#include "heatmap.h"
//**********************************************************************************************************************
-HeatMap::HeatMap(){
+HeatMap::HeatMap(string sort, string scale, int num, int fsize, string dir, string i){
try {
- globaldata = GlobalData::getInstance();
- format = globaldata->getFormat();
+ m = MothurOut::getInstance();
+// format = globaldata->getFormat();
+ sorted = sort;
+ scaler = scale;
+ outputDir = dir;
+ numOTU = num;
+ fontSize = fsize;
+ inputfile = i;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "HeatMap", "HeatMap");
+ exit(1);
+ }
+}
+
+//**********************************************************************************************************************
+
+string HeatMap::getPic(RAbundVector* rabund) {
+ try {
+
+ int numBinsToDisplay = rabund->getNumBins();
+
+ if (numOTU != 0) { //user want to display a portion of the otus
+ if (numOTU < numBinsToDisplay) { numBinsToDisplay = numOTU; }
+ }
+
+ //sort lookup so shared bins are on top
+ if (sorted != "none") { sortRabund(rabund); }
+
+ float maxRelAbund = 0.0;
+
+ for(int i=0;i<rabund->size();i++){
+ float relAbund = rabund->get(i) / (float)rabund->getNumSeqs();
+ if(relAbund > maxRelAbund){ maxRelAbund = relAbund; }
+ }
+
+ vector<string> scaleRelAbund(numBinsToDisplay, "");
+
+ for(int i=0;i<numBinsToDisplay;i++){
+ float relAbund = rabund->get(i) / (float)rabund->getNumSeqs();
+
+ if (m->control_pressed) { return "control"; }
+
+ if (rabund->get(i) != 0) { //don't want log value of 0.
+ if (scaler == "log10") {
+ scaleRelAbund[i] = toHex(int(255 * log10(relAbund) / log10(maxRelAbund))) + "0000";
+ }else if (scaler == "log2") {
+ scaleRelAbund[i] = toHex(int(255 * log2(relAbund) / log2(maxRelAbund))) + "0000";
+ }else if (scaler == "linear") {
+ scaleRelAbund[i] = toHex(int(255 * relAbund / maxRelAbund)) + "0000";
+ }else { //if user enters invalid scaler option.
+ scaleRelAbund[i] = toHex(int(255 * log10(relAbund / log10(maxRelAbund)))) + "0000";
+ }
+ }
+ else { scaleRelAbund[i] = "FFFFFF"; }
+ }
+
+
+ string filenamesvg = outputDir + m->getRootName(m->getSimpleName(inputfile)) + rabund->getLabel() + ".heatmap.bin.svg";
+ m->openOutputFile(filenamesvg, outsvg);
+
+ //svg image
+ outsvg << "<svg xmlns:svg=\"http://www.w3.org/2000/svg\" xmlns=\"http://www.w3.org/2000/svg\" width=\"100%\" height=\"100%\" viewBox=\"0 0 " + toString(300) + " " + toString((numBinsToDisplay*5 + 120)) + "\">\n";
+ outsvg << "<g>\n";
+
+ //white backround
+ outsvg << "<rect fill=\"white\" stroke=\"white\" x=\"0\" y=\"0\" width=\"" + toString(300) + "\" height=\"" + toString((numBinsToDisplay*5 + 120)) + "\"/>";
+ outsvg << "<text fill=\"black\" class=\"seri\" text-anchor=\"middle\" font-size=\"" + toString(fontSize) + "\" x=\"" + toString((150) - 40) + "\" y=\"25\">Heatmap at distance " + rabund->getLabel() + "</text>\n";
+
+ //output legend and color labels
+ string color;
+ int x = 0;
+ int y = 103 + (numBinsToDisplay*5);
+ printLegend(y, maxRelAbund);
+
+ y = 70;
+
+ for (int i = 0; i < scaleRelAbund.size(); i++) {
+ if (m->control_pressed) { outsvg.close(); return "control"; }
+
+ outsvg << "<rect fill=\"#" + scaleRelAbund[i] + "\" stroke=\"#" + scaleRelAbund[i] + "\" x=\"" + toString(x) + "\" y=\"" + toString(y) + "\" width=\"300\" height=\"5\"/>\n";
+ y += 5;
+ }
- if (format != "list") { setGroups(); }
+ outsvg << "</g>\n</svg>\n";
+ outsvg.close();
+ return filenamesvg;
}
catch(exception& e) {
- cout << "Standard Error: " << e.what() << " has occurred in the HeatMap class Function HeatMap. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ m->errorOut(e, "HeatMap", "getPic");
exit(1);
}
- catch(...) {
- cout << "An unknown error has occurred in the HeatMap class function HeatMap. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+}
+
+//**********************************************************************************************************************
+
+string HeatMap::getPic(vector<SharedRAbundVector*> lookup) {
+ try {
+
+ int numBinsToDisplay = lookup[0]->size();
+
+ if (numOTU != 0) { //user want to display a portion of the otus
+ if (numOTU < numBinsToDisplay) { numBinsToDisplay = numOTU; }
+ }
+
+ //sort lookup so shared bins are on top
+ vector<string> sortedLabels = m->currentSharedBinLabels;
+ if (sorted != "none") { sortedLabels = sortSharedVectors(lookup); }
+
+ vector<vector<string> > scaleRelAbund;
+ vector<float> maxRelAbund(lookup[0]->size(), 0.0);
+ float superMaxRelAbund = 0;
+
+ for(int i = 0; i < lookup.size(); i++){
+ for(int j=0; j<lookup[i]->size(); j++){
+
+ float relAbund = lookup[i]->getAbundance(j) / (float)lookup[i]->getNumSeqs();
+ if(relAbund > maxRelAbund[i]){ maxRelAbund[i] = relAbund; }
+ }
+ if(maxRelAbund[i] > superMaxRelAbund){ superMaxRelAbund = maxRelAbund[i]; }
+ }
+
+ scaleRelAbund.resize(lookup.size());
+ for(int i=0;i<lookup.size();i++){
+ scaleRelAbund[i].assign(numBinsToDisplay, "");
+ for(int j=0;j<numBinsToDisplay;j++){
+ if (m->control_pressed) { return "control"; }
+ float relAbund = lookup[i]->getAbundance(j) / (float)lookup[i]->getNumSeqs();
+
+ if (lookup[i]->getAbundance(j) != 0) { //don't want log value of 0.
+ if (scaler == "log10") {
+ scaleRelAbund[i][j] = toHex(int(255 * log10(relAbund) / log10(maxRelAbund[i]))) + "0000";
+ }else if (scaler == "log2") {
+ scaleRelAbund[i][j] = toHex(int(255 * log2(relAbund) / log2(maxRelAbund[i]))) + "0000";
+ }else if (scaler == "linear") {
+ scaleRelAbund[i][j] = toHex(int(255 * relAbund / maxRelAbund[i])) + "0000";
+ }else { //if user enters invalid scaler option.
+ scaleRelAbund[i][j] = toHex(int(255 * log10(relAbund / log10(maxRelAbund[i])))) + "0000";
+ }
+ }else { scaleRelAbund[i][j] = "FFFFFF"; }
+
+ }
+ }
+
+ string filenamesvg = outputDir + m->getRootName(m->getSimpleName(inputfile)) + lookup[0]->getLabel() + ".heatmap.bin.svg";
+ m->openOutputFile(filenamesvg, outsvg);
+ int binHeight = 20;
+ int labelBump = 100;
+ int binWidth = 300;
+
+ //svg image
+ outsvg << "<svg xmlns:svg=\"http://www.w3.org/2000/svg\" xmlns=\"http://www.w3.org/2000/svg\" width=\"100%\" height=\"100%\" viewBox=\"0 0 " + toString(lookup.size() * binWidth + labelBump) + " " + toString((numBinsToDisplay*binHeight + 120)) + "\">\n";
+ outsvg << "<g>\n";
+
+ //white backround
+ outsvg << "<rect fill=\"white\" stroke=\"white\" x=\"0\" y=\"0\" width=\"" + toString(lookup.size() * binWidth+labelBump) + "\" height=\"" + toString((numBinsToDisplay*binHeight + 120)) + "\"/>";
+ outsvg << "<text fill=\"black\" class=\"seri\" font-size=\"" + toString(fontSize) + "\" text-anchor=\"middle\" x=\"" + toString((lookup.size() * 150) - 40) + "\" y=\"25\">Heatmap at distance " + lookup[0]->getLabel() + "</text>\n";
+
+ //column labels
+ for (int h = 0; h < lookup.size()+1; h++) {
+ if (h == 0) {
+ string tempLabel = "OTU";
+ outsvg << "<text fill=\"black\" class=\"seri\" font-size=\"" + toString(fontSize) + "\" x=\"" + toString(labelBump-labelBump/2+1) + "\" y=\"50\">" + tempLabel + "</text>\n";
+ }else {
+ outsvg << "<text fill=\"black\" class=\"seri\" font-size=\"" + toString(fontSize) + "\" x=\"" + toString(((binWidth * h) - 150) - ((int)lookup[h-1]->getGroup().length() / 2)+labelBump/2) + "\" y=\"50\">" + lookup[h-1]->getGroup() + "</text>\n";
+ }
+ }
+
+ //output legend and color labels
+ string color;
+ int x = 0;
+ int y = 103 + (numBinsToDisplay*binHeight);
+ printLegend(y, superMaxRelAbund);
+
+ y = 70;
+ for (int i = 0; i < numBinsToDisplay; i++) {
+ outsvg << "<text fill=\"black\" class=\"seri\" font-size=\"" + toString(fontSize) + "\" x=\"" + toString(x) + "\" y=\"" + toString(y) + "\">" + sortedLabels[i] + "</text>\n";
+ x += labelBump;
+ for (int j = 0; j < scaleRelAbund.size(); j++) {
+ if (m->control_pressed) { outsvg.close(); return "control"; }
+
+ outsvg << "<rect fill=\"#" + scaleRelAbund[j][i] + "\" stroke=\"#" + scaleRelAbund[j][i] + "\" x=\"" + toString(x) + "\" y=\"" + toString(y) + "\" width=\"" + toString(binWidth) + "\" height=\"" + toString(binHeight) + "\"/>\n";
+ x += binWidth;
+ }
+ x = 0;
+ y += binHeight;
+ }
+
+ outsvg << "</g>\n</svg>\n";
+ outsvg.close();
+
+ return filenamesvg;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "HeatMap", "getPic");
exit(1);
}
}
+
//**********************************************************************************************************************
-void HeatMap::getPic(OrderVector* order) {
+vector<string> HeatMap::sortSharedVectors(vector<SharedRAbundVector*>& lookup){
try {
- sabund = order->getSAbundVector();
- string filename = getRootName(globaldata->inputFileName) + "heatmap" + order->getLabel();
+
+ vector<SharedRAbundVector*> looktemp;
+ map<int, int> place; //spot in lookup where you insert shared by, ie, 3 -> 2 if they are shared by 3 inset into location 2.
+ map<int, int>::iterator it;
+
+ vector<string> sortedLabels = m->currentSharedBinLabels;
+
+ /****************** find order of otus **********************/
+ if (sorted == "shared") {
+ place = orderShared(lookup);
+ }else if (sorted == "topotu") {
+ place = orderTopOtu(lookup);
+ }else if (sorted == "topgroup") {
+ place = orderTopGroup(lookup);
+ }else { m->mothurOut("Error: invalid sort option."); m->mothurOutEndLine(); return sortedLabels; }
+
+
+ /******************* create copy of lookup *********************/
+ //create and initialize looktemp as a copy of lookup
+ for (int i = 0; i < lookup.size(); i++) {
+ SharedRAbundVector* temp = new SharedRAbundVector(lookup[i]->getNumBins());
+ temp->setLabel(lookup[i]->getLabel());
+ temp->setGroup(lookup[i]->getGroup());
+ //copy lookup i's info
+ for (int j = 0; j < lookup[i]->size(); j++) {
+ temp->set(j, lookup[i]->getAbundance(j), lookup[i]->getGroup());
+ }
+ looktemp.push_back(temp);
+ }
+
+ /************************ fill lookup in order given by place *********************/
+ //for each bin
+ for (int i = 0; i < looktemp[0]->size(); i++) { //place
+ //fill lookup // 2 -> 1
+ for (int j = 0; j < looktemp.size(); j++) { // 3 -> 2
+ int newAbund = looktemp[j]->getAbundance(i); // 1 -> 3
+ lookup[j]->set(place[i], newAbund, looktemp[j]->getGroup()); //binNumber, abundance, group
+ }
+ sortedLabels[place[i]] = m->currentSharedBinLabels[i];
+ }
+
+ //delete looktemp -- Sarah look at - this is causing segmentation faults
+ for (int j = 0; j < looktemp.size(); j++) {
+// delete looktemp[j];
+ }
+
+ return sortedLabels;
+
}
catch(exception& e) {
- cout << "Standard Error: " << e.what() << " has occurred in the HeatMap class Function getPic. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ m->errorOut(e, "HeatMap", "sortSharedVectors");
exit(1);
}
- catch(...) {
- cout << "An unknown error has occurred in the HeatMap class function getPic. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+}
+//**********************************************************************************************************************
+map<int, int> HeatMap::orderShared(vector<SharedRAbundVector*>& lookup){
+ try {
+
+ map<int, int> place; //spot in lookup where you insert shared by, ie, 3 -> 2 if they are shared by 3 inset into location 2.
+ map<int, int>::iterator it;
+
+ vector<int> sharedBins;
+ vector<int> uniqueBins;
+
+ //for each bin
+ for (int i = 0; i < lookup[0]->size(); i++) {
+ int count = 0;
+
+ //is this bin shared
+ for (int j = 0; j < lookup.size(); j++) { if (lookup[j]->getAbundance(i) != 0) { count++; } }
+
+ if (count < 2) { uniqueBins.push_back(i); }
+ else { sharedBins.push_back(i); }
+ }
+
+ //fill place
+ for (int i = 0; i < sharedBins.size(); i++) { place[sharedBins[i]] = i; }
+ for (int i = 0; i < uniqueBins.size(); i++) { place[uniqueBins[i]] = (sharedBins.size() + i); }
+
+ return place;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "HeatMap", "orderShared");
exit(1);
}
}
//**********************************************************************************************************************
-void HeatMap::getPic(SharedOrderVector* sharedorder) {
+map<int, int> HeatMap::orderTopOtu(vector<SharedRAbundVector*>& lookup){
try {
- //fills vector of sharedsabunds - lookup
- getSharedVectors(sharedorder);
+
+ map<int, int> place; //spot in lookup where you insert shared by, ie, 3 -> 2 if they are shared by 3 inset into location 2.
+ map<int, int>::iterator it;
- string filename = getRootName(globaldata->inputFileName) + "heatmap" + sharedorder->getLabel();
+ vector<binCount> totals;
+ //for each bin
+ for (int i = 0; i < lookup[0]->size(); i++) {
+ int total = 0;
+
+ for (int j = 0; j < lookup.size(); j++) { total += lookup[j]->getAbundance(i); }
+
+ binCount temp(i, total);
+
+ totals.push_back(temp);
+ }
+
+ sort(totals.begin(), totals.end(), comparebinCounts);
+
+ //fill place
+ for (int i = 0; i < totals.size(); i++) { place[totals[i].bin] = i; }
+
+ return place;
}
catch(exception& e) {
- cout << "Standard Error: " << e.what() << " has occurred in the HeatMap class Function getPic. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ m->errorOut(e, "HeatMap", "orderTopOtu");
exit(1);
}
- catch(...) {
- cout << "An unknown error has occurred in the HeatMap class function getPic. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+}
+//**********************************************************************************************************************
+map<int, int> HeatMap::orderTopGroup(vector<SharedRAbundVector*>& lookup){
+ try {
+
+ map<int, int> place; //spot in lookup where you insert shared by, ie, 3 -> 2 if they are shared by 3 inset into location 2.
+ map<int, int>::iterator it;
+
+ vector < vector<binCount> > totals; //totals[0] = bin totals for group 0, totals[1] = bin totals for group 1, ...
+ totals.resize(lookup.size());
+
+ //for each bin
+ for (int i = 0; i < lookup[0]->size(); i++) {
+ for (int j = 0; j < lookup.size(); j++) {
+ binCount temp(i, (lookup[j]->getAbundance(i)));
+ totals[j].push_back(temp);
+ }
+ }
+
+ for (int i = 0; i < totals.size(); i++) { sort(totals[i].begin(), totals[i].end(), comparebinCounts); }
+
+ //fill place
+ //grab the top otu for each group adding it if its not already added
+ int count = 0;
+ for (int i = 0; i < totals[0].size(); i++) {
+
+ for (int j = 0; j < totals.size(); j++) {
+ it = place.find(totals[j][i].bin);
+
+ if (it == place.end()) { //not added yet
+ place[totals[j][i].bin] = count;
+ count++;
+ }
+ }
+ }
+
+ return place;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "HeatMap", "orderTopGroup");
exit(1);
}
}
//**********************************************************************************************************************
-void HeatMap::getSharedVectors(SharedOrderVector* order){
+
+void HeatMap::printLegend(int y, float maxbin) {
try {
- lookup.clear();
-
- vector<SharedRAbundVector*> templookup;
-
- //create and initialize vector of sharedvectors, one for each group
- for (int i = 0; i < globaldata->Groups.size(); i++) {
- SharedRAbundVector* temp = new SharedRAbundVector(order->getMaxRank());
- temp->setLabel(order->getLabel());
- temp->setGroup(globaldata->Groups[i]);
- templookup.push_back(temp);
- }
-
- int numSeqs = order->size();
- //sample all the members
- for(int i=0;i<numSeqs;i++){
- //get first sample
- individual chosen = order->get(i);
- int abundance;
-
- //set info for sharedvector in chosens group
- for (int j = 0; j < templookup.size(); j++) {
- if (chosen.group == templookup[j]->getGroup()) {
- abundance = templookup[j]->getAbundance(chosen.bin);
- templookup[j]->set(chosen.bin, (abundance + 1), chosen.group);
- break;
- }
+
+ //output legend and color labels
+ //go through map and give each score a color value
+ string color;
+ int x = 10;
+
+ //prints legend
+ for (int i = 1; i < 255; i++) {
+ color = toHex(int((float)(i)));
+ outsvg << "<rect fill=\"#" + color + "0000\" stroke=\"#" + color + "0000\" x=\"" + toString(x) + "\" y=\"" + toString(y) + "\" width=\"1\" height=\"10\"/>\n";
+ x += 1;
+ }
+
+ //prints legend labels
+ x = 10;
+ for (int i = 1; i<=5; i++) {
+ float label;
+ if(scaler== "log10") { label = maxbin * log10(51*i) / log10(255); }
+ else if(scaler== "log2") { label = maxbin * log2(51*i) / log2(255); }
+ else if(scaler== "linear") { label = maxbin * 51 * i / 255; }
+ else { label = maxbin * log10(51*i) / log10(255); }
+ label = int(label * 1000 + 0.5);
+ label /= 1000.0;
+ string text = toString(label, 3);
+
+ outsvg << "<text fill=\"black\" class=\"seri\" x=\"" + toString(x) + "\" y=\"" + toString(y-3) + "\">" + text + "</text>\n";
+ x += 60;
+ }
+ }
+
+ catch(exception& e) {
+ m->errorOut(e, "HeatMap", "printLegend");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+
+string HeatMap::getPic(vector<SharedRAbundFloatVector*> lookup) {
+ try {
+
+ int numBinsToDisplay = lookup[0]->size();
+
+ if (numOTU != 0) { //user want to display a portion of the otus
+ if (numOTU < numBinsToDisplay) { numBinsToDisplay = numOTU; }
+ }
+
+ //sort lookup so shared bins are on top
+ vector<string> sortedLabels = m->currentSharedBinLabels;
+ if (sorted != "none") { sortedLabels = sortSharedVectors(lookup); }
+
+ vector<vector<string> > scaleRelAbund;
+ vector<float> maxRelAbund(lookup.size(), 0.0);
+ float superMaxRelAbund = 0;
+
+ for(int i = 0; i < lookup.size(); i++){
+ for(int j=0; j<lookup[i]->size(); j++){
+
+ float relAbund = lookup[i]->getAbundance(j);
+ if(relAbund > maxRelAbund[i]){ maxRelAbund[i] = relAbund; }
}
+ if(maxRelAbund[i] > superMaxRelAbund){ superMaxRelAbund = maxRelAbund[i]; }
+ }
+
+ scaleRelAbund.resize(lookup.size());
+ for(int i=0;i<lookup.size();i++){
+ scaleRelAbund[i].assign(numBinsToDisplay, "");
+ for(int j=0;j<numBinsToDisplay;j++){
+ if (m->control_pressed) { return "control"; }
+ float relAbund = lookup[i]->getAbundance(j);
+
+ if (lookup[i]->getAbundance(j) != 0) { //don't want log value of 0.
+ if (scaler == "log10") {
+ scaleRelAbund[i][j] = toHex(int(255 * log10(relAbund) / log10(maxRelAbund[i]))) + "0000";
+ }else if (scaler == "log2") {
+ scaleRelAbund[i][j] = toHex(int(255 * log2(relAbund) / log2(maxRelAbund[i]))) + "0000";
+ }else if (scaler == "linear") {
+ scaleRelAbund[i][j] = toHex(int(255 * relAbund / maxRelAbund[i])) + "0000";
+ }else { //if user enters invalid scaler option.
+ scaleRelAbund[i][j] = toHex(int(255 * log10(relAbund / log10(maxRelAbund[i])))) + "0000";
+ }
+ }else { scaleRelAbund[i][j] = "FFFFFF"; }
+
+ }
+ }
+
+ string filenamesvg = outputDir + m->getRootName(m->getSimpleName(inputfile)) + lookup[0]->getLabel() + ".heatmap.bin.svg";
+ m->openOutputFile(filenamesvg, outsvg);
+
+ int binHeight = 20;
+ int labelBump = 100;
+ int binWidth = 300;
+
+ //svg image
+ outsvg << "<svg xmlns:svg=\"http://www.w3.org/2000/svg\" xmlns=\"http://www.w3.org/2000/svg\" width=\"100%\" height=\"100%\" viewBox=\"0 0 " + toString(lookup.size() * binWidth + labelBump) + " " + toString((numBinsToDisplay*binHeight + 120)) + "\">\n";
+ outsvg << "<g>\n";
+
+ //white backround
+ outsvg << "<rect fill=\"white\" stroke=\"white\" x=\"0\" y=\"0\" width=\"" + toString(lookup.size() * binWidth+labelBump) + "\" height=\"" + toString((numBinsToDisplay*binHeight + 120)) + "\"/>";
+ outsvg << "<text fill=\"black\" class=\"seri\" font-size=\"" + toString(fontSize) + "\" text-anchor=\"middle\" x=\"" + toString((lookup.size() * 150) - 40) + "\" y=\"25\">Heatmap at distance " + lookup[0]->getLabel() + "</text>\n";
+
+ //column labels
+ for (int h = 0; h < lookup.size()+1; h++) {
+ if (h == 0) {
+ string tempLabel = "OTU";
+ outsvg << "<text fill=\"black\" class=\"seri\" font-size=\"" + toString(fontSize) + "\" x=\"" + toString(labelBump-labelBump/2+1) + "\" y=\"50\">" + tempLabel + "</text>\n";
+ }else {
+ outsvg << "<text fill=\"black\" class=\"seri\" font-size=\"" + toString(fontSize) + "\" x=\"" + toString(((binWidth * h) - 150) - ((int)lookup[h-1]->getGroup().length() / 2)+labelBump/2) + "\" y=\"50\">" + lookup[h-1]->getGroup() + "</text>\n";
+ }
}
+
+ //output legend and color labels
+ string color;
+ int x = 0;
+ int y = 103 + (numBinsToDisplay*binHeight);
+ printLegend(y, superMaxRelAbund);
- //convert templookups rabunds to lookups sabunds
- for (int j = 0; j < templookup.size(); j++) {
- lookup.push_back(templookup[j]->getSharedSAbundVector());
- delete templookup[j];
+ y = 70;
+ for (int i = 0; i < numBinsToDisplay; i++) {
+ outsvg << "<text fill=\"black\" class=\"seri\" font-size=\"" + toString(fontSize) + "\" x=\"" + toString(x) + "\" y=\"" + toString(y) + "\">" + sortedLabels[i] + "</text>\n";
+ x += labelBump;
+ for (int j = 0; j < scaleRelAbund.size(); j++) {
+ if (m->control_pressed) { outsvg.close(); return "control"; }
+
+ outsvg << "<rect fill=\"#" + scaleRelAbund[j][i] + "\" stroke=\"#" + scaleRelAbund[j][i] + "\" x=\"" + toString(x) + "\" y=\"" + toString(y) + "\" width=\"" + toString(binWidth) + "\" height=\"" + toString(binHeight) + "\"/>\n";
+ x += binWidth;
+ }
+ x = 0;
+ y += binHeight;
}
+ outsvg << "</g>\n</svg>\n";
+ outsvg.close();
+ return filenamesvg;
+
}
catch(exception& e) {
- cout << "Standard Error: " << e.what() << " has occurred in the HeatMap class Function getSharedVectors. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ m->errorOut(e, "HeatMap", "getPic");
exit(1);
}
- catch(...) {
- cout << "An unknown error has occurred in the HeatMap class function getSharedVectors. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+}
+//**********************************************************************************************************************
+vector<string> HeatMap::sortSharedVectors(vector<SharedRAbundFloatVector*>& lookup){
+ try {
+
+ vector<SharedRAbundFloatVector*> looktemp;
+ map<int, int> place; //spot in lookup where you insert shared by, ie, 3 -> 2 if they are shared by 3 inset into location 2.
+ map<int, int>::iterator it;
+
+ vector<string> sortedLabels = m->currentSharedBinLabels;
+
+ /****************** find order of otus **********************/
+ if (sorted == "shared") {
+ place = orderShared(lookup);
+ }else if (sorted == "topotu") {
+ place = orderTopOtu(lookup);
+ }else if (sorted == "topgroup") {
+ place = orderTopGroup(lookup);
+ }else { m->mothurOut("Error: invalid sort option."); m->mothurOutEndLine(); return sortedLabels; }
+
+
+ /******************* create copy of lookup *********************/
+ //create and initialize looktemp as a copy of lookup
+ for (int i = 0; i < lookup.size(); i++) {
+ SharedRAbundFloatVector* temp = new SharedRAbundFloatVector(lookup[i]->getNumBins());
+ temp->setLabel(lookup[i]->getLabel());
+ temp->setGroup(lookup[i]->getGroup());
+ //copy lookup i's info
+ for (int j = 0; j < lookup[i]->size(); j++) {
+ temp->set(j, lookup[i]->getAbundance(j), lookup[i]->getGroup());
+ }
+ looktemp.push_back(temp);
+ }
+
+ /************************ fill lookup in order given by place *********************/
+ //for each bin
+ for (int i = 0; i < looktemp[0]->size(); i++) { //place
+ //fill lookup // 2 -> 1
+ for (int j = 0; j < looktemp.size(); j++) { // 3 -> 2
+ float newAbund = looktemp[j]->getAbundance(i); // 1 -> 3
+ lookup[j]->set(place[i], newAbund, looktemp[j]->getGroup()); //binNumber, abundance, group
+ sortedLabels[place[i]] = m->currentSharedBinLabels[i];
+ }
+ }
+
+ //delete looktemp -- Sarah look at - this is causing segmentation faults
+ for (int j = 0; j < looktemp.size(); j++) {
+// delete looktemp[j];
+ }
+
+ return sortedLabels;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "HeatMap", "sortSharedVectors");
exit(1);
}
-
}
-
//**********************************************************************************************************************
-void HeatMap::setGroups() {
+int HeatMap::sortRabund(RAbundVector*& r){
try {
- //if the user has not entered specific groups to analyze then do them all
- if (globaldata->Groups.size() != 0) {
- if (globaldata->Groups[0] != "all") {
- //check that groups are valid
- for (int i = 0; i < globaldata->Groups.size(); i++) {
- if (globaldata->gGroupmap->isValidGroup(globaldata->Groups[i]) != true) {
- cout << globaldata->Groups[i] << " is not a valid group, and will be disregarded." << endl;
- // erase the invalid group from globaldata->Groups
- globaldata->Groups.erase(globaldata->Groups.begin()+i);
- }
- }
+ map<int, int> place; //spot in lookup where you insert shared by, ie, 3 -> 2 if they are shared by 3 inset into location 2.
+ map<int, int>::iterator it;
+
+ /****************** find order of otus **********************/
+ vector<binCount> totals;
+
+ //for each bin
+ for (int i = 0; i < r->getNumBins(); i++) {
+ binCount temp(i, r->get(i));
- //if the user only entered invalid groups
- if (globaldata->Groups.size() == 0) {
- cout << "When using the groups parameter you must have at least 1 valid groups. I will run the command using all the groups in your groupfile." << endl;
- for (int i = 0; i < globaldata->gGroupmap->namesOfGroups.size(); i++) {
- globaldata->Groups.push_back(globaldata->gGroupmap->namesOfGroups[i]);
- }
- }
- }else{//user has enter "all" and wants the default groups
- globaldata->Groups.clear();
- for (int i = 0; i < globaldata->gGroupmap->namesOfGroups.size(); i++) {
- globaldata->Groups.push_back(globaldata->gGroupmap->namesOfGroups[i]);
- }
- globaldata->setGroups("");
+ totals.push_back(temp);
+ }
+
+ sort(totals.begin(), totals.end(), comparebinCounts);
+
+ //fill place
+ for (int i = 0; i < totals.size(); i++) { place[totals[i].bin] = i; }
+
+ /******************* create copy of lookup *********************/
+ //create and initialize rtemp as a copy of r
+
+ RAbundVector* rtemp = new RAbundVector(r->getNumBins());
+ for (int i = 0; i < r->size(); i++) { rtemp->set(i, r->get(i)); }
+ rtemp->setLabel(r->getLabel());
+
+ /************************ fill lookup in order given by place *********************/
+ //for each bin
+ for (int i = 0; i < rtemp->size(); i++) { //place
+ //fill lookup // 2 -> 1
+ // 3 -> 2
+ int newAbund = rtemp->get(i); // 1 -> 3
+ r->set(place[i], newAbund); //binNumber, abundance
+ }
+
+ return 0;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "HeatMap", "sortRabund");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+map<int, int> HeatMap::orderShared(vector<SharedRAbundFloatVector*>& lookup){
+ try {
+
+ map<int, int> place; //spot in lookup where you insert shared by, ie, 3 -> 2 if they are shared by 3 inset into location 2.
+ map<int, int>::iterator it;
+
+ vector<int> sharedBins;
+ vector<int> uniqueBins;
+
+ //for each bin
+ for (int i = 0; i < lookup[0]->size(); i++) {
+ int count = 0;
+
+ //is this bin shared
+ for (int j = 0; j < lookup.size(); j++) { if (lookup[j]->getAbundance(i) != 0) { count++; } }
+
+ if (count < 2) { uniqueBins.push_back(i); }
+ else { sharedBins.push_back(i); }
+ }
+
+ //fill place
+ for (int i = 0; i < sharedBins.size(); i++) { place[sharedBins[i]] = i; }
+ for (int i = 0; i < uniqueBins.size(); i++) { place[uniqueBins[i]] = (sharedBins.size() + i); }
+
+ return place;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "HeatMap", "orderShared");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+map<int, int> HeatMap::orderTopOtu(vector<SharedRAbundFloatVector*>& lookup){
+ try {
+
+ map<int, int> place; //spot in lookup where you insert shared by, ie, 3 -> 2 if they are shared by 3 inset into location 2.
+ map<int, int>::iterator it;
+
+ vector<binCountFloat> totals;
+
+ //for each bin
+ for (int i = 0; i < lookup[0]->size(); i++) {
+ int total = 0;
+
+ for (int j = 0; j < lookup.size(); j++) { total += lookup[j]->getAbundance(i); }
+
+ binCountFloat temp(i, total);
+
+ totals.push_back(temp);
+ }
+
+ sort(totals.begin(), totals.end(), comparebinFloatCounts);
+
+ //fill place
+ for (int i = 0; i < totals.size(); i++) { place[totals[i].bin] = i; }
+
+ return place;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "HeatMap", "orderTopOtu");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+map<int, int> HeatMap::orderTopGroup(vector<SharedRAbundFloatVector*>& lookup){
+ try {
+
+ map<int, int> place; //spot in lookup where you insert shared by, ie, 3 -> 2 if they are shared by 3 inset into location 2.
+ map<int, int>::iterator it;
+
+ vector < vector<binCountFloat> > totals; //totals[0] = bin totals for group 0, totals[1] = bin totals for group 1, ...
+ totals.resize(lookup.size());
+
+ //for each bin
+ for (int i = 0; i < lookup[0]->size(); i++) {
+ for (int j = 0; j < lookup.size(); j++) {
+ binCountFloat temp(i, (lookup[j]->getAbundance(i)));
+ totals[j].push_back(temp);
}
- }else {
- for (int i = 0; i < globaldata->gGroupmap->namesOfGroups.size(); i++) {
- globaldata->Groups.push_back(globaldata->gGroupmap->namesOfGroups[i]);
+ }
+
+ for (int i = 0; i < totals.size(); i++) { sort(totals[i].begin(), totals[i].end(), comparebinFloatCounts); }
+
+ //fill place
+ //grab the top otu for each group adding it if its not already added
+ int count = 0;
+ for (int i = 0; i < totals[0].size(); i++) {
+
+ for (int j = 0; j < totals.size(); j++) {
+ it = place.find(totals[j][i].bin);
+
+ if (it == place.end()) { //not added yet
+ place[totals[j][i].bin] = count;
+ count++;
+ }
}
}
+
+ return place;
}
catch(exception& e) {
- cout << "Standard Error: " << e.what() << " has occurred in the HeatMap class Function setGroups. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
+ m->errorOut(e, "HeatMap", "orderTopGroup");
exit(1);
}
- catch(...) {
- cout << "An unknown error has occurred in the HeatMap class function setGroups. Please contact Pat Schloss at pschloss@microbio.umass.edu." << "\n";
- exit(1);
- }
-
}
-/***********************************************************/
+//**********************************************************************************************************************
+
+
+
+
+
projects / mothur.git / blobdiff