CommandParameter pgroup("group", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(pgroup);
CommandParameter pqfile("qfile", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(pqfile);
CommandParameter palignreport("alignreport", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(palignreport);
+ CommandParameter ptax("taxonomy", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(ptax);
CommandParameter pstart("start", "Number", "", "-1", "", "", "",false,false); parameters.push_back(pstart);
CommandParameter pend("end", "Number", "", "-1", "", "", "",false,false); parameters.push_back(pend);
CommandParameter pmaxambig("maxambig", "Number", "", "-1", "", "", "",false,false); parameters.push_back(pmaxambig);
try {
string helpString = "";
helpString += "The screen.seqs command reads a fastafile and creates .....\n";
- helpString += "The screen.seqs command parameters are fasta, start, end, maxambig, maxhomop, minlength, maxlength, name, group, qfile, optimize, criteria and processors.\n";
+ helpString += "The screen.seqs command parameters are fasta, start, end, maxambig, maxhomop, minlength, maxlength, name, group, qfile, alignreport, taxonomy, optimize, criteria and processors.\n";
helpString += "The fasta parameter is required.\n";
+ helpString += "The alignreport and taxonomy parameters allow you to remove bad seqs from taxonomy and alignreport files.\n";
helpString += "The start parameter .... The default is -1.\n";
helpString += "The end parameter .... The default is -1.\n";
helpString += "The maxambig parameter allows you to set the maximum number of ambigious bases allowed. The default is -1.\n";
outputTypes["alignreport"] = tempOutNames;
outputTypes["accnos"] = tempOutNames;
outputTypes["qfile"] = tempOutNames;
+ outputTypes["taxonomy"] = tempOutNames;
}
catch(exception& e) {
m->errorOut(e, "ScreenSeqsCommand", "ScreenSeqsCommand");
//allow user to run help
if(option == "help") { help(); abort = true; calledHelp = true; }
+ else if(option == "citation") { citation(); abort = true; calledHelp = true;}
else {
vector<string> myArray = setParameters();
outputTypes["alignreport"] = tempOutNames;
outputTypes["accnos"] = tempOutNames;
outputTypes["qfile"] = tempOutNames;
+ outputTypes["taxonomy"] = tempOutNames;
//if the user changes the input directory command factory will send this info to us in the output parameter
string inputDir = validParameter.validFile(parameters, "inputdir", false);
if (path == "") { parameters["qfile"] = inputDir + it->second; }
}
+ it = parameters.find("taxonomy");
+ //user has given a template file
+ if(it != parameters.end()){
+ path = m->hasPath(it->second);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { parameters["taxonomy"] = inputDir + it->second; }
+ }
}
//check for required parameters
if (fastafile != "") { m->mothurOut("Using " + fastafile + " as input file for the fasta parameter."); m->mothurOutEndLine(); }
else { m->mothurOut("You have no current fastafile and the fasta parameter is required."); m->mothurOutEndLine(); abort = true; }
}
- else if (fastafile == "not open") { abort = true; }
+ else if (fastafile == "not open") { abort = true; }
+ else { m->setFastaFile(fastafile); }
groupfile = validParameter.validFile(parameters, "group", true);
if (groupfile == "not open") { abort = true; }
else if (groupfile == "not found") { groupfile = ""; }
+ else { m->setGroupFile(groupfile); }
qualfile = validParameter.validFile(parameters, "qfile", true);
if (qualfile == "not open") { abort = true; }
else if (qualfile == "not found") { qualfile = ""; }
+ else { m->setQualFile(qualfile); }
namefile = validParameter.validFile(parameters, "name", true);
if (namefile == "not open") { namefile = ""; abort = true; }
else if (namefile == "not found") { namefile = ""; }
-
+ else { m->setNameFile(namefile); }
+
alignreport = validParameter.validFile(parameters, "alignreport", true);
if (alignreport == "not open") { abort = true; }
- else if (alignreport == "not found") { alignreport = ""; }
+ else if (alignreport == "not found") { alignreport = ""; }
+
+ taxonomy = validParameter.validFile(parameters, "taxonomy", true);
+ if (taxonomy == "not open") { abort = true; }
+ else if (taxonomy == "not found") { taxonomy = ""; }
//if the user changes the output directory command factory will send this info to us in the output parameter
outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){
if (abort == true) { if (calledHelp) { return 0; } return 2; }
//if the user want to optimize we need to know the 90% mark
- vector<unsigned long int> positions;
+ vector<unsigned long long> positions;
if (optimize.size() != 0) { //get summary is paralellized so we need to divideFile, no need to do this step twice so I moved it here
//use the namefile to optimize correctly
if (namefile != "") { nameMap = m->readNames(namefile); }
#ifdef USE_MPI
int pid, numSeqsPerProcessor;
int tag = 2001;
- vector<unsigned long int> MPIPos;
+ vector<unsigned long long> MPIPos;
MPI_Status status;
MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
if(processors == 1){
numFastaSeqs = driver(lines[0], goodSeqFile, badAccnosFile, fastafile, badSeqNames);
- if (m->control_pressed) { remove(goodSeqFile.c_str()); return 0; }
+ if (m->control_pressed) { m->mothurRemove(goodSeqFile); return 0; }
}else{
processIDS.resize(0);
//append alignment and report files
for(int i=1;i<processors;i++){
m->appendFiles((goodSeqFile + toString(processIDS[i]) + ".temp"), goodSeqFile);
- remove((goodSeqFile + toString(processIDS[i]) + ".temp").c_str());
+ m->mothurRemove((goodSeqFile + toString(processIDS[i]) + ".temp"));
m->appendFiles((badAccnosFile + toString(processIDS[i]) + ".temp"), badAccnosFile);
- remove((badAccnosFile + toString(processIDS[i]) + ".temp").c_str());
+ m->mothurRemove((badAccnosFile + toString(processIDS[i]) + ".temp"));
}
- if (m->control_pressed) { remove(goodSeqFile.c_str()); return 0; }
+ if (m->control_pressed) { m->mothurRemove(goodSeqFile); return 0; }
//read badSeqs in because root process doesnt know what other "bad" seqs the children found
ifstream inBad;
#else
numFastaSeqs = driver(lines[0], goodSeqFile, badAccnosFile, fastafile, badSeqNames);
- if (m->control_pressed) { remove(goodSeqFile.c_str()); return 0; }
+ if (m->control_pressed) { m->mothurRemove(goodSeqFile); return 0; }
#endif
if(namefile != "" && groupfile != "") {
screenNameGroupFile(badSeqNames);
- if (m->control_pressed) { remove(goodSeqFile.c_str()); return 0; }
+ if (m->control_pressed) { m->mothurRemove(goodSeqFile); return 0; }
}else if(namefile != "") {
screenNameGroupFile(badSeqNames);
- if (m->control_pressed) { remove(goodSeqFile.c_str()); return 0; }
+ if (m->control_pressed) { m->mothurRemove(goodSeqFile); return 0; }
}else if(groupfile != "") { screenGroupFile(badSeqNames); } // this screens just the group
- if (m->control_pressed) { remove(goodSeqFile.c_str()); return 0; }
+ if (m->control_pressed) { m->mothurRemove(goodSeqFile); return 0; }
if(alignreport != "") { screenAlignReport(badSeqNames); }
if(qualfile != "") { screenQual(badSeqNames); }
+ if(taxonomy != "") { screenTaxonomy(badSeqNames); }
- if (m->control_pressed) { remove(goodSeqFile.c_str()); return 0; }
+ if (m->control_pressed) { m->mothurRemove(goodSeqFile); return 0; }
#ifdef USE_MPI
}
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setQualFile(current); }
}
+
+ itTypes = outputTypes.find("taxonomy");
+ if (itTypes != outputTypes.end()) {
+ if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setTaxonomyFile(current); }
+ }
m->mothurOut("It took " + toString(time(NULL) - start) + " secs to screen " + toString(numFastaSeqs) + " sequences.");
m->mothurOutEndLine();
ofstream goodNameOut; m->openOutputFile(goodNameFile, goodNameOut);
while(!inputNames.eof()){
- if (m->control_pressed) { goodNameOut.close(); inputNames.close(); remove(goodNameFile.c_str()); return 0; }
+ if (m->control_pressed) { goodNameOut.close(); inputNames.close(); m->mothurRemove(goodNameFile); return 0; }
inputNames >> seqName >> seqList;
it = badSeqNames.find(seqName);
ofstream goodGroupOut; m->openOutputFile(goodGroupFile, goodGroupOut);
while(!inputGroups.eof()){
- if (m->control_pressed) { goodGroupOut.close(); inputGroups.close(); remove(goodNameFile.c_str()); remove(goodGroupFile.c_str()); return 0; }
+ if (m->control_pressed) { goodGroupOut.close(); inputGroups.close(); m->mothurRemove(goodNameFile); m->mothurRemove(goodGroupFile); return 0; }
inputGroups >> seqName >> group;
}
}
//***************************************************************************************************************
-int ScreenSeqsCommand::getSummary(vector<unsigned long int>& positions){
+int ScreenSeqsCommand::getSummary(vector<unsigned long long>& positions){
try {
vector<int> startPosition;
vector<int> ambigBases;
vector<int> longHomoPolymer;
- vector<unsigned long int> positions = m->divideFile(fastafile, processors);
+ vector<unsigned long long> positions = m->divideFile(fastafile, processors);
for (int i = 0; i < (positions.size()-1); i++) {
lines.push_back(new linePair(positions[i], positions[(i+1)]));
}
+
+#ifdef USE_MPI
+ MPI_Comm_rank(MPI_COMM_WORLD, &pid);
+
+ if (pid == 0) { //only one process should fix files
+ driverCreateSummary(startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, fastafile, lines[0]);
+ }
+
+ MPI_Barrier(MPI_COMM_WORLD); //make everyone wait
+#else
int numSeqs = 0;
#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
if(processors == 1){
numSeqs = driverCreateSummary(startPosition, endPosition, seqLength, ambigBases, longHomoPolymer, fastafile, lines[0]);
if (m->control_pressed) { return 0; }
#endif
-
+#endif
sort(startPosition.begin(), startPosition.end());
sort(endPosition.begin(), endPosition.end());
sort(seqLength.begin(), seqLength.end());
}
#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
- unsigned long int pos = in.tellg();
+ unsigned long long pos = in.tellg();
if ((pos == -1) || (pos >= filePos->end)) { break; }
#else
if (in.eof()) { break; }
for (int k = 0; k < tempNum; k++) { in >> temp; longHomoPolymer.push_back(temp); } m->gobble(in);
in.close();
- remove(tempFilename.c_str());
+ m->mothurRemove(tempFilename);
}
return num;
ofstream goodGroupOut; m->openOutputFile(goodGroupFile, goodGroupOut);
while(!inputGroups.eof()){
- if (m->control_pressed) { goodGroupOut.close(); inputGroups.close(); remove(goodGroupFile.c_str()); return 0; }
+ if (m->control_pressed) { goodGroupOut.close(); inputGroups.close(); m->mothurRemove(goodGroupFile); return 0; }
inputGroups >> seqName >> group;
it = badSeqNames.find(seqName);
m->gobble(inputGroups);
}
- if (m->control_pressed) { goodGroupOut.close(); inputGroups.close(); remove(goodGroupFile.c_str()); return 0; }
+ if (m->control_pressed) { goodGroupOut.close(); inputGroups.close(); m->mothurRemove(goodGroupFile); return 0; }
//we were unable to remove some of the bad sequences
if (badSeqNames.size() != 0) {
inputGroups.close();
goodGroupOut.close();
- if (m->control_pressed) { remove(goodGroupFile.c_str()); }
+ if (m->control_pressed) { m->mothurRemove(goodGroupFile); }
return 0;
}
while(!inputAlignReport.eof()){
- if (m->control_pressed) { goodAlignReportOut.close(); inputAlignReport.close(); remove(goodAlignReportFile.c_str()); return 0; }
+ if (m->control_pressed) { goodAlignReportOut.close(); inputAlignReport.close(); m->mothurRemove(goodAlignReportFile); return 0; }
inputAlignReport >> seqName;
it = badSeqNames.find(seqName);
m->gobble(inputAlignReport);
}
- if (m->control_pressed) { goodAlignReportOut.close(); inputAlignReport.close(); remove(goodAlignReportFile.c_str()); return 0; }
+ if (m->control_pressed) { goodAlignReportOut.close(); inputAlignReport.close(); m->mothurRemove(goodAlignReportFile); return 0; }
//we were unable to remove some of the bad sequences
if (badSeqNames.size() != 0) {
inputAlignReport.close();
goodAlignReportOut.close();
- if (m->control_pressed) { remove(goodAlignReportFile.c_str()); return 0; }
+ if (m->control_pressed) { m->mothurRemove(goodAlignReportFile); return 0; }
return 0;
}
//***************************************************************************************************************
+int ScreenSeqsCommand::screenTaxonomy(set<string> badSeqNames){
+ try {
+ ifstream input;
+ m->openInputFile(taxonomy, input);
+ string seqName, tax;
+ set<string>::iterator it;
+
+ string goodTaxFile = outputDir + m->getRootName(m->getSimpleName(taxonomy)) + "good" + m->getExtension(taxonomy);
+ outputNames.push_back(goodTaxFile); outputTypes["taxonomy"].push_back(goodTaxFile);
+ ofstream goodTaxOut; m->openOutputFile(goodTaxFile, goodTaxOut);
+
+ while(!input.eof()){
+ if (m->control_pressed) { goodTaxOut.close(); input.close(); m->mothurRemove(goodTaxFile); return 0; }
+
+ input >> seqName >> tax;
+ it = badSeqNames.find(seqName);
+
+ if(it != badSeqNames.end()){ badSeqNames.erase(it); }
+ else{
+ goodTaxOut << seqName << '\t' << tax << endl;
+ }
+ m->gobble(input);
+ }
+
+ if (m->control_pressed) { goodTaxOut.close(); input.close(); m->mothurRemove(goodTaxFile); return 0; }
+
+ //we were unable to remove some of the bad sequences
+ if (badSeqNames.size() != 0) {
+ for (it = badSeqNames.begin(); it != badSeqNames.end(); it++) {
+ m->mothurOut("Your taxonomy file does not include the sequence " + *it + " please correct.");
+ m->mothurOutEndLine();
+ }
+ }
+
+ input.close();
+ goodTaxOut.close();
+
+ if (m->control_pressed) { m->mothurRemove(goodTaxFile); return 0; }
+
+ return 0;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ScreenSeqsCommand", "screenTaxonomy");
+ exit(1);
+ }
+
+}
+//***************************************************************************************************************
+
int ScreenSeqsCommand::screenQual(set<string> badSeqNames){
try {
ifstream in;
while(!in.eof()){
- if (m->control_pressed) { goodQual.close(); in.close(); remove(goodQualFile.c_str()); return 0; }
+ if (m->control_pressed) { goodQual.close(); in.close(); m->mothurRemove(goodQualFile); return 0; }
string saveName = "";
string name = "";
}
}
- if (m->control_pressed) { remove(goodQualFile.c_str()); return 0; }
+ if (m->control_pressed) { m->mothurRemove(goodQualFile); return 0; }
return 0;
}
#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux)
- unsigned long int pos = inFASTA.tellg();
+ unsigned long long pos = inFASTA.tellg();
if ((pos == -1) || (pos >= filePos->end)) { break; }
#else
if (inFASTA.eof()) { break; }
}
//**********************************************************************************************************************
#ifdef USE_MPI
-int ScreenSeqsCommand::driverMPI(int start, int num, MPI_File& inMPI, MPI_File& goodFile, MPI_File& badAccnosFile, vector<unsigned long int>& MPIPos, set<string>& badSeqNames){
+int ScreenSeqsCommand::driverMPI(int start, int num, MPI_File& inMPI, MPI_File& goodFile, MPI_File& badAccnosFile, vector<unsigned long long>& MPIPos, set<string>& badSeqNames){
try {
string outputString = "";
MPI_Status statusGood;
string tempFile = filename + toString(processIDS[i]) + ".num.temp";
m->openInputFile(tempFile, in);
if (!in.eof()) { int tempNum = 0; in >> tempNum; num += tempNum; }
- in.close(); remove(tempFile.c_str());
+ in.close(); m->mothurRemove(tempFile);
}
return num;
projects / mothur.git / blobdiff