#include "readmatrix.hpp"
#include "inputdata.h"
+
+//**********************************************************************************************************************
+vector<string> ClusterSplitCommand::getValidParameters(){
+ try {
+ string AlignArray[] = {"fasta","phylip","column","name","cutoff","precision","method","splitmethod","taxonomy","taxlevel","large","showabund","timing","hard","processors","outputdir","inputdir"};
+ vector<string> myArray (AlignArray, AlignArray+(sizeof(AlignArray)/sizeof(string)));
+ return myArray;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClusterSplitCommand", "getValidParameters");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+ClusterSplitCommand::ClusterSplitCommand(){
+ try {
+ abort = true;
+ //initialize outputTypes
+ vector<string> tempOutNames;
+ outputTypes["list"] = tempOutNames;
+ outputTypes["rabund"] = tempOutNames;
+ outputTypes["sabund"] = tempOutNames;
+ outputTypes["column"] = tempOutNames;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClusterSplitCommand", "ClusterSplitCommand");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+vector<string> ClusterSplitCommand::getRequiredParameters(){
+ try {
+ string Array[] = {"fasta","phylip","column","or"};
+ vector<string> myArray (Array, Array+(sizeof(Array)/sizeof(string)));
+ return myArray;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClusterSplitCommand", "getRequiredParameters");
+ exit(1);
+ }
+}
+//**********************************************************************************************************************
+vector<string> ClusterSplitCommand::getRequiredFiles(){
+ try {
+ vector<string> myArray;
+ return myArray;
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClusterSplitCommand", "getRequiredFiles");
+ exit(1);
+ }
+}
//**********************************************************************************************************************
//This function checks to make sure the cluster command has no errors and then clusters based on the method chosen.
ClusterSplitCommand::ClusterSplitCommand(string option) {
OptionParser parser(option);
map<string,string> parameters = parser.getParameters();
- ValidParameters validParameter;
+ ValidParameters validParameter("cluster.split");
//check to make sure all parameters are valid for command
map<string,string>::iterator it;
}
}
+ //initialize outputTypes
+ vector<string> tempOutNames;
+ outputTypes["list"] = tempOutNames;
+ outputTypes["rabund"] = tempOutNames;
+ outputTypes["sabund"] = tempOutNames;
+ outputTypes["column"] = tempOutNames;
+
globaldata->newRead();
//if the user changes the output directory command factory will send this info to us in the output parameter
it = parameters.find("phylip");
//user has given a template file
if(it != parameters.end()){
- path = hasPath(it->second);
+ path = m->hasPath(it->second);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["phylip"] = inputDir + it->second; }
}
it = parameters.find("column");
//user has given a template file
if(it != parameters.end()){
- path = hasPath(it->second);
+ path = m->hasPath(it->second);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["column"] = inputDir + it->second; }
}
it = parameters.find("name");
//user has given a template file
if(it != parameters.end()){
- path = hasPath(it->second);
+ path = m->hasPath(it->second);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["name"] = inputDir + it->second; }
}
it = parameters.find("taxonomy");
//user has given a template file
if(it != parameters.end()){
- path = hasPath(it->second);
+ path = m->hasPath(it->second);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["taxonomy"] = inputDir + it->second; }
}
it = parameters.find("fasta");
//user has given a template file
if(it != parameters.end()){
- path = hasPath(it->second);
+ path = m->hasPath(it->second);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["fasta"] = inputDir + it->second; }
}
convert(temp, precision);
temp = validParameter.validFile(parameters, "hard", false); if (temp == "not found") { temp = "F"; }
- hard = isTrue(temp);
+ hard = m->isTrue(temp);
temp = validParameter.validFile(parameters, "large", false); if (temp == "not found") { temp = "F"; }
- large = isTrue(temp);
+ large = m->isTrue(temp);
temp = validParameter.validFile(parameters, "processors", false); if (temp == "not found"){ temp = "1"; }
convert(temp, processors);
m->mothurOut("The cluster.split command can split your files in 3 ways. Splitting by distance file, by classification, or by classification also using a fasta file. \n");
m->mothurOut("For the distance file method, you need only provide your distance file and mothur will split the file into distinct groups. \n");
m->mothurOut("For the classification method, you need to provide your distance file and taxonomy file, and set the splitmethod to classify. \n");
- m->mothurOut("You will also need to set the taxlevel you want to split by. mothur will split the sequence into distinct taxonomy groups, and split the distance file based on those groups. \n");
+ m->mothurOut("You will also need to set the taxlevel you want to split by. mothur will split the sequences into distinct taxonomy groups, and split the distance file based on those groups. \n");
m->mothurOut("For the classification method using a fasta file, you need to provide your fasta file, names file and taxonomy file. \n");
m->mothurOut("You will also need to set the taxlevel you want to split by. mothur will split the sequence into distinct taxonomy groups, and create distance files for each grouping. \n");
m->mothurOut("The phylip and column parameter allow you to enter your distance file. \n");
m->mothurOut("The method allows you to specify what clustering algorythm you want to use, default=furthest, option furthest, nearest, or average. \n");
m->mothurOut("The splitmethod parameter allows you to specify how you want to split your distance file before you cluster, default=distance, options distance, classify or fasta. \n");
m->mothurOut("The taxonomy parameter allows you to enter the taxonomy file for your sequences, this is only valid if you are using splitmethod=classify. Be sure your taxonomy file does not include the probability scores. \n");
- m->mothurOut("The taxlevel parameter allows you to specify the taxonomy level you want to use to split the distance file, default=1. \n");
+ m->mothurOut("The taxlevel parameter allows you to specify the taxonomy level you want to use to split the distance file, default=1, meaning use the first taxon in each list. \n");
m->mothurOut("The large parameter allows you to indicate that your distance matrix is too large to fit in RAM. The default value is false.\n");
#ifdef USE_MPI
m->mothurOut("When using MPI, the processors parameter is set to the number of MPI processes running. \n");
vector<string> listFileNames;
set<string> labels;
string singletonName = "";
+ double saveCutoff = cutoff;
//****************** file prep work ******************************//
#ifdef USE_MPI
if (namefile == "") { //you need to make a namefile for split matrix
ofstream out;
namefile = phylipfile + ".names";
- openOutputFile(namefile, out);
+ m->openOutputFile(namefile, out);
for (int i = 0; i < listToMakeNameFile->getNumBins(); i++) {
string bin = listToMakeNameFile->get(i);
out << bin << '\t' << bin << endl;
SplitMatrix* split;
if (splitmethod == "distance") { split = new SplitMatrix(distfile, namefile, taxFile, cutoff, splitmethod, large); }
else if (splitmethod == "classify") { split = new SplitMatrix(distfile, namefile, taxFile, taxLevelCutoff, splitmethod, large); }
- else if (splitmethod == "fasta") { split = new SplitMatrix(fastafile, namefile, taxFile, taxLevelCutoff, splitmethod, processors, outputDir); }
+ else if (splitmethod == "fasta") { split = new SplitMatrix(fastafile, namefile, taxFile, taxLevelCutoff, cutoff, splitmethod, processors, outputDir); }
else { m->mothurOut("Not a valid splitting method. Valid splitting algorithms are distance, classify or fasta."); m->mothurOutEndLine(); return 0; }
split->split();
vector< map<string, string> > distName = split->getDistanceFiles(); //returns map of distance files -> namefile sorted by distance file size
delete split;
+ //output a merged distance file
+ if (splitmethod == "fasta") { createMergedDistanceFile(distName); }
+
+
if (m->control_pressed) { return 0; }
m->mothurOut("It took " + toString(time(NULL) - estart) + " seconds to split the distance file."); m->mothurOutEndLine();
for(int i = 1; i < processors; i++) {
int num = dividedNames[i].size();
+ double tempCutoff;
+ MPI_Recv(&tempCutoff, 1, MPI_DOUBLE, i, tag, MPI_COMM_WORLD, &status);
+ if (tempCutoff < cutoff) { cutoff = tempCutoff; }
+
//send list filenames to root process
for (int j = 0; j < num; j++) {
int lengthList = 0;
//process them
listFileNames = cluster(myNames, labels);
+ //send cutoff
+ MPI_Send(&cutoff, 1, MPI_DOUBLE, 0, tag, MPI_COMM_WORLD);
+
//send list filenames to root process
for (int j = 0; j < num; j++) {
char tempListFileName[1024];
//for each file group figure out which process will complete it
//want to divide the load intelligently so the big files are spread between processes
- int count = 1;
for (int i = 0; i < distName.size(); i++) {
int processToAssign = (i+1) % processors;
if (processToAssign == 0) { processToAssign = processors; }
for(int i=0;i<processors;i++){
string filename = toString(processIDS[i]) + ".temp";
ifstream in;
- openInputFile(filename, in);
+ m->openInputFile(filename, in);
- in >> tag; gobble(in);
+ in >> tag; m->gobble(in);
while(!in.eof()) {
string tempName;
- in >> tempName; gobble(in);
+ in >> tempName; m->gobble(in);
listFileNames.push_back(tempName);
}
in.close();
//get labels
filename = toString(processIDS[i]) + ".temp.labels";
ifstream in2;
- openInputFile(filename, in2);
+ m->openInputFile(filename, in2);
+
+ float tempCutoff;
+ in2 >> tempCutoff; m->gobble(in2);
+ if (tempCutoff < cutoff) { cutoff = tempCutoff; }
while(!in2.eof()) {
string tempName;
- in2 >> tempName; gobble(in2);
+ in2 >> tempName; m->gobble(in2);
if (labels.count(tempName) == 0) { labels.insert(tempName); }
}
in2.close();
#endif
if (m->control_pressed) { for (int i = 0; i < listFileNames.size(); i++) { remove(listFileNames[i].c_str()); } return 0; }
+ if (saveCutoff != cutoff) { m->mothurOut("Cutoff was " + toString(saveCutoff) + " changed cutoff to " + toString(cutoff)); m->mothurOutEndLine(); }
+
m->mothurOut("It took " + toString(time(NULL) - estart) + " seconds to cluster"); m->mothurOutEndLine();
//****************** merge list file and create rabund and sabund files ******************************//
//read in singletons
if (singleton != "none") {
ifstream in;
- openInputFile(singleton, in);
+ m->openInputFile(singleton, in);
string firstCol, secondCol;
listSingle = new ListVector();
while (!in.eof()) {
- in >> firstCol >> secondCol; gobble(in);
+ in >> firstCol >> secondCol; m->gobble(in);
listSingle->push_back(secondCol);
}
in.close();
if ((*it != "unique") && (convertTestFloat(*it, temp) == true)) { convert(*it, temp); }
else if (*it == "unique") { temp = -1.0; }
-
- orderFloat.push_back(temp);
- labelBin[temp] = numSingleBins; //initialize numbins
+
+ if (temp <= cutoff) {
+ orderFloat.push_back(temp);
+ labelBin[temp] = numSingleBins; //initialize numbins
+ }
}
//sort order
string filledInList = listNames[k] + "filledInTemp";
ofstream outFilled;
- openOutputFile(filledInList, outFilled);
+ m->openOutputFile(filledInList, outFilled);
//for each label needed
for(int l = 0; l < orderFloat.size(); l++){
//**********************************************************************************************************************
int ClusterSplitCommand::mergeLists(vector<string> listNames, map<float, int> userLabels, ListVector* listSingle){
try {
- if (outputDir == "") { outputDir += hasPath(distfile); }
- fileroot = outputDir + getRootName(getSimpleName(distfile));
+ if (outputDir == "") { outputDir += m->hasPath(distfile); }
+ fileroot = outputDir + m->getRootName(m->getSimpleName(distfile));
- openOutputFile(fileroot+ tag + ".sabund", outSabund);
- openOutputFile(fileroot+ tag + ".rabund", outRabund);
- openOutputFile(fileroot+ tag + ".list", outList);
+ m->openOutputFile(fileroot+ tag + ".sabund", outSabund);
+ m->openOutputFile(fileroot+ tag + ".rabund", outRabund);
+ m->openOutputFile(fileroot+ tag + ".list", outList);
- outputNames.push_back(fileroot+ tag + ".sabund");
- outputNames.push_back(fileroot+ tag + ".rabund");
- outputNames.push_back(fileroot+ tag + ".list");
+ outputNames.push_back(fileroot+ tag + ".sabund"); outputTypes["list"].push_back(fileroot+ tag + ".list");
+ outputNames.push_back(fileroot+ tag + ".rabund"); outputTypes["rabund"].push_back(fileroot+ tag + ".rabund");
+ outputNames.push_back(fileroot+ tag + ".list"); outputTypes["sabund"].push_back(fileroot+ tag + ".sabund");
map<float, int>::iterator itLabel;
if (listSingle != NULL) {
for (int j = 0; j < listSingle->getNumBins(); j++) {
outList << listSingle->get(j) << '\t';
- rabund->push_back(getNumNames(listSingle->get(j)));
+ rabund->push_back(m->getNumNames(listSingle->get(j)));
}
}
else {
for (int j = 0; j < list->getNumBins(); j++) {
outList << list->get(j) << '\t';
- rabund->push_back(getNumNames(list->get(j)));
+ rabund->push_back(m->getNumNames(list->get(j)));
}
delete list;
}
RAbundVector oldRAbund = oldList->getRAbundVector();
oldRAbund.setLabel(label);
- if (isTrue(showabund)) {
+ if (m->isTrue(showabund)) {
oldRAbund.getSAbundVector().print(cout);
}
oldRAbund.print(outRabund);
//write out names to file
string filename = toString(getpid()) + ".temp";
ofstream out;
- openOutputFile(filename, out);
+ m->openOutputFile(filename, out);
out << tag << endl;
for (int j = 0; j < listFileNames.size(); j++) { out << listFileNames[j] << endl; }
out.close();
//print out labels
ofstream outLabels;
filename = toString(getpid()) + ".temp.labels";
- openOutputFile(filename, outLabels);
-
+ m->openOutputFile(filename, outLabels);
+
+ outLabels << cutoff << endl;
for (set<string>::iterator it = labels.begin(); it != labels.end(); it++) {
outLabels << (*it) << endl;
}
outLabels.close();
exit(0);
- }else { m->mothurOut("unable to spawn the necessary processes."); m->mothurOutEndLine(); exit(0); }
+ }else {
+ m->mothurOut("[ERROR]: unable to spawn the necessary processes."); m->mothurOutEndLine();
+ for (int i = 0; i < processIDS.size(); i++) { kill (processIDS[i], SIGINT); }
+ exit(0);
+ }
}
//force parent to wait until all the processes are done
vector<string> listFileNames;
+ double smallestCutoff = cutoff;
+
//cluster each distance file
for (int i = 0; i < distNames.size(); i++) {
+ if (m->control_pressed) { return listFileNames; }
string thisNamefile = distNames[i].begin()->second;
string thisDistFile = distNames[i].begin()->first;
else if(method == "average"){ cluster = new AverageLinkage(rabund, list, matrix, cutoff, method); }
tag = cluster->getTag();
- if (outputDir == "") { outputDir += hasPath(thisDistFile); }
- fileroot = outputDir + getRootName(getSimpleName(thisDistFile));
+ if (outputDir == "") { outputDir += m->hasPath(thisDistFile); }
+ fileroot = outputDir + m->getRootName(m->getSimpleName(thisDistFile));
ofstream listFile;
- openOutputFile(fileroot+ tag + ".list", listFile);
+ m->openOutputFile(fileroot+ tag + ".list", listFile);
listFileNames.push_back(fileroot+ tag + ".list");
-
- time_t estart = time(NULL);
-
+
float previousDist = 0.00000;
float rndPreviousDist = 0.00000;
listFileNames.clear(); return listFileNames;
}
- cluster->update(cutoff);
+ cluster->update(saveCutoff);
float dist = matrix->getSmallDist();
float rndDist;
if (hard) {
- rndDist = ceilDist(dist, precision);
+ rndDist = m->ceilDist(dist, precision);
}else{
- rndDist = roundDist(dist, precision);
+ rndDist = m->roundDist(dist, precision);
}
if(previousDist <= 0.0000 && dist != previousDist){
remove(thisDistFile.c_str());
remove(thisNamefile.c_str());
+
+ if (saveCutoff != cutoff) {
+ if (hard) { saveCutoff = m->ceilDist(saveCutoff, precision); }
+ else { saveCutoff = m->roundDist(saveCutoff, precision); }
+
+ m->mothurOut("Cutoff was " + toString(cutoff) + " changed cutoff to " + toString(saveCutoff)); m->mothurOutEndLine();
+ }
+
+ if (saveCutoff < smallestCutoff) { smallestCutoff = saveCutoff; }
}
+ cutoff = smallestCutoff;
return listFileNames;
}
+//**********************************************************************************************************************
+int ClusterSplitCommand::createMergedDistanceFile(vector< map<string, string> > distNames) {
+ try{
+
+#ifdef USE_MPI
+ int pid;
+ MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
+
+ if (pid != 0) {
+#endif
+
+ string thisOutputDir = outputDir;
+ if (outputDir == "") { thisOutputDir = m->hasPath(fastafile); }
+ string outputFileName = thisOutputDir + m->getRootName(m->getSimpleName(fastafile)) + "dist";
+ remove(outputFileName.c_str());
+
+
+ for (int i = 0; i < distNames.size(); i++) {
+ if (m->control_pressed) { return 0; }
+
+ string thisDistFile = distNames[i].begin()->first;
+
+ m->appendFiles(thisDistFile, outputFileName);
+ }
+
+ outputTypes["column"].push_back(outputFileName); outputNames.push_back(outputFileName);
+
+#ifdef USE_MPI
+ }
+#endif
+
+
+
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "ClusterSplitCommand", "createMergedDistanceFile");
+ exit(1);
+ }
+}
//**********************************************************************************************************************
projects / mothur.git / blobdiff