]> git.donarmstrong.com Git - mothur.git/blobdiff - indicatorcommand.cpp
added getCommandInfoCommand for gui
[mothur.git] / indicatorcommand.cpp
index 7f6dc21d21b1d3b0ebf3e68e91011e53549bd46d..01bc2f1d7405093db6a3f5fc4b44a0c17e76fbd3 100644 (file)
@@ -8,35 +8,56 @@
  */
 
 #include "indicatorcommand.h"
+#include "sharedutilities.h"
+
+
 //**********************************************************************************************************************
-vector<string> IndicatorCommand::getValidParameters(){ 
+vector<string> IndicatorCommand::setParameters(){      
        try {
-               string Array[] =  {"tree","shared","relabund","label","groups","outputdir","inputdir"};
-               vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
+               CommandParameter pdesign("design", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(pdesign);
+               CommandParameter pshared("shared", "InputTypes", "", "", "SharedRel", "SharedRel", "none",false,false); parameters.push_back(pshared);  
+               CommandParameter prelabund("relabund", "InputTypes", "", "", "SharedRel", "SharedRel", "none",false,false); parameters.push_back(prelabund);
+               CommandParameter pgroups("groups", "String", "", "", "", "", "",false,false); parameters.push_back(pgroups);
+               CommandParameter plabel("label", "String", "", "", "", "", "",false,false); parameters.push_back(plabel);
+               CommandParameter ptree("tree", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(ptree);
+               CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
+               CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir);
+               
+               vector<string> myArray;
+               for (int i = 0; i < parameters.size(); i++) {   myArray.push_back(parameters[i].name);          }
                return myArray;
        }
        catch(exception& e) {
-               m->errorOut(e, "IndicatorCommand", "getValidParameters");
+               m->errorOut(e, "IndicatorCommand", "setParameters");
                exit(1);
        }
 }
 //**********************************************************************************************************************
-vector<string> IndicatorCommand::getRequiredParameters(){      
+string IndicatorCommand::getHelpString(){      
        try {
-               string Array[] =  {"label","tree"};
-               vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
-               return myArray;
+               string helpString = "";
+               helpString += "The indicator command reads a shared or relabund file and a tree file, and outputs a .indicator.tre and .indicator.summary file. \n";
+               helpString += "The new tree contains labels at each internal node.  The label is the node number so you can relate the tree to the summary file.\n";
+               helpString += "The summary file lists the indicator value for each OTU for each node.\n";
+               helpString += "The indicator command parameters are tree, groups, shared, relabund, design and label. The tree parameter is required as well as either shared or relabund.\n";
+               helpString += "The design parameter allows you to provide a design file to relate the tree to the shared or relabund file.\n";          
+               helpString += "The groups parameter allows you to specify which of the groups in your shared or relabund you would like analyzed, or if you provide a design file the groups in your design file.  The groups may be entered separated by dashes.\n";
+               helpString += "The label parameter indicates at what distance your tree relates to the shared or relabund.\n";
+               helpString += "The indicator command should be used in the following format: indicator(tree=test.tre, shared=test.shared, label=0.03)\n";
+               helpString += "Note: No spaces between parameter labels (i.e. tree), '=' and parameters (i.e.yourTreefile).\n"; 
+               return helpString;
        }
        catch(exception& e) {
-               m->errorOut(e, "IndicatorCommand", "getRequiredParameters");
+               m->errorOut(e, "IndicatorCommand", "getHelpString");
                exit(1);
        }
 }
+
 //**********************************************************************************************************************
 IndicatorCommand::IndicatorCommand(){  
        try {
-               abort = true;
-               //initialize outputTypes
+               abort = true; calledHelp = true; 
+               setParameters();
                vector<string> tempOutNames;
                outputTypes["tree"] = tempOutNames;
                outputTypes["summary"] = tempOutNames;
@@ -46,31 +67,16 @@ IndicatorCommand::IndicatorCommand(){
                exit(1);
        }
 }
-
-//**********************************************************************************************************************
-vector<string> IndicatorCommand::getRequiredFiles(){   
-       try {
-               vector<string> myArray;
-               return myArray;
-       }
-       catch(exception& e) {
-               m->errorOut(e, "IndicatorCommand", "getRequiredFiles");
-               exit(1);
-       }
-}
 //**********************************************************************************************************************
 IndicatorCommand::IndicatorCommand(string option)  {
        try {
-               globaldata = GlobalData::getInstance();
-               abort = false;
+               abort = false; calledHelp = false;   
                
                //allow user to run help
-               if(option == "help") { help(); abort = true; }
+               if(option == "help") { help(); abort = true; calledHelp = true; }
                
                else {
-                       //valid paramters for this command
-                       string Array[] =  {"tree","shared","relabund","groups","label","outputdir","inputdir"};
-                       vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
+                       vector<string> myArray = setParameters();
                        
                        OptionParser parser(option);
                        map<string, string> parameters = parser.getParameters();
@@ -83,7 +89,7 @@ IndicatorCommand::IndicatorCommand(string option)  {
                                if (validParameter.isValidParameter(it->first, myArray, it->second) != true) {  abort = true;  }
                        }
                        
-                       globaldata->newRead();
+                       m->runParse = true;
                        
                        vector<string> tempOutNames;
                        outputTypes["tree"] = tempOutNames;
@@ -118,6 +124,13 @@ IndicatorCommand::IndicatorCommand(string option)  {
                                        if (path == "") {       parameters["relabund"] = inputDir + it->second;         }
                                }
                                
+                               it = parameters.find("design");
+                               //user has given a template file
+                               if(it != parameters.end()){ 
+                                       path = m->hasPath(it->second);
+                                       //if the user has not given a path then, add inputdir. else leave path alone.
+                                       if (path == "") {       parameters["design"] = inputDir + it->second;           }
+                               }
                        }
                        
                        outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){  outputDir = ""; }
@@ -125,8 +138,11 @@ IndicatorCommand::IndicatorCommand(string option)  {
                        //check for required parameters
                        treefile = validParameter.validFile(parameters, "tree", true);
                        if (treefile == "not open") { abort = true; }
-                       else if (treefile == "not found") { treefile = ""; m->mothurOut("tree is a required parameter for the indicator command."); m->mothurOutEndLine(); abort = true;  }     
-                       else {  globaldata->setTreeFile(treefile);  globaldata->setFormat("tree");      }
+                       else if (treefile == "not found") {                             //if there is a current design file, use it
+                               treefile = m->getTreeFile(); 
+                               if (treefile != "") { m->mothurOut("Using " + treefile + " as input file for the tree parameter."); m->mothurOutEndLine(); }
+                               else {  m->mothurOut("You have no current tree file and the tree parameter is required."); m->mothurOutEndLine(); abort = true; }                                                               
+                       }       
                        
                        sharedfile = validParameter.validFile(parameters, "shared", true);
                        if (sharedfile == "not open") { abort = true; }
@@ -138,18 +154,32 @@ IndicatorCommand::IndicatorCommand(string option)  {
                        else if (relabundfile == "not found") { relabundfile = ""; }
                        else { inputFileName = relabundfile; }
                        
+                       designfile = validParameter.validFile(parameters, "design", true);
+                       if (designfile == "not open") { abort = true; }
+                       else if (designfile == "not found") { designfile = ""; }
+                       
                        groups = validParameter.validFile(parameters, "groups", false);                 
-                       if (groups == "not found") { groups = ""; pickedGroups = false; }
-                       else { 
-                               pickedGroups = true;
-                               m->splitAtDash(groups, Groups);
-                               globaldata->Groups = Groups;
-                       }                       
+                       if (groups == "not found") { groups = "";  Groups.push_back("all"); }
+                       else { m->splitAtDash(groups, Groups);  }                       
+                       m->Groups = Groups;
                        
                        label = validParameter.validFile(parameters, "label", false);                   
-                       if (label == "not found") { label = ""; m->mothurOut("You must provide a label to process."); m->mothurOutEndLine(); abort = true; }    
-                       
-                       if ((relabundfile == "") && (sharedfile == "")) { m->mothurOut("You must provide either a shared or relabund file."); m->mothurOutEndLine(); abort = true;  }
+                       if (label == "not found") { label = ""; m->mothurOut("You did not provide a label, I will use the first label in your inputfile."); m->mothurOutEndLine(); label=""; }  
+                       
+                       if ((relabundfile == "") && (sharedfile == "")) { 
+                               //is there are current file available for either of these?
+                               //give priority to shared, then relabund
+                               sharedfile = m->getSharedFile(); 
+                               if (sharedfile != "") {  inputFileName = sharedfile; m->mothurOut("Using " + sharedfile + " as input file for the shared parameter."); m->mothurOutEndLine(); }
+                               else { 
+                                       relabundfile = m->getRelAbundFile(); 
+                                       if (relabundfile != "") {  inputFileName = relabundfile; m->mothurOut("Using " + relabundfile + " as input file for the relabund parameter."); m->mothurOutEndLine(); }
+                                       else { 
+                                               m->mothurOut("No valid current files. You must provide a shared or relabund."); m->mothurOutEndLine(); 
+                                               abort = true;
+                                       }
+                               }
+                       }
                        
                        if ((relabundfile != "") && (sharedfile != "")) { m->mothurOut("You may not use both a shared and relabund file."); m->mothurOutEndLine(); abort = true;  }
                        
@@ -162,86 +192,95 @@ IndicatorCommand::IndicatorCommand(string option)  {
 }
 //**********************************************************************************************************************
 
-void IndicatorCommand::help(){
-       try {
-               /*m->mothurOut("The read.tree command must be run before you execute a unifrac.weighted, unifrac.unweighted. \n");
-               m->mothurOut("It also must be run before using the parsimony command, unless you are using the randomtree parameter.\n");
-               m->mothurOut("The read.tree command parameters are tree, group and name.\n");
-               m->mothurOut("The read.tree command should be in the following format: read.tree(tree=yourTreeFile, group=yourGroupFile).\n");
-               m->mothurOut("The tree and group parameters are both required, if no group file is given then one group is assumed.\n");
-               m->mothurOut("The name parameter allows you to enter a namefile.\n");
-               m->mothurOut("Note: No spaces between parameter labels (i.e. tree), '=' and parameters (i.e.yourTreefile).\n\n"); */
-       }
-       catch(exception& e) {
-               m->errorOut(e, "IndicatorCommand", "help");     
-               exit(1);
-       }
-}
-
-//**********************************************************************************************************************
-
-IndicatorCommand::~IndicatorCommand(){}
-
-//**********************************************************************************************************************
-
 int IndicatorCommand::execute(){
        try {
                
-               if (abort == true) { return 0; }
+               if (abort == true) { if (calledHelp) { return 0; }  return 2;   }
+       
+               //read designfile if given and set up globaldatas groups for read of sharedfiles
+               if (designfile != "") {
+                       designMap = new GroupMap(designfile);
+                       designMap->readDesignMap();
+                       
+                       //fill Groups - checks for "all" and for any typo groups
+                       SharedUtil* util = new SharedUtil();
+                       util->setGroups(Groups, designMap->namesOfGroups);
+                       delete util;
+                       
+                       //loop through the Groups and fill Globaldata's Groups with the design file info
+                       m->Groups = designMap->getNamesSeqs(Groups);
+               }
        
                /***************************************************/
                // use smart distancing to get right sharedRabund  //
                /***************************************************/
                if (sharedfile != "") {  
                        getShared(); 
-                       if (m->control_pressed) {  for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } return 0; }
+                       if (m->control_pressed) { if (designfile != "") { delete designMap; } for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } return 0; }
                        if (lookup[0] == NULL) { m->mothurOut("[ERROR] reading shared file."); m->mothurOutEndLine(); return 0; }
                }else { 
                        getSharedFloat(); 
-                       if (m->control_pressed) {  for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } return 0; }
+                       if (m->control_pressed) {  if (designfile != "") { delete designMap; } for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } return 0; }
                        if (lookupFloat[0] == NULL) { m->mothurOut("[ERROR] reading relabund file."); m->mothurOutEndLine(); return 0; }
                }
+               
+               //reset Globaldatas groups if needed
+               if (designfile != "") { m->Groups = Groups; }
                        
                /***************************************************/
                //    reading tree info                                                    //
                /***************************************************/
                string groupfile = ""; 
                Tree* tree = new Tree(treefile); delete tree;  //extracts names from tree to make faked out groupmap
-        
-               globaldata->setGroupFile(groupfile); 
                treeMap = new TreeMap();
                bool mismatch = false;
-               for (int i = 0; i < globaldata->Treenames.size(); i++) { 
+                       
+               for (int i = 0; i < m->Treenames.size(); i++) { 
                        //sanity check - is this a group that is not in the sharedfile?
-                       if (!(m->inUsersGroups(globaldata->Treenames[i], globaldata->gGroupmap->namesOfGroups))) {
-                               m->mothurOut("[ERROR]: " + globaldata->Treenames[i] + " is not a group in your shared or relabund file."); m->mothurOutEndLine();
-                               mismatch = true;
+                       if (designfile == "") {
+                               if (!(m->inUsersGroups(m->Treenames[i], m->namesOfGroups))) {
+                                       m->mothurOut("[ERROR]: " + m->Treenames[i] + " is not a group in your shared or relabund file."); m->mothurOutEndLine();
+                                       mismatch = true;
+                               }
+                               treeMap->addSeq(m->Treenames[i], "Group1"); 
+                       }else{
+                               vector<string> myGroups; myGroups.push_back(m->Treenames[i]);
+                               vector<string> myNames = designMap->getNamesSeqs(myGroups);
+                               
+                               for(int k = 0; k < myNames.size(); k++) {
+                                       if (!(m->inUsersGroups(myNames[k], m->namesOfGroups))) {
+                                               m->mothurOut("[ERROR]: " + myNames[k] + " is not a group in your shared or relabund file."); m->mothurOutEndLine();
+                                               mismatch = true;
+                                       }
+                               }
+                               treeMap->addSeq(m->Treenames[i], "Group1");
                        }
-                       treeMap->addSeq(globaldata->Treenames[i], "Group1"); 
                }
+               
+               if ((designfile != "") && (m->Treenames.size() != Groups.size())) { m->mothurOut("[ERROR]: You design file does not match your tree, aborting."); m->mothurOutEndLine(); mismatch = true; }
                                
                if (mismatch) { //cleanup and exit
+                       if (designfile != "") { delete designMap; }
                        if (sharedfile != "") {  for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } }
                        else { for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } }
                        delete treeMap;
                        return 0;
                }
-                       
-               globaldata->gTreemap = treeMap;
         
                read = new ReadNewickTree(treefile);
-               int readOk = read->read(); 
+               int readOk = read->read(treeMap); 
                
-               if (readOk != 0) { m->mothurOut("Read Terminated."); m->mothurOutEndLine(); globaldata->gTree.clear(); delete globaldata->gTreemap; delete read; return 0; }
+               if (readOk != 0) { m->mothurOut("Read Terminated."); m->mothurOutEndLine(); delete treeMap; delete read; return 0; }
                
-               vector<Tree*> T = globaldata->gTree;
+               vector<Tree*> T = read->getTrees();
                
                delete read;
                
-               if (m->control_pressed) {  
+               if (m->control_pressed) { 
+                       if (designfile != "") { delete designMap; }
                        if (sharedfile != "") {  for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } }
                        else { for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } }
-                       for (int i = 0; i < T.size(); i++) {  delete T[i];  } globaldata->gTree.clear(); delete globaldata->gTreemap; return 0; 
+                       for (int i = 0; i < T.size(); i++) {  delete T[i];  }  delete treeMap; return 0; 
                }
                        
                T[0]->assembleTree();
@@ -249,23 +288,20 @@ int IndicatorCommand::execute(){
                /***************************************************/
                //    create ouptut tree - respecting pickedGroups //
                /***************************************************/
-               Tree* outputTree = new Tree(globaldata->Groups.size()); 
-               
-               if (pickedGroups) {
-                       outputTree->getSubTree(T[0], globaldata->Groups);
-                       outputTree->assembleTree();
-               }else{
-                       outputTree->getCopy(T[0]);
-                       outputTree->assembleTree();
-               }
+               Tree* outputTree = new Tree(m->Groups.size(), treeMap); 
                
+               outputTree->getSubTree(T[0], m->Groups);
+               outputTree->assembleTree();
+                       
                //no longer need original tree, we have output tree to use and label
-               for (int i = 0; i < T.size(); i++) {  delete T[i];  } globaldata->gTree.clear();
+               for (int i = 0; i < T.size(); i++) {  delete T[i];  } 
                
-               if (m->control_pressed) {  
+                               
+               if (m->control_pressed) { 
+                       if (designfile != "") { delete designMap; }
                        if (sharedfile != "") {  for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } }
                        else { for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } }
-                       delete outputTree; delete globaldata->gTreemap;  return 0; 
+                       delete outputTree; delete treeMap;  return 0; 
                }
                
                /***************************************************/
@@ -273,11 +309,19 @@ int IndicatorCommand::execute(){
                /***************************************************/
                GetIndicatorSpecies(outputTree);
                
-               if (m->control_pressed) {  
-                       if (sharedfile != "") {  for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } }
-                       else { for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } }
-                       for (int i = 0; i < outputNames.size(); i++) {  remove(outputNames[i].c_str()); }
-                       delete outputTree; delete globaldata->gTreemap;  return 0; 
+               if (designfile != "") { delete designMap; }
+               if (sharedfile != "") {  for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  } }
+               else { for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  } }
+               delete outputTree; delete treeMap;
+               
+               
+               if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) {        remove(outputNames[i].c_str()); } return 0; }
+               
+               //set tree file as new current treefile
+               string current = "";
+               itTypes = outputTypes.find("tree");
+               if (itTypes != outputTypes.end()) {
+                       if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setTreeFile(current); }
                }
                
                m->mothurOutEndLine();
@@ -298,6 +342,7 @@ int IndicatorCommand::execute(){
 //report all otu values to file
 int IndicatorCommand::GetIndicatorSpecies(Tree*& T){
        try {
+               
                string thisOutputDir = outputDir;
                if (outputDir == "") {  thisOutputDir += m->hasPath(inputFileName);  }
                string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(inputFileName)) + "indicator.summary";
@@ -305,7 +350,16 @@ int IndicatorCommand::GetIndicatorSpecies(Tree*& T){
                
                ofstream out;
                m->openOutputFile(outputFileName, out);
-               out << "Node\tOTU#\tIndVal" << endl;
+               out.setf(ios::fixed, ios::floatfield); out.setf(ios::showpoint);
+               
+               int numBins = 0;
+               if (sharedfile != "") { numBins = lookup[0]->getNumBins(); }
+               else { numBins = lookupFloat[0]->getNumBins(); }
+               
+               //print headings
+               out << "TreeNode\t";
+               for (int i = 0; i < numBins; i++) { out << "OTU-" << (i+1) << '\t'; }
+               out << endl;
                
                string treeOutputDir = outputDir;
                if (outputDir == "") {  treeOutputDir += m->hasPath(treefile);  }
@@ -323,7 +377,7 @@ int IndicatorCommand::GetIndicatorSpecies(Tree*& T){
                
                //you need the distances to leaf to decide grouping below
                //this will also set branch lengths if the tree does not include them
-               map<int, float> distToLeaf = getLengthToLeaf(T);
+               map<int, float> distToRoot = getDistToRoot(T);
                        
                //for each node
                for (int i = T->getNumLeaves(); i < T->getNumNodes(); i++) {
@@ -339,21 +393,32 @@ int IndicatorCommand::GetIndicatorSpecies(Tree*& T){
                        if (sharedfile != "") {
                                vector< vector<SharedRAbundVector*> > groupings;
                                
-                               /*groupings.resize(1);
-                               groupings[0].push_back(lookup[0]);
-                               groupings[0].push_back(lookup[1]);
-                               groupings[0].push_back(lookup[2]);
-                               groupings[0].push_back(lookup[3]);
-                               groupings[0].push_back(lookup[4]);*/
-                               
                                //get nodes that will be a valid grouping
                                //you are valid if you are not one of my descendants
-                               //AND your distToLeaf is <= mine
-                               //AND your distToLeaf is >= my smallest childs
-                               //AND you were not added as part of a larger grouping
+                               //AND your distToRoot is >= mine
+                               //AND you were not added as part of a larger grouping. Largest nodes are added first.
+                               
                                set<string> groupsAlreadyAdded;
+                               //create a grouping with my grouping
+                               vector<SharedRAbundVector*> subset;
+                               int count = 0;
+                               int doneCount = nodeToDescendants[i].size();
+                               for (int k = 0; k < lookup.size(); k++) {
+                                       //is this descendant of i
+                                       if ((nodeToDescendants[i].count(lookup[k]->getGroup()) != 0)) { 
+                                               subset.push_back(lookup[k]);
+                                               groupsAlreadyAdded.insert(lookup[k]->getGroup());
+                                               count++;
+                                       }
+                                       if (count == doneCount) { break; } //quit once you get the rabunds for this grouping
+                               }
+                               if (subset.size() != 0) { groupings.push_back(subset); }
+                               
+                               
                                for (int j = (T->getNumNodes()-1); j >= 0; j--) {
-                                       if ((descendantNodes[i].count(j) == 0) && (distToLeaf[j] <= distToLeaf[i]) && ((distToLeaf[j] >= distToLeaf[T->tree[i].getLChild()]) || (distToLeaf[j] >= distToLeaf[T->tree[i].getRChild()]))) {
+       
+                                       
+                                       if ((descendantNodes[i].count(j) == 0) && (distToRoot[j] >= distToRoot[i])) { 
                                                vector<SharedRAbundVector*> subset;
                                                int count = 0;
                                                int doneCount = nodeToDescendants[j].size();
@@ -372,8 +437,8 @@ int IndicatorCommand::GetIndicatorSpecies(Tree*& T){
                                        }
                                }
                                
-                               if (groupsAlreadyAdded.size() != lookup.size()) { m->mothurOut("[ERROR]: could not make proper groupings."); m->mothurOutEndLine(); }
-                               
+                               if (groupsAlreadyAdded.size() != lookup.size()) {  m->mothurOut("[ERROR]: could not make proper groupings."); m->mothurOutEndLine(); }
+                                                               
                                indicatorValues = getValues(groupings);
                                
                        }else {
@@ -381,12 +446,27 @@ int IndicatorCommand::GetIndicatorSpecies(Tree*& T){
                                
                                //get nodes that will be a valid grouping
                                //you are valid if you are not one of my descendants
-                               //AND your distToLeaf is <= mine
-                               //AND your distToLeaf is >= my smallest childs
-                               //AND you were not added as part of a larger grouping
+                               //AND your distToRoot is >= mine
+                               //AND you were not added as part of a larger grouping. Largest nodes are added first.
+                               
                                set<string> groupsAlreadyAdded;
+                               //create a grouping with my grouping
+                               vector<SharedRAbundFloatVector*> subset;
+                               int count = 0;
+                               int doneCount = nodeToDescendants[i].size();
+                               for (int k = 0; k < lookupFloat.size(); k++) {
+                                       //is this descendant of i
+                                       if ((nodeToDescendants[i].count(lookupFloat[k]->getGroup()) != 0)) { 
+                                               subset.push_back(lookupFloat[k]);
+                                               groupsAlreadyAdded.insert(lookupFloat[k]->getGroup());
+                                               count++;
+                                       }
+                                       if (count == doneCount) { break; } //quit once you get the rabunds for this grouping
+                               }
+                               if (subset.size() != 0) { groupings.push_back(subset); }
+                               
                                for (int j = (T->getNumNodes()-1); j >= 0; j--) {
-                                       if ((descendantNodes[i].count(j) == 0) && (distToLeaf[j] <= distToLeaf[i]) && ((distToLeaf[j] >= distToLeaf[T->tree[i].getLChild()]) || (distToLeaf[j] >= distToLeaf[T->tree[i].getRChild()]))) {
+                                       if ((descendantNodes[i].count(j) == 0) && (distToRoot[j] >= distToRoot[i])) {
                                                vector<SharedRAbundFloatVector*> subset;
                                                int count = 0;
                                                int doneCount = nodeToDescendants[j].size();
@@ -412,30 +492,20 @@ int IndicatorCommand::GetIndicatorSpecies(Tree*& T){
                        
                        if (m->control_pressed) { out.close(); return 0; }
                        
+                       
                        /******************************************************/
                        //output indicator values to table form + label tree  //
                        /*****************************************************/
-                       vector<int> indicatorOTUs;
-                       float largestValue = 0.0;
+                       out << (i+1) << '\t';
                        for (int j = 0; j < indicatorValues.size(); j++) {
                                
                                if (m->control_pressed) { out.close(); return 0; }
                                
-                               out << (i+1) << '\t' << (j+1) << '\t' << indicatorValues[j] << endl;
-                               
-                               //show no favortism
-                               if (indicatorValues[j] > largestValue) { 
-                                       largestValue = indicatorValues[j];
-                                       indicatorOTUs.clear();
-                                       indicatorOTUs.push_back(j+1);
-                               }else if (indicatorValues[j] == largestValue) { 
-                                       indicatorOTUs.push_back(j+1);
-                               }
-                               
-                               random_shuffle(indicatorOTUs.begin(), indicatorOTUs.end());
-                               
-                               T->tree[i].setLabel(indicatorOTUs[0]);
+                               out << indicatorValues[j] << '\t';
                        }
+                       out << endl;
+                       
+                       T->tree[i].setLabel((i+1));
                        
                }
                out.close();
@@ -562,45 +632,60 @@ vector<float> IndicatorCommand::getValues(vector< vector<SharedRAbundVector*> >&
        }
 }
 //**********************************************************************************************************************
-//you need the distances to leaf to decide groupings
+//you need the distances to root to decide groupings
 //this will also set branch lengths if the tree does not include them
-map<int, float> IndicatorCommand::getLengthToLeaf(Tree*& T){
+map<int, float> IndicatorCommand::getDistToRoot(Tree*& T){
        try {
                map<int, float> dists;
                
-               for (int i = 0; i < T->getNumNodes(); i++) {
-                       
-                       int lc = T->tree[i].getLChild();
-                       int rc = T->tree[i].getRChild();
-                                
-                       //if you have no branch length, set it then calc
-                       if (T->tree[i].getBranchLength() <= 0.0) {
+               bool hasBranchLengths = false;
+               for (int i = 0; i < T->getNumNodes(); i++) { 
+                       if (T->tree[i].getBranchLength() > 0.0) {  hasBranchLengths = true; break; }
+               }
+               
+               //set branchlengths if needed
+               if (!hasBranchLengths) { 
+                       for (int i = 0; i < T->getNumNodes(); i++) {
+                               
+                               int lc = T->tree[i].getLChild();
+                               int rc = T->tree[i].getRChild();
                                
                                if (lc == -1) { // you are a leaf
                                        //if you are a leaf set you priliminary length to 1.0, this may adjust later
                                        T->tree[i].setBranchLength(1.0);
-                                       dists[i] = 0.0;
+                                       dists[i] = 1.0;
                                }else{ // you are an internal node
                                        //look at your children's length to leaf
                                        float ldist = dists[lc];
                                        float rdist = dists[rc];
                                        
-                                       float greater;
-                                       if (rdist > greater) { greater = rdist; }
-                                       else { greater = ldist; }
+                                       float greater = ldist;
+                                       if (rdist > greater) { greater = rdist; dists[i] = ldist + 1.0;}
+                                       else { dists[i] = rdist + 1.0; }
+                                       
                                        
                                        //branch length = difference + 1
                                        T->tree[lc].setBranchLength((abs(ldist-greater) + 1.0));
                                        T->tree[rc].setBranchLength((abs(rdist-greater) + 1.0));
-                                       
-                                       dists[i] = dists[lc] + (abs(ldist-greater) + 1.0);
                                }
-                               
-                       }else{
-                               if (lc == -1) { dists[i] = 0.0; }
-                               else { dists[i] = dists[lc] + T->tree[lc].getBranchLength(); }
+                       }
+               }
+                       
+               dists.clear();
+               
+               for (int i = 0; i < T->getNumNodes(); i++) {
+                       
+                       double sum = 0.0;
+                       int index = i;
+                       
+                       while(T->tree[index].getParent() != -1){
+                               if (T->tree[index].getBranchLength() != -1) {
+                                       sum += abs(T->tree[index].getBranchLength()); 
+                               }
+                               index = T->tree[index].getParent();
                        }
                        
+                       dists[i] = sum;
                }
                
                return dists;
@@ -622,8 +707,18 @@ set<string> IndicatorCommand::getDescendantList(Tree*& T, int i, map<int, set<st
                
                if (lc == -1) { //you are a leaf your only descendant is yourself
                        set<int> temp; temp.insert(i);
-                       names.insert(T->tree[i].getName());
                        nodes[i] = temp;
+                       
+                       if (designfile == "") {
+                               names.insert(T->tree[i].getName());
+                       }else {
+                               vector<string> myGroup; myGroup.push_back(T->tree[i].getName());
+                               vector<string> myReps = designMap->getNamesSeqs(myGroup);
+                               for (int k = 0; k < myReps.size(); k++) {
+                                       names.insert(myReps[k]);
+                               }
+                       }
+                       
                }else{ //your descedants are the combination of your childrens descendants
                        names = descendants[lc];
                        nodes[i] = nodes[lc];
@@ -633,6 +728,8 @@ set<string> IndicatorCommand::getDescendantList(Tree*& T, int i, map<int, set<st
                        for (set<int>::iterator itNum = nodes[rc].begin(); itNum != nodes[rc].end(); itNum++) {
                                nodes[i].insert(*itNum);
                        }
+                       //you are your own descendant
+                       nodes[i].insert(i);
                }
                
                return names;
@@ -649,6 +746,8 @@ int IndicatorCommand::getShared(){
                lookup = input->getSharedRAbundVectors();
                string lastLabel = lookup[0]->getLabel();
                
+               if (label == "") { label = lastLabel; delete input; return 0; }
+               
                //if the users enters label "0.06" and there is no "0.06" in their file use the next lowest label.
                set<string> labels; labels.insert(label);
                set<string> processedLabels;
@@ -723,6 +822,8 @@ int IndicatorCommand::getSharedFloat(){
                lookupFloat = input->getSharedRAbundFloatVectors();
                string lastLabel = lookupFloat[0]->getLabel();
                
+               if (label == "") { label = lastLabel; delete input; return 0; }
+               
                //if the users enters label "0.06" and there is no "0.06" in their file use the next lowest label.
                set<string> labels; labels.insert(label);
                set<string> processedLabels;