]> git.donarmstrong.com Git - mothur.git/blobdiff - seqsummarycommand.cpp
working on pam
[mothur.git] / seqsummarycommand.cpp
index 5776b970e6475bf74252d357ff1fef028902a797..70aa55d38502237388c520aeae892daf4891e73c 100644 (file)
@@ -8,53 +8,71 @@
  */
 
 #include "seqsummarycommand.h"
-#include "sequence.hpp"
+#include "counttable.h"
 
 //**********************************************************************************************************************
-vector<string> SeqSummaryCommand::getValidParameters(){        
+vector<string> SeqSummaryCommand::setParameters(){     
        try {
-               string Array[] =  {"fasta","processors","outputdir","inputdir"};
-               vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
+               CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "none", "none","summary",false,true,true); parameters.push_back(pfasta);
+               CommandParameter pname("name", "InputTypes", "", "", "namecount", "none", "none","",false,false,true); parameters.push_back(pname);
+        CommandParameter pcount("count", "InputTypes", "", "", "namecount", "none", "none","",false,false,true); parameters.push_back(pcount);
+               CommandParameter pprocessors("processors", "Number", "", "1", "", "", "","",false,false,true); parameters.push_back(pprocessors);
+               CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
+               CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
+               
+               vector<string> myArray;
+               for (int i = 0; i < parameters.size(); i++) {   myArray.push_back(parameters[i].name);          }
                return myArray;
        }
        catch(exception& e) {
-               m->errorOut(e, "SeqSummaryCommand", "getValidParameters");
+               m->errorOut(e, "SeqSummaryCommand", "setParameters");
                exit(1);
        }
 }
 //**********************************************************************************************************************
-SeqSummaryCommand::SeqSummaryCommand(){        
+string SeqSummaryCommand::getHelpString(){     
        try {
-               abort = true;
-               //initialize outputTypes
-               vector<string> tempOutNames;
-               outputTypes["summary"] = tempOutNames;
+               string helpString = "";
+               helpString += "The summary.seqs command reads a fastafile and summarizes the sequences.\n";
+               helpString += "The summary.seqs command parameters are fasta, name, count and processors, fasta is required, unless you have a valid current fasta file.\n";
+               helpString += "The name parameter allows you to enter a name file associated with your fasta file. \n";
+        helpString += "The count parameter allows you to enter a count file associated with your fasta file. \n";
+               helpString += "The summary.seqs command should be in the following format: \n";
+               helpString += "summary.seqs(fasta=yourFastaFile, processors=2) \n";
+               helpString += "Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFastaFile).\n";       
+               return helpString;
        }
        catch(exception& e) {
-               m->errorOut(e, "SeqSummaryCommand", "SeqSummaryCommand");
+               m->errorOut(e, "SeqSummaryCommand", "getHelpString");
                exit(1);
        }
 }
 //**********************************************************************************************************************
-vector<string> SeqSummaryCommand::getRequiredParameters(){     
-       try {
-               string Array[] =  {"fasta"};
-               vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
-               return myArray;
-       }
-       catch(exception& e) {
-               m->errorOut(e, "SeqSummaryCommand", "getRequiredParameters");
-               exit(1);
-       }
+string SeqSummaryCommand::getOutputPattern(string type) {
+    try {
+        string pattern = "";
+        
+        if (type == "summary") {  pattern = "[filename],summary"; } 
+        else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->control_pressed = true;  }
+        
+        return pattern;
+    }
+    catch(exception& e) {
+        m->errorOut(e, "SeqSummaryCommand", "getOutputPattern");
+        exit(1);
+    }
 }
+
 //**********************************************************************************************************************
-vector<string> SeqSummaryCommand::getRequiredFiles(){  
+SeqSummaryCommand::SeqSummaryCommand(){        
        try {
-               vector<string> myArray;
-               return myArray;
+               abort = true; calledHelp = true; 
+               setParameters();
+               vector<string> tempOutNames;
+               outputTypes["summary"] = tempOutNames;
        }
        catch(exception& e) {
-               m->errorOut(e, "SeqSummaryCommand", "getRequiredFiles");
+               m->errorOut(e, "SeqSummaryCommand", "SeqSummaryCommand");
                exit(1);
        }
 }
@@ -62,15 +80,14 @@ vector<string> SeqSummaryCommand::getRequiredFiles(){
 
 SeqSummaryCommand::SeqSummaryCommand(string option)  {
        try {
-               abort = false;
+               abort = false; calledHelp = false;   
                
                //allow user to run help
-               if(option == "help") { help(); abort = true; }
+               if(option == "help") { help(); abort = true; calledHelp = true; }
+               else if(option == "citation") { citation(); abort = true; calledHelp = true;}
                
                else {
-                       //valid paramters for this command
-                       string Array[] =  {"fasta","processors","outputdir","inputdir"};
-                       vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
+                       vector<string> myArray = setParameters();
                        
                        OptionParser parser(option);
                        map<string,string> parameters = parser.getParameters();
@@ -95,6 +112,22 @@ SeqSummaryCommand::SeqSummaryCommand(string option)  {
                                        //if the user has not given a path then, add inputdir. else leave path alone.
                                        if (path == "") {       parameters["fasta"] = inputDir + it->second;            }
                                }
+                               
+                               it = parameters.find("name");
+                               //user has given a template file
+                               if(it != parameters.end()){ 
+                                       path = m->hasPath(it->second);
+                                       //if the user has not given a path then, add inputdir. else leave path alone.
+                                       if (path == "") {       parameters["name"] = inputDir + it->second;             }
+                               }
+                
+                it = parameters.find("count");
+                               //user has given a template file
+                               if(it != parameters.end()){ 
+                                       path = m->hasPath(it->second);
+                                       //if the user has not given a path then, add inputdir. else leave path alone.
+                                       if (path == "") {       parameters["count"] = inputDir + it->second;            }
+                               }
                        }
                        
                        //initialize outputTypes
@@ -104,7 +137,23 @@ SeqSummaryCommand::SeqSummaryCommand(string option)  {
                        //check for required parameters
                        fastafile = validParameter.validFile(parameters, "fasta", true);
                        if (fastafile == "not open") { abort = true; }
-                       else if (fastafile == "not found") { fastafile = ""; m->mothurOut("fasta is a required parameter for the summary.seqs command."); m->mothurOutEndLine(); abort = true;  }       
+                       else if (fastafile == "not found") {                            
+                               fastafile = m->getFastaFile(); 
+                               if (fastafile != "") { m->mothurOut("Using " + fastafile + " as input file for the fasta parameter."); m->mothurOutEndLine(); }
+                               else {  m->mothurOut("You have no current fastafile and the fasta parameter is required."); m->mothurOutEndLine(); abort = true; }
+                       }else { m->setFastaFile(fastafile); }   
+                       
+                       namefile = validParameter.validFile(parameters, "name", true);
+                       if (namefile == "not open") { namefile = ""; abort = true; }
+                       else if (namefile == "not found") { namefile = "";  }   
+                       else { m->setNameFile(namefile); }
+            
+            countfile = validParameter.validFile(parameters, "count", true);
+                       if (countfile == "not open") { abort = true; countfile = ""; }  
+                       else if (countfile == "not found") { countfile = ""; }
+                       else { m->setCountTableFile(countfile); }
+                       
+            if ((countfile != "") && (namefile != "")) { m->mothurOut("You must enter ONLY ONE of the following: count or name."); m->mothurOutEndLine(); abort = true; }
                        
                        //if the user changes the output directory command factory will send this info to us in the output parameter 
                        outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){  
@@ -112,10 +161,16 @@ SeqSummaryCommand::SeqSummaryCommand(string option)  {
                                outputDir += m->hasPath(fastafile); //if user entered a file with a path then preserve it       
                        }
                        
-                       string temp = validParameter.validFile(parameters, "processors", false);        if (temp == "not found"){       temp = "1";                             }
-                       convert(temp, processors); 
-
-
+                       string temp = validParameter.validFile(parameters, "processors", false);        if (temp == "not found"){       temp = m->getProcessors();      }
+                       m->setProcessors(temp);
+                       m->mothurConvert(temp, processors);
+                       
+            if (countfile == "") {
+                if (namefile == "") {
+                    vector<string> files; files.push_back(fastafile);
+                    parser.getNameFile(files);
+                }
+            }
                }
        }
        catch(exception& e) {
@@ -123,34 +178,19 @@ SeqSummaryCommand::SeqSummaryCommand(string option)  {
                exit(1);
        }
 }
-//**********************************************************************************************************************
-
-void SeqSummaryCommand::help(){
-       try {
-               m->mothurOut("The summary.seqs command reads a fastafile and summarizes the sequences.\n");
-               m->mothurOut("The summary.seqs command parameters are fasta and processors, fasta is required.\n");
-               m->mothurOut("The summary.seqs command should be in the following format: \n");
-               m->mothurOut("summary.seqs(fasta=yourFastaFile, processors=2) \n");
-               m->mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFastaFile).\n\n");     
-       }
-       catch(exception& e) {
-               m->errorOut(e, "SeqSummaryCommand", "help");
-               exit(1);
-       }
-}
-
-//***************************************************************************************************************
-
-SeqSummaryCommand::~SeqSummaryCommand(){       /*      do nothing      */      }
-
 //***************************************************************************************************************
 
 int SeqSummaryCommand::execute(){
        try{
                
-               if (abort == true) { return 0; }
+               if (abort == true) { if (calledHelp) { return 0; }  return 2;   }
                
-               string summaryFile = outputDir + m->getSimpleName(fastafile) + ".summary";
+               //set current fasta to fastafile
+               m->setFastaFile(fastafile);
+               
+        map<string, string> variables; 
+               variables["[filename]"] = outputDir + m->getRootName(m->getSimpleName(fastafile));
+               string summaryFile = getOutputFileName("summary",variables);
                                
                int numSeqs = 0;
                
@@ -159,13 +199,22 @@ int SeqSummaryCommand::execute(){
                vector<int> seqLength;
                vector<int> ambigBases;
                vector<int> longHomoPolymer;
+               
+               if (namefile != "") { nameMap = m->readNames(namefile); }
+        else if (countfile != "") {
+            CountTable ct;
+            ct.readTable(countfile, false, false);
+            nameMap = ct.getNameMap();
+        }
+               
+               if (m->control_pressed) { return 0; }
                        
 #ifdef USE_MPI 
                                int pid, numSeqsPerProcessor; 
                                int tag = 2001;
                                int startTag = 1; int endTag = 2; int lengthTag = 3; int baseTag = 4; int lhomoTag = 5;
                                int outMode=MPI_MODE_CREATE|MPI_MODE_WRONLY; 
-                               vector<unsigned long int> MPIPos;
+                               vector<unsigned long long> MPIPos;
                                
                                MPI_Status status; 
                                MPI_Status statusOut;
@@ -187,7 +236,7 @@ int SeqSummaryCommand::execute(){
                                
                                if (pid == 0) { //you are the root process
                                                //print header
-                                               string outputString = "seqname\tstart\tend\tnbases\tambigs\tpolymer\n"; 
+                                               string outputString = "seqname\tstart\tend\tnbases\tambigs\tpolymer\tnumSeqs\n";        
                                                int length = outputString.length();
                                                char* buf2 = new char[length];
                                                memcpy(buf2, outputString.c_str(), length);
@@ -276,31 +325,31 @@ int SeqSummaryCommand::execute(){
                                
                                MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case
 #else
-                       vector<unsigned long int> positions = m->divideFile(fastafile, processors);
-                               
-                       for (int i = 0; i < (positions.size()-1); i++) {
-                               lines.push_back(new linePair(positions[i], positions[(i+1)]));
-                       }       
-
-               #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
-                               if(processors == 1){
-                                       numSeqs = driverCreateSummary(startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, fastafile, summaryFile, lines[0]);
-                               }else{
-                                       numSeqs = createProcessesCreateSummary(startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, fastafile, summaryFile); 
-                                       
-                                       rename((summaryFile + toString(processIDS[0]) + ".temp").c_str(), summaryFile.c_str());
-                                       //append files
-                                       for(int i=1;i<processors;i++){
-                                               m->appendFiles((summaryFile + toString(processIDS[i]) + ".temp"), summaryFile);
-                                               remove((summaryFile + toString(processIDS[i]) + ".temp").c_str());
-                                       }
+                       vector<unsigned long long> positions; 
+                       #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
+                               positions = m->divideFile(fastafile, processors);
+                               for (int i = 0; i < (positions.size()-1); i++) {        lines.push_back(new linePair(positions[i], positions[(i+1)]));  }
+                       #else
+                               positions = m->setFilePosFasta(fastafile, numSeqs); 
+                if (positions.size() < processors) { processors = positions.size(); }
+               
+                               //figure out how many sequences you have to process
+                               int numSeqsPerProcessor = numSeqs / processors;
+                               for (int i = 0; i < processors; i++) {
+                                       int startIndex =  i * numSeqsPerProcessor;
+                                       if(i == (processors - 1)){      numSeqsPerProcessor = numSeqs - i * numSeqsPerProcessor;        }
+                                       lines.push_back(new linePair(positions[startIndex], numSeqsPerProcessor));
                                }
-                               
-                               if (m->control_pressed) {  return 0; }
-               #else
+                       #endif
+                       
+
+                       if(processors == 1){
                                numSeqs = driverCreateSummary(startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, fastafile, summaryFile, lines[0]);
-                               if (m->control_pressed) {  return 0; }
-               #endif
+                       }else{
+                               numSeqs = createProcessesCreateSummary(startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, fastafile, summaryFile); 
+                       }
+                       
+                       if (m->control_pressed) {  return 0; }
 #endif
                        
                #ifdef USE_MPI
@@ -312,35 +361,54 @@ int SeqSummaryCommand::execute(){
                sort(seqLength.begin(), seqLength.end());
                sort(ambigBases.begin(), ambigBases.end());
                sort(longHomoPolymer.begin(), longHomoPolymer.end());
+               int size = startPosition.size();
                
-               int ptile0_25   = int(numSeqs * 0.025);
-               int ptile25             = int(numSeqs * 0.250);
-               int ptile50             = int(numSeqs * 0.500);
-               int ptile75             = int(numSeqs * 0.750);
-               int ptile97_5   = int(numSeqs * 0.975);
-               int ptile100    = numSeqs - 1;
+               //find means
+               unsigned long long meanStartPosition, meanEndPosition, meanSeqLength, meanAmbigBases, meanLongHomoPolymer;
+               meanStartPosition = 0; meanEndPosition = 0; meanSeqLength = 0; meanAmbigBases = 0; meanLongHomoPolymer = 0;
+               for (int i = 0; i < size; i++) {
+                       meanStartPosition += startPosition[i];
+                       meanEndPosition += endPosition[i];
+                       meanSeqLength += seqLength[i];
+                       meanAmbigBases += ambigBases[i];
+                       meanLongHomoPolymer += longHomoPolymer[i];
+               }
+                
+        double meanstartPosition, meanendPosition, meanseqLength, meanambigBases, meanlongHomoPolymer;
+                
+               meanstartPosition = meanStartPosition / (double) size; meanendPosition = meanEndPosition /(double) size; meanlongHomoPolymer = meanLongHomoPolymer / (double) size; meanseqLength = meanSeqLength / (double) size; meanambigBases = meanAmbigBases /(double) size;
+                               
+               int ptile0_25   = int(size * 0.025);
+               int ptile25             = int(size * 0.250);
+               int ptile50             = int(size * 0.500);
+               int ptile75             = int(size * 0.750);
+               int ptile97_5   = int(size * 0.975);
+               int ptile100    = size - 1;
                
                //to compensate for blank sequences that would result in startPosition and endPostion equalling -1
                if (startPosition[0] == -1) {  startPosition[0] = 0;    }
                if (endPosition[0] == -1)       {  endPosition[0] = 0;          }
                
-               if (m->control_pressed) {  remove(summaryFile.c_str()); return 0; }
+               if (m->control_pressed) {  m->mothurRemove(summaryFile); return 0; }
                
                m->mothurOutEndLine();
-               m->mothurOut("\t\tStart\tEnd\tNBases\tAmbigs\tPolymer"); m->mothurOutEndLine();
-               m->mothurOut("Minimum:\t" + toString(startPosition[0]) + "\t" + toString(endPosition[0]) + "\t" + toString(seqLength[0]) + "\t" + toString(ambigBases[0]) + "\t" + toString(longHomoPolymer[0])); m->mothurOutEndLine();
-               m->mothurOut("2.5%-tile:\t" + toString(startPosition[ptile0_25]) + "\t" + toString(endPosition[ptile0_25]) + "\t" + toString(seqLength[ptile0_25]) + "\t" + toString(ambigBases[ptile0_25]) + "\t"+ toString(longHomoPolymer[ptile0_25])); m->mothurOutEndLine();
-               m->mothurOut("25%-tile:\t" + toString(startPosition[ptile25]) + "\t" + toString(endPosition[ptile25]) + "\t" + toString(seqLength[ptile25]) + "\t" + toString(ambigBases[ptile25]) + "\t" + toString(longHomoPolymer[ptile25])); m->mothurOutEndLine();
-               m->mothurOut("Median: \t" + toString(startPosition[ptile50]) + "\t" + toString(endPosition[ptile50]) + "\t" + toString(seqLength[ptile50]) + "\t" + toString(ambigBases[ptile50]) + "\t" + toString(longHomoPolymer[ptile50])); m->mothurOutEndLine();
-               m->mothurOut("75%-tile:\t" + toString(startPosition[ptile75]) + "\t" + toString(endPosition[ptile75]) + "\t" + toString(seqLength[ptile75]) + "\t" + toString(ambigBases[ptile75]) + "\t" + toString(longHomoPolymer[ptile75])); m->mothurOutEndLine();
-               m->mothurOut("97.5%-tile:\t" + toString(startPosition[ptile97_5]) + "\t" + toString(endPosition[ptile97_5]) + "\t" + toString(seqLength[ptile97_5]) + "\t" + toString(ambigBases[ptile97_5]) + "\t" + toString(longHomoPolymer[ptile97_5])); m->mothurOutEndLine();
-               m->mothurOut("Maximum:\t" + toString(startPosition[ptile100]) + "\t" + toString(endPosition[ptile100]) + "\t" + toString(seqLength[ptile100]) + "\t" + toString(ambigBases[ptile100]) + "\t" + toString(longHomoPolymer[ptile100])); m->mothurOutEndLine();
-               m->mothurOut("# of Seqs:\t" + toString(numSeqs)); m->mothurOutEndLine();
-               
-               if (m->control_pressed) {  remove(summaryFile.c_str()); return 0; }
+               m->mothurOut("\t\tStart\tEnd\tNBases\tAmbigs\tPolymer\tNumSeqs"); m->mothurOutEndLine();
+               m->mothurOut("Minimum:\t" + toString(startPosition[0]) + "\t" + toString(endPosition[0]) + "\t" + toString(seqLength[0]) + "\t" + toString(ambigBases[0]) + "\t" + toString(longHomoPolymer[0]) + "\t" + toString(1)); m->mothurOutEndLine();
+               m->mothurOut("2.5%-tile:\t" + toString(startPosition[ptile0_25]) + "\t" + toString(endPosition[ptile0_25]) + "\t" + toString(seqLength[ptile0_25]) + "\t" + toString(ambigBases[ptile0_25]) + "\t"+ toString(longHomoPolymer[ptile0_25]) + "\t" + toString(ptile0_25+1)); m->mothurOutEndLine();
+               m->mothurOut("25%-tile:\t" + toString(startPosition[ptile25]) + "\t" + toString(endPosition[ptile25]) + "\t" + toString(seqLength[ptile25]) + "\t" + toString(ambigBases[ptile25]) + "\t" + toString(longHomoPolymer[ptile25]) + "\t" + toString(ptile25+1)); m->mothurOutEndLine();
+               m->mothurOut("Median: \t" + toString(startPosition[ptile50]) + "\t" + toString(endPosition[ptile50]) + "\t" + toString(seqLength[ptile50]) + "\t" + toString(ambigBases[ptile50]) + "\t" + toString(longHomoPolymer[ptile50]) + "\t" + toString(ptile50+1)); m->mothurOutEndLine();
+               m->mothurOut("75%-tile:\t" + toString(startPosition[ptile75]) + "\t" + toString(endPosition[ptile75]) + "\t" + toString(seqLength[ptile75]) + "\t" + toString(ambigBases[ptile75]) + "\t" + toString(longHomoPolymer[ptile75]) + "\t" + toString(ptile75+1)); m->mothurOutEndLine();
+               m->mothurOut("97.5%-tile:\t" + toString(startPosition[ptile97_5]) + "\t" + toString(endPosition[ptile97_5]) + "\t" + toString(seqLength[ptile97_5]) + "\t" + toString(ambigBases[ptile97_5]) + "\t" + toString(longHomoPolymer[ptile97_5]) + "\t" + toString(ptile97_5+1)); m->mothurOutEndLine();
+               m->mothurOut("Maximum:\t" + toString(startPosition[ptile100]) + "\t" + toString(endPosition[ptile100]) + "\t" + toString(seqLength[ptile100]) + "\t" + toString(ambigBases[ptile100]) + "\t" + toString(longHomoPolymer[ptile100]) + "\t" + toString(ptile100+1)); m->mothurOutEndLine();
+               m->mothurOut("Mean:\t" + toString(meanstartPosition) + "\t" + toString(meanendPosition) + "\t" + toString(meanseqLength) + "\t" + toString(meanambigBases) + "\t" + toString(meanlongHomoPolymer)); m->mothurOutEndLine();
+
+               if ((namefile == "") && (countfile == "")) {  m->mothurOut("# of Seqs:\t" + toString(numSeqs)); m->mothurOutEndLine(); }
+               else { m->mothurOut("# of unique seqs:\t" + toString(numSeqs)); m->mothurOutEndLine(); m->mothurOut("total # of seqs:\t" + toString(startPosition.size())); m->mothurOutEndLine(); }
+               
+               if (m->control_pressed) {  m->mothurRemove(summaryFile); return 0; }
                
                m->mothurOutEndLine();
-               m->mothurOut("Output File Name: "); m->mothurOutEndLine();
+               m->mothurOut("Output File Names: "); m->mothurOutEndLine();
                m->mothurOut(summaryFile); m->mothurOutEndLine();       outputNames.push_back(summaryFile); outputTypes["summary"].push_back(summaryFile);
                m->mothurOutEndLine();
                
@@ -348,6 +416,13 @@ int SeqSummaryCommand::execute(){
                        }
                #endif
 
+        //set fasta file as new current fastafile
+               string current = "";
+               itTypes = outputTypes.find("summary");
+               if (itTypes != outputTypes.end()) {
+                       if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setSummaryFile(current); }
+               }
+        
                return 0;
        }
        catch(exception& e) {
@@ -364,7 +439,7 @@ int SeqSummaryCommand::driverCreateSummary(vector<int>& startPosition, vector<in
                
                //print header if you are process 0
                if (filePos->start == 0) {
-                       outSummary << "seqname\tstart\tend\tnbases\tambigs\tpolymer" << endl;   
+                       outSummary << "seqname\tstart\tend\tnbases\tambigs\tpolymer\tnumSeqs" << endl;  
                }
                                
                ifstream in;
@@ -374,40 +449,54 @@ int SeqSummaryCommand::driverCreateSummary(vector<int>& startPosition, vector<in
 
                bool done = false;
                int count = 0;
-       
+        
+        
                while (!done) {
                                
                        if (m->control_pressed) { in.close(); outSummary.close(); return 1; }
-                                       
+            
+            if (m->debug) { m->mothurOut("[DEBUG]: count = " + toString(count) + "\n");  }
+            
                        Sequence current(in); m->gobble(in);
-       
+           
                        if (current.getName() != "") {
-                               startPosition.push_back(current.getStartPos());
-                               endPosition.push_back(current.getEndPos());
-                               seqLength.push_back(current.getNumBases());
-                               ambigBases.push_back(current.getAmbigBases());
-                               longHomoPolymer.push_back(current.getLongHomoPolymer());
                                
+                if (m->debug) { m->mothurOut("[DEBUG]: " + current.getName() + '\t' + toString(current.getNumBases()) + "\n");  }
+                
+                               int num = 1;
+                               if ((namefile != "") || (countfile != "")) {
+                                       //make sure this sequence is in the namefile, else error 
+                                       map<string, int>::iterator it = nameMap.find(current.getName());
+                                       
+                                       if (it == nameMap.end()) { m->mothurOut("[ERROR]: '" + current.getName() + "' is not in your name or count file, please correct."); m->mothurOutEndLine(); m->control_pressed = true; }
+                                       else { num = it->second; }
+                               }
+                               
+                               //for each sequence this sequence represents
+                               for (int i = 0; i < num; i++) {
+                                       startPosition.push_back(current.getStartPos());
+                                       endPosition.push_back(current.getEndPos());
+                                       seqLength.push_back(current.getNumBases());
+                                       ambigBases.push_back(current.getAmbigBases());
+                                       longHomoPolymer.push_back(current.getLongHomoPolymer());
+                               }
+                               count++;
                                outSummary << current.getName() << '\t';
                                outSummary << current.getStartPos() << '\t' << current.getEndPos() << '\t';
                                outSummary << current.getNumBases() << '\t' << current.getAmbigBases() << '\t';
-                               outSummary << current.getLongHomoPolymer() << endl;
-                               count++;
+                               outSummary << current.getLongHomoPolymer() << '\t' << num << endl;
+                
+                if (m->debug) { m->mothurOut("[DEBUG]: " + current.getName() + '\t' + toString(current.getNumBases()) + "\n");  }
                        }
                        
-                       #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
-                               unsigned long int pos = in.tellg();
+                       #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
+                               unsigned long long pos = in.tellg();
                                if ((pos == -1) || (pos >= filePos->end)) { break; }
                        #else
                                if (in.eof()) { break; }
                        #endif
-                       
-                       //report progress
-                       //if((count) % 100 == 0){       m->mothurOut(toString(count)); m->mothurOutEndLine();           }
                }
-               //report progress
-               //if((count) % 100 != 0){       m->mothurOut(toString(count)); m->mothurOutEndLine();           }
-               
+                               
                in.close();
                
                return count;
@@ -419,14 +508,13 @@ int SeqSummaryCommand::driverCreateSummary(vector<int>& startPosition, vector<in
 }
 #ifdef USE_MPI
 /**************************************************************************************/
-int SeqSummaryCommand::MPICreateSummary(int start, int num, vector<int>& startPosition, vector<int>& endPosition, vector<int>& seqLength, vector<int>& ambigBases, vector<int>& longHomoPolymer, MPI_File& inMPI, MPI_File& outMPI, vector<unsigned long int>& MPIPos) {       
+int SeqSummaryCommand::MPICreateSummary(int start, int num, vector<int>& startPosition, vector<int>& endPosition, vector<int>& seqLength, vector<int>& ambigBases, vector<int>& longHomoPolymer, MPI_File& inMPI, MPI_File& outMPI, vector<unsigned long long>& MPIPos) {      
        try {
                
                int pid;
                MPI_Status status; 
                MPI_Comm_rank(MPI_COMM_WORLD, &pid); 
 
-               
                for(int i=0;i<num;i++){
                        
                        if (m->control_pressed) { return 0; }
@@ -445,14 +533,27 @@ int SeqSummaryCommand::MPICreateSummary(int start, int num, vector<int>& startPo
                        Sequence current(iss);  
 
                        if (current.getName() != "") {
-                               startPosition.push_back(current.getStartPos());
-                               endPosition.push_back(current.getEndPos());
-                               seqLength.push_back(current.getNumBases());
-                               ambigBases.push_back(current.getAmbigBases());
-                               longHomoPolymer.push_back(current.getLongHomoPolymer());
+                               
+                               int num = 1;
+                               if ((namefile != "") || (countfile != "")) {
+                                       //make sure this sequence is in the namefile, else error 
+                                       map<string, int>::iterator it = nameMap.find(current.getName());
+                                       
+                                       if (it == nameMap.end()) { cout << "[ERROR]: " << current.getName() << " is not in your name or count file, please correct." << endl; m->control_pressed = true; }
+                                       else { num = it->second; }
+                               }
+                               
+                               //for each sequence this sequence represents
+                               for (int j = 0; j < num; j++) {
+                                       startPosition.push_back(current.getStartPos());
+                                       endPosition.push_back(current.getEndPos());
+                                       seqLength.push_back(current.getNumBases());
+                                       ambigBases.push_back(current.getAmbigBases());
+                                       longHomoPolymer.push_back(current.getLongHomoPolymer());
+                               }
                                
                                string outputString = current.getName() + "\t" + toString(current.getStartPos()) + "\t" + toString(current.getEndPos()) + "\t";
-                               outputString += toString(current.getNumBases()) + "\t" + toString(current.getAmbigBases()) + "\t" + toString(current.getLongHomoPolymer()) + "\n";
+                               outputString += toString(current.getNumBases()) + "\t" + toString(current.getAmbigBases()) + "\t" + toString(current.getLongHomoPolymer()) + "\t" + toString(num) + "\n";
                                
                                //output to file
                                length = outputString.length();
@@ -475,11 +576,12 @@ int SeqSummaryCommand::MPICreateSummary(int start, int num, vector<int>& startPo
 /**************************************************************************************************/
 int SeqSummaryCommand::createProcessesCreateSummary(vector<int>& startPosition, vector<int>& endPosition, vector<int>& seqLength, vector<int>& ambigBases, vector<int>& longHomoPolymer, string filename, string sumFile) {
        try {
-#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
-               int process = 0;
+               int process = 1;
                int num = 0;
                processIDS.clear();
                
+#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
+               
                //loop through and create all the processes you want
                while (process != processors) {
                        int pid = fork();
@@ -496,6 +598,7 @@ int SeqSummaryCommand::createProcessesCreateSummary(vector<int>& startPosition,
                                m->openOutputFile(tempFile, out);
                                
                                out << num << endl;
+                               out << startPosition.size() << endl;
                                for (int k = 0; k < startPosition.size(); k++)          {               out << startPosition[k] << '\t'; }  out << endl;
                                for (int k = 0; k < endPosition.size(); k++)            {               out << endPosition[k] << '\t'; }  out << endl;
                                for (int k = 0; k < seqLength.size(); k++)                      {               out << seqLength[k] << '\t'; }  out << endl;
@@ -505,11 +608,18 @@ int SeqSummaryCommand::createProcessesCreateSummary(vector<int>& startPosition,
                                out.close();
                                
                                exit(0);
-                       }else { m->mothurOut("unable to spawn the necessary processes."); m->mothurOutEndLine(); exit(0); }
+                       }else { 
+                               m->mothurOut("[ERROR]: unable to spawn the necessary processes."); m->mothurOutEndLine(); 
+                               for (int i = 0; i < processIDS.size(); i++) { kill (processIDS[i], SIGINT); }
+                               exit(0);
+                       }
                }
                
+               //do your part
+               num = driverCreateSummary(startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, fastafile, sumFile, lines[0]);
+
                //force parent to wait until all the processes are done
-               for (int i=0;i<processors;i++) { 
+               for (int i=0;i<processIDS.size();i++) { 
                        int temp = processIDS[i];
                        wait(&temp);
                }
@@ -522,6 +632,7 @@ int SeqSummaryCommand::createProcessesCreateSummary(vector<int>& startPosition,
                        
                        int temp, tempNum;
                        in >> tempNum; m->gobble(in); num += tempNum;
+                       in >> tempNum; m->gobble(in);
                        for (int k = 0; k < tempNum; k++)                       {               in >> temp; startPosition.push_back(temp);              }               m->gobble(in);
                        for (int k = 0; k < tempNum; k++)                       {               in >> temp; endPosition.push_back(temp);                }               m->gobble(in);
                        for (int k = 0; k < tempNum; k++)                       {               in >> temp; seqLength.push_back(temp);                  }               m->gobble(in);
@@ -529,17 +640,76 @@ int SeqSummaryCommand::createProcessesCreateSummary(vector<int>& startPosition,
                        for (int k = 0; k < tempNum; k++)                       {               in >> temp; longHomoPolymer.push_back(temp);    }               m->gobble(in);
                                
                        in.close();
-                       remove(tempFilename.c_str());
+                       m->mothurRemove(tempFilename);
+                       
+                       m->appendFiles((sumFile + toString(processIDS[i]) + ".temp"), sumFile);
+                       m->mothurRemove((sumFile + toString(processIDS[i]) + ".temp"));
                }
                
-               return num;
+#else
+               //////////////////////////////////////////////////////////////////////////////////////////////////////
+               //Windows version shared memory, so be careful when passing variables through the seqSumData struct. 
+               //Above fork() will clone, so memory is separate, but that's not the case with windows, 
+               //Taking advantage of shared memory to allow both threads to add info to vectors.
+               //////////////////////////////////////////////////////////////////////////////////////////////////////
+               
+               vector<seqSumData*> pDataArray; 
+               DWORD   dwThreadIdArray[processors-1];
+               HANDLE  hThreadArray[processors-1]; 
+        
+               bool hasNameMap = false;
+        if ((namefile !="") || (countfile != "")) { hasNameMap = true; }
+        
+               //Create processor worker threads.
+               for( int i=0; i<processors-1; i++ ){
+            
+            string extension = "";
+            if (i != 0) { extension = toString(i) + ".temp"; processIDS.push_back(i); }
+                       // Allocate memory for thread data.
+                       seqSumData* tempSum = new seqSumData(filename, (sumFile+extension), m, lines[i]->start, lines[i]->end, hasNameMap, nameMap);
+                       pDataArray.push_back(tempSum);
+                       
+                       //MySeqSumThreadFunction is in header. It must be global or static to work with the threads.
+                       //default security attributes, thread function name, argument to thread function, use default creation flags, returns the thread identifier
+                       hThreadArray[i] = CreateThread(NULL, 0, MySeqSumThreadFunction, pDataArray[i], 0, &dwThreadIdArray[i]);   
+               }
+               
+        //do your part
+               num = driverCreateSummary(startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, fastafile, (sumFile+toString(processors-1)+".temp"), lines[processors-1]);
+        processIDS.push_back(processors-1);
+
+               //Wait until all threads have terminated.
+               WaitForMultipleObjects(processors-1, hThreadArray, TRUE, INFINITE);
+               
+               //Close all thread handles and free memory allocations.
+               for(int i=0; i < pDataArray.size(); i++){
+                       num += pDataArray[i]->count;
+            if (pDataArray[i]->count != pDataArray[i]->end) {
+                m->mothurOut("[ERROR]: process " + toString(i) + " only processed " + toString(pDataArray[i]->count) + " of " + toString(pDataArray[i]->end) + " sequences assigned to it, quitting. \n"); m->control_pressed = true; 
+            }
+            for (int k = 0; k < pDataArray[i]->startPosition.size(); k++) {    startPosition.push_back(pDataArray[i]->startPosition[k]);       }
+                       for (int k = 0; k < pDataArray[i]->endPosition.size(); k++) {   endPosition.push_back(pDataArray[i]->endPosition[k]);       }
+            for (int k = 0; k < pDataArray[i]->seqLength.size(); k++) {        seqLength.push_back(pDataArray[i]->seqLength[k]);       }
+            for (int k = 0; k < pDataArray[i]->ambigBases.size(); k++) {       ambigBases.push_back(pDataArray[i]->ambigBases[k]);       }
+            for (int k = 0; k < pDataArray[i]->longHomoPolymer.size(); k++) {  longHomoPolymer.push_back(pDataArray[i]->longHomoPolymer[k]);       }
+                       CloseHandle(hThreadArray[i]);
+                       delete pDataArray[i];
+               }
+    
+               //append files
+               for(int i=0;i<processIDS.size();i++){
+                       m->appendFiles((sumFile + toString(processIDS[i]) + ".temp"), sumFile);
+                       m->mothurRemove((sumFile + toString(processIDS[i]) + ".temp"));
+               }
 #endif         
+               return num;
        }
        catch(exception& e) {
                m->errorOut(e, "SeqSummaryCommand", "createProcessesCreateSummary");
                exit(1);
        }
 }
-//***************************************************************************************************************
+/**********************************************************************************************************************/
+