#include "reversecommand.h"
#include "sequence.hpp"
+#include "qualityscores.h"
//**********************************************************************************************************************
vector<string> ReverseSeqsCommand::getValidParameters(){
try {
- string Array[] = {"fasta", "outputdir","inputdir"};
+ string Array[] = {"fasta", "qfile", "outputdir", "inputdir"};
vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
return myArray;
}
abort = true; calledHelp = true;
vector<string> tempOutNames;
outputTypes["fasta"] = tempOutNames;
+ outputTypes["qfile"] = tempOutNames;
}
catch(exception& e) {
m->errorOut(e, "ReverseSeqsCommand", "ReverseSeqsCommand");
}
}
//**********************************************************************************************************************
+
vector<string> ReverseSeqsCommand::getRequiredParameters(){
try {
- string Array[] = {"fasta"};
+ string Array[] = {"fasta", "qfile"};
vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
return myArray;
}
exit(1);
}
}
+
//**********************************************************************************************************************
+
vector<string> ReverseSeqsCommand::getRequiredFiles(){
try {
vector<string> myArray;
m->errorOut(e, "ReverseSeqsCommand", "getRequiredFiles");
exit(1);
}
-}
-//***************************************************************************************************************
+}//***************************************************************************************************************
ReverseSeqsCommand::ReverseSeqsCommand(string option) {
try {
else {
//valid paramters for this command
- string Array[] = {"fasta", "outputdir","inputdir"};
+ string Array[] = {"fasta", "qfile", "outputdir","inputdir"};
vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
OptionParser parser(option);
//initialize outputTypes
vector<string> tempOutNames;
outputTypes["fasta"] = tempOutNames;
-
+ outputTypes["qfile"] = tempOutNames;
+
//if the user changes the input directory command factory will send this info to us in the output parameter
string inputDir = validParameter.validFile(parameters, "inputdir", false);
if (inputDir == "not found"){ inputDir = ""; }
}
//check for required parameters
- fasta = validParameter.validFile(parameters, "fasta", true);
- if (fasta == "not open") { abort = true; }
- else if (fasta == "not found") { fasta = ""; m->mothurOut("fasta is a required parameter for the reverse.seqs command."); m->mothurOutEndLine(); abort = true; }
+ fastaFileName = validParameter.validFile(parameters, "fasta", true);
+ if (fastaFileName == "not open") { abort = true; }
+ else if (fastaFileName == "not found") { fastaFileName = "";}// m->mothurOut("fasta is a required parameter for the reverse.seqs command."); m->mothurOutEndLine(); abort = true; }
+
+ qualFileName = validParameter.validFile(parameters, "qfile", true);
+ if (qualFileName == "not open") { abort = true; }
+ else if (qualFileName == "not found") { qualFileName = ""; }//m->mothurOut("fasta is a required parameter for the reverse.seqs command."); m->mothurOutEndLine(); abort = true; }
+
+ if(fastaFileName == "not open" && qualFileName == "not open"){
+ m->mothurOut("fasta or qfile is a required parameter for the reverse.seqs command.");
+ m->mothurOutEndLine();
+ abort = true;
+ }
//if the user changes the output directory command factory will send this info to us in the output parameter
outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){
outputDir = "";
- outputDir += m->hasPath(fasta); //if user entered a file with a path then preserve it
+ outputDir += m->hasPath(fastaFileName); //if user entered a file with a path then preserve it
}
}
void ReverseSeqsCommand::help(){
try {
m->mothurOut("The reverse.seqs command reads a fastafile and outputs a fasta file containing the reverse compliment.\n");
- m->mothurOut("The reverse.seqs command parameter is fasta and it is required.\n");
+ m->mothurOut("The reverse.seqs command parameters fasta or qfile are required.\n");
m->mothurOut("The reverse.seqs command should be in the following format: \n");
m->mothurOut("reverse.seqs(fasta=yourFastaFile) \n");
}
if (abort == true) { if (calledHelp) { return 0; } return 2; }
- ifstream inFASTA;
- m->openInputFile(fasta, inFASTA);
-
- ofstream outFASTA;
- string reverseFile = outputDir + m->getRootName(m->getSimpleName(fasta)) + "rc" + m->getExtension(fasta);
- m->openOutputFile(reverseFile, outFASTA);
+ string fastaReverseFileName;
- while(!inFASTA.eof()){
- if (m->control_pressed) { inFASTA.close(); outFASTA.close(); remove(reverseFile.c_str()); return 0; }
-
- Sequence currSeq(inFASTA); m->gobble(inFASTA);
- if (currSeq.getName() != "") {
- currSeq.reverseComplement();
- currSeq.printSequence(outFASTA);
+ if(fastaFileName != ""){
+ ifstream inFASTA;
+ m->openInputFile(fastaFileName, inFASTA);
+
+ ofstream outFASTA;
+ fastaReverseFileName = outputDir + m->getRootName(m->getSimpleName(fastaFileName)) + "rc" + m->getExtension(fastaFileName);
+ m->openOutputFile(fastaReverseFileName, outFASTA);
+
+ while(!inFASTA.eof()){
+ if (m->control_pressed) { inFASTA.close(); outFASTA.close(); remove(fastaReverseFileName.c_str()); return 0; }
+
+ Sequence currSeq(inFASTA); m->gobble(inFASTA);
+ if (currSeq.getName() != "") {
+ currSeq.reverseComplement();
+ currSeq.printSequence(outFASTA);
+ }
}
+ inFASTA.close();
+ outFASTA.close();
+ outputNames.push_back(fastaReverseFileName);
}
- inFASTA.close();
- outFASTA.close();
- if (m->control_pressed) { remove(reverseFile.c_str()); return 0; }
+ string qualReverseFileName;
+
+ if(qualFileName != ""){
+ QualityScores currQual;
+
+ ifstream inQual;
+ m->openInputFile(qualFileName, inQual);
+
+ ofstream outQual;
+ string qualReverseFileName = outputDir + m->getRootName(m->getSimpleName(qualFileName)) + "rc" + m->getExtension(qualFileName);
+ m->openOutputFile(qualReverseFileName, outQual);
+
+ while(!inQual.eof()){
+ if (m->control_pressed) { inQual.close(); outQual.close(); remove(qualReverseFileName.c_str()); return 0; }
+ currQual = QualityScores(inQual); m->gobble(inQual);
+ currQual.flipQScores();
+ currQual.printQScores(outQual);
+ }
+ inQual.close();
+ outQual.close();
+ outputNames.push_back(qualReverseFileName);
+ }
m->mothurOutEndLine();
m->mothurOut("Output File Name: "); m->mothurOutEndLine();
- m->mothurOut(reverseFile); m->mothurOutEndLine(); outputNames.push_back(reverseFile); outputTypes["fasta"].push_back(reverseFile);
- m->mothurOutEndLine();
-
+ for(int i=0;i<outputNames.size();i++){
+ m->mothurOut(outputNames[i]);
+ m->mothurOutEndLine();
+ }
return 0;
-
}
catch(exception& e) {
m->errorOut(e, "ReverseSeqsCommand", "execute");
projects / mothur.git / blobdiff