]> git.donarmstrong.com Git - mothur.git/blobdiff - getseqscommand.cpp
changes while testing
[mothur.git] / getseqscommand.cpp
index ffe49b48810d7916e5324efd9480c87e8322ec92..142023e7a40881c1c6397a761ba350132490b75c 100644 (file)
 #include "getseqscommand.h"
 #include "sequence.hpp"
 #include "listvector.hpp"
+#include "counttable.h"
 
 //**********************************************************************************************************************
 vector<string> GetSeqsCommand::setParameters(){        
        try {
-               CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "FNGLT", "none",false,false); parameters.push_back(pfasta);
-               CommandParameter pname("name", "InputTypes", "", "", "none", "FNGLT", "none",false,false); parameters.push_back(pname);
-               CommandParameter pgroup("group", "InputTypes", "", "", "none", "FNGLT", "none",false,false); parameters.push_back(pgroup);
-               CommandParameter plist("list", "InputTypes", "", "", "none", "FNGLT", "none",false,false); parameters.push_back(plist);
-               CommandParameter ptaxonomy("taxonomy", "InputTypes", "", "", "none", "FNGLT", "none",false,false); parameters.push_back(ptaxonomy);
-               CommandParameter palignreport("alignreport", "InputTypes", "", "", "none", "FNGLT", "none",false,false); parameters.push_back(palignreport);
-               CommandParameter pqfile("qfile", "InputTypes", "", "", "none", "FNGLT", "none",false,false); parameters.push_back(pqfile);
-               CommandParameter paccnos("accnos", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(paccnos);
-               CommandParameter pdups("dups", "Boolean", "", "T", "", "", "",false,false); parameters.push_back(pdups);
-               CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
-               CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir);
-               CommandParameter paccnos2("accnos2", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(paccnos2);
+               CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "FNGLT", "none","fasta",false,false,true); parameters.push_back(pfasta);
+        CommandParameter pname("name", "InputTypes", "", "", "NameCount", "FNGLT", "none","name",false,false,true); parameters.push_back(pname);
+        CommandParameter pcount("count", "InputTypes", "", "", "NameCount-CountGroup", "FNGLT", "none","count",false,false,true); parameters.push_back(pcount);
+               CommandParameter pgroup("group", "InputTypes", "", "", "CountGroup", "FNGLT", "none","group",false,false,true); parameters.push_back(pgroup);
+               CommandParameter plist("list", "InputTypes", "", "", "none", "FNGLT", "none","list",false,false,true); parameters.push_back(plist);
+               CommandParameter ptaxonomy("taxonomy", "InputTypes", "", "", "none", "FNGLT", "none","taxonomy",false,false,true); parameters.push_back(ptaxonomy);
+               CommandParameter palignreport("alignreport", "InputTypes", "", "", "none", "FNGLT", "none","alignreport",false,false); parameters.push_back(palignreport);
+               CommandParameter pqfile("qfile", "InputTypes", "", "", "none", "FNGLT", "none","qfile",false,false); parameters.push_back(pqfile);
+               CommandParameter paccnos("accnos", "InputTypes", "", "", "none", "none", "none","",false,true,true); parameters.push_back(paccnos);
+               CommandParameter pdups("dups", "Boolean", "", "T", "", "", "","",false,false); parameters.push_back(pdups);
+               CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
+               CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
+               CommandParameter paccnos2("accnos2", "InputTypes", "", "", "none", "none", "none","",false,false); parameters.push_back(paccnos2);
 
                vector<string> myArray;
                for (int i = 0; i < parameters.size(); i++) {   myArray.push_back(parameters[i].name);          }
@@ -40,7 +42,7 @@ vector<string> GetSeqsCommand::setParameters(){
 string GetSeqsCommand::getHelpString(){        
        try {
                string helpString = "";
-               helpString += "The get.seqs command reads an .accnos file and any of the following file types: fasta, name, group, list, taxonomy, quality or alignreport file.\n";
+               helpString += "The get.seqs command reads an .accnos file and any of the following file types: fasta, name, group, count, list, taxonomy, quality or alignreport file.\n";
                helpString += "It outputs a file containing only the sequences in the .accnos file.\n";
                helpString += "The get.seqs command parameters are accnos, fasta, name, group, list, taxonomy, qfile, alignreport and dups.  You must provide accnos unless you have a valid current accnos file, and at least one of the other parameters.\n";
                helpString += "The dups parameter allows you to add the entire line from a name file if you add any name from the line. default=false. \n";
@@ -68,6 +70,7 @@ GetSeqsCommand::GetSeqsCommand(){
                outputTypes["alignreport"] = tempOutNames;
                outputTypes["list"] = tempOutNames;
                outputTypes["qfile"] = tempOutNames;
+        outputTypes["count"] = tempOutNames;
                outputTypes["accnosreport"] = tempOutNames;
        }
        catch(exception& e) {
@@ -76,6 +79,29 @@ GetSeqsCommand::GetSeqsCommand(){
        }
 }
 //**********************************************************************************************************************
+string GetSeqsCommand::getOutputPattern(string type) {
+    try {
+        string pattern = "";
+        
+        if (type == "fasta")            {   pattern = "[filename],pick,[extension]";    }
+        else if (type == "taxonomy")    {   pattern = "[filename],pick,[extension]";    }
+        else if (type == "name")        {   pattern = "[filename],pick,[extension]";    }
+        else if (type == "group")       {   pattern = "[filename],pick,[extension]";    }
+        else if (type == "count")       {   pattern = "[filename],pick,[extension]";    }
+        else if (type == "list")        {   pattern = "[filename],pick,[extension]";    }
+        else if (type == "qfile")       {   pattern = "[filename],pick,[extension]";    }
+        else if (type == "accnosreport")      {   pattern = "[filename],pick.accnos.report";    }
+        else if (type == "alignreport")      {   pattern = "[filename],pick.align.report";    }
+        else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->control_pressed = true;  }
+        
+        return pattern;
+    }
+    catch(exception& e) {
+        m->errorOut(e, "GetSeqsCommand", "getOutputPattern");
+        exit(1);
+    }
+}
+//**********************************************************************************************************************
 GetSeqsCommand::GetSeqsCommand(string option)  {
        try {
                abort = false; calledHelp = false;   
@@ -108,6 +134,7 @@ GetSeqsCommand::GetSeqsCommand(string option)  {
                        outputTypes["list"] = tempOutNames;
                        outputTypes["qfile"] = tempOutNames;
                        outputTypes["accnosreport"] = tempOutNames;
+            outputTypes["count"] = tempOutNames;
                        
                        //if the user changes the output directory command factory will send this info to us in the output parameter 
                        outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){  outputDir = "";         }
@@ -188,6 +215,14 @@ GetSeqsCommand::GetSeqsCommand(string option)  {
                                        //if the user has not given a path then, add inputdir. else leave path alone.
                                        if (path == "") {       parameters["qfile"] = inputDir + it->second;            }
                                }
+                
+                it = parameters.find("count");
+                               //user has given a template file
+                               if(it != parameters.end()){ 
+                                       path = m->hasPath(it->second);
+                                       //if the user has not given a path then, add inputdir. else leave path alone.
+                                       if (path == "") {       parameters["count"] = inputDir + it->second;            }
+                               }
                        }
 
                        
@@ -206,12 +241,12 @@ GetSeqsCommand::GetSeqsCommand(string option)  {
                        if (accnosfile2 == "not found") { accnosfile2 = ""; }
                        
                        fastafile = validParameter.validFile(parameters, "fasta", true);
-                       if (fastafile == "not open") { abort = true; }
+                       if (fastafile == "not open") { fastafile = ""; abort = true; }
                        else if (fastafile == "not found") {  fastafile = "";  }
                        else { m->setFastaFile(fastafile); }
                        
                        namefile = validParameter.validFile(parameters, "name", true);
-                       if (namefile == "not open") { abort = true; }
+                       if (namefile == "not open") { namefile = ""; abort = true; }
                        else if (namefile == "not found") {  namefile = "";  }  
                        else { m->setNameFile(namefile); }
                        
@@ -230,7 +265,7 @@ GetSeqsCommand::GetSeqsCommand(string option)  {
                        else { m->setListFile(listfile); }
                        
                        taxfile = validParameter.validFile(parameters, "taxonomy", true);
-                       if (taxfile == "not open") { abort = true; }
+                       if (taxfile == "not open") { taxfile = ""; abort = true; }
                        else if (taxfile == "not found") {  taxfile = "";  }
                        else { m->setTaxonomyFile(taxfile); }
                        
@@ -243,12 +278,32 @@ GetSeqsCommand::GetSeqsCommand(string option)  {
                        if (accnosfile2 == "not open") { abort = true; }
                        else if (accnosfile2 == "not found") {  accnosfile2 = "";  }
                        
+            countfile = validParameter.validFile(parameters, "count", true);
+            if (countfile == "not open") { countfile = ""; abort = true; }
+            else if (countfile == "not found") { countfile = "";  }    
+            else { m->setCountTableFile(countfile); }
+            
+            if ((namefile != "") && (countfile != "")) {
+                m->mothurOut("[ERROR]: you may only use one of the following: name or count."); m->mothurOutEndLine(); abort = true;
+            }
+            
+            if ((groupfile != "") && (countfile != "")) {
+                m->mothurOut("[ERROR]: you may only use one of the following: group or count."); m->mothurOutEndLine(); abort=true;
+            }
+
                        
                        string usedDups = "true";
                        string temp = validParameter.validFile(parameters, "dups", false);      if (temp == "not found") { temp = "true"; usedDups = ""; }
                        dups = m->isTrue(temp);
                        
-                       if ((fastafile == "") && (namefile == "") && (groupfile == "") && (alignfile == "") && (listfile == "") && (taxfile == "") && (qualfile == "") && (accnosfile2 == ""))  { m->mothurOut("You must provide one of the following: fasta, name, group, alignreport, taxonomy, quality or listfile."); m->mothurOutEndLine(); abort = true; }
+                       if ((fastafile == "") && (namefile == "") && (groupfile == "") && (alignfile == "") && (listfile == "") && (taxfile == "") && (qualfile == "") && (accnosfile2 == "") && (countfile == ""))  { m->mothurOut("You must provide one of the following: fasta, name, group, count, alignreport, taxonomy, quality or listfile."); m->mothurOutEndLine(); abort = true; }
+            
+            if (countfile == "") {
+                if ((namefile == "") && ((fastafile != "") || (taxfile != ""))){
+                    vector<string> files; files.push_back(fastafile); files.push_back(taxfile);
+                    parser.getNameFile(files);
+                }
+            }
                }
 
        }
@@ -265,19 +320,28 @@ int GetSeqsCommand::execute(){
                if (abort == true) { if (calledHelp) { return 0; }  return 2;   }
                
                //get names you want to keep
-               readAccnos();
+               names = m->readAccnos(accnosfile);
                
                if (m->control_pressed) { return 0; }
+        
+        if (countfile != "") {
+            if ((fastafile != "") || (listfile != "") || (taxfile != "")) { 
+                m->mothurOut("\n[NOTE]: The count file should contain only unique names, so mothur assumes your fasta, list and taxonomy files also contain only uniques.\n\n");
+            }
+        }
                
                //read through the correct file and output lines you want to keep
                if (namefile != "")                     {               readName();                     }
                if (fastafile != "")            {               readFasta();            }
                if (groupfile != "")            {               readGroup();            }
+        if (countfile != "")           {               readCount();            }
                if (alignfile != "")            {               readAlign();            }
                if (listfile != "")                     {               readList();                     }
                if (taxfile != "")                      {               readTax();                      }
                if (qualfile != "")                     {               readQual();                     }
                if (accnosfile2 != "")          {               compareAccnos();        }
+        
+        if (m->debug) { runSanityCheck(); }
                
                if (m->control_pressed) { outputTypes.clear(); for (int i = 0; i < outputNames.size(); i++) {   m->mothurRemove(outputNames[i]);  } return 0; }
                
@@ -320,6 +384,10 @@ int GetSeqsCommand::execute(){
                                if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setQualFile(current); }
                        }
                        
+            itTypes = outputTypes.find("count");
+                       if (itTypes != outputTypes.end()) {
+                               if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setCountTableFile(current); }
+                       }
                }
                
                return 0;               
@@ -336,7 +404,10 @@ int GetSeqsCommand::readFasta(){
        try {
                string thisOutputDir = outputDir;
                if (outputDir == "") {  thisOutputDir += m->hasPath(fastafile);  }
-               string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(fastafile)) + "pick" +  m->getExtension(fastafile);
+               map<string, string> variables; 
+        variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(fastafile));
+        variables["[extension]"] = m->getExtension(fastafile);
+               string outputFileName = getOutputFileName("fasta", variables);
                ofstream out;
                m->openOutputFile(outputFileName, out);
                
@@ -347,6 +418,8 @@ int GetSeqsCommand::readFasta(){
                
                bool wroteSomething = false;
                int selectedCount = 0;
+        
+        if (m->debug) { set<string> temp; sanity["fasta"] = temp; }
                
                while(!in.eof()){
                
@@ -354,6 +427,11 @@ int GetSeqsCommand::readFasta(){
                        
                        Sequence currSeq(in);
                        name = currSeq.getName();
+            
+            if (!dups) {//adjust name if needed
+                map<string, string>::iterator it = uniqueMap.find(name);
+                if (it != uniqueMap.end()) { name = it->second; }
+            }
                        
                        if (name != "") {
                                //if this name is in the accnos file
@@ -362,6 +440,8 @@ int GetSeqsCommand::readFasta(){
                                        
                                        currSeq.printSequence(out);
                                        selectedCount++;
+                    
+                    if (m->debug) { sanity["fasta"].insert(name); }
                                }
                        }
                        m->gobble(in);
@@ -388,7 +468,10 @@ int GetSeqsCommand::readQual(){
        try {
                string thisOutputDir = outputDir;
                if (outputDir == "") {  thisOutputDir += m->hasPath(qualfile);  }
-               string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(qualfile)) + "pick" +  m->getExtension(qualfile);
+               map<string, string> variables; 
+        variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(qualfile));
+        variables["[extension]"] = m->getExtension(qualfile);
+               string outputFileName = getOutputFileName("qfile", variables);
                ofstream out;
                m->openOutputFile(outputFileName, out);
                
@@ -400,19 +483,25 @@ int GetSeqsCommand::readQual(){
                bool wroteSomething = false;
                int selectedCount = 0;
                
+        if (m->debug) { set<string> temp; sanity["qual"] = temp; }
                
                while(!in.eof()){       
                        string saveName = "";
                        string name = "";
                        string scores = "";
                        
-                       in >> name; 
+                       in >> name;
+            
+            if (!dups) {//adjust name if needed
+                map<string, string>::iterator it = uniqueMap.find(name);
+                if (it != uniqueMap.end()) { name = it->second; }
+            }
                                
                        if (name.length() != 0) { 
                                saveName = name.substr(1);
                                while (!in.eof())       {       
                                        char c = in.get(); 
-                                       if (c == 10 || c == 13){        break;  }
+                                       if (c == 10 || c == 13 || c == -1){     break;  }
                                        else { name += c; }     
                                } 
                                m->gobble(in);
@@ -431,6 +520,7 @@ int GetSeqsCommand::readQual(){
                                                
                                out << name << endl << scores;
                                selectedCount++;
+                if (m->debug) { sanity["qual"].insert(name); }
                        }
                        
                        m->gobble(in);
@@ -453,12 +543,76 @@ int GetSeqsCommand::readQual(){
                exit(1);
        }
 }
+//**********************************************************************************************************************
+int GetSeqsCommand::readCount(){
+       try {
+               string thisOutputDir = outputDir;
+               if (outputDir == "") {  thisOutputDir += m->hasPath(countfile);  }
+               map<string, string> variables; 
+               variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(countfile));
+        variables["[extension]"] = m->getExtension(countfile);
+               string outputFileName = getOutputFileName("count", variables);
+               
+               ofstream out;
+               m->openOutputFile(outputFileName, out);
+               
+               ifstream in;
+               m->openInputFile(countfile, in);
+               
+               bool wroteSomething = false;
+               int selectedCount = 0;
+               
+        string headers = m->getline(in); m->gobble(in);
+        out << headers << endl;
+        
+        string name, rest; int thisTotal;
+        while (!in.eof()) {
+            
+            if (m->control_pressed) { in.close();  out.close();  m->mothurRemove(outputFileName);  return 0; }
+            
+            in >> name; m->gobble(in); 
+            in >> thisTotal; m->gobble(in);
+            rest = m->getline(in); m->gobble(in);
+            if (m->debug) { m->mothurOut("[DEBUG]: " + name + '\t' + rest + "\n"); }
+            
+            if (names.count(name) != 0) {
+                out << name << '\t' << thisTotal << '\t' << rest << endl;
+                wroteSomething = true;
+                selectedCount+= thisTotal;
+            }
+        }
+        in.close();
+               out.close();
+        
+        //check for groups that have been eliminated
+        CountTable ct;
+        if (ct.testGroups(outputFileName)) {
+            ct.readTable(outputFileName, true);
+            ct.printTable(outputFileName);
+        }
+               
+               if (wroteSomething == false) {  m->mothurOut("Your file does not contain any sequence from the .accnos file."); m->mothurOutEndLine();  }
+               outputTypes["count"].push_back(outputFileName); outputNames.push_back(outputFileName);
+               
+               m->mothurOut("Selected " + toString(selectedCount) + " sequences from your count file."); m->mothurOutEndLine();
+        
+               return 0;
+       }
+       catch(exception& e) {
+               m->errorOut(e, "GetSeqsCommand", "readCount");
+               exit(1);
+       }
+}
+
 //**********************************************************************************************************************
 int GetSeqsCommand::readList(){
        try {
                string thisOutputDir = outputDir;
                if (outputDir == "") {  thisOutputDir += m->hasPath(listfile);  }
-               string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(listfile)) + "pick" +  m->getExtension(listfile);
+        map<string, string> variables; 
+               variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(listfile));
+        variables["[extension]"] = m->getExtension(listfile);
+               string outputFileName = getOutputFileName("list", variables);
                ofstream out;
                m->openOutputFile(outputFileName, out);
                
@@ -467,6 +621,8 @@ int GetSeqsCommand::readList(){
                
                bool wroteSomething = false;
                int selectedCount = 0;
+        
+        if (m->debug) { set<string> temp; sanity["list"] = temp; }
                
                while(!in.eof()){
                        
@@ -486,19 +642,16 @@ int GetSeqsCommand::readList(){
                        
                                //parse out names that are in accnos file
                                string binnames = list.get(i);
+                vector<string> bnames;
+                m->splitAtComma(binnames, bnames);
                                
                                string newNames = "";
-                               while (binnames.find_first_of(',') != -1) { 
-                                       string name = binnames.substr(0,binnames.find_first_of(','));
-                                       binnames = binnames.substr(binnames.find_first_of(',')+1, binnames.length());
-                                       
+                for (int i = 0; i < bnames.size(); i++) {
+                                       string name = bnames[i];
                                        //if that name is in the .accnos file, add it
-                                       if (names.count(name) != 0) {  newNames += name + ",";  selectedCount++; }
+                                       if (names.count(name) != 0) {  newNames += name + ",";  selectedCount++; if (m->debug) { sanity["list"].insert(name); } }
                                }
                        
-                               //get last name
-                               if (names.count(binnames) != 0) {  newNames += binnames + ",";  selectedCount++; }
-
                                //if there are names in this bin add to new list
                                if (newNames != "") { 
                                        newNames = newNames.substr(0, newNames.length()-1); //rip off extra comma
@@ -535,7 +688,10 @@ int GetSeqsCommand::readName(){
        try {
                string thisOutputDir = outputDir;
                if (outputDir == "") {  thisOutputDir += m->hasPath(namefile);  }
-               string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(namefile)) + "pick" +  m->getExtension(namefile);
+        map<string, string> variables; 
+               variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(namefile));
+        variables["[extension]"] = m->getExtension(namefile);
+               string outputFileName = getOutputFileName("name", variables);
                ofstream out;
                m->openOutputFile(outputFileName, out);
                
@@ -546,6 +702,9 @@ int GetSeqsCommand::readName(){
                
                bool wroteSomething = false;
                int selectedCount = 0;
+        
+        if (m->debug) { set<string> temp; sanity["name"] = temp; }
+        if (m->debug) { set<string> temp; sanity["dupname"] = temp; }
                
                while(!in.eof()){
                
@@ -564,14 +723,16 @@ int GetSeqsCommand::readName(){
                        for (int i = 0; i < parsedNames.size(); i++) {
                                if (names.count(parsedNames[i]) != 0) {
                                        validSecond.push_back(parsedNames[i]);
+                    if (m->debug) { sanity["dupname"].insert(parsedNames[i]); }
                                }
                        }
 
                        if ((dups) && (validSecond.size() != 0)) { //dups = true and we want to add someone, then add everyone
-                               for (int i = 0; i < parsedNames.size(); i++) {  names.insert(parsedNames[i]);  }
+                               for (int i = 0; i < parsedNames.size(); i++) {  names.insert(parsedNames[i]); if (m->debug) { sanity["dupname"].insert(parsedNames[i]); } }
                                out << firstCol << '\t' << hold << endl;
                                wroteSomething = true;
                                selectedCount += parsedNames.size();
+                if (m->debug) { sanity["name"].insert(firstCol); }
                        }else {
                                selectedCount += validSecond.size();
                                
@@ -585,6 +746,8 @@ int GetSeqsCommand::readName(){
                                        //you know you have at least one valid second since first column is valid
                                        for (int i = 0; i < validSecond.size()-1; i++) {  out << validSecond[i] << ',';  }
                                        out << validSecond[validSecond.size()-1] << endl;
+                    
+                    if (m->debug) { sanity["name"].insert(firstCol); }
                                        
                                
                                //make first name in set you come to first column and then add the remaining names to second column
@@ -595,10 +758,14 @@ int GetSeqsCommand::readName(){
                                                wroteSomething = true;
                                                
                                                out << validSecond[0] << '\t';
+                        //we are changing the unique name in the fasta file
+                        uniqueMap[firstCol] = validSecond[0];
                                        
                                                //you know you have at least one valid second since first column is valid
                                                for (int i = 0; i < validSecond.size()-1; i++) {  out << validSecond[i] << ',';  }
                                                out << validSecond[validSecond.size()-1] << endl;
+                        
+                        if (m->debug) { sanity["name"].insert(validSecond[0]); }
                                        }
                                }
                        }
@@ -626,7 +793,10 @@ int GetSeqsCommand::readGroup(){
        try {
                string thisOutputDir = outputDir;
                if (outputDir == "") {  thisOutputDir += m->hasPath(groupfile);  }
-               string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(groupfile)) + "pick" + m->getExtension(groupfile);
+               map<string, string> variables; 
+               variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(groupfile));
+        variables["[extension]"] = m->getExtension(groupfile);
+               string outputFileName = getOutputFileName("group", variables);
                ofstream out;
                m->openOutputFile(outputFileName, out);
                
@@ -637,6 +807,8 @@ int GetSeqsCommand::readGroup(){
                
                bool wroteSomething = false;
                int selectedCount = 0;
+        
+        if (m->debug) { set<string> temp; sanity["group"] = temp; }
                
                while(!in.eof()){
 
@@ -645,6 +817,7 @@ int GetSeqsCommand::readGroup(){
 
                        in >> name;                             //read from first column
                        in >> group;                    //read from second column
+            
                        
                        //if this name is in the accnos file
                        if (names.count(name) != 0) {
@@ -652,6 +825,8 @@ int GetSeqsCommand::readGroup(){
                                
                                out << name << '\t' << group << endl;
                                selectedCount++;
+                
+                if (m->debug) {  sanity["group"].insert(name); }
                        }
                                        
                        m->gobble(in);
@@ -678,7 +853,10 @@ int GetSeqsCommand::readTax(){
        try {
                string thisOutputDir = outputDir;
                if (outputDir == "") {  thisOutputDir += m->hasPath(taxfile);  }
-               string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(taxfile)) + "pick" + m->getExtension(taxfile);
+               map<string, string> variables; 
+               variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(taxfile));
+        variables["[extension]"] = m->getExtension(taxfile);
+               string outputFileName = getOutputFileName("taxonomy", variables);
                ofstream out;
                m->openOutputFile(outputFileName, out);
                
@@ -688,6 +866,8 @@ int GetSeqsCommand::readTax(){
                
                bool wroteSomething = false;
                int selectedCount = 0;
+        
+        if (m->debug) { set<string> temp; sanity["tax"] = temp; }
                
                while(!in.eof()){
 
@@ -695,6 +875,11 @@ int GetSeqsCommand::readTax(){
 
                        in >> name;                             //read from first column
                        in >> tax;                      //read from second column
+            
+            if (!dups) {//adjust name if needed
+                map<string, string>::iterator it = uniqueMap.find(name);
+                if (it != uniqueMap.end()) { name = it->second; }
+            }
                        
                        //if this name is in the accnos file
                        if (names.count(name) != 0) {
@@ -702,6 +887,8 @@ int GetSeqsCommand::readTax(){
                                
                                out << name << '\t' << tax << endl;
                                selectedCount++;
+                
+                if (m->debug) { sanity["tax"].insert(name); }
                        }
                                        
                        m->gobble(in);
@@ -728,7 +915,9 @@ int GetSeqsCommand::readAlign(){
        try {
                string thisOutputDir = outputDir;
                if (outputDir == "") {  thisOutputDir += m->hasPath(alignfile);  }
-               string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(alignfile)) + "pick.align.report";
+        map<string, string> variables; 
+               variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(alignfile));
+               string outputFileName = getOutputFileName("alignreport", variables);
                ofstream out;
                m->openOutputFile(outputFileName, out);
                
@@ -753,6 +942,11 @@ int GetSeqsCommand::readAlign(){
 
 
                        in >> name;                             //read from first column
+            
+            if (!dups) {//adjust name if needed
+                map<string, string>::iterator it = uniqueMap.find(name);
+                if (it != uniqueMap.end()) { name = it->second; }
+            }
                        
                        //if this name is in the accnos file
                        if (names.count(name) != 0) {
@@ -795,28 +989,95 @@ int GetSeqsCommand::readAlign(){
        }
 }
 //**********************************************************************************************************************
-
-int GetSeqsCommand::readAccnos(){
+//just looking at common mistakes. 
+int GetSeqsCommand::runSanityCheck(){
        try {
-               
-               ifstream in;
-               m->openInputFile(accnosfile, in);
-               string name;
-               
-               while(!in.eof()){
-                       in >> name;
-                                               
-                       names.insert(name);
-                       
-                       m->gobble(in);
-               }
-               in.close();     
-               
-               return 0;
+        string thisOutputDir = outputDir;
+        if (outputDir == "") {  thisOutputDir += m->hasPath(fastafile);  }
+        string filename = outputDir + "get.seqs.debug.report";
+        
+        ofstream out;
+               m->openOutputFile(filename, out); 
+
+       
+        //compare fasta, name, qual and taxonomy if given to make sure they contain the same seqs
+        if (fastafile != "") {
+            if (namefile != "") { //compare with fasta
+                if (sanity["fasta"] != sanity["name"]) { //create mismatch file
+                    createMisMatchFile(out, fastafile, namefile, sanity["fasta"], sanity["name"]);
+                }
+            }
+            if (qualfile != "") {
+                if (sanity["fasta"] != sanity["qual"]) { //create mismatch file
+                    createMisMatchFile(out, fastafile, qualfile, sanity["fasta"], sanity["qual"]);
+                }
+            }
+            if (taxfile != "") {
+                if (sanity["fasta"] != sanity["tax"]) { //create mismatch file
+                    createMisMatchFile(out, fastafile, taxfile, sanity["fasta"], sanity["tax"]);
+                }
+            }
+        }
+        
+        //compare dupnames, groups and list if given to make sure they match
+        if (namefile != "") {
+            if (groupfile != "") {
+                if (sanity["dupname"] != sanity["group"]) { //create mismatch file
+                    createMisMatchFile(out, namefile, groupfile, sanity["dupname"], sanity["group"]);
+                } 
+            }
+            if (listfile != "") {
+                if (sanity["dupname"] != sanity["list"]) { //create mismatch file
+                    createMisMatchFile(out, namefile, listfile, sanity["dupname"], sanity["list"]);
+                } 
+            }
+        }else{
 
+            if ((groupfile != "") && (fastafile != "")) {
+                if (sanity["fasta"] != sanity["group"]) { //create mismatch file
+                    createMisMatchFile(out, fastafile, groupfile, sanity["fasta"], sanity["group"]);
+                } 
+            }
+        }
+        
+        out.close();
+        
+        if (m->isBlank(filename)) { m->mothurRemove(filename); }
+        else { m->mothurOut("\n[DEBUG]: " + filename + " contains the file mismatches.\n");outputNames.push_back(filename); outputTypes["debug"].push_back(filename); }
+        
+        return 0;
+    }
+       catch(exception& e) {
+               m->errorOut(e, "GetSeqsCommand", "runSanityCheck");
+               exit(1);
        }
+}
+//**********************************************************************************************************************
+//just looking at common mistakes. 
+int GetSeqsCommand::createMisMatchFile(ofstream& out, string filename1, string filename2, set<string> set1, set<string> set2){
+       try {
+        out << "****************************************" << endl << endl;
+        out << "Names unique to " << filename1 << ":\n";
+        
+        //remove names in set1 that are also in set2
+        for (set<string>::iterator it = set1.begin(); it != set1.end();) {
+            string name = *it;
+            
+            if (set2.count(name) == 0)  { out << name << endl;  } //name unique to set1
+            else                        { set2.erase(name);     } //you are in both so erase 
+            set1.erase(it++);
+        }
+        
+        out << "\nNames unique to " << filename2 << ":\n";
+        //output results
+        for (set<string>::iterator it = set2.begin(); it != set2.end(); it++) {  out << *it << endl;  }
+        
+        out << "****************************************" << endl << endl;
+        
+        return 0;
+    }
        catch(exception& e) {
-               m->errorOut(e, "GetSeqsCommand", "readAccnos");
+               m->errorOut(e, "GetSeqsCommand", "runSanityCheck");
                exit(1);
        }
 }
@@ -827,7 +1088,10 @@ int GetSeqsCommand::compareAccnos(){
                
                string thisOutputDir = outputDir;
                if (outputDir == "") {  thisOutputDir += m->hasPath(accnosfile);  }
-               string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(accnosfile)) + "accnos.report";
+        map<string, string> variables; 
+               variables["[filename]"] = thisOutputDir + m->getRootName(m->getSimpleName(accnosfile));
+               string outputFileName = getOutputFileName("accnosreport", variables);
+               
                ofstream out;
                m->openOutputFile(outputFileName, out);
                
@@ -920,7 +1184,7 @@ int GetSeqsCommand::compareAccnos(){
                
        }
        catch(exception& e) {
-               m->errorOut(e, "GetSeqsCommand", "readAccnos");
+               m->errorOut(e, "GetSeqsCommand", "compareAccnos");
                exit(1);
        }
 }