#include "rarefactsharedcommand.h"
#include "sharedsobs.h"
#include "sharednseqs.h"
+#include "sharedutilities.h"
+#include "subsample.h"
+//**********************************************************************************************************************
+vector<string> RareFactSharedCommand::setParameters(){
+ try {
+ CommandParameter pshared("shared", "InputTypes", "", "", "none", "none", "none","",false,true,true); parameters.push_back(pshared);
+ CommandParameter pdesign("design", "InputTypes", "", "", "none", "none", "none","",false,false); parameters.push_back(pdesign);
+ CommandParameter plabel("label", "String", "", "", "", "", "","",false,false); parameters.push_back(plabel);
+ CommandParameter pfreq("freq", "Number", "", "100", "", "", "","",false,false); parameters.push_back(pfreq);
+ CommandParameter piters("iters", "Number", "", "1000", "", "", "","",false,false); parameters.push_back(piters);
+ CommandParameter pcalc("calc", "Multiple", "sharednseqs-sharedobserved", "sharedobserved", "", "", "","",true,false,true); parameters.push_back(pcalc);
+ CommandParameter psubsampleiters("subsampleiters", "Number", "", "1000", "", "", "","",false,false); parameters.push_back(psubsampleiters);
+ CommandParameter psubsample("subsample", "String", "", "", "", "", "","",false,false); parameters.push_back(psubsample);
+ CommandParameter pjumble("jumble", "Boolean", "", "T", "", "", "","",false,false); parameters.push_back(pjumble);
+ CommandParameter pgroups("groups", "String", "", "", "", "", "","",false,false); parameters.push_back(pgroups);
+ CommandParameter psets("sets", "String", "", "", "", "", "","",false,false); parameters.push_back(psets);
+ CommandParameter pgroupmode("groupmode", "Boolean", "", "T", "", "", "","",false,false); parameters.push_back(pgroupmode);
+ CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
+ CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
+
+ vector<string> myArray;
+ for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); }
+ return myArray;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "RareFactSharedCommand", "setParameters");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+string RareFactSharedCommand::getHelpString(){
+ try {
+ string helpString = "";
+ ValidCalculators validCalculator;
+ helpString += "The rarefaction.shared command parameters are shared, design, label, iters, groups, sets, jumble, groupmode and calc. shared is required if there is no current sharedfile. \n";
+ helpString += "The design parameter allows you to assign your groups to sets. If provided mothur will run rarefaction.shared on a per set basis. \n";
+ helpString += "The sets parameter allows you to specify which of the sets in your designfile you would like to analyze. The set names are separated by dashes. THe default is all sets in the designfile.\n";
+ helpString += "The rarefaction command should be in the following format: \n";
+ helpString += "rarefaction.shared(label=yourLabel, iters=yourIters, calc=yourEstimators, jumble=yourJumble, groups=yourGroups).\n";
+ helpString += "The freq parameter is used indicate when to output your data, by default it is set to 100. But you can set it to a percentage of the number of sequence. For example freq=0.10, means 10%. \n";
+ helpString += "Example rarefaction.shared(label=unique-0.01-0.03, iters=10000, groups=B-C, jumble=T, calc=sharedobserved).\n";
+ helpString += "The default values for iters is 1000, freq is 100, and calc is sharedobserved which calculates the shared rarefaction curve for the observed richness.\n";
+ helpString += "The subsampleiters parameter allows you to choose the number of times you would like to run the subsample.\n";
+ helpString += "The subsample parameter allows you to enter the size pergroup of the sample or you can set subsample=T and mothur will use the size of your smallest group.\n";
+ helpString += "The default value for groups is all the groups in your groupfile, and jumble is true.\n";
+ helpString += validCalculator.printCalc("sharedrarefaction");
+ helpString += "The label parameter is used to analyze specific labels in your input.\n";
+ helpString += "The groups parameter allows you to specify which of the groups in your groupfile you would like analyzed. You must enter at least 2 valid groups.\n";
+ helpString += "Note: No spaces between parameter labels (i.e. freq), '=' and parameters (i.e.yourFreq).\n";
+ return helpString;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "RareFactSharedCommand", "getHelpString");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+string RareFactSharedCommand::getOutputPattern(string type) {
+ try {
+ string pattern = "";
+
+ if (type == "sharedrarefaction") { pattern = "[filename],shared.rarefaction"; }
+ else if (type == "sharedr_nseqs") { pattern = "[filename],shared.r_nseqs"; }
+ else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->control_pressed = true; }
+
+ return pattern;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "RareFactSharedCommand", "getOutputPattern");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+RareFactSharedCommand::RareFactSharedCommand(){
+ try {
+ abort = true; calledHelp = true;
+ setParameters();
+ vector<string> tempOutNames;
+ outputTypes["sharedrarefaction"] = tempOutNames;
+ outputTypes["sharedr_nseqs"] = tempOutNames;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "RareFactSharedCommand", "RareFactSharedCommand");
+ exit(1);
+ }
+}
//**********************************************************************************************************************
-RareFactSharedCommand::RareFactSharedCommand(){
+RareFactSharedCommand::RareFactSharedCommand(string option) {
try {
- globaldata = GlobalData::getInstance();
- string fileNameRoot;
- fileNameRoot = getRootName(globaldata->inputFileName);
- format = globaldata->getFormat();
- validCalculator = new ValidCalculators();
- util = new SharedUtil();
+ abort = false; calledHelp = false;
+ allLines = 1;
- int i;
- for (i=0; i<globaldata->Estimators.size(); i++) {
- if (validCalculator->isValidCalculator("sharedrarefaction", globaldata->Estimators[i]) == true) {
- if (globaldata->Estimators[i] == "sharedobserved") {
- rDisplays.push_back(new RareDisplay(new SharedSobs(), new SharedThreeColumnFile(fileNameRoot+"shared.rarefaction", "")));
- }else if (globaldata->Estimators[i] == "sharednseqs") {
- rDisplays.push_back(new RareDisplay(new SharedNSeqs(), new SharedThreeColumnFile(fileNameRoot+"shared.r_nseqs", "")));
+ //allow user to run help
+ if(option == "help") { help(); abort = true; calledHelp = true; }
+ else if(option == "citation") { citation(); abort = true; calledHelp = true;}
+
+ else {
+ vector<string> myArray = setParameters();
+
+ OptionParser parser(option);
+ map<string,string> parameters = parser.getParameters();
+ map<string,string>::iterator it;
+
+ ValidParameters validParameter;
+
+ //check to make sure all parameters are valid for command
+ for (it = parameters.begin(); it != parameters.end(); it++) {
+ if (validParameter.isValidParameter(it->first, myArray, it->second) != true) { abort = true; }
+ }
+
+ //initialize outputTypes
+ vector<string> tempOutNames;
+ outputTypes["sharedrarefaction"] = tempOutNames;
+ outputTypes["sharedr_nseqs"] = tempOutNames;
+
+ //if the user changes the input directory command factory will send this info to us in the output parameter
+ string inputDir = validParameter.validFile(parameters, "inputdir", false);
+ if (inputDir == "not found"){ inputDir = ""; }
+ else {
+ string path;
+ it = parameters.find("shared");
+ //user has given a template file
+ if(it != parameters.end()){
+ path = m->hasPath(it->second);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { parameters["shared"] = inputDir + it->second; }
+ }
+
+ it = parameters.find("design");
+ //user has given a template file
+ if(it != parameters.end()){
+ path = m->hasPath(it->second);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { parameters["design"] = inputDir + it->second; }
}
}
+
+ //get shared file
+ sharedfile = validParameter.validFile(parameters, "shared", true);
+ if (sharedfile == "not open") { sharedfile = ""; abort = true; }
+ else if (sharedfile == "not found") {
+ //if there is a current shared file, use it
+ sharedfile = m->getSharedFile();
+ if (sharedfile != "") { m->mothurOut("Using " + sharedfile + " as input file for the shared parameter."); m->mothurOutEndLine(); }
+ else { m->mothurOut("You have no current sharedfile and the shared parameter is required."); m->mothurOutEndLine(); abort = true; }
+ }else { m->setSharedFile(sharedfile); }
+
+ designfile = validParameter.validFile(parameters, "design", true);
+ if (designfile == "not open") { abort = true; designfile = ""; }
+ else if (designfile == "not found") { designfile = ""; }
+ else { m->setDesignFile(designfile); }
+
+
+ //if the user changes the output directory command factory will send this info to us in the output parameter
+ outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){ outputDir = m->hasPath(sharedfile); }
+
+
+ //check for optional parameter and set defaults
+ // ...at some point should added some additional type checking...
+ label = validParameter.validFile(parameters, "label", false);
+ if (label == "not found") { label = ""; }
+ else {
+ if(label != "all") { m->splitAtDash(label, labels); allLines = 0; }
+ else { allLines = 1; }
+ }
+
+
+ calc = validParameter.validFile(parameters, "calc", false);
+ if (calc == "not found") { calc = "sharedobserved"; }
+ else {
+ if (calc == "default") { calc = "sharedobserved"; }
+ }
+ m->splitAtDash(calc, Estimators);
+ if (m->inUsersGroups("citation", Estimators)) {
+ ValidCalculators validCalc; validCalc.printCitations(Estimators);
+ //remove citation from list of calcs
+ for (int i = 0; i < Estimators.size(); i++) { if (Estimators[i] == "citation") { Estimators.erase(Estimators.begin()+i); break; } }
+ }
+
+ groups = validParameter.validFile(parameters, "groups", false);
+ if (groups == "not found") { groups = ""; }
+ else {
+ m->splitAtDash(groups, Groups);
+ }
+ m->setGroups(Groups);
+
+ string sets = validParameter.validFile(parameters, "sets", false);
+ if (sets == "not found") { sets = ""; }
+ else {
+ m->splitAtDash(sets, Sets);
+ }
+
+ string temp;
+ temp = validParameter.validFile(parameters, "freq", false); if (temp == "not found") { temp = "100"; }
+ m->mothurConvert(temp, freq);
+
+ temp = validParameter.validFile(parameters, "iters", false); if (temp == "not found") { temp = "1000"; }
+ m->mothurConvert(temp, nIters);
+
+ temp = validParameter.validFile(parameters, "jumble", false); if (temp == "not found") { temp = "T"; }
+ if (m->isTrue(temp)) { jumble = true; }
+ else { jumble = false; }
+ m->jumble = jumble;
+
+ temp = validParameter.validFile(parameters, "groupmode", false); if (temp == "not found") { temp = "T"; }
+ groupMode = m->isTrue(temp);
+
+ temp = validParameter.validFile(parameters, "subsampleiters", false); if (temp == "not found") { temp = "1000"; }
+ m->mothurConvert(temp, iters);
+
+ temp = validParameter.validFile(parameters, "subsample", false); if (temp == "not found") { temp = "F"; }
+ if (m->isNumeric1(temp)) { m->mothurConvert(temp, subsampleSize); subsample = true; }
+ else {
+ if (m->isTrue(temp)) { subsample = true; subsampleSize = -1; } //we will set it to smallest group later
+ else { subsample = false; }
+ }
+
+ if (subsample == false) { iters = 1; }
+
}
-
- //reset calc for next command
- globaldata->setCalc("");
}
catch(exception& e) {
- cout << "Standard Error: " << e.what() << " has occurred in the RareFactSharedCommand class Function RareFactSharedCommand. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ m->errorOut(e, "RareFactSharedCommand", "RareFactSharedCommand");
exit(1);
}
- catch(...) {
- cout << "An unknown error has occurred in the RareFactSharedCommand class function RareFactSharedCommand. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
- exit(1);
- }
-
}
-
//**********************************************************************************************************************
-RareFactSharedCommand::~RareFactSharedCommand(){
- delete order;
- delete input;
- delete rCurve;
- delete read;
- delete util;
-}
+int RareFactSharedCommand::execute(){
+ try {
+
+ if (abort == true) { if (calledHelp) { return 0; } return 2; }
+
+ GroupMap designMap;
+ if (designfile == "") { //fake out designMap to run with process
+ process(designMap, "");
+ }else {
+ designMap.readDesignMap(designfile);
+
+ //fill Sets - checks for "all" and for any typo groups
+ SharedUtil util;
+ vector<string> nameSets = designMap.getNamesOfGroups();
+ util.setGroups(Sets, nameSets);
+
+ for (int i = 0; i < Sets.size(); i++) {
+ process(designMap, Sets[i]);
+ }
+
+ if (groupMode) { outputNames = createGroupFile(outputNames); }
+ }
+
+ if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; }
+
+ m->mothurOutEndLine();
+ m->mothurOut("Output File Names: "); m->mothurOutEndLine();
+ for (int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i]); m->mothurOutEndLine(); }
+ m->mothurOutEndLine();
+ return 0;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "RareFactSharedCommand", "execute");
+ exit(1);
+ }
+}
//**********************************************************************************************************************
-int RareFactSharedCommand::execute(){
+int RareFactSharedCommand::process(GroupMap& designMap, string thisSet){
try {
- int count = 1;
-
- //if the users entered no valid calculators don't execute command
- if (rDisplays.size() == 0) { return 0; }
+ Rarefact* rCurve;
+ vector<Display*> rDisplays;
+
+ InputData input(sharedfile, "sharedfile");
+ lookup = input.getSharedRAbundVectors();
+ if (lookup.size() < 2) {
+ m->mothurOut("I cannot run the command without at least 2 valid groups.");
+ for (int i = 0; i < lookup.size(); i++) { delete lookup[i]; }
+ return 0;
+ }
+
+ string fileNameRoot = outputDir + m->getRootName(m->getSimpleName(sharedfile));
+
+ vector<string> newGroups = m->getGroups();
+ if (thisSet != "") { //make groups only filled with groups from this set so that's all inputdata will read
+ vector<string> thisSets; thisSets.push_back(thisSet);
+ newGroups = designMap.getNamesSeqs(thisSets);
+ fileNameRoot += thisSet + ".";
+ }
+
+ vector<SharedRAbundVector*> subset;
+ if (thisSet == "") { subset.clear(); subset = lookup; }
+ else {//fill subset with this sets groups
+ subset.clear();
+ for (int i = 0; i < lookup.size(); i++) {
+ if (m->inUsersGroups(lookup[i]->getGroup(), newGroups)) {
+ subset.push_back(lookup[i]);
+ }
+ }
+ }
- if (format == "sharedfile") {
- read = new ReadPhilFile(globaldata->inputFileName);
- read->read(&*globaldata);
-
- input = globaldata->ginput;
- order = input->getSharedOrderVector();
- }else {
- //you are using a list and a groupfile
- read = new ReadPhilFile(globaldata->inputFileName);
- read->read(&*globaldata);
-
- input = globaldata->ginput;
- SharedList = globaldata->gSharedList;
- order = SharedList->getSharedOrderVector();
+ /******************************************************/
+ if (subsample) {
+ if (subsampleSize == -1) { //user has not set size, set size = smallest samples size
+ subsampleSize = subset[0]->getNumSeqs();
+ for (int i = 1; i < subset.size(); i++) {
+ int thisSize = subset[i]->getNumSeqs();
+
+ if (thisSize < subsampleSize) { subsampleSize = thisSize; }
+ }
+ }else {
+ newGroups.clear();
+ vector<SharedRAbundVector*> temp;
+ for (int i = 0; i < subset.size(); i++) {
+ if (subset[i]->getNumSeqs() < subsampleSize) {
+ m->mothurOut(subset[i]->getGroup() + " contains " + toString(subset[i]->getNumSeqs()) + ". Eliminating."); m->mothurOutEndLine();
+ delete subset[i];
+ }else {
+ newGroups.push_back(subset[i]->getGroup());
+ temp.push_back(subset[i]);
+ }
+ }
+ subset = temp;
+ }
+
+ if (subset.size() < 2) { m->mothurOut("You have not provided enough valid groups. I cannot run the command."); m->mothurOutEndLine(); m->control_pressed = true; return 0; }
+ }
+ /******************************************************/
+
+ map<string, string> variables;
+ variables["[filename]"] = fileNameRoot;
+ ValidCalculators validCalculator;
+ for (int i=0; i<Estimators.size(); i++) {
+ if (validCalculator.isValidCalculator("sharedrarefaction", Estimators[i]) == true) {
+ if (Estimators[i] == "sharedobserved") {
+ rDisplays.push_back(new RareDisplay(new SharedSobs(), new SharedThreeColumnFile(getOutputFileName("sharedrarefaction",variables), "")));
+ outputNames.push_back(getOutputFileName("sharedrarefaction",variables)); outputTypes["sharedrarefaction"].push_back(getOutputFileName("sharedrarefaction",variables));
+ }else if (Estimators[i] == "sharednseqs") {
+ rDisplays.push_back(new RareDisplay(new SharedNSeqs(), new SharedThreeColumnFile(getOutputFileName("sharedr_nseqs",variables), "")));
+ outputNames.push_back(getOutputFileName("sharedr_nseqs",variables)); outputTypes["sharedr_nseqs"].push_back(getOutputFileName("sharedr_nseqs",variables));
+ }
+ }
+ file2Group[outputNames.size()-1] = thisSet;
}
- //set users groups
- util->setGroups(globaldata->Groups, globaldata->gGroupmap->namesOfGroups, "rarefact");
-
- while(order != NULL){
+ //if the users entered no valid calculators don't execute command
+ if (rDisplays.size() == 0) { for (int i = 0; i < lookup.size(); i++) { delete lookup[i]; } return 0; }
- if(globaldata->allLines == 1 || globaldata->lines.count(count) == 1 || globaldata->labels.count(order->getLabel()) == 1){
- //create collectors curve
- rCurve = new Rarefact(order, rDisplays);
- convert(globaldata->getFreq(), freq);
- convert(globaldata->getIters(), nIters);
- rCurve->getSharedCurve(freq, nIters);
+ if (m->control_pressed) {
+ for(int i=0;i<rDisplays.size();i++){ delete rDisplays[i]; }
+ for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); }
+ for (int i = 0; i < lookup.size(); i++) { delete lookup[i]; }
+ return 0;
+ }
+
+
+ //if the users enters label "0.06" and there is no "0.06" in their file use the next lowest label.
+ string lastLabel = subset[0]->getLabel();
+ set<string> processedLabels;
+ set<string> userLabels = labels;
+
+ //as long as you are not at the end of the file or done wih the lines you want
+ while((subset[0] != NULL) && ((allLines == 1) || (userLabels.size() != 0))) {
+ if (m->control_pressed) {
+ for(int i=0;i<rDisplays.size();i++){ delete rDisplays[i]; }
+ for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); }
+ for (int i = 0; i < lookup.size(); i++) { delete lookup[i]; }
+ return 0;
+ }
+ if(allLines == 1 || labels.count(subset[0]->getLabel()) == 1){
+ m->mothurOut(subset[0]->getLabel() + '\t' + thisSet); m->mothurOutEndLine();
+ rCurve = new Rarefact(subset, rDisplays);
+ rCurve->getSharedCurve(freq, nIters);
delete rCurve;
+
+ if (subsample) { subsampleLookup(subset, fileNameRoot); }
+
+ processedLabels.insert(subset[0]->getLabel());
+ userLabels.erase(subset[0]->getLabel());
+ }
- cout << order->getLabel() << '\t' << count << endl;
+ if ((m->anyLabelsToProcess(subset[0]->getLabel(), userLabels, "") == true) && (processedLabels.count(lastLabel) != 1)) {
+ string saveLabel = subset[0]->getLabel();
+
+ for (int i = 0; i < lookup.size(); i++) { delete lookup[i]; }
+ lookup = input.getSharedRAbundVectors(lastLabel);
+
+ if (thisSet == "") { subset.clear(); subset = lookup; }
+ else {//fill subset with this sets groups
+ subset.clear();
+ for (int i = 0; i < lookup.size(); i++) {
+ if (m->inUsersGroups(lookup[i]->getGroup(), newGroups)) {
+ subset.push_back(lookup[i]);
+ }
+ }
+ }
+
+ m->mothurOut(subset[0]->getLabel() + '\t' + thisSet); m->mothurOutEndLine();
+ rCurve = new Rarefact(subset, rDisplays);
+ rCurve->getSharedCurve(freq, nIters);
+ delete rCurve;
+
+ if (subsample) { subsampleLookup(subset, fileNameRoot); }
+
+ processedLabels.insert(subset[0]->getLabel());
+ userLabels.erase(subset[0]->getLabel());
+
+ //restore real lastlabel to save below
+ subset[0]->setLabel(saveLabel);
}
+
+
+ lastLabel = subset[0]->getLabel();
//get next line to process
- if (format == "sharedfile") {
- order = input->getSharedOrderVector();
+ for (int i = 0; i < lookup.size(); i++) { delete lookup[i]; }
+ lookup = input.getSharedRAbundVectors();
+
+ if (lookup[0] != NULL) {
+ if (thisSet == "") { subset.clear(); subset = lookup; }
+ else {//fill subset with this sets groups
+ subset.clear();
+ for (int i = 0; i < lookup.size(); i++) {
+ if (m->inUsersGroups(lookup[i]->getGroup(), newGroups)) {
+ subset.push_back(lookup[i]);
+ }
+ }
+ }
+ }else { subset.clear(); subset.push_back(NULL); }
+
+ }
+
+ if (m->control_pressed) {
+ for(int i=0;i<rDisplays.size();i++){ delete rDisplays[i]; }
+ for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); }
+ return 0;
+ }
+
+ //output error messages about any remaining user labels
+ set<string>::iterator it;
+ bool needToRun = false;
+ for (it = userLabels.begin(); it != userLabels.end(); it++) {
+ m->mothurOut("Your file does not include the label " + *it);
+ if (processedLabels.count(lastLabel) != 1) {
+ m->mothurOut(". I will use " + lastLabel + "."); m->mothurOutEndLine();
+ needToRun = true;
}else {
- //you are using a list and a groupfile
- SharedList = input->getSharedListVector(); //get new list vector to process
- if (SharedList != NULL) {
- order = SharedList->getSharedOrderVector(); //gets new order vector with group info.
- }else {
- break;
- }
+ m->mothurOut(". Please refer to " + lastLabel + "."); m->mothurOutEndLine();
}
-
- count++;
}
-
- for(int i=0;i<rDisplays.size();i++){ delete rDisplays[i]; }
- //reset groups parameter
- globaldata->Groups.clear(); globaldata->setGroups("");
+ if (m->control_pressed) {
+ for(int i=0;i<rDisplays.size();i++){ delete rDisplays[i]; }
+ for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); }
+ return 0;
+ }
+
+ //run last label if you need to
+ if (needToRun == true) {
+ for (int i = 0; i < lookup.size(); i++) { if (lookup[i] != NULL) { delete lookup[i]; } }
+ lookup = input.getSharedRAbundVectors(lastLabel);
+
+ if (thisSet == "") { subset.clear(); subset = lookup; }
+ else {//fill subset with this sets groups
+ subset.clear();
+ for (int i = 0; i < lookup.size(); i++) {
+ if (m->inUsersGroups(lookup[i]->getGroup(), newGroups)) {
+ subset.push_back(lookup[i]);
+ }
+ }
+ }
+
+ m->mothurOut(subset[0]->getLabel() + '\t' + thisSet); m->mothurOutEndLine();
+ rCurve = new Rarefact(subset, rDisplays);
+ rCurve->getSharedCurve(freq, nIters);
+ delete rCurve;
+
+ if (subsample) { subsampleLookup(subset, fileNameRoot); }
+
+ for (int i = 0; i < lookup.size(); i++) { delete lookup[i]; }
+ }
+
+ for(int i=0;i<rDisplays.size();i++){ delete rDisplays[i]; }
- return 0;
+
+ return 0;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "RareFactSharedCommand", "process");
+ exit(1);
}
+}
+//**********************************************************************************************************************
+int RareFactSharedCommand::subsampleLookup(vector<SharedRAbundVector*>& thisLookup, string fileNameRoot) {
+ try {
+
+ map<string, vector<string> > filenames;
+ for (int thisIter = 0; thisIter < iters; thisIter++) {
+
+ vector<SharedRAbundVector*> thisItersLookup = thisLookup;
+
+ //we want the summary results for the whole dataset, then the subsampling
+ SubSample sample;
+ vector<string> tempLabels; //dont need since we arent printing the sampled sharedRabunds
+
+ //make copy of lookup so we don't get access violations
+ vector<SharedRAbundVector*> newLookup;
+ for (int k = 0; k < thisItersLookup.size(); k++) {
+ SharedRAbundVector* temp = new SharedRAbundVector();
+ temp->setLabel(thisItersLookup[k]->getLabel());
+ temp->setGroup(thisItersLookup[k]->getGroup());
+ newLookup.push_back(temp);
+ }
+
+ //for each bin
+ for (int k = 0; k < thisItersLookup[0]->getNumBins(); k++) {
+ if (m->control_pressed) { for (int j = 0; j < newLookup.size(); j++) { delete newLookup[j]; } return 0; }
+ for (int j = 0; j < thisItersLookup.size(); j++) { newLookup[j]->push_back(thisItersLookup[j]->getAbundance(k), thisItersLookup[j]->getGroup()); }
+ }
+
+ tempLabels = sample.getSample(newLookup, subsampleSize);
+ thisItersLookup = newLookup;
+
+
+ Rarefact* rCurve;
+ vector<Display*> rDisplays;
+
+ string thisfileNameRoot = fileNameRoot + toString(thisIter);
+
+ map<string, string> variables;
+ variables["[filename]"] = thisfileNameRoot;
+
+ ValidCalculators validCalculator;
+ for (int i=0; i<Estimators.size(); i++) {
+ if (validCalculator.isValidCalculator("sharedrarefaction", Estimators[i]) == true) {
+ if (Estimators[i] == "sharedobserved") {
+ rDisplays.push_back(new RareDisplay(new SharedSobs(), new SharedThreeColumnFile(getOutputFileName("sharedrarefaction",variables), "")));
+ filenames["sharedrarefaction"].push_back(getOutputFileName("sharedrarefaction",variables));
+ }else if (Estimators[i] == "sharednseqs") {
+ rDisplays.push_back(new RareDisplay(new SharedNSeqs(), new SharedThreeColumnFile(getOutputFileName("sharedr_nseqs",variables), "")));
+ filenames["sharedr_nseqs"].push_back(getOutputFileName("sharedr_nseqs",variables));
+ }
+ }
+ }
+
+ rCurve = new Rarefact(thisItersLookup, rDisplays);
+ rCurve->getSharedCurve(freq, nIters);
+ delete rCurve;
+
+ //clean up memory
+ for (int i = 0; i < thisItersLookup.size(); i++) { delete thisItersLookup[i]; }
+ thisItersLookup.clear();
+ for(int i=0;i<rDisplays.size();i++){ delete rDisplays[i]; }
+ }
+
+ //create std and ave outputs
+ vector< vector< vector< double > > > results; //iter -> numSampled -> data
+ for (map<string, vector<string> >::iterator it = filenames.begin(); it != filenames.end(); it++) {
+ vector<string> thisTypesFiles = it->second;
+ vector<string> columnHeaders;
+ for (int i = 0; i < thisTypesFiles.size(); i++) {
+ ifstream in;
+ m->openInputFile(thisTypesFiles[i], in);
+
+ string headers = m->getline(in); m->gobble(in);
+ columnHeaders = m->splitWhiteSpace(headers);
+ int numCols = columnHeaders.size();
+
+ vector<vector<double> > thisFilesLines;
+ while (!in.eof()) {
+ if (m->control_pressed) { break; }
+ vector<double> data; data.resize(numCols, 0);
+ //read numSampled line
+ for (int j = 0; j < numCols; j++) { in >> data[j]; m->gobble(in); }
+ thisFilesLines.push_back(data);
+ }
+ in.close();
+ results.push_back(thisFilesLines);
+ m->mothurRemove(thisTypesFiles[i]);
+ }
+
+ if (!m->control_pressed) {
+ //process results
+ map<string, string> variables; variables["[filename]"] = fileNameRoot + "ave-std." + thisLookup[0]->getLabel() + ".";
+
+ string outputFile = getOutputFileName(it->first,variables);
+ ofstream out;
+ m->openOutputFile(outputFile, out);
+ outputNames.push_back(outputFile); outputTypes[it->first].push_back(outputFile);
+
+ out << columnHeaders[0] << '\t' << "method\t";
+ for (int i = 1; i < columnHeaders.size(); i++) { out << columnHeaders[i] << '\t'; }
+ out << endl;
+
+ vector< vector<double> > aveResults; aveResults.resize(results[0].size());
+ for (int i = 0; i < aveResults.size(); i++) { aveResults[i].resize(results[0][i].size(), 0.0); }
+
+ for (int thisIter = 0; thisIter < iters; thisIter++) { //sum all groups dists for each calculator
+ for (int i = 0; i < aveResults.size(); i++) { //initialize sums to zero.
+ aveResults[i][0] = results[thisIter][i][0];
+ for (int j = 1; j < aveResults[i].size(); j++) {
+ aveResults[i][j] += results[thisIter][i][j];
+ }
+ }
+ }
+
+ for (int i = 0; i < aveResults.size(); i++) { //finds average.
+ for (int j = 1; j < aveResults[i].size(); j++) {
+ aveResults[i][j] /= (float) iters;
+ }
+ }
+
+ //standard deviation
+ vector< vector<double> > stdResults; stdResults.resize(results[0].size());
+ for (int i = 0; i < stdResults.size(); i++) { stdResults[i].resize(results[0][i].size(), 0.0); }
+
+ for (int thisIter = 0; thisIter < iters; thisIter++) { //compute the difference of each dist from the mean, and square the result of each
+ for (int i = 0; i < stdResults.size(); i++) {
+ stdResults[i][0] = aveResults[i][0];
+ for (int j = 1; j < stdResults[i].size(); j++) {
+ stdResults[i][j] += ((results[thisIter][i][j] - aveResults[i][j]) * (results[thisIter][i][j] - aveResults[i][j]));
+ }
+ }
+ }
+
+ for (int i = 0; i < stdResults.size(); i++) { //finds average.
+ out << aveResults[i][0] << '\t' << "ave\t";
+ for (int j = 1; j < aveResults[i].size(); j++) { out << aveResults[i][j] << '\t'; }
+ out << endl;
+ out << stdResults[i][0] << '\t' << "std\t";
+ for (int j = 1; j < stdResults[i].size(); j++) {
+ stdResults[i][j] /= (float) iters;
+ stdResults[i][j] = sqrt(stdResults[i][j]);
+ out << stdResults[i][j] << '\t';
+ }
+ out << endl;
+ }
+ out.close();
+ }
+ }
+
+
+ return 0;
+ }
catch(exception& e) {
- cout << "Standard Error: " << e.what() << " has occurred in the RareFactSharedCommand class Function execute. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ m->errorOut(e, "RareFactSharedCommand", "subsample");
exit(1);
}
- catch(...) {
- cout << "An unknown error has occurred in the RareFactSharedCommand class function execute. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+}
+//**********************************************************************************************************************
+vector<string> RareFactSharedCommand::createGroupFile(vector<string>& outputNames) {
+ try {
+
+ vector<string> newFileNames;
+
+ //find different types of files
+ map<string, map<string, string> > typesFiles;
+ map<string, vector< vector<string> > > fileLabels; //combofile name to labels. each label is a vector because it may be unique lci hci.
+ vector<string> groupNames;
+ for (int i = 0; i < outputNames.size(); i++) {
+
+ string extension = m->getExtension(outputNames[i]);
+ string combineFileName = outputDir + m->getRootName(m->getSimpleName(sharedfile)) + "groups" + extension;
+ m->mothurRemove(combineFileName); //remove old file
+
+ ifstream in;
+ m->openInputFile(outputNames[i], in);
+
+ string labels = m->getline(in);
+
+ istringstream iss (labels,istringstream::in);
+ string newLabel = ""; vector<string> theseLabels;
+ while(!iss.eof()) { iss >> newLabel; m->gobble(iss); theseLabels.push_back(newLabel); }
+ vector< vector<string> > allLabels;
+ vector<string> thisSet; thisSet.push_back(theseLabels[0]); allLabels.push_back(thisSet); thisSet.clear(); //makes "numSampled" its own grouping
+ for (int j = 1; j < theseLabels.size()-1; j++) {
+ if (theseLabels[j+1] == "lci") {
+ thisSet.push_back(theseLabels[j]);
+ thisSet.push_back(theseLabels[j+1]);
+ thisSet.push_back(theseLabels[j+2]);
+ j++; j++;
+ }else{ //no lci or hci for this calc.
+ thisSet.push_back(theseLabels[j]);
+ }
+ allLabels.push_back(thisSet);
+ thisSet.clear();
+ }
+ fileLabels[combineFileName] = allLabels;
+
+ map<string, map<string, string> >::iterator itfind = typesFiles.find(extension);
+ if (itfind != typesFiles.end()) {
+ (itfind->second)[outputNames[i]] = file2Group[i];
+ }else {
+ map<string, string> temp;
+ temp[outputNames[i]] = file2Group[i];
+ typesFiles[extension] = temp;
+ }
+ if (!(m->inUsersGroups(file2Group[i], groupNames))) { groupNames.push_back(file2Group[i]); }
+ }
+
+ //for each type create a combo file
+
+ for (map<string, map<string, string> >::iterator it = typesFiles.begin(); it != typesFiles.end(); it++) {
+
+ ofstream out;
+ string combineFileName = outputDir + m->getRootName(m->getSimpleName(sharedfile)) + "groups" + it->first;
+ m->openOutputFileAppend(combineFileName, out);
+ newFileNames.push_back(combineFileName);
+ map<string, string> thisTypesFiles = it->second; //it->second maps filename to group
+ set<int> numSampledSet;
+
+ //open each type summary file
+ map<string, map<int, vector< vector<string> > > > files; //maps file name to lines in file
+ int maxLines = 0;
+ for (map<string, string>::iterator itFileNameGroup = thisTypesFiles.begin(); itFileNameGroup != thisTypesFiles.end(); itFileNameGroup++) {
+
+ string thisfilename = itFileNameGroup->first;
+ string group = itFileNameGroup->second;
+
+ ifstream temp;
+ m->openInputFile(thisfilename, temp);
+
+ //read through first line - labels
+ m->getline(temp); m->gobble(temp);
+
+ map<int, vector< vector<string> > > thisFilesLines;
+ while (!temp.eof()){
+ int numSampled = 0;
+ temp >> numSampled; m->gobble(temp);
+
+ vector< vector<string> > theseReads;
+ vector<string> thisSet; thisSet.push_back(toString(numSampled)); theseReads.push_back(thisSet); thisSet.clear();
+ for (int k = 1; k < fileLabels[combineFileName].size(); k++) { //output thing like 0.03-A lci-A hci-A
+ vector<string> reads;
+ string next = "";
+ for (int l = 0; l < fileLabels[combineFileName][k].size(); l++) { //output modified labels
+ temp >> next; m->gobble(temp);
+ reads.push_back(next);
+ }
+ theseReads.push_back(reads);
+ }
+ thisFilesLines[numSampled] = theseReads;
+ m->gobble(temp);
+
+ numSampledSet.insert(numSampled);
+ }
+
+ files[group] = thisFilesLines;
+
+ //save longest file for below
+ if (maxLines < thisFilesLines.size()) { maxLines = thisFilesLines.size(); }
+
+ temp.close();
+ m->mothurRemove(thisfilename);
+ }
+
+ //output new labels line
+ out << fileLabels[combineFileName][0][0] << '\t';
+ for (int k = 1; k < fileLabels[combineFileName].size(); k++) { //output thing like 0.03-A lci-A hci-A
+ for (int n = 0; n < groupNames.size(); n++) { // for each group
+ for (int l = 0; l < fileLabels[combineFileName][k].size(); l++) { //output modified labels
+ out << fileLabels[combineFileName][k][l] << '-' << groupNames[n] << '\t';
+ }
+ }
+ }
+ out << endl;
+
+ //for each label
+ for (set<int>::iterator itNumSampled = numSampledSet.begin(); itNumSampled != numSampledSet.end(); itNumSampled++) {
+
+ out << (*itNumSampled) << '\t';
+
+ if (m->control_pressed) { break; }
+
+ for (int k = 1; k < fileLabels[combineFileName].size(); k++) { //each chunk
+ //grab data for each group
+ for (map<string, map<int, vector< vector<string> > > >::iterator itFileNameGroup = files.begin(); itFileNameGroup != files.end(); itFileNameGroup++) {
+
+ string group = itFileNameGroup->first;
+
+ map<int, vector< vector<string> > >::iterator itLine = files[group].find(*itNumSampled);
+ if (itLine != files[group].end()) {
+ for (int l = 0; l < (itLine->second)[k].size(); l++) {
+ out << (itLine->second)[k][l] << '\t';
+
+ }
+ }else {
+ for (int l = 0; l < fileLabels[combineFileName][k].size(); l++) {
+ out << "NA" << '\t';
+ }
+ }
+ }
+ }
+ out << endl;
+ }
+ out.close();
+ }
+
+ //return combine file name
+ return newFileNames;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "RareFactSharedCommand", "createGroupFile");
exit(1);
- }
+ }
}
-
-
//**********************************************************************************************************************
projects / mothur.git / blobdiff