#include "filterseqscommand.h"
#include "sequence.hpp"
-/**************************************************************************************/
-FilterSeqsCommand::FilterSeqsCommand(string option){
+//**********************************************************************************************************************
+vector<string> FilterSeqsCommand::setParameters(){
+ try {
+ CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "none", "none","fasta-filter",false,true, true); parameters.push_back(pfasta);
+ CommandParameter phard("hard", "InputTypes", "", "", "none", "none", "none","",false,false); parameters.push_back(phard);
+ CommandParameter ptrump("trump", "String", "", "*", "", "", "","",false,false, true); parameters.push_back(ptrump);
+ CommandParameter psoft("soft", "Number", "", "0", "", "", "","",false,false); parameters.push_back(psoft);
+ CommandParameter pvertical("vertical", "Boolean", "", "T", "", "", "","",false,false, true); parameters.push_back(pvertical);
+ CommandParameter pprocessors("processors", "Number", "", "1", "", "", "","",false,false, true); parameters.push_back(pprocessors);
+ CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
+ CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
+
+ vector<string> myArray;
+ for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); }
+ return myArray;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "FilterSeqsCommand", "setParameters");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+string FilterSeqsCommand::getHelpString(){
+ try {
+ string helpString = "";
+ helpString += "The filter.seqs command reads a file containing sequences and creates a .filter and .filter.fasta file.\n";
+ helpString += "The filter.seqs command parameters are fasta, trump, soft, hard, processors and vertical. \n";
+ helpString += "The fasta parameter is required, unless you have a valid current fasta file. You may enter several fasta files to build the filter from and filter, by separating their names with -'s.\n";
+ helpString += "For example: fasta=abrecovery.fasta-amazon.fasta \n";
+ helpString += "The trump option will remove a column if the trump character is found at that position in any sequence of the alignment. Default=*, meaning no trump. \n";
+ helpString += "A soft mask removes any column where the dominant base (i.e. A, T, G, C, or U) does not occur in at least a designated percentage of sequences. Default=0.\n";
+ helpString += "The hard parameter allows you to enter a file containing the filter you want to use.\n";
+ helpString += "The vertical parameter removes columns where all sequences contain a gap character. The default is T.\n";
+ helpString += "The processors parameter allows you to specify the number of processors to use. The default is 1.\n";
+ helpString += "The filter.seqs command should be in the following format: \n";
+ helpString += "filter.seqs(fasta=yourFastaFile, trump=yourTrump) \n";
+ helpString += "Example filter.seqs(fasta=abrecovery.fasta, trump=.).\n";
+ helpString += "Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFasta).\n";
+ return helpString;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "FilterSeqsCommand", "getHelpString");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+string FilterSeqsCommand::getOutputPattern(string type) {
+ try {
+ string pattern = "";
+
+ if (type == "fasta") { pattern = "[filename],filter.fasta"; }
+ else if (type == "filter") { pattern = "[filename],filter"; }
+ else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->control_pressed = true; }
+
+ return pattern;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "FilterSeqsCommand", "getOutputPattern");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+FilterSeqsCommand::FilterSeqsCommand(){
+ try {
+ abort = true; calledHelp = true;
+ setParameters();
+ vector<string> tempOutNames;
+ outputTypes["fasta"] = tempOutNames;
+ outputTypes["filter"] = tempOutNames;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "FilterSeqsCommand", "FilterSeqsCommand");
+ exit(1);
+ }
+}
+/**************************************************************************************/
+FilterSeqsCommand::FilterSeqsCommand(string option) {
try {
- abort = false;
+ abort = false; calledHelp = false;
+ filterFileName = "";
//allow user to run help
- if(option == "help") { help(); abort = true; }
+ if(option == "help") { help(); abort = true; calledHelp = true; }
+ else if(option == "citation") { citation(); abort = true; calledHelp = true;}
else {
- //valid paramters for this command
- string Array[] = {"fasta", "trump", "soft", "hard", "vertical"};
- vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
+ vector<string> myArray = setParameters();
OptionParser parser(option);
map<string,string> parameters = parser.getParameters();
- ValidParameters validParameter;
+ ValidParameters validParameter("filter.seqs");
+ map<string,string>::iterator it;
//check to make sure all parameters are valid for command
- for (map<string,string>::iterator it = parameters.begin(); it != parameters.end(); it++) {
+ for (it = parameters.begin(); it != parameters.end(); it++) {
if (validParameter.isValidParameter(it->first, myArray, it->second) != true) { abort = true; }
}
+ //initialize outputTypes
+ vector<string> tempOutNames;
+ outputTypes["fasta"] = tempOutNames;
+ outputTypes["filter"] = tempOutNames;
+
+ //if the user changes the input directory command factory will send this info to us in the output parameter
+ string inputDir = validParameter.validFile(parameters, "inputdir", false);
+ if (inputDir == "not found"){ inputDir = ""; }
+ else {
+ string path;
+ it = parameters.find("fasta");
+ //user has given a template file
+ if(it != parameters.end()){
+ path = m->hasPath(it->second);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { parameters["fasta"] = inputDir + it->second; }
+ }
+
+ it = parameters.find("hard");
+ //user has given a template file
+ if(it != parameters.end()){
+ path = m->hasPath(it->second);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { parameters["hard"] = inputDir + it->second; }
+ }
+ }
+
//check for required parameters
- fastafile = validParameter.validFile(parameters, "fasta", true);
- if (fastafile == "not found") { mothurOut("fasta is a required parameter for the filter.seqs command."); mothurOutEndLine(); abort = true; }
- else if (fastafile == "not open") { abort = true; }
+ fasta = validParameter.validFile(parameters, "fasta", false);
+ if (fasta == "not found") {
+ fasta = m->getFastaFile();
+ if (fasta != "") {
+ fastafileNames.push_back(fasta);
+ m->mothurOut("Using " + fasta + " as input file for the fasta parameter."); m->mothurOutEndLine();
+ string simpleName = m->getSimpleName(fasta);
+ filterFileName += simpleName.substr(0, simpleName.find_first_of('.'));
+ }
+ else { m->mothurOut("You have no current fastafile and the fasta parameter is required."); m->mothurOutEndLine(); abort = true; }
+ }
+ else {
+ m->splitAtDash(fasta, fastafileNames);
+
+ //go through files and make sure they are good, if not, then disregard them
+ for (int i = 0; i < fastafileNames.size(); i++) {
+
+ bool ignore = false;
+ if (fastafileNames[i] == "current") {
+ fastafileNames[i] = m->getFastaFile();
+ if (fastafileNames[i] != "") { m->mothurOut("Using " + fastafileNames[i] + " as input file for the fasta parameter where you had given current."); m->mothurOutEndLine(); }
+ else {
+ m->mothurOut("You have no current fastafile, ignoring current."); m->mothurOutEndLine(); ignore=true;
+ //erase from file list
+ fastafileNames.erase(fastafileNames.begin()+i);
+ i--;
+ }
+ }
+
+ if (!ignore) {
+ if (inputDir != "") {
+ string path = m->hasPath(fastafileNames[i]);
+ //if the user has not given a path then, add inputdir. else leave path alone.
+ if (path == "") { fastafileNames[i] = inputDir + fastafileNames[i]; }
+ }
+ ifstream in;
+ int ableToOpen = m->openInputFile(fastafileNames[i], in, "noerror");
+
+ //if you can't open it, try default location
+ if (ableToOpen == 1) {
+ if (m->getDefaultPath() != "") { //default path is set
+ string tryPath = m->getDefaultPath() + m->getSimpleName(fastafileNames[i]);
+ m->mothurOut("Unable to open " + fastafileNames[i] + ". Trying default " + tryPath); m->mothurOutEndLine();
+ ifstream in2;
+ ableToOpen = m->openInputFile(tryPath, in2, "noerror");
+ in2.close();
+ fastafileNames[i] = tryPath;
+ }
+ }
+
+ //if you can't open it, try default location
+ if (ableToOpen == 1) {
+ if (m->getOutputDir() != "") { //default path is set
+ string tryPath = m->getOutputDir() + m->getSimpleName(fastafileNames[i]);
+ m->mothurOut("Unable to open " + fastafileNames[i] + ". Trying output directory " + tryPath); m->mothurOutEndLine();
+ ifstream in2;
+ ableToOpen = m->openInputFile(tryPath, in2, "noerror");
+ in2.close();
+ fastafileNames[i] = tryPath;
+ }
+ }
+
+ in.close();
+
+ if (ableToOpen == 1) {
+ m->mothurOut("Unable to open " + fastafileNames[i] + ". It will be disregarded."); m->mothurOutEndLine();
+ //erase from file list
+ fastafileNames.erase(fastafileNames.begin()+i);
+ i--;
+ }else{
+ string simpleName = m->getSimpleName(fastafileNames[i]);
+ filterFileName += simpleName.substr(0, simpleName.find_first_of('.'));
+ m->setFastaFile(fastafileNames[i]);
+ }
+ in.close();
+ }
+ }
+
+ //make sure there is at least one valid file left
+ if (fastafileNames.size() == 0) { m->mothurOut("no valid files."); m->mothurOutEndLine(); abort = true; }
+ }
+
+ if (!abort) {
+ //if the user changes the output directory command factory will send this info to us in the output parameter
+ outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){
+ outputDir = "";
+ outputDir += m->hasPath(fastafileNames[0]); //if user entered a file with a path then preserve it
+ }
+ }
//check for optional parameter and set defaults
// ...at some point should added some additional type checking...
string temp;
+ hard = validParameter.validFile(parameters, "hard", true); if (hard == "not found") { hard = ""; }
+ else if (hard == "not open") { hard = ""; abort = true; }
+
temp = validParameter.validFile(parameters, "trump", false); if (temp == "not found") { temp = "*"; }
trump = temp[0];
temp = validParameter.validFile(parameters, "soft", false); if (temp == "not found") { soft = 0; }
else { soft = (float)atoi(temp.c_str()) / 100.0; }
- hard = validParameter.validFile(parameters, "hard", true); if (hard == "not found") { hard = ""; }
- else if (hard == "not open") { abort = true; }
+ temp = validParameter.validFile(parameters, "processors", false); if (temp == "not found"){ temp = m->getProcessors(); }
+ m->setProcessors(temp);
+ m->mothurConvert(temp, processors);
- vertical = validParameter.validFile(parameters, "vertical", false); if (vertical == "not found") { vertical = "T"; }
+ vertical = validParameter.validFile(parameters, "vertical", false);
+ if (vertical == "not found") {
+ if ((hard == "") && (trump == '*') && (soft == 0)) { vertical = "T"; } //you have not given a hard file or set the trump char.
+ else { vertical = "F"; }
+ }
numSeqs = 0;
+ }
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "FilterSeqsCommand", "FilterSeqsCommand");
+ exit(1);
+ }
+}
+/**************************************************************************************/
+
+int FilterSeqsCommand::execute() {
+ try {
+
+ if (abort == true) { if (calledHelp) { return 0; } return 2; }
+
+ ifstream inFASTA;
+ m->openInputFile(fastafileNames[0], inFASTA);
+
+ Sequence testSeq(inFASTA);
+ alignmentLength = testSeq.getAlignLength();
+ inFASTA.close();
+
+ ////////////create filter/////////////////
+ m->mothurOut("Creating Filter... "); m->mothurOutEndLine();
+
+ filter = createFilter();
+
+ m->mothurOutEndLine(); m->mothurOutEndLine();
+
+ if (m->control_pressed) { outputTypes.clear(); return 0; }
+
+ #ifdef USE_MPI
+ int pid;
+ MPI_Comm_rank(MPI_COMM_WORLD, &pid);
+
+ if (pid == 0) { //only one process should output the filter
+ #endif
+
+ ofstream outFilter;
+
+ //prevent giantic file name
+ map<string, string> variables;
+ variables["[filename]"] = outputDir + filterFileName + ".";
+ if (fastafileNames.size() > 3) { variables["[filename]"] = outputDir + "merge."; }
+ string filterFile = getOutputFileName("filter", variables);
+
+ m->openOutputFile(filterFile, outFilter);
+ outFilter << filter << endl;
+ outFilter.close();
+ outputNames.push_back(filterFile); outputTypes["filter"].push_back(filterFile);
+
+ #ifdef USE_MPI
+ }
+ #endif
+
+ ////////////run filter/////////////////
+
+ m->mothurOut("Running Filter... "); m->mothurOutEndLine();
+
+ filterSequences();
+
+ m->mothurOutEndLine(); m->mothurOutEndLine();
+
+ int filteredLength = 0;
+ for(int i=0;i<alignmentLength;i++){
+ if(filter[i] == '1'){ filteredLength++; }
+ }
+
+ if (m->control_pressed) { outputTypes.clear(); for(int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; }
+
+
+ m->mothurOutEndLine();
+ m->mothurOut("Length of filtered alignment: " + toString(filteredLength)); m->mothurOutEndLine();
+ m->mothurOut("Number of columns removed: " + toString((alignmentLength-filteredLength))); m->mothurOutEndLine();
+ m->mothurOut("Length of the original alignment: " + toString(alignmentLength)); m->mothurOutEndLine();
+ m->mothurOut("Number of sequences used to construct filter: " + toString(numSeqs)); m->mothurOutEndLine();
+
+ //set fasta file as new current fastafile
+ string current = "";
+ itTypes = outputTypes.find("fasta");
+ if (itTypes != outputTypes.end()) {
+ if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setFastaFile(current); }
+ }
+
+ m->mothurOutEndLine();
+ m->mothurOut("Output File Names: "); m->mothurOutEndLine();
+ for(int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i]); m->mothurOutEndLine(); }
+ m->mothurOutEndLine();
+
+ return 0;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "FilterSeqsCommand", "execute");
+ exit(1);
+ }
+}
+/**************************************************************************************/
+int FilterSeqsCommand::filterSequences() {
+ try {
+
+ numSeqs = 0;
+
+ for (int s = 0; s < fastafileNames.size(); s++) {
- if (abort == false) {
+ for (int i = 0; i < lines.size(); i++) { delete lines[i]; } lines.clear();
+
+ map<string, string> variables;
+ variables["[filename]"] = outputDir + m->getRootName(m->getSimpleName(fastafileNames[s]));
+ string filteredFasta = getOutputFileName("fasta", variables);
+#ifdef USE_MPI
+ int pid, numSeqsPerProcessor, num;
+ int tag = 2001;
+ vector<unsigned long long>MPIPos;
+
+ MPI_Status status;
+ MPI_Comm_size(MPI_COMM_WORLD, &processors); //set processors to the number of mpi processes running
+ MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
+
+ MPI_File outMPI;
+ MPI_File inMPI;
+ int outMode=MPI_MODE_CREATE|MPI_MODE_WRONLY;
+ int inMode=MPI_MODE_RDONLY;
+
+ char outFilename[1024];
+ strcpy(outFilename, filteredFasta.c_str());
- if (soft != 0) { F.setSoft(soft); }
- if (trump != '*') { F.setTrump(trump); }
+ char inFileName[1024];
+ strcpy(inFileName, fastafileNames[s].c_str());
+
+ MPI_File_open(MPI_COMM_WORLD, inFileName, inMode, MPI_INFO_NULL, &inMPI); //comm, filename, mode, info, filepointer
+ MPI_File_open(MPI_COMM_WORLD, outFilename, outMode, MPI_INFO_NULL, &outMPI);
+
+ if (m->control_pressed) { MPI_File_close(&inMPI); MPI_File_close(&outMPI); return 0; }
+
+ if (pid == 0) { //you are the root process
+
+ MPIPos = m->setFilePosFasta(fastafileNames[s], num); //fills MPIPos, returns numSeqs
+ numSeqs += num;
+
+ //send file positions to all processes
+ for(int i = 1; i < processors; i++) {
+ MPI_Send(&num, 1, MPI_INT, i, tag, MPI_COMM_WORLD);
+ MPI_Send(&MPIPos[0], (num+1), MPI_LONG, i, tag, MPI_COMM_WORLD);
+ }
+
+ //figure out how many sequences you have to do
+ numSeqsPerProcessor = num / processors;
+ int startIndex = pid * numSeqsPerProcessor;
+ if(pid == (processors - 1)){ numSeqsPerProcessor = num - pid * numSeqsPerProcessor; }
+
+
+ //do your part
+ driverMPIRun(startIndex, numSeqsPerProcessor, inMPI, outMPI, MPIPos);
+
+ if (m->control_pressed) { MPI_File_close(&inMPI); MPI_File_close(&outMPI); return 0; }
+
+ //wait on chidren
+ for(int i = 1; i < processors; i++) {
+ char buf[5];
+ MPI_Recv(buf, 5, MPI_CHAR, i, tag, MPI_COMM_WORLD, &status);
+ }
+
+ }else { //you are a child process
+ MPI_Recv(&num, 1, MPI_INT, 0, tag, MPI_COMM_WORLD, &status);
+ MPIPos.resize(num+1);
+ numSeqs += num;
+ MPI_Recv(&MPIPos[0], (num+1), MPI_LONG, 0, tag, MPI_COMM_WORLD, &status);
+
+ //figure out how many sequences you have to align
+ numSeqsPerProcessor = num / processors;
+ int startIndex = pid * numSeqsPerProcessor;
+ if(pid == (processors - 1)){ numSeqsPerProcessor = num - pid * numSeqsPerProcessor; }
+
+
+ //align your part
+ driverMPIRun(startIndex, numSeqsPerProcessor, inMPI, outMPI, MPIPos);
+
+ if (m->control_pressed) { MPI_File_close(&inMPI); MPI_File_close(&outMPI); return 0; }
+
+ char buf[5];
+ strcpy(buf, "done");
+
+ //tell parent you are done.
+ MPI_Send(buf, 5, MPI_CHAR, 0, tag, MPI_COMM_WORLD);
+ }
+
+ MPI_File_close(&outMPI);
+ MPI_File_close(&inMPI);
+ MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case
+
+#else
+
+ vector<unsigned long long> positions;
+ if (savedPositions.size() != 0) { positions = savedPositions[s]; }
+ else {
+#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
+ positions = m->divideFile(fastafileNames[s], processors);
+#else
+ if(processors != 1){
+ int numFastaSeqs = 0;
+ positions = m->setFilePosFasta(fastafileNames[s], numFastaSeqs);
+ if (positions.size() < processors) { processors = positions.size(); }
+ }
+#endif
+ }
+ #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
+ //vector<unsigned long long> positions = m->divideFile(fastafileNames[s], processors);
+ for (int i = 0; i < (positions.size()-1); i++) {
+ lines.push_back(new linePair(positions[i], positions[(i+1)]));
+ }
+
+ if(processors == 1){
+ int numFastaSeqs = driverRunFilter(filter, filteredFasta, fastafileNames[s], lines[0]);
+ numSeqs += numFastaSeqs;
+ }else{
+ int numFastaSeqs = createProcessesRunFilter(filter, fastafileNames[s], filteredFasta);
+ numSeqs += numFastaSeqs;
+ }
+
+ if (m->control_pressed) { return 1; }
+ #else
+ if(processors == 1){
+ lines.push_back(new linePair(0, 1000));
+ int numFastaSeqs = driverRunFilter(filter, filteredFasta, fastafileNames[s], lines[0]);
+ numSeqs += numFastaSeqs;
+ }else {
+ int numFastaSeqs = positions.size()-1;
+ //positions = m->setFilePosFasta(fastafileNames[s], numFastaSeqs);
+
+ //figure out how many sequences you have to process
+ int numSeqsPerProcessor = numFastaSeqs / processors;
+ for (int i = 0; i < processors; i++) {
+ int startIndex = i * numSeqsPerProcessor;
+ if(i == (processors - 1)){ numSeqsPerProcessor = numFastaSeqs - i * numSeqsPerProcessor; }
+ lines.push_back(new linePair(positions[startIndex], numSeqsPerProcessor));
+ }
+
+ numFastaSeqs = createProcessesRunFilter(filter, fastafileNames[s], filteredFasta);
+ numSeqs += numFastaSeqs;
+ }
+
+ if (m->control_pressed) { return 1; }
+ #endif
+#endif
+ outputNames.push_back(filteredFasta); outputTypes["fasta"].push_back(filteredFasta);
+ }
+
+ return 0;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "FilterSeqsCommand", "filterSequences");
+ exit(1);
+ }
+}
+#ifdef USE_MPI
+/**************************************************************************************/
+int FilterSeqsCommand::driverMPIRun(int start, int num, MPI_File& inMPI, MPI_File& outMPI, vector<unsigned long long>& MPIPos) {
+ try {
+ string outputString = "";
+ int count = 0;
+ MPI_Status status;
+
+ for(int i=0;i<num;i++){
+
+ if (m->control_pressed) { return 0; }
+
+ //read next sequence
+ int length = MPIPos[start+i+1] - MPIPos[start+i];
+ char* buf4 = new char[length];
+ MPI_File_read_at(inMPI, MPIPos[start+i], buf4, length, MPI_CHAR, &status);
+
+ string tempBuf = buf4;
+ if (tempBuf.length() > length) { tempBuf = tempBuf.substr(0, length); }
+ istringstream iss (tempBuf,istringstream::in);
+ delete buf4;
+
+ Sequence seq(iss); m->gobble(iss);
+
+ if (seq.getName() != "") {
+ string align = seq.getAligned();
+ string filterSeq = "";
+
+ for(int j=0;j<alignmentLength;j++){
+ if(filter[j] == '1'){
+ filterSeq += align[j];
+ }
+ }
+
+ count++;
+ outputString += ">" + seq.getName() + "\n" + filterSeq + "\n";
+
+ if(count % 10 == 0){ //output to file
+ //send results to parent
+ int length = outputString.length();
+ char* buf = new char[length];
+ memcpy(buf, outputString.c_str(), length);
+
+ MPI_File_write_shared(outMPI, buf, length, MPI_CHAR, &status);
+ outputString = "";
+ delete buf;
+ }
+
}
-
+
+ if((i+1) % 100 == 0){ cout << (i+1) << endl; }
}
+ if(outputString != ""){ //output to file
+ //send results to parent
+ int length = outputString.length();
+ char* buf = new char[length];
+ memcpy(buf, outputString.c_str(), length);
+
+ MPI_File_write_shared(outMPI, buf, length, MPI_CHAR, &status);
+ outputString = "";
+ delete buf;
+ }
+
+ if((num) % 100 != 0){ cout << (num) << endl; }
+
+ return 0;
}
catch(exception& e) {
- errorOut(e, "FilterSeqsCommand", "FilterSeqsCommand");
+ m->errorOut(e, "FilterSeqsCommand", "driverRunFilter");
exit(1);
}
}
+#endif
+/**************************************************************************************/
+int FilterSeqsCommand::driverRunFilter(string F, string outputFilename, string inputFilename, linePair* filePos) {
+ try {
+ ofstream out;
+ m->openOutputFile(outputFilename, out);
+
+ ifstream in;
+ m->openInputFile(inputFilename, in);
+
+ in.seekg(filePos->start);
-//**********************************************************************************************************************
+ bool done = false;
+ int count = 0;
+
+ while (!done) {
+
+ if (m->control_pressed) { in.close(); out.close(); return 0; }
+
+ Sequence seq(in); m->gobble(in);
+ if (seq.getName() != "") {
+ string align = seq.getAligned();
+ string filterSeq = "";
+
+ for(int j=0;j<alignmentLength;j++){
+ if(filter[j] == '1'){
+ filterSeq += align[j];
+ }
+ }
+
+ out << '>' << seq.getName() << endl << filterSeq << endl;
+ count++;
+ }
+
+ #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
+ unsigned long long pos = in.tellg();
+ if ((pos == -1) || (pos >= filePos->end)) { break; }
+ #else
+ if (in.eof()) { break; }
+ #endif
+
+ //report progress
+ if((count) % 100 == 0){ m->mothurOutJustToScreen(toString(count)+"\n"); }
+ }
+ //report progress
+ if((count) % 100 != 0){ m->mothurOutJustToScreen(toString(count)+"\n"); }
+
+
+ out.close();
+ in.close();
+
+ return count;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "FilterSeqsCommand", "driverRunFilter");
+ exit(1);
+ }
+}
+/**************************************************************************************************/
-void FilterSeqsCommand::help(){
+int FilterSeqsCommand::createProcessesRunFilter(string F, string filename, string filteredFastaName) {
try {
- mothurOut("The filter.seqs command reads a file containing sequences and creates a .filter and .filter.fasta file.\n");
- mothurOut("The filter.seqs command parameters are fasta, trump, soft, hard and vertical. \n");
- mothurOut("The fasta parameter is required.\n");
- mothurOut("The trump parameter .... The default is ...\n");
- mothurOut("The soft parameter .... The default is ....\n");
- mothurOut("The hard parameter .... The default is ....\n");
- mothurOut("The vertical parameter .... The default is T.\n");
- mothurOut("The filter.seqs command should be in the following format: \n");
- mothurOut("filter.seqs(fasta=yourFastaFile, trump=yourTrump, soft=yourSoft, hard=yourHard, vertical=yourVertical) \n");
- mothurOut("Example filter.seqs(fasta=abrecovery.fasta, trump=..., soft=..., hard=..., vertical=T).\n");
- mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFasta).\n\n");
+
+ int process = 1;
+ int num = 0;
+ processIDS.clear();
+
+#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
+
+
+ //loop through and create all the processes you want
+ while (process != processors) {
+ int pid = fork();
+
+ if (pid > 0) {
+ processIDS.push_back(pid); //create map from line number to pid so you can append files in correct order later
+ process++;
+ }else if (pid == 0){
+ string filteredFasta = filename + toString(getpid()) + ".temp";
+ num = driverRunFilter(F, filteredFasta, filename, lines[process]);
+
+ //pass numSeqs to parent
+ ofstream out;
+ string tempFile = filename + toString(getpid()) + ".num.temp";
+ m->openOutputFile(tempFile, out);
+ out << num << endl;
+ out.close();
+
+ exit(0);
+ }else {
+ m->mothurOut("[ERROR]: unable to spawn the necessary processes."); m->mothurOutEndLine();
+ for (int i = 0; i < processIDS.size(); i++) { kill (processIDS[i], SIGINT); }
+ exit(0);
+ }
+ }
+
+ num = driverRunFilter(F, filteredFastaName, filename, lines[0]);
+
+ //force parent to wait until all the processes are done
+ for (int i=0;i<processIDS.size();i++) {
+ int temp = processIDS[i];
+ wait(&temp);
+ }
+
+ for (int i = 0; i < processIDS.size(); i++) {
+ ifstream in;
+ string tempFile = filename + toString(processIDS[i]) + ".num.temp";
+ m->openInputFile(tempFile, in);
+ if (!in.eof()) { int tempNum = 0; in >> tempNum; num += tempNum; }
+ in.close(); m->mothurRemove(tempFile);
+
+ m->appendFiles((filename + toString(processIDS[i]) + ".temp"), filteredFastaName);
+ m->mothurRemove((filename + toString(processIDS[i]) + ".temp"));
+ }
+
+#else
+
+ //////////////////////////////////////////////////////////////////////////////////////////////////////
+ //Windows version shared memory, so be careful when passing variables through the filterData struct.
+ //Above fork() will clone, so memory is separate, but that's not the case with windows,
+ //Taking advantage of shared memory to allow both threads to add info to F.
+ //////////////////////////////////////////////////////////////////////////////////////////////////////
+
+ vector<filterRunData*> pDataArray;
+ DWORD dwThreadIdArray[processors-1];
+ HANDLE hThreadArray[processors-1];
+ //Create processor worker threads.
+ for( int i=0; i<processors-1; i++){
+
+ string extension = "";
+ if (i != 0) { extension = toString(i) + ".temp"; }
+
+ filterRunData* tempFilter = new filterRunData(filter, filename, (filteredFastaName + extension), m, lines[i]->start, lines[i]->end, alignmentLength, i);
+ pDataArray.push_back(tempFilter);
+ processIDS.push_back(i);
+
+ hThreadArray[i] = CreateThread(NULL, 0, MyRunFilterThreadFunction, pDataArray[i], 0, &dwThreadIdArray[i]);
+ }
+
+ num = driverRunFilter(F, (filteredFastaName + toString(processors-1) + ".temp"), filename, lines[processors-1]);
+
+ //Wait until all threads have terminated.
+ WaitForMultipleObjects(processors-1, hThreadArray, TRUE, INFINITE);
+
+ //Close all thread handles and free memory allocations.
+ for(int i=0; i < pDataArray.size(); i++){
+ num += pDataArray[i]->count;
+ if (pDataArray[i]->count != pDataArray[i]->end) {
+ m->mothurOut("[ERROR]: process " + toString(i) + " only processed " + toString(pDataArray[i]->count) + " of " + toString(pDataArray[i]->end) + " sequences assigned to it, quitting. \n"); m->control_pressed = true;
+ }
+ CloseHandle(hThreadArray[i]);
+ delete pDataArray[i];
+ }
+
+ for (int i = 1; i < processors; i++) {
+ m->appendFiles((filteredFastaName + toString(i) + ".temp"), filteredFastaName);
+ m->mothurRemove((filteredFastaName + toString(i) + ".temp"));
+ }
+#endif
+
+ return num;
+
}
catch(exception& e) {
- errorOut(e, "FilterSeqsCommand", "help");
+ m->errorOut(e, "FilterSeqsCommand", "createProcessesRunFilter");
exit(1);
}
}
-
/**************************************************************************************/
-
-int FilterSeqsCommand::execute() {
+string FilterSeqsCommand::createFilter() {
try {
-
- if (abort == true) { return 0; }
-
- ifstream inFASTA;
- openInputFile(fastafile, inFASTA);
+ string filterString = "";
+ Filters F;
- Sequence testSeq(inFASTA);
- alignmentLength = testSeq.getAlignLength();
- inFASTA.seekg(0);
+ if (soft != 0) { F.setSoft(soft); }
+ if (trump != '*') { F.setTrump(trump); }
F.setLength(alignmentLength);
- if(soft != 0 || isTrue(vertical)){
+ if(trump != '*' || m->isTrue(vertical) || soft != 0){
F.initialize();
}
if(hard.compare("") != 0) { F.doHard(hard); }
else { F.setFilter(string(alignmentLength, '1')); }
-
- if(trump != '*' || isTrue(vertical) || soft != 0){
- while(!inFASTA.eof()){ //read through and create the filter...
- Sequence seq(inFASTA);
- if(trump != '*'){ F.doTrump(seq); }
- if(isTrue(vertical) || soft != 0){ F.getFreqs(seq); }
- numSeqs++;
- cout.flush();
+
+ numSeqs = 0;
+ if(trump != '*' || m->isTrue(vertical) || soft != 0){
+ for (int s = 0; s < fastafileNames.size(); s++) {
+
+ for (int i = 0; i < lines.size(); i++) { delete lines[i]; } lines.clear();
+
+#ifdef USE_MPI
+ int pid, numSeqsPerProcessor, num;
+ int tag = 2001;
+ vector<unsigned long long> MPIPos;
+
+ MPI_Status status;
+ MPI_File inMPI;
+ MPI_Comm_size(MPI_COMM_WORLD, &processors);
+ MPI_Comm_rank(MPI_COMM_WORLD, &pid);
+
+ //char* tempFileName = new char(fastafileNames[s].length());
+ //tempFileName = &(fastafileNames[s][0]);
+
+ char tempFileName[1024];
+ strcpy(tempFileName, fastafileNames[s].c_str());
+
+ MPI_File_open(MPI_COMM_WORLD, tempFileName, MPI_MODE_RDONLY, MPI_INFO_NULL, &inMPI); //comm, filename, mode, info, filepointer
+
+ if (m->control_pressed) { MPI_File_close(&inMPI); return 0; }
+
+ if (pid == 0) { //you are the root process
+ MPIPos = m->setFilePosFasta(fastafileNames[s], num); //fills MPIPos, returns numSeqs
+ numSeqs += num;
+
+ //send file positions to all processes
+ for(int i = 1; i < processors; i++) {
+ MPI_Send(&num, 1, MPI_INT, i, tag, MPI_COMM_WORLD);
+ MPI_Send(&MPIPos[0], (num+1), MPI_LONG, i, tag, MPI_COMM_WORLD);
+ }
+
+ //figure out how many sequences you have to do
+ numSeqsPerProcessor = num / processors;
+ int startIndex = pid * numSeqsPerProcessor;
+ if(pid == (processors - 1)){ numSeqsPerProcessor = num - pid * numSeqsPerProcessor; }
+
+
+ //do your part
+ MPICreateFilter(startIndex, numSeqsPerProcessor, F, inMPI, MPIPos);
+
+ if (m->control_pressed) { MPI_File_close(&inMPI); return 0; }
+
+ }else { //i am the child process
+ MPI_Recv(&num, 1, MPI_INT, 0, tag, MPI_COMM_WORLD, &status);
+ MPIPos.resize(num+1);
+ numSeqs += num;
+ MPI_Recv(&MPIPos[0], (num+1), MPI_LONG, 0, tag, MPI_COMM_WORLD, &status);
+
+ //figure out how many sequences you have to align
+ numSeqsPerProcessor = num / processors;
+ int startIndex = pid * numSeqsPerProcessor;
+ if(pid == (processors - 1)){ numSeqsPerProcessor = num - pid * numSeqsPerProcessor; }
+
+
+ //do your part
+ MPICreateFilter(startIndex, numSeqsPerProcessor, F, inMPI, MPIPos);
+
+ if (m->control_pressed) { MPI_File_close(&inMPI); return 0; }
+ }
+
+ MPI_File_close(&inMPI);
+ MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case
+
+#else
+
+ vector<unsigned long long> positions;
+ #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
+ positions = m->divideFile(fastafileNames[s], processors);
+ for (int i = 0; i < (positions.size()-1); i++) {
+ lines.push_back(new linePair(positions[i], positions[(i+1)]));
+ }
+
+ if(processors == 1){
+ int numFastaSeqs = driverCreateFilter(F, fastafileNames[s], lines[0]);
+ numSeqs += numFastaSeqs;
+ }else{
+ int numFastaSeqs = createProcessesCreateFilter(F, fastafileNames[s]);
+ numSeqs += numFastaSeqs;
+ }
+ #else
+ if(processors == 1){
+ lines.push_back(new linePair(0, 1000));
+ int numFastaSeqs = driverCreateFilter(F, fastafileNames[s], lines[0]);
+ numSeqs += numFastaSeqs;
+ }else {
+ int numFastaSeqs = 0;
+ positions = m->setFilePosFasta(fastafileNames[s], numFastaSeqs);
+ if (positions.size() < processors) { processors = positions.size(); }
+
+ //figure out how many sequences you have to process
+ int numSeqsPerProcessor = numFastaSeqs / processors;
+ for (int i = 0; i < processors; i++) {
+ int startIndex = i * numSeqsPerProcessor;
+ if(i == (processors - 1)){ numSeqsPerProcessor = numFastaSeqs - i * numSeqsPerProcessor; }
+ lines.push_back(new linePair(positions[startIndex], numSeqsPerProcessor));
+ }
+
+ numFastaSeqs = createProcessesCreateFilter(F, fastafileNames[s]);
+ numSeqs += numFastaSeqs;
+ }
+ #endif
+ //save the file positions so we can reuse them in the runFilter function
+ savedPositions[s] = positions;
+
+ if (m->control_pressed) { return filterString; }
+#endif
+
}
+ }
+
+
+#ifdef USE_MPI
+ int pid;
+ int Atag = 1; int Ttag = 2; int Ctag = 3; int Gtag = 4; int Gaptag = 5;
+ MPI_Status status;
+
+ MPI_Comm_rank(MPI_COMM_WORLD, &pid);
+ if(trump != '*' || m->isTrue(vertical) || soft != 0){
+
+ if (pid == 0) { //only one process should output the filter
+
+ vector<int> temp; temp.resize(alignmentLength+1);
+
+ //get the frequencies from the child processes
+ for(int i = 1; i < processors; i++) {
+
+ for (int j = 0; j < 5; j++) {
+
+ MPI_Recv(&temp[0], (alignmentLength+1), MPI_INT, i, 2001, MPI_COMM_WORLD, &status);
+ int receiveTag = temp[temp.size()-1]; //child process added a int to the end to indicate what letter count this is for
+
+ if (receiveTag == Atag) { //you are recieveing the A frequencies
+ for (int k = 0; k < alignmentLength; k++) { F.a[k] += temp[k]; }
+ }else if (receiveTag == Ttag) { //you are recieveing the T frequencies
+ for (int k = 0; k < alignmentLength; k++) { F.t[k] += temp[k]; }
+ }else if (receiveTag == Ctag) { //you are recieveing the C frequencies
+ for (int k = 0; k < alignmentLength; k++) { F.c[k] += temp[k]; }
+ }else if (receiveTag == Gtag) { //you are recieveing the G frequencies
+ for (int k = 0; k < alignmentLength; k++) { F.g[k] += temp[k]; }
+ }else if (receiveTag == Gaptag) { //you are recieveing the gap frequencies
+ for (int k = 0; k < alignmentLength; k++) { F.gap[k] += temp[k]; }
+ }
+ }
+ }
+ }else{
+
+ //send my fequency counts
+ F.a.push_back(Atag);
+ int ierr = MPI_Send(&(F.a[0]), (alignmentLength+1), MPI_INT, 0, 2001, MPI_COMM_WORLD);
+ F.t.push_back(Ttag);
+ ierr = MPI_Send (&(F.t[0]), (alignmentLength+1), MPI_INT, 0, 2001, MPI_COMM_WORLD);
+ F.c.push_back(Ctag);
+ ierr = MPI_Send(&(F.c[0]), (alignmentLength+1), MPI_INT, 0, 2001, MPI_COMM_WORLD);
+ F.g.push_back(Gtag);
+ ierr = MPI_Send(&(F.g[0]), (alignmentLength+1), MPI_INT, 0, 2001, MPI_COMM_WORLD);
+ F.gap.push_back(Gaptag);
+ ierr = MPI_Send(&(F.gap[0]), (alignmentLength+1), MPI_INT, 0, 2001, MPI_COMM_WORLD);
+ }
+
}
- inFASTA.close();
- F.setNumSeqs(numSeqs);
+ MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case
- if(isTrue(vertical) == 1) { F.doVertical(); }
+ if (pid == 0) { //only one process should output the filter
+#endif
+
+ F.setNumSeqs(numSeqs);
+ if(m->isTrue(vertical) == 1) { F.doVertical(); }
if(soft != 0) { F.doSoft(); }
+ filterString = F.getFilter();
- filter = F.getFilter();
-
- ofstream outFilter;
- string filterFile = getRootName(fastafile) + "filter";
- openOutputFile(filterFile, outFilter);
- outFilter << filter << endl;
- outFilter.close();
+#ifdef USE_MPI
+ //send filter string to kids
+ //for(int i = 1; i < processors; i++) {
+ // MPI_Send(&filterString[0], alignmentLength, MPI_CHAR, i, 2001, MPI_COMM_WORLD);
+ //}
+ MPI_Bcast(&filterString[0], alignmentLength, MPI_CHAR, 0, MPI_COMM_WORLD);
+ }else{
+ //recieve filterString
+ char* tempBuf = new char[alignmentLength];
+ //MPI_Recv(&tempBuf[0], alignmentLength, MPI_CHAR, 0, 2001, MPI_COMM_WORLD, &status);
+ MPI_Bcast(tempBuf, alignmentLength, MPI_CHAR, 0, MPI_COMM_WORLD);
- ifstream inFasta2;
- openInputFile(fastafile, inFasta2);
- string filteredFasta = getRootName(fastafile) + "filter.fasta";
- ofstream outFASTA;
- openOutputFile(filteredFasta, outFASTA);
+ filterString = tempBuf;
+ if (filterString.length() > alignmentLength) { filterString = filterString.substr(0, alignmentLength); }
+ delete tempBuf;
+ }
+
+ MPI_Barrier(MPI_COMM_WORLD);
+#endif
+
+ return filterString;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "FilterSeqsCommand", "createFilter");
+ exit(1);
+ }
+}
+/**************************************************************************************/
+int FilterSeqsCommand::driverCreateFilter(Filters& F, string filename, linePair* filePos) {
+ try {
+
+ ifstream in;
+ m->openInputFile(filename, in);
+
+ in.seekg(filePos->start);
- numSeqs = 0;
- while(!inFasta2.eof()){
- Sequence seq(inFasta2);
- string align = seq.getAligned();
- string filterSeq = "";
+ bool done = false;
+ int count = 0;
- for(int j=0;j<alignmentLength;j++){
- if(filter[j] == '1'){
- filterSeq += align[j];
- }
+ while (!done) {
+
+ if (m->control_pressed) { in.close(); return 1; }
+
+ Sequence seq(in); m->gobble(in);
+ if (seq.getName() != "") {
+ if (seq.getAligned().length() != alignmentLength) { m->mothurOut("Sequences are not all the same length, please correct."); m->mothurOutEndLine(); m->control_pressed = true; }
+
+ if(trump != '*') { F.doTrump(seq); }
+ if(m->isTrue(vertical) || soft != 0) { F.getFreqs(seq); }
+ cout.flush();
+ count++;
}
-
- outFASTA << '>' << seq.getName() << endl << filterSeq << endl;
- numSeqs++;
- gobble(inFasta2);
+
+ #if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
+ unsigned long long pos = in.tellg();
+ if ((pos == -1) || (pos >= filePos->end)) { break; }
+ #else
+ if (in.eof()) { break; }
+ #endif
+
+ //report progress
+ if((count) % 100 == 0){ m->mothurOutJustToScreen(toString(count)+"\n"); }
}
- outFASTA.close();
- inFasta2.close();
+ //report progress
+ if((count) % 100 != 0){ m->mothurOutJustToScreen(toString(count)+"\n"); }
+ in.close();
+ return count;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "FilterSeqsCommand", "driverCreateFilter");
+ exit(1);
+ }
+}
+#ifdef USE_MPI
+/**************************************************************************************/
+int FilterSeqsCommand::MPICreateFilter(int start, int num, Filters& F, MPI_File& inMPI, vector<unsigned long long>& MPIPos) {
+ try {
- int filteredLength = 0;
- for(int i=0;i<alignmentLength;i++){
- if(filter[i] == '1'){ filteredLength++; }
+ MPI_Status status;
+ int pid;
+ MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
+
+ for(int i=0;i<num;i++){
+
+ if (m->control_pressed) { return 0; }
+
+ //read next sequence
+ int length = MPIPos[start+i+1] - MPIPos[start+i];
+
+ char* buf4 = new char[length];
+ MPI_File_read_at(inMPI, MPIPos[start+i], buf4, length, MPI_CHAR, &status);
+
+ string tempBuf = buf4;
+ if (tempBuf.length() > length) { tempBuf = tempBuf.substr(0, length); }
+ istringstream iss (tempBuf,istringstream::in);
+ delete buf4;
+
+ Sequence seq(iss);
+
+ if (seq.getAligned().length() != alignmentLength) { cout << "Alignment length is " << alignmentLength << " and sequence " << seq.getName() << " has length " << seq.getAligned().length() << ", please correct." << endl; exit(1); }
+
+ if(trump != '*'){ F.doTrump(seq); }
+ if(m->isTrue(vertical) || soft != 0){ F.getFreqs(seq); }
+ cout.flush();
+
+ //report progress
+ if((i+1) % 100 == 0){ cout << (i+1) << endl; }
}
- mothurOutEndLine();
- mothurOut("Length of filtered alignment: " + toString(filteredLength)); mothurOutEndLine();
- mothurOut("Number of columns removed: " + toString((alignmentLength-filteredLength))); mothurOutEndLine();
- mothurOut("Length of the original alignment: " + toString(alignmentLength)); mothurOutEndLine();
- mothurOut("Number of sequences used to construct filter: " + toString(numSeqs)); mothurOutEndLine();
+ //report progress
+ if((num) % 100 != 0){ cout << num << endl; }
return 0;
-
}
catch(exception& e) {
- errorOut(e, "FilterSeqsCommand", "execute");
+ m->errorOut(e, "FilterSeqsCommand", "MPICreateFilter");
exit(1);
}
}
+#endif
+/**************************************************************************************************/
+
+int FilterSeqsCommand::createProcessesCreateFilter(Filters& F, string filename) {
+ try {
+ int process = 1;
+ int num = 0;
+ processIDS.clear();
+
+#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
+
+ //loop through and create all the processes you want
+ while (process != processors) {
+ int pid = fork();
+
+ if (pid > 0) {
+ processIDS.push_back(pid); //create map from line number to pid so you can append files in correct order later
+ process++;
+ }else if (pid == 0){
+ //reset child's filter counts to 0;
+ F.a.clear(); F.a.resize(alignmentLength, 0);
+ F.t.clear(); F.t.resize(alignmentLength, 0);
+ F.g.clear(); F.g.resize(alignmentLength, 0);
+ F.c.clear(); F.c.resize(alignmentLength, 0);
+ F.gap.clear(); F.gap.resize(alignmentLength, 0);
+
+ num = driverCreateFilter(F, filename, lines[process]);
+
+ //write out filter counts to file
+ filename += toString(getpid()) + "filterValues.temp";
+ ofstream out;
+ m->openOutputFile(filename, out);
+
+ out << num << endl;
+ out << F.getFilter() << endl;
+ for (int k = 0; k < alignmentLength; k++) { out << F.a[k] << '\t'; } out << endl;
+ for (int k = 0; k < alignmentLength; k++) { out << F.t[k] << '\t'; } out << endl;
+ for (int k = 0; k < alignmentLength; k++) { out << F.g[k] << '\t'; } out << endl;
+ for (int k = 0; k < alignmentLength; k++) { out << F.c[k] << '\t'; } out << endl;
+ for (int k = 0; k < alignmentLength; k++) { out << F.gap[k] << '\t'; } out << endl;
+
+ //cout << F.getFilter() << endl;
+ out.close();
+
+ exit(0);
+ }else {
+ m->mothurOut("[ERROR]: unable to spawn the necessary processes."); m->mothurOutEndLine();
+ for (int i = 0; i < processIDS.size(); i++) { kill (processIDS[i], SIGINT); }
+ exit(0);
+ }
+ }
+
+ //parent do your part
+ num = driverCreateFilter(F, filename, lines[0]);
+
+ //force parent to wait until all the processes are done
+ for (int i=0;i<(processors-1);i++) {
+ int temp = processIDS[i];
+ wait(&temp);
+ }
+
+ //parent reads in and combines Filter info
+ for (int i = 0; i < processIDS.size(); i++) {
+ string tempFilename = filename + toString(processIDS[i]) + "filterValues.temp";
+ ifstream in;
+ m->openInputFile(tempFilename, in);
+
+ int temp, tempNum;
+ string tempFilterString;
+
+ in >> tempNum; m->gobble(in); num += tempNum;
+
+ in >> tempFilterString;
+ F.mergeFilter(tempFilterString);
+ for (int k = 0; k < alignmentLength; k++) { in >> temp; F.a[k] += temp; } m->gobble(in);
+ for (int k = 0; k < alignmentLength; k++) { in >> temp; F.t[k] += temp; } m->gobble(in);
+ for (int k = 0; k < alignmentLength; k++) { in >> temp; F.g[k] += temp; } m->gobble(in);
+ for (int k = 0; k < alignmentLength; k++) { in >> temp; F.c[k] += temp; } m->gobble(in);
+ for (int k = 0; k < alignmentLength; k++) { in >> temp; F.gap[k] += temp; } m->gobble(in);
+
+ in.close();
+ m->mothurRemove(tempFilename);
+ }
+
+
+#else
+
+ //////////////////////////////////////////////////////////////////////////////////////////////////////
+ //Windows version shared memory, so be careful when passing variables through the filterData struct.
+ //Above fork() will clone, so memory is separate, but that's not the case with windows,
+ //Taking advantage of shared memory to allow both threads to add info to F.
+ //////////////////////////////////////////////////////////////////////////////////////////////////////
+
+ vector<filterData*> pDataArray;
+ DWORD dwThreadIdArray[processors];
+ HANDLE hThreadArray[processors];
+
+ //Create processor worker threads.
+ for( int i=0; i<processors; i++ ){
+
+ filterData* tempFilter = new filterData(filename, m, lines[i]->start, lines[i]->end, alignmentLength, trump, vertical, soft, hard, i);
+ pDataArray.push_back(tempFilter);
+ processIDS.push_back(i);
+
+ hThreadArray[i] = CreateThread(NULL, 0, MyCreateFilterThreadFunction, pDataArray[i], 0, &dwThreadIdArray[i]);
+ }
+
+ //Wait until all threads have terminated.
+ WaitForMultipleObjects(processors, hThreadArray, TRUE, INFINITE);
+
+ //Close all thread handles and free memory allocations.
+ for(int i=0; i < pDataArray.size(); i++){
+ num += pDataArray[i]->count;
+ if (pDataArray[i]->count != pDataArray[i]->end) {
+ m->mothurOut("[ERROR]: process " + toString(i) + " only processed " + toString(pDataArray[i]->count) + " of " + toString(pDataArray[i]->end) + " sequences assigned to it, quitting. \n"); m->control_pressed = true;
+ }
+ F.mergeFilter(pDataArray[i]->F.getFilter());
+
+ for (int k = 0; k < alignmentLength; k++) { F.a[k] += pDataArray[i]->F.a[k]; }
+ for (int k = 0; k < alignmentLength; k++) { F.t[k] += pDataArray[i]->F.t[k]; }
+ for (int k = 0; k < alignmentLength; k++) { F.g[k] += pDataArray[i]->F.g[k]; }
+ for (int k = 0; k < alignmentLength; k++) { F.c[k] += pDataArray[i]->F.c[k]; }
+ for (int k = 0; k < alignmentLength; k++) { F.gap[k] += pDataArray[i]->F.gap[k]; }
+
+ CloseHandle(hThreadArray[i]);
+ delete pDataArray[i];
+ }
+
+#endif
+ return num;
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "FilterSeqsCommand", "createProcessesCreateFilter");
+ exit(1);
+ }
+}
/**************************************************************************************/
projects / mothur.git / blobdiff