<string> SummarySharedCommand::setParameters(){  
        try {
-               CommandParameter pshared("shared", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(pshared);
-               CommandParameter plabel("label", "String", "", "", "", "", "",false,false); parameters.push_back(plabel);
-               CommandParameter pdistance("distance", "Boolean", "", "F", "", "", "",false,false); parameters.push_back(pdistance);
-               CommandParameter pcalc("calc", "Multiple", "sharedchao-sharedsobs-sharedace-jabund-sorabund-jclass-sorclass-jest-sorest-thetayc-thetan-kstest-whittaker-sharednseqs-ochiai-anderberg-kulczynski-kulczynskicody-lennon-morisitahorn-braycurtis-odum-canberra-structeuclidean-structchord-hellinger-manhattan-structpearson-soergel-spearman-structkulczynski-speciesprofile-structchi2-hamming-gower-memchi2-memchord-memeuclidean-mempearson", "sharedsobs-sharedchao-sharedace-jabund-sorabund-jclass-sorclass-jest-sorest-thetayc-thetan", "", "", "",true,false); parameters.push_back(pcalc);
-               CommandParameter pall("all", "Boolean", "", "F", "", "", "",false,false); parameters.push_back(pall);
-               CommandParameter pprocessors("processors", "Number", "", "1", "", "", "",false,false); parameters.push_back(pprocessors);
-               CommandParameter pgroups("groups", "String", "", "", "", "", "",false,false); parameters.push_back(pgroups);
-               CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
-               CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir);
+               CommandParameter pshared("shared", "InputTypes", "", "", "none", "none", "none","summary",false,true,true); parameters.push_back(pshared);
+               CommandParameter plabel("label", "String", "", "", "", "", "","",false,false); parameters.push_back(plabel);
+        CommandParameter psubsample("subsample", "String", "", "", "", "", "","phylip",false,false); parameters.push_back(psubsample);
+               CommandParameter pdistance("distance", "Boolean", "", "F", "", "", "","phylip",false,false); parameters.push_back(pdistance);
+               CommandParameter pcalc("calc", "Multiple", "sharedchao-sharedsobs-sharedace-jabund-sorabund-jclass-sorclass-jest-sorest-thetayc-thetan-kstest-whittaker-sharednseqs-ochiai-anderberg-kulczynski-kulczynskicody-lennon-morisitahorn-braycurtis-odum-canberra-structeuclidean-structchord-hellinger-manhattan-structpearson-soergel-spearman-structkulczynski-speciesprofile-structchi2-hamming-gower-memchi2-memchord-memeuclidean-mempearson-jsd-rjsd", "sharedsobs-sharedchao-sharedace-jabund-sorabund-jclass-sorclass-jest-sorest-thetayc-thetan", "", "", "","",true,false,true); parameters.push_back(pcalc);
+        CommandParameter poutput("output", "Multiple", "lt-square", "lt", "", "", "","",false,false); parameters.push_back(poutput);
+               CommandParameter pall("all", "Boolean", "", "F", "", "", "","",false,false); parameters.push_back(pall);
+        CommandParameter piters("iters", "Number", "", "1000", "", "", "","",false,false); parameters.push_back(piters);
+               CommandParameter pprocessors("processors", "Number", "", "1", "", "", "","",false,false,true); parameters.push_back(pprocessors);
+               CommandParameter pgroups("groups", "String", "", "", "", "", "","",false,false); parameters.push_back(pgroups);
+               CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
+               CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
                
                vector<string> myArray;
                for (int i = 0; i < parameters.size(); i++) {   myArray.push_back(parameters[i].name);          }
@@ -77,11 +40,14 @@ string SummarySharedCommand::getHelpString(){
        try {
                string helpString = "";
                ValidCalculators validCalculator;
-               helpString += "The summary.shared command parameters are shared, label, calc, distance, processors and all.  shared is required if there is no current sharedfile.\n";
+               helpString += "The summary.shared command parameters are shared, label, calc, distance, processors, subsample, iters and all.  shared is required if there is no current sharedfile.\n";
                helpString += "The summary.shared command should be in the following format: \n";
                helpString += "summary.shared(label=yourLabel, calc=yourEstimators, groups=yourGroups).\n";
                helpString += "Example summary.shared(label=unique-.01-.03, groups=B-C, calc=sharedchao-sharedace-jabund-sorensonabund-jclass-sorclass-jest-sorest-thetayc-thetan).\n";
                helpString +=  validCalculator.printCalc("sharedsummary");
+        helpString += "The iters parameter allows you to choose the number of times you would like to run the subsample.\n";
+        helpString += "The subsample parameter allows you to enter the size pergroup of the sample or you can set subsample=T and mothur will use the size of your smallest group.\n";
+        helpString += "The output parameter allows you to specify format of your distance matrix. Options are lt, and square. The default is lt.\n";
                helpString += "The default value for calc is sharedsobs-sharedchao-sharedace-jabund-sorensonabund-jclass-sorclass-jest-sorest-thetayc-thetan\n";
                helpString += "The default value for groups is all the groups in your groupfile.\n";
                helpString += "The distance parameter allows you to indicate you would like a distance file created for each calculator for each label, default=f.\n";
@@ -98,12 +64,29 @@ string SummarySharedCommand::getHelpString(){
        }
 }
 //**********************************************************************************************************************
+string SummarySharedCommand::getOutputPattern(string type) {
+    try {
+        string pattern = "";
+        
+        if (type == "summary") {  pattern = "[filename],summary-[filename],[tag],summary"; } 
+        else if (type == "phylip") {  pattern = "[filename],[calc],[distance],[outputtag],[tag2],dist"; } 
+        else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->control_pressed = true;  }
+        
+        return pattern;
+    }
+    catch(exception& e) {
+        m->errorOut(e, "SummarySharedCommand", "getOutputPattern");
+        exit(1);
+    }
+}
+//**********************************************************************************************************************
 SummarySharedCommand::SummarySharedCommand(){  
        try {
                abort = true; calledHelp = true; 
                setParameters();
                vector<string> tempOutNames;
                outputTypes["summary"] = tempOutNames;
+        outputTypes["phylip"] = tempOutNames;
        }
        catch(exception& e) {
                m->errorOut(e, "SummarySharedCommand", "SummarySharedCommand");
@@ -138,6 +121,7 @@ SummarySharedCommand::SummarySharedCommand(string option)  {
                        //initialize outputTypes
                        vector<string> tempOutNames;
                        outputTypes["summary"] = tempOutNames;
+            outputTypes["phylip"] = tempOutNames;
                        
                        //if the user changes the input directory command factory will send this info to us in the output parameter 
                        string inputDir = validParameter.validFile(parameters, "inputdir", false);              
@@ -194,18 +178,36 @@ SummarySharedCommand::SummarySharedCommand(string option)  {
                        if (groups == "not found") { groups = ""; }
                        else { 
                                m->splitAtDash(groups, Groups);
-                               m->Groups = Groups;
+                               m->setGroups(Groups);
                        }
                        
                        string temp = validParameter.validFile(parameters, "all", false);                               if (temp == "not found") { temp = "false"; }
                        all = m->isTrue(temp);
                        
-                       temp = validParameter.validFile(parameters, "distance", false);                                 if (temp == "not found") { temp = "false"; }
+            temp = validParameter.validFile(parameters, "iters", false);                       if (temp == "not found") { temp = "1000"; }
+                       m->mothurConvert(temp, iters); 
+            
+            output = validParameter.validFile(parameters, "output", false);            
+            if(output == "not found"){ output = "lt"; }
+            else { createPhylip = true; }
+                       if ((output != "lt") && (output != "square")) { m->mothurOut(output + " is not a valid output form. Options are lt and square. I will use lt."); m->mothurOutEndLine(); output = "lt"; }
+            
+            temp = validParameter.validFile(parameters, "subsample", false);           if (temp == "not found") { temp = "F"; }
+                       if (m->isNumeric1(temp)) { m->mothurConvert(temp, subsampleSize); subsample = true; }
+            else {  
+                if (m->isTrue(temp)) { subsample = true; subsampleSize = -1; }  //we will set it to smallest group later 
+                else { subsample = false; }
+            }
+            
+            if (subsample == false) { iters = 0; }
+            
+            temp = validParameter.validFile(parameters, "distance", false);                                    if (temp == "not found") { temp = "false"; }
                        createPhylip = m->isTrue(temp);
-                       
+            if (subsample) { createPhylip = true; }
+            
                        temp = validParameter.validFile(parameters, "processors", false);       if (temp == "not found"){       temp = m->getProcessors();      }
                        m->setProcessors(temp);
-                       convert(temp, processors); 
+                       m->mothurConvert(temp, processors); 
                        
                        if (abort == false) {
                        
@@ -292,6 +294,10 @@ SummarySharedCommand::SummarySharedCommand(string option)  {
                                                        sumCalculators.push_back(new MemEuclidean());
                                                }else if (Estimators[i] == "mempearson") { 
                                                        sumCalculators.push_back(new MemPearson());
+                                               }else if (Estimators[i] == "jsd") {
+                                                       sumCalculators.push_back(new JSD());
+                                               }else if (Estimators[i] == "rjsd") {
+                                                       sumCalculators.push_back(new RJSD());
                                                }
                                        }
                                }
@@ -313,7 +319,9 @@ int SummarySharedCommand::execute(){
                if (abort == true) { if (calledHelp) { return 0; }  return 2;   }
                
                ofstream outputFileHandle, outAll;
-               string outputFileName = outputDir + m->getRootName(m->getSimpleName(sharedfile)) + "shared.summary";
+        map<string, string> variables; 
+               variables["[filename]"] = outputDir + m->getRootName(m->getSimpleName(sharedfile));
+               string outputFileName = getOutputFileName("summary",variables);
                
                //if the users entered no valid calculators don't execute command
                if (sumCalculators.size() == 0) { return 0; }
@@ -344,7 +352,8 @@ int SummarySharedCommand::execute(){
                outputFileHandle.close();
                
                //create file and put column headers for multiple groups file
-               string outAllFileName = ((m->getRootName(sharedfile)) + "sharedmultiple.summary");
+        variables["[tag]"]= "multiple";
+               string outAllFileName = getOutputFileName("summary",variables);
                if (mult == true) {
                        m->openOutputFile(outAllFileName, outAll);
                        outputNames.push_back(outAllFileName);
@@ -380,15 +389,42 @@ int SummarySharedCommand::execute(){
                        delete input;
                        for (int i = 0; i < lookup.size(); i++) { delete lookup[i]; }
                        for(int i=0;i<sumCalculators.size();i++){  delete sumCalculators[i]; }
-                       m->Groups.clear(); 
+                       m->clearGroups(); 
                        return 0;
                }
                /******************************************************/
-               
+        if (subsample) { 
+            if (subsampleSize == -1) { //user has not set size, set size = smallest samples size
+                subsampleSize = lookup[0]->getNumSeqs();
+                for (int i = 1; i < lookup.size(); i++) {
+                    int thisSize = lookup[i]->getNumSeqs();
+                    
+                    if (thisSize < subsampleSize) {    subsampleSize = thisSize;       }
+                }
+            }else {
+                m->clearGroups();
+                Groups.clear();
+                vector<SharedRAbundVector*> temp;
+                for (int i = 0; i < lookup.size(); i++) {
+                    if (lookup[i]->getNumSeqs() < subsampleSize) { 
+                        m->mothurOut(lookup[i]->getGroup() + " contains " + toString(lookup[i]->getNumSeqs()) + ". Eliminating."); m->mothurOutEndLine();
+                        delete lookup[i];
+                    }else { 
+                        Groups.push_back(lookup[i]->getGroup()); 
+                        temp.push_back(lookup[i]);
+                    }
+                } 
+                lookup = temp;
+                m->setGroups(Groups);
+            }
+            
+            if (lookup.size() < 2) { m->mothurOut("You have not provided enough valid groups.  I cannot run the command."); m->mothurOutEndLine(); m->control_pressed = true; delete input; return 0; }
+        }
+
                
                /******************************************************/
                //comparison breakup to be used by different processes later
-               numGroups = m->Groups.size();
+               numGroups = lookup.size();
                lines.resize(processors);
                for (int i = 0; i < processors; i++) {
                        lines[i].start = int (sqrt(float(i)/float(processors)) * numGroups);
@@ -408,7 +444,7 @@ int SummarySharedCommand::execute(){
                                delete input; 
                                for (int i = 0; i < lookup.size(); i++) { delete lookup[i]; }
                                for(int i=0;i<sumCalculators.size();i++){  delete sumCalculators[i]; }
-                               m->Groups.clear(); 
+                               m->clearGroups(); 
                                return 0;
                        }
 
@@ -450,7 +486,7 @@ int SummarySharedCommand::execute(){
                        m->mothurRemove(outputFileName); 
                        delete input; 
                        for(int i=0;i<sumCalculators.size();i++){  delete sumCalculators[i]; }
-                       m->Groups.clear(); 
+                       m->clearGroups(); 
                        return 0;
                }
 
@@ -479,7 +515,7 @@ int SummarySharedCommand::execute(){
                
                                
                //reset groups parameter
-               m->Groups.clear();  
+               m->clearGroups();  
                
                for(int i=0;i<sumCalculators.size();i++){  delete sumCalculators[i]; }
                delete input;  
@@ -504,157 +540,343 @@ int SummarySharedCommand::execute(){
                exit(1);
        }
 }
-
 /***********************************************************/
-int SummarySharedCommand::process(vector<SharedRAbundVector*> thisLookup, string sumFileName, string sumAllFileName) {
+int SummarySharedCommand::printSims(ostream& out, vector< vector<double> >& simMatrix) {
        try {
-                       vector< vector<seqDist> > calcDists;  //vector containing vectors that contains the summary results for each group compare
-                       calcDists.resize(sumCalculators.size()); //one for each calc, this will be used to make .dist files
-                               
-                       #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
-                               if(processors == 1){
-                                       driver(thisLookup, 0, numGroups, sumFileName+".temp", sumAllFileName+".temp", calcDists);
-                                       m->appendFiles((sumFileName + ".temp"), sumFileName);
-                                       m->mothurRemove((sumFileName + ".temp"));
-                                       if (mult) {
-                                               m->appendFiles((sumAllFileName + ".temp"), sumAllFileName);
-                                               m->mothurRemove((sumAllFileName + ".temp"));
-                                       }
-                               }else{
-                                       int process = 1;
-                                       vector<int> processIDS;
                
-                                       //loop through and create all the processes you want
-                                       while (process != processors) {
-                                               int pid = fork();
-                                               
-                                               if (pid > 0) {
-                                                       processIDS.push_back(pid); 
-                                                       process++;
-                                               }else if (pid == 0){
-                                                       driver(thisLookup, lines[process].start, lines[process].end, sumFileName + toString(getpid()) + ".temp", sumAllFileName + toString(getpid()) + ".temp", calcDists);   
-                                                       
-                                                       //only do this if you want a distance file
-                                                       if (createPhylip) {
-                                                               string tempdistFileName = m->getRootName(m->getSimpleName(sumFileName)) + toString(getpid()) + ".dist";
-                                                               ofstream outtemp;
-                                                               m->openOutputFile(tempdistFileName, outtemp);
-                                                               
-                                                               for (int i = 0; i < calcDists.size(); i++) {
-                                                                       outtemp << calcDists[i].size() << endl;
-                                                                       
-                                                                       for (int j = 0; j < calcDists[i].size(); j++) {
-                                                                               outtemp << calcDists[i][j].seq1 << '\t' << calcDists[i][j].seq2 << '\t' << calcDists[i][j].dist << endl;
-                                                                       }
-                                                               }
-                                                               outtemp.close();
-                                                       }
-                                                       
-                                                       exit(0);
-                                               }else { 
-                                                       m->mothurOut("[ERROR]: unable to spawn the necessary processes."); m->mothurOutEndLine(); 
-                                                       for (int i = 0; i < processIDS.size(); i++) { kill (processIDS[i], SIGINT); }
-                                                       exit(0);
-                                               }
-                                       }
-                                       
-                                       //parent do your part
-                                       driver(thisLookup, lines[0].start, lines[0].end, sumFileName + toString(getpid()) + ".temp", sumAllFileName + toString(getpid()) + ".temp", calcDists);   
-                                       m->appendFiles((sumFileName + toString(getpid()) + ".temp"), sumFileName);
-                                       m->mothurRemove((sumFileName + toString(getpid()) + ".temp"));
-                                       if (mult) { m->appendFiles((sumAllFileName + toString(getpid()) + ".temp"), sumAllFileName); }
-                                               
-                                       //force parent to wait until all the processes are done
-                                       for (int i = 0; i < processIDS.size(); i++) {
-                                               int temp = processIDS[i];
-                                               wait(&temp);
-                                       }
-                                       
-                                       for (int i = 0; i < processIDS.size(); i++) {
-                                               m->appendFiles((sumFileName + toString(processIDS[i]) + ".temp"), sumFileName);
-                                               m->mothurRemove((sumFileName + toString(processIDS[i]) + ".temp"));
-                                               if (mult) {     m->mothurRemove((sumAllFileName + toString(processIDS[i]) + ".temp"));  }
-                                               
-                                               if (createPhylip) {
-                                                       string tempdistFileName = m->getRootName(m->getSimpleName(sumFileName)) + toString(processIDS[i]) +  ".dist";
-                                                       ifstream intemp;
-                                                       m->openInputFile(tempdistFileName, intemp);
-                                                       
-                                                       for (int k = 0; k < calcDists.size(); k++) {
-                                                               int size = 0;
-                                                               intemp >> size; m->gobble(intemp);
-                                                                       
-                                                               for (int j = 0; j < size; j++) {
-                                                                       int seq1 = 0;
-                                                                       int seq2 = 0;
-                                                                       float dist = 1.0;
-                                                                       
-                                                                       intemp >> seq1 >> seq2 >> dist;   m->gobble(intemp);
-                                                                       
-                                                                       seqDist tempDist(seq1, seq2, dist);
-                                                                       calcDists[k].push_back(tempDist);
-                                                               }
-                                                       }
-                                                       intemp.close();
-                                                       m->mothurRemove(tempdistFileName);
-                                               }
-                                       }
-
-                               }
-                       #else
-                               driver(thisLookup, 0, numGroups, (sumFileName + ".temp"), (sumAllFileName + ".temp"), calcDists);
-                               m->appendFiles((sumFileName + ".temp"), sumFileName);
-                               m->mothurRemove((sumFileName + ".temp"));
-                               if (mult) {
-                                       m->appendFiles((sumAllFileName + ".temp"), sumAllFileName);
-                                       m->mothurRemove((sumAllFileName + ".temp"));
+               out.setf(ios::fixed, ios::floatfield); out.setf(ios::showpoint);
+               
+               //output num seqs
+               out << simMatrix.size() << endl;
+               
+               if (output == "lt") {
+                       for (int b = 0; b < simMatrix.size(); b++)      {
+                               out << lookup[b]->getGroup() << '\t';
+                               for (int n = 0; n < b; n++)     {
+                    if (m->control_pressed) { return 0; }
+                                       out << simMatrix[b][n] << '\t'; 
                                }
-                       #endif
-                       
-                       if (createPhylip) {
-                               for (int i = 0; i < calcDists.size(); i++) {
-                                       if (m->control_pressed) { break; }
-                               
-                                       string distFileName = outputDir + m->getRootName(m->getSimpleName(sumFileName)) + sumCalculators[i]->getName() + "." + thisLookup[0]->getLabel() + ".dist";
-                                       outputNames.push_back(distFileName);
-                                       ofstream outDist;
-                                       m->openOutputFile(distFileName, outDist);
-                                       outDist.setf(ios::fixed, ios::floatfield); outDist.setf(ios::showpoint);
-                                       
-                                       //initialize matrix
-                                       vector< vector<float> > matrix; //square matrix to represent the distance
-                                       matrix.resize(thisLookup.size());
-                                       for (int k = 0; k < thisLookup.size(); k++) {  matrix[k].resize(thisLookup.size(), 0.0); }
-                                       
-                                       
-                                       for (int j = 0; j < calcDists[i].size(); j++) {
-                                               int row = calcDists[i][j].seq1;
-                                               int column = calcDists[i][j].seq2;
-                                               float dist = calcDists[i][j].dist;
-                                               
-                                               matrix[row][column] = dist;
-                                               matrix[column][row] = dist;
-                                       }
-                                       
-                                       //output to file
-                                       outDist << thisLookup.size() << endl;
-                                       for (int r=0; r<thisLookup.size(); r++) { 
-                                               //output name
-                                               string name = thisLookup[r]->getGroup();
-                                               if (name.length() < 10) { //pad with spaces to make compatible
-                                                       while (name.length() < 10) {  name += " ";  }
-                                               }
-                                               outDist << name << '\t';
-                                       
-                                               //output distances
-                                               for (int l = 0; l < r; l++) {   outDist  << matrix[r][l] << '\t';  }
-                                               outDist << endl;
-                                       }
-                                       
-                                       outDist.close();
+                               out << endl;
+                       }
+               }else{
+                       for (int b = 0; b < simMatrix.size(); m++)      {
+                               out << lookup[b]->getGroup() << '\t';
+                               for (int n = 0; n < simMatrix[b].size(); n++)   {
+                    if (m->control_pressed) { return 0; }
+                                       out << simMatrix[b][n] << '\t'; 
                                }
+                               out << endl;
                        }
-               return 0;
+               }
+        
+        return 0;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "SummarySharedCommand", "printSims");
+               exit(1);
+       }
+}
+/***********************************************************/
+int SummarySharedCommand::process(vector<SharedRAbundVector*> thisLookup, string sumFileName, string sumAllFileName) {
+       try {
+        vector< vector< vector<seqDist> > > calcDistsTotals;  //each iter, one for each calc, then each groupCombos dists. this will be used to make .dist files
+        vector< vector<seqDist>  > calcDists; calcDists.resize(sumCalculators.size());                 
+        
+        for (int thisIter = 0; thisIter < iters+1; thisIter++) {
+            
+            vector<SharedRAbundVector*> thisItersLookup = thisLookup;
+            
+            if (subsample && (thisIter != 0)) { //we want the summary results for the whole dataset, then the subsampling
+                SubSample sample;
+                vector<string> tempLabels; //dont need since we arent printing the sampled sharedRabunds
+                
+                //make copy of lookup so we don't get access violations
+                vector<SharedRAbundVector*> newLookup;
+                for (int k = 0; k < thisItersLookup.size(); k++) {
+                    SharedRAbundVector* temp = new SharedRAbundVector();
+                    temp->setLabel(thisItersLookup[k]->getLabel());
+                    temp->setGroup(thisItersLookup[k]->getGroup());
+                    newLookup.push_back(temp);
+                }
+                
+                //for each bin
+                for (int k = 0; k < thisItersLookup[0]->getNumBins(); k++) {
+                    if (m->control_pressed) { for (int j = 0; j < newLookup.size(); j++) {  delete newLookup[j];  } return 0; }
+                    for (int j = 0; j < thisItersLookup.size(); j++) { newLookup[j]->push_back(thisItersLookup[j]->getAbundance(k), thisItersLookup[j]->getGroup()); }
+                }
+                
+                tempLabels = sample.getSample(newLookup, subsampleSize);
+                thisItersLookup = newLookup;
+            }
+        
+            
+            if(processors == 1){
+                driver(thisLookup, 0, numGroups, sumFileName+".temp", sumAllFileName+".temp", calcDists);
+                m->appendFiles((sumFileName + ".temp"), sumFileName);
+                m->mothurRemove((sumFileName + ".temp"));
+                if (mult) {
+                    m->appendFiles((sumAllFileName + ".temp"), sumAllFileName);
+                    m->mothurRemove((sumAllFileName + ".temp"));
+                }
+            }else{
+                
+                int process = 1;
+                vector<int> processIDS;
+                
+#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
+                //loop through and create all the processes you want
+                while (process != processors) {
+                    int pid = fork();
+                    
+                    if (pid > 0) {
+                        processIDS.push_back(pid); 
+                        process++;
+                    }else if (pid == 0){
+                        driver(thisLookup, lines[process].start, lines[process].end, sumFileName + toString(getpid()) + ".temp", sumAllFileName + toString(getpid()) + ".temp", calcDists);   
+                        
+                        //only do this if you want a distance file
+                        if (createPhylip) {
+                            string tempdistFileName = m->getRootName(m->getSimpleName(sumFileName)) + toString(getpid()) + ".dist";
+                            ofstream outtemp;
+                            m->openOutputFile(tempdistFileName, outtemp);
+                            
+                            for (int i = 0; i < calcDists.size(); i++) {
+                                outtemp << calcDists[i].size() << endl;
+                                
+                                for (int j = 0; j < calcDists[i].size(); j++) {
+                                    outtemp << calcDists[i][j].seq1 << '\t' << calcDists[i][j].seq2 << '\t' << calcDists[i][j].dist << endl;
+                                }
+                            }
+                            outtemp.close();
+                        }
+                        
+                        exit(0);
+                    }else { 
+                        m->mothurOut("[ERROR]: unable to spawn the necessary processes."); m->mothurOutEndLine(); 
+                        for (int i = 0; i < processIDS.size(); i++) { kill (processIDS[i], SIGINT); }
+                        exit(0);
+                    }
+                }
+                
+                //parent do your part
+                driver(thisLookup, lines[0].start, lines[0].end, sumFileName + toString(getpid()) + ".temp", sumAllFileName + toString(getpid()) + ".temp", calcDists);   
+                m->appendFiles((sumFileName + toString(getpid()) + ".temp"), sumFileName);
+                m->mothurRemove((sumFileName + toString(getpid()) + ".temp"));
+                if (mult) { m->appendFiles((sumAllFileName + toString(getpid()) + ".temp"), sumAllFileName); }
+                
+                //force parent to wait until all the processes are done
+                for (int i = 0; i < processIDS.size(); i++) {
+                    int temp = processIDS[i];
+                    wait(&temp);
+                }
+                
+                for (int i = 0; i < processIDS.size(); i++) {
+                    m->appendFiles((sumFileName + toString(processIDS[i]) + ".temp"), sumFileName);
+                    m->mothurRemove((sumFileName + toString(processIDS[i]) + ".temp"));
+                    if (mult) {        m->mothurRemove((sumAllFileName + toString(processIDS[i]) + ".temp"));  }
+                    
+                    if (createPhylip) {
+                        string tempdistFileName = m->getRootName(m->getSimpleName(sumFileName)) + toString(processIDS[i]) +  ".dist";
+                        ifstream intemp;
+                        m->openInputFile(tempdistFileName, intemp);
+                        
+                        for (int k = 0; k < calcDists.size(); k++) {
+                            int size = 0;
+                            intemp >> size; m->gobble(intemp);
+                            
+                            for (int j = 0; j < size; j++) {
+                                int seq1 = 0;
+                                int seq2 = 0;
+                                float dist = 1.0;
+                                
+                                intemp >> seq1 >> seq2 >> dist;   m->gobble(intemp);
+                                
+                                seqDist tempDist(seq1, seq2, dist);
+                                calcDists[k].push_back(tempDist);
+                            }
+                        }
+                        intemp.close();
+                        m->mothurRemove(tempdistFileName);
+                    }
+                }
+#else
+                //////////////////////////////////////////////////////////////////////////////////////////////////////
+                //Windows version shared memory, so be careful when passing variables through the summarySharedData struct. 
+                //Above fork() will clone, so memory is separate, but that's not the case with windows, 
+                //Taking advantage of shared memory to pass results vectors.
+                //////////////////////////////////////////////////////////////////////////////////////////////////////
+                
+                vector<summarySharedData*> pDataArray; 
+                DWORD   dwThreadIdArray[processors-1];
+                HANDLE  hThreadArray[processors-1];
+                
+                //Create processor worker threads.
+                for( int i=1; i<processors; i++ ){
+                    
+                    //make copy of lookup so we don't get access violations
+                    vector<SharedRAbundVector*> newLookup;
+                    for (int k = 0; k < thisLookup.size(); k++) {
+                        SharedRAbundVector* temp = new SharedRAbundVector();
+                        temp->setLabel(thisLookup[k]->getLabel());
+                        temp->setGroup(thisLookup[k]->getGroup());
+                        newLookup.push_back(temp);
+                    }
+                
+                    
+                    //for each bin
+                    for (int k = 0; k < thisLookup[0]->getNumBins(); k++) {
+                        if (m->control_pressed) { for (int j = 0; j < newLookup.size(); j++) {  delete newLookup[j];  } return 0; }
+                        for (int j = 0; j < thisLookup.size(); j++) { newLookup[j]->push_back(thisLookup[j]->getAbundance(k), thisLookup[j]->getGroup()); }
+                    }
+                    
+                    // Allocate memory for thread data.
+                    summarySharedData* tempSum = new summarySharedData((sumFileName+toString(i)+".temp"), m, lines[i].start, lines[i].end, Estimators, newLookup);
+                    pDataArray.push_back(tempSum);
+                    processIDS.push_back(i);
+                    
+                    hThreadArray[i-1] = CreateThread(NULL, 0, MySummarySharedThreadFunction, pDataArray[i-1], 0, &dwThreadIdArray[i-1]);   
+                }
+                
+                //parent do your part
+                driver(thisLookup, lines[0].start, lines[0].end, sumFileName +"0.temp", sumAllFileName + "0.temp", calcDists);   
+                m->appendFiles((sumFileName + "0.temp"), sumFileName);
+                m->mothurRemove((sumFileName + "0.temp"));
+                if (mult) { m->appendFiles((sumAllFileName + "0.temp"), sumAllFileName); }
+                
+                //Wait until all threads have terminated.
+                WaitForMultipleObjects(processors-1, hThreadArray, TRUE, INFINITE);
+                
+                //Close all thread handles and free memory allocations.
+                for(int i=0; i < pDataArray.size(); i++){
+                    if (pDataArray[i]->count != (pDataArray[i]->end-pDataArray[i]->start)) {
+                        m->mothurOut("[ERROR]: process " + toString(i) + " only processed " + toString(pDataArray[i]->count) + " of " + toString(pDataArray[i]->end-pDataArray[i]->start) + " groups assigned to it, quitting. \n"); m->control_pressed = true; 
+                    }
+                    m->appendFiles((sumFileName + toString(processIDS[i]) + ".temp"), sumFileName);
+                    m->mothurRemove((sumFileName + toString(processIDS[i]) + ".temp"));
+                    
+                    for (int j = 0; j < pDataArray[i]->thisLookup.size(); j++) {  delete pDataArray[i]->thisLookup[j];  } 
+                    
+                    if (createPhylip) {
+                        for (int k = 0; k < calcDists.size(); k++) {
+                            int size = pDataArray[i]->calcDists[k].size();
+                            for (int j = 0; j < size; j++) {    calcDists[k].push_back(pDataArray[i]->calcDists[k][j]);    }
+                        }
+                    }
+                    
+                    CloseHandle(hThreadArray[i]);
+                    delete pDataArray[i];
+                }
+                
+#endif
+            }
+            
+            if (subsample && (thisIter != 0)) { //we want the summary results for the whole dataset, then the subsampling
+                
+                calcDistsTotals.push_back(calcDists); 
+                //clean up memory
+                for (int i = 0; i < thisItersLookup.size(); i++) { delete thisItersLookup[i]; }
+                thisItersLookup.clear();
+            }else {
+                if (createPhylip) {
+                    for (int i = 0; i < calcDists.size(); i++) {
+                        if (m->control_pressed) { break; }
+                        
+                        //initialize matrix
+                        vector< vector<double> > matrix; //square matrix to represent the distance
+                        matrix.resize(thisLookup.size());
+                        for (int k = 0; k < thisLookup.size(); k++) {  matrix[k].resize(thisLookup.size(), 0.0); }
+                        
+                        for (int j = 0; j < calcDists[i].size(); j++) {
+                            int row = calcDists[i][j].seq1;
+                            int column = calcDists[i][j].seq2;
+                            double dist = calcDists[i][j].dist;
+                            
+                            matrix[row][column] = dist;
+                            matrix[column][row] = dist;
+                        }
+                        
+                        map<string, string> variables; 
+                        variables["[filename]"] = outputDir + m->getRootName(m->getSimpleName(sharedfile));
+                        variables["[calc]"] = sumCalculators[i]->getName();
+                        variables["[distance]"] = thisLookup[0]->getLabel();
+                        variables["[outputtag]"] = output;
+                        variables["[tag2]"] = "";
+                        string distFileName = getOutputFileName("phylip",variables);
+                        outputNames.push_back(distFileName); outputTypes["phylip"].push_back(distFileName);
+                        ofstream outDist;
+                        m->openOutputFile(distFileName, outDist);
+                        outDist.setf(ios::fixed, ios::floatfield); outDist.setf(ios::showpoint);
+                        
+                        printSims(outDist, matrix);
+                        
+                        outDist.close();
+                    }
+                }
+            }
+            for (int i = 0; i < calcDists.size(); i++) {  calcDists[i].clear(); }
+               }
+
+        if (iters != 0) {
+            //we need to find the average distance and standard deviation for each groups distance
+            vector< vector<seqDist>  > calcAverages = m->getAverages(calcDistsTotals);
+            
+            //find standard deviation
+            vector< vector<seqDist>  > stdDev = m->getStandardDeviation(calcDistsTotals, calcAverages); 
+            
+            //print results
+            for (int i = 0; i < calcDists.size(); i++) {
+                vector< vector<double> > matrix; //square matrix to represent the distance
+                matrix.resize(thisLookup.size());
+                for (int k = 0; k < thisLookup.size(); k++) {  matrix[k].resize(thisLookup.size(), 0.0); }
+                
+                vector< vector<double> > stdmatrix; //square matrix to represent the stdDev
+                stdmatrix.resize(thisLookup.size());
+                for (int k = 0; k < thisLookup.size(); k++) {  stdmatrix[k].resize(thisLookup.size(), 0.0); }
+                
+                
+                for (int j = 0; j < calcAverages[i].size(); j++) {
+                    int row = calcAverages[i][j].seq1;
+                    int column = calcAverages[i][j].seq2;
+                    float dist = calcAverages[i][j].dist;
+                    float stdDist = stdDev[i][j].dist;
+                    
+                    matrix[row][column] = dist;
+                    matrix[column][row] = dist;
+                    stdmatrix[row][column] = stdDist;
+                    stdmatrix[column][row] = stdDist;
+                }
+                
+                map<string, string> variables; 
+                variables["[filename]"] = outputDir + m->getRootName(m->getSimpleName(sharedfile));
+                variables["[calc]"] = sumCalculators[i]->getName();
+                variables["[distance]"] = thisLookup[0]->getLabel();
+                variables["[outputtag]"] = output;
+                variables["[tag2]"] = "ave";
+                string distFileName = getOutputFileName("phylip",variables);
+                outputNames.push_back(distFileName); outputTypes["phylip"].push_back(distFileName);
+                ofstream outAve;
+                m->openOutputFile(distFileName, outAve);
+                outAve.setf(ios::fixed, ios::floatfield); outAve.setf(ios::showpoint);
+                
+                printSims(outAve, matrix);
+                
+                outAve.close();
+                
+                variables["[tag2]"] = "std";
+                distFileName = getOutputFileName("phylip",variables);
+                outputNames.push_back(distFileName); outputTypes["phylip"].push_back(distFileName);
+                ofstream outSTD;
+                m->openOutputFile(distFileName, outSTD);
+                outSTD.setf(ios::fixed, ios::floatfield); outSTD.setf(ios::showpoint);
+                
+                printSims(outSTD, stdmatrix);
+                
+                outSTD.close();
+                
+            }
+        }
+        
+        return 0;
        }
        catch(exception& e) {
                m->errorOut(e, "SummarySharedCommand", "process");