]> git.donarmstrong.com Git - mothur.git/blobdiff - indicatorcommand.cpp
indicator command
[mothur.git] / indicatorcommand.cpp
index 966810b1438bd7e9b4072f6a8cccd6cb2d105cee..b06742fe1c529988457300fc7d3305d98e327f0f 100644 (file)
 //**********************************************************************************************************************
 vector<string> IndicatorCommand::setParameters(){      
        try {
-               CommandParameter pdesign("design", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(pdesign);
+               CommandParameter piters("iters", "Number", "", "1000", "", "", "",false,false); parameters.push_back(piters);
+               CommandParameter pdesign("design", "InputTypes", "", "", "TreeDesign", "TreeDesign", "none",false,false); parameters.push_back(pdesign);
                CommandParameter pshared("shared", "InputTypes", "", "", "SharedRel", "SharedRel", "none",false,false); parameters.push_back(pshared);  
                CommandParameter prelabund("relabund", "InputTypes", "", "", "SharedRel", "SharedRel", "none",false,false); parameters.push_back(prelabund);
                CommandParameter pgroups("groups", "String", "", "", "", "", "",false,false); parameters.push_back(pgroups);
                CommandParameter plabel("label", "String", "", "", "", "", "",false,false); parameters.push_back(plabel);
-               CommandParameter ptree("tree", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(ptree);
+               CommandParameter ptree("tree", "InputTypes", "", "", "TreeDesign", "TreeDesign", "none",false,false); parameters.push_back(ptree);
                CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
                CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir);
                
@@ -36,13 +37,14 @@ vector<string> IndicatorCommand::setParameters(){
 string IndicatorCommand::getHelpString(){      
        try {
                string helpString = "";
-               helpString += "The indicator command reads a shared or relabund file and a tree file, and outputs a .indicator.tre and .indicator.summary file. \n";
+               helpString += "The indicator command reads a shared or relabund file and a tree or design file, and outputs a .indicator.tre and .indicator.summary file. \n";
                helpString += "The new tree contains labels at each internal node.  The label is the node number so you can relate the tree to the summary file.\n";
                helpString += "The summary file lists the indicator value for each OTU for each node.\n";
                helpString += "The indicator command parameters are tree, groups, shared, relabund, design and label. The tree parameter is required as well as either shared or relabund.\n";
                helpString += "The design parameter allows you to provide a design file to relate the tree to the shared or relabund file.\n";          
                helpString += "The groups parameter allows you to specify which of the groups in your shared or relabund you would like analyzed, or if you provide a design file the groups in your design file.  The groups may be entered separated by dashes.\n";
                helpString += "The label parameter indicates at what distance your tree relates to the shared or relabund.\n";
+               helpString += "The iters parameter allows you to set number of randomization for the P value.  The default is 1000.";
                helpString += "The indicator command should be used in the following format: indicator(tree=test.tre, shared=test.shared, label=0.03)\n";
                helpString += "Note: No spaces between parameter labels (i.e. tree), '=' and parameters (i.e.yourTreefile).\n"; 
                return helpString;
@@ -142,12 +144,9 @@ IndicatorCommand::IndicatorCommand(string option)  {
 
                        //check for required parameters
                        treefile = validParameter.validFile(parameters, "tree", true);
-                       if (treefile == "not open") { abort = true; }
-                       else if (treefile == "not found") {                             //if there is a current design file, use it
-                               treefile = m->getTreeFile(); 
-                               if (treefile != "") { m->mothurOut("Using " + treefile + " as input file for the tree parameter."); m->mothurOutEndLine(); }
-                               else {  m->mothurOut("You have no current tree file and the tree parameter is required."); m->mothurOutEndLine(); abort = true; }                                                               
-                       }else { m->setTreeFile(treefile); }     
+                       if (treefile == "not open") { treefile = ""; abort = true; }
+                       else if (treefile == "not found") { treefile = "";      }               
+                       else { m->setTreeFile(treefile); }      
                        
                        sharedfile = validParameter.validFile(parameters, "shared", true);
                        if (sharedfile == "not open") { abort = true; }
@@ -160,7 +159,7 @@ IndicatorCommand::IndicatorCommand(string option)  {
                        else { inputFileName = relabundfile; m->setRelAbundFile(relabundfile); }
                        
                        designfile = validParameter.validFile(parameters, "design", true);
-                       if (designfile == "not open") { abort = true; }
+                       if (designfile == "not open") { designfile = ""; abort = true; }
                        else if (designfile == "not found") { designfile = ""; }
                        else { m->setDesignFile(designfile); }
                        
@@ -172,6 +171,9 @@ IndicatorCommand::IndicatorCommand(string option)  {
                        label = validParameter.validFile(parameters, "label", false);                   
                        if (label == "not found") { label = ""; m->mothurOut("You did not provide a label, I will use the first label in your inputfile."); m->mothurOutEndLine(); label=""; }  
                        
+                       string temp = validParameter.validFile(parameters, "iters", false);             if (temp == "not found") { temp = "1000"; }
+                       convert(temp, iters); 
+                       
                        if ((relabundfile == "") && (sharedfile == "")) { 
                                //is there are current file available for either of these?
                                //give priority to shared, then relabund
@@ -187,7 +189,20 @@ IndicatorCommand::IndicatorCommand(string option)  {
                                }
                        }
                        
-                       if ((relabundfile != "") && (sharedfile != "")) { m->mothurOut("You may not use both a shared and relabund file."); m->mothurOutEndLine(); abort = true;  }
+                       
+                       if ((designfile == "") && (treefile == "")) { 
+                               treefile = m->getTreeFile(); 
+                               if (treefile != "") {  m->mothurOut("Using " + treefile + " as input file for the tree parameter."); m->mothurOutEndLine(); }
+                               else { 
+                                       designfile = m->getDesignFile(); 
+                                       if (designfile != "") {  m->mothurOut("Using " + designfile + " as input file for the design parameter."); m->mothurOutEndLine(); }
+                                       else { 
+                                               m->mothurOut("[ERROR]: You must provide either a tree or design file."); m->mothurOutEndLine(); abort = true; 
+                                       }
+                               }
+                       }
+                       
+                       if ((relabundfile != "") && (sharedfile != "")) { m->mothurOut("[ERROR]: You may not use both a shared and relabund file."); m->mothurOutEndLine(); abort = true;  }
                        
                }
        }
@@ -202,6 +217,8 @@ int IndicatorCommand::execute(){
        try {
                
                if (abort == true) { if (calledHelp) { return 0; }  return 2;   }
+               
+               cout.setf(ios::fixed, ios::floatfield); cout.setf(ios::showpoint);
        
                //read designfile if given and set up globaldatas groups for read of sharedfiles
                if (designfile != "") {
@@ -236,101 +253,107 @@ int IndicatorCommand::execute(){
                /***************************************************/
                //    reading tree info                                                    //
                /***************************************************/
-               string groupfile = ""; 
-               m->setTreeFile(treefile);
-               Tree* tree = new Tree(treefile); delete tree;  //extracts names from tree to make faked out groupmap
-               treeMap = new TreeMap();
-               bool mismatch = false;
-                       
-               for (int i = 0; i < m->Treenames.size(); i++) { 
-                       //sanity check - is this a group that is not in the sharedfile?
-                       if (designfile == "") {
-                               if (!(m->inUsersGroups(m->Treenames[i], m->namesOfGroups))) {
-                                       m->mothurOut("[ERROR]: " + m->Treenames[i] + " is not a group in your shared or relabund file."); m->mothurOutEndLine();
-                                       mismatch = true;
-                               }
-                               treeMap->addSeq(m->Treenames[i], "Group1"); 
-                       }else{
-                               vector<string> myGroups; myGroups.push_back(m->Treenames[i]);
-                               vector<string> myNames = designMap->getNamesSeqs(myGroups);
-                               
-                               for(int k = 0; k < myNames.size(); k++) {
-                                       if (!(m->inUsersGroups(myNames[k], m->namesOfGroups))) {
-                                               m->mothurOut("[ERROR]: " + myNames[k] + " is not a group in your shared or relabund file."); m->mothurOutEndLine();
+               if (treefile != "") {
+                       string groupfile = ""; 
+                       m->setTreeFile(treefile);
+                       Tree* tree = new Tree(treefile); delete tree;  //extracts names from tree to make faked out groupmap
+                       treeMap = new TreeMap();
+                       bool mismatch = false;
+                               
+                       for (int i = 0; i < m->Treenames.size(); i++) { 
+                               //sanity check - is this a group that is not in the sharedfile?
+                               if (designfile == "") {
+                                       if (!(m->inUsersGroups(m->Treenames[i], m->namesOfGroups))) {
+                                               m->mothurOut("[ERROR]: " + m->Treenames[i] + " is not a group in your shared or relabund file."); m->mothurOutEndLine();
                                                mismatch = true;
                                        }
+                                       treeMap->addSeq(m->Treenames[i], "Group1"); 
+                               }else{
+                                       vector<string> myGroups; myGroups.push_back(m->Treenames[i]);
+                                       vector<string> myNames = designMap->getNamesSeqs(myGroups);
+                                       
+                                       for(int k = 0; k < myNames.size(); k++) {
+                                               if (!(m->inUsersGroups(myNames[k], m->namesOfGroups))) {
+                                                       m->mothurOut("[ERROR]: " + myNames[k] + " is not a group in your shared or relabund file."); m->mothurOutEndLine();
+                                                       mismatch = true;
+                                               }
+                                       }
+                                       treeMap->addSeq(m->Treenames[i], "Group1");
                                }
-                               treeMap->addSeq(m->Treenames[i], "Group1");
                        }
-               }
-               
-               if ((designfile != "") && (m->Treenames.size() != Groups.size())) { m->mothurOut("[ERROR]: You design file does not match your tree, aborting."); m->mothurOutEndLine(); mismatch = true; }
-                               
-               if (mismatch) { //cleanup and exit
-                       if (designfile != "") { delete designMap; }
-                       if (sharedfile != "") {  for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } }
-                       else { for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } }
-                       delete treeMap;
-                       return 0;
-               }
-        
-               read = new ReadNewickTree(treefile);
-               int readOk = read->read(treeMap); 
-               
-               if (readOk != 0) { m->mothurOut("Read Terminated."); m->mothurOutEndLine(); delete treeMap; delete read; return 0; }
-               
-               vector<Tree*> T = read->getTrees();
-               
-               delete read;
-               
-               if (m->control_pressed) { 
-                       if (designfile != "") { delete designMap; }
-                       if (sharedfile != "") {  for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } }
-                       else { for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } }
-                       for (int i = 0; i < T.size(); i++) {  delete T[i];  }  delete treeMap; return 0; 
-               }
                        
-               T[0]->assembleTree();
+                       if ((designfile != "") && (m->Treenames.size() != Groups.size())) { m->mothurOut("[ERROR]: You design file does not match your tree, aborting."); m->mothurOutEndLine(); mismatch = true; }
+                                       
+                       if (mismatch) { //cleanup and exit
+                               if (designfile != "") { delete designMap; }
+                               if (sharedfile != "") {  for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } }
+                               else { for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } }
+                               delete treeMap;
+                               return 0;
+                       }
+                
+                       read = new ReadNewickTree(treefile);
+                       int readOk = read->read(treeMap); 
+                       
+                       if (readOk != 0) { m->mothurOut("Read Terminated."); m->mothurOutEndLine(); delete treeMap; delete read; return 0; }
+                       
+                       vector<Tree*> T = read->getTrees();
+                       
+                       delete read;
+                       
+                       if (m->control_pressed) { 
+                               if (designfile != "") { delete designMap; }
+                               if (sharedfile != "") {  for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } }
+                               else { for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } }
+                               for (int i = 0; i < T.size(); i++) {  delete T[i];  }  delete treeMap; return 0; 
+                       }
                                
-               /***************************************************/
-               //    create ouptut tree - respecting pickedGroups //
-               /***************************************************/
-               Tree* outputTree = new Tree(m->Groups.size(), treeMap); 
-               
-               outputTree->getSubTree(T[0], m->Groups);
-               outputTree->assembleTree();
+                       T[0]->assembleTree();
+                                       
+                       /***************************************************/
+                       //    create ouptut tree - respecting pickedGroups //
+                       /***************************************************/
+                       Tree* outputTree = new Tree(m->Groups.size(), treeMap); 
                        
-               //no longer need original tree, we have output tree to use and label
-               for (int i = 0; i < T.size(); i++) {  delete T[i];  } 
-               
+                       outputTree->getSubTree(T[0], m->Groups);
+                       outputTree->assembleTree();
                                
-               if (m->control_pressed) { 
-                       if (designfile != "") { delete designMap; }
-                       if (sharedfile != "") {  for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } }
-                       else { for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } }
-                       delete outputTree; delete treeMap;  return 0; 
+                       //no longer need original tree, we have output tree to use and label
+                       for (int i = 0; i < T.size(); i++) {  delete T[i];  } 
+                       
+                                       
+                       if (m->control_pressed) { 
+                               if (designfile != "") { delete designMap; }
+                               if (sharedfile != "") {  for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } }
+                               else { for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } }
+                               delete outputTree; delete treeMap;  return 0; 
+                       }
+                       
+                       /***************************************************/
+                       //              get indicator species values                       //
+                       /***************************************************/
+                       GetIndicatorSpecies(outputTree);
+                       delete outputTree; delete treeMap;
+                       
+               }else { //run with design file only
+                       //get indicator species
+                       GetIndicatorSpecies();
                }
                
-               /***************************************************/
-               //              get indicator species values                       //
-               /***************************************************/
-               GetIndicatorSpecies(outputTree);
-               
                if (designfile != "") { delete designMap; }
                if (sharedfile != "") {  for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } }
                else { for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } }
-               delete outputTree; delete treeMap;
-               
                
                if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) {        remove(outputNames[i].c_str()); } return 0; }
                
                //set tree file as new current treefile
-               string current = "";
-               itTypes = outputTypes.find("tree");
-               if (itTypes != outputTypes.end()) {
-                       if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setTreeFile(current); }
+               if (treefile != "") {
+                       string current = "";
+                       itTypes = outputTypes.find("tree");
+                       if (itTypes != outputTypes.end()) {
+                               if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setTreeFile(current); }
+                       }
                }
-               
                m->mothurOutEndLine();
                m->mothurOut("Output File Names: "); m->mothurOutEndLine();
                for (int i = 0; i < outputNames.size(); i++) {  m->mothurOut(outputNames[i]); m->mothurOutEndLine();    }
@@ -344,6 +367,147 @@ int IndicatorCommand::execute(){
        }
 }
 //**********************************************************************************************************************
+//divide shared or relabund file by groupings in the design file
+//report all otu values to file
+int IndicatorCommand::GetIndicatorSpecies(){
+       try {
+               string thisOutputDir = outputDir;
+               if (outputDir == "") {  thisOutputDir += m->hasPath(inputFileName);  }
+               string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(inputFileName)) + "indicator.summary";
+               outputNames.push_back(outputFileName); outputTypes["summary"].push_back(outputFileName);
+               
+               ofstream out;
+               m->openOutputFile(outputFileName, out);
+               out.setf(ios::fixed, ios::floatfield); out.setf(ios::showpoint);
+               
+               int numBins = 0;
+               if (sharedfile != "") { numBins = lookup[0]->getNumBins(); }
+               else { numBins = lookupFloat[0]->getNumBins(); }
+               
+               if (m->control_pressed) { out.close(); return 0; }
+                       
+               /*****************************************************/
+               //create vectors containing rabund info                          //
+               /*****************************************************/
+                       
+               vector<float> indicatorValues; //size of numBins
+               vector<float> pValues;
+               map< vector<int>, vector<int> > randomGroupingsMap; //maps location in groupings to location in groupings, ie, [0][0] -> [1][2]. This is so we don't have to actually move the sharedRabundVectors.
+                       
+               if (sharedfile != "") {
+                       vector< vector<SharedRAbundVector*> > groupings;
+                       set<string> groupsAlreadyAdded;
+                       vector<SharedRAbundVector*> subset;
+                       
+                       //for each grouping
+                       for (int i = 0; i < designMap->namesOfGroups.size(); i++) {
+                               
+                               for (int k = 0; k < lookup.size(); k++) {
+                                       //are you from this grouping?
+                                       if (designMap->getGroup(lookup[k]->getGroup()) == designMap->namesOfGroups[i]) {
+                                               subset.push_back(lookup[k]);
+                                               groupsAlreadyAdded.insert(lookup[k]->getGroup());
+                                       }
+                               }
+                               if (subset.size() != 0) { groupings.push_back(subset); }
+                               subset.clear();
+                       }
+                               
+                       if (groupsAlreadyAdded.size() != lookup.size()) {  m->mothurOut("[ERROR]: could not make proper groupings."); m->mothurOutEndLine(); }
+                               
+                       indicatorValues = getValues(groupings, randomGroupingsMap);
+                       
+                       pValues.resize(indicatorValues.size(), 0);
+                       for(int i=0;i<iters;i++){
+                               if (m->control_pressed) { break; }
+                               randomGroupingsMap = randomizeGroupings(groupings, lookup.size());
+                               vector<float> randomIndicatorValues = getValues(groupings, randomGroupingsMap);
+                               
+                               for (int j = 0; j < indicatorValues.size(); j++) {
+                                       if (randomIndicatorValues[j] >= indicatorValues[j]) { pValues[j]++; }
+                               }
+                       }
+                       
+                       for (int i = 0; i < pValues.size(); i++) { pValues[i] /= (double)iters; }
+                               
+               }else {
+                       vector< vector<SharedRAbundFloatVector*> > groupings;
+                       set<string> groupsAlreadyAdded;
+                       vector<SharedRAbundFloatVector*> subset;
+                       
+                       //for each grouping
+                       for (int i = 0; i < designMap->namesOfGroups.size(); i++) {
+                               for (int k = 0; k < lookupFloat.size(); k++) {
+                                       //are you from this grouping?
+                                       if (designMap->getGroup(lookupFloat[k]->getGroup()) == designMap->namesOfGroups[i]) {
+                                               subset.push_back(lookupFloat[k]);
+                                               groupsAlreadyAdded.insert(lookupFloat[k]->getGroup());
+                                       }
+                               }
+                               if (subset.size() != 0) { groupings.push_back(subset); }
+                               subset.clear();
+                       }
+                       
+                       if (groupsAlreadyAdded.size() != lookupFloat.size()) {  m->mothurOut("[ERROR]: could not make proper groupings."); m->mothurOutEndLine(); }
+                       
+                       indicatorValues = getValues(groupings, randomGroupingsMap);
+                       
+                       pValues.resize(indicatorValues.size(), 0);
+                       for(int i=0;i<iters;i++){
+                               if (m->control_pressed) { break; }
+                               randomGroupingsMap = randomizeGroupings(groupings, lookupFloat.size());
+                               vector<float> randomIndicatorValues = getValues(groupings, randomGroupingsMap);
+                               
+                               for (int j = 0; j < indicatorValues.size(); j++) {
+                                       if (randomIndicatorValues[j] >= indicatorValues[j]) { pValues[j]++; }
+                               }
+                       }
+                       
+                       for (int i = 0; i < pValues.size(); i++) { pValues[i] /= (double)iters; }
+               }
+                       
+               if (m->control_pressed) { out.close(); return 0; }
+                       
+                       
+               /******************************************************/
+               //output indicator values to table form               //
+               /*****************************************************/
+               int indicatorOTU;
+               double maxValue, pvalue; maxValue=0.0; pvalue=0.0;
+               out << "OTU\tIndicator_Value\tpValue" << endl;
+               for (int j = 0; j < indicatorValues.size(); j++) {
+                               
+                       if (m->control_pressed) { out.close(); return 0; }
+                       
+                       out << (j+1) << '\t' << indicatorValues[j] << '\t'; 
+                       
+                       if (pValues[j] > (1/(float)iters)) { out << pValues[j] << endl; } 
+                       else { out << "<" << (1/(float)iters) << endl; }
+                       
+                       if (maxValue < indicatorValues[j]) { 
+                               maxValue = indicatorValues[j];
+                               indicatorOTU = j;
+                       }
+               }
+               
+               m->mothurOutEndLine(); m->mothurOut("Species\tIndicatorValue\tpValue\n");
+               cout << "OTU" << indicatorOTU+1 << '\t' << maxValue << '\t';
+               string pValueString = "<" + toString((1/(float)iters)); 
+               if (pValues[indicatorOTU] > (1/(float)iters)) { pValueString = toString(pValues[indicatorOTU]); cout << pValues[indicatorOTU];} 
+               else { cout << "<" << (1/(float)iters); }
+               m->mothurOutJustToLog("OTU" + toString(indicatorOTU+1) + "\t" + toString(maxValue) + "\t" + pValueString); 
+               m->mothurOutEndLine(); 
+               
+               out.close();
+               
+               return 0;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "IndicatorCommand", "GetIndicatorSpecies");      
+               exit(1);
+       }
+}
+//**********************************************************************************************************************
 //traverse tree finding indicator species values for each otu at each node
 //label node with otu number that has highest indicator value
 //report all otu values to file
@@ -365,9 +529,11 @@ int IndicatorCommand::GetIndicatorSpecies(Tree*& T){
                
                //print headings
                out << "TreeNode\t";
-               for (int i = 0; i < numBins; i++) { out << "OTU-" << (i+1) << '\t'; }
+               for (int i = 0; i < numBins; i++) { out << "OTU" << (i+1) << "_IndValue" << '\t' << "pValue" << '\t'; }
                out << endl;
                
+               m->mothurOutEndLine(); m->mothurOut("Node\tSpecies\tIndicatorValue\tpValue\n");
+               
                string treeOutputDir = outputDir;
                if (outputDir == "") {  treeOutputDir += m->hasPath(treefile);  }
                string outputTreeFileName = treeOutputDir + m->getRootName(m->getSimpleName(treefile)) + "indicator.tre";
@@ -396,6 +562,8 @@ int IndicatorCommand::GetIndicatorSpecies(Tree*& T){
                        /*****************************************************/
                        
                        vector<float> indicatorValues; //size of numBins
+                       vector<float> pValues;
+                       map< vector<int>, vector<int> > randomGroupingsMap; //maps location in groupings to location in groupings, ie, [0][0] -> [1][2]. This is so we don't have to actually move the sharedRabundVectors.
                        
                        if (sharedfile != "") {
                                vector< vector<SharedRAbundVector*> > groupings;
@@ -446,7 +614,20 @@ int IndicatorCommand::GetIndicatorSpecies(Tree*& T){
                                
                                if (groupsAlreadyAdded.size() != lookup.size()) {  m->mothurOut("[ERROR]: could not make proper groupings."); m->mothurOutEndLine(); }
                                                                
-                               indicatorValues = getValues(groupings);
+                               indicatorValues = getValues(groupings, randomGroupingsMap);
+                               
+                               pValues.resize(indicatorValues.size(), 0);
+                               for(int i=0;i<iters;i++){
+                                       if (m->control_pressed) { break; }
+                                       randomGroupingsMap = randomizeGroupings(groupings, lookup.size());
+                                       vector<float> randomIndicatorValues = getValues(groupings, randomGroupingsMap);
+                                       
+                                       for (int j = 0; j < indicatorValues.size(); j++) {
+                                               if (randomIndicatorValues[j] >= indicatorValues[j]) { pValues[j]++; }
+                                       }
+                               }
+                               
+                               for (int i = 0; i < pValues.size(); i++) { pValues[i] /= (double)iters; }
                                
                        }else {
                                vector< vector<SharedRAbundFloatVector*> > groupings;
@@ -494,7 +675,21 @@ int IndicatorCommand::GetIndicatorSpecies(Tree*& T){
                                
                                if (groupsAlreadyAdded.size() != lookupFloat.size()) { m->mothurOut("[ERROR]: could not make proper groupings."); m->mothurOutEndLine(); }
                                
-                               indicatorValues = getValues(groupings);
+                               indicatorValues = getValues(groupings, randomGroupingsMap);
+                               
+                               pValues.resize(indicatorValues.size(), 0);
+                               for(int i=0;i<iters;i++){
+                                       
+                                       if (m->control_pressed) { break; }
+                                       randomGroupingsMap = randomizeGroupings(groupings, lookup.size());
+                                       vector<float> randomIndicatorValues = getValues(groupings, randomGroupingsMap);
+                                       
+                                       for (int j = 0; j < indicatorValues.size(); j++) {
+                                               if (randomIndicatorValues[j] >= indicatorValues[j]) { pValues[j]++; }
+                                       }
+                               }
+                               
+                               for (int i = 0; i < pValues.size(); i++) { pValues[i] /= (double)iters; }
                        }
                        
                        if (m->control_pressed) { out.close(); return 0; }
@@ -504,16 +699,35 @@ int IndicatorCommand::GetIndicatorSpecies(Tree*& T){
                        //output indicator values to table form + label tree  //
                        /*****************************************************/
                        out << (i+1) << '\t';
+                       int indicatorOTU;
+                       double maxValue, pvalue; maxValue=0.0; pvalue=0.0;
                        for (int j = 0; j < indicatorValues.size(); j++) {
                                
                                if (m->control_pressed) { out.close(); return 0; }
                                
-                               out << indicatorValues[j] << '\t';
+                               if (pValues[j] < (1/(float)iters)) {
+                                       out << indicatorValues[j] << '\t' << '<' << (1/(float)iters) << '\t';
+                               }else {
+                                       out << indicatorValues[j] << '\t' << pValues[j] << '\t';
+                               }
+                               
+                               if (maxValue < indicatorValues[j]) { 
+                                       maxValue = indicatorValues[j];
+                                       indicatorOTU = j;
+                               }
                        }
                        out << endl;
                        
                        T->tree[i].setLabel((i+1));
                        
+                       cout << i+1 << "\tOTU" << indicatorOTU+1 << '\t' << maxValue << '\t';
+                       string pValueString = "<" + toString((1/(float)iters)); 
+                       if (pValues[indicatorOTU] > (1/(float)iters)) { pValueString = toString(pValues[indicatorOTU]); cout << pValues[indicatorOTU];} 
+                       else { cout << "<" << (1/(float)iters); }
+                       m->mothurOutJustToLog(toString(i) + "\tOTU" + toString(indicatorOTU+1) + "\t" + toString(maxValue) + "\t" + pValueString); 
+                       m->mothurOutEndLine(); 
+                       
+                       
                }
                out.close();
        
@@ -532,9 +746,10 @@ int IndicatorCommand::GetIndicatorSpecies(Tree*& T){
        }
 }
 //**********************************************************************************************************************
-vector<float> IndicatorCommand::getValues(vector< vector<SharedRAbundFloatVector*> >& groupings){
+vector<float> IndicatorCommand::getValues(vector< vector<SharedRAbundFloatVector*> >& groupings, map< vector<int>, vector<int> > groupingsMap){
        try {
                vector<float> values;
+               map< vector<int>, vector<int> >::iterator it;
                
                //for each otu
                for (int i = 0; i < groupings[0][0]->getNumBins(); i++) {
@@ -551,8 +766,17 @@ vector<float> IndicatorCommand::getValues(vector< vector<SharedRAbundFloatVector
                                float totalAbund = 0;
                                int numNotZero = 0;
                                for (int k = 0; k < groupings[j].size(); k++) { 
-                                       totalAbund += groupings[j][k]->getAbundance(i); 
-                                       if (groupings[j][k]->getAbundance(i) != 0) { numNotZero++; }
+                                       vector<int> temp; temp.push_back(j); temp.push_back(k);
+                                       it = groupingsMap.find(temp);
+                                       
+                                       if (it == groupingsMap.end()) { //this one didnt get moved
+                                               totalAbund += groupings[j][k]->getAbundance(i); 
+                                               if (groupings[j][k]->getAbundance(i) != 0.0) { numNotZero++; }
+                                       }else {
+                                               totalAbund += groupings[(it->second)[0]][(it->second)[1]]->getAbundance(i); 
+                                               if (groupings[(it->second)[0]][(it->second)[1]]->getAbundance(i) != 0.0) { numNotZero++; }
+                                       }
+                                       
                                }
                                
                                //mean abundance
@@ -586,7 +810,7 @@ vector<float> IndicatorCommand::getValues(vector< vector<SharedRAbundFloatVector
 }
 //**********************************************************************************************************************
 //same as above, just data type difference
-vector<float> IndicatorCommand::getValues(vector< vector<SharedRAbundVector*> >& groupings){
+vector<float> IndicatorCommand::getValues(vector< vector<SharedRAbundVector*> >& groupings, map< vector<int>, vector<int> > groupingsMap){
        try {
                vector<float> values;
                
@@ -596,6 +820,8 @@ vector<float> IndicatorCommand::getValues(vector< vector<SharedRAbundVector*> >&
                        cout << groupings[j][k]->getGroup() << endl;
                }
        }*/
+               map< vector<int>, vector<int> >::iterator it;
+               
                //for each otu
                for (int i = 0; i < groupings[0][0]->getNumBins(); i++) {
                        vector<float> terms; 
@@ -607,8 +833,16 @@ vector<float> IndicatorCommand::getValues(vector< vector<SharedRAbundVector*> >&
                                int totalAbund = 0.0;
                                int numNotZero = 0;
                                for (int k = 0; k < groupings[j].size(); k++) { 
-                                       totalAbund += groupings[j][k]->getAbundance(i); 
-                                       if (groupings[j][k]->getAbundance(i) != 0.0) { numNotZero++; }
+                                       vector<int> temp; temp.push_back(j); temp.push_back(k);
+                                       it = groupingsMap.find(temp);
+                                       
+                                       if (it == groupingsMap.end()) { //this one didnt get moved
+                                               totalAbund += groupings[j][k]->getAbundance(i); 
+                                               if (groupings[j][k]->getAbundance(i) != 0.0) { numNotZero++; }
+                                       }else {
+                                               totalAbund += groupings[(it->second)[0]][(it->second)[1]]->getAbundance(i); 
+                                               if (groupings[(it->second)[0]][(it->second)[1]]->getAbundance(i) != 0.0) { numNotZero++; }
+                                       }
                                        
                                }
                                
@@ -901,7 +1135,76 @@ int IndicatorCommand::getSharedFloat(){
                m->errorOut(e, "IndicatorCommand", "getShared");        
        exit(1);
        }
-}              
+}      
+//**********************************************************************************************************************
+//swap groups between groupings, in essence randomizing the second column of the design file
+map< vector<int>, vector<int> > IndicatorCommand::randomizeGroupings(vector< vector<SharedRAbundVector*> >& groupings, int numLookupGroups){
+       try {
+               
+               map< vector<int>, vector<int> > randomGroupings; 
+               
+               for (int i = 0; i < numLookupGroups; i++) {
+                       if (m->control_pressed) {break;}
+                       
+                       //get random groups to swap to switch with
+                       //generate random int between 0 and groupings.size()-1
+                       int z = m->getRandomIndex(groupings.size()-1);
+                       int x = m->getRandomIndex(groupings.size()-1);
+                       int a = m->getRandomIndex(groupings[z].size()-1);
+                       int b = m->getRandomIndex(groupings[x].size()-1);
+                       //cout << i << '\t' << z << '\t' << x << '\t' << a << '\t' << b << endl;        
+                       //if ((z < 0) || (z > 1) || x<0 || x>1 || a <0 || a>groupings[z].size()-1 || b<0 || b>groupings[x].size()-1) { cout << "probelm" << i << '\t' << z << '\t' << x << '\t' << a << '\t' << b << endl;      }
+                       
+                       vector<int> from;
+                       vector<int> to;
+                       
+                       from.push_back(z); from.push_back(a);
+                       to.push_back(x); to.push_back(b);
+                       
+                       randomGroupings[from] = to;
+               }
+               //cout << "done" << endl;       
+               return randomGroupings;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "IndicatorCommand", "randomizeGroupings");       
+               exit(1);
+       }
+}      
+//**********************************************************************************************************************
+//swap groups between groupings, in essence randomizing the second column of the design file
+map< vector<int>, vector<int> > IndicatorCommand::randomizeGroupings(vector< vector<SharedRAbundFloatVector*> >& groupings, int numLookupGroups){
+       try {
+               
+               map< vector<int>, vector<int> > randomGroupings; 
+               
+               for (int i = 0; i < numLookupGroups; i++) {
+                       
+                       //get random groups to swap to switch with
+                       //generate random int between 0 and groupings.size()-1
+                       int z = m->getRandomIndex(groupings.size()-1);
+                       int x = m->getRandomIndex(groupings.size()-1);
+                       int a = m->getRandomIndex(groupings[z].size()-1);
+                       int b = m->getRandomIndex(groupings[x].size()-1);
+                       //cout << i << '\t' << z << '\t' << x << '\t' << a << '\t' << b << endl;                
+                       
+                       vector<int> from;
+                       vector<int> to;
+                       
+                       from.push_back(z); from.push_back(a);
+                       to.push_back(x); to.push_back(b);
+                       
+                       randomGroupings[from] = to;
+               }
+               
+               return randomGroupings;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "IndicatorCommand", "randomizeGroupings");       
+               exit(1);
+       }
+}                      
+                                                               
 /*****************************************************************/