if (numSeqs == 0) { m->mothurOut("Error reading fasta file...please correct."); m->mothurOutEndLine(); return 0; }
if (diffs > length) { m->mothurOut("Error: diffs is greater than your sequence length."); m->mothurOutEndLine(); return 0; }
- string fileroot = outputDir + m->getRootName(m->getSimpleName(fastafile));
- string newFastaFile = fileroot + "precluster" + m->getExtension(fastafile);
- string newNamesFile = fileroot + "precluster.names";
- ofstream outFasta;
- ofstream outNames;
-
- m->openOutputFile(newFastaFile, outFasta);
- m->openOutputFile(newNamesFile, outNames);
-
+ //clear sizes since you only needed this info to build the alignSeqs seqPNode structs
+// sizes.clear();
+
//sort seqs by number of identical seqs
- alignSeqs.sort(comparePriority);
-
+ sort(alignSeqs.begin(), alignSeqs.end(), comparePriority);
+
int count = 0;
- int i = 0;
+
//think about running through twice...
- list<seqPNode>::iterator itList;
- list<seqPNode>::iterator itList2;
- for (itList = alignSeqs.begin(); itList != alignSeqs.end();) {
+ for (int i = 0; i < numSeqs; i++) {
- //try to merge it with all smaller seqs
- for (itList2 = alignSeqs.begin(); itList2 != alignSeqs.end();) {
-
- if (m->control_pressed) { outFasta.close(); outNames.close(); remove(newFastaFile.c_str()); remove(newNamesFile.c_str()); return 0; }
-
+ //are you active
+ // itActive = active.find(alignSeqs[i].seq.getName());
- if (itList->seq.getName() != itList2->seq.getName()) { //you don't want to merge with yourself
- //are you within "diff" bases
+ if (alignSeqs[i].active) { //this sequence has not been merged yet
+
+ //try to merge it with all smaller seqs
+ for (int j = i+1; j < numSeqs; j++) {
- int mismatch = calcMisMatches((*itList).seq.getAligned(), (*itList2).seq.getAligned());
-
- if (mismatch <= diffs) {
- //merge
- (*itList).names += ',' + (*itList2).names;
- (*itList).numIdentical += (*itList2).numIdentical;
+ if (m->control_pressed) { return 0; }
+
+ if (alignSeqs[j].active) { //this sequence has not been merged yet
+ //are you within "diff" bases
+ int mismatch = calcMisMatches(alignSeqs[i].seq.getAligned(), alignSeqs[j].seq.getAligned());
- itList2 = alignSeqs.erase(itList2); //itList2--;
- count++;
- }else{ itList2++; }
- }else{ itList2++; }
-
- }
+ if (mismatch <= diffs) {
+ //merge
+ alignSeqs[i].names += ',' + alignSeqs[j].names;
+ alignSeqs[i].numIdentical += alignSeqs[j].numIdentical;
- //ouptut this sequence
- printData(outFasta, outNames, (*itList));
-
- //remove sequence
- itList = alignSeqs.erase(itList);
-
- i++;
+ alignSeqs[j].active = 0;
+ alignSeqs[j].numIdentical = 0;
+ count++;
+ }
+ }//end if j active
+ }//end if i != j
+ //remove from active list
+ alignSeqs[i].active = 0;
+
+ }//end if active i
if(i % 100 == 0) { m->mothurOut(toString(i) + "\t" + toString(numSeqs - count) + "\t" + toString(count)); m->mothurOutEndLine(); }
}
-
- if(i % 100 != 0) { m->mothurOut(toString(i) + "\t" + toString(numSeqs - count) + "\t" + toString(count)); m->mothurOutEndLine(); }
- outFasta.close();
- outNames.close();
+ if(numSeqs % 100 != 0) { m->mothurOut(toString(numSeqs) + "\t" + toString(numSeqs - count) + "\t" + toString(count)); m->mothurOutEndLine(); }
+
- if (m->control_pressed) { remove(newFastaFile.c_str()); remove(newNamesFile.c_str()); return 0; }
-
- m->mothurOut("It took " + toString(time(NULL) - start) + " secs to cluster " + toString(numSeqs) + " sequences.");
- m->mothurOut("Total number of sequences before precluster was " + toString(numSeqs) + "."); m->mothurOutEndLine();
- m->mothurOut("pre.cluster removed " + toString(count) + " sequences."); m->mothurOutEndLine();
+ string fileroot = outputDir + m->getRootName(m->getSimpleName(fastafile));
+
+ string newFastaFile = fileroot + "precluster" + m->getExtension(fastafile);
+ string newNamesFile = fileroot + "precluster.names";
+
+ if (m->control_pressed) { return 0; }
+
+ m->mothurOut("Total number of sequences before precluster was " + toString(alignSeqs.size()) + "."); m->mothurOutEndLine();
+ m->mothurOut("pre.cluster removed " + toString(count) + " sequences."); m->mothurOutEndLine(); m->mothurOutEndLine();
+ printData(newFastaFile, newNamesFile);
+
+ m->mothurOut("It took " + toString(time(NULL) - start) + " secs to cluster " + toString(numSeqs) + " sequences."); m->mothurOutEndLine();
if (m->control_pressed) { remove(newFastaFile.c_str()); remove(newNamesFile.c_str()); return 0; }
/**************************************************************************************************/
-void PreClusterCommand::printData(ofstream& outFasta, ofstream& outNames, seqPNode thisSeq){
+void PreClusterCommand::printData(string newfasta, string newname){
try {
- thisSeq.seq.printSequence(outFasta);
- outNames << thisSeq.seq.getName() << '\t' << thisSeq.names << endl;
+ ofstream outFasta;
+ ofstream outNames;
+
+ m->openOutputFile(newfasta, outFasta);
+ m->openOutputFile(newname, outNames);
+
+
+ for (int i = 0; i < alignSeqs.size(); i++) {
+ if (alignSeqs[i].numIdentical != 0) {
+ alignSeqs[i].seq.printSequence(outFasta);
+ outNames << alignSeqs[i].seq.getName() << '\t' << alignSeqs[i].names << endl;
+ }
+ }
+
+ outFasta.close();
+ outNames.close();
+
}
catch(exception& e) {
m->errorOut(e, "PreClusterCommand", "printData");
projects / mothur.git / blobdiff