]> git.donarmstrong.com Git - mothur.git/blobdiff - consensusseqscommand.cpp
changed random forest output filename
[mothur.git] / consensusseqscommand.cpp
index d6158ba3ed3bc147027d54098aa71398182dc2f6..3d671f98420e0fa7cf844aac647bd43feea959e8 100644 (file)
 //**********************************************************************************************************************
 vector<string> ConsensusSeqsCommand::setParameters(){  
        try {
-               CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "none", "none",false,true); parameters.push_back(pfasta);
-               CommandParameter pname("name", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(pname);
-               CommandParameter plist("list", "InputTypes", "", "", "none", "none", "none",false,false); parameters.push_back(plist);
-               CommandParameter plabel("label", "String", "", "", "", "", "",false,false); parameters.push_back(plabel);
-               CommandParameter pcutoff("cutoff", "Number", "", "100", "", "", "",false,false); parameters.push_back(pcutoff);
-               CommandParameter pinputdir("inputdir", "String", "", "", "", "", "",false,false); parameters.push_back(pinputdir);
-               CommandParameter poutputdir("outputdir", "String", "", "", "", "", "",false,false); parameters.push_back(poutputdir);
+               CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "none", "none","fasta-name",false,true,true); parameters.push_back(pfasta);
+               CommandParameter pname("name", "InputTypes", "", "", "namecount", "none", "none","name",false,false,true); parameters.push_back(pname);
+        CommandParameter pcount("count", "InputTypes", "", "", "namecount", "none", "none","count",false,false,true); parameters.push_back(pcount);
+               CommandParameter plist("list", "InputTypes", "", "", "none", "none", "none","fasta-name",false,false,true); parameters.push_back(plist);
+               CommandParameter plabel("label", "String", "", "", "", "", "","",false,false); parameters.push_back(plabel);
+               CommandParameter pcutoff("cutoff", "Number", "", "100", "", "", "","",false,false); parameters.push_back(pcutoff);
+               CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
+               CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
                
                vector<string> myArray;
                for (int i = 0; i < parameters.size(); i++) {   myArray.push_back(parameters[i].name);          }
@@ -36,7 +37,7 @@ string ConsensusSeqsCommand::getHelpString(){
        try {
                string helpString = "";
                helpString += "The consensus.seqs command can be used in 2 ways: create a consensus sequence from a fastafile, or with a listfile create a consensus sequence for each otu. Sequences must be aligned.\n";
-               helpString += "The consensus.seqs command parameters are fasta, list, name, cutoff and label.\n";
+               helpString += "The consensus.seqs command parameters are fasta, list, name, count, cutoff and label.\n";
                helpString += "The fasta parameter allows you to enter the fasta file containing your sequences, and is required, unless you have a valid current fasta file. \n";
                helpString += "The list parameter allows you to enter a your list file. \n";
                helpString += "The name parameter allows you to enter a names file associated with the fasta file. \n";
@@ -54,28 +55,23 @@ string ConsensusSeqsCommand::getHelpString(){
        }
 }
 //**********************************************************************************************************************
-string ConsensusSeqsCommand::getOutputFileNameTag(string type, string inputName=""){   
-       try {
-        string outputFileName = "";
-               map<string, vector<string> >::iterator it;
+string ConsensusSeqsCommand::getOutputPattern(string type) {
+    try {
+        string pattern = "";
         
-        //is this a type this command creates
-        it = outputTypes.find(type);
-        if (it == outputTypes.end()) {  m->mothurOut("[ERROR]: this command doesn't create a " + type + " output file.\n"); }
-        else {
-            if (type == "fasta") {  outputFileName =  "cons.fasta"; }
-            else if (type == "name") {  outputFileName =  "cons.names"; }
-            else if (type == "summary") {  outputFileName =  "cons.summary"; }
-            else { m->mothurOut("[ERROR]: No definition for type " + type + " output file tag.\n"); m->control_pressed = true;  }
-        }
-        return outputFileName;
-       }
-       catch(exception& e) {
-               m->errorOut(e, "ConsensusSeqsCommand", "getOutputFileNameTag");
-               exit(1);
-       }
+        if (type == "fasta") {  pattern = "[filename],cons.fasta-[filename],[tag],cons.fasta"; } 
+        else if (type == "name") {  pattern = "[filename],cons.names-[filename],[tag],cons.names"; } 
+        else if (type == "count") {  pattern = "[filename],cons.count_table-[filename],[tag],cons.count_table"; }
+        else if (type == "summary") {  pattern = "[filename],cons.summary-[filename],[tag],cons.summary"; }
+        else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->control_pressed = true;  }
+        
+        return pattern;
+    }
+    catch(exception& e) {
+        m->errorOut(e, "ConsensusSeqsCommand", "getOutputPattern");
+        exit(1);
+    }
 }
-
 //**********************************************************************************************************************
 ConsensusSeqsCommand::ConsensusSeqsCommand(){  
        try {
@@ -84,6 +80,7 @@ ConsensusSeqsCommand::ConsensusSeqsCommand(){
                vector<string> tempOutNames;
                outputTypes["fasta"] = tempOutNames;
                outputTypes["name"] = tempOutNames;
+        outputTypes["count"] = tempOutNames;
                outputTypes["summary"] = tempOutNames;
        }
        catch(exception& e) {
@@ -120,6 +117,7 @@ ConsensusSeqsCommand::ConsensusSeqsCommand(string option)  {
                        vector<string> tempOutNames;
                        outputTypes["fasta"] = tempOutNames;
                        outputTypes["name"] = tempOutNames;
+            outputTypes["count"] = tempOutNames;
                        outputTypes["summary"] = tempOutNames;
                        
                                                
@@ -151,6 +149,14 @@ ConsensusSeqsCommand::ConsensusSeqsCommand(string option)  {
                                        //if the user has not given a path then, add inputdir. else leave path alone.
                                        if (path == "") {       parameters["list"] = inputDir + it->second;             }
                                }
+                
+                it = parameters.find("count");
+                               //user has given a template file
+                               if(it != parameters.end()){ 
+                                       path = m->hasPath(it->second);
+                                       //if the user has not given a path then, add inputdir. else leave path alone.
+                                       if (path == "") {       parameters["count"] = inputDir + it->second;            }
+                               }
                        }
                        
                        
@@ -168,6 +174,13 @@ ConsensusSeqsCommand::ConsensusSeqsCommand(string option)  {
                        else if (namefile == "not found") { namefile = ""; }
                        else { m->setNameFile(namefile); }
                        
+            countfile = validParameter.validFile(parameters, "count", true);
+                       if (countfile == "not open") { abort = true; countfile = ""; }  
+                       else if (countfile == "not found") { countfile = ""; }
+                       else { m->setCountTableFile(countfile); }
+                       
+            if ((countfile != "") && (namefile != "")) { m->mothurOut("You must enter ONLY ONE of the following: count or name."); m->mothurOutEndLine(); abort = true; }
+            
                        listfile = validParameter.validFile(parameters, "list", true);
                        if (listfile == "not open") { abort = true; }
                        else if (listfile == "not found") { listfile = "";  }   
@@ -186,10 +199,12 @@ ConsensusSeqsCommand::ConsensusSeqsCommand(string option)  {
                        //if the user changes the output directory command factory will send this info to us in the output parameter 
                        outputDir = validParameter.validFile(parameters, "outputdir", false);           if (outputDir == "not found"){  outputDir = m->hasPath(fastafile);      }
                        
-                       if (namefile == ""){
-                               vector<string> files; files.push_back(fastafile); 
-                               parser.getNameFile(files);
-                       }
+            if (countfile == "") {
+                if (namefile == ""){
+                    vector<string> files; files.push_back(fastafile); 
+                    parser.getNameFile(files);
+                }
+            }
                }
        }
        catch(exception& e) {
@@ -204,11 +219,14 @@ int ConsensusSeqsCommand::execute(){
                
                if (abort == true) { if (calledHelp) { return 0; }  return 2;   }
                
+        int start = time(NULL);
+        
                readFasta();
                
                if (m->control_pressed) { return 0; }
                
                if (namefile != "") { readNames(); }
+        if (countfile != "") { ct.readTable(countfile, true);  }
                
                if (m->control_pressed) { return 0; }
                
@@ -216,7 +234,9 @@ int ConsensusSeqsCommand::execute(){
                if (listfile == "") {
                        
                        ofstream outSummary;
-                       string outputSummaryFile = outputDir + m->getRootName(m->getSimpleName(fastafile)) + getOutputFileNameTag("summary");
+            map<string, string> variables; 
+            variables["[filename]"] = outputDir + m->getRootName(m->getSimpleName(fastafile));
+                       string outputSummaryFile = getOutputFileName("summary", variables);
                        m->openOutputFile(outputSummaryFile, outSummary);
                        outSummary.setf(ios::fixed, ios::floatfield); outSummary.setf(ios::showpoint);
                        outputNames.push_back(outputSummaryFile); outputTypes["summary"].push_back(outputSummaryFile);
@@ -224,28 +244,15 @@ int ConsensusSeqsCommand::execute(){
                        outSummary << "PositioninAlignment\tA\tT\tG\tC\tGap\tNumberofSeqs\tConsensusBase" << endl;
                        
                        ofstream outFasta;
-                       string outputFastaFile = outputDir + m->getRootName(m->getSimpleName(fastafile)) + getOutputFileNameTag("fasta");
+                       string outputFastaFile = getOutputFileName("fasta", variables);
                        m->openOutputFile(outputFastaFile, outFasta);
                        outputNames.push_back(outputFastaFile); outputTypes["fasta"].push_back(outputFastaFile);
-                       
-                       vector<string> seqs;
-                       int seqLength = 0;
-                       for (map<string, string>::iterator it = nameMap.begin(); it != nameMap.end(); it++) {
-                               
-                               if (m->control_pressed) { outSummary.close(); outFasta.close(); for (int i = 0; i < outputNames.size(); i++) {  m->mothurRemove(outputNames[i]); } return 0; }
-                               
-                               string seq = fastaMap[it->second];
-                               seqs.push_back(seq);
-                               
-                               if (seqLength == 0) { seqLength = seq.length(); }
-                               else if (seqLength != seq.length()) { m->mothurOut("[ERROR]: sequence are not the same length, please correct."); m->mothurOutEndLine(); m->control_pressed = true; }
-
-                       }
-                       
+        
                        vector< vector<float> > percentages; percentages.resize(5);
                        for (int j = 0; j < percentages.size(); j++) { percentages[j].resize(seqLength, 0.0); }
                        
                        string consSeq = "";
+            int thisCount;
                        //get counts
                        for (int j = 0; j < seqLength; j++) {
                                
@@ -253,45 +260,59 @@ int ConsensusSeqsCommand::execute(){
                                
                                vector<int> counts; counts.resize(5, 0); //A,T,G,C,Gap
                                int numDots = 0;
-                               
-                               for (int i = 0; i < seqs.size(); i++) {
+                               thisCount = 0;
+                               for (map<string, string>::iterator it = fastaMap.begin(); it != fastaMap.end(); it++) {
                                        
-                                       if (seqs[i][j] == '.') { numDots++; }
-                                       
-                                       char base = toupper(seqs[i][j]);
-                                       if (base == 'A') { counts[0]++; }
-                                       else if (base == 'T') { counts[1]++; }
-                                       else if (base == 'G') { counts[2]++; }
-                                       else if (base == 'C') { counts[3]++; }
-                                       else { counts[4]++; }
+                    string thisSeq = it->second;
+                    int size = 0;
+                    
+                    if (countfile != "") { size = ct.getNumSeqs(it->first); }
+                    else {
+                        map<string, int>::iterator itCount = nameFileMap.find(it->first);
+                        if (itCount != nameFileMap.end()) {
+                            size = itCount->second;
+                        }else { m->mothurOut("[ERROR]: file mismatch, aborting.\n"); m->control_pressed = true; break; }
+                    }
+                    
+                    for (int k = 0; k < size; k++) {
+                        if (thisSeq[j] == '.') { numDots++; }
+                        
+                        char base = toupper(thisSeq[j]);
+                        if (base == 'A') { counts[0]++; }
+                        else if (base == 'T') { counts[1]++; }
+                        else if (base == 'G') { counts[2]++; }
+                        else if (base == 'C') { counts[3]++; }
+                        else { counts[4]++; }
+                        thisCount++;
+                    }
                                }
                                
                                char conBase = '.';
-                               if (numDots != seqs.size()) { conBase = getBase(counts, seqs.size()); }
+                               if (numDots != thisCount) { conBase = getBase(counts, thisCount); }
                                
                                consSeq += conBase;
                                
-                               percentages[0][j] = counts[0] / (float) seqs.size();
-                               percentages[1][j] = counts[1] / (float) seqs.size();
-                               percentages[2][j] = counts[2] / (float) seqs.size();
-                               percentages[3][j] = counts[3] / (float) seqs.size();
-                               percentages[4][j] = counts[4] / (float) seqs.size();
-                               
+                               percentages[0][j] = counts[0] / (float) thisCount;
+                               percentages[1][j] = counts[1] / (float) thisCount;
+                               percentages[2][j] = counts[2] / (float) thisCount;
+                               percentages[3][j] = counts[3] / (float) thisCount;
+                               percentages[4][j] = counts[4] / (float) thisCount;
                        }
                        
                        for (int j = 0; j < seqLength; j++) { 
-                               outSummary << (j+1) << '\t' << percentages[0][j] << '\t'<< percentages[1][j] << '\t'<< percentages[2][j] << '\t' << percentages[3][j] << '\t' << percentages[4][j] << '\t' << seqs.size() << '\t' << consSeq[j] << endl;
+                               outSummary << (j+1) << '\t' << percentages[0][j] << '\t'<< percentages[1][j] << '\t'<< percentages[2][j] << '\t' << percentages[3][j] << '\t' << percentages[4][j] << '\t' << thisCount << '\t' << consSeq[j] << endl;
                        }
                        
                                
                        outFasta << ">conseq" << endl << consSeq << endl;
                        
                        outSummary.close(); outFasta.close();
-                       
+            
+                       if (m->control_pressed) {  for (int i = 0; i < outputNames.size(); i++) {       m->mothurRemove(outputNames[i]); } return 0; }
                
                }else {
                        
-                                               
+            
                        InputData* input = new InputData(listfile, "list");
                        ListVector* list = input->getListVector();
                        
@@ -372,8 +393,10 @@ int ConsensusSeqsCommand::execute(){
                        delete input;
                }
                
+        m->mothurOut("It took " + toString(time(NULL) - start) + " secs to find the consensus sequences.");
+        
                m->mothurOutEndLine();
-               m->mothurOut("Output File Name: "); m->mothurOutEndLine();
+               m->mothurOut("Output File Names: "); m->mothurOutEndLine();
                for (int i = 0; i < outputNames.size(); i++) {  m->mothurOut(outputNames[i]); m->mothurOutEndLine();    }       
                m->mothurOutEndLine();
                
@@ -392,34 +415,44 @@ int ConsensusSeqsCommand::processList(ListVector*& list){
        try{
                
                ofstream outSummary;
-               string outputSummaryFile = outputDir + m->getRootName(m->getSimpleName(fastafile)) + list->getLabel() + getOutputFileNameTag("summary");
+        map<string, string> variables; 
+        variables["[filename]"] = outputDir + m->getRootName(m->getSimpleName(fastafile));
+        variables["[tag]"] = list->getLabel();
+               string outputSummaryFile = getOutputFileName("summary", variables);
                m->openOutputFile(outputSummaryFile, outSummary);
                outSummary.setf(ios::fixed, ios::floatfield); outSummary.setf(ios::showpoint);
                outputNames.push_back(outputSummaryFile); outputTypes["summary"].push_back(outputSummaryFile);
                
                ofstream outName;
-               string outputNameFile = outputDir + m->getRootName(m->getSimpleName(fastafile)) + list->getLabel() + getOutputFileNameTag("name");
+               string outputNameFile = getOutputFileName("name",variables);
                m->openOutputFile(outputNameFile, outName);
                outputNames.push_back(outputNameFile); outputTypes["name"].push_back(outputNameFile);
                
                ofstream outFasta;
-               string outputFastaFile = outputDir + m->getRootName(m->getSimpleName(fastafile)) + list->getLabel() + getOutputFileNameTag("fasta");
+               string outputFastaFile = getOutputFileName("fasta",variables);
                m->openOutputFile(outputFastaFile, outFasta);
                outputNames.push_back(outputFastaFile); outputTypes["fasta"].push_back(outputFastaFile);
                
                outSummary << "OTU#\tPositioninAlignment\tA\tT\tG\tC\tGap\tNumberofSeqs\tConsensusBase" << endl;
                
+        string snumBins = toString(list->getNumBins());
                for (int i = 0; i < list->getNumBins(); i++) {
                        
                        if (m->control_pressed) { outSummary.close(); outName.close(); outFasta.close(); return 0; }
                        
                        string bin = list->get(i);
-                       
-                       string newName = "";
-                       string consSeq = getConsSeq(bin, outSummary, newName, i);
-                       
-                       outFasta << ">seq" << (i+1) << endl << consSeq << endl;
-                       outName << "seq" << (i+1) << '\t' << "seq" << (i+1) << "," << newName << endl;
+                       string consSeq = getConsSeq(bin, outSummary, i);
+            
+            string seqName = "Otu";
+            string sbinNumber = toString(i+1);
+            if (sbinNumber.length() < snumBins.length()) {
+                int diff = snumBins.length() - sbinNumber.length();
+                for (int h = 0; h < diff; h++) { seqName += "0"; }
+            }
+            seqName += sbinNumber;
+                       
+                       outFasta << ">" << seqName << endl << consSeq << endl;
+                       outName << seqName << '\t' << seqName << "," << bin << endl;
                }
                
                outSummary.close(); outName.close(); outFasta.close();
@@ -434,96 +467,127 @@ int ConsensusSeqsCommand::processList(ListVector*& list){
 }
 
 //***************************************************************************************************************
-//made this smart enough to owrk with unique or non unique list file
-string ConsensusSeqsCommand::getConsSeq(string bin, ofstream& outSummary, string& name, int binNumber){
+string ConsensusSeqsCommand::getConsSeq(string bin, ofstream& outSummary, int binNumber){
        try{
                
                string consSeq = "";
                bool error = false;
-               
-               //the whole bin is the second column if no names file, otherwise build it
-               name = bin;
-               if (namefile != "") { name = ""; }
-               
+        int totalSize=0;
+                               
                vector<string> binNames;
                m->splitAtComma(bin, binNames);
-               
-               //get sequence strings for each name in the bin
-               vector<string> seqs;
-               
-               set<string> addedAlready;
-               int seqLength = 0;
-               for (int i = 0; i < binNames.size(); i++) {
-                       
-                       map<string, string>::iterator it;
-                       
-                       it = nameMap.find(binNames[i]);
-                       if (it == nameMap.end()) { 
-                               if (namefile == "") { m->mothurOut("[ERROR]: " + binNames[i] + " is not in your fasta file, please correct."); m->mothurOutEndLine(); error = true; }
-                               else { m->mothurOut("[ERROR]: " + binNames[i] + " is not in your fasta or name file, please correct."); m->mothurOutEndLine(); error = true; }
-                               break;
-                       }else {
-                               
-                               //add sequence string to seqs vector to process below
-                               string seq = fastaMap[it->second];
-                               seqs.push_back(seq);
-                               
-                               if (seqLength == 0) { seqLength = seq.length(); }
-                               else if (seqLength != seq.length()) { m->mothurOut("[ERROR]: sequence are not the same length, please correct."); m->mothurOutEndLine(); error = true; break; }
-                               
-                               if (namefile != "") { 
-                                       //did we add this line from name file already?
-                                       if (addedAlready.count(it->second) == 0) {
-                                               name += "," + nameFileMap[it->second];
-                                               addedAlready.insert(it->second);
-                                       }
-                               }
-                               
-                       }
-               }
-               
-               if (error) { m->control_pressed = true; return consSeq; }
-               
-               if (namefile != "") { name = name.substr(1); }
-               
-               vector< vector<float> > percentages; percentages.resize(5);
+        
+        vector< vector<float> > percentages; percentages.resize(5);
                for (int j = 0; j < percentages.size(); j++) { percentages[j].resize(seqLength, 0.0); }
-               
-               //get counts
-               for (int j = 0; j < seqLength; j++) {
-                       
-                       if (m->control_pressed) { return consSeq; }
-                       
-                       vector<int> counts; counts.resize(5, 0); //A,T,G,C,Gap
-                       int numDots = 0;
-                       
-                       for (int i = 0; i < seqs.size(); i++) {
-                               
-                               if (seqs[i][j] == '.') { numDots++; }
-                               
-                               char base = toupper(seqs[i][j]);
-                               if (base == 'A') { counts[0]++; }
-                               else if (base == 'T') { counts[1]++; }
-                               else if (base == 'G') { counts[2]++; }
-                               else if (base == 'C') { counts[3]++; }
-                               else { counts[4]++; }
-                       }
-                       
-                       char conBase = '.';
-                       if (numDots != seqs.size()) { conBase = getBase(counts, seqs.size()); }
-                       
-                       consSeq += conBase;
-                       
-                       percentages[0][j] = counts[0] / (float) seqs.size();
-                       percentages[1][j] = counts[1] / (float) seqs.size();
-                       percentages[2][j] = counts[2] / (float) seqs.size();
-                       percentages[3][j] = counts[3] / (float) seqs.size();
-                       percentages[4][j] = counts[4] / (float) seqs.size();
-                       
+
+        if (countfile != "") {
+            //get counts
+            for (int j = 0; j < seqLength; j++) {
+                
+                if (m->control_pressed) { return consSeq; }
+                
+                vector<int> counts; counts.resize(5, 0); //A,T,G,C,Gap
+                int numDots = 0;
+                totalSize = 0;
+                 for (int i = 0; i < binNames.size(); i++) {
+                     if (m->control_pressed) { return consSeq; }
+                     
+                     string thisSeq = "";
+                     map<string, string>::iterator itFasta = fastaMap.find(binNames[i]);
+                     if (itFasta != fastaMap.end()) {
+                         thisSeq = itFasta->second;
+                     }else { m->mothurOut("[ERROR]: " + binNames[i] + " is not in your fasta file, please correct."); m->mothurOutEndLine(); m->control_pressed = true; }
+                     
+                     int size = ct.getNumSeqs(binNames[i]);
+                     if (size != 0) {
+                         for (int k = 0; k < size; k++) {
+                             if (thisSeq[j] == '.') { numDots++; }
+                             
+                             char base = toupper(thisSeq[j]);
+                             if (base == 'A') { counts[0]++; }
+                             else if (base == 'T') { counts[1]++; }
+                             else if (base == 'G') { counts[2]++; }
+                             else if (base == 'C') { counts[3]++; }
+                             else { counts[4]++; }
+                             totalSize++;
+                         }
+                     }else { m->mothurOut("[ERROR]: " + binNames[i] + " is not in your count file, please correct."); m->mothurOutEndLine(); m->control_pressed = true; }
+                 }
+                char conBase = '.';
+                if (numDots != totalSize) { conBase = getBase(counts, totalSize); }
+                
+                consSeq += conBase;
+                
+                percentages[0][j] = counts[0] / (float) totalSize;
+                percentages[1][j] = counts[1] / (float) totalSize;
+                percentages[2][j] = counts[2] / (float) totalSize;
+                percentages[3][j] = counts[3] / (float) totalSize;
+                percentages[4][j] = counts[4] / (float) totalSize;
+            }
+
+        }else {
+               
+            //get sequence strings for each name in the bin
+            vector<string> seqs;
+            for (int i = 0; i < binNames.size(); i++) {
+                
+                map<string, string>::iterator it;
+                it = nameMap.find(binNames[i]);
+                if (it == nameMap.end()) { 
+                    if (namefile == "") { m->mothurOut("[ERROR]: " + binNames[i] + " is not in your fasta file, please correct."); m->mothurOutEndLine(); error = true; }
+                    else { m->mothurOut("[ERROR]: " + binNames[i] + " is not in your fasta or name file, please correct."); m->mothurOutEndLine(); error = true; }
+                    break;
+                }else {
+                    //add sequence string to seqs vector to process below
+                    map<string, string>::iterator itFasta = fastaMap.find(it->second);
+                    
+                    if (itFasta != fastaMap.end()) {
+                        string seq = itFasta->second;
+                        seqs.push_back(seq);
+                    }else { m->mothurOut("[ERROR]: file mismatch, aborting. \n"); }
+                }
+            }
+            
+            if (error) { m->control_pressed = true; return consSeq; }
+            totalSize = seqs.size();
+            //get counts
+            for (int j = 0; j < seqLength; j++) {
+                
+                if (m->control_pressed) { return consSeq; }
+                
+                vector<int> counts; counts.resize(5, 0); //A,T,G,C,Gap
+                int numDots = 0;
+                
+                for (int i = 0; i < seqs.size(); i++) {
+                    
+                    if (seqs[i][j] == '.') { numDots++; }
+                    
+                    char base = toupper(seqs[i][j]);
+                    if (base == 'A') { counts[0]++; }
+                    else if (base == 'T') { counts[1]++; }
+                    else if (base == 'G') { counts[2]++; }
+                    else if (base == 'C') { counts[3]++; }
+                    else { counts[4]++; }
+                }
+                
+                char conBase = '.';
+                if (numDots != seqs.size()) { conBase = getBase(counts, seqs.size()); }
+                
+                consSeq += conBase;
+                
+                percentages[0][j] = counts[0] / (float) seqs.size();
+                percentages[1][j] = counts[1] / (float) seqs.size();
+                percentages[2][j] = counts[2] / (float) seqs.size();
+                percentages[3][j] = counts[3] / (float) seqs.size();
+                percentages[4][j] = counts[4] / (float) seqs.size();
+                
+            }
                }
-               
+        
+        
+        
                for (int j = 0; j < seqLength; j++) { 
-                       outSummary << (binNumber + 1) << '\t' << (j+1) << '\t' << percentages[0][j] << '\t'<< percentages[1][j] << '\t'<< percentages[2][j] << '\t' << percentages[3][j] << '\t' << percentages[4][j] << '\t' << seqs.size() << '\t' << consSeq[j] << endl;
+                       outSummary << (binNumber + 1) << '\t' << (j+1) << '\t' << percentages[0][j] << '\t'<< percentages[1][j] << '\t'<< percentages[2][j] << '\t' << percentages[3][j] << '\t' << percentages[4][j] << '\t' << totalSize << '\t' << consSeq[j] << endl;
                }
                
                return consSeq;
@@ -558,10 +622,10 @@ char ConsensusSeqsCommand::getBase(vector<int> counts, int size){  //A,T,G,C,Gap
                
                //zero out counts that don't make the cutoff
                float percentage = (100.0 - cutoff) / 100.0;
-               int zeroCutoff = percentage * size;
-               
+        
                for (int i = 0; i < counts.size(); i++) {
-                       if (counts[i] < zeroCutoff) { counts[i] = 0; }
+            float countPercentage = counts[i] / (float) size;
+                       if (countPercentage < percentage) { counts[i] = 0; }
                }
                
                //any
@@ -646,7 +710,8 @@ int ConsensusSeqsCommand::readFasta(){
                
                ifstream in;
                m->openInputFile(fastafile, in);
-               
+               seqLength = 0;
+        
                while (!in.eof()) {
                        
                        if (m->control_pressed) { break; }
@@ -657,7 +722,10 @@ int ConsensusSeqsCommand::readFasta(){
                        if (name != "") {
                                fastaMap[name] = seq.getAligned();
                                nameMap[name] = name; //set nameMap incase no names file
-                               nameFileMap[name] = name;
+                               nameFileMap[name] = 1;
+                
+                if (seqLength == 0) { seqLength = seq.getAligned().length(); }
+                               else if (seqLength != seq.getAligned().length()) { m->mothurOut("[ERROR]: sequence are not the same length, please correct."); m->mothurOutEndLine(); m->control_pressed = true; break; }
                        }
                }
                
@@ -688,7 +756,7 @@ int ConsensusSeqsCommand::readNames(){
              
              it = nameMap.find(thisname);
                         if (it != nameMap.end()) { //then this sequence was in the fastafile
-                                nameFileMap[thisname] = repnames;      //for later when outputting the new namesFile if the list file is unique
+                                nameFileMap[thisname] = m->getNumNames(repnames);      //for later when outputting the new namesFile if the list file is unique
                  
                                 vector<string> splitRepNames;
                                 m->splitAtComma(repnames, splitRepNames);