#include "inputdata.h"
//**********************************************************************************************************************
-vector<string> ConsensusSeqsCommand::getValidParameters(){
+vector<string> ConsensusSeqsCommand::setParameters(){
try {
- string Array[] = {"fasta", "list", "name", "label","outputdir","inputdir"};
- vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
+ CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(pfasta);
+ CommandParameter pname("name", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(pname);
+ CommandParameter plist("list", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(plist);
+ CommandParameter plabel("label", "String", "", "", "", "", "",false,false); parameters.push_back(plabel);
+ CommandParameter pcutoff("cutoff", "Number", "", "100", "", "", "",false,false); parameters.push_back(pcutoff);
+ CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
+ CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir);
+
+ vector<string> myArray;
+ for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); }
return myArray;
}
catch(exception& e) {
- m->errorOut(e, "ConsensusSeqsCommand", "getValidParameters");
+ m->errorOut(e, "ConsensusSeqsCommand", "setParameters");
exit(1);
}
}
//**********************************************************************************************************************
-ConsensusSeqsCommand::ConsensusSeqsCommand(){
+string ConsensusSeqsCommand::getHelpString(){
try {
- abort = true;
- //initialize outputTypes
- vector<string> tempOutNames;
- outputTypes["fasta"] = tempOutNames;
- outputTypes["name"] = tempOutNames;
- outputTypes["summary"] = tempOutNames;
+ string helpString = "";
+ helpString += "The consensus.seqs command can be used in 2 ways: create a consensus sequence from a fastafile, or with a listfile create a consensus sequence for each otu. Sequences must be aligned.\n";
+ helpString += "The consensus.seqs command parameters are fasta, list, name, cutoff and label.\n";
+ helpString += "The fasta parameter allows you to enter the fasta file containing your sequences, and is required, unless you have a valid current fasta file. \n";
+ helpString += "The list parameter allows you to enter a your list file. \n";
+ helpString += "The name parameter allows you to enter a names file associated with the fasta file. \n";
+ helpString += "The label parameter allows you to select what distance levels you would like output files for, and are separated by dashes.\n";
+ helpString += "The cutoff parameter allows you set a percentage of sequences that support the base. For example: cutoff=97 would only return a sequence that only showed ambiguities for bases that were not supported by at least 97% of sequences.\n";
+ helpString += "The consensus.seqs command should be in the following format: \n";
+ helpString += "consensus.seqs(fasta=yourFastaFile, list=yourListFile) \n";
+ helpString += "Example: consensus.seqs(fasta=abrecovery.align, list=abrecovery.fn.list) \n";
+ helpString += "Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFastaFile).\n";
+ return helpString;
}
catch(exception& e) {
- m->errorOut(e, "ConsensusSeqsCommand", "ConsensusSeqsCommand");
- exit(1);
- }
-}
-//**********************************************************************************************************************
-vector<string> ConsensusSeqsCommand::getRequiredParameters(){
- try {
- string Array[] = {"fasta", "list"};
- vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
- return myArray;
- }
- catch(exception& e) {
- m->errorOut(e, "ConsensusSeqsCommand", "getRequiredParameters");
+ m->errorOut(e, "ConsensusSeqsCommand", "getHelpString");
exit(1);
}
}
+
//**********************************************************************************************************************
-vector<string> ConsensusSeqsCommand::getRequiredFiles(){
+ConsensusSeqsCommand::ConsensusSeqsCommand(){
try {
- vector<string> myArray;
- return myArray;
+ abort = true; calledHelp = true;
+ setParameters();
+ vector<string> tempOutNames;
+ outputTypes["fasta"] = tempOutNames;
+ outputTypes["name"] = tempOutNames;
+ outputTypes["summary"] = tempOutNames;
}
catch(exception& e) {
- m->errorOut(e, "DegapSeqsCommand", "getRequiredFiles");
+ m->errorOut(e, "ConsensusSeqsCommand", "ConsensusSeqsCommand");
exit(1);
}
}
//***************************************************************************************************************
ConsensusSeqsCommand::ConsensusSeqsCommand(string option) {
try {
- abort = false;
+ abort = false; calledHelp = false;
allLines = 1;
//allow user to run help
- if(option == "help") { help(); abort = true; }
+ if(option == "help") { help(); abort = true; calledHelp = true; }
+ else if(option == "citation") { citation(); abort = true; calledHelp = true;}
else {
- //valid paramters for this command
- string Array[] = {"fasta","list","name","label", "outputdir","inputdir"};
- vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
+
+ vector<string> myArray = setParameters();
OptionParser parser(option);
map<string,string> parameters = parser.getParameters();
//check for parameters
fastafile = validParameter.validFile(parameters, "fasta", true);
if (fastafile == "not open") { abort = true; }
- else if (fastafile == "not found") { fastafile = ""; m->mothurOut("fasta is a required parameter for the consensus.seqs command."); m->mothurOutEndLine(); abort = true; }
+ else if (fastafile == "not found") {
+ fastafile = m->getFastaFile();
+ if (fastafile != "") { m->mothurOut("Using " + fastafile + " as input file for the fasta parameter."); m->mothurOutEndLine(); }
+ else { m->mothurOut("You have no current fastafile and the fasta parameter is required."); m->mothurOutEndLine(); abort = true; }
+ }else { m->setFastaFile(fastafile); }
namefile = validParameter.validFile(parameters, "name", true);
if (namefile == "not open") { abort = true; }
- else if (namefile == "not found") { namefile = ""; }
+ else if (namefile == "not found") { namefile = ""; }
+ else { m->setNameFile(namefile); }
listfile = validParameter.validFile(parameters, "list", true);
if (listfile == "not open") { abort = true; }
- else if (listfile == "not found") { listfile = ""; m->mothurOut("list is a required parameter for the consensus.seqs command."); m->mothurOutEndLine(); abort = true; }
+ else if (listfile == "not found") { listfile = ""; }
+ else { m->setListFile(listfile); }
label = validParameter.validFile(parameters, "label", false);
if (label == "not found") { label = ""; }
else { allLines = 1; }
}
+ string temp = validParameter.validFile(parameters, "cutoff", false); if (temp == "not found") { temp = "100"; }
+ m->mothurConvert(temp, cutoff);
+
//if the user changes the output directory command factory will send this info to us in the output parameter
outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){ outputDir = m->hasPath(fastafile); }
exit(1);
}
}
-//**********************************************************************************************************************
-
-void ConsensusSeqsCommand::help(){
- try {
- m->mothurOut("The consensus.seqs command reads a fastafile and listfile and creates a consensus sequence for each otu. Sequences must be aligned.\n");
- m->mothurOut("The consensus.seqs command parameters are fasta, list, name and label.\n");
- m->mothurOut("The fasta parameter allows you to enter the fasta file containing your sequences, and is required. \n");
- m->mothurOut("The list parameter allows you to enter a your list file. \n");
- m->mothurOut("The name parameter allows you to enter a names file associated with the fasta file. \n");
- m->mothurOut("The label parameter allows you to select what distance levels you would like output files for, and are separated by dashes.\n");
- m->mothurOut("The consensus.seqs command should be in the following format: \n");
- m->mothurOut("consensus.seqs(fasta=yourFastaFile, list=yourListFile) \n");
- m->mothurOut("Example: consensus.seqs(fasta=abrecovery.align, list=abrecovery.fn.list) \n");
- m->mothurOut("Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFastaFile).\n\n");
- }
- catch(exception& e) {
- m->errorOut(e, "ConsensusSeqsCommand", "help");
- exit(1);
- }
-}
-
-//***************************************************************************************************************
-
-ConsensusSeqsCommand::~ConsensusSeqsCommand(){ /* do nothing */ }
-
//***************************************************************************************************************
int ConsensusSeqsCommand::execute(){
try{
- if (abort == true) { return 0; }
+ if (abort == true) { if (calledHelp) { return 0; } return 2; }
readFasta();
if (m->control_pressed) { return 0; }
- InputData* input = new InputData(listfile, "list");
- ListVector* list = input->getListVector();
-
- string lastLabel = list->getLabel();
- set<string> processedLabels;
- set<string> userLabels = labels;
-
- //as long as you are not at the end of the file or done wih the lines you want
- while((list != NULL) && ((allLines == 1) || (userLabels.size() != 0))) {
+
+ if (listfile == "") {
+
+ ofstream outSummary;
+ string outputSummaryFile = outputDir + m->getRootName(m->getSimpleName(fastafile)) + "cons.summary";
+ m->openOutputFile(outputSummaryFile, outSummary);
+ outSummary.setf(ios::fixed, ios::floatfield); outSummary.setf(ios::showpoint);
+ outputNames.push_back(outputSummaryFile); outputTypes["summary"].push_back(outputSummaryFile);
- if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { remove(outputNames[i].c_str()); } delete list; delete input; return 0; }
+ outSummary << "PositioninAlignment\tA\tT\tG\tC\tGap\tNumberofSeqs\tConsensusBase" << endl;
- if(allLines == 1 || labels.count(list->getLabel()) == 1){
+ ofstream outFasta;
+ string outputFastaFile = outputDir + m->getRootName(m->getSimpleName(fastafile)) + "cons.fasta";
+ m->openOutputFile(outputFastaFile, outFasta);
+ outputNames.push_back(outputFastaFile); outputTypes["fasta"].push_back(outputFastaFile);
+
+ vector<string> seqs;
+ int seqLength = 0;
+ for (map<string, string>::iterator it = nameMap.begin(); it != nameMap.end(); it++) {
- m->mothurOut(list->getLabel()); m->mothurOutEndLine();
+ if (m->control_pressed) { outSummary.close(); outFasta.close(); for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; }
- processList(list);
+ string seq = fastaMap[it->second];
+ seqs.push_back(seq);
- processedLabels.insert(list->getLabel());
- userLabels.erase(list->getLabel());
+ if (seqLength == 0) { seqLength = seq.length(); }
+ else if (seqLength != seq.length()) { m->mothurOut("[ERROR]: sequence are not the same length, please correct."); m->mothurOutEndLine(); m->control_pressed = true; }
+
}
- if ((m->anyLabelsToProcess(list->getLabel(), userLabels, "") == true) && (processedLabels.count(lastLabel) != 1)) {
- string saveLabel = list->getLabel();
+ vector< vector<float> > percentages; percentages.resize(5);
+ for (int j = 0; j < percentages.size(); j++) { percentages[j].resize(seqLength, 0.0); }
+
+ string consSeq = "";
+ //get counts
+ for (int j = 0; j < seqLength; j++) {
- delete list;
+ if (m->control_pressed) { outSummary.close(); outFasta.close(); for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; }
- list = input->getListVector(lastLabel);
- m->mothurOut(list->getLabel()); m->mothurOutEndLine();
+ vector<int> counts; counts.resize(5, 0); //A,T,G,C,Gap
+ int numDots = 0;
- processList(list);
+ for (int i = 0; i < seqs.size(); i++) {
+
+ if (seqs[i][j] == '.') { numDots++; }
+
+ char base = toupper(seqs[i][j]);
+ if (base == 'A') { counts[0]++; }
+ else if (base == 'T') { counts[1]++; }
+ else if (base == 'G') { counts[2]++; }
+ else if (base == 'C') { counts[3]++; }
+ else { counts[4]++; }
+ }
+
+ char conBase = '.';
+ if (numDots != seqs.size()) { conBase = getBase(counts, seqs.size()); }
- processedLabels.insert(list->getLabel());
- userLabels.erase(list->getLabel());
+ consSeq += conBase;
+
+ percentages[0][j] = counts[0] / (float) seqs.size();
+ percentages[1][j] = counts[1] / (float) seqs.size();
+ percentages[2][j] = counts[2] / (float) seqs.size();
+ percentages[3][j] = counts[3] / (float) seqs.size();
+ percentages[4][j] = counts[4] / (float) seqs.size();
- //restore real lastlabel to save below
- list->setLabel(saveLabel);
}
- lastLabel = list->getLabel();
+ for (int j = 0; j < seqLength; j++) {
+ outSummary << (j+1) << '\t' << percentages[0][j] << '\t'<< percentages[1][j] << '\t'<< percentages[2][j] << '\t' << percentages[3][j] << '\t' << percentages[4][j] << '\t' << seqs.size() << '\t' << consSeq[j] << endl;
+ }
- delete list; list = NULL;
+
+ outFasta << ">conseq" << endl << consSeq << endl;
+
+ outSummary.close(); outFasta.close();
- //get next line to process
- list = input->getListVector();
- }
-
-
- if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { remove(outputNames[i].c_str()); } if (list != NULL) { delete list; } delete input; return 0; }
- //output error messages about any remaining user labels
- set<string>::iterator it;
- bool needToRun = false;
- for (it = userLabels.begin(); it != userLabels.end(); it++) {
- m->mothurOut("Your file does not include the label " + *it);
- if (processedLabels.count(lastLabel) != 1) {
- m->mothurOut(". I will use " + lastLabel + "."); m->mothurOutEndLine();
- needToRun = true;
- }else {
- m->mothurOut(". Please refer to " + lastLabel + "."); m->mothurOutEndLine();
+ }else {
+
+
+ InputData* input = new InputData(listfile, "list");
+ ListVector* list = input->getListVector();
+
+ string lastLabel = list->getLabel();
+ set<string> processedLabels;
+ set<string> userLabels = labels;
+
+ //as long as you are not at the end of the file or done wih the lines you want
+ while((list != NULL) && ((allLines == 1) || (userLabels.size() != 0))) {
+
+ if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } delete list; delete input; return 0; }
+
+ if(allLines == 1 || labels.count(list->getLabel()) == 1){
+
+ m->mothurOut(list->getLabel()); m->mothurOutEndLine();
+
+ processList(list);
+
+ processedLabels.insert(list->getLabel());
+ userLabels.erase(list->getLabel());
+ }
+
+ if ((m->anyLabelsToProcess(list->getLabel(), userLabels, "") == true) && (processedLabels.count(lastLabel) != 1)) {
+ string saveLabel = list->getLabel();
+
+ delete list;
+
+ list = input->getListVector(lastLabel);
+ m->mothurOut(list->getLabel()); m->mothurOutEndLine();
+
+ processList(list);
+
+ processedLabels.insert(list->getLabel());
+ userLabels.erase(list->getLabel());
+
+ //restore real lastlabel to save below
+ list->setLabel(saveLabel);
+ }
+
+ lastLabel = list->getLabel();
+
+ delete list; list = NULL;
+
+ //get next line to process
+ list = input->getListVector();
}
- }
-
- //run last label if you need to
- if (needToRun == true) {
- if (list != NULL) { delete list; }
- list = input->getListVector(lastLabel);
- m->mothurOut(list->getLabel()); m->mothurOutEndLine();
+ if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } if (list != NULL) { delete list; } delete input; return 0; }
+
+ //output error messages about any remaining user labels
+ set<string>::iterator it;
+ bool needToRun = false;
+ for (it = userLabels.begin(); it != userLabels.end(); it++) {
+ m->mothurOut("Your file does not include the label " + *it);
+ if (processedLabels.count(lastLabel) != 1) {
+ m->mothurOut(". I will use " + lastLabel + "."); m->mothurOutEndLine();
+ needToRun = true;
+ }else {
+ m->mothurOut(". Please refer to " + lastLabel + "."); m->mothurOutEndLine();
+ }
+ }
- processList(list);
+ //run last label if you need to
+ if (needToRun == true) {
+ if (list != NULL) { delete list; }
+
+ list = input->getListVector(lastLabel);
+
+ m->mothurOut(list->getLabel()); m->mothurOutEndLine();
+
+ processList(list);
+
+ delete list; list = NULL;
+ }
- delete list; list = NULL;
+ if (list != NULL) { delete list; }
+ delete input;
}
- if (list != NULL) { delete list; }
- delete input;
-
m->mothurOutEndLine();
m->mothurOut("Output File Name: "); m->mothurOutEndLine();
for (int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i]); m->mothurOutEndLine(); }
m->openOutputFile(outputFastaFile, outFasta);
outputNames.push_back(outputFastaFile); outputTypes["fasta"].push_back(outputFastaFile);
+ outSummary << "OTU#\tPositioninAlignment\tA\tT\tG\tC\tGap\tNumberofSeqs\tConsensusBase" << endl;
+
for (int i = 0; i < list->getNumBins(); i++) {
if (m->control_pressed) { outSummary.close(); outName.close(); outFasta.close(); return 0; }
}
char conBase = '.';
- if (numDots != seqs.size()) { conBase = getBase(counts); }
+ if (numDots != seqs.size()) { conBase = getBase(counts, seqs.size()); }
consSeq += conBase;
}
- outSummary << ">seq" << (binNumber + 1) << endl;
- outSummary << "A" << '\t';
- for (int j = 0; j < seqLength; j++) { outSummary << percentages[0][j] << '\t'; }
- outSummary << endl;
- outSummary << "T" << '\t';
- for (int j = 0; j < seqLength; j++) { outSummary << percentages[1][j] << '\t'; }
- outSummary << endl;
- outSummary << "G" << '\t';
- for (int j = 0; j < seqLength; j++) { outSummary << percentages[2][j] << '\t'; }
- outSummary << endl;
- outSummary << "C" << '\t';
- for (int j = 0; j < seqLength; j++) { outSummary << percentages[3][j] << '\t'; }
- outSummary << endl;
- outSummary << "Gap" << '\t';
- for (int j = 0; j < seqLength; j++) { outSummary << percentages[4][j] << '\t'; }
- outSummary << endl;
-
+ for (int j = 0; j < seqLength; j++) {
+ outSummary << (binNumber + 1) << '\t' << (j+1) << '\t' << percentages[0][j] << '\t'<< percentages[1][j] << '\t'<< percentages[2][j] << '\t' << percentages[3][j] << '\t' << percentages[4][j] << '\t' << seqs.size() << '\t' << consSeq[j] << endl;
+ }
return consSeq;
}
//***************************************************************************************************************
-char ConsensusSeqsCommand::getBase(vector<int> counts){ //A,T,G,C,Gap
+char ConsensusSeqsCommand::getBase(vector<int> counts, int size){ //A,T,G,C,Gap
try{
/* A = adenine
* C = cytosine
char conBase = 'N';
+ //zero out counts that don't make the cutoff
+ float percentage = (100.0 - cutoff) / 100.0;
+ int zeroCutoff = percentage * size;
+
+ for (int i = 0; i < counts.size(); i++) {
+ if (counts[i] < zeroCutoff) { counts[i] = 0; }
+ }
+
//any
if ((counts[0] != 0) && (counts[1] != 0) && (counts[2] != 0) && (counts[3] != 0) && (counts[4] != 0)) { conBase = 'n'; }
//any no gap
else if ((counts[0] == 0) && (counts[1] == 0) && (counts[2] == 0) && (counts[3] != 0) && (counts[4] == 0)) { conBase = 'C'; }
//only gap
else if ((counts[0] == 0) && (counts[1] == 0) && (counts[2] == 0) && (counts[3] == 0) && (counts[4] != 0)) { conBase = '-'; }
+ //cutoff removed all counts
+ else if ((counts[0] == 0) && (counts[1] == 0) && (counts[2] == 0) && (counts[3] == 0) && (counts[4] == 0)) { conBase = 'N'; }
else{ m->mothurOut("[ERROR]: cannot find consensus base."); m->mothurOutEndLine(); }
return conBase;
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