]> git.donarmstrong.com Git - mothur.git/blobdiff - rarefactcommand.cpp
changes while testing
[mothur.git] / rarefactcommand.cpp
index 82251c4a8fad67ea36fea2d7232b2f9171d5e3f5..75a87efa3a2f95259488b0abf15887fb39ee1955 100644 (file)
 //**********************************************************************************************************************
 vector<string> RareFactCommand::setParameters(){       
        try {
-               CommandParameter plist("list", "InputTypes", "", "", "LRSS", "LRSS", "none",false,false); parameters.push_back(plist);
-               CommandParameter prabund("rabund", "InputTypes", "", "", "LRSS", "LRSS", "none",false,false); parameters.push_back(prabund);
-               CommandParameter psabund("sabund", "InputTypes", "", "", "LRSS", "LRSS", "none",false,false); parameters.push_back(psabund);
-               CommandParameter pshared("shared", "InputTypes", "", "", "LRSS", "LRSS", "none",false,false); parameters.push_back(pshared);
-               CommandParameter plabel("label", "String", "", "", "", "", "",false,false); parameters.push_back(plabel);
-               CommandParameter pfreq("freq", "Number", "", "100", "", "", "",false,false); parameters.push_back(pfreq);
-               CommandParameter piters("iters", "Number", "", "1000", "", "", "",false,false); parameters.push_back(piters);
-               CommandParameter pcalc("calc", "Multiple", "sobs-chao-nseqs-coverage-ace-jack-shannon-shannoneven-npshannon-heip-smithwilson-simpson-simpsoneven-invsimpson-bootstrap", "sobs", "", "", "",true,false); parameters.push_back(pcalc);
-               CommandParameter pabund("abund", "Number", "", "10", "", "", "",false,false); parameters.push_back(pabund);
-               CommandParameter pprocessors("processors", "Number", "", "1", "", "", "",false,false); parameters.push_back(pprocessors);
-               CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
-               CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir);
+               CommandParameter plist("list", "InputTypes", "", "", "LRSS", "LRSS", "none","",false,false,true); parameters.push_back(plist);
+               CommandParameter prabund("rabund", "InputTypes", "", "", "LRSS", "LRSS", "none","",false,false); parameters.push_back(prabund);
+               CommandParameter psabund("sabund", "InputTypes", "", "", "LRSS", "LRSS", "none","",false,false); parameters.push_back(psabund);
+               CommandParameter pshared("shared", "InputTypes", "", "", "LRSS", "LRSS", "none","",false,false,true); parameters.push_back(pshared);
+               CommandParameter plabel("label", "String", "", "", "", "", "","",false,false); parameters.push_back(plabel);
+               CommandParameter pfreq("freq", "Number", "", "100", "", "", "","",false,false); parameters.push_back(pfreq);
+               CommandParameter piters("iters", "Number", "", "1000", "", "", "","",false,false); parameters.push_back(piters);
+               CommandParameter pcalc("calc", "Multiple", "sobs-chao-nseqs-coverage-ace-jack-shannon-shannoneven-npshannon-heip-smithwilson-simpson-simpsoneven-invsimpson-bootstrap", "sobs", "", "", "","",true,false,true); parameters.push_back(pcalc);
+               CommandParameter pabund("abund", "Number", "", "10", "", "", "","",false,false); parameters.push_back(pabund);
+               CommandParameter pprocessors("processors", "Number", "", "1", "", "", "","",false,false,true); parameters.push_back(pprocessors);
+               CommandParameter pgroupmode("groupmode", "Boolean", "", "T", "", "", "","",false,false); parameters.push_back(pgroupmode);
+               CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
+               CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
                
                vector<string> myArray;
                for (int i = 0; i < parameters.size(); i++) {   myArray.push_back(parameters[i].name);          }
@@ -55,7 +56,7 @@ string RareFactCommand::getHelpString(){
        try {
                ValidCalculators validCalculator;
                string helpString = "";
-               helpString += "The rarefaction.single command parameters are list, sabund, rabund, shared, label, iters, freq, calc, processors and abund.  list, sabund, rabund or shared is required unless you have a valid current file. \n";
+               helpString += "The rarefaction.single command parameters are list, sabund, rabund, shared, label, iters, freq, calc, processors, groupmode and abund.  list, sabund, rabund or shared is required unless you have a valid current file. \n";
                helpString += "The freq parameter is used indicate when to output your data, by default it is set to 100. But you can set it to a percentage of the number of sequence. For example freq=0.10, means 10%. \n";
                helpString += "The processors parameter allows you to specify the number of processors to use. The default is 1.\n";
                helpString += "The rarefaction.single command should be in the following format: \n";
@@ -63,6 +64,7 @@ string RareFactCommand::getHelpString(){
                helpString += "Example rarefaction.single(label=unique-.01-.03, iters=10000, freq=10, calc=sobs-rchao-race-rjack-rbootstrap-rshannon-rnpshannon-rsimpson).\n";
                helpString += "The default values for iters is 1000, freq is 100, and calc is rarefaction which calculates the rarefaction curve for the observed richness.\n";
                validCalculator.printCalc("rarefaction");
+               helpString += "If you are running rarefaction.single with a shared file and would like your results collated in one file, set groupmode=t. (Default=true).\n";
                helpString += "The label parameter is used to analyze specific labels in your input.\n";
                helpString += "Note: No spaces between parameter labels (i.e. freq), '=' and parameters (i.e.yourFreq).\n";
                return helpString;
@@ -72,7 +74,34 @@ string RareFactCommand::getHelpString(){
                exit(1);
        }
 }
-
+//**********************************************************************************************************************
+string RareFactCommand::getOutputPattern(string type) {
+    try {
+        string pattern = "";
+        if (type == "rarefaction") {  pattern =  "[filename],rarefaction"; }
+        else if (type == "r_chao") {  pattern =  "[filename],r_chao"; }
+        else if (type == "r_ace") {  pattern =  "[filename],r_ace"; }
+        else if (type == "r_jack") {  pattern =  "[filename],r_jack"; }
+        else if (type == "r_shannon") {  pattern =  "[filename],r_shannon"; }
+        else if (type == "r_shannoneven") {  pattern =  "[filename],r_shannoneven"; }
+        else if (type == "r_smithwilson") {  pattern =  "[filename],r_smithwilson"; }
+        else if (type == "r_npshannon") {  pattern =  "[filename],r_npshannon"; }
+        else if (type == "r_simpson") {  pattern =  "[filename],r_simpson"; }
+        else if (type == "r_simpsoneven") {  pattern =  "[filename],r_simpsoneven"; }
+        else if (type == "r_invsimpson") {  pattern =  "[filename],r_invsimpson"; }
+        else if (type == "r_bootstrap") {  pattern =  "[filename],r_bootstrap"; }
+        else if (type == "r_coverage") {  pattern =  "[filename],r_coverage"; }
+        else if (type == "r_nseqs") {  pattern =  "[filename],r_nseqs"; }
+        else if (type == "r_heip") {  pattern =  "[filename],r_heip"; }
+        else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->control_pressed = true;  }
+        
+        return pattern;
+    }
+    catch(exception& e) {
+        m->errorOut(e, "RareFactCommand", "getOutputPattern");
+        exit(1);
+    }
+}
 //**********************************************************************************************************************
 RareFactCommand::RareFactCommand(){    
        try {
@@ -184,22 +213,22 @@ RareFactCommand::RareFactCommand(string option)  {
                        listfile = validParameter.validFile(parameters, "list", true);
                        if (listfile == "not open") { listfile = ""; abort = true; }
                        else if (listfile == "not found") { listfile = ""; }
-                       else {  format = "list"; inputfile = listfile; }
+                       else {  format = "list"; inputfile = listfile; m->setListFile(listfile); }
                        
                        sabundfile = validParameter.validFile(parameters, "sabund", true);
                        if (sabundfile == "not open") { sabundfile = ""; abort = true; }        
                        else if (sabundfile == "not found") { sabundfile = ""; }
-                       else {  format = "sabund"; inputfile = sabundfile; }
+                       else {  format = "sabund"; inputfile = sabundfile; m->setSabundFile(sabundfile); }
                        
                        rabundfile = validParameter.validFile(parameters, "rabund", true);
                        if (rabundfile == "not open") { rabundfile = ""; abort = true; }        
                        else if (rabundfile == "not found") { rabundfile = ""; }
-                       else {  format = "rabund"; inputfile = rabundfile; }
+                       else {  format = "rabund"; inputfile = rabundfile; m->setRabundFile(rabundfile); }
                        
                        sharedfile = validParameter.validFile(parameters, "shared", true);
                        if (sharedfile == "not open") { sharedfile = ""; abort = true; }        
                        else if (sharedfile == "not found") { sharedfile = ""; }
-                       else {  format = "sharedfile"; inputfile = sharedfile; }
+                       else {  format = "sharedfile"; inputfile = sharedfile; m->setSharedFile(sharedfile); }
                                
                        if ((sharedfile == "") && (listfile == "") && (rabundfile == "") && (sabundfile == "")) { 
                                //is there are current file available for any of these?
@@ -251,17 +280,20 @@ RareFactCommand::RareFactCommand(string option)  {
 
                        string temp;
                        temp = validParameter.validFile(parameters, "freq", false);                     if (temp == "not found") { temp = "100"; }
-                       convert(temp, freq); 
+                       m->mothurConvert(temp, freq); 
                        
                        temp = validParameter.validFile(parameters, "abund", false);                    if (temp == "not found") { temp = "10"; }
-                       convert(temp, abund); 
+                       m->mothurConvert(temp, abund); 
                        
                        temp = validParameter.validFile(parameters, "iters", false);                    if (temp == "not found") { temp = "1000"; }
-                       convert(temp, nIters); 
+                       m->mothurConvert(temp, nIters); 
                        
                        temp = validParameter.validFile(parameters, "processors", false);       if (temp == "not found"){       temp = m->getProcessors();      }
                        m->setProcessors(temp);
-                       convert(temp, processors);
+                       m->mothurConvert(temp, processors);
+                       
+                       temp = validParameter.validFile(parameters, "groupmode", false);                if (temp == "not found") { temp = "T"; }
+                       groupMode = m->isTrue(temp);
                }
                
        }
@@ -277,16 +309,18 @@ int RareFactCommand::execute(){
        
                if (abort == true) { if (calledHelp) { return 0; }  return 2;   }
                
+        map<string, set<int> > labelToEnds;
                if ((format != "sharedfile")) { inputFileNames.push_back(inputfile);  }
-               else {  inputFileNames = parseSharedFile(sharedfile);  format = "rabund"; }
-                               
-               if (m->control_pressed) { return 0; }
+               else {  inputFileNames = parseSharedFile(sharedfile, labelToEnds);  format = "rabund"; }
+        
+        if (m->control_pressed) { return 0; }
                
+               map<int, string> file2Group; //index in outputNames[i] -> group
                for (int p = 0; p < inputFileNames.size(); p++) {
                        
                        string fileNameRoot = outputDir + m->getRootName(m->getSimpleName(inputFileNames[p]));
                                                
-                       if (m->control_pressed) {  outputTypes.clear(); for (int i = 0; i < outputNames.size(); i++) {  remove(outputNames[i].c_str());         }  m->Groups.clear();  return 0; }
+                       if (m->control_pressed) {  outputTypes.clear(); for (int i = 0; i < outputNames.size(); i++) {  m->mothurRemove(outputNames[i]);        }  m->clearGroups();  return 0; }
                        
                        if (inputFileNames.size() > 1) {
                                m->mothurOutEndLine(); m->mothurOut("Processing group " + groups[p]); m->mothurOutEndLine(); m->mothurOutEndLine();
@@ -294,57 +328,60 @@ int RareFactCommand::execute(){
                        int i;
                        ValidCalculators validCalculator;
                        
+            map<string, string> variables; 
+            variables["[filename]"] = fileNameRoot;
                          
                        for (i=0; i<Estimators.size(); i++) {
                                if (validCalculator.isValidCalculator("rarefaction", Estimators[i]) == true) { 
                                        if (Estimators[i] == "sobs") { 
-                                               rDisplays.push_back(new RareDisplay(new Sobs(), new ThreeColumnFile(fileNameRoot+"rarefaction")));
-                                               outputNames.push_back(fileNameRoot+"rarefaction"); outputTypes["rarefaction"].push_back(fileNameRoot+"rarefaction");
+                                               rDisplays.push_back(new RareDisplay(new Sobs(), new ThreeColumnFile(getOutputFileName("rarefaction",variables))));
+                                               outputNames.push_back(getOutputFileName("rarefaction",variables)); outputTypes["rarefaction"].push_back(getOutputFileName("rarefaction",variables));
                                        }else if (Estimators[i] == "chao") { 
-                                               rDisplays.push_back(new RareDisplay(new Chao1(), new ThreeColumnFile(fileNameRoot+"r_chao")));
-                                               outputNames.push_back(fileNameRoot+"r_chao"); outputTypes["r_chao"].push_back(fileNameRoot+"r_chao");
+                                               rDisplays.push_back(new RareDisplay(new Chao1(), new ThreeColumnFile(getOutputFileName("r_chao",variables))));
+                                               outputNames.push_back(getOutputFileName("r_chao",variables)); outputTypes["r_chao"].push_back(getOutputFileName("r_chao",variables));
                                        }else if (Estimators[i] == "ace") { 
                                                if(abund < 5)
                                                        abund = 10;
-                                               rDisplays.push_back(new RareDisplay(new Ace(abund), new ThreeColumnFile(fileNameRoot+"r_ace")));
-                                               outputNames.push_back(fileNameRoot+"r_ace"); outputTypes["r_ace"].push_back(fileNameRoot+"r_ace");
+                                               rDisplays.push_back(new RareDisplay(new Ace(abund), new ThreeColumnFile(getOutputFileName("r_ace",variables))));
+                                               outputNames.push_back(getOutputFileName("r_ace",variables)); outputTypes["r_ace"].push_back(getOutputFileName("r_ace",variables));
                                        }else if (Estimators[i] == "jack") { 
-                                               rDisplays.push_back(new RareDisplay(new Jackknife(), new ThreeColumnFile(fileNameRoot+"r_jack")));
-                                               outputNames.push_back(fileNameRoot+"r_jack"); outputTypes["r_jack"].push_back(fileNameRoot+"r_jack");
+                                               rDisplays.push_back(new RareDisplay(new Jackknife(), new ThreeColumnFile(getOutputFileName("r_jack",variables))));
+                                               outputNames.push_back(getOutputFileName("r_jack",variables)); outputTypes["r_jack"].push_back(getOutputFileName("r_jack",variables));
                                        }else if (Estimators[i] == "shannon") { 
-                                               rDisplays.push_back(new RareDisplay(new Shannon(), new ThreeColumnFile(fileNameRoot+"r_shannon")));
-                                               outputNames.push_back(fileNameRoot+"r_shannon"); outputTypes["r_shannon"].push_back(fileNameRoot+"r_shannon");
+                                               rDisplays.push_back(new RareDisplay(new Shannon(), new ThreeColumnFile(getOutputFileName("r_shannon",variables))));
+                                               outputNames.push_back(getOutputFileName("r_shannon",variables)); outputTypes["r_shannon"].push_back(getOutputFileName("r_shannon",variables));
                                        }else if (Estimators[i] == "shannoneven") { 
-                                               rDisplays.push_back(new RareDisplay(new ShannonEven(), new ThreeColumnFile(fileNameRoot+"r_shannoneven")));
-                                               outputNames.push_back(fileNameRoot+"r_shannoneven"); outputTypes["r_shannoneven"].push_back(fileNameRoot+"r_shannoneven");
+                                               rDisplays.push_back(new RareDisplay(new ShannonEven(), new ThreeColumnFile(getOutputFileName("r_shannoneven",variables))));
+                                               outputNames.push_back(getOutputFileName("r_shannoneven",variables)); outputTypes["r_shannoneven"].push_back(getOutputFileName("r_shannoneven",variables));
                                        }else if (Estimators[i] == "heip") { 
-                                               rDisplays.push_back(new RareDisplay(new Heip(), new ThreeColumnFile(fileNameRoot+"r_heip")));
-                                               outputNames.push_back(fileNameRoot+"r_heip"); outputTypes["r_heip"].push_back(fileNameRoot+"r_heip");
+                                               rDisplays.push_back(new RareDisplay(new Heip(), new ThreeColumnFile(getOutputFileName("r_heip",variables))));
+                                               outputNames.push_back(getOutputFileName("r_heip",variables)); outputTypes["r_heip"].push_back(getOutputFileName("r_heip",variables));
                                        }else if (Estimators[i] == "smithwilson") { 
-                                               rDisplays.push_back(new RareDisplay(new SmithWilson(), new ThreeColumnFile(fileNameRoot+"r_smithwilson")));
-                                               outputNames.push_back(fileNameRoot+"r_smithwilson"); outputTypes["r_smithwilson"].push_back(fileNameRoot+"r_smithwilson");
+                                               rDisplays.push_back(new RareDisplay(new SmithWilson(), new ThreeColumnFile(getOutputFileName("r_smithwilson",variables))));
+                                               outputNames.push_back(getOutputFileName("r_smithwilson",variables)); outputTypes["r_smithwilson"].push_back(getOutputFileName("r_smithwilson",variables));
                                        }else if (Estimators[i] == "npshannon") { 
-                                               rDisplays.push_back(new RareDisplay(new NPShannon(), new ThreeColumnFile(fileNameRoot+"r_npshannon")));
-                                               outputNames.push_back(fileNameRoot+"r_npshannon"); outputTypes["r_npshannon"].push_back(fileNameRoot+"r_npshannon");
+                                               rDisplays.push_back(new RareDisplay(new NPShannon(), new ThreeColumnFile(getOutputFileName("r_npshannon",variables))));
+                                               outputNames.push_back(getOutputFileName("r_npshannon",variables)); outputTypes["r_npshannon"].push_back(getOutputFileName("r_npshannon",variables));
                                        }else if (Estimators[i] == "simpson") { 
-                                               rDisplays.push_back(new RareDisplay(new Simpson(), new ThreeColumnFile(fileNameRoot+"r_simpson")));
-                                               outputNames.push_back(fileNameRoot+"r_simpson"); outputTypes["r_simpson"].push_back(fileNameRoot+"r_simpson");
+                                               rDisplays.push_back(new RareDisplay(new Simpson(), new ThreeColumnFile(getOutputFileName("r_simpson",variables))));
+                                               outputNames.push_back(getOutputFileName("r_simpson",variables)); outputTypes["r_simpson"].push_back(getOutputFileName("r_simpson",variables));
                                        }else if (Estimators[i] == "simpsoneven") { 
-                                               rDisplays.push_back(new RareDisplay(new SimpsonEven(), new ThreeColumnFile(fileNameRoot+"r_simpsoneven")));
-                                               outputNames.push_back(fileNameRoot+"r_simpsoneven"); outputTypes["r_simpsoneven"].push_back(fileNameRoot+"r_simpsoneven");
+                                               rDisplays.push_back(new RareDisplay(new SimpsonEven(), new ThreeColumnFile(getOutputFileName("r_simpsoneven",variables))));
+                                               outputNames.push_back(getOutputFileName("r_simpsoneven",variables)); outputTypes["r_simpsoneven"].push_back(getOutputFileName("r_simpsoneven",variables));
                                        }else if (Estimators[i] == "invsimpson") { 
-                                               rDisplays.push_back(new RareDisplay(new InvSimpson(), new ThreeColumnFile(fileNameRoot+"r_invsimpson")));
-                                               outputNames.push_back(fileNameRoot+"r_invsimpson"); outputTypes["r_invsimpson"].push_back(fileNameRoot+"r_invsimpson");
+                                               rDisplays.push_back(new RareDisplay(new InvSimpson(), new ThreeColumnFile(getOutputFileName("r_invsimpson",variables))));
+                                               outputNames.push_back(getOutputFileName("r_invsimpson",variables)); outputTypes["r_invsimpson"].push_back(getOutputFileName("r_invsimpson",variables));
                                        }else if (Estimators[i] == "bootstrap") { 
-                                               rDisplays.push_back(new RareDisplay(new Bootstrap(), new ThreeColumnFile(fileNameRoot+"r_bootstrap")));
-                                               outputNames.push_back(fileNameRoot+"r_bootstrap"); outputTypes["r_bootstrap"].push_back(fileNameRoot+"r_bootstrap");
+                                               rDisplays.push_back(new RareDisplay(new Bootstrap(), new ThreeColumnFile(getOutputFileName("r_bootstrap",variables))));
+                                               outputNames.push_back(getOutputFileName("r_bootstrap",variables)); outputTypes["r_bootstrap"].push_back(getOutputFileName("r_bootstrap",variables));
                                        }else if (Estimators[i] == "coverage") { 
-                                               rDisplays.push_back(new RareDisplay(new Coverage(), new ThreeColumnFile(fileNameRoot+"r_coverage")));
-                                               outputNames.push_back(fileNameRoot+"r_coverage"); outputTypes["r_coverage"].push_back(fileNameRoot+"r_coverage");
+                                               rDisplays.push_back(new RareDisplay(new Coverage(), new ThreeColumnFile(getOutputFileName("r_coverage",variables))));
+                                               outputNames.push_back(getOutputFileName("r_coverage",variables)); outputTypes["r_coverage"].push_back(getOutputFileName("r_coverage",variables));
                                        }else if (Estimators[i] == "nseqs") { 
-                                               rDisplays.push_back(new RareDisplay(new NSeqs(), new ThreeColumnFile(fileNameRoot+"r_nseqs")));
-                                               outputNames.push_back(fileNameRoot+"r_nseqs"); outputTypes["r_nseqs"].push_back(fileNameRoot+"r_nseqs");
+                                               rDisplays.push_back(new RareDisplay(new NSeqs(), new ThreeColumnFile(getOutputFileName("r_nseqs",variables))));
+                                               outputNames.push_back(getOutputFileName("r_nseqs",variables)); outputTypes["r_nseqs"].push_back(getOutputFileName("r_nseqs",variables));
                                        }
+                    if (inputFileNames.size() > 1) { file2Group[outputNames.size()-1] = groups[p]; }
                                }
                        }
                        
@@ -360,18 +397,21 @@ int RareFactCommand::execute(){
                        set<string> processedLabels;
                        set<string> userLabels = labels;
                        
-                       if (m->control_pressed) { for(int i=0;i<rDisplays.size();i++){  delete rDisplays[i];    }  delete input;  delete order;  for (int i = 0; i < outputNames.size(); i++) { remove(outputNames[i].c_str()); } return 0; }
+                       if (m->control_pressed) { for(int i=0;i<rDisplays.size();i++){  delete rDisplays[i];    }  delete input;  delete order;  for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; }
                        
                        //as long as you are not at the end of the file or done wih the lines you want
                        while((order != NULL) && ((allLines == 1) || (userLabels.size() != 0))) {
                                
-                               if (m->control_pressed) { for(int i=0;i<rDisplays.size();i++){  delete rDisplays[i];    }  delete input;  delete order;  for (int i = 0; i < outputNames.size(); i++) { remove(outputNames[i].c_str()); } return 0; }
+                               if (m->control_pressed) { for(int i=0;i<rDisplays.size();i++){  delete rDisplays[i];    }  delete input;  delete order;  for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; }
 
                                
                                if(allLines == 1 || labels.count(order->getLabel()) == 1){
                                        
                                        m->mothurOut(order->getLabel()); m->mothurOutEndLine();
-                                       rCurve = new Rarefact(order, rDisplays, processors);
+                    map<string, set<int> >::iterator itEndings = labelToEnds.find(order->getLabel());
+                    set<int> ends;
+                    if (itEndings != labelToEnds.end()) { ends = itEndings->second; }
+                                       rCurve = new Rarefact(order, rDisplays, processors, ends);
                                        rCurve->getCurve(freq, nIters);
                                        delete rCurve;
                                        
@@ -386,7 +426,11 @@ int RareFactCommand::execute(){
                                        order = (input->getOrderVector(lastLabel));
                                        
                                        m->mothurOut(order->getLabel()); m->mothurOutEndLine();
-                                       rCurve = new Rarefact(order, rDisplays, processors);
+                                       map<string, set<int> >::iterator itEndings = labelToEnds.find(order->getLabel());
+                    set<int> ends;
+                    if (itEndings != labelToEnds.end()) { ends = itEndings->second; }
+                                       rCurve = new Rarefact(order, rDisplays, processors, ends);
+
                                        rCurve->getCurve(freq, nIters);
                                        delete rCurve;
                                        
@@ -403,7 +447,7 @@ int RareFactCommand::execute(){
                                order = (input->getOrderVector());
                        }
                        
-                       if (m->control_pressed) { for(int i=0;i<rDisplays.size();i++){  delete rDisplays[i];    }  delete input;   for (int i = 0; i < outputNames.size(); i++) {       remove(outputNames[i].c_str()); } return 0; }
+                       if (m->control_pressed) { for(int i=0;i<rDisplays.size();i++){  delete rDisplays[i];    }  delete input;   for (int i = 0; i < outputNames.size(); i++) {       m->mothurRemove(outputNames[i]); } return 0; }
 
                        //output error messages about any remaining user labels
                        set<string>::iterator it;
@@ -418,7 +462,7 @@ int RareFactCommand::execute(){
                                }
                        }
                        
-                       if (m->control_pressed) { for(int i=0;i<rDisplays.size();i++){  delete rDisplays[i];    }  delete input;   for (int i = 0; i < outputNames.size(); i++) {       remove(outputNames[i].c_str()); } return 0; }
+                       if (m->control_pressed) { for(int i=0;i<rDisplays.size();i++){  delete rDisplays[i];    }  delete input;   for (int i = 0; i < outputNames.size(); i++) {       m->mothurRemove(outputNames[i]); } return 0; }
 
                        //run last label if you need to
                        if (needToRun == true)  {
@@ -426,7 +470,11 @@ int RareFactCommand::execute(){
                                order = (input->getOrderVector(lastLabel));
                                
                                m->mothurOut(order->getLabel()); m->mothurOutEndLine();
-                               rCurve = new Rarefact(order, rDisplays, processors);
+                               map<string, set<int> >::iterator itEndings = labelToEnds.find(order->getLabel());
+                set<int> ends;
+                if (itEndings != labelToEnds.end()) { ends = itEndings->second; }
+                rCurve = new Rarefact(order, rDisplays, processors, ends);
+
                                rCurve->getCurve(freq, nIters);
                                delete rCurve;
                                
@@ -440,7 +488,12 @@ int RareFactCommand::execute(){
                }
                
                
-               if (m->control_pressed) {  for (int i = 0; i < outputNames.size(); i++) {       remove(outputNames[i].c_str()); } return 0; }
+               if (m->control_pressed) {  for (int i = 0; i < outputNames.size(); i++) {       m->mothurRemove(outputNames[i]); } return 0; }
+
+               //create summary file containing all the groups data for each label - this function just combines the info from the files already created.
+               if ((sharedfile != "") && (groupMode)) {   outputNames = createGroupFile(outputNames, file2Group);  }
+
+               if (m->control_pressed) {  for (int i = 0; i < outputNames.size(); i++) {       m->mothurRemove(outputNames[i]); } return 0; }
 
                m->mothurOutEndLine();
                m->mothurOut("Output File Names: "); m->mothurOutEndLine();
@@ -455,7 +508,165 @@ int RareFactCommand::execute(){
        }
 }
 //**********************************************************************************************************************
-vector<string> RareFactCommand::parseSharedFile(string filename) {
+vector<string> RareFactCommand::createGroupFile(vector<string>& outputNames, map<int, string> file2Group) {
+       try {
+               
+               vector<string> newFileNames;
+               
+               //find different types of files
+               map<string, map<string, string> > typesFiles;
+        map<string, vector< vector<string> > > fileLabels; //combofile name to labels. each label is a vector because it may be unique lci hci.
+        vector<string> groupNames;
+               for (int i = 0; i < outputNames.size(); i++) {
+            
+                       string extension = m->getExtension(outputNames[i]);
+            string combineFileName = outputDir + m->getRootName(m->getSimpleName(sharedfile)) + "groups" + extension;
+                       m->mothurRemove(combineFileName); //remove old file
+            
+                       ifstream in;
+                       m->openInputFile(outputNames[i], in);
+                       
+                       string labels = m->getline(in);
+            
+                       istringstream iss (labels,istringstream::in);
+            string newLabel = ""; vector<string> theseLabels;
+            while(!iss.eof()) {  iss >> newLabel; m->gobble(iss); theseLabels.push_back(newLabel); }
+            vector< vector<string> > allLabels;
+            vector<string> thisSet; thisSet.push_back(theseLabels[0]); allLabels.push_back(thisSet); thisSet.clear(); //makes "numSampled" its own grouping
+            for (int j = 1; j < theseLabels.size()-1; j++) {
+                if (theseLabels[j+1] == "lci") {
+                    thisSet.push_back(theseLabels[j]); 
+                    thisSet.push_back(theseLabels[j+1]); 
+                    thisSet.push_back(theseLabels[j+2]);
+                    j++; j++;
+                }else{ //no lci or hci for this calc.
+                    thisSet.push_back(theseLabels[j]); 
+                }
+                allLabels.push_back(thisSet); 
+                thisSet.clear();
+            }
+            fileLabels[combineFileName] = allLabels;
+                    
+            map<string, map<string, string> >::iterator itfind = typesFiles.find(extension);
+            if (itfind != typesFiles.end()) {
+                (itfind->second)[outputNames[i]] = file2Group[i];
+            }else {
+                map<string, string> temp;  
+                temp[outputNames[i]] = file2Group[i];
+                typesFiles[extension] = temp;
+            }
+            if (!(m->inUsersGroups(file2Group[i], groupNames))) {  groupNames.push_back(file2Group[i]); }
+               }
+               
+               //for each type create a combo file
+               
+               for (map<string, map<string, string> >::iterator it = typesFiles.begin(); it != typesFiles.end(); it++) {
+                       
+                       ofstream out;
+                       string combineFileName = outputDir + m->getRootName(m->getSimpleName(sharedfile)) + "groups" + it->first;
+                       m->openOutputFileAppend(combineFileName, out);
+                       newFileNames.push_back(combineFileName);
+                       map<string, string> thisTypesFiles = it->second; //it->second maps filename to group
+            set<int> numSampledSet;
+            
+                       //open each type summary file
+                       map<string, map<int, vector< vector<string> > > > files; //maps file name to lines in file
+                       int maxLines = 0;
+                       for (map<string, string>::iterator itFileNameGroup = thisTypesFiles.begin(); itFileNameGroup != thisTypesFiles.end(); itFileNameGroup++) {
+                
+                string thisfilename = itFileNameGroup->first;
+                string group = itFileNameGroup->second;
+                
+                               ifstream temp;
+                               m->openInputFile(thisfilename, temp);
+                               
+                               //read through first line - labels
+                               m->getline(temp);       m->gobble(temp);
+                               
+                               map<int, vector< vector<string> > > thisFilesLines;
+                               while (!temp.eof()){
+                    int numSampled = 0;
+                    temp >> numSampled; m->gobble(temp);
+                
+                    vector< vector<string> > theseReads;
+                    vector<string> thisSet; thisSet.push_back(toString(numSampled)); theseReads.push_back(thisSet); thisSet.clear();
+                    for (int k = 1; k < fileLabels[combineFileName].size(); k++) { //output thing like 0.03-A lci-A hci-A
+                        vector<string> reads;
+                        string next = "";
+                        for (int l = 0; l < fileLabels[combineFileName][k].size(); l++) { //output modified labels
+                            temp >> next; m->gobble(temp);
+                            reads.push_back(next);
+                        }
+                        theseReads.push_back(reads);
+                    }
+                    thisFilesLines[numSampled] = theseReads;
+                    m->gobble(temp);
+                   
+                    numSampledSet.insert(numSampled);
+                               }
+                               
+                               files[group] = thisFilesLines;
+                               
+                               //save longest file for below
+                               if (maxLines < thisFilesLines.size()) { maxLines = thisFilesLines.size(); }
+                               
+                               temp.close();
+                               m->mothurRemove(thisfilename);
+                       }
+                       
+            //output new labels line
+            out << fileLabels[combineFileName][0][0] << '\t';
+            for (int k = 1; k < fileLabels[combineFileName].size(); k++) { //output thing like 0.03-A lci-A hci-A
+                for (int n = 0; n < groupNames.size(); n++) { // for each group
+                    for (int l = 0; l < fileLabels[combineFileName][k].size(); l++) { //output modified labels
+                        out << fileLabels[combineFileName][k][l] << '-' << groupNames[n] << '\t';
+                    }
+                }
+            }
+                       out << endl;
+            
+                       //for each label
+                       for (set<int>::iterator itNumSampled = numSampledSet.begin(); itNumSampled != numSampledSet.end(); itNumSampled++) {
+                               
+                out << (*itNumSampled) << '\t';
+                               
+                if (m->control_pressed) { break; }
+                
+                for (int k = 1; k < fileLabels[combineFileName].size(); k++) { //each chunk
+                                   //grab data for each group
+                    for (map<string, map<int, vector< vector<string> > > >::iterator itFileNameGroup = files.begin(); itFileNameGroup != files.end(); itFileNameGroup++) {
+                        
+                        string group = itFileNameGroup->first;
+                       
+                        map<int, vector< vector<string> > >::iterator itLine = files[group].find(*itNumSampled);
+                        if (itLine != files[group].end()) { 
+                            for (int l = 0; l < (itLine->second)[k].size(); l++) { 
+                                out << (itLine->second)[k][l] << '\t';
+                               
+                            }                             
+                        }else { 
+                            for (int l = 0; l < fileLabels[combineFileName][k].size(); l++) { 
+                                out << "NA" << '\t';
+                            } 
+                        }
+                    }
+                }
+                out << endl;
+                       }       
+                       out.close();
+               }
+               
+               //return combine file name
+               return newFileNames;
+               
+       }
+       catch(exception& e) {
+               m->errorOut(e, "RareFactCommand", "createGroupFile");
+               exit(1);
+       }
+}
+//**********************************************************************************************************************
+vector<string> RareFactCommand::parseSharedFile(string filename, map<string, set<int> >& label2Ends) {
        try {
                vector<string> filenames;
                
@@ -469,7 +680,7 @@ vector<string> RareFactCommand::parseSharedFile(string filename) {
                
                //clears file before we start to write to it below
                for (int i=0; i<lookup.size(); i++) {
-                       remove((sharedFileRoot + lookup[i]->getGroup() + ".rabund").c_str());
+                       m->mothurRemove((sharedFileRoot + lookup[i]->getGroup() + ".rabund"));
                        filenames.push_back((sharedFileRoot + lookup[i]->getGroup() + ".rabund"));
                }
                
@@ -487,6 +698,7 @@ vector<string> RareFactCommand::parseSharedFile(string filename) {
                                m->openOutputFileAppend(sharedFileRoot + lookup[i]->getGroup() + ".rabund", *(filehandles[lookup[i]->getGroup()]));
                                rav.print(*(filehandles[lookup[i]->getGroup()]));
                                (*(filehandles[lookup[i]->getGroup()])).close();
+                label2Ends[lookup[i]->getLabel()].insert(rav.getNumSeqs());
                        }
                
                        for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } 
@@ -499,7 +711,7 @@ vector<string> RareFactCommand::parseSharedFile(string filename) {
                }
                
                delete input;
-               m->Groups.clear();
+               m->clearGroups();
 
                return filenames;
        }