]> git.donarmstrong.com Git - mothur.git/blobdiff - removegroupscommand.cpp
changes while testing
[mothur.git] / removegroupscommand.cpp
index 81722dd3e4047fc2505d64372a2ca539978c9445..64f9ab6822301310e975e6bbe58736e953eed4f2 100644 (file)
 //**********************************************************************************************************************
 vector<string> RemoveGroupsCommand::setParameters(){   
        try {
-               CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "FNGLT", "none",false,false); parameters.push_back(pfasta);
-               CommandParameter pshared("shared", "InputTypes", "", "", "none", "FNGLT-sharedGroup", "none",false,false); parameters.push_back(pshared);
-               CommandParameter pname("name", "InputTypes", "", "", "none", "FNGLT", "none",false,false); parameters.push_back(pname);
-               CommandParameter pgroup("group", "InputTypes", "", "", "none", "FNGLT-sharedGroup", "none",false,false); parameters.push_back(pgroup);
-               CommandParameter plist("list", "InputTypes", "", "", "none", "FNGLT", "none",false,false); parameters.push_back(plist);
-               CommandParameter ptaxonomy("taxonomy", "InputTypes", "", "", "none", "FNGLT", "none",false,false); parameters.push_back(ptaxonomy);
-               CommandParameter paccnos("accnos", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(paccnos);
-               CommandParameter pgroups("groups", "String", "", "", "", "", "",false,false); parameters.push_back(pgroups);
-               CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
-               CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir);
+               CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "none", "FNGLT","fasta",false,false,true); parameters.push_back(pfasta);
+               CommandParameter pshared("shared", "InputTypes", "", "", "none", "sharedGroup", "none","shared",false,false,true); parameters.push_back(pshared);
+        CommandParameter pname("name", "InputTypes", "", "", "NameCount", "none", "none","name",false,false,true); parameters.push_back(pname);
+        CommandParameter pcount("count", "InputTypes", "", "", "NameCount-CountGroup", "none", "none","count",false,false,true); parameters.push_back(pcount);
+               CommandParameter pgroup("group", "InputTypes", "", "", "CountGroup", "sharedGroup", "FNGLT","group",false,false,true); parameters.push_back(pgroup);            
+        CommandParameter pdesign("design", "InputTypes", "", "", "none", "sharedGroup", "FNGLT","design",false,false); parameters.push_back(pdesign);
+               CommandParameter plist("list", "InputTypes", "", "", "none", "none", "FNGLT","list",false,false,true); parameters.push_back(plist);
+               CommandParameter ptaxonomy("taxonomy", "InputTypes", "", "", "none", "none", "FNGLT","taxonomy",false,false,true); parameters.push_back(ptaxonomy);
+               CommandParameter paccnos("accnos", "InputTypes", "", "", "none", "none", "none","",false,false); parameters.push_back(paccnos);
+               CommandParameter pgroups("groups", "String", "", "", "", "", "","",false,false); parameters.push_back(pgroups);
+               CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
+               CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
                
                vector<string> myArray;
                for (int i = 0; i < parameters.size(); i++) {   myArray.push_back(parameters[i].name);          }
@@ -40,9 +42,9 @@ vector<string> RemoveGroupsCommand::setParameters(){
 string RemoveGroupsCommand::getHelpString(){   
        try {
                string helpString = "";
-               helpString += "The remove.groups command removes sequences from a specfic group or set of groups from the following file types: fasta, name, group, list, taxonomy or sharedfile.\n";
+               helpString += "The remove.groups command removes sequences from a specfic group or set of groups from the following file types: fasta, name, group, count, list, taxonomy, design or sharedfile.\n";
                helpString += "It outputs a file containing the sequences NOT in the those specified groups, or with a sharedfile eliminates the groups you selected.\n";
-               helpString += "The remove.groups command parameters are accnos, fasta, name, group, list, taxonomy, shared and groups. The group parameter is required, unless you have a current group file or are using a sharedfile.\n";
+               helpString += "The remove.groups command parameters are accnos, fasta, name, group, list, taxonomy, shared, design and groups. The group or count parameter is required, unless you have a current group or count file or are using a sharedfile.\n";
                helpString += "You must also provide an accnos containing the list of groups to remove or set the groups parameter to the groups you wish to remove.\n";
                helpString += "The groups parameter allows you to specify which of the groups in your groupfile you would like removed.  You can separate group names with dashes.\n";
                helpString += "The remove.groups command should be in the following format: remove.groups(accnos=yourAccnos, fasta=yourFasta, group=yourGroupFile).\n";
@@ -56,7 +58,28 @@ string RemoveGroupsCommand::getHelpString(){
                exit(1);
        }
 }
-
+//**********************************************************************************************************************
+string RemoveGroupsCommand::getOutputPattern(string type) {
+    try {
+        string pattern = "";
+        
+        if (type == "fasta")            {   pattern = "[filename],pick,[extension]";    }
+        else if (type == "taxonomy")    {   pattern = "[filename],pick,[extension]";    }
+        else if (type == "name")        {   pattern = "[filename],pick,[extension]";    }
+        else if (type == "group")       {   pattern = "[filename],pick,[extension]";    }
+        else if (type == "count")       {   pattern = "[filename],pick,[extension]";    }
+        else if (type == "list")        {   pattern = "[filename],pick,[extension]";    }
+        else if (type == "shared")      {   pattern = "[filename],[tag],pick,[extension]";    }
+        else if (type == "design")      {   pattern = "[filename],pick,[extension]";    }
+        else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->control_pressed = true;  }
+        
+        return pattern;
+    }
+    catch(exception& e) {
+        m->errorOut(e, "RemoveGroupsCommand", "getOutputPattern");
+        exit(1);
+    }
+}
 //**********************************************************************************************************************
 RemoveGroupsCommand::RemoveGroupsCommand(){    
        try {
@@ -69,6 +92,8 @@ RemoveGroupsCommand::RemoveGroupsCommand(){
                outputTypes["group"] = tempOutNames;
                outputTypes["list"] = tempOutNames;
                outputTypes["shared"] = tempOutNames;
+        outputTypes["design"] = tempOutNames;
+        outputTypes["count"] = tempOutNames;
        }
        catch(exception& e) {
                m->errorOut(e, "RemoveGroupsCommand", "RemoveGroupsCommand");
@@ -106,6 +131,8 @@ RemoveGroupsCommand::RemoveGroupsCommand(string option)  {
                        outputTypes["group"] = tempOutNames;
                        outputTypes["list"] = tempOutNames;
                        outputTypes["shared"] = tempOutNames;
+            outputTypes["design"] = tempOutNames;
+            outputTypes["count"] = tempOutNames;
                        
                        
                        //if the user changes the output directory command factory will send this info to us in the output parameter 
@@ -171,6 +198,22 @@ RemoveGroupsCommand::RemoveGroupsCommand(string option)  {
                                        //if the user has not given a path then, add inputdir. else leave path alone.
                                        if (path == "") {       parameters["shared"] = inputDir + it->second;           }
                                }
+                
+                it = parameters.find("design");
+                               //user has given a template file
+                               if(it != parameters.end()){ 
+                                       path = m->hasPath(it->second);
+                                       //if the user has not given a path then, add inputdir. else leave path alone.
+                                       if (path == "") {       parameters["design"] = inputDir + it->second;           }
+                               }
+                
+                it = parameters.find("count");
+                               //user has given a template file
+                               if(it != parameters.end()){ 
+                                       path = m->hasPath(it->second);
+                                       //if the user has not given a path then, add inputdir. else leave path alone.
+                                       if (path == "") {       parameters["count"] = inputDir + it->second;            }
+                               }
                        }
                        
                        
@@ -190,6 +233,11 @@ RemoveGroupsCommand::RemoveGroupsCommand(string option)  {
                        else if (namefile == "not found") {  namefile = "";  }  
                        else { m->setNameFile(namefile); }
                        
+                       groupfile = validParameter.validFile(parameters, "group", true);
+                       if (groupfile == "not open") { groupfile = "";  abort = true; }
+                       else if (groupfile == "not found") {    groupfile = "";         }
+                       else { m->setGroupFile(groupfile); }    
+                       
                        listfile = validParameter.validFile(parameters, "list", true);
                        if (listfile == "not open") { listfile = ""; abort = true; }
                        else if (listfile == "not found") {  listfile = "";  }
@@ -199,6 +247,11 @@ RemoveGroupsCommand::RemoveGroupsCommand(string option)  {
                        if (taxfile == "not open") { taxfile = ""; abort = true; }
                        else if (taxfile == "not found") {  taxfile = "";  }
                        else { m->setTaxonomyFile(taxfile); }
+            
+            designfile = validParameter.validFile(parameters, "design", true);
+                       if (designfile == "not open") { designfile = ""; abort = true; }
+                       else if (designfile == "not found") {  designfile = "";  }
+                       else { m->setDesignFile(designfile); }
                        
                        groups = validParameter.validFile(parameters, "groups", false);                 
                        if (groups == "not found") { groups = ""; }
@@ -212,12 +265,22 @@ RemoveGroupsCommand::RemoveGroupsCommand(string option)  {
                        else if (sharedfile == "not found") {  sharedfile = "";  }
                        else { m->setSharedFile(sharedfile); }
                        
-                       groupfile = validParameter.validFile(parameters, "group", true);
-                       if (groupfile == "not open") { groupfile = ""; abort = true; }
-                       else if (groupfile == "not found") {    groupfile = ""; }
-                       else { m->setGroupFile(groupfile); }    
                        
-                       if ((sharedfile == "") && (groupfile == "")) { 
+                       countfile = validParameter.validFile(parameters, "count", true);
+            if (countfile == "not open") { countfile = ""; abort = true; }
+            else if (countfile == "not found") { countfile = "";  }    
+            else { m->setCountTableFile(countfile); }
+            
+            if ((namefile != "") && (countfile != "")) {
+                m->mothurOut("[ERROR]: you may only use one of the following: name or count."); m->mothurOutEndLine(); abort = true;
+            }
+            
+            if ((groupfile != "") && (countfile != "")) {
+                m->mothurOut("[ERROR]: you may only use one of the following: group or count."); m->mothurOutEndLine(); abort=true;
+            }
+            
+                       
+                       if ((sharedfile == "") && (groupfile == "") && (designfile == "") && (countfile == "")) { 
                                //is there are current file available for any of these?
                                if ((namefile != "") || (fastafile != "") || (listfile != "") || (taxfile != "")) {
                                        //give priority to group, then shared
@@ -227,7 +290,11 @@ RemoveGroupsCommand::RemoveGroupsCommand(string option)  {
                                                sharedfile = m->getSharedFile(); 
                                                if (sharedfile != "") { m->mothurOut("Using " + sharedfile + " as input file for the shared parameter."); m->mothurOutEndLine(); }
                                                else { 
-                                                       m->mothurOut("You have no current groupfile or sharedfile and one is required."); m->mothurOutEndLine(); abort = true;
+                                                       countfile = m->getCountTableFile(); 
+                            if (countfile != "") { m->mothurOut("Using " + countfile + " as input file for the count parameter."); m->mothurOutEndLine(); }
+                            else { 
+                                m->mothurOut("You have no current groupfile, countfile or sharedfile and one is required."); m->mothurOutEndLine(); abort = true;
+                            }
                                                }
                                        }
                                }else {
@@ -238,7 +305,16 @@ RemoveGroupsCommand::RemoveGroupsCommand(string option)  {
                                                groupfile = m->getGroupFile(); 
                                                if (groupfile != "") { m->mothurOut("Using " + groupfile + " as input file for the group parameter."); m->mothurOutEndLine(); }
                                                else { 
-                                                       m->mothurOut("You have no current groupfile or sharedfile and one is required."); m->mothurOutEndLine(); abort = true;
+                                                       designfile = m->getDesignFile(); 
+                            if (designfile != "") { m->mothurOut("Using " + designfile + " as input file for the design parameter."); m->mothurOutEndLine(); }
+                            else { 
+                                countfile = m->getCountTableFile(); 
+                                if (countfile != "") { m->mothurOut("Using " + countfile + " as input file for the count parameter."); m->mothurOutEndLine(); }
+                                else { 
+                                    m->mothurOut("You have no current groupfile, designfile, countfile or sharedfile and one is required."); m->mothurOutEndLine(); abort = true;
+                                }
+                                
+                            }
                                                }
                                        }
                                }
@@ -246,8 +322,15 @@ RemoveGroupsCommand::RemoveGroupsCommand(string option)  {
                        
                        if ((accnosfile == "") && (Groups.size() == 0)) { m->mothurOut("You must provide an accnos file containing group names or specify groups using the groups parameter."); m->mothurOutEndLine(); abort = true; }
                        
-                       if ((fastafile == "") && (namefile == "") && (groupfile == "")  && (sharedfile == "") && (listfile == "") && (taxfile == ""))  { m->mothurOut("You must provide at least one of the following: fasta, name, taxonomy, group, shared or list."); m->mothurOutEndLine(); abort = true; }
-                       if ((groupfile == "") && ((namefile != "") || (fastafile != "") || (listfile != "") || (taxfile != "")))  { m->mothurOut("If using a fasta, name, taxonomy, group or list, then you must provide a group file."); m->mothurOutEndLine(); abort = true; }
+                       if ((fastafile == "") && (namefile == "") && (countfile == "") && (groupfile == "")  && (designfile == "") && (sharedfile == "") && (listfile == "") && (taxfile == ""))  { m->mothurOut("You must provide at least one of the following: fasta, name, taxonomy, group, shared, design, count or list."); m->mothurOutEndLine(); abort = true; }
+                       if (((groupfile == "") && (countfile == "")) && ((namefile != "") || (fastafile != "") || (listfile != "") || (taxfile != "")))  { m->mothurOut("If using a fasta, name, taxonomy, group or list, then you must provide a group or count file."); m->mothurOutEndLine(); abort = true; }
+            
+            if (countfile == "") {
+                if ((namefile == "") && ((fastafile != "") || (taxfile != ""))){
+                    vector<string> files; files.push_back(fastafile); files.push_back(taxfile);
+                    parser.getNameFile(files);
+                }
+            }
                }
                
        }
@@ -264,7 +347,7 @@ int RemoveGroupsCommand::execute(){
                if (abort == true) { if (calledHelp) { return 0; }  return 2;   }
                
                //get groups you want to remove
-               if (accnosfile != "") { readAccnos(); }
+               if (accnosfile != "") { m->readAccnos(accnosfile, Groups); m->setGroups(Groups);  }
                
                if (groupfile != "") {
                        groupMap = new GroupMap(groupfile);
@@ -272,16 +355,45 @@ int RemoveGroupsCommand::execute(){
                        
                        //make sure groups are valid
                        //takes care of user setting groupNames that are invalid or setting groups=all
-                       SharedUtil* util = new SharedUtil();
                        vector<string> namesGroups = groupMap->getNamesOfGroups();
-                       util->setGroups(Groups, namesGroups);
-                       delete util;
-                       
+                       vector<string> checkedGroups;
+            for (int i = 0; i < Groups.size(); i++) {
+                if (m->inUsersGroups(Groups[i], namesGroups)) { checkedGroups.push_back(Groups[i]); }
+                else {  m->mothurOut("[WARNING]: " + Groups[i] + " is not a valid group in your groupfile, ignoring.\n"); }
+            }
+            
+            if (checkedGroups.size() == 0) { m->mothurOut("[ERROR]: no valid groups, aborting.\n"); delete groupMap; return 0; }
+                       else {
+                Groups = checkedGroups;
+                m->setGroups(Groups);
+            }
+            
                        //fill names with names of sequences that are from the groups we want to remove 
                        fillNames();
                        
                        delete groupMap;
-               }
+               }else if (countfile != ""){
+            if ((fastafile != "") || (listfile != "") || (taxfile != "")) { 
+                m->mothurOut("\n[NOTE]: The count file should contain only unique names, so mothur assumes your fasta, list and taxonomy files also contain only uniques.\n\n");
+            }
+            CountTable ct;
+            ct.readTable(countfile, true);
+            if (!ct.hasGroupInfo()) { m->mothurOut("[ERROR]: your count file does not contain group info, aborting.\n"); return 0; }
+            
+            vector<string> gNamesOfGroups = ct.getNamesOfGroups();
+            SharedUtil util;
+            util.setGroups(Groups, gNamesOfGroups);
+            vector<string> namesOfSeqs = ct.getNamesOfSeqs();
+            sort(Groups.begin(), Groups.end());
+            
+            for (int i = 0; i < namesOfSeqs.size(); i++) {
+                vector<string> thisSeqsGroups = ct.getGroups(namesOfSeqs[i]);
+                if (m->isSubset(Groups, thisSeqsGroups)) { //you only have seqs from these groups so remove you
+                    names.insert(namesOfSeqs[i]);
+                }
+            }
+        }
+
                                
                if (m->control_pressed) { return 0; }
                
@@ -289,9 +401,11 @@ int RemoveGroupsCommand::execute(){
                if (namefile != "")                     {               readName();             }
                if (fastafile != "")            {               readFasta();    }
                if (groupfile != "")            {               readGroup();    }
+        if (countfile != "")           {               readCount();    }
                if (listfile != "")                     {               readList();             }
                if (taxfile != "")                      {               readTax();              }
                if (sharedfile != "")           {               readShared();   }
+        if (designfile != "")          {               readDesign();   }
                
                if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) {        m->mothurRemove(outputNames[i]); } return 0; }
                                
@@ -332,6 +446,16 @@ int RemoveGroupsCommand::execute(){
                        if (itTypes != outputTypes.end()) {
                                if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setSharedFile(current); }
                        }
+            
+            itTypes = outputTypes.find("design");
+                       if (itTypes != outputTypes.end()) {
+                               if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setDesignFile(current); }
+                       }
+            
+            itTypes = outputTypes.find("count");
+                       if (itTypes != outputTypes.end()) {
+                               if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setCountTableFile(current); }
+                       }
                }
                
                return 0;               
@@ -348,7 +472,10 @@ int RemoveGroupsCommand::readFasta(){
        try {
                string thisOutputDir = outputDir;
                if (outputDir == "") {  thisOutputDir += m->hasPath(fastafile);  }
-               string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(fastafile)) + "pick" + m->getExtension(fastafile);
+        map<string, string> variables; 
+        variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(fastafile));
+        variables["[extension]"] = m->getExtension(fastafile);
+               string outputFileName = getOutputFileName("fasta", variables);
                
                ofstream out;
                m->openOutputFile(outputFileName, out);
@@ -370,9 +497,16 @@ int RemoveGroupsCommand::readFasta(){
                                //if this name is in the accnos file
                                if (names.count(name) == 0) {
                                        wroteSomething = true;
-                                       
-                                       currSeq.printSequence(out);
-                               }else { removedCount++; }
+                                       currSeq.printSequence(out); 
+                               }else { 
+                                       //if you are not in the accnos file check if you are a name that needs to be changed
+                                       map<string, string>::iterator it = uniqueToRedundant.find(name);
+                                       if (it != uniqueToRedundant.end()) {
+                                               wroteSomething = true;
+                                               currSeq.setName(it->second);
+                                               currSeq.printSequence(out);
+                                       }else { removedCount++; }
+                               }
                        }
                        m->gobble(in);
                }
@@ -402,6 +536,10 @@ int RemoveGroupsCommand::readShared(){
                //that way we can take advantage of the reads in inputdata and sharedRabundVector
                InputData* tempInput = new InputData(sharedfile, "sharedfile");
                vector<SharedRAbundVector*> lookup = tempInput->getSharedRAbundVectors();
+        
+        map<string, string> variables; 
+        variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(sharedfile));
+        variables["[extension]"] = m->getExtension(sharedfile);
        
                //save m->Groups
                vector<string> allGroupsNames = m->getAllGroups();
@@ -421,7 +559,6 @@ int RemoveGroupsCommand::readShared(){
                delete tempInput;
                m->setGroups(groupsToKeep);
                m->clearAllGroups();
-               m->names.clear();
                m->saveNextLabel = "";
                m->printedHeaders = false;
                m->currentBinLabels.clear();
@@ -434,7 +571,8 @@ int RemoveGroupsCommand::readShared(){
                
                while(lookup[0] != NULL) {
                        
-                       string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(sharedfile)) + lookup[0]->getLabel() + ".pick" + m->getExtension(sharedfile);
+                       variables["[tag]"] = lookup[0]->getLabel();
+            string outputFileName = getOutputFileName("shared", variables);
                        ofstream out;
                        m->openOutputFile(outputFileName, out);
                        outputTypes["shared"].push_back(outputFileName);  outputNames.push_back(outputFileName);
@@ -482,7 +620,11 @@ int RemoveGroupsCommand::readList(){
        try {
                string thisOutputDir = outputDir;
                if (outputDir == "") {  thisOutputDir += m->hasPath(listfile);  }
-               string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(listfile)) + "pick" +  m->getExtension(listfile);
+               map<string, string> variables; 
+        variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(listfile));
+        variables["[extension]"] = m->getExtension(listfile);
+               string outputFileName = getOutputFileName("list", variables);
+
                
                ofstream out;
                m->openOutputFile(outputFileName, out);
@@ -518,12 +660,23 @@ int RemoveGroupsCommand::readList(){
                                        
                                        //if that name is in the .accnos file, add it
                                        if (names.count(name) == 0) {  newNames += name + ",";  }
-                                       else { removedCount++; }
+                                       else {
+                                               //if you are not in the accnos file check if you are a name that needs to be changed
+                                               map<string, string>::iterator it = uniqueToRedundant.find(name);
+                                               if (it != uniqueToRedundant.end()) {
+                                                       newNames += it->second + ",";
+                                               }else { removedCount++; }
+                                       }
                                }
                                
                                //get last name
                                if (names.count(binnames) == 0) {  newNames += binnames + ",";  }
-                               else { removedCount++; }
+                               else { //if you are not in the accnos file check if you are a name that needs to be changed
+                                       map<string, string>::iterator it = uniqueToRedundant.find(binnames);
+                                       if (it != uniqueToRedundant.end()) {
+                                               newNames += it->second + ",";
+                                       }else { removedCount++; }
+                               }
                                
                                //if there are names in this bin add to new list
                                if (newNames != "") {  
@@ -561,8 +714,10 @@ int RemoveGroupsCommand::readName(){
        try {
                string thisOutputDir = outputDir;
                if (outputDir == "") {  thisOutputDir += m->hasPath(namefile);  }
-               string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(namefile)) + "pick" + m->getExtension(namefile);
-               
+               map<string, string> variables; 
+               variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(namefile));
+        variables["[extension]"] = m->getExtension(namefile);
+               string outputFileName = getOutputFileName("name", variables);   
                ofstream out;
                m->openOutputFile(outputFileName, out);
                
@@ -615,6 +770,7 @@ int RemoveGroupsCommand::readName(){
                                        //you know you have at least one valid second since first column is valid
                                        for (int i = 0; i < validSecond.size()-1; i++) {  out << validSecond[i] << ',';  }
                                        out << validSecond[validSecond.size()-1] << endl;
+                                       uniqueToRedundant[firstCol] = validSecond[0];
                                }
                        }
                        
@@ -641,8 +797,10 @@ int RemoveGroupsCommand::readGroup(){
        try {
                string thisOutputDir = outputDir;
                if (outputDir == "") {  thisOutputDir += m->hasPath(groupfile);  }
-               string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(groupfile)) + "pick" + m->getExtension(groupfile);
-               
+        map<string, string> variables; 
+               variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(groupfile));
+        variables["[extension]"] = m->getExtension(groupfile);
+               string outputFileName = getOutputFileName("group", variables);  
                ofstream out;
                m->openOutputFile(outputFileName, out);
                
@@ -684,17 +842,105 @@ int RemoveGroupsCommand::readGroup(){
        }
 }
 //**********************************************************************************************************************
-int RemoveGroupsCommand::readTax(){
+int RemoveGroupsCommand::readCount(){
        try {
                string thisOutputDir = outputDir;
-               if (outputDir == "") {  thisOutputDir += m->hasPath(taxfile);  }
-               string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(taxfile)) + "pick" + m->getExtension(taxfile);
+               if (outputDir == "") {  thisOutputDir += m->hasPath(countfile);  }
+               map<string, string> variables; 
+               variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(countfile));
+        variables["[extension]"] = m->getExtension(countfile);
+               string outputFileName = getOutputFileName("count", variables);
+               
                ofstream out;
                m->openOutputFile(outputFileName, out);
                
                ifstream in;
-               m->openInputFile(taxfile, in);
-               string name, tax;
+               m->openInputFile(countfile, in);
+               
+               bool wroteSomething = false;
+               int removedCount = 0;
+               
+        string headers = m->getline(in); m->gobble(in);
+        vector<string> columnHeaders = m->splitWhiteSpace(headers);
+        
+        vector<string> groups;
+        map<int, string> originalGroupIndexes;
+        map<string, int> GroupIndexes;
+        set<int> indexOfGroupsChosen;
+        for (int i = 2; i < columnHeaders.size(); i++) {  groups.push_back(columnHeaders[i]);  originalGroupIndexes[i-2] = columnHeaders[i]; }
+        //sort groups to keep consistent with how we store the groups in groupmap
+        sort(groups.begin(), groups.end());
+        for (int i = 0; i < groups.size(); i++) {  GroupIndexes[groups[i]] = i; }
+
+               vector<string> groupsToKeep;
+               for (int i = 0; i < groups.size(); i++) {
+                       if (!m->inUsersGroups(groups[i], Groups)) { groupsToKeep.push_back(groups[i]); }
+               }
+        sort(groupsToKeep.begin(), groupsToKeep.end());
+        out << "Representative_Sequence\ttotal\t";
+        for (int i = 0; i < groupsToKeep.size(); i++) { out << groupsToKeep[i] << '\t'; indexOfGroupsChosen.insert(GroupIndexes[groupsToKeep[i]]); }
+        out << endl;
+        
+        string name; int oldTotal;
+        while (!in.eof()) {
+            
+            if (m->control_pressed) { in.close();  out.close();  m->mothurRemove(outputFileName);  return 0; }
+            
+            in >> name; m->gobble(in); in >> oldTotal; m->gobble(in);
+            if (m->debug) { m->mothurOut("[DEBUG]: " + name + '\t' + toString(oldTotal) + "\n"); }
+            
+            if (names.count(name) == 0) {
+                //if group info, then read it
+                vector<int> selectedCounts; int thisTotal = 0; int temp;
+                for (int i = 0; i < groups.size(); i++) {  
+                    int thisIndex = GroupIndexes[originalGroupIndexes[i]]; 
+                    in >> temp;  m->gobble(in);
+                    if (indexOfGroupsChosen.count(thisIndex) != 0) { //we want this group
+                        selectedCounts.push_back(temp); thisTotal += temp;
+                    }
+                }
+                
+                out << name << '\t' << thisTotal << '\t';
+                for (int i = 0; i < selectedCounts.size(); i++) {  out << selectedCounts[i] << '\t'; }
+                out << endl;
+                
+                wroteSomething = true;
+                removedCount+= (oldTotal - thisTotal);
+            }else {  m->getline(in); removedCount += oldTotal; }
+            
+            m->gobble(in);
+        }
+        in.close();
+               out.close();
+               
+               if (wroteSomething == false) {  m->mothurOut("Your file does NOT contain sequences from the groups you wish to get."); m->mothurOutEndLine();  }
+               outputTypes["count"].push_back(outputFileName); outputNames.push_back(outputFileName);
+               
+               m->mothurOut("Removed " + toString(removedCount) + " sequences from your count file."); m->mothurOutEndLine();
+        
+               return 0;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "RemoveGroupsCommand", "readCount");
+               exit(1);
+       }
+}
+//**********************************************************************************************************************
+int RemoveGroupsCommand::readDesign(){
+       try {
+               string thisOutputDir = outputDir;
+               if (outputDir == "") {  thisOutputDir += m->hasPath(designfile);  }
+        map<string, string> variables; 
+               variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(designfile));
+        variables["[extension]"] = m->getExtension(designfile);
+               string outputFileName = getOutputFileName("design", variables);
+               
+               ofstream out;
+               m->openOutputFile(outputFileName, out);
+               
+               ifstream in;
+               m->openInputFile(designfile, in);
+               string name, group;
                
                bool wroteSomething = false;
                int removedCount = 0;
@@ -703,12 +949,12 @@ int RemoveGroupsCommand::readTax(){
                        if (m->control_pressed) { in.close();  out.close();  m->mothurRemove(outputFileName);  return 0; }
                        
                        in >> name;                             //read from first column
-                       in >> tax;                      //read from second column
+                       in >> group;                    //read from second column
                        
                        //if this name is in the accnos file
-                       if (names.count(name) == 0) {
+                       if (!(m->inUsersGroups(name, Groups))) {
                                wroteSomething = true;
-                               out << name << '\t' << tax << endl;
+                               out << name << '\t' << group << endl;
                        }else {  removedCount++;  }
                        
                        m->gobble(in);
@@ -716,41 +962,70 @@ int RemoveGroupsCommand::readTax(){
                in.close();
                out.close();
                
-               if (wroteSomething == false) {  m->mothurOut("Your file contains only sequences from the groups you wish to remove."); m->mothurOutEndLine();  }
-               outputTypes["taxonomy"].push_back(outputFileName); outputNames.push_back(outputFileName);
+               if (wroteSomething == false) {  m->mothurOut("Your file contains only groups from the groups you wish to remove."); m->mothurOutEndLine();  }
+               outputTypes["design"].push_back(outputFileName); outputNames.push_back(outputFileName);
                
-               m->mothurOut("Removed " + toString(removedCount) + " sequences from your taxonomy file."); m->mothurOutEndLine();
+               m->mothurOut("Removed " + toString(removedCount) + " groups from your design file."); m->mothurOutEndLine();
+        
                
                return 0;
        }
        catch(exception& e) {
-               m->errorOut(e, "RemoveGroupsCommand", "readTax");
+               m->errorOut(e, "RemoveGroupsCommand", "readDesign");
                exit(1);
        }
 }
+
 //**********************************************************************************************************************
-void RemoveGroupsCommand::readAccnos(){
+int RemoveGroupsCommand::readTax(){
        try {
-               Groups.clear();
+               string thisOutputDir = outputDir;
+               if (outputDir == "") {  thisOutputDir += m->hasPath(taxfile);  }
+               map<string, string> variables; 
+               variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(taxfile));
+        variables["[extension]"] = m->getExtension(taxfile);
+               string outputFileName = getOutputFileName("taxonomy", variables);
+               ofstream out;
+               m->openOutputFile(outputFileName, out);
                
                ifstream in;
-               m->openInputFile(accnosfile, in);
-               string name;
+               m->openInputFile(taxfile, in);
+               string name, tax;
+               
+               bool wroteSomething = false;
+               int removedCount = 0;
                
                while(!in.eof()){
-                       in >> name;
+                       if (m->control_pressed) { in.close();  out.close();  m->mothurRemove(outputFileName);  return 0; }
                        
-                       Groups.push_back(name);
+                       in >> name;                             //read from first column
+                       in >> tax;                      //read from second column
+                       
+                       //if this name is in the accnos file
+                       if (names.count(name) == 0) {
+                               wroteSomething = true;
+                               out << name << '\t' << tax << endl;
+                       }else {  //if you are not in the accnos file check if you are a name that needs to be changed
+                               map<string, string>::iterator it = uniqueToRedundant.find(name);
+                               if (it != uniqueToRedundant.end()) {
+                                       wroteSomething = true;
+                                       out << it->second << '\t' << tax << endl;
+                               }else { removedCount++; }  }
                        
                        m->gobble(in);
                }
-               in.close();     
+               in.close();
+               out.close();
                
-               m->setGroups(Groups);
+               if (wroteSomething == false) {  m->mothurOut("Your file contains only sequences from the groups you wish to remove."); m->mothurOutEndLine();  }
+               outputTypes["taxonomy"].push_back(outputFileName); outputNames.push_back(outputFileName);
+               
+               m->mothurOut("Removed " + toString(removedCount) + " sequences from your taxonomy file."); m->mothurOutEndLine();
                
+               return 0;
        }
        catch(exception& e) {
-               m->errorOut(e, "RemoveGroupsCommand", "readAccnos");
+               m->errorOut(e, "RemoveGroupsCommand", "readTax");
                exit(1);
        }
 }