]> git.donarmstrong.com Git - mothur.git/blobdiff - mgclustercommand.cpp
sffinfo bug with flow grams right index when clipQualRight=0
[mothur.git] / mgclustercommand.cpp
index 23f359eae8ab7fe84e9b86faf96d54d33ac8dcd3..24c4f27777d21ed88b6f3b41411394502802404e 100644 (file)
 
 #include "mgclustercommand.h"
 
-
 //**********************************************************************************************************************
-vector<string> MGClusterCommand::getValidParameters(){ 
+vector<string> MGClusterCommand::setParameters(){      
        try {
-               string Array[] =  {"blast", "method", "name", "hard", "cutoff", "precision", "length", "min", "penalty", "hcluster","merge","outputdir","inputdir"};
-               vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
+               CommandParameter pblast("blast", "InputTypes", "", "", "none", "none", "none","list",false,true,true); parameters.push_back(pblast);
+               CommandParameter pname("name", "InputTypes", "", "", "NameCount", "none", "ColumnName","rabund-sabund",false,false,true); parameters.push_back(pname);
+               CommandParameter pcount("count", "InputTypes", "", "", "NameCount", "none", "none","",false,false,true); parameters.push_back(pcount);
+               CommandParameter plength("length", "Number", "", "5", "", "", "","",false,false); parameters.push_back(plength);
+               CommandParameter ppenalty("penalty", "Number", "", "0.10", "", "", "","",false,false); parameters.push_back(ppenalty);
+               CommandParameter pcutoff("cutoff", "Number", "", "0.70", "", "", "","",false,false,true); parameters.push_back(pcutoff);
+               CommandParameter pprecision("precision", "Number", "", "100", "", "", "","",false,false); parameters.push_back(pprecision);
+               CommandParameter pmethod("method", "Multiple", "furthest-nearest-average", "average", "", "", "","",false,false); parameters.push_back(pmethod);
+               CommandParameter phard("hard", "Boolean", "", "T", "", "", "","",false,false); parameters.push_back(phard);
+               CommandParameter pmin("min", "Boolean", "", "T", "", "", "","",false,false); parameters.push_back(pmin);
+               CommandParameter pmerge("merge", "Boolean", "", "T", "", "", "","",false,false); parameters.push_back(pmerge);
+               CommandParameter phcluster("hcluster", "Boolean", "", "F", "", "", "","",false,false); parameters.push_back(phcluster);
+               CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
+               CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
+               
+               vector<string> myArray;
+               for (int i = 0; i < parameters.size(); i++) {   myArray.push_back(parameters[i].name);          }
                return myArray;
        }
        catch(exception& e) {
-               m->errorOut(e, "MGClusterCommand", "getValidParameters");
+               m->errorOut(e, "MGClusterCommand", "setParameters");
                exit(1);
        }
 }
 //**********************************************************************************************************************
-MGClusterCommand::MGClusterCommand(){  
+string MGClusterCommand::getHelpString(){      
        try {
-               abort = true; calledHelp = true; 
-               vector<string> tempOutNames;
-               outputTypes["list"] = tempOutNames;
-               outputTypes["rabund"] = tempOutNames;
-               outputTypes["sabund"] = tempOutNames;
+               string helpString = "";
+               helpString += "The mgcluster command parameter options are blast, name, cutoff, precision, hard,  method, merge, min, length, penalty and hcluster. The blast parameter is required.\n";
+               helpString += "The mgcluster command reads a blast and name file and clusters the sequences into OPF units similiar to the OTUs.\n";
+               helpString += "This command outputs a .list, .rabund and .sabund file that can be used with mothur other commands to estimate richness.\n";
+               helpString += "The cutoff parameter is used to specify the maximum distance you would like to cluster to. The default is 0.70.\n";
+               helpString += "The precision parameter's default value is 100. \n";
+               helpString += "The acceptable mgcluster methods are furthest, nearest and average.  If no method is provided then average is assumed.\n";       
+               helpString += "The min parameter allows you to specify is you want the minimum or maximum blast score ratio used in calculating the distance. The default is true, meaning you want the minimum.\n";
+               helpString += "The length parameter is used to specify the minimum overlap required.  The default is 5.\n";
+               helpString += "The penalty parameter is used to adjust the error rate.  The default is 0.10.\n";
+               helpString += "The merge parameter allows you to shut off merging based on overlaps and just cluster.  By default merge is true, meaning you want to merge.\n";
+               helpString += "The hcluster parameter allows you to use the hcluster algorithm when clustering.  This may be neccessary if your file is too large to fit into RAM. The default is false.\n";
+               helpString += "The mgcluster command should be in the following format: \n";
+               helpString += "mgcluster(blast=yourBlastfile, name=yourNameFile, cutoff=yourCutOff).\n";
+               helpString += "Note: No spaces between parameter labels (i.e. balst), '=' and parameters (i.e.yourBlastfile).\n";
+               return helpString;
        }
        catch(exception& e) {
-               m->errorOut(e, "MGClusterCommand", "MGClusterCommand");
+               m->errorOut(e, "MGClusterCommand", "getHelpString");
                exit(1);
        }
 }
 //**********************************************************************************************************************
-vector<string> MGClusterCommand::getRequiredParameters(){      
-       try {
-               string Array[] =  {"blast"};
-               vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
-               return myArray;
-       }
-       catch(exception& e) {
-               m->errorOut(e, "MGClusterCommand", "getRequiredParameters");
-               exit(1);
-       }
+string MGClusterCommand::getOutputPattern(string type) {
+    try {
+        string pattern = "";
+        
+        if (type == "list") {  pattern = "[filename],[clustertag],list-[filename],[clustertag],[tag2],list"; } 
+        else if (type == "rabund") {  pattern = "[filename],[clustertag],rabund"; } 
+        else if (type == "sabund") {  pattern = "[filename],[clustertag],sabund"; }
+        else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->control_pressed = true;  }
+        
+        return pattern;
+    }
+    catch(exception& e) {
+        m->errorOut(e, "MGClusterCommand", "getOutputPattern");
+        exit(1);
+    }
 }
-//**********************************************************************************************************************
-vector<string> MGClusterCommand::getRequiredFiles(){   
+//*******************************************************************************************************************
+MGClusterCommand::MGClusterCommand(){  
        try {
-               vector<string> myArray;
-               return myArray;
+               abort = true; calledHelp = true; 
+               setParameters();
+               vector<string> tempOutNames;
+               outputTypes["list"] = tempOutNames;
+               outputTypes["rabund"] = tempOutNames;
+               outputTypes["sabund"] = tempOutNames;
        }
        catch(exception& e) {
-               m->errorOut(e, "MGClusterCommand", "getRequiredFiles");
+               m->errorOut(e, "MGClusterCommand", "MGClusterCommand");
                exit(1);
        }
 }
 //**********************************************************************************************************************
 MGClusterCommand::MGClusterCommand(string option) {
        try {
-               globaldata = GlobalData::getInstance();
                abort = false; calledHelp = false;   
                
                //allow user to run help
                if(option == "help") { help(); abort = true; calledHelp = true; }
+               else if(option == "citation") { citation(); abort = true; calledHelp = true;}
                
                else {
-                       //valid paramters for this command
-                       string Array[] =  {"blast", "method", "name", "hard", "cutoff", "precision", "length", "min", "penalty", "hcluster","merge","outputdir","inputdir"};
-                       vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
+                       vector<string> myArray = setParameters();
                        
                        OptionParser parser(option);
                        map<string, string> parameters = parser.getParameters();
@@ -110,12 +142,20 @@ MGClusterCommand::MGClusterCommand(string option) {
                                        //if the user has not given a path then, add inputdir. else leave path alone.
                                        if (path == "") {       parameters["name"] = inputDir + it->second;             }
                                }
+                
+                it = parameters.find("count");
+                               //user has given a template file
+                               if(it != parameters.end()){ 
+                                       path = m->hasPath(it->second);
+                                       //if the user has not given a path then, add inputdir. else leave path alone.
+                                       if (path == "") {       parameters["count"] = inputDir + it->second;            }
+                               }
                        }
 
                        
                        //check for required parameters
                        blastfile = validParameter.validFile(parameters, "blast", true);
-                       if (blastfile == "not open") { abort = true; }  
+                       if (blastfile == "not open") { blastfile = ""; abort = true; }  
                        else if (blastfile == "not found") { blastfile = ""; }
                        
                        //if the user changes the output directory command factory will send this info to us in the output parameter 
@@ -127,30 +167,38 @@ MGClusterCommand::MGClusterCommand(string option) {
                        namefile = validParameter.validFile(parameters, "name", true);
                        if (namefile == "not open") { abort = true; }   
                        else if (namefile == "not found") { namefile = ""; }
+                       else { m->setNameFile(namefile); }
+            
+            countfile = validParameter.validFile(parameters, "count", true);
+                       if (countfile == "not open") { abort = true; }  
+                       else if (countfile == "not found") { countfile = ""; }
+            else { m->setCountTableFile(countfile); }
+            
+            if (countfile != "" && namefile != "") { m->mothurOut("[ERROR]: Cannot have both a name file and count file. Please use one or the other."); m->mothurOutEndLine(); abort = true; }
                        
                        if ((blastfile == "")) { m->mothurOut("When executing a mgcluster command you must provide a blastfile."); m->mothurOutEndLine(); abort = true; }
                        
                        //check for optional parameter and set defaults
                        string temp;
-                       temp = validParameter.validFile(parameters, "precision", false);                if (temp == "not found") { temp = "100"; }
+            temp = validParameter.validFile(parameters, "precision", false);           if (temp == "not found") { temp = "100"; }
                        precisionLength = temp.length();
-                       convert(temp, precision); 
+                       m->mothurConvert(temp, precision); 
                        
                        temp = validParameter.validFile(parameters, "cutoff", false);                   if (temp == "not found") { temp = "0.70"; }
-                       convert(temp, cutoff); 
+                       m->mothurConvert(temp, cutoff); 
                        cutoff += (5 / (precision * 10.0));
                        
                        method = validParameter.validFile(parameters, "method", false);
-                       if (method == "not found") { method = "furthest"; }
+                       if (method == "not found") { method = "average"; }
                        
                        if ((method == "furthest") || (method == "nearest") || (method == "average")) { }
                        else { m->mothurOut("Not a valid clustering method.  Valid clustering algorithms are furthest, nearest or average."); m->mothurOutEndLine(); abort = true; }
 
                        temp = validParameter.validFile(parameters, "length", false);                   if (temp == "not found") { temp = "5"; }
-                       convert(temp, length); 
+                       m->mothurConvert(temp, length); 
                        
                        temp = validParameter.validFile(parameters, "penalty", false);                  if (temp == "not found") { temp = "0.10"; }
-                       convert(temp, penalty); 
+                       m->mothurConvert(temp, penalty); 
                        
                        temp = validParameter.validFile(parameters, "min", false);                              if (temp == "not found") { temp = "true"; }
                        minWanted = m->isTrue(temp); 
@@ -161,8 +209,8 @@ MGClusterCommand::MGClusterCommand(string option) {
                        temp = validParameter.validFile(parameters, "hcluster", false);                 if (temp == "not found") { temp = "false"; }
                        hclusterWanted = m->isTrue(temp); 
                        
-                       temp = validParameter.validFile(parameters, "hard", false);                     if (temp == "not found") { temp = "F"; }
-                       hard = m->isTrue(temp);
+                       temp = validParameter.validFile(parameters, "hard", false);                     if (temp == "not found") { temp = "T"; }
+                       hard = m->isTrue(temp);            
                }
 
        }
@@ -172,35 +220,8 @@ MGClusterCommand::MGClusterCommand(string option) {
        }
 }
 //**********************************************************************************************************************
-
-void MGClusterCommand::help(){
-       try {
-               m->mothurOut("The mgcluster command parameter options are blast, name, cutoff, precision, method, merge, min, length, penalty and hcluster. The blast parameter is required.\n");
-               m->mothurOut("The mgcluster command reads a blast and name file and clusters the sequences into OPF units similiar to the OTUs.\n");
-               m->mothurOut("This command outputs a .list, .rabund and .sabund file that can be used with mothur other commands to estimate richness.\n");
-               m->mothurOut("The cutoff parameter is used to specify the maximum distance you would like to cluster to. The default is 0.70.\n");
-               m->mothurOut("The precision parameter's default value is 100. \n");
-               m->mothurOut("The acceptable mgcluster methods are furthest, nearest and average.  If no method is provided then furthest is assumed.\n\n");    
-               m->mothurOut("The min parameter allows you to specify is you want the minimum or maximum blast score ratio used in calculating the distance. The default is true, meaning you want the minimum.\n");
-               m->mothurOut("The length parameter is used to specify the minimum overlap required.  The default is 5.\n");
-               m->mothurOut("The penalty parameter is used to adjust the error rate.  The default is 0.10.\n");
-               m->mothurOut("The merge parameter allows you to shut off merging based on overlaps and just cluster.  By default merge is true, meaning you want to merge.\n");
-               m->mothurOut("The hcluster parameter allows you to use the hcluster algorithm when clustering.  This may be neccessary if your file is too large to fit into RAM. The default is false.\n");
-               m->mothurOut("The mgcluster command should be in the following format: \n");
-               m->mothurOut("mgcluster(blast=yourBlastfile, name=yourNameFile, cutoff=yourCutOff).\n");
-               m->mothurOut("Note: No spaces between parameter labels (i.e. balst), '=' and parameters (i.e.yourBlastfile).\n\n");
-       }
-       catch(exception& e) {
-               m->errorOut(e, "MGClusterCommand", "help");
-               exit(1);
-       }
-}
-//**********************************************************************************************************************
-MGClusterCommand::~MGClusterCommand(){}
-//**********************************************************************************************************************
 int MGClusterCommand::execute(){
        try {
-               
                if (abort == true) { if (calledHelp) { return 0; }  return 2;   }
                
                //read names file
@@ -213,15 +234,29 @@ int MGClusterCommand::execute(){
                string tag = "";
                time_t start;
                float previousDist = 0.00000;
-               float rndPreviousDist = 0.00000;
-               
+               float rndPreviousDist = 0.00000; 
+        
                //read blastfile - creates sparsematrices for the distances and overlaps as well as a listvector
                //must remember to delete those objects here since readBlast does not
                read = new ReadBlast(blastfile, cutoff, penalty, length, minWanted, hclusterWanted);
                read->read(nameMap);
-               
-               list = new ListVector(nameMap->getListVector());
-               RAbundVector* rabund = new RAbundVector(list->getRAbundVector());
+        
+        list = new ListVector(nameMap->getListVector());
+        RAbundVector* rabund = NULL;
+        
+        if(countfile != "") {
+            //map<string, int> nameMapCounts = m->readNames(namefile);
+            ct = new CountTable();
+            ct->readTable(countfile);
+            rabund = new RAbundVector();
+            createRabund(ct, list, rabund);
+        }else {
+            rabund = new RAbundVector(list->getRAbundVector());
+        }
+        
+                
+               //list = new ListVector(nameMap->getListVector());
+               //rabund = new RAbundVector(list->getRAbundVector());
                
                if (m->control_pressed) { outputTypes.clear(); delete nameMap; delete read; delete list; delete rabund; return 0; }
                
@@ -234,21 +269,32 @@ int MGClusterCommand::execute(){
                else if (method == "nearest")   { tag = "nn";  }
                else                                                    { tag = "an";  }
                
-               //open output files
-               m->openOutputFile(fileroot+ tag + ".list",  listFile);
-               m->openOutputFile(fileroot+ tag + ".rabund",  rabundFile);
-               m->openOutputFile(fileroot+ tag + ".sabund",  sabundFile);
+        map<string, string> variables; 
+        variables["[filename]"] = fileroot;
+        variables["[clustertag]"] = tag;
+        string sabundFileName = getOutputFileName("sabund", variables);
+        string rabundFileName = getOutputFileName("rabund", variables);
+        if (countfile != "") { variables["[tag2]"] = "unique_list"; }
+        string listFileName = getOutputFileName("list", variables);
+        
+        if (countfile == "") {
+            m->openOutputFile(sabundFileName,  sabundFile);
+            m->openOutputFile(rabundFileName,  rabundFile);
+        }
+               m->openOutputFile(listFileName, listFile);
                
                if (m->control_pressed) { 
                        delete nameMap; delete read; delete list; delete rabund; 
-                       listFile.close(); rabundFile.close(); sabundFile.close(); remove((fileroot+ tag + ".list").c_str()); remove((fileroot+ tag + ".rabund").c_str()); remove((fileroot+ tag + ".sabund").c_str());
+                       listFile.close(); if (countfile == "") { rabundFile.close(); sabundFile.close();  m->mothurRemove((fileroot+ tag + ".rabund")); m->mothurRemove((fileroot+ tag + ".sabund")); } m->mothurRemove((fileroot+ tag + ".list"));
                        outputTypes.clear();
                        return 0; 
                }
                
+               double saveCutoff = cutoff;
+               
                if (!hclusterWanted) {
                        //get distmatrix and overlap
-                       SparseMatrix* distMatrix = read->getDistMatrix();
+                       SparseDistanceMatrix* distMatrix = read->getDistMatrix();
                        overlapMatrix = read->getOverlapMatrix(); //already sorted by read 
                        delete read;
                
@@ -262,19 +308,22 @@ int MGClusterCommand::execute(){
                        
                        if (m->control_pressed) { 
                                delete nameMap; delete distMatrix; delete list; delete rabund; delete cluster;
-                               listFile.close(); rabundFile.close(); sabundFile.close(); remove((fileroot+ tag + ".list").c_str()); remove((fileroot+ tag + ".rabund").c_str()); remove((fileroot+ tag + ".sabund").c_str());
+                               listFile.close(); if (countfile == "") { rabundFile.close(); sabundFile.close();  m->mothurRemove((fileroot+ tag + ".rabund")); m->mothurRemove((fileroot+ tag + ".sabund")); } m->mothurRemove((fileroot+ tag + ".list"));
                                outputTypes.clear();
                                return 0; 
                        }
-               
+            
+            
                        //cluster using cluster classes
                        while (distMatrix->getSmallDist() < cutoff && distMatrix->getNNodes() > 0){
                                
+                if (m->debug) {  cout << "numNodes=" << distMatrix->getNNodes() << " smallDist = " << distMatrix->getSmallDist() << endl; }
+                
                                cluster->update(cutoff);
                                
                                if (m->control_pressed) { 
                                        delete nameMap; delete distMatrix; delete list; delete rabund; delete cluster;
-                                       listFile.close(); rabundFile.close(); sabundFile.close(); remove((fileroot+ tag + ".list").c_str()); remove((fileroot+ tag + ".rabund").c_str()); remove((fileroot+ tag + ".sabund").c_str());
+                                       listFile.close(); if (countfile == "") { rabundFile.close(); sabundFile.close();  m->mothurRemove((fileroot+ tag + ".rabund")); m->mothurRemove((fileroot+ tag + ".sabund")); } m->mothurRemove((fileroot+ tag + ".list"));
                                        outputTypes.clear();
                                        return 0; 
                                }
@@ -297,7 +346,7 @@ int MGClusterCommand::execute(){
                                                
                                                if (m->control_pressed) { 
                                                        delete nameMap; delete distMatrix; delete list; delete rabund; delete cluster; delete temp;
-                                                       listFile.close(); rabundFile.close(); sabundFile.close(); remove((fileroot+ tag + ".list").c_str()); remove((fileroot+ tag + ".rabund").c_str()); remove((fileroot+ tag + ".sabund").c_str());
+                                                       listFile.close(); if (countfile == "") { rabundFile.close(); sabundFile.close();  m->mothurRemove((fileroot+ tag + ".rabund")); m->mothurRemove((fileroot+ tag + ".sabund")); } m->mothurRemove((fileroot+ tag + ".list"));
                                                        outputTypes.clear();
                                                        return 0; 
                                                }
@@ -328,7 +377,7 @@ int MGClusterCommand::execute(){
                                        
                                        if (m->control_pressed) { 
                                                        delete nameMap; delete distMatrix; delete list; delete rabund; delete cluster; delete temp;
-                                                       listFile.close(); rabundFile.close(); sabundFile.close(); remove((fileroot+ tag + ".list").c_str()); remove((fileroot+ tag + ".rabund").c_str()); remove((fileroot+ tag + ".sabund").c_str());
+                                                       listFile.close(); if (countfile == "") { rabundFile.close(); sabundFile.close();  m->mothurRemove((fileroot+ tag + ".rabund")); m->mothurRemove((fileroot+ tag + ".sabund")); } m->mothurRemove((fileroot+ tag + ".list"));
                                                        outputTypes.clear();
                                                        return 0; 
                                        }
@@ -358,7 +407,7 @@ int MGClusterCommand::execute(){
                        
                        if (m->control_pressed) { 
                                delete nameMap;  delete list; delete rabund; 
-                               listFile.close(); rabundFile.close(); sabundFile.close(); remove((fileroot+ tag + ".list").c_str()); remove((fileroot+ tag + ".rabund").c_str()); remove((fileroot+ tag + ".sabund").c_str());
+                               listFile.close(); if (countfile == "") { rabundFile.close(); sabundFile.close();  m->mothurRemove((fileroot+ tag + ".rabund")); m->mothurRemove((fileroot+ tag + ".sabund")); } m->mothurRemove((fileroot+ tag + ".list"));
                                outputTypes.clear();
                                return 0; 
                        }
@@ -375,7 +424,7 @@ int MGClusterCommand::execute(){
                        
                        if (m->control_pressed) { 
                                delete nameMap;  delete list; delete rabund; delete hcluster;
-                               listFile.close(); rabundFile.close(); sabundFile.close(); remove((fileroot+ tag + ".list").c_str()); remove((fileroot+ tag + ".rabund").c_str()); remove((fileroot+ tag + ".sabund").c_str());
+                               listFile.close(); if (countfile == "") { rabundFile.close(); sabundFile.close();  m->mothurRemove((fileroot+ tag + ".rabund")); m->mothurRemove((fileroot+ tag + ".sabund")); } m->mothurRemove((fileroot+ tag + ".list"));
                                outputTypes.clear();
                                return 0; 
                        }
@@ -384,11 +433,16 @@ int MGClusterCommand::execute(){
                
                                seqs = hcluster->getSeqs();
                                
+                               //to account for cutoff change in average neighbor
+                               if (seqs.size() != 0) {
+                                       if (seqs[0].dist > cutoff) { break; }
+                               }
+                               
                                if (m->control_pressed) { 
                                        delete nameMap;  delete list; delete rabund; delete hcluster;
-                                       listFile.close(); rabundFile.close(); sabundFile.close(); remove((fileroot+ tag + ".list").c_str()); remove((fileroot+ tag + ".rabund").c_str()); remove((fileroot+ tag + ".sabund").c_str());
-                                       remove(distFile.c_str());
-                                       remove(overlapFile.c_str());
+                                       listFile.close(); if (countfile == "") { rabundFile.close(); sabundFile.close();  m->mothurRemove((fileroot+ tag + ".rabund")); m->mothurRemove((fileroot+ tag + ".sabund")); } m->mothurRemove((fileroot+ tag + ".list"));
+                                       m->mothurRemove(distFile);
+                                       m->mothurRemove(overlapFile);
                                        outputTypes.clear();
                                        return 0; 
                                }
@@ -397,13 +451,13 @@ int MGClusterCommand::execute(){
                                        
                                        if (seqs[i].seq1 != seqs[i].seq2) {
                
-                                               hcluster->update(seqs[i].seq1, seqs[i].seq2, seqs[i].dist);
+                                               cutoff = hcluster->update(seqs[i].seq1, seqs[i].seq2, seqs[i].dist);
                                                
                                                if (m->control_pressed) { 
                                                        delete nameMap;  delete list; delete rabund; delete hcluster;
-                                                       listFile.close(); rabundFile.close(); sabundFile.close(); remove((fileroot+ tag + ".list").c_str()); remove((fileroot+ tag + ".rabund").c_str()); remove((fileroot+ tag + ".sabund").c_str());
-                                                       remove(distFile.c_str());
-                                                       remove(overlapFile.c_str());
+                                                       listFile.close(); if (countfile == "") { rabundFile.close(); sabundFile.close();  m->mothurRemove((fileroot+ tag + ".rabund")); m->mothurRemove((fileroot+ tag + ".sabund")); } m->mothurRemove((fileroot+ tag + ".list"));
+                                                       m->mothurRemove(distFile);
+                                                       m->mothurRemove(overlapFile);
                                                        outputTypes.clear();
                                                        return 0; 
                                                }
@@ -425,9 +479,9 @@ int MGClusterCommand::execute(){
                                                                
                                                                if (m->control_pressed) { 
                                                                        delete nameMap;  delete list; delete rabund; delete hcluster; delete temp;
-                                                                       listFile.close(); rabundFile.close(); sabundFile.close(); remove((fileroot+ tag + ".list").c_str()); remove((fileroot+ tag + ".rabund").c_str()); remove((fileroot+ tag + ".sabund").c_str());
-                                                                       remove(distFile.c_str());
-                                                                       remove(overlapFile.c_str());
+                                                                       listFile.close(); if (countfile == "") { rabundFile.close(); sabundFile.close();  m->mothurRemove((fileroot+ tag + ".rabund")); m->mothurRemove((fileroot+ tag + ".sabund")); } m->mothurRemove((fileroot+ tag + ".list"));
+                                                                       m->mothurRemove(distFile);
+                                                                       m->mothurRemove(overlapFile);
                                                                        outputTypes.clear();
                                                                        return 0; 
                                                                }
@@ -461,9 +515,9 @@ int MGClusterCommand::execute(){
                                        
                                        if (m->control_pressed) { 
                                                        delete nameMap; delete list; delete rabund; delete hcluster; delete temp;
-                                                       listFile.close(); rabundFile.close(); sabundFile.close(); remove((fileroot+ tag + ".list").c_str()); remove((fileroot+ tag + ".rabund").c_str()); remove((fileroot+ tag + ".sabund").c_str());
-                                                       remove(distFile.c_str());
-                                                       remove(overlapFile.c_str());
+                                                       listFile.close(); if (countfile == "") { rabundFile.close(); sabundFile.close();  m->mothurRemove((fileroot+ tag + ".rabund")); m->mothurRemove((fileroot+ tag + ".sabund")); } m->mothurRemove((fileroot+ tag + ".list"));
+                                                       m->mothurRemove(distFile);
+                                                       m->mothurRemove(overlapFile);
                                                        outputTypes.clear();
                                                        return 0; 
                                        }
@@ -478,35 +532,40 @@ int MGClusterCommand::execute(){
                        }
                        
                        delete hcluster;
-                       remove(distFile.c_str());
-                       remove(overlapFile.c_str());
+                       m->mothurRemove(distFile);
+                       m->mothurRemove(overlapFile);
                }
                
-               delete list; 
+               delete list;
                delete rabund;
                listFile.close();
-               sabundFile.close();
-               rabundFile.close();
-       
-               globaldata->setListFile(fileroot+ tag + ".list");
-               globaldata->setFormat("list");
-               
+        if (countfile == "") {
+            sabundFile.close();
+            rabundFile.close();
+        }
                if (m->control_pressed) { 
                        delete nameMap; 
-                       listFile.close(); rabundFile.close(); sabundFile.close(); remove((fileroot+ tag + ".list").c_str()); remove((fileroot+ tag + ".rabund").c_str()); remove((fileroot+ tag + ".sabund").c_str());
-                       globaldata->setListFile("");
-                       globaldata->setFormat("");
+                       listFile.close(); if (countfile == "") { rabundFile.close(); sabundFile.close();  m->mothurRemove((fileroot+ tag + ".rabund")); m->mothurRemove((fileroot+ tag + ".sabund")); } m->mothurRemove((fileroot+ tag + ".list"));
                        outputTypes.clear();
                        return 0; 
                }
                
                m->mothurOutEndLine();
                m->mothurOut("Output File Names: "); m->mothurOutEndLine();
-               m->mothurOut(fileroot+ tag + ".list"); m->mothurOutEndLine();   outputNames.push_back(fileroot+ tag + ".list"); outputTypes["list"].push_back(fileroot+ tag + ".list");
-               m->mothurOut(fileroot+ tag + ".rabund"); m->mothurOutEndLine(); outputNames.push_back(fileroot+ tag + ".rabund"); outputTypes["rabund"].push_back(fileroot+ tag + ".rabund");
-               m->mothurOut(fileroot+ tag + ".sabund"); m->mothurOutEndLine(); outputNames.push_back(fileroot+ tag + ".sabund"); outputTypes["sabund"].push_back(fileroot+ tag + ".sabund");
+               m->mothurOut(listFileName); m->mothurOutEndLine();      outputNames.push_back(listFileName); outputTypes["list"].push_back(listFileName);
+               if (countfile == "") {
+            m->mothurOut(rabundFileName); m->mothurOutEndLine();       outputNames.push_back(rabundFileName); outputTypes["rabund"].push_back(rabundFileName);
+            m->mothurOut(sabundFileName); m->mothurOutEndLine();       outputNames.push_back(sabundFileName); outputTypes["sabund"].push_back(sabundFileName);
+        }
                m->mothurOutEndLine();
                
+               if (saveCutoff != cutoff) { 
+                       if (hard)       {  saveCutoff = m->ceilDist(saveCutoff, precision);     }
+                       else            {       saveCutoff = m->roundDist(saveCutoff, precision);  }
+                       
+                       m->mothurOut("changed cutoff to " + toString(cutoff)); m->mothurOutEndLine(); 
+               }
+               
                //set list file as new current listfile
                string current = "";
                itTypes = outputTypes.find("list");
@@ -540,12 +599,14 @@ int MGClusterCommand::execute(){
 void MGClusterCommand::printData(ListVector* mergedList){
        try {
                mergedList->print(listFile);
-               mergedList->getRAbundVector().print(rabundFile);
-               
-               SAbundVector sabund = mergedList->getSAbundVector();
+        SAbundVector sabund = mergedList->getSAbundVector();
+        
+        if (countfile == "") {
+            mergedList->getRAbundVector().print(rabundFile);
+            sabund.print(sabundFile);
+        }
 
                sabund.print(cout);
-               sabund.print(sabundFile);
        }
        catch(exception& e) {
                m->errorOut(e, "MGClusterCommand", "printData");
@@ -660,12 +721,12 @@ void MGClusterCommand::sortHclusterFiles(string unsortedDist, string unsortedOve
        try {
                //sort distFile
                string sortedDistFile = m->sortFile(unsortedDist, outputDir);
-               remove(unsortedDist.c_str());  //delete unsorted file
+               m->mothurRemove(unsortedDist);  //delete unsorted file
                distFile = sortedDistFile;
                
                //sort overlap file
                string sortedOverlapFile = m->sortFile(unsortedOverlap, outputDir);
-               remove(unsortedOverlap.c_str());  //delete unsorted file
+               m->mothurRemove(unsortedOverlap);  //delete unsorted file
                overlapFile = sortedOverlapFile;
        }
        catch(exception& e) {
@@ -676,7 +737,33 @@ void MGClusterCommand::sortHclusterFiles(string unsortedDist, string unsortedOve
 
 //**********************************************************************************************************************
 
+void MGClusterCommand::createRabund(CountTable*& ct, ListVector*& list, RAbundVector*& rabund){
+    try {
+        //vector<string> names = ct.getNamesOfSeqs();
 
+        //for ( int i; i < ct.getNumGroups(); i++ ) {    rav.push_back( ct.getNumSeqs(names[i]) );    }
+        //return rav;
+        
+        for(int i = 0; i < list->getNumBins(); i++) { 
+           vector<string> binNames;
+           string bin = list->get(i);
+           m->splitAtComma(bin, binNames);
+           int total = 0;
+           for (int j = 0; j < binNames.size(); j++) { 
+               total += ct->getNumSeqs(binNames[j]);
+           }
+           rabund->push_back(total);   
+       }
+        
+        
+    }
+    catch(exception& e) {
+               m->errorOut(e, "MGClusterCommand", "createRabund");
+               exit(1);
+       }
+    
+}
 
+//**********************************************************************************************************************