#include "kmerdb.hpp"
#include "suffixdb.hpp"
#include "blastdb.hpp"
+#include "distancedb.hpp"
/**************************************************************************************************/
-
-Classify::Classify(string tfile, string tempFile, string method, int kmerSize, int gapOpen, int gapExtend, int match, int misMatch) : taxFile(tfile), templateFile(tempFile) {
- try {
+void Classify::generateDatabaseAndNames(string tfile, string tempFile, string method, int kmerSize, float gapOpen, float gapExtend, float match, float misMatch) {
+ try {
+ taxFile = tfile;
+ readTaxonomy(taxFile);
- readTaxonomy(taxFile);
+ templateFile = tempFile;
+ int start = time(NULL);
int numSeqs = 0;
+
+ m->mothurOut("Generating search database... "); cout.flush();
+#ifdef USE_MPI
+ int pid, processors;
+ vector<unsigned long int> positions;
+ int tag = 2001;
+
+ MPI_Status status;
+ MPI_File inMPI;
+ MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
+ MPI_Comm_size(MPI_COMM_WORLD, &processors);
+
+ //char* inFileName = new char[tempFile.length()];
+ //memcpy(inFileName, tempFile.c_str(), tempFile.length());
+
+ char inFileName[1024];
+ strcpy(inFileName, tempFile.c_str());
+
+ MPI_File_open(MPI_COMM_WORLD, inFileName, MPI_MODE_RDONLY, MPI_INFO_NULL, &inMPI); //comm, filename, mode, info, filepointer
+ //delete inFileName;
+
+ if (pid == 0) { //only one process needs to scan file
+ positions = m->setFilePosFasta(tempFile, numSeqs); //fills MPIPos, returns numSeqs
+
+ //send file positions to all processes
+ for(int i = 1; i < processors; i++) {
+ MPI_Send(&numSeqs, 1, MPI_INT, i, tag, MPI_COMM_WORLD);
+ MPI_Send(&positions[0], (numSeqs+1), MPI_LONG, i, tag, MPI_COMM_WORLD);
+ }
+ }else{
+ MPI_Recv(&numSeqs, 1, MPI_INT, 0, tag, MPI_COMM_WORLD, &status);
+ positions.resize(numSeqs+1);
+ MPI_Recv(&positions[0], (numSeqs+1), MPI_LONG, 0, tag, MPI_COMM_WORLD, &status);
+ }
+
+ //create database
+ if(method == "kmer") { database = new KmerDB(tempFile, kmerSize); }
+ else if(method == "suffix") { database = new SuffixDB(numSeqs); }
+ else if(method == "blast") { database = new BlastDB(gapOpen, gapExtend, match, misMatch); }
+ else if(method == "distance") { database = new DistanceDB(); }
+ else {
+ m->mothurOut(method + " is not a valid search option. I will run the command using kmer, ksize=8."); m->mothurOutEndLine();
+ database = new KmerDB(tempFile, 8);
+ }
+
+ //read file
+ for(int i=0;i<numSeqs;i++){
+ //read next sequence
+ int length = positions[i+1] - positions[i];
+ char* buf4 = new char[length];
+ MPI_File_read_at(inMPI, positions[i], buf4, length, MPI_CHAR, &status);
+
+ string tempBuf = buf4;
+ if (tempBuf.length() > length) { tempBuf = tempBuf.substr(0, length); }
+ delete buf4;
+ istringstream iss (tempBuf,istringstream::in);
+
+ Sequence temp(iss);
+ if (temp.getName() != "") {
+ names.push_back(temp.getName());
+ database->addSequence(temp);
+ }
+ }
+
+ database->generateDB();
+ MPI_File_close(&inMPI);
+ MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case
+ #else
+
//need to know number of template seqs for suffixdb
if (method == "suffix") {
ifstream inFASTA;
- openInputFile(tempFile, inFASTA);
- numSeqs = count(istreambuf_iterator<char>(inFASTA),istreambuf_iterator<char>(), '>');
+ m->openInputFile(tempFile, inFASTA);
+ m->getNumSeqs(inFASTA, numSeqs);
inFASTA.close();
}
- mothurOut("Generating search database... "); cout.flush();
-
bool needToGenerate = true;
string kmerDBName;
if(method == "kmer") {
kmerDBName = tempFile.substr(0,tempFile.find_last_of(".")+1) + char('0'+ kmerSize) + "mer";
ifstream kmerFileTest(kmerDBName.c_str());
- if(kmerFileTest){ needToGenerate = false; }
+ if(kmerFileTest){
+ bool GoodFile = m->checkReleaseVersion(kmerFileTest, m->getVersion());
+ if (GoodFile) { needToGenerate = false; }
+ }
}
else if(method == "suffix") { database = new SuffixDB(numSeqs); }
else if(method == "blast") { database = new BlastDB(gapOpen, gapExtend, match, misMatch); }
+ else if(method == "distance") { database = new DistanceDB(); }
else {
- mothurOut(method + " is not a valid search option. I will run the command using kmer, ksize=8.");
- mothurOutEndLine();
+ m->mothurOut(method + " is not a valid search option. I will run the command using kmer, ksize=8.");
+ m->mothurOutEndLine();
database = new KmerDB(tempFile, 8);
}
if (needToGenerate) {
ifstream fastaFile;
- openInputFile(tempFile, fastaFile);
-
+ m->openInputFile(tempFile, fastaFile);
+
while (!fastaFile.eof()) {
Sequence temp(fastaFile);
- gobble(fastaFile);
+ m->gobble(fastaFile);
names.push_back(temp.getName());
+
database->addSequence(temp);
}
fastaFile.close();
}else if ((method == "kmer") && (!needToGenerate)) {
ifstream kmerFileTest(kmerDBName.c_str());
database->readKmerDB(kmerFileTest);
-
- ifstream fastaFile;
- openInputFile(tempFile, fastaFile);
+ ifstream fastaFile;
+ m->openInputFile(tempFile, fastaFile);
+
while (!fastaFile.eof()) {
Sequence temp(fastaFile);
- gobble(fastaFile);
-
+ m->gobble(fastaFile);
+
names.push_back(temp.getName());
}
fastaFile.close();
}
-
+#endif
+
database->setNumSeqs(names.size());
- mothurOut("DONE."); mothurOutEndLine();
+ //sanity check
+ bool okay = phyloTree->ErrorCheck(names);
+
+ if (!okay) { m->control_pressed = true; }
+
+ m->mothurOut("DONE."); m->mothurOutEndLine();
+ m->mothurOut("It took " + toString(time(NULL) - start) + " seconds generate search database. "); m->mothurOutEndLine();
}
catch(exception& e) {
- errorOut(e, "Classify", "Classify");
+ m->errorOut(e, "Classify", "generateDatabaseAndNames");
exit(1);
}
}
/**************************************************************************************************/
+Classify::Classify() { m = MothurOut::getInstance(); database = NULL; }
+/**************************************************************************************************/
-void Classify::readTaxonomy(string file) {
+int Classify::readTaxonomy(string file) {
try {
+
+ phyloTree = new PhyloTree();
+ string name, taxInfo;
+
+ m->mothurOutEndLine();
+ m->mothurOut("Reading in the " + file + " taxonomy...\t"); cout.flush();
+
+#ifdef USE_MPI
+ int pid, num, processors;
+ vector<unsigned long int> positions;
+ int tag = 2001;
+
+ MPI_Status status;
+ MPI_File inMPI;
+ MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
+ MPI_Comm_size(MPI_COMM_WORLD, &processors);
+
+ //char* inFileName = new char[file.length()];
+ //memcpy(inFileName, file.c_str(), file.length());
+
+ char inFileName[1024];
+ strcpy(inFileName, file.c_str());
+
+ MPI_File_open(MPI_COMM_WORLD, inFileName, MPI_MODE_RDONLY, MPI_INFO_NULL, &inMPI); //comm, filename, mode, info, filepointer
+ //delete inFileName;
+
+ if (pid == 0) {
+ positions = m->setFilePosEachLine(file, num);
+
+ //send file positions to all processes
+ for(int i = 1; i < processors; i++) {
+ MPI_Send(&num, 1, MPI_INT, i, tag, MPI_COMM_WORLD);
+ MPI_Send(&positions[0], (num+1), MPI_LONG, i, tag, MPI_COMM_WORLD);
+ }
+ }else{
+ MPI_Recv(&num, 1, MPI_INT, 0, tag, MPI_COMM_WORLD, &status);
+ positions.resize(num+1);
+ MPI_Recv(&positions[0], (num+1), MPI_LONG, 0, tag, MPI_COMM_WORLD, &status);
+ }
+ //read file
+ for(int i=0;i<num;i++){
+ //read next sequence
+ int length = positions[i+1] - positions[i];
+ char* buf4 = new char[length];
+
+ MPI_File_read_at(inMPI, positions[i], buf4, length, MPI_CHAR, &status);
+
+ string tempBuf = buf4;
+ if (tempBuf.length() > length) { tempBuf = tempBuf.substr(0, length); }
+ delete buf4;
+
+ istringstream iss (tempBuf,istringstream::in);
+ iss >> name >> taxInfo;
+ taxonomy[name] = taxInfo;
+ phyloTree->addSeqToTree(name, taxInfo);
+ }
+
+ MPI_File_close(&inMPI);
+ MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case
+#else
ifstream inTax;
- openInputFile(file, inTax);
+ m->openInputFile(file, inTax);
- mothurOutEndLine();
- mothurOut("Reading in the " + file + " taxonomy...\t"); cout.flush();
-
- string name, taxInfo;
//read template seqs and save
while (!inTax.eof()) {
inTax >> name >> taxInfo;
taxonomy[name] = taxInfo;
+
+ phyloTree->addSeqToTree(name, taxInfo);
- gobble(inTax);
+ m->gobble(inTax);
}
inTax.close();
+#endif
- mothurOut("DONE.");
- mothurOutEndLine(); cout.flush();
+ phyloTree->assignHeirarchyIDs(0);
+
+ phyloTree->setUp(file);
+ m->mothurOut("DONE.");
+ m->mothurOutEndLine(); cout.flush();
+
+ return phyloTree->getNumSeqs();
+
+ }
+ catch(exception& e) {
+ m->errorOut(e, "Classify", "readTaxonomy");
+ exit(1);
+ }
+}
+/**************************************************************************************************/
+
+vector<string> Classify::parseTax(string tax) {
+ try {
+ vector<string> taxons;
+
+ tax = tax.substr(0, tax.length()-1); //get rid of last ';'
+
+ //parse taxonomy
+ string individual;
+ while (tax.find_first_of(';') != -1) {
+ individual = tax.substr(0,tax.find_first_of(';'));
+ tax = tax.substr(tax.find_first_of(';')+1, tax.length());
+ taxons.push_back(individual);
+
+ }
+ //get last one
+ taxons.push_back(tax);
+
+ return taxons;
}
catch(exception& e) {
- errorOut(e, "Classify", "readTaxonomy");
+ m->errorOut(e, "Classify", "parseTax");
exit(1);
}
}
projects / mothur.git / blobdiff