//**********************************************************************************************************************
vector<string> RemoveSeqsCommand::setParameters(){
try {
+ CommandParameter pfastq("fastq", "InputTypes", "", "", "none", "FNGLT", "none","fastq",false,false,true); parameters.push_back(pfastq);
CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "FNGLT", "none","fasta",false,false,true); parameters.push_back(pfasta);
CommandParameter pname("name", "InputTypes", "", "", "NameCount", "FNGLT", "none","name",false,false,true); parameters.push_back(pname);
CommandParameter pcount("count", "InputTypes", "", "", "NameCount-CountGroup", "FNGLT", "none","count",false,false,true); parameters.push_back(pcount);
string RemoveSeqsCommand::getHelpString(){
try {
string helpString = "";
- helpString += "The remove.seqs command reads an .accnos file and at least one of the following file types: fasta, name, group, count, list, taxonomy, quality or alignreport file.\n";
+ helpString += "The remove.seqs command reads an .accnos file and at least one of the following file types: fasta, name, group, count, list, taxonomy, quality, fastq or alignreport file.\n";
helpString += "It outputs a file containing the sequences NOT in the .accnos file.\n";
- helpString += "The remove.seqs command parameters are accnos, fasta, name, group, count, list, taxonomy, qfile, alignreport and dups. You must provide accnos and at least one of the file parameters.\n";
+ helpString += "The remove.seqs command parameters are accnos, fasta, name, group, count, list, taxonomy, qfile, alignreport, fastq and dups. You must provide accnos and at least one of the file parameters.\n";
helpString += "The dups parameter allows you to remove the entire line from a name file if you remove any name from the line. default=true. \n";
helpString += "The remove.seqs command should be in the following format: remove.seqs(accnos=yourAccnos, fasta=yourFasta).\n";
helpString += "Example remove.seqs(accnos=amazon.accnos, fasta=amazon.fasta).\n";
string pattern = "";
if (type == "fasta") { pattern = "[filename],pick,[extension]"; }
+ else if (type == "fastq") { pattern = "[filename],pick,[extension]"; }
else if (type == "taxonomy") { pattern = "[filename],pick,[extension]"; }
else if (type == "name") { pattern = "[filename],pick,[extension]"; }
else if (type == "group") { pattern = "[filename],pick,[extension]"; }
setParameters();
vector<string> tempOutNames;
outputTypes["fasta"] = tempOutNames;
+ outputTypes["fastq"] = tempOutNames;
outputTypes["taxonomy"] = tempOutNames;
outputTypes["name"] = tempOutNames;
outputTypes["group"] = tempOutNames;
//initialize outputTypes
vector<string> tempOutNames;
outputTypes["fasta"] = tempOutNames;
+ outputTypes["fastq"] = tempOutNames;
outputTypes["taxonomy"] = tempOutNames;
outputTypes["name"] = tempOutNames;
outputTypes["group"] = tempOutNames;
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["count"] = inputDir + it->second; }
}
+
+ it = parameters.find("fastq");
+ //user has given a template file
+ if(it != parameters.end()){
+ path = m->hasPath(it->second);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { parameters["fastq"] = inputDir + it->second; }
+ }
}
if (qualfile == "not open") { abort = true; }
else if (qualfile == "not found") { qualfile = ""; }
else { m->setQualFile(qualfile); }
+
+ fastqfile = validParameter.validFile(parameters, "fastq", true);
+ if (fastqfile == "not open") { abort = true; }
+ else if (fastqfile == "not found") { fastqfile = ""; }
string usedDups = "true";
string temp = validParameter.validFile(parameters, "dups", false);
m->mothurOut("[ERROR]: you may only use one of the following: group or count."); m->mothurOutEndLine(); abort=true;
}
- if ((countfile == "") && (fastafile == "") && (namefile == "") && (groupfile == "") && (alignfile == "") && (listfile == "") && (taxfile == "") && (qualfile == "")) { m->mothurOut("You must provide at least one of the following: fasta, name, group, taxonomy, quality, alignreport or list."); m->mothurOutEndLine(); abort = true; }
+ if ((fastqfile == "") && (countfile == "") && (fastafile == "") && (namefile == "") && (groupfile == "") && (alignfile == "") && (listfile == "") && (taxfile == "") && (qualfile == "")) { m->mothurOut("You must provide at least one of the following: fasta, name, group, taxonomy, quality, alignreport, fastq or list."); m->mothurOutEndLine(); abort = true; }
if (countfile == "") {
if ((fastafile != "") && (namefile == "")) {
//read through the correct file and output lines you want to keep
if (namefile != "") { readName(); }
if (fastafile != "") { readFasta(); }
+ if (fastqfile != "") { readFastq(); }
if (groupfile != "") { readGroup(); }
if (alignfile != "") { readAlign(); }
if (listfile != "") { readList(); }
}
}
//**********************************************************************************************************************
+int RemoveSeqsCommand::readFastq(){
+ try {
+ bool wroteSomething = false;
+ int removedCount = 0;
+
+ ifstream in;
+ m->openInputFile(fastqfile, in);
+
+ string thisOutputDir = outputDir;
+ if (outputDir == "") { thisOutputDir += m->hasPath(fastqfile); }
+ map<string, string> variables;
+ variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(fastqfile));
+ variables["[extension]"] = m->getExtension(fastqfile);
+ string outputFileName = getOutputFileName("fastq", variables);
+ ofstream out;
+ m->openOutputFile(outputFileName, out);
+
+
+ while(!in.eof()){
+
+ if (m->control_pressed) { in.close(); out.close(); m->mothurRemove(outputFileName); return 0; }
+
+ //read sequence name
+ string input = m->getline(in); m->gobble(in);
+
+ string outputString = input + "\n";
+
+ if (input[0] == '@') {
+ //get rest of lines
+ outputString += m->getline(in) + "\n"; m->gobble(in);
+ outputString += m->getline(in) + "\n"; m->gobble(in);
+ outputString += m->getline(in) + "\n"; m->gobble(in);
+
+ vector<string> splits = m->splitWhiteSpace(input);
+ string name = splits[0];
+ name = name.substr(1);
+ m->checkName(name);
+
+ if (names.count(name) == 0) {
+ wroteSomething = true;
+ out << outputString;
+ }else { removedCount++; }
+ }
+
+ m->gobble(in);
+ }
+ in.close();
+ out.close();
+
+
+ if (wroteSomething == false) { m->mothurOut("Your file contains only sequences from the .accnos file."); m->mothurOutEndLine(); }
+ outputTypes["fasta"].push_back(outputFileName); outputNames.push_back(outputFileName);
+
+ m->mothurOut("Removed " + toString(removedCount) + " sequences from your fastq file."); m->mothurOutEndLine();
+
+
+ return 0;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "RemoveSeqsCommand", "readFastq");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
int RemoveSeqsCommand::readQual(){
try {
string thisOutputDir = outputDir;
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